Rorug02G0649000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0006659
Physical Location & Seq
Forward (+)
75816340 .. 75817888
1549 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0649000.1

Sequence Viewer

Length: 729 bp
ATGAACGGAGGAGGAGGAGGAGTGCAGATGCTGCCAGTTGGCTACCGGTTTGACCCTACGGATGAGGAGCTTGTGGTTCACTACTTGAGGAGAAAGGTCCATGGCCTCCCACTGCCGGTTTCCATCATTCCCGACTATAACAACAACTACTACTTTGATCATCCCTCCCACCTGCCAATGCCTCCAGGCGAGACTATGCAAAAGAGGTACTTCTTCTACAATAGTGAATATAATGATGAAAAAAGTAAGAACAAGAACAACCGCAGTAACTATAAGAGGGCTGCTGCTGCTGCTGGTTGTGGTTTTTGGAGGTCTATTGGGAAAGAGAAGCAAATTGTTGCTACTACTCATTCTGATCATCAAAACAGCTGCAGCCAAGCTCAATTAGCTGTCCTTGGGTTGAGGAAAACCTTGGTTTTCTGCGGCAGCCACAGGAAGAAGAAGACTAAGCACCATCACGGCCACTACCACTACCAACATCAATATGGAAGCCTCAGTAGTAGCCCTCGATGGTTCTTGCATGAATATAACCTTCTCGGTTCTGAGGTGTTAGGAAACAGCTGGATTCTCTGTAGTGTATTTCAGAAGAGGAGAAAGCCTGCGAGCAAGAAAAGAGGTCGGACCTCAACTTTGGATATTACAGGATCAGGCTATCTTCCCAAGCCTTCCAATTCATGTTCGAGTGAAATCACTACTGACGATCAGGAGGTCTTTTCCAAATGTTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

242

Amino Acids

27.59

Weight (kDa)

9.51

Isoelectric Point (pI)

