Rorug02G0192700

Peptidyl-prolyl cis-trans isomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0009456
Physical Location & Seq
Reverse (-)
17964215 .. 17969526
5312 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0192700.1

Sequence Viewer

Length: 3054 bp
ATGGAGAGCTATTTGCAAGAGAACTTTGGGGAAGTGAAGGCTAAGGGCTCGTCTGAAGAAACGTTGCAGAAATGGAGGAAGCTCTGTGGAGTTGTGAAGAACCCGAAAAGAAGGTTTCGGTTCACTGCTAATCTTTCCAAGCGTATAGAGGCTGCGGCTATGCGCCGTACCAATCAGGAGAAATTGAGGATTGCAGTTTTGGTTTCTAAAGCTGCATTTCAATTTATTCAAGGTGTACAGCCAAGTGACTATGTTCTACCACAGGAAGTTAAAGCGGCAGGTTTTGGTATCTGTGCTGATGAATTGGGATCCATTGTGGAAGGCCATGATGTAAAGAAGCTAAAATTTCATGGAGGGGTAACTGGCCTTTCAGAAAAGCTCTCCACATCTATAAATGATGGGCTCAATACCGAAACTGATCTGCACACTCGAAGACAAGAGATTTATGGCATTAATAAGTTCACAGAGTGCGAACAGCGTGGTTTCTTTGTATTTGTTTGGGAAGCCCTTCAGGACATGACTCTTATGATCCTCGGAGTGTGTGCTTTTGTGTCATTGATCGTTGGACTAGCAATGGAAGGATGGCCAGCAGGAGCTCATGATGGGCTTGGAATTGTTGCGAGTATTATGCTGGTTGTGTTTGTCACAGCAACAAGTGATTATCGGCAATCACTACAGTTCAAGGACTTGGACAAGGAGAAGAAGAAGATTGCTATGCAAGTAACAAGAAATGGATATAGGCAGAAAATGTCTATTTATGAATTACTACCTGGTGATATTGTACACCTTTCTATTGGAGACCAAGTACCTGCAGATGGTCTATTTGTTTCTGGTTTTTCTGTCTTAATTGATGAATCCAGTTTAACTGGTGAGAGTGAGCCTATTATGGTAACTCTTGAAAATCCCTTTCTTCTTTCTGGAACTAAGGTTCAAGATGGATCATGCAAGATGATGGTTACCACAGTCGGGATGAGAACTCAATGGGGCAAATTGATGGCTACACTAAGTGAAGGTGGAGATGATGAAACCCCATTGCAGGTCAAGTTGAATGGAGTGGCAACCATTATTGGTAAGATAGGACTTTTCTTTGCTGTTGTGACTTTTGCTGTTATGGTGCAAGGACTATTTAGTCGAAAACTGCAAGAAGGGACACACTGGAGCTGGAATGGAGATGATGCAATGCAATTGTTAGAGTTCTTTGCTGTTGCAGTCACGATTGTTGTTGTTGCTGTCCCTGAGGGGCTTCCACTGGCTGTGACATTGAGTCTTGCATTTGCCATGAAGAAAATGATGAATGATAAGGCGCTTGTTCGCCATCTGGCAGCTTGCGAAACTATGGGATCAGCCACGAACATTTGCAGTGACAAAACTGGGACACTGACAACCAACAAAATGACTGTTGTGAAGTCATGCATTTGCATGAATGTCAAAGATGTGAGCAACCTCAGTGATGCTTCAAGCTTGTTCTCTGATATTCCAGAATCTGTTAAGAAGGTTTTGCTGCAATCAATATTTAACAACACTGGTGGAGAAGTTGTGGTTAGCAAAGGTGGCAAGAATGAGATGTTGGGAACACCTACCGACACTGCTTTGTTGGCGTTTGGCTTGGCGCTTGGTGGAGATTTTCAAGCAGAGAGAAAAGCTTCTAAACTTGTGAAAGTTGAGCCTTTCAACTCAACAAAGAAAAGAATGGGGGTCGTGTTGGAGCTTCCTGAAGGAGGAGGTTTCAGAGCACATACTAAAGGTGCTTCAGAAATCATTTTGGCCAACTGTGACAAGGTGATCAATTCAAAGGGTGAAACTGTTCCTCTTGATGAAGCATCCATTAAGCATCTCCAAGCTACTATCAATGAATTTGCTTGTGAGGCTCTTCGAACTATCTGCCTTGGCTATATGGAATTAGAAAAAGGATTCTCTGCTGAGGATCCCATCCCAACTTCTGGATATACTTGCATAGGAATTGTTGGCATTAAAGATCCTGTTCGTCCCGGTGTCAAGGAGTCTGTTGCAGTTTGCCGTTCAGCTGGTATCACAGTAAGAATGGTTACAGGAGACAATATTAACACTGCAAAGGCCATAGCAAGGGAATGTGGGATTCTCACTGATGATGGATTAGCCATTGAAGGTCCGGAGTTCAGGGAGAAGAATCAAGAAGAATTGCTTTCACTGATACCTAAAATTCAGGTTATGGCGCGATCATCGCCTCTAGACAAGCATACCCTGGTAAAGCACTTGAGGACCACATTTGATGAAGTCGTTGCTGTAACTGGTGATGGAACAAATGATGCCCCAGCACTTCATGAGGCAGATATTGGACTAGCCATGGGCATTGCCGGAACTGAGGTGGCCAAGGAGAGTGCTGATGTTATAATTCTGGATGATAATTTCTCCACAATTGTGACGGTGGCAAAATGGGGACGTTCGGTTTACATCAATATCCAAAAATTTGTGCAGTTCCAGCTGACTGTTAATATTGTTGCATTGATTGTTAACTTCTCCTCGGCCTGTTTGACAGGGAGTGCTCCCCTCACTGCTGTTCAGCTATTGTGGGTGAACATGATTATGGACACACTGGGAGCACTTGCACTTGCAACTGAGCCTCCAAATAATGACTTAATGAAGCGTCCGCCAGTTGGAAAGAGACAAAATTTCATCAGTAATGTAATGTGGAGGAATATTTTAGGGCAGTCTTTCTATCAATTTACAGTGTTATGGCTTCTTCAGGCTAAAGGAGAATCAATATTTCGTCTTAATGGACCAGATTCTAACTTGGTCCTGAACACACTCATTTTCAATACATTCGTCTTCTGTCAGGTGTTTAACGAAATAAGCTCGCGTGAGATGGAAGAAATAGATGTTTTCAAGGGCATATTGGATAATTACGTTTTCGTAGCAGTCCTCAGTGCCACAGTTGTGTTCCAAATCATTATTATCGAGTTTCTGGGGAAGTTTGCTAGCACAGCACCTCTCACCTTTGTTCAATGGTTTGTTACTGTGTTCATTGGCTTTTTAGGGATGCCAATCGCTGCTGGTTTGAAGAAGATTCCTGTTTGA

Protein Analysis

1017

Amino Acids

110.79

Weight (kDa)

5.92

Isoelectric Point (pI)

34.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CaATP_NAI PF12515 5 - 49 6.3e-21 Ca2+-ATPase N terminal autoinhibitory domain
Cation_ATPase_N PF00690 117 - 185 3.8e-09 Cation transporter/ATPase, N-terminus
E1-E2_ATPase PF00122 238 - 334 4.2e-21 P-type ATPase actuator domain
Cation_ATPase PF13246 515 - 663 4e-33 P-type ATPase, cytoplasmic domain N
Hydrolase PF00702 636 - 770 2.3e-11 haloacid dehalogenase-like hydrolase
Cation_ATPase_C PF00689 841 - 1014 3.3e-42 Cation transporting ATPase, C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2369
AasI GACNNNNNNGTC 1 cut(s) 1128
Acc36I ACCTGC 3 cut(s) 269, 817, 1027
AccB7I CCANNNNNTGG 1 cut(s) 1940
AccII CGCG 2 cut(s) 2194, 2836
AccIII TCCGGA 1 cut(s) 2128
AciI CCGC 3 cut(s) 155, 275, 2627
