Rh1AG241600

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0014799
Physical Location & Seq
Reverse (-)
45277641 .. 45281328
3688 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG241600.1

Sequence Viewer

Length: 831 bp
ATGGAAGCAGTACATGATATTTGCAAACTCTATTTTGCACGATTTGCTGCAGCATTAAATAGAGGACATTTCTTTTGTTTAGCTCTCGTGGCTTTTCTCTTTCCTCTGTTTACCTTTCACGAATTTATAGTTGATCACCCCGCCTGTTCATATGAATTGGCAGCCAGATTAAACAAAATTACATACTTTCCAGGCTCAGAAATCATTGATGTTGAACAGCAGCAGAGGAGCAATGGTGACAGCTTTTTCGATATTGAAGAGCTCCTGCAGATTGGGACCAGATGCAGAGGGGAATTAACTGAATTATATCCAACCAAGCAGCTTAAGAAGTTCTTTAGATTTACTGATAAGCCTGATATATGGAGTGTGGGGTGCACTGTTATTGAGATGGCCACTGGAGATCCTTGGACTCTTGGAGGGGAAATTACCACGCAGGGGCCTCTTTTCTCAAACATATGGAGAAATCAGATTCTGTTCCTCCAATTAATCCCCGATAATCTTTCTTCTTATGGGAAACAATTTTTGAGACGATGTCTGCAAAGGGAACCAAATTTGAGGGCATCTGCATCCCAGTTGCTTGAGGATCCATTCATATCCAGGAAAAACTTCAGCCACCCATCTGGAGCTGGTACAGCTGTTGGAGCTGTTGCTGCCCCCGTCATCCACCAGCAACACGTAGTTACCCCAGCTGCTCCCAAGCAACGCATTGTTACCCCGCTGCTCCCTCCCAAGCAACACATGGTTACCCCTGCTGCTCCCAAGCAGCGCATTGTTACCCCATCCACACAACGCGTTGTTCACTCGATCAGTTGCTTCACGTCCTGCTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

276

Amino Acids

31.04

Weight (kDa)

8.58

Isoelectric Point (pI)

