Rorug02G0167200

Pollen-specific protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0012665
Physical Location & Seq
Forward (+)
14998955 .. 15003857
4903 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0167200.1

Sequence Viewer

Length: 495 bp
ATGGCTTCTGCGCTTGCTGGTGGAGCAGTGTTAGGGACAGTATTTTCAGAGCTGTACAAGGGCGTTCAGACACTGATAAGAACATCTAAGCATTTTAAAGACCTTGCCAAAAGCATCAAATTGAAATTAGAATGCTTGCTACCACTGATCAAGCAGATTGAGGAACAAAACATGGAACTTGGTCGCTCGAACCAGTGTACAGAAAAATTTAAAATAGAAATCGTCGAGGGCCTAGAGCTTGTCCAAAATTGCTCTAAGGTTGGTCCATTGGAAATTTCCAAACAGAGGAATTACATCAGAGAACTTCGTGAGTTGGATGGTTCACTTAAGAACTGGTTAGATATACTCATGTCGGATGGAGTGAGAGATGGGAAGGAGACCTTGCAATCGGTGAGGACGATGACTAAAATGATGATGGCCATGTTTATTGTTGTTCTGGGGGTTCTTGTCACAACTATCATTGTTATAATAAAGAGAAAACAAAAGGCGTTGTGA

Protein Analysis

164

Amino Acids

18.47

Weight (kDa)

9.4

Isoelectric Point (pI)