AclI AACGTT 1 cut(s) 62
AclWI GGATC 8 cut(s) 303, 316, 523, 946, 1348, 1919, 1932, 1970
AcoI YGGCCR 3 cut(s) 584, 1764, 2346
AcsI RAATTY 5 cut(s) 344, 1853, 2178, 2444, 2647
AcuI CTGAAG 5 cut(s) 75, 494, 1734, 1734, 2705
AdeI CACNNNGTG 2 cut(s) 468, 1007
AfaI GTAC 4 cut(s) 169, 237, 783, 807
AfiI CCNNNNNNNGG 7 cut(s) 815, 966, 1036, 1718, 1940, 2082, 2633
AhdI GACNNNNNGTC 1 cut(s) 1263
AjnI CCWGG 2 cut(s) 769, 2220
AjuI GAANNNNNNNTTGG 2 cut(s) 1550, 1582
AleI CACNNNNGTG 2 cut(s) 2396, 2912
AloI GAACNNNNNNTCC 2 cut(s) 912, 944
Alw21I GWGCWC 4 cut(s) 598, 1735, 2524, 2581
Alw26I GTCTC 3 cut(s) 792, 2046, 2635
AlwI GGATC 8 cut(s) 303, 316, 523, 946, 1348, 1919, 1932, 1970
AlwNI CAGNNNCTG 1 cut(s) 1484
Aor13HI TCCGGA 1 cut(s) 2128
AoxI GGCC 7 cut(s) 322, 364, 584, 1764, 2073, 2346, 2502
ApeKI GCWGC 5 cut(s) 152, 212, 1322, 1501, 3026
ApoI RAATTY 5 cut(s) 344, 1853, 2178, 2444, 2647
AseI ATTAAT 1 cut(s) 453
Asp700I GAANNNNTTC 3 cut(s) 507, 1642, 1803
AspLEI GCGC 4 cut(s) 165, 1306, 1612, 2194
AspS9I GGNCC 4 cut(s) 2126, 2238, 2756, 2773
AsuC2I CCSGG 1 cut(s) 1989
AsuHPI GGTGA 7 cut(s) 785, 881, 1792, 1808, 2282, 2563, 2962
AsuII TTCGAA 1 cut(s) 1873
AsuNHI GCTAGC 1 cut(s) 2954
AvaII GGWCC 4 cut(s) 2126, 2238, 2756, 2773
AxyI CCTNAGG 1 cut(s) 1236
BalI TGGCCA 3 cut(s) 586, 1766, 2348
BamHI GGATCC 2 cut(s) 308, 1924
BanII GRGCYC 3 cut(s) 50, 405, 598
BbsI GAAGAC 2 cut(s) 439, 2797
Bbv12I GWGCWC 4 cut(s) 598, 1735, 2524, 2581
BbvCI CCTCAGC 1 cut(s) 1920
BbvI GCAGC 5 cut(s) 139, 199, 1334, 1488, 3013
BceAI ACGGC 2 cut(s) 150, 2001
BcgI CGANNNNNNTGC 4 cut(s) 610, 644, 1863, 1897
BciT130I CCWGG 2 cut(s) 771, 2222
BclI TGATCA 1 cut(s) 1782
BcnI CCSGG 1 cut(s) 1989
BcoDI GTCTC 3 cut(s) 792, 2046, 2635
BfaI CTAG 4 cut(s) 569, 2207, 2318, 2955
BfmI CTRYAG 2 cut(s) 674, 810
BfoI RGCGCY 2 cut(s) 1307, 1613
BfuAI ACCTGC 3 cut(s) 269, 817, 1027
BisI GCNGC 7 cut(s) 153, 156, 213, 276, 1323, 1502, 3027
BlsI GCNGC 7 cut(s) 154, 157, 214, 277, 1324, 1503, 3028
Bme1390I CCNGG 3 cut(s) 771, 1989, 2222
Bme18I GGWCC 4 cut(s) 2126, 2238, 2756, 2773
BmeRI GACNNNNNGTC 1 cut(s) 1263
