Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000002

Orthogroup ID

OG0002661

Physical Location & Seq

Genomic Coordinates Reverse (-)

24656059 .. 24657786

Gene Structure 1728 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug02G0240300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1134 bp

ATGATGCAAGTCTACGGAAACCAGAGAGGTCCTTATGGTCCGATGAAGAATTCTATGGGTCCTGTCAAGAATCAAGATCCATGTAAGCCGCGCTATATGCCTTTTCCGGCTAATCACCGGAACCAGGTGCCGCCGGTCGGTCATTTTCAGCCCCAATTTCTTCCACCTCGACCAAATTTCTACCCATTCCCACAAACCCTCGTGCCACCTTATTCATCTGGTGGGTACAATTTTCAGGGGCAGGGTGATTCTACTCCAGCAAGTTCTGTCCCTCATCGTCTACCATCACAATGCGTAACTCCGGTTAACAGTGCTGGTCCAGTAGTCTCCGGTTTGCTTGGGTCTCTAATGGCACAAGGTGTGATCTCATTCAAAAATCAAAGCAGAGGAGGACTGGAGGGTACTAGTTCACTAAATTCTGTACCTCTTGTACCACAGCGAGGCGCATTTCCTGTTCAAAATGCTGCTGTTGATGTTCCAGTAGTAGTTCCTGGTTTGATTGATGCTCTCCTGGCACAAGGTGTGATTTCATTAAAGAAACAAAGCCCGGCACTGAAAGAGGAGAAGGATGCTGTGGTAGGATGTGAGTTTTCCGGCCTTGATCTAAATCTGCGTCGCGAATCTGTGGTAATCGGCTTGTATGAGGGTGGTCTGCCAAGGCAATGTAAAAGCTGTGGCCTCCGGTTCAAGTGTGAAGAACAGCACAGCAGCCATATGGATTGGCATGTCAAGAAGAACCGGATGTCCAAAACCCGCAAGCAGAAGCCCTCTCGTAACTTTTACGCAACTACAAGCATGTGGCTTGTTACTGAAGTAGAGGTTGAGGCTGAGGCATTACCTTCTGGTGTAGTTTTGTGCGCTGCTGACACCAGTGGCATGGCGGAAAAGAAAAGGAAGACTAGTGATGAAGAAATGGTTGTTCCTGCGGACGAGGATCAGAGTAAATGTGCCTTATGCACAGATGCTTTTGTTGAGTTTTATAGTCATGAGAAAGATGAGTGGATGTATAAAGGTGCTGTTTACTTGAATGCGCCTCAGGGGCCAGCGCCGGAGATGGATCGGTCACAGTTGGGTCCTATAGTCCATGCCAAATGCTGGTCGGAGGGTAGTAGGAAACGTCTGCGGAGGGGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006325 GO:0006464 GO:0006473 GO:0006475 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0016043 GO:0016569 GO:0016570 GO:0016573 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019538 GO:0031347 GO:0031348 GO:0036211 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043543 GO:0043966 GO:0043970 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0048519 GO:0048583 GO:0048585 GO:0050789 GO:0050896 GO:0051276 GO:0051704 GO:0051707 GO:0061647 GO:0065007 GO:0071704 GO:0071840 GO:0080134 GO:0098542 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

SIR2

Protein Analysis

377

Amino Acids

41.33

Weight (kDa)

8.91

Isoelectric Point (pI)

