Rorug02G0075500

small GTPase mediated signal transduction

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0005576
Physical Location & Seq
Reverse (-)
5981520 .. 5982119
600 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0075500.1

Sequence Viewer

Length: 600 bp
ATGACGACCTACGGAACCATCCCGACAGAACGTTTGCCATCCTCGAACCTAGATATCGTTTCACAAGCAAAAGGACAGATTGAATTATGCCTTGGCATACAAAGACCATGGCTGGAGATGATTCAGCTCCAAGATCTCAAACCTCCCTCCTCCTTCAACCAATCAATCCAAAGAATCAGGACAAACGTAGCCTTCTTCCGCATGAACTACGCCATTATCATATTGACCATTCTCTTCATAAGCCTTCTATGGCACCCCATCTCATTGATGATCTTCATCGTCACGATGGCTGCATGGTTGTTTCTATATTATTTACACGACAAACCCTTGGTGGTTGTAGGCTATGAAATCGATCAACGATTCCTAATGGCAGCTTTGTTCTTGGTCACAGCCCTGATACTTTTCCTTACCAATGTGAAATATAACATTATAATGGCACTCTCTATTGGCCTTGCTTTTGTTTTGGTTCATGGAGCCTTAAGGGAGCCAGAGGATGTCTTGACTGTGGATGATGAAGAAGGGCTTGCATCAGGTGGTGGCCCTGTTCTAAAAGTCCCTCTAAAAAATGCTGCCTCTTCTTCTTATTCTCTGTCCCAATAG

Protein Analysis

199

Amino Acids

22.29

Weight (kDa)

5.88

Isoelectric Point (pI)

