Rorug03G0176300

Nudix hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0016137
Physical Location & Seq
Reverse (-)
14951288 .. 14954625
3338 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0176300.1

Sequence Viewer

Length: 1077 bp
ATGGAAGACGTAAACCGAACCAAAGAGGCAAGCAACGAAGCAAGGATTGCCATACTGGAGCTGGCCAACATGATCAGCGTCCCTATGTCCCTAAACGCCGTCGTCCGCCTCAACGTCGCCGATGCCATCTGGCAATCCGGCTCTAACACCCCCCTCTCCGCCTCTCAGATCCTCGAACGTGTCCTCCCTGCCGGCGGTGGTGACGCCGAGAACCTCCAGCGCATCCTCCGCATGCTCACCAGCTACGGTGTGTTCTCCGAGCACTTCAACGCCGACGGCTCCGAGAGAAAGTTCTCCCTCACCGAGGTCGGCCAAACCTTAGTCACCAATCAAGATGGTCTCTCCTATGGGGCCTATGTCCTCCAGCACCACCAGGATGTACTAATGGGGGCGTGGCCACTGGTGCATGAGGCGGTGGTGGATCCCACGGTGGAGCCGTTCGTGAAGGCGAACGGTGAGCCGGCGTACCAGTACTACGGGAAGAGGCCGGAGATGAACGGGTTGATGCAAAAGGCAATGTCGGGAGTATCTGTGCCGTTCATCAAGGCAGTACTAGACGGTTACGATGGTTTTCAAGGGGTGACGAGACTGGTGGATGTGGGTGGCAGTGCGGGGGATTGCCTGAGAATGATCATAGACAAATATCCCAATGTACGGGAAGGCATTAACTTTGACCTACCTGAGGTCGTTGCGAAGGCCCCAGCTATCGCCGGAGTGACTCATGTTGGTGGTGACATGTTCAAGTCAATTCCTAGTGGTGATGTTATCTTCATGAAGTGGATTTTATCAACATGGACGGATAGCGAGTGCAAGCTGATACTGGAGAATTGTTTCAAGGCACTTCCTGCAGGAGGGAAATTGATTGCTTGTGAGCCGGTGGTGCCGAAGAAATCAGACGACAGCCACAGGACGCGTGCTCTTTTAGAGAATGACATATTCGTGATGACTATATATCGCACCAAGGGCAAGAATAGGACTGAAGAAGAATTCCAGCAGCTTGGTCACTCCGCTGGCTTCTCTCATTTCCGACCAATCTATATCGATTACTTTTATACCGTCTTAGAATTCCAAAAGTGA

Protein Analysis

358

Amino Acids

39.53

Weight (kDa)

5.45

Isoelectric Point (pI)

34.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dimerisation PF08100 19 - 109 4.5e-16 O-methyltransferase dimerisation domain
Methyltransf_2 PF00891 136 - 340 8e-47 O-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016137)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42070 AT2G42070
fragaria_vesca FvH4_6g22180
malus_domestica MD12G1056700.v1.1 MD14G1055000.v1.1
prunus_persica Prupe.7G077500_v2.0.a1 Prupe.7G077500_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0479531
rosa_laevigata RLG00000023561
rosa_multiflora Rmu_sc0004732.1_g000001
rosa_roxburghii Rroxscaffold_6G00402160
rosa_rugosa Rorug03G0176300
rosa_samantha Rh3AG227100 Rh3BG260300
rosa_wichuraiana Rw3G020290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 880
AccII CGCG 1 cut(s) 913
AciI CCGC 7 cut(s) 106, 159, 195, 229, 413, 611, 1008
AclWI GGATC 3 cut(s) 163, 416, 429
AcoI YGGCCR 3 cut(s) 63, 310, 395
AcsI RAATTY 2 cut(s) 986, 1064
AcuI CTGAAG 1 cut(s) 999
AcyI GRCGYC 1 cut(s) 204
AfaI GTAC 5 cut(s) 381, 467, 473, 552, 654
AfiI CCNNNNNNNGG 5 cut(s) 194, 304, 654, 682, 851
AflIII ACRYGT 3 cut(s) 178, 735, 911
AgsI TTSAA 4 cut(s) 268, 575, 742, 835
AjnI CCWGG 1 cut(s) 372
AloI GAACNNNNNNTCC 2 cut(s) 168, 200
AluBI AGCT 5 cut(s) 61, 243, 704, 814, 997
AluI AGCT 5 cut(s) 61, 243, 704, 814, 997
Alw21I GWGCWC 2 cut(s) 264, 919
Alw26I GTCTC 2 cut(s) 344, 580
AlwI GGATC 3 cut(s) 163, 416, 429
AoxI GGCC 6 cut(s) 63, 310, 351, 395, 485, 696
ApeKI GCWGC 1 cut(s) 994
ApoI RAATTY 2 cut(s) 986, 1064
Asp700I GAANNNNTTC