47.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 115 - 198 6.5e-10 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 792
AciI CCGC 2 cut(s) 141, 716
AclWI GGATC 3 cut(s) 395, 578, 591
AcoI YGGCCR 1 cut(s) 390
AcsI RAATTY 2 cut(s) 122, 550
AcuI CTGAAG 1 cut(s) 592
AfaI GTAC 2 cut(s) 12, 631
AfiI CCNNNNNNNGG 1 cut(s) 435
AflII CTTAAG 1 cut(s) 323
AflIII ACRYGT 2 cut(s) 673, 790
AgsI TTSAA 2 cut(s) 215, 257
AjiI CACGTC 1 cut(s) 819
AjnI CCWGG 2 cut(s) 190, 596
AluBI AGCT 8 cut(s) 83, 243, 262, 322, 626, 635, 644, 689
AluI AGCT 8 cut(s) 83, 243, 262, 322, 626, 635, 644, 689
Alw21I GWGCWC 2 cut(s) 264, 377
Alw26I GTCTC 1 cut(s) 520
Alw44I GTGCAC 1 cut(s) 373
AlwI GGATC 3 cut(s) 395, 578, 591
AlwNI CAGNNNCTG 1 cut(s) 472
AoxI GGCC 2 cut(s) 390, 437
ApaLI GTGCAC 1 cut(s) 373
ApoI RAATTY 2 cut(s) 122, 550
ArsI GACNNNNNNTTYG 4 cut(s) 57, 89, 230, 262
AseI ATTAAT 1 cut(s) 485
Asp700I GAANNNNTTC 1 cut(s) 605
AspLEI GCGC 1 cut(s) 768
AspS9I GGNCC 2 cut(s) 276, 437
AsuHPI GGTGA 2 cut(s) 128, 248
AvaII GGWCC 1 cut(s) 276
BaeGI GKGCMC 1 cut(s) 377
BaeI ACNNNNGTAYC 2 cut(s) 621, 654
BalI TGGCCA 1 cut(s) 392
BamHI GGATCC 1 cut(s) 583
BanII GRGCYC 1 cut(s) 264
BauI CACGAG 1 cut(s) 86
Bbv12I GWGCWC 2 cut(s) 264, 377
BccI CCATC 3 cut(s) 382, 625, 787
BciT130I CCWGG 2 cut(s) 192, 598
BclI TGATCA 1 cut(s) 133
BcoDI GTCTC 1 cut(s) 520
BfaI CTAG 1 cut(s) 829
BfmI CTRYAG 2 cut(s) 48, 266
BfrI CTTAAG 1 cut(s) 323
Bme1390I CCNGG 2 cut(s) 192, 598
Bme18I GGWCC 1 cut(s) 276
BmgBI CACGTC 1 cut(s) 819
BmgT120I GGNCC 2 cut(s) 276, 437
BmiI GGNNCC 4 cut(s) 277, 438, 546, 585
BmrFI CCNGG 2 cut(s) 192, 598
BmrI ACTGGG 1 cut(s) 565
BmsI GCATC 3 cut(s) 272, 569, 575
BmuI ACTGGG 1 cut(s) 565
BpmI CTGGAG 2 cut(s) 417, 642
BpuEI CTTGAG 1 cut(s) 599
BsaAI YACGTR 1 cut(s) 676
BsaJI CCNNGG 1 cut(s) 404
BsaXI ACNNNNNCTCC 2 cut(s) 220, 250
Bsc4I CCNNNNNNNGG 1 cut(s) 435
Bse1I ACTGG 2 cut(s) 400, 571
Bse3DI GCAATG 1 cut(s) 238
BseBI CCWGG 2 cut(s) 192, 598
BseDI CCNNGG 1 cut(s) 404
BseGI GGATG 3 cut(s) 566, 660, 779
BseLI CCNNNNNNNGG 1 cut(s) 435
BseMI GCAATG 1 cut(s) 238
BseMII CTCAG 1 cut(s) 210
BseNI ACTGG 2 cut(s) 400, 571
BseRI GAGGAG 1 cut(s) 241
BseSI GKGCMC 1 cut(s) 377
BseYI CCCAGC 1 cut(s) 685
Bsh1236I CGCG 1 cut(s) 792
BshFI GGCC 2 cut(s) 392, 439
BsiHKAI GWGCWC 2 cut(s) 264, 377
BslFI GGGAC 1 cut(s) 289
BslI CCNNNNNNNGG 1 cut(s) 435
BsmAI GTCTC 1 cut(s) 520
BsmBI CGTCTC 1 cut(s) 520
BsmFI GGGAC 1 cut(s) 289
BsnI GGCC 2 cut(s) 392, 439
Bsp1286I GDGCHC 2 cut(s) 264, 377
Bsp143I GATC 4 cut(s) 133, 400, 583, 804
BspACI CCGC 2 cut(s) 141, 716
BspANI GGCC 2 cut(s) 392, 439
BspCNI CTCAG 1 cut(s) 209
BspFNI CGCG 1 cut(s) 792
BspLI GGNNCC 4 cut(s) 277, 438, 546, 585
BspMAI CTGCAG 2 cut(s) 52, 270
BspPI GGATC 3 cut(s) 395, 578, 591
BspQI GCTCTTC 1 cut(s) 252
BspTI CTTAAG 1 cut(s) 323
BsrDI GCAATG 1 cut(s) 238
BsrI ACTGG 2 cut(s) 400, 571
BssECI CCNNGG 1 cut(s) 404
BssMI GATC 4 cut(s) 133, 400, 583, 804