57.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf_PCFS4	PF23228	302 - 368	4.2e-28	PCFS4 zinc finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002661)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G09230 AT5G09230 AT5G09230 AT5G09230 AT5G09230 AT5G09230 AT5G09230
fragaria_vesca	FvH4_1g24990 FvH4_1g24990 FvH4_1g24990 FvH4_1g24990 FvH4_1g24990 FvH4_1g24990 FvH4_1g24990 FvH4_1g24990 FvH4_1g24990 FvH4_1g24990 FvH4_1g24990 FvH4_1g24990 FvH4_1g24990 FvH4_1g24990
malus_domestica	MD00G1091800.v1.1
prunus_persica	Prupe.4G275900_v2.0.a1 Prupe.4G275900_v2.0.a1
pyrus_communis	pycom01g00050
rosa_chinensis	RchiOBHm_Chr2g0122631
rosa_laevigata	RLG00000018696
rosa_multiflora	Rmu_sc0003133.1_g000010
rosa_roxburghii	Rroxscaffold_2G00121420 Rroxscaffold_4G00314940
rosa_rugosa	Rorug02G0240200 Rorug02G0240300 Rorug02G0240400 Rorug02G0240500 Rorug02G0240600
rosa_samantha	Rh2AG298700 Rh2BG307600 Rh2CG285700 Rh2DG322300
rosa_wichuraiana	Rw2G023750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	127
AccB7I	CCANNNNNTGG	1 cut(s)	1095
AccI	GTMKAC	2 cut(s)	12, 280
AccII	CGCG	2 cut(s)	91, 618
AciI	CCGC	6 cut(s)	89, 131, 754, 881, 926, 1123
AclWI	GGATC	3 cut(s)	71, 942, 1065
AcsI	RAATTY	3 cut(s)	49, 175, 415
AcuI	CTGAAG	1 cut(s)	831
AdeI	CACNNNGTG	2 cut(s)	359, 521
AfaI	GTAC	4 cut(s)	227, 403, 423, 432
AfiI	CCNNNNNNNGG	4 cut(s)	124, 137, 440, 1095
AgsI	TTSAA	4 cut(s)	373, 458, 688, 1027
AhlI	ACTAGT	2 cut(s)	404, 899
AjnI	CCWGG	3 cut(s)	123, 490, 510
AloI	GAACNNNNNNTCC	2 cut(s)	728, 760
AluBI	AGCT	1 cut(s)	672
AluI	AGCT	1 cut(s)	672
Alw26I	GTCTC	2 cut(s)	331, 348
AlwI	GGATC	3 cut(s)	71, 942, 1065
AoxI	GGCC	3 cut(s)	595, 676, 1040
ApeKI	GCWGC	3 cut(s)	464, 708, 860
ApoI	RAATTY	3 cut(s)	49, 175, 415
AspLEI	GCGC	5 cut(s)	93, 446, 860, 1033, 1048
AspS9I	GGNCC	6 cut(s)	29, 38, 59, 317, 1040, 1073
AsuC2I	CCSGG	1 cut(s)	548
AsuHPI	GGTGA	2 cut(s)	107, 257
AvaII	GGWCC	5 cut(s)	29, 38, 59, 317, 1073
AxyI	CCTNAGG	1 cut(s)	1035
BanI	GGYRCC	1 cut(s)	127
BauI	CACGAG	1 cut(s)	200
BbsI	GAAGAC	1 cut(s)	902
BbvCI	CCTCAGC	1 cut(s)	828
BbvI	GCAGC	3 cut(s)	451, 720, 847
BccI	CCATC	2 cut(s)	292, 1048
