Rorug02G0228200

zinc finger CCCH domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0005393
Physical Location & Seq
Forward (+)
22611184 .. 22612072
889 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0228200.1

Sequence Viewer

Length: 528 bp
ATGGGCTGGTCAGAATCAGAGAGAGGACTCTGCAAGAAGCACCCAGAAGTGAAGCAGTTCCCCGGCGCCGTCTGCTCGACATGTCTCAGTGAAAAGCTCTCTCAGCTCTACGCTCCCTCCATTTCCGACAGAAAACTAACCGCAGATTTCTTGGGTTACGGCTTCCTCCCAATCCACTCTTGCTCTTCTTCTAACTACTCCTCCGCTTCCTCATCACCCCACGCCTCTCCTCCGGTGATCAATCCCGGCCGTCGAGGGCACCACCAGCGCAACGCGTCGGAGGTGATGGGCCGTTCGATATCTTTCATGTTCAGCAGTGATGGGACTAAGCAGGGTGGAGGGCATGGGTTGATGAAGAAGAGCAGATCGGTTGCGGTGGTGACGAGGAGTGCTGGAAGTCATAGCTTTGGAGATCAGGTGGTGATGAGGAGTGGGAAGAAGAAACGAGGGTTTTGGTCGAAGCTGATTAGGGGGACGGGGAGGAAGACCAAGCAGGTTTTTAAGCACTCCAAGAGTGCTCTAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-CCCH

Protein Analysis

175

Amino Acids

19.02

Weight (kDa)

10.53

Isoelectric Point (pI)