BssSI CACGAG 1 cut(s) 86
BssT1I CCWWGG 1 cut(s) 404
Bst2BI CACGAG 1 cut(s) 86
Bst2UI CCWGG 2 cut(s) 192, 598
Bst4CI ACNGT 1 cut(s) 379
Bst6I CTCTTC 1 cut(s) 252
BstAFI CTTAAG 1 cut(s) 323
BstAPI GCANNNNNTGC 1 cut(s) 44
BstBAI YACGTR 1 cut(s) 676
BstDEI CTNAG 1 cut(s) 196
BstEII GGTNACC 1 cut(s) 742
BstF5I GGATG 3 cut(s) 566, 660, 779
BstFNI CGCG 1 cut(s) 792
BstHHI GCGC 1 cut(s) 768
BstKTI GATC 4 cut(s) 136, 403, 586, 807
BstMAI GTCTC 1 cut(s) 520
BstMBI GATC 4 cut(s) 133, 400, 583, 804
BstMWI GCNNNNNNNGC 5 cut(s) 44, 89, 632, 641, 650
BstNI CCWGG 2 cut(s) 192, 598
BstPI GGTNACC 1 cut(s) 742
BstSCI CCNGG 2 cut(s) 190, 596
BstSFI CTRYAG 2 cut(s) 48, 266
BstSLI GKGCMC 1 cut(s) 377
BstUI CGCG 1 cut(s) 792
BstX2I RGATCY 2 cut(s) 400, 583
BstXI CCANNNNNNTGG 1 cut(s) 620
BstYI RGATCY 2 cut(s) 400, 583
BsuRI GGCC 2 cut(s) 392, 439
BtrI CACGTC 1 cut(s) 819
BtsCI GGATG 3 cut(s) 566, 660, 779
BtsIMutI CAGTG 2 cut(s) 375, 393
CaiI CAGNNNCTG 1 cut(s) 472
CfoI GCGC 1 cut(s) 768
Cfr13I GGNCC 2 cut(s) 276, 437
Csp6I GTAC 2 cut(s) 11, 630
CviAII CATG 2 cut(s) 14, 739
CviQI GTAC 2 cut(s) 11, 630
DdeI CTNAG 1 cut(s) 196
DpnI GATC 4 cut(s) 135, 402, 585, 806
DpnII GATC 4 cut(s) 133, 400, 583, 804
EaeI YGGCCR 1 cut(s) 390
Eam1104I CTCTTC 1 cut(s) 252
EarI CTCTTC 1 cut(s) 252
Ecl136II GAGCTC 1 cut(s) 262
Eco130I CCWWGG 1 cut(s) 404
Eco24I GRGCYC 1 cut(s) 264
Eco47I GGWCC 1 cut(s) 276
Eco53kI GAGCTC 1 cut(s) 262
Eco57I CTGAAG 1 cut(s) 592
Eco91I GGTNACC 1 cut(s) 742
EcoICRI GAGCTC 1 cut(s) 262
EcoO109I RGGNCCY 1 cut(s) 437
EcoO65I GGTNACC 1 cut(s) 742
EcoRII CCWGG 2 cut(s) 190, 596
EcoT14I CCWWGG 1 cut(s) 404
EcoT38I GRGCYC 1 cut(s) 264
ErhI CCWWGG 1 cut(s) 404
Esp3I CGTCTC 1 cut(s) 520
FaeI CATG 2 cut(s) 17, 742
FalI AAGNNNNNCTT 2 cut(s) 317, 349
FaqI GGGAC 1 cut(s) 289
FatI CATG 2 cut(s) 13, 738
FauI CCCGC 2 cut(s) 148, 723
FauNDI CATATG 2 cut(s) 151, 455
FbaI TGATCA 1 cut(s) 133
FokI GGATG 3 cut(s) 553, 647, 766
FriOI GRGCYC 1 cut(s) 264
FspBI CTAG 1 cut(s) 829
GlaI GCGC 1 cut(s) 767
GsaI CCCAGC 1 cut(s) 689
GsuI CTGGAG 2 cut(s) 417, 642
HaeIII GGCC 2 cut(s) 392, 439
HhaI GCGC 1 cut(s) 768
Hin1II CATG 2 cut(s) 17, 742
Hin6I GCGC 1 cut(s) 766
HinP1I GCGC 1 cut(s) 766
HinfI GANTC 2 cut(s) 409, 469
HphI GGTGA 2 cut(s) 128, 248
Hpy166II GTNNAC 3 cut(s) 111, 375, 799
Hpy188I TCNGA 2 cut(s) 199, 468
Hpy188III TCNNGA 2 cut(s) 119, 621
Hpy8I GTNNAC 3 cut(s) 111, 375, 799
HpyCH4III ACNGT 1 cut(s) 379
HpyCH4IV ACGT 2 cut(s) 675, 818
HpyCH4V TGCA 8 cut(s) 24, 38, 50, 268, 285, 375, 538, 566
HpyF10VI GCNNNNNNNGC 5 cut(s) 44, 89, 632, 641, 650
HpyF3I CTNAG 1 cut(s) 196
HpySE526I ACGT 2 cut(s) 675, 818
Hsp92II CATG 2 cut(s) 17, 742
HspAI GCGC 1 cut(s) 766
Ksp22I TGATCA 1 cut(s) 133
Kzo9I GATC 4 cut(s) 133, 400, 583, 804
LguI GCTCTTC 1 cut(s) 252
LmnI GCTCC 7 cut(s) 228, 267, 623, 641, 697, 726, 760
LweI GCATC 3 cut(s) 272, 569, 575
MaeI CTAG 1 cut(s) 829
MaeII ACGT 2 cut(s) 675, 818