BmgT120I GGNCC 4 cut(s) 2126, 2238, 2756, 2773
BmiI GGNNCC 2 cut(s) 310, 1926
BmrFI CCNGG 3 cut(s) 771, 1989, 2222
BmrI ACTGGG 2 cut(s) 1380, 2582
BmsI GCATC 6 cut(s) 1165, 1441, 1829, 1840, 2275, 3006
BmtI GCTAGC 1 cut(s) 2958
BmuI ACTGGG 2 cut(s) 1380, 2582
BpiI GAAGAC 2 cut(s) 439, 2797
BpmI CTGGAG 1 cut(s) 1177
Bpu10I CCTNAGC 2 cut(s) 42, 1920
Bpu14I TTCGAA 1 cut(s) 1873
BpuEI CTTGAG 1 cut(s) 2254
BpuMI CCSGG 1 cut(s) 1989
BsaBI GATNNNNATC 1 cut(s) 3020
BsaI GGTCTC 1 cut(s) 792
BsaJI CCNNGG 6 cut(s) 532, 1885, 2220, 2322, 2349, 2499
BsaWI WCCGGW 1 cut(s) 2128
BsaXI ACNNNNNCTCC 6 cut(s) 789, 819, 1521, 1551, 2584, 2614
Bsc4I CCNNNNNNNGG 7 cut(s) 815, 966, 1036, 1718, 1940, 2082, 2633
Bse21I CCTNAGG 1 cut(s) 1236
Bse3DI GCAATG 4 cut(s) 579, 1031, 1185, 2328
Bse8I GATNNNNATC 1 cut(s) 3020
BseAI TCCGGA 1 cut(s) 2128
BseBI CCWGG 2 cut(s) 771, 2222
BseDI CCNNGG 6 cut(s) 532, 1885, 2220, 2322, 2349, 2499
BseGI GGATG 6 cut(s) 587, 975, 1820, 1929, 2383, 3021
BseJI GATNNNNATC 1 cut(s) 3020
BseLI CCNNNNNNNGG 7 cut(s) 815, 966, 1036, 1718, 1940, 2082, 2633
BseMI GCAATG 4 cut(s) 579, 1031, 1185, 2328
BseMII CTCAG 6 cut(s) 1227, 1459, 1911, 2331, 2586, 2914
BseRI GAGGAG 2 cut(s) 1734, 2488
BseXI GCAGC 5 cut(s) 139, 199, 1334, 1488, 3013
BseYI CCCAGC 1 cut(s) 2290
BsgI GTGCAG 2 cut(s) 407, 2471
Bsh1236I CGCG 2 cut(s) 2194, 2836
BshFI GGCC 7 cut(s) 324, 366, 586, 1766, 2075, 2348, 2504
BsiHKAI GWGCWC 4 cut(s) 598, 1735, 2524, 2581
BsiSI CCGG 3 cut(s) 1989, 2129, 2334
BslFI GGGAC 5 cut(s) 1162, 1217, 1387, 1971, 2430
BslI CCNNNNNNNGG 7 cut(s) 815, 966, 1036, 1718, 1940, 2082, 2633
BsmAI GTCTC 3 cut(s) 792, 2046, 2635
BsmFI GGGAC 5 cut(s) 1162, 1217, 1387, 1971, 2430
BsnI GGCC 7 cut(s) 324, 366, 586, 1766, 2075, 2348, 2504
Bso31I GGTCTC 1 cut(s) 792
Bsp119I TTCGAA 1 cut(s) 1873
Bsp1286I GDGCHC 6 cut(s) 50, 405, 598, 1735, 2524, 2581
Bsp13I TCCGGA 1 cut(s) 2128
Bsp1407I TGTACA 2 cut(s) 235, 781
Bsp19I CCATGG 1 cut(s) 2322
BspACI CCGC 3 cut(s) 155, 275, 2627
BspANI GGCC 7 cut(s) 324, 366, 586, 1766, 2075, 2348, 2504
BspCNI CTCAG 6 cut(s) 1228, 1458, 1912, 2332, 2587, 2913
