Rorug03G0147000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0003335
Physical Location & Seq
Reverse (-)
12054516 .. 12055300
785 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0147000.1

Sequence Viewer

Length: 528 bp
ATGAAGACAATGAGAAATCAAGAGGTTACGGGTTTAGACATGGCGAAATGTCTGATGCTCCTCTCTTGTAGGTTAGAGACCAAGTTCAAAAATTGTTCATCGTCGCCAAACGATGTGTTCGAGTGCAAAACCTGCAACCGCCAATTCTCATCGTTCCAAGCCCTTGGTGGCCACAGAGCGAGTCACAAGAAGCCGAGAACGTTACCGGGCAGTGATCAGGACTCGAAAAGAGAGACCAAATTGGCGCAAAGCAAGCCGAAAAAGCACGAGTGCTCCATATGCGGGATGGAGTTCAGTTTGGGGCAAGCTTTGGGGGGTCATATGCGAAGGCACAGAATGATGAACGCCGGGTTTTCTTCTTCGGTTCCTTCTGCGGCTGTTGTTGCTTCGAAGATTCCTGTGCTGATGAGGTCGAATAGTAAGAGGGTGATGTGTTTGGACATGGACTTGAACTTGACGCCTTTGGAAAATGACCTGAAGGTCTTGTTTGGGAACATGGCTCCAAAAGTTGATACTCTGGTTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

175

Amino Acids

19.39

Weight (kDa)

9.6

Isoelectric Point (pI)