MaeIII GTNAC 5 cut(s) 236, 679, 709, 742, 772
MalI GATC 4 cut(s) 135, 402, 585, 806
MboI GATC 4 cut(s) 133, 400, 583, 804
MboII GAAGA 2 cut(s) 269, 495
MflI RGATCY 2 cut(s) 400, 583
MhlI GDGCHC 2 cut(s) 264, 377
MlsI TGGCCA 1 cut(s) 392
MluCI AATT 9 cut(s) 122, 155, 177, 293, 302, 423, 482, 518, 550
MluI ACGCGT 1 cut(s) 790
MluNI TGGCCA 1 cut(s) 392
MlyI GAGTC 1 cut(s) 403
MmeI TCCRAC 2 cut(s) 335, 619
Mox20I TGGCCA 1 cut(s) 392
MroXI GAANNNNTTC 1 cut(s) 605
MscI TGGCCA 1 cut(s) 392
MseI TTAA 5 cut(s) 56, 170, 296, 324, 485
Msp20I TGGCCA 1 cut(s) 392
MspA1I CMGCKG 3 cut(s) 635, 689, 718
MspCI CTTAAG 1 cut(s) 323
MspR9I CCNGG 2 cut(s) 192, 598
MvaI CCWGG 2 cut(s) 192, 598
MvnI CGCG 1 cut(s) 792
MwoI GCNNNNNNNGC 5 cut(s) 44, 89, 632, 641, 650
NdeI CATATG 2 cut(s) 151, 455
NdeII GATC 4 cut(s) 133, 400, 583, 804
NlaIII CATG 2 cut(s) 17, 742
NlaIV GGNNCC 4 cut(s) 277, 438, 546, 585
NmuCI GTSAC 1 cut(s) 236
PciSI GCTCTTC 1 cut(s) 252
PdmI GAANNNNTTC 1 cut(s) 605
PfeI GAWTC 1 cut(s) 469
PflFI GACNNNGTC 1 cut(s) 531
PfoI TCCNGGA 1 cut(s) 596
PleI GAGTC 1 cut(s) 403
PpsI GAGTC 1 cut(s) 403
Ppu21I YACGTR 1 cut(s) 676
PshBI ATTAAT 1 cut(s) 485
Psp124BI GAGCTC 1 cut(s) 264
Psp6I CCWGG 2 cut(s) 190, 596
PspEI GGTNACC 1 cut(s) 742
PspFI CCCAGC 1 cut(s) 685
PspGI CCWGG 2 cut(s) 190, 596
PspN4I GGNNCC 4 cut(s) 277, 438, 546, 585
PspPI GGNCC 2 cut(s) 276, 437
PstI CTGCAG 2 cut(s) 52, 270
PstNI CAGNNNCTG 1 cut(s) 472
PsuI RGATCY 2 cut(s) 400, 583
PsyI GACNNNGTC 1 cut(s) 531
PvuII CAGCTG 2 cut(s) 635, 689
RsaI GTAC 2 cut(s) 12, 631
RsaNI GTAC 2 cut(s) 11, 630
SacI GAGCTC 1 cut(s) 264
SapI GCTCTTC 1 cut(s) 252
SaqAI TTAA 5 cut(s) 56, 170, 296, 324, 485
Sau3AI GATC 4 cut(s) 133, 400, 583, 804
Sau96I GGNCC 2 cut(s) 276, 437
SchI GAGTC 1 cut(s) 403
ScrFI CCNGG 2 cut(s) 192, 598
SduI GDGCHC 2 cut(s) 264, 377
SfaNI GCATC 3 cut(s) 272, 569, 575
SfcI CTRYAG 2 cut(s) 48, 266
SinI GGWCC 1 cut(s) 276
SmlI CTYRAG 2 cut(s) 323, 578
SmoI CTYRAG 2 cut(s) 323, 578
Sse9I AATT 9 cut(s) 122, 155, 177, 293, 302, 423, 482, 518, 550
SsiI CCGC 2 cut(s) 141, 716
SspMI CTAG 1 cut(s) 829
SstI GAGCTC 1 cut(s) 264
StyD4I CCNGG 2 cut(s) 190, 596
StyI CCWWGG 1 cut(s) 404
TaaI ACNGT 1 cut(s) 379
TaiI ACGT 2 cut(s) 678, 821
TaqI TCGA 2 cut(s) 249, 803
TasI AATT 9 cut(s) 122, 155, 177, 293, 302, 423, 482, 518, 550
TatI WGTACW 1 cut(s) 10
TfiI GAWTC 1 cut(s) 469
Tru1I TTAA 5 cut(s) 56, 170, 296, 324, 485
Tru9I TTAA 5 cut(s) 56, 170, 296, 324, 485
TscAI CASTG 2 cut(s) 382, 400
TseFI GTSAC 1 cut(s) 236
Tsp45I GTSAC 1 cut(s) 236
TspDTI ATGAA 3 cut(s) 138, 168, 580
TspRI CASTG 2 cut(s) 382, 400
Tth111I GACNNNGTC 1 cut(s) 531
Vha464I CTTAAG 1 cut(s) 323
VneI GTGCAC 1 cut(s) 373
VpaK11BI GGWCC 1 cut(s) 276
VspI ATTAAT 1 cut(s) 485
XapI RAATTY 2 cut(s) 122, 550
XcmI CCANNNNNNNNNTGG 1 cut(s) 736
XmnI GAANNNNTTC 1 cut(s) 605
XspI CTAG 1 cut(s) 829
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.