BciT130I	CCWGG	3 cut(s)	125, 492, 512
BcnI	CCSGG	1 cut(s)	548
BcoDI	GTCTC	2 cut(s)	331, 348
BcuI	ACTAGT	2 cut(s)	404, 899
BfaI	CTAG	2 cut(s)	405, 900
BfmI	CTRYAG	1 cut(s)	1077
BfoI	RGCGCY	1 cut(s)	1049
BglI	GCCNNNNNGGC	1 cut(s)	1039
BisI	GCNGC	5 cut(s)	89, 131, 465, 709, 861
BlsI	GCNGC	5 cut(s)	90, 132, 466, 710, 862
Bme1390I	CCNGG	4 cut(s)	125, 492, 512, 548
Bme18I	GGWCC	5 cut(s)	29, 38, 59, 317, 1073
BmgT120I	GGNCC	6 cut(s)	29, 38, 59, 317, 1040, 1073
BmiI	GGNNCC	5 cut(s)	60, 122, 129, 1041, 1074
BmrFI	CCNGG	4 cut(s)	125, 492, 512, 548
BmsI	GCATC	3 cut(s)	493, 559, 952
BpiI	GAAGAC	1 cut(s)	902
BpmI	CTGGAG	2 cut(s)	240, 416
Bpu10I	CCTNAGC	1 cut(s)	828
BpuMI	CCSGG	1 cut(s)	548
BsaBI	GATNNNNATC	1 cut(s)	606
BsaI	GGTCTC	1 cut(s)	348
BsaJI	CCNNGG	1 cut(s)	656
BsaWI	WCCGGW	5 cut(s)	117, 301, 329, 681, 738
Bsc4I	CCNNNNNNNGG	4 cut(s)	124, 137, 440, 1095
Bse118I	RCCGGY	1 cut(s)	133
Bse1I	ACTGG	4 cut(s)	320, 399, 479, 870
Bse21I	CCTNAGG	1 cut(s)	1035
Bse3DI	GCAATG	1 cut(s)	668
Bse8I	GATNNNNATC	1 cut(s)	606
BseBI	CCWGG	3 cut(s)	125, 492, 512
BseDI	CCNNGG	1 cut(s)	656
BseGI	GGATG	4 cut(s)	574, 587, 747, 1008
BseJI	GATNNNNATC	1 cut(s)	606
BseLI	CCNNNNNNNGG	4 cut(s)	124, 137, 440, 1095
BseMI	GCAATG	1 cut(s)	668
BseMII	CTCAG	2 cut(s)	819, 1049
BseNI	ACTGG	4 cut(s)	320, 399, 479, 870
BseRI	GAGGAG	2 cut(s)	402, 575
BseXI	GCAGC	3 cut(s)	451, 720, 847
Bsh1236I	CGCG	2 cut(s)	91, 618
Bsh1285I	CGRYCG	1 cut(s)	138
BshFI	GGCC	3 cut(s)	597, 678, 1042
BshNI	GGYRCC	1 cut(s)	127
BsiEI	CGRYCG	1 cut(s)	138
BslFI	GGGAC	1 cut(s)	254
BslI	CCNNNNNNNGG	4 cut(s)	124, 137, 440, 1095
BsmAI	GTCTC	2 cut(s)	331, 348
BsmFI	GGGAC	1 cut(s)	254
BsmI	GAATGC	1 cut(s)	1033
BsnI	GGCC	3 cut(s)	597, 678, 1042
Bso31I	GGTCTC	1 cut(s)	348
Bsp143I	GATC	5 cut(s)	76, 363, 601, 934, 1057
Bsp68I	TCGCGA	1 cut(s)	618
BspACI	CCGC	6 cut(s)	89, 131, 754, 881, 926, 1123
BspANI	GGCC	3 cut(s)	597, 678, 1042
BspCNI	CTCAG	2 cut(s)	820, 1048
BspFNI	CGCG	2 cut(s)	91, 618
BspHI	TCATGA	1 cut(s)	985
BspLI	GGNNCC	5 cut(s)	60, 122, 129, 1041, 1074
BspPI	GGATC	3 cut(s)	71, 942, 1065
BspT107I	GGYRCC	1 cut(s)	127
BspTNI	GGTCTC	1 cut(s)	348
BsrDI	GCAATG	1 cut(s)	668