BspEI TCCGGA 1 cut(s) 2128
BspFNI CGCG 2 cut(s) 2194, 2836
BspHI TCATGA 2 cut(s) 598, 2299
BspLI GGNNCC 2 cut(s) 310, 1926
BspMAI CTGCAG 1 cut(s) 814
BspMI ACCTGC 3 cut(s) 269, 817, 1027
BspOI GCTAGC 1 cut(s) 2958
BspPI GGATC 8 cut(s) 303, 316, 523, 946, 1348, 1919, 1932, 1970
BspQI GCTCTTC 1 cut(s) 1875
BspT104I TTCGAA 1 cut(s) 1873
BspTNI GGTCTC 1 cut(s) 792
BsrDI GCAATG 4 cut(s) 579, 1031, 1185, 2328
BsrGI TGTACA 2 cut(s) 235, 781
BssECI CCNNGG 6 cut(s) 532, 1885, 2220, 2322, 2349, 2499
BssT1I CCWWGG 3 cut(s) 1885, 2322, 2349
Bst2UI CCWGG 2 cut(s) 771, 2222
Bst6I CTCTTC 1 cut(s) 1875
BstAUI TGTACA 2 cut(s) 235, 781
BstBI TTCGAA 1 cut(s) 1873
BstC8I GCNNGC 4 cut(s) 588, 1327, 2834, 2956
BstDEI CTNAG 9 cut(s) 42, 924, 1004, 1236, 1445, 1920, 2340, 2595, 2900
BstDSI CCRYGG 1 cut(s) 2322
BstEII GGTNACC 1 cut(s) 955
BstENI CCTNNNNNAGG 1 cut(s) 1716
BstF5I GGATG 6 cut(s) 587, 975, 1820, 1929, 2383, 3021
BstFNI CGCG 2 cut(s) 2194, 2836
BstH2I RGCGCY 2 cut(s) 1307, 1613
BstHHI GCGC 4 cut(s) 165, 1306, 1612, 2194
BstMAI GTCTC 3 cut(s) 792, 2046, 2635
BstMWI GCNNNNNNNGC 6 cut(s) 1551, 1595, 1865, 2200, 2458, 2960
BstNI CCWGG 2 cut(s) 771, 2222
BstPI GGTNACC 1 cut(s) 955
BstSCI CCNGG 3 cut(s) 769, 1987, 2220
BstSFI CTRYAG 2 cut(s) 674, 810
BstUI CGCG 2 cut(s) 2194, 2836
BstV1I GCAGC 5 cut(s) 139, 199, 1334, 1488, 3013
BstV2I GAAGAC 2 cut(s) 439, 2797
BstX2I RGATCY 3 cut(s) 308, 1924, 1975
BstYI RGATCY 3 cut(s) 308, 1924, 1975
Bsu36I CCTNAGG 1 cut(s) 1236
BsuRI GGCC 7 cut(s) 324, 366, 586, 1766, 2075, 2348, 2504
BtgI CCRYGG 1 cut(s) 2322
BtgZI GCGATG 1 cut(s) 2184
BtsCI GGATG 6 cut(s) 587, 975, 1820, 1929, 2383, 3021
BtsI GCAGTG 5 cut(s) 123, 1366, 1584, 2064, 2529
BveI ACCTGC 3 cut(s) 269, 817, 1027
Cac8I GCNNGC 4 cut(s) 588, 1327, 2834, 2956
CaiI CAGNNNCTG 1 cut(s) 1484
CciI TCATGA 2 cut(s) 598, 2299
CfoI GCGC 4 cut(s) 165, 1306, 1612, 2194
Cfr13I GGNCC 4 cut(s) 2126, 2238, 2756, 2773
CseI GACGC 1 cut(s) 2612
CsiI ACCWGGT 1 cut(s) 769
Csp6I GTAC 4 cut(s) 168, 236, 782, 806
CspCI CAANNNNNGTGG 2 cut(s) 1507, 1542
CviQI GTAC 4 cut(s) 168, 236, 782, 806