56.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 12 - 177 1.7e-23 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 180
Acc36I ACCTGC 1 cut(s) 180
AciI CCGC 2 cut(s) 262, 423
AclWI GGATC 1 cut(s) 652
AcoI YGGCCR 1 cut(s) 460
AfaI GTAC 1 cut(s) 209
AfiI CCNNNNNNNGG 1 cut(s) 115
AgeI ACCGGT 1 cut(s) 45
AjnI CCWGG 1 cut(s) 184
AluBI AGCT 5 cut(s) 70, 369, 380, 389, 561
AluI AGCT 5 cut(s) 70, 369, 380, 389, 561
Alw26I GTCTC 1 cut(s) 185
AlwI GGATC 1 cut(s) 652
AlwNI CAGNNNCTG 1 cut(s) 31
AoxI GGCC 2 cut(s) 103, 460
ApeKI GCWGC 8 cut(s) 31, 281, 284, 287, 290, 369, 372, 426
ArsI GACNNNNNNTTYG 2 cut(s) 613, 645
AsiGI ACCGGT 1 cut(s) 45
AspS9I GGNCC 2 cut(s) 97, 621
AvaII GGWCC 2 cut(s) 97, 621
BbsI GAAGAC 1 cut(s) 449
BbvI GCAGC 8 cut(s) 18, 268, 271, 274, 277, 356, 384, 438
BccI CCATC 3 cut(s) 131, 462, 504
BceAI ACGGC 1 cut(s) 475
BciT130I CCWGG 1 cut(s) 186
BclI TGATCA 2 cut(s) 157, 355
BcoDI GTCTC 1 cut(s) 185
BfmI CTRYAG 2 cut(s) 370, 571
BfuAI ACCTGC 1 cut(s) 180
BisI GCNGC 9 cut(s) 32, 282, 285, 288, 291, 370, 373, 424, 427
BlsI GCNGC 9 cut(s) 33, 283, 286, 289, 292, 371, 374, 425, 428
Bme1390I CCNGG 1 cut(s) 186
Bme18I GGWCC 2 cut(s) 97, 621
BmgT120I GGNCC 2 cut(s) 97, 621
BmrFI CCNGG 1 cut(s) 186
BmsI GCATC 1 cut(s) 18
BpiI GAAGAC 1 cut(s) 449
BpmI CTGGAG 1 cut(s) 168
BpuEI CTTGAG 1 cut(s) 106
BsaJI CCNNGG 3 cut(s) 100, 394, 411
BsaWI WCCGGW 1 cut(s) 45
BsaXI ACNNNNNCTCC 2 cut(s) 6, 36
Bsc4I CCNNNNNNNGG 1 cut(s) 115
Bse118I RCCGGY 2 cut(s) 45, 115
Bse1I ACTGG 1 cut(s) 35
BseBI CCWGG 1 cut(s) 186
BseDI CCNNGG 3 cut(s) 100, 394, 411
BseGI GGATG 2 cut(s) 67, 160
BseLI CCNNNNNNNGG 1 cut(s) 115
BseMII CTCAG 2 cut(s) 508, 534
BseNI ACTGG 1 cut(s) 35
BseRI GAGGAG 7 cut(s) 24, 27, 30, 33, 80, 103, 604
BseXI GCAGC 8 cut(s) 18, 268, 271, 274, 277, 356, 384, 438
BsgI GTGCAG 1 cut(s) 44
BshFI GGCC 2 cut(s) 105, 462
BshTI ACCGGT 1 cut(s) 45
BsiSI CCGG 2 cut(s) 46, 116
BslI CCNNNNNNNGG 1 cut(s) 115
BsmAI GTCTC 1 cut(s) 185
BsnI GGCC 2 cut(s) 105, 462
Bsp143I GATC 4 cut(s) 157, 355, 644, 700
Bsp19I CCATGG 1 cut(s) 100
BspACI CCGC 2 cut(s) 262, 423
BspANI GGCC 2 cut(s) 105, 462
BspCNI CTCAG 2 cut(s) 507, 535
BspMAI CTGCAG 1 cut(s) 374
BspMI ACCTGC 1 cut(s) 180
BspPI GGATC 1 cut(s) 652
BsrFI RCCGGY 2 cut(s) 45, 115
BsrI ACTGG 1 cut(s) 35
BssAI RCCGGY 2 cut(s) 45, 115
BssECI CCNNGG 3 cut(s) 100, 394, 411
BssMI GATC 4 cut(s) 157, 355, 644, 700
BssT1I CCWWGG 3 cut(s) 100, 394, 411
Bst2UI CCWGG 1 cut(s) 186
Bst6I CTCTTC 1 cut(s) 581
BstAPI GCANNNNNTGC 1 cut(s) 31
BstC8I GCNNGC 2 cut(s) 600, 604
BstDEI CTNAG 3 cut(s) 447, 494, 543
BstDSI CCRYGG 1 cut(s) 100
BstF5I GGATG 2 cut(s) 67, 160
BstKTI GATC 4 cut(s) 160, 358, 647, 703
BstMAI GTCTC 1 cut(s) 185
BstMBI GATC 4 cut(s) 157, 355, 644, 700
BstMWI GCNNNNNNNGC 4 cut(s) 31, 287, 290, 386
BstNI CCWGG 1 cut(s) 186
BstSCI CCNGG 1 cut(s) 184
BstSFI CTRYAG 2 cut(s) 370, 571
BstV1I GCAGC 8 cut(s) 18, 268, 271, 274, 277, 356, 384, 438
BstV2I GAAGAC 1 cut(s) 449
BsuRI GGCC 2 cut(s) 105, 462
BtgI CCRYGG 1 cut(s) 100
BtsCI GGATG 2 cut(s) 67, 160
BtsI GCAGTG 1 cut(s) 110
BtsIMutI CAGTG 1 cut(s) 110
BveI ACCTGC 1 cut(s) 180
Cac8I GCNNGC 2 cut(s) 600, 604
CaiI CAGNNNCTG 1 cut(s) 31
Cfr10I RCCGGY 2 cut(s) 45, 115
Cfr13I GGNCC 2 cut(s) 97, 621
Csp6I GTAC 1 cut(s) 208