BsrFI	RCCGGY	1 cut(s)	133
BsrI	ACTGG	4 cut(s)	320, 399, 479, 870
BssAI	RCCGGY	1 cut(s)	133
BssECI	CCNNGG	1 cut(s)	656
BssMI	GATC	5 cut(s)	76, 363, 601, 934, 1057
BssSI	CACGAG	1 cut(s)	200
BssT1I	CCWWGG	1 cut(s)	656
Bst2BI	CACGAG	1 cut(s)	200
Bst2UI	CCWGG	3 cut(s)	125, 492, 512
Bst4CI	ACNGT	2 cut(s)	311, 1068
BstC8I	GCNNGC	2 cut(s)	758, 1044
BstDEI	CTNAG	2 cut(s)	828, 1035
BstF5I	GGATG	4 cut(s)	574, 587, 747, 1008
BstFNI	CGCG	2 cut(s)	91, 618
BstH2I	RGCGCY	1 cut(s)	1049
BstHHI	GCGC	5 cut(s)	93, 446, 860, 1033, 1048
BstKTI	GATC	5 cut(s)	79, 366, 604, 937, 1060
BstMAI	GTCTC	2 cut(s)	331, 348
BstMBI	GATC	5 cut(s)	76, 363, 601, 934, 1057
BstMCI	CGRYCG	1 cut(s)	138
BstMWI	GCNNNNNNNGC	3 cut(s)	97, 512, 1039
BstNI	CCWGG	3 cut(s)	125, 492, 512
BstNSI	RCATGY	2 cut(s)	728, 799
BstSCI	CCNGG	4 cut(s)	123, 490, 510, 546
BstSFI	CTRYAG	1 cut(s)	1077
BstUI	CGCG	2 cut(s)	91, 618
BstV1I	GCAGC	3 cut(s)	451, 720, 847
BstV2I	GAAGAC	1 cut(s)	902
BstX2I	RGATCY	1 cut(s)	76
BstXI	CCANNNNNNTGG	1 cut(s)	877
BstYI	RGATCY	1 cut(s)	76
Bsu36I	CCTNAGG	1 cut(s)	1035
BsuRI	GGCC	3 cut(s)	597, 678, 1042
BtsCI	GGATG	4 cut(s)	574, 587, 747, 1008
BtsIMutI	CAGTG	3 cut(s)	316, 551, 877
BtuMI	TCGCGA	1 cut(s)	618
Cac8I	GCNNGC	2 cut(s)	758, 1044
CciI	TCATGA	1 cut(s)	985
CfoI	GCGC	5 cut(s)	93, 446, 860, 1033, 1048
Cfr10I	RCCGGY	1 cut(s)	133
Cfr13I	GGNCC	6 cut(s)	29, 38, 59, 317, 1040, 1073
CseI	GACGC	1 cut(s)	602
CsiI	ACCWGGT	1 cut(s)	123
Csp6I	GTAC	4 cut(s)	226, 402, 422, 431
CviAII	CATG	6 cut(s)	81, 725, 796, 877, 986, 1085
CviQI	GTAC	4 cut(s)	226, 402, 422, 431
DdeI	CTNAG	2 cut(s)	828, 1035
DpnI	GATC	5 cut(s)	78, 365, 603, 936, 1059
DpnII	GATC	5 cut(s)	76, 363, 601, 934, 1057
DraIII	CACNNNGTG	2 cut(s)	359, 521
EciI	GGCGGA	1 cut(s)	896
Eco130I	CCWWGG	1 cut(s)	656
Eco31I	GGTCTC	1 cut(s)	348
Eco47I	GGWCC	5 cut(s)	29, 38, 59, 317, 1073
Eco57I	CTGAAG	1 cut(s)	831
Eco81I	CCTNAGG	1 cut(s)	1035
EcoO109I	RGGNCCY	3 cut(s)	29, 59, 1073
EcoRI	GAATTC	1 cut(s)	49
EcoRII	CCWGG	3 cut(s)	123, 490, 510
EcoT14I	CCWWGG	1 cut(s)	656
ErhI	CCWWGG	1 cut(s)	656
FaeI	CATG	6 cut(s)	84, 728, 799, 880, 989, 1088
FaqI	GGGAC	1 cut(s)	254