DdeI CTNAG 9 cut(s) 42, 924, 1004, 1236, 1445, 1920, 2340, 2595, 2900
DraIII CACNNNGTG 2 cut(s) 468, 1007
DrdI GACNNNNNNGTC 1 cut(s) 1128
DriI GACNNNNNGTC 1 cut(s) 1263
DseDI GACNNNNNNGTC 1 cut(s) 1128
EaeI YGGCCR 3 cut(s) 584, 1764, 2346
Eam1104I CTCTTC 1 cut(s) 1875
Eam1105I GACNNNNNGTC 1 cut(s) 1263
EarI CTCTTC 1 cut(s) 1875
EciI GGCGGA 1 cut(s) 2616
Ecl136II GAGCTC 1 cut(s) 596
Eco130I CCWWGG 3 cut(s) 1885, 2322, 2349
Eco24I GRGCYC 3 cut(s) 50, 405, 598
Eco31I GGTCTC 1 cut(s) 792
Eco47I GGWCC 4 cut(s) 2126, 2238, 2756, 2773
Eco53kI GAGCTC 1 cut(s) 596
Eco57I CTGAAG 5 cut(s) 75, 494, 1734, 1734, 2705
Eco81I CCTNAGG 1 cut(s) 1236
Eco91I GGTNACC 1 cut(s) 955
EcoICRI GAGCTC 1 cut(s) 596
EcoNI CCTNNNNNAGG 1 cut(s) 1716
EcoO65I GGTNACC 1 cut(s) 955
EcoRII CCWGG 2 cut(s) 769, 2220
EcoT14I CCWWGG 3 cut(s) 1885, 2322, 2349
EcoT22I ATGCAT 1 cut(s) 1415
EcoT38I GRGCYC 3 cut(s) 50, 405, 598
ErhI CCWWGG 3 cut(s) 1885, 2322, 2349
FalI AAGNNNNNCTT 2 cut(s) 2145, 2177
FaqI GGGAC 5 cut(s) 1162, 1217, 1387, 1971, 2430
FbaI TGATCA 1 cut(s) 1782
Fnu4HI GCNGC 7 cut(s) 153, 156, 213, 276, 1323, 1502, 3027
FokI GGATG 6 cut(s) 594, 982, 1807, 1916, 2390, 3028
FriOI GRGCYC 3 cut(s) 50, 405, 598
Fsp4HI GCNGC 7 cut(s) 153, 156, 213, 276, 1323, 1502, 3027
FspBI CTAG 4 cut(s) 569, 2207, 2318, 2955
GlaI GCGC 4 cut(s) 164, 1305, 1611, 2193
GluI GCNGC 7 cut(s) 153, 156, 213, 276, 1323, 1502, 3027
GsaI CCCAGC 1 cut(s) 2294
GsuI CTGGAG 1 cut(s) 1177
HaeII RGCGCY 2 cut(s) 1307, 1613
HaeIII GGCC 7 cut(s) 324, 366, 586, 1766, 2075, 2348, 2504
HapII CCGG 3 cut(s) 1989, 2129, 2334
HgaI GACGC 1 cut(s) 2612
HhaI GCGC 4 cut(s) 165, 1306, 1612, 2194
Hin6I GCGC 4 cut(s) 163, 1304, 1610, 2192
HinP1I GCGC 4 cut(s) 163, 1304, 1610, 2192
HincII GTYRAC 1 cut(s) 2491
HindII GTYRAC 1 cut(s) 2491
HindIII AAGCTT 2 cut(s) 1459, 1641
HpaI GTTAAC 1 cut(s) 2491
HpaII CCGG 3 cut(s) 1989, 2129, 2334
HphI GGTGA 7 cut(s) 785, 881, 1792, 1808, 2282, 2563, 2962
Hpy166II GTNNAC 7 cut(s) 123, 236, 462, 784, 2428, 2491, 2554
Hpy188I TCNGA 6 cut(s) 55, 373, 536, 1471, 1730, 1753