39.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRA1 PF03208 34 - 166 8.7e-42 PRA1 family protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 431
AccB1I GGYRCC 1 cut(s) 252
AciI CCGC 1 cut(s) 199
AclI AACGTT 1 cut(s) 31
AflII CTTAAG 1 cut(s) 478
AgsI TTSAA 2 cut(s) 83, 157
AjuI GAANNNNNNNTTGG 2 cut(s) 75, 107
AluBI AGCT 2 cut(s) 127, 374
AluI AGCT 2 cut(s) 127, 374
AoxI GGCC 2 cut(s) 448, 538
ApeKI GCWGC 3 cut(s) 290, 371, 569
ArsI GACNNNNNNTTYG 2 cut(s) 16, 48
AspS9I GGNCC 1 cut(s) 539
BanI GGYRCC 1 cut(s) 252
BbvI GCAGC 3 cut(s) 277, 383, 556
BccI CCATC 4 cut(s) 26, 46, 266, 280
BfaI CTAG 1 cut(s) 50
BfrI CTTAAG 1 cut(s) 478
BglII AGATCT 1 cut(s) 133
BisI GCNGC 3 cut(s) 291, 372, 570
BlsI GCNGC 3 cut(s) 292, 373, 571
BmgT120I GGNCC 1 cut(s) 539
BmiI GGNNCC 4 cut(s) 16, 254, 475, 486
BmsI GCATC 1 cut(s) 536
BpmI CTGGAG 1 cut(s) 134
Bsa29I ATCGAT 1 cut(s) 351
BsaBI GATNNNNATC 1 cut(s) 275
BsaJI CCNNGG 3 cut(s) 91, 107, 327
Bse8I GATNNNNATC 1 cut(s) 275
BseCI ATCGAT 1 cut(s) 351
BseDI CCNNGG 3 cut(s) 91, 107, 327
BseGI GGATG 4 cut(s) 18, 38, 499, 514
BseJI GATNNNNATC 1 cut(s) 275
BseRI GAGGAG 1 cut(s) 139
BseXI GCAGC 3 cut(s) 277, 383, 556
BshFI GGCC 2 cut(s) 450, 540
BshNI GGYRCC 1 cut(s) 252
BshVI ATCGAT 1 cut(s) 351
BslFI GGGAC 2 cut(s) 539, 577
BsmFI GGGAC 2 cut(s) 539, 577
BsnI GGCC 2 cut(s) 450, 540
Bsp143I GATC 3 cut(s) 133, 270, 352
Bsp19I CCATGG 1 cut(s) 107
BspACI CCGC 1 cut(s) 199
BspANI GGCC 2 cut(s) 450, 540
BspDI ATCGAT 1 cut(s) 351
BspLI GGNNCC 4 cut(s) 16, 254, 475, 486
BspT107I GGYRCC 1 cut(s) 252
BspTI CTTAAG 1 cut(s) 478
BssECI CCNNGG 3 cut(s) 91, 107, 327
BssMI GATC 3 cut(s) 133, 270, 352
BssT1I CCWWGG 3 cut(s) 91, 107, 327
Bst4CI ACNGT 1 cut(s) 505
Bst6I CTCTTC 2 cut(s) 239, 580
BstAFI CTTAAG 1 cut(s) 478
BstC8I GCNNGC 1 cut(s) 525
BstDSI CCRYGG 1 cut(s) 107
BstF5I GGATG 4 cut(s) 18, 38, 499, 514
BstKTI GATC 3 cut(s) 136, 273, 355
BstMBI GATC 3 cut(s) 133, 270, 352
BstV1I GCAGC 3 cut(s) 277, 383, 556
BstX2I RGATCY 1 cut(s) 133
BstYI RGATCY 1 cut(s) 133
Bsu15I ATCGAT 1 cut(s) 351
BsuRI GGCC 2 cut(s) 450, 540
BsuTUI ATCGAT 1 cut(s) 351
BtgI CCRYGG 1 cut(s) 107
BtsCI GGATG 4 cut(s) 18, 38, 499, 514
Cac8I GCNNGC 1 cut(s) 525
Cfr13I GGNCC 1 cut(s) 539
ClaI ATCGAT 1 cut(s) 351
CviAII CATG 4 cut(s) 108, 202, 294, 470
DpnI GATC 3 cut(s) 135, 272, 354
DpnII GATC 3 cut(s) 133, 270, 352
Eam1104I CTCTTC 2 cut(s) 239, 580
EarI CTCTTC 2 cut(s) 239, 580
Eco130I CCWWGG 3 cut(s) 91, 107, 327
Eco32I GATATC 1 cut(s) 55
EcoRV GATATC 1 cut(s) 55
EcoT14I CCWWGG 3 cut(s) 91, 107, 327
ErhI CCWWGG 3 cut(s) 91, 107, 327
FaeI CATG 4 cut(s) 111, 205, 297, 473
FalI AAGNNNNNCTT 2 cut(s) 507, 539
FaqI GGGAC 2 cut(s) 539, 577
FatI CATG 4 cut(s) 107, 201, 293, 469
Fnu4HI GCNGC 3 cut(s) 291, 372, 570
FokI GGATG 4 cut(s) 5, 25, 506, 521
Fsp4HI GCNGC 3 cut(s) 291, 372, 570
FspBI CTAG 1 cut(s) 50
GluI GCNGC 3 cut(s) 291, 372, 570
GsuI CTGGAG 1 cut(s) 134
HaeIII GGCC 2 cut(s) 450, 540
Hin1II CATG 4 cut(s) 111, 205, 297, 473
HinfI GANTC 3 cut(s) 121, 174, 360
Hpy188III TCNNGA 4 cut(s) 22, 178, 283, 499
HpyAV CCTTC 4 cut(s) 163, 202, 254, 512
HpyCH4III ACNGT 1 cut(s) 505
HpyCH4IV ACGT 2 cut(s) 31, 186
HpyCH4V TGCA 2 cut(s) 293, 527
HpySE526I ACGT 2 cut(s) 31, 186
Hsp92II CATG 4 cut(s) 111, 205, 297, 473
Kzo9I GATC 3 cut(s) 133, 270, 352
LmnI GCTCC 3 cut(s) 132, 473, 484
LpnPI CCDG 6 cut(s) 98, 163, 407, 501, 516, 555
Lsp1109I GCAGC 3 cut(s) 277, 383, 556
LweI GCATC 1 cut(s) 536
MaeI CTAG 1 cut(s) 50
MaeII ACGT 2 cut(s) 31, 186
MaeIII GTNAC 2 cut(s) 280, 385
MalI GATC 3 cut(s) 135, 272, 354
MboI GATC 3 cut(s) 133, 270, 352
MboII GAAGA 6 cut(s) 187, 226, 265, 527, 567, 570
MflI RGATCY 1 cut(s) 133
MluCI AATT 1 cut(s) 83
MnlI CCTC 7 cut(s) 52, 153, 157, 160, 484, 567, 583
MseI TTAA 1 cut(s) 479
MslI CAYNNNNRTG 1 cut(s) 431
MspCI CTTAAG 1 cut(s) 478
NcoI CCATGG 1 cut(s) 107
NdeII GATC 3 cut(s) 133, 270, 352
NlaIII CATG 4 cut(s) 111, 205, 297, 473
NlaIV GGNNCC 4 cut(s) 16, 254, 475, 486
NmuCI GTSAC 2 cut(s) 280, 385
PfeI GAWTC 3 cut(s) 121, 174, 360
PkrI GCNGC 3 cut(s) 292, 373, 571
PsiI TTATAA 1 cut(s) 431
Psp1406I AACGTT 1 cut(s) 31
PspN4I GGNNCC 4 cut(s) 16, 254, 475, 486
PspPI GGNCC 1 cut(s) 539
PsuI RGATCY 1 cut(s) 133
RseI CAYNNNNRTG 1 cut(s) 431
SaqAI TTAA 1 cut(s) 479
SatI GCNGC 3 cut(s) 291, 372, 570
Sau3AI GATC 3 cut(s) 133, 270, 352
Sau96I GGNCC 1 cut(s) 539
SetI ASST 8 cut(s) 11, 34, 51, 129, 145, 189, 376, 535
SfaNI GCATC 1 cut(s) 536
SmiMI CAYNNNNRTG 1 cut(s) 431
SmlI CTYRAG 1 cut(s) 478
SmoI CTYRAG 1 cut(s) 478
Sse9I AATT 1 cut(s) 83
SsiI CCGC 1 cut(s) 199
SspMI CTAG 1 cut(s) 50
StyI CCWWGG 3 cut(s) 91, 107, 327
TaaI ACNGT 1 cut(s) 505
TaiI ACGT 2 cut(s) 34, 189
TaqI TCGA 2 cut(s) 44, 351
TasI AATT 1 cut(s) 83
TfiI GAWTC 3 cut(s) 121, 174, 360
Tru1I TTAA 1 cut(s) 479
Tru9I TTAA 1 cut(s) 479
TseFI GTSAC 2 cut(s) 280, 385
TseI GCWGC 3 cut(s) 290, 371, 569
Tsp45I GTSAC 2 cut(s) 280, 385
TspDTI ATGAA 6 cut(s) 218, 226, 265, 360, 458, 528
TspGWI ACGGA 1 cut(s) 27
Vha464I CTTAAG 1 cut(s) 478
XspI CTAG 1 cut(s) 50
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.