FatI	CATG	6 cut(s)	80, 724, 795, 876, 985, 1084
FauI	CCCGC	1 cut(s)	761
FauNDI	CATATG	1 cut(s)	714
FblI	GTMKAC	2 cut(s)	12, 280
Fnu4HI	GCNGC	5 cut(s)	89, 131, 465, 709, 861
FokI	GGATG	4 cut(s)	581, 594, 754, 1015
Fsp4HI	GCNGC	5 cut(s)	89, 131, 465, 709, 861
FspBI	CTAG	2 cut(s)	405, 900
GlaI	GCGC	5 cut(s)	92, 445, 859, 1032, 1047
GluI	GCNGC	5 cut(s)	89, 131, 465, 709, 861
GsuI	CTGGAG	2 cut(s)	240, 416
HaeII	RGCGCY	1 cut(s)	1049
HaeIII	GGCC	3 cut(s)	597, 678, 1042
HgaI	GACGC	1 cut(s)	602
HhaI	GCGC	5 cut(s)	93, 446, 860, 1033, 1048
Hin1II	CATG	6 cut(s)	84, 728, 799, 880, 989, 1088
Hin6I	GCGC	5 cut(s)	91, 444, 858, 1031, 1046
HinP1I	GCGC	5 cut(s)	91, 444, 858, 1031, 1046
HincII	GTYRAC	1 cut(s)	307
HindII	GTYRAC	1 cut(s)	307
HinfI	GANTC	3 cut(s)	70, 248, 620
HpaI	GTTAAC	1 cut(s)	307
HphI	GGTGA	2 cut(s)	107, 257
Hpy166II	GTNNAC	5 cut(s)	13, 281, 307, 410, 1021
Hpy188I	TCNGA	3 cut(s)	42, 939, 1102
Hpy188III	TCNNGA	5 cut(s)	67, 74, 617, 730, 986
Hpy8I	GTNNAC	5 cut(s)	13, 281, 307, 410, 1021
Hpy99I	CGWCG	1 cut(s)	618
HpyAV	CCTTC	2 cut(s)	559, 849
HpyCH4III	ACNGT	2 cut(s)	311, 1068
HpyCH4IV	ACGT	1 cut(s)	1117
HpyCH4V	TGCA	2 cut(s)	7, 957
HpyF10VI	GCNNNNNNNGC	3 cut(s)	97, 512, 1039
HpyF3I	CTNAG	2 cut(s)	828, 1035
HpySE526I	ACGT	1 cut(s)	1117
Hsp92II	CATG	6 cut(s)	84, 728, 799, 880, 989, 1088
HspAI	GCGC	5 cut(s)	91, 444, 858, 1031, 1046
KspAI	GTTAAC	1 cut(s)	307
Kzo9I	GATC	5 cut(s)	76, 363, 601, 934, 1057
Lsp1109I	GCAGC	3 cut(s)	451, 720, 847
LweI	GCATC	3 cut(s)	493, 559, 952
MabI	ACCWGGT	1 cut(s)	123
MaeI	CTAG	2 cut(s)	405, 900
MaeII	ACGT	1 cut(s)	1117
MaeIII	GTNAC	4 cut(s)	295, 773, 805, 1062
MalI	GATC	5 cut(s)	78, 365, 603, 936, 1059
MboI	GATC	5 cut(s)	76, 363, 601, 934, 1057
MboII	GAAGA	6 cut(s)	58, 152, 707, 745, 907, 920
MflI	RGATCY	1 cut(s)	76
MluCI	AATT	5 cut(s)	49, 155, 175, 229, 415
MmeI	TCCRAC	1 cut(s)	1080
MseI	TTAA	2 cut(s)	306, 533
MslI	CAYNNNNRTG	1 cut(s)	289
MspR9I	CCNGG	4 cut(s)	125, 492, 512, 548
Mva1269I	GAATGC	1 cut(s)	1033
MvaI	CCWGG	3 cut(s)	125, 492, 512
MvnI	CGCG	2 cut(s)	91, 618
MwoI	GCNNNNNNNGC	3 cut(s)	97, 512, 1039
NciI	CCSGG	1 cut(s)	548
NdeI	CATATG	1 cut(s)	714
NdeII	GATC	5 cut(s)	76, 363, 601, 934, 1057