Hpy8I GTNNAC 7 cut(s) 123, 236, 462, 784, 2428, 2491, 2554
HpyCH4IV ACGT 3 cut(s) 62, 2419, 2883
HpyF10VI GCNNNNNNNGC 6 cut(s) 1551, 1595, 1865, 2200, 2458, 2960
HpyF3I CTNAG 9 cut(s) 42, 924, 1004, 1236, 1445, 1920, 2340, 2595, 2900
HpySE526I ACGT 3 cut(s) 62, 2419, 2883
HspAI GCGC 4 cut(s) 163, 1304, 1610, 2192
Kpn2I TCCGGA 1 cut(s) 2128
Ksp22I TGATCA 1 cut(s) 1782
KspAI GTTAAC 1 cut(s) 2491
LguI GCTCTTC 1 cut(s) 1875
LmnI GCTCC 5 cut(s) 593, 1158, 1705, 2527, 2576
Lsp1109I GCAGC 5 cut(s) 139, 199, 1334, 1488, 3013
LweI GCATC 6 cut(s) 1165, 1441, 1829, 1840, 2275, 3006
MabI ACCWGGT 1 cut(s) 769
MaeI CTAG 4 cut(s) 569, 2207, 2318, 2955
MaeII ACGT 3 cut(s) 62, 2419, 2883
MfeI CAATTG 2 cut(s) 1184, 2394
MflI RGATCY 3 cut(s) 308, 1924, 1975
MhlI GDGCHC 6 cut(s) 50, 405, 598, 1735, 2524, 2581
MlsI TGGCCA 3 cut(s) 586, 1766, 2348
MluNI TGGCCA 3 cut(s) 586, 1766, 2348
MlyI GAGTC 3 cut(s) 514, 1273, 2009
MmeI TCCRAC 3 cut(s) 544, 1683, 2614
Mox20I TGGCCA 3 cut(s) 586, 1766, 2348
Mph1103I ATGCAT 1 cut(s) 1415
MroI TCCGGA 1 cut(s) 2128
MroXI GAANNNNTTC 3 cut(s) 507, 1642, 1803
MscI TGGCCA 3 cut(s) 586, 1766, 2348
MslI CAYNNNNRTG 4 cut(s) 1418, 2396, 2561, 2912
Msp20I TGGCCA 3 cut(s) 586, 1766, 2348
MspA1I CMGCKG 2 cut(s) 2024, 2461
MspI CCGG 3 cut(s) 1989, 2129, 2334
MspR9I CCNGG 3 cut(s) 771, 1989, 2222
MunI CAATTG 2 cut(s) 1184, 2394
MvaI CCWGG 2 cut(s) 771, 2222
MvnI CGCG 2 cut(s) 2194, 2836
MwoI GCNNNNNNNGC 6 cut(s) 1551, 1595, 1865, 2200, 2458, 2960
NciI CCSGG 1 cut(s) 1989
NcoI CCATGG 1 cut(s) 2322
NheI GCTAGC 1 cut(s) 2954
NlaIV GGNNCC 2 cut(s) 310, 1926
NmeAIII GCCGAG 1 cut(s) 2480
NmuCI GTSAC 8 cut(s) 245, 643, 1096, 1210, 1255, 1361, 1772, 2398
NsiI ATGCAT 1 cut(s) 1415
NspV TTCGAA 1 cut(s) 1873
OliI CACNNNNGTG 2 cut(s) 2396, 2912
PagI TCATGA 2 cut(s) 598, 2299
PciSI GCTCTTC 1 cut(s) 1875
PdmI GAANNNNTTC 3 cut(s) 507, 1642, 1803
PfeI GAWTC 8 cut(s) 854, 1481, 1911, 2095, 2146, 2735, 2762, 3043
PflMI CCANNNNNTGG 1 cut(s) 1940
PkrI GCNGC 7 cut(s) 154, 157, 214, 277, 1324, 1503, 3028
PleI GAGTC 3 cut(s) 514, 1272, 2008
PpsI GAGTC 3 cut(s) 514, 1272, 2008