52.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 484
AccB1I GGYRCC 2 cut(s) 65, 258
AccII CGCG 1 cut(s) 275
AciI CCGC 3 cut(s) 141, 204, 374
AcoI YGGCCR 1 cut(s) 247
AcyI GRCGYC 1 cut(s) 66
AflIII ACRYGT 2 cut(s) 80, 273
AluBI AGCT 4 cut(s) 97, 106, 405, 463
AluI AGCT 4 cut(s) 97, 106, 405, 463
Alw21I GWGCWC 1 cut(s) 520
Alw26I GTCTC 1 cut(s) 89
AoxI GGCC 2 cut(s) 247, 289
Asp700I GAANNNNTTC 1 cut(s) 56
AspLEI GCGC 2 cut(s) 68, 270
AspS9I GGNCC 1 cut(s) 289
AsuC2I CCSGG 2 cut(s) 63, 246
AsuHPI GGTGA 5 cut(s) 207, 247, 295, 391, 433
BaeGI GKGCMC 1 cut(s) 261
BanI GGYRCC 2 cut(s) 65, 258
BbsI GAAGAC 1 cut(s) 491
Bbv12I GWGCWC 1 cut(s) 520
BccI CCATC 2 cut(s) 280, 314
BceAI ACGGC 4 cut(s) 53, 175, 234, 276
BclI TGATCA 1 cut(s) 237
BcnI CCSGG 2 cut(s) 63, 246
BcoDI GTCTC 1 cut(s) 89
BfoI RGCGCY 1 cut(s) 69
BfuAI ACCTGC 1 cut(s) 484
Bme1390I CCNGG 2 cut(s) 63, 246
BmgT120I GGNCC 1 cut(s) 289
BmiI GGNNCC 2 cut(s) 67, 260
BmrFI CCNGG 2 cut(s) 63, 246
BpiI GAAGAC 1 cut(s) 491
BpuMI CCSGG 2 cut(s) 63, 246
BsaHI GRCGYC 1 cut(s) 66
BsaJI CCNNGG 1 cut(s) 61
BsaWI WCCGGW 1 cut(s) 232
BsaXI ACNNNNNCTCC 6 cut(s) 101, 131, 185, 215, 402, 432
BseDI CCNNGG 1 cut(s) 61
BseMII CTCAG 2 cut(s) 100, 116
BseRI GAGGAG 4 cut(s) 190, 219, 400, 442
BseSI GKGCMC 1 cut(s) 261
BseX3I CGGCCG 1 cut(s) 247
Bsh1236I CGCG 1 cut(s) 275
Bsh1285I CGRYCG 1 cut(s) 250
BshFI GGCC 2 cut(s) 249, 291
BshNI GGYRCC 2 cut(s) 65, 258
BsiEI CGRYCG 1 cut(s) 250
BsiHKAI GWGCWC 1 cut(s) 520
BsiSI CCGG 3 cut(s) 63, 233, 246
BslFI GGGAC 2 cut(s) 337, 487
BsmAI GTCTC 1 cut(s) 89
BsmFI GGGAC 2 cut(s) 337, 487
BsnI GGCC 2 cut(s) 249, 291
Bsp1286I GDGCHC 2 cut(s) 261, 520
Bsp143I GATC 3 cut(s) 237, 365, 412
BspACI CCGC 3 cut(s) 141, 204, 374
BspANI GGCC 2 cut(s) 249, 291
BspCNI CTCAG 2 cut(s) 99, 115
BspFNI CGCG 1 cut(s) 275
BspLI GGNNCC 2 cut(s) 67, 260
BspMI ACCTGC 1 cut(s) 484
BspQI GCTCTTC 2 cut(s) 190, 353
BspT107I GGYRCC 2 cut(s) 65, 258
BssECI CCNNGG 1 cut(s) 61
BssMI GATC 3 cut(s) 237, 365, 412
BssNI GRCGYC 1 cut(s) 66
Bst6I CTCTTC 2 cut(s) 190, 353
BstACI GRCGYC 1 cut(s) 66
BstDEI CTNAG 4 cut(s) 86, 102, 327, 521
BstFNI CGCG 1 cut(s) 275
BstH2I RGCGCY 1 cut(s) 69
BstHHI GCGC 2 cut(s) 68, 270
BstKTI GATC 3 cut(s) 240, 368, 415
BstMAI GTCTC 1 cut(s) 89
BstMBI GATC 3 cut(s) 237, 365, 412
BstMCI CGRYCG 1 cut(s) 250
BstMWI GCNNNNNNNGC 3 cut(s) 72, 103, 265
BstNSI RCATGY 1 cut(s) 84
BstSCI CCNGG 2 cut(s) 61, 244
BstSLI GKGCMC 1 cut(s) 261
BstUI CGCG 1 cut(s) 275
BstV2I GAAGAC 1 cut(s) 491
BstZI CGGCCG 1 cut(s) 247
BsuRI GGCC 2 cut(s) 249, 291
BtsI GCAGTG 1 cut(s) 322
BtsIMutI CAGTG 2 cut(s) 94, 322
BveI ACCTGC 1 cut(s) 484
CfoI GCGC 2 cut(s) 68, 270
Cfr13I GGNCC 1 cut(s) 289
CseI GACGC 1 cut(s) 264
CviAII CATG 3 cut(s) 81, 307, 344
CviJI RGCY 8 cut(s) 6, 97, 106, 162, 249, 291, 405, 463
CviKI_1 RGCY 8 cut(s) 6, 97, 106, 162, 249, 291, 405, 463
DdeI CTNAG 4 cut(s) 86, 102, 327, 521
DinI GGCGCC 1 cut(s) 67
DpnI GATC 3 cut(s) 239, 367, 414