NlaIII	CATG	6 cut(s)	84, 728, 799, 880, 989, 1088
NlaIV	GGNNCC	5 cut(s)	60, 122, 129, 1041, 1074
NmuCI	GTSAC	1 cut(s)	1062
NruI	TCGCGA	1 cut(s)	618
NspI	RCATGY	2 cut(s)	728, 799
PagI	TCATGA	1 cut(s)	985
PctI	GAATGC	1 cut(s)	1033
PfeI	GAWTC	3 cut(s)	70, 248, 620
PflMI	CCANNNNNTGG	1 cut(s)	1095
PkrI	GCNGC	5 cut(s)	90, 132, 466, 710, 862
PpuMI	RGGWCCY	3 cut(s)	29, 59, 1073
Psp5II	RGGWCCY	3 cut(s)	29, 59, 1073
Psp6I	CCWGG	3 cut(s)	123, 490, 510
PspGI	CCWGG	3 cut(s)	123, 490, 510
PspN4I	GGNNCC	5 cut(s)	60, 122, 129, 1041, 1074
PspPI	GGNCC	6 cut(s)	29, 38, 59, 317, 1040, 1073
PspPPI	RGGWCCY	3 cut(s)	29, 59, 1073
PsuI	RGATCY	1 cut(s)	76
RruI	TCGCGA	1 cut(s)	618
RsaI	GTAC	4 cut(s)	227, 403, 423, 432
RsaNI	GTAC	4 cut(s)	226, 402, 422, 431
RseI	CAYNNNNRTG	1 cut(s)	289
SaqAI	TTAA	2 cut(s)	306, 533
SatI	GCNGC	5 cut(s)	89, 131, 465, 709, 861
Sau3AI	GATC	5 cut(s)	76, 363, 601, 934, 1057
Sau96I	GGNCC	6 cut(s)	29, 38, 59, 317, 1040, 1073
ScrFI	CCNGG	4 cut(s)	125, 492, 512, 548
SexAI	ACCWGGT	1 cut(s)	123
SfaNI	GCATC	3 cut(s)	493, 559, 952
SfcI	CTRYAG	1 cut(s)	1077
SinI	GGWCC	5 cut(s)	29, 38, 59, 317, 1073
SmiMI	CAYNNNNRTG	1 cut(s)	289
SpeI	ACTAGT	2 cut(s)	404, 899
Sse9I	AATT	5 cut(s)	49, 155, 175, 229, 415
SsiI	CCGC	6 cut(s)	89, 131, 754, 881, 926, 1123
SspMI	CTAG	2 cut(s)	405, 900
StyD4I	CCNGG	4 cut(s)	123, 490, 510, 546
StyI	CCWWGG	1 cut(s)	656
TaaI	ACNGT	2 cut(s)	311, 1068
TaiI	ACGT	1 cut(s)	1120
TaqI	TCGA	1 cut(s)	169
TaqII	GACCGA	2 cut(s)	128, 1050
TasI	AATT	5 cut(s)	49, 155, 175, 229, 415
TauI	GCSGC	2 cut(s)	91, 133
TfiI	GAWTC	3 cut(s)	70, 248, 620
Tru1I	TTAA	2 cut(s)	306, 533
Tru9I	TTAA	2 cut(s)	306, 533
TscAI	CASTG	3 cut(s)	316, 558, 877
TseFI	GTSAC	1 cut(s)	1062
TseI	GCWGC	3 cut(s)	464, 708, 860
Tsp45I	GTSAC	1 cut(s)	1062
TspDTI	ATGAA	4 cut(s)	59, 204, 519, 921
TspGWI	ACGGA	1 cut(s)	30
TspRI	CASTG	3 cut(s)	316, 558, 877
Van91I	CCANNNNNTGG	1 cut(s)	1095
VpaK11BI	GGWCC	5 cut(s)	29, 38, 59, 317, 1073
XapI	RAATTY	3 cut(s)	49, 175, 415
XceI	RCATGY	2 cut(s)	728, 799
XmiI	GTMKAC	2 cut(s)	12, 280
XspI	CTAG	2 cut(s)	405, 900

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.