PshBI ATTAAT 1 cut(s) 453
PsiI TTATAA 1 cut(s) 2369
Psp124BI GAGCTC 1 cut(s) 598
Psp1406I AACGTT 1 cut(s) 62
Psp6I CCWGG 2 cut(s) 769, 2220
PspEI GGTNACC 1 cut(s) 955
PspFI CCCAGC 1 cut(s) 2290
PspGI CCWGG 2 cut(s) 769, 2220
PspN4I GGNNCC 2 cut(s) 310, 1926
PspPI GGNCC 4 cut(s) 2126, 2238, 2756, 2773
PstI CTGCAG 1 cut(s) 814
PstNI CAGNNNCTG 1 cut(s) 1484
PsuI RGATCY 3 cut(s) 308, 1924, 1975
PvuII CAGCTG 2 cut(s) 2024, 2461
RsaI GTAC 4 cut(s) 169, 237, 783, 807
RsaNI GTAC 4 cut(s) 168, 236, 782, 806
RseI CAYNNNNRTG 4 cut(s) 1418, 2396, 2561, 2912
SacI GAGCTC 1 cut(s) 598
SapI GCTCTTC 1 cut(s) 1875
SatI GCNGC 7 cut(s) 153, 156, 213, 276, 1323, 1502, 3027
Sau96I GGNCC 4 cut(s) 2126, 2238, 2756, 2773
SchI GAGTC 3 cut(s) 514, 1273, 2009
ScrFI CCNGG 3 cut(s) 771, 1989, 2222
SduI GDGCHC 6 cut(s) 50, 405, 598, 1735, 2524, 2581
SexAI ACCWGGT 1 cut(s) 769
SfaNI GCATC 6 cut(s) 1165, 1441, 1829, 1840, 2275, 3006
SfcI CTRYAG 2 cut(s) 674, 810
SfuI TTCGAA 1 cut(s) 1873
SinI GGWCC 4 cut(s) 2126, 2238, 2756, 2773
SmiMI CAYNNNNRTG 4 cut(s) 1418, 2396, 2561, 2912
SmlI CTYRAG 1 cut(s) 2233
SmoI CTYRAG 1 cut(s) 2233
SsiI CCGC 3 cut(s) 155, 275, 2627
SspI AATATT 5 cut(s) 1512, 2059, 2473, 2677, 2742
SspMI CTAG 4 cut(s) 569, 2207, 2318, 2955
SstI GAGCTC 1 cut(s) 598
StyD4I CCNGG 3 cut(s) 769, 1987, 2220
StyI CCWWGG 3 cut(s) 1885, 2322, 2349
TaiI ACGT 3 cut(s) 65, 2422, 2886
TaqI TCGA 4 cut(s) 430, 1132, 1873, 2934
TatI WGTACW 2 cut(s) 235, 781
TauI GCSGC 2 cut(s) 158, 278
TfiI GAWTC 8 cut(s) 854, 1481, 1911, 2095, 2146, 2735, 2762, 3043
TseFI GTSAC 8 cut(s) 245, 643, 1096, 1210, 1255, 1361, 1772, 2398
TseI GCWGC 5 cut(s) 152, 212, 1322, 1501, 3026
Tsp45I GTSAC 8 cut(s) 245, 643, 1096, 1210, 1255, 1361, 1772, 2398
Van91I CCANNNNNTGG 1 cut(s) 1940
VpaK11BI GGWCC 4 cut(s) 2126, 2238, 2756, 2773
VspI ATTAAT 1 cut(s) 453
XagI CCTNNNNNAGG 1 cut(s) 1716
XapI RAATTY 5 cut(s) 344, 1853, 2178, 2444, 2647
XbaI TCTAGA 1 cut(s) 2206
XmnI GAANNNNTTC 3 cut(s) 507, 1642, 1803
XspI CTAG 4 cut(s) 569, 2207, 2318, 2955
Zsp2I ATGCAT 1 cut(s) 1415
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.