44.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C2H2_6 PF13912 39 - 65 8.6e-13 C2H2-type zinc finger
zf-C2H2_6 PF13912 89 - 112 7.8e-09 C2H2-type zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 479
Acc36I ACCTGC 1 cut(s) 140
AciI CCGC 3 cut(s) 139, 282, 374
AclI AACGTT 1 cut(s) 200
AcoI YGGCCR 1 cut(s) 169
AcuI CTGAAG 1 cut(s) 497
AcyI GRCGYC 1 cut(s) 458
AfiI CCNNNNNNNGG 1 cut(s) 282
AgsI TTSAA 2 cut(s) 88, 451
AluBI AGCT 1 cut(s) 308
AluI AGCT 1 cut(s) 308
Alw21I GWGCWC 1 cut(s) 275
Alw26I GTCTC 2 cut(s) 71, 227
AoxI GGCC 1 cut(s) 169
AspLEI GCGC 1 cut(s) 247
AsuC2I CCSGG 2 cut(s) 207, 349
AsuHPI GGTGA 1 cut(s) 439
AsuII TTCGAA 1 cut(s) 389
BaeI ACNNNNGTAYC 1 cut(s) 504
BalI TGGCCA 1 cut(s) 171
BauI CACGAG 1 cut(s) 266
BbsI GAAGAC 1 cut(s) 11
Bbv12I GWGCWC 1 cut(s) 275
BccI CCATC 1 cut(s) 280
BclI TGATCA 1 cut(s) 214
BcnI CCSGG 2 cut(s) 207, 349
BcoDI GTCTC 2 cut(s) 71, 227
BfuAI ACCTGC 1 cut(s) 140
BisI GCNGC 1 cut(s) 375
BlsI GCNGC 1 cut(s) 376
Bme1390I CCNGG 2 cut(s) 207, 349
BmiI GGNNCC 2 cut(s) 366, 501
BmrFI CCNGG 2 cut(s) 207, 349
BmsI GCATC 1 cut(s) 45
BpiI GAAGAC 1 cut(s) 11
Bpu14I TTCGAA 1 cut(s) 389
BpuMI CCSGG 2 cut(s) 207, 349
BsaHI GRCGYC 1 cut(s) 458
BsaI GGTCTC 2 cut(s) 71, 227
BsaJI CCNNGG 1 cut(s) 163
BsaXI ACNNNNNCTCC 2 cut(s) 257, 287
Bsc4I CCNNNNNNNGG 1 cut(s) 282
BseDI CCNNGG 1 cut(s) 163
BseGI GGATG 1 cut(s) 291
BseLI CCNNNNNNNGG 1 cut(s) 282
BseRI GAGGAG 1 cut(s) 50
BshFI GGCC 1 cut(s) 171
BsiHKAI GWGCWC 1 cut(s) 275
BsiSI CCGG 2 cut(s) 206, 348
BslI CCNNNNNNNGG 1 cut(s) 282
BsmAI GTCTC 2 cut(s) 71, 227
BsnI GGCC 1 cut(s) 171
Bso31I GGTCTC 2 cut(s) 71, 227
Bsp119I TTCGAA 1 cut(s) 389
Bsp1286I GDGCHC 1 cut(s) 275
Bsp143I GATC 1 cut(s) 214
BspACI CCGC 3 cut(s) 139, 282, 374
BspANI GGCC 1 cut(s) 171
BspLI GGNNCC 2 cut(s) 366, 501
BspMI ACCTGC 1 cut(s) 140
BspT104I TTCGAA 1 cut(s) 389
BspTNI GGTCTC 2 cut(s) 71, 227
BssECI CCNNGG 1 cut(s) 163
BssMI GATC 1 cut(s) 214
BssNI GRCGYC 1 cut(s) 458
BssSI CACGAG 1 cut(s) 266
BssT1I CCWWGG 1 cut(s) 163
Bst2BI CACGAG 1 cut(s) 266
BstACI GRCGYC 1 cut(s) 458
BstAPI GCANNNNNTGC 1 cut(s) 132
BstBI TTCGAA 1 cut(s) 389
BstC8I GCNNGC 2 cut(s) 254, 306
BstF5I GGATG 1 cut(s) 291
BstHHI GCGC 1 cut(s) 247
BstKTI GATC 1 cut(s) 217
BstMAI GTCTC 2 cut(s) 71, 227
BstMBI GATC 1 cut(s) 214
BstMWI GCNNNNNNNGC 5 cut(s) 132, 253, 262, 279, 383
BstSCI CCNGG 2 cut(s) 205, 347
BstV2I GAAGAC 1 cut(s) 11
BstXI CCANNNNNNTGG 1 cut(s) 164
BsuRI GGCC 1 cut(s) 171
BtsCI GGATG 1 cut(s) 291
BtsI GCAGTG 1 cut(s) 217
BtsIMutI CAGTG 1 cut(s) 217
BveI ACCTGC 1 cut(s) 140
Cac8I GCNNGC 2 cut(s) 254, 306
CfoI GCGC 1 cut(s) 247
CseI GACGC 1 cut(s) 466
CviAII CATG 3 cut(s) 40, 442, 496
CviJI RGCY 7 cut(s) 161, 171, 193, 256, 308, 377, 500
CviKI_1 RGCY 7 cut(s) 161, 171, 193, 256, 308, 377, 500
DpnI GATC 1 cut(s) 216
DpnII GATC 1 cut(s) 214
DrdI GACNNNNNNGTC 1 cut(s) 479
DseDI GACNNNNNNGTC 1 cut(s) 479
EaeI YGGCCR 1 cut(s) 169
Eco130I CCWWGG 1 cut(s) 163
Eco31I GGTCTC 2 cut(s) 71, 227