40.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 5 - 136 1e-20 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 467
AcoI YGGCCR 1 cut(s) 417
AcsI RAATTY 2 cut(s) 206, 273
AfaI GTAC 2 cut(s) 56, 199
AfiI CCNNNNNNNGG 1 cut(s) 285
AflII CTTAAG 1 cut(s) 326
AgsI TTSAA 1 cut(s) 124
AluBI AGCT 2 cut(s) 52, 238
AluI AGCT 2 cut(s) 52, 238
Alw26I GTCTC 1 cut(s) 371
AlwNI CAGNNNCTG 1 cut(s) 73
AoxI GGCC 2 cut(s) 229, 417
ApoI RAATTY 2 cut(s) 206, 273
AspLEI GCGC 1 cut(s) 13
AspS9I GGNCC 2 cut(s) 229, 263
AsuHPI GGTGA 1 cut(s) 403
AvaII GGWCC 1 cut(s) 263
BalI TGGCCA 1 cut(s) 419
BccI CCATC 4 cut(s) 311, 350, 362, 409
BclI TGATCA 1 cut(s) 147
BcoDI GTCTC 1 cut(s) 371
BfaI CTAG 1 cut(s) 233
BfrI CTTAAG 1 cut(s) 326
Bme18I GGWCC 1 cut(s) 263
BmgT120I GGNCC 2 cut(s) 229, 263
BmsI GCATC 1 cut(s) 123
BsaI GGTCTC 1 cut(s) 371
Bsc4I CCNNNNNNNGG 1 cut(s) 285
Bse1I ACTGG 2 cut(s) 193, 338
BseGI GGATG 2 cut(s) 322, 361
BseLI CCNNNNNNNGG 1 cut(s) 285
BseNI ACTGG 2 cut(s) 193, 338
BshFI GGCC 2 cut(s) 231, 419
BslFI GGGAC 1 cut(s) 49
BslI CCNNNNNNNGG 1 cut(s) 285
BsmAI GTCTC 1 cut(s) 371
BsmFI GGGAC 1 cut(s) 49
BsmI GAATGC 1 cut(s) 137
BsnI GGCC 2 cut(s) 231, 419
Bso31I GGTCTC 1 cut(s) 371
Bsp1407I TGTACA 2 cut(s) 54, 197
Bsp143I GATC 1 cut(s) 147
BspANI GGCC 2 cut(s) 231, 419
BspTI CTTAAG 1 cut(s) 326
BspTNI GGTCTC 1 cut(s) 371
BsrGI TGTACA 2 cut(s) 54, 197
BsrI ACTGG 2 cut(s) 193, 338
BssMI GATC 1 cut(s) 147
Bst4CI ACNGT 1 cut(s) 40
BstAFI CTTAAG 1 cut(s) 326
BstAUI TGTACA 2 cut(s) 54, 197
BstC8I GCNNGC 2 cut(s) 15, 137
BstDEI CTNAG 2 cut(s) 87, 255
BstF5I GGATG 2 cut(s) 322, 361
BstHHI GCGC 1 cut(s) 13
BstKTI GATC 1 cut(s) 150
BstMAI GTCTC 1 cut(s) 371
BstMBI GATC 1 cut(s) 147
BstMWI GCNNNNNNNGC 1 cut(s) 23
BsuRI GGCC 2 cut(s) 231, 419
BtsCI GGATG 2 cut(s) 322, 361
BtsI GCAGTG 1 cut(s) 33
BtsIMutI CAGTG 4 cut(s) 33, 71, 143, 200
Cac8I GCNNGC 2 cut(s) 15, 137
CaiI CAGNNNCTG 1 cut(s) 73
CfoI GCGC 1 cut(s) 13
Cfr13I GGNCC 2 cut(s) 229, 263
Csp6I GTAC 2 cut(s) 55, 198
CviAII CATG 3 cut(s) 172, 349, 421
CviJI RGCY 5 cut(s) 5, 52, 231, 238, 419
CviKI_1 RGCY 5 cut(s) 5, 52, 231, 238, 419
CviQI GTAC 2 cut(s) 55, 198
DdeI CTNAG 2 cut(s) 87, 255
DpnI GATC 1 cut(s) 149
DpnII GATC 1 cut(s) 147
DraI TTTAAA 2 cut(s) 97, 211
EaeI YGGCCR 1 cut(s) 417
Eco31I GGTCTC 1 cut(s) 371
Eco47I GGWCC 1 cut(s) 263
EcoO109I RGGNCCY 1 cut(s) 229
FaeI CATG 3 cut(s) 175, 352, 424
FaiI YATR 5 cut(s) 173, 344, 350, 422, 467
FalI AAGNNNNNCTT 2 cut(s) 365, 397
FaqI GGGAC 1 cut(s) 49
FatI CATG 3 cut(s) 171, 348, 420
FbaI TGATCA 1 cut(s) 147
FokI GGATG 2 cut(s) 329, 368
FspBI CTAG 1 cut(s) 233
GlaI GCGC 1 cut(s) 12
HaeIII GGCC 2 cut(s) 231, 419
HhaI GCGC 1 cut(s) 13
Hin1II CATG 3 cut(s) 175, 352, 424
Hin6I GCGC 1 cut(s) 11
HinP1I GCGC 1 cut(s) 11
HphI GGTGA 1 cut(s) 403
Hpy166II GTNNAC 2 cut(s) 198, 323
Hpy188I TCNGA 4 cut(s) 49, 69, 299, 355
Hpy188III TCNNGA 1 cut(s) 308
Hpy8I GTNNAC 2 cut(s) 198, 323
Hpy99I CGWCG 1 cut(s) 227
HpyAV CCTTC 1 cut(s) 367
HpyCH4III ACNGT 1 cut(s) 40
HpyCH4V TGCA 1 cut(s) 385
HpyF10VI GCNNNNNNNGC 1 cut(s) 23
HpyF3I CTNAG 2 cut(s) 87, 255
Hsp92II CATG 3 cut(s) 175, 352, 424
HspAI GCGC 1 cut(s) 11
Ksp22I TGATCA 1 cut(s) 147
Kzo9I GATC 1 cut(s) 147
LmnI GCTCC 1 cut(s) 23
LpnPI CCDG 4 cut(s) 3, 206, 319, 422
LweI GCATC 1 cut(s) 123
MaeI CTAG 1 cut(s) 233
MaeIII GTNAC 1 cut(s) 448
MalI GATC 1 cut(s) 149
MboI GATC 1 cut(s) 147
MlsI TGGCCA 1 cut(s) 419
MluCI AATT 6 cut(s) 119, 125, 206, 247, 273, 289
MluNI TGGCCA 1 cut(s) 419
MmeI TCCRAC 2 cut(s) 294, 333
MnlI CCTC 4 cut(s) 154, 220, 279, 387
Mox20I TGGCCA 1 cut(s) 419
MscI TGGCCA 1 cut(s) 419
MseI TTAA 3 cut(s) 96, 210, 327
Msp20I TGGCCA 1 cut(s) 419
MspCI CTTAAG 1 cut(s) 326
Mva1269I GAATGC 1 cut(s) 137
MwoI GCNNNNNNNGC 1 cut(s) 23
NdeII GATC 1 cut(s) 147
NlaIII CATG 3 cut(s) 175, 352, 424
NmuCI GTSAC 1 cut(s) 448
PcsI WCGNNNNNNNCGW 1 cut(s) 395
PctI GAATGC 1 cut(s) 137
PsiI TTATAA 1 cut(s) 467
PspPI GGNCC 2 cut(s) 229, 263
PstNI CAGNNNCTG 1 cut(s) 73
RsaI GTAC 2 cut(s) 56, 199
RsaNI GTAC 2 cut(s) 55, 198
SaqAI TTAA 3 cut(s) 96, 210, 327
Sau3AI GATC 1 cut(s) 147
Sau96I GGNCC 2 cut(s) 229, 263
SetI ASST 5 cut(s) 54, 105, 240, 261, 383
SfaNI GCATC 1 cut(s) 123
SinI GGWCC 1 cut(s) 263
SmlI CTYRAG 1 cut(s) 326
SmoI CTYRAG 1 cut(s) 326
Sse9I AATT 6 cut(s) 119, 125, 206, 247, 273, 289
SspMI CTAG 1 cut(s) 233
TaaI ACNGT 1 cut(s) 40
TaqI TCGA 2 cut(s) 188, 225
TasI AATT 6 cut(s) 119, 125, 206, 247, 273, 289
TatI WGTACW 2 cut(s) 54, 197
Tru1I TTAA 3 cut(s) 96, 210, 327
Tru9I TTAA 3 cut(s) 96, 210, 327
TscAI CASTG 4 cut(s) 33, 78, 150, 200
TseFI GTSAC 1 cut(s) 448
Tsp45I GTSAC 1 cut(s) 448
TspRI CASTG 4 cut(s) 33, 78, 150, 200
Vha464I CTTAAG 1 cut(s) 326
VpaK11BI GGWCC 1 cut(s) 263
XapI RAATTY 2 cut(s) 206, 273
XspI CTAG 1 cut(s) 233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.