CspAI ACCGGT 1 cut(s) 45
CviAII CATG 3 cut(s) 101, 521, 675
CviQI GTAC 1 cut(s) 208
DdeI CTNAG 3 cut(s) 447, 494, 543
DpnI GATC 4 cut(s) 159, 357, 646, 702
DpnII GATC 4 cut(s) 157, 355, 644, 700
EaeI YGGCCR 1 cut(s) 460
Eam1104I CTCTTC 1 cut(s) 581
EarI CTCTTC 1 cut(s) 581
Eco130I CCWWGG 3 cut(s) 100, 394, 411
Eco47I GGWCC 2 cut(s) 97, 621
EcoRII CCWGG 1 cut(s) 184
EcoT14I CCWWGG 3 cut(s) 100, 394, 411
ErhI CCWWGG 3 cut(s) 100, 394, 411
FaeI CATG 3 cut(s) 104, 524, 678
FaiI YATR 9 cut(s) 102, 138, 197, 231, 273, 486, 522, 528, 676
FalI AAGNNNNNCTT 2 cut(s) 194, 226
FatI CATG 3 cut(s) 100, 520, 674
FbaI TGATCA 2 cut(s) 157, 355
Fnu4HI GCNGC 9 cut(s) 32, 282, 285, 288, 291, 370, 373, 424, 427
FokI GGATG 2 cut(s) 74, 147
Fsp4HI GCNGC 9 cut(s) 32, 282, 285, 288, 291, 370, 373, 424, 427
GluI GCNGC 9 cut(s) 32, 282, 285, 288, 291, 370, 373, 424, 427
GsuI CTGGAG 1 cut(s) 168
HaeIII GGCC 2 cut(s) 105, 462
HapII CCGG 2 cut(s) 46, 116
Hin1II CATG 3 cut(s) 104, 524, 678
HinfI GANTC 1 cut(s) 565
HpaII CCGG 2 cut(s) 46, 116
Hpy166II GTNNAC 1 cut(s) 79
Hpy188I TCNGA 4 cut(s) 355, 544, 585, 621
Hpy188III TCNNGA 2 cut(s) 131, 704
Hpy8I GTNNAC 1 cut(s) 79
HpyAV CCTTC 2 cut(s) 542, 675
HpyCH4V TGCA 4 cut(s) 25, 199, 372, 520
HpyF10VI GCNNNNNNNGC 4 cut(s) 31, 287, 290, 386
HpyF3I CTNAG 3 cut(s) 447, 494, 543
Hsp92II CATG 3 cut(s) 104, 524, 678
Ksp22I TGATCA 2 cut(s) 157, 355
Kzo9I GATC 4 cut(s) 157, 355, 644, 700
LmnI GCTCC 1 cut(s) 67
Lsp1109I GCAGC 8 cut(s) 18, 268, 271, 274, 277, 356, 384, 438
LweI GCATC 1 cut(s) 18
MaeIII GTNAC 1 cut(s) 266
MalI GATC 4 cut(s) 159, 357, 646, 702
MboI GATC 4 cut(s) 157, 355, 644, 700
MboII GAAGA 6 cut(s) 205, 448, 451, 454, 598, 647
MluCI AATT 3 cut(s) 333, 383, 670
MmeI TCCRAC 1 cut(s) 599
MslI CAYNNNNRTG 1 cut(s) 483
MspA1I CMGCKG 2 cut(s) 369, 561
MspI CCGG 2 cut(s) 46, 116
MspR9I CCNGG 1 cut(s) 186
MvaI CCWGG 1 cut(s) 186
MwoI GCNNNNNNNGC 4 cut(s) 31, 287, 290, 386
NcoI CCATGG 1 cut(s) 100
NdeII GATC 4 cut(s) 157, 355, 644, 700
NlaIII CATG 3 cut(s) 104, 524, 678
PaqCI CACCTGC 1 cut(s) 180
PfeI GAWTC 1 cut(s) 565
PinAI ACCGGT 1 cut(s) 45
PkrI GCNGC 9 cut(s) 33, 283, 286, 289, 292, 371, 374, 425, 428
Psp6I CCWGG 1 cut(s) 184
PspGI CCWGG 1 cut(s) 184
PspPI GGNCC 2 cut(s) 97, 621
PstI CTGCAG 1 cut(s) 374
PstNI CAGNNNCTG 1 cut(s) 31
PvuII CAGCTG 2 cut(s) 369, 561
RsaI GTAC 1 cut(s) 209
RsaNI GTAC 1 cut(s) 208
RseI CAYNNNNRTG 1 cut(s) 483
SatI GCNGC 9 cut(s) 32, 282, 285, 288, 291, 370, 373, 424, 427
Sau3AI GATC 4 cut(s) 157, 355, 644, 700
Sau96I GGNCC 2 cut(s) 97, 621
ScrFI CCNGG 1 cut(s) 186
SfaNI GCATC 1 cut(s) 18
SfcI CTRYAG 2 cut(s) 370, 571
SinI GGWCC 2 cut(s) 97, 621
SmiMI CAYNNNNRTG 1 cut(s) 483
SmlI CTYRAG 1 cut(s) 85
SmoI CTYRAG 1 cut(s) 85
Sse9I AATT 3 cut(s) 333, 383, 670
SsiI CCGC 2 cut(s) 262, 423
StyD4I CCNGG 1 cut(s) 184
StyI CCWWGG 3 cut(s) 100, 394, 411
TaqI TCGA 2 cut(s) 508, 680
TasI AATT 3 cut(s) 333, 383, 670
TauI GCSGC 1 cut(s) 426
TfiI GAWTC 1 cut(s) 565
TscAI CASTG 1 cut(s) 117
TseI GCWGC 8 cut(s) 31, 281, 284, 287, 290, 369, 372, 426
TspDTI ATGAA 4 cut(s) 17, 252, 537, 663
TspGWI ACGGA 2 cut(s) 21, 74
TspRI CASTG 1 cut(s) 117
VpaK11BI GGWCC 2 cut(s) 97, 621
XcmI CCANNNNNNNNNTGG 1 cut(s) 482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.