Eco57I CTGAAG 1 cut(s) 497
EcoT14I CCWWGG 1 cut(s) 163
ErhI CCWWGG 1 cut(s) 163
FaeI CATG 3 cut(s) 43, 445, 499
FaiI YATR 7 cut(s) 41, 278, 280, 321, 323, 443, 497
FatI CATG 3 cut(s) 39, 441, 495
FauI CCCGC 1 cut(s) 275
FauNDI CATATG 2 cut(s) 278, 321
FbaI TGATCA 1 cut(s) 214
Fnu4HI GCNGC 1 cut(s) 375
FokI GGATG 1 cut(s) 298
Fsp4HI GCNGC 1 cut(s) 375
GlaI GCGC 1 cut(s) 246
GluI GCNGC 1 cut(s) 375
HaeIII GGCC 1 cut(s) 171
HapII CCGG 2 cut(s) 206, 348
HgaI GACGC 1 cut(s) 466
HhaI GCGC 1 cut(s) 247
Hin1I GRCGYC 1 cut(s) 458
Hin1II CATG 3 cut(s) 43, 445, 499
Hin6I GCGC 1 cut(s) 245
HinP1I GCGC 1 cut(s) 245
HindIII AAGCTT 1 cut(s) 306
HinfI GANTC 3 cut(s) 181, 221, 394
HpaII CCGG 2 cut(s) 206, 348
HphI GGTGA 1 cut(s) 439
Hpy188I TCNGA 1 cut(s) 54
Hpy188III TCNNGA 2 cut(s) 20, 218
Hpy99I CGWCG 1 cut(s) 106
HpyAV CCTTC 3 cut(s) 321, 378, 472
HpyCH4IV ACGT 1 cut(s) 200
HpyCH4V TGCA 2 cut(s) 126, 135
HpyF10VI GCNNNNNNNGC 5 cut(s) 132, 253, 262, 279, 383
HpySE526I ACGT 1 cut(s) 200
Hsp92I GRCGYC 1 cut(s) 458
Hsp92II CATG 3 cut(s) 43, 445, 499
HspAI GCGC 1 cut(s) 245
Ksp22I TGATCA 1 cut(s) 214
Kzo9I GATC 1 cut(s) 214
LmnI GCTCC 3 cut(s) 63, 278, 505
LpnPI CCDG 7 cut(s) 145, 203, 219, 361, 411, 488, 503
LweI GCATC 1 cut(s) 45
MaeII ACGT 1 cut(s) 200
MaeIII GTNAC 3 cut(s) 25, 182, 201
MalI GATC 1 cut(s) 216
MboI GATC 1 cut(s) 214
MboII GAAGA 4 cut(s) 16, 348, 351, 403
MhlI GDGCHC 1 cut(s) 275
MlsI TGGCCA 1 cut(s) 171
MluCI AATT 3 cut(s) 91, 143, 239
MluNI TGGCCA 1 cut(s) 171
MlyI GAGTC 2 cut(s) 190, 215
MnlI CCTC 4 cut(s) 16, 71, 402, 417
Mox20I TGGCCA 1 cut(s) 171
MscI TGGCCA 1 cut(s) 171
MseI TTAA 1 cut(s) 526
Msp20I TGGCCA 1 cut(s) 171
MspI CCGG 2 cut(s) 206, 348
MspR9I CCNGG 2 cut(s) 207, 349
MwoI GCNNNNNNNGC 5 cut(s) 132, 253, 262, 279, 383
NciI CCSGG 2 cut(s) 207, 349
NdeI CATATG 2 cut(s) 278, 321
NdeII GATC 1 cut(s) 214
NlaIII CATG 3 cut(s) 43, 445, 499
NlaIV GGNNCC 2 cut(s) 366, 501
NmeAIII GCCGAG 1 cut(s) 219
NmuCI GTSAC 1 cut(s) 182
NspV TTCGAA 1 cut(s) 389
PcsI WCGNNNNNNNCGW 1 cut(s) 117
PfeI GAWTC 1 cut(s) 394
PkrI GCNGC 1 cut(s) 376
PleI GAGTC 2 cut(s) 189, 215
PpsI GAGTC 2 cut(s) 189, 215
Psp1406I AACGTT 1 cut(s) 200
PspN4I GGNNCC 2 cut(s) 366, 501
SaqAI TTAA 1 cut(s) 526
SatI GCNGC 1 cut(s) 375
Sau3AI GATC 1 cut(s) 214
SchI GAGTC 2 cut(s) 190, 215
ScrFI CCNGG 2 cut(s) 207, 349
SduI GDGCHC 1 cut(s) 275
SetI ASST 8 cut(s) 27, 74, 134, 203, 310, 413, 477, 483
SfaNI GCATC 1 cut(s) 45
SfuI TTCGAA 1 cut(s) 389
Sse9I AATT 3 cut(s) 91, 143, 239
SsiI CCGC 3 cut(s) 139, 282, 374
StyD4I CCNGG 2 cut(s) 205, 347
StyI CCWWGG 1 cut(s) 163
TaiI ACGT 1 cut(s) 203
TaqI TCGA 4 cut(s) 120, 224, 389, 413
TasI AATT 3 cut(s) 91, 143, 239
TauI GCSGC 1 cut(s) 377
TfiI GAWTC 1 cut(s) 394
Tru1I TTAA 1 cut(s) 526
Tru9I TTAA 1 cut(s) 526
TscAI CASTG 1 cut(s) 217
TseFI GTSAC 1 cut(s) 182
Tsp45I GTSAC 1 cut(s) 182
TspDTI ATGAA 3 cut(s) 17, 87, 356
TspRI CASTG 1 cut(s) 217
XcmI CCANNNNNNNNNTGG 2 cut(s) 164, 283
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.