DpnII GATC 3 cut(s) 237, 365, 412
EaeI YGGCCR 1 cut(s) 247
EagI CGGCCG 1 cut(s) 247
Eam1104I CTCTTC 2 cut(s) 190, 353
EarI CTCTTC 2 cut(s) 190, 353
EclXI CGGCCG 1 cut(s) 247
Eco32I GATATC 1 cut(s) 300
Eco52I CGGCCG 1 cut(s) 247
EcoRV GATATC 1 cut(s) 300
EgeI GGCGCC 1 cut(s) 67
EheI GGCGCC 1 cut(s) 67
FaeI CATG 3 cut(s) 84, 310, 347
FaiI YATR 4 cut(s) 82, 308, 345, 402
FaqI GGGAC 2 cut(s) 337, 487
FatI CATG 3 cut(s) 80, 306, 343
FbaI TGATCA 1 cut(s) 237
GlaI GCGC 2 cut(s) 67, 269
HaeII RGCGCY 1 cut(s) 69
HaeIII GGCC 2 cut(s) 249, 291
HapII CCGG 3 cut(s) 63, 233, 246
HgaI GACGC 1 cut(s) 264
HhaI GCGC 2 cut(s) 68, 270
Hin1I GRCGYC 1 cut(s) 66
Hin1II CATG 3 cut(s) 84, 310, 347
Hin6I GCGC 2 cut(s) 66, 268
HinP1I GCGC 2 cut(s) 66, 268
HinfI GANTC 2 cut(s) 14, 27
HpaII CCGG 3 cut(s) 63, 233, 246
HphI GGTGA 5 cut(s) 207, 247, 295, 391, 433
Hpy188I TCNGA 4 cut(s) 13, 19, 127, 280
Hpy99I CGWCG 2 cut(s) 255, 280
HpyCH4V TGCA 1 cut(s) 33
HpyF10VI GCNNNNNNNGC 3 cut(s) 72, 103, 265
HpyF3I CTNAG 4 cut(s) 86, 102, 327, 521
Hsp92I GRCGYC 1 cut(s) 66
Hsp92II CATG 3 cut(s) 84, 310, 347
HspAI GCGC 2 cut(s) 66, 268
KasI GGCGCC 1 cut(s) 65
Ksp22I TGATCA 1 cut(s) 237
Kzo9I GATC 3 cut(s) 237, 365, 412
LguI GCTCTTC 2 cut(s) 190, 353
LmnI GCTCC 1 cut(s) 118
LpnPI CCDG 9 cut(s) 57, 76, 246, 259, 278, 317, 378, 401, 479
MaeIII GTNAC 2 cut(s) 155, 379
MalI GATC 3 cut(s) 239, 367, 414
MboI GATC 3 cut(s) 237, 365, 412
MboII GAAGA 7 cut(s) 177, 180, 367, 370, 448, 451, 496
MhlI GDGCHC 2 cut(s) 261, 520
MluI ACGCGT 1 cut(s) 273
Mly113I GGCGCC 1 cut(s) 66
MlyI GAGTC 1 cut(s) 21
MmeI TCCRAC 2 cut(s) 150, 258
MroXI GAANNNNTTC 1 cut(s) 56
MseI TTAA 1 cut(s) 501
MspI CCGG 3 cut(s) 63, 233, 246
MspR9I CCNGG 2 cut(s) 63, 246
MvnI CGCG 1 cut(s) 275
MwoI GCNNNNNNNGC 3 cut(s) 72, 103, 265
NarI GGCGCC 1 cut(s) 66
NciI CCSGG 2 cut(s) 63, 246
NdeII GATC 3 cut(s) 237, 365, 412
NlaIII CATG 3 cut(s) 84, 310, 347
NlaIV GGNNCC 2 cut(s) 67, 260
NmuCI GTSAC 1 cut(s) 379
NspI RCATGY 1 cut(s) 84
PciI ACATGT 1 cut(s) 80
PciSI GCTCTTC 2 cut(s) 190, 353
PdmI GAANNNNTTC 1 cut(s) 56
PfeI GAWTC 1 cut(s) 14
PleI GAGTC 1 cut(s) 21
PluTI GGCGCC 1 cut(s) 69
PpsI GAGTC 1 cut(s) 21
PscI ACATGT 1 cut(s) 80
PspN4I GGNNCC 2 cut(s) 67, 260
PspPI GGNCC 1 cut(s) 289
SapI GCTCTTC 2 cut(s) 190, 353
SaqAI TTAA 1 cut(s) 501
Sau3AI GATC 3 cut(s) 237, 365, 412
Sau96I GGNCC 1 cut(s) 289
SchI GAGTC 1 cut(s) 21
ScrFI CCNGG 2 cut(s) 63, 246
SduI GDGCHC 2 cut(s) 261, 520
SetI ASST 7 cut(s) 99, 108, 285, 407, 420, 465, 498
SfoI GGCGCC 1 cut(s) 67
SsiI CCGC 3 cut(s) 141, 204, 374
SspDI GGCGCC 1 cut(s) 65
StyD4I CCNGG 2 cut(s) 61, 244
TaqI TCGA 4 cut(s) 77, 253, 296, 458
TfiI GAWTC 1 cut(s) 14
Tru1I TTAA 1 cut(s) 501
Tru9I TTAA 1 cut(s) 501
TscAI CASTG 2 cut(s) 94, 322
TseFI GTSAC 1 cut(s) 379
Tsp45I GTSAC 1 cut(s) 379
TspDTI ATGAA 2 cut(s) 295, 368
TspRI CASTG 2 cut(s) 94, 322
XceI RCATGY 1 cut(s) 84
XmnI GAANNNNTTC 1 cut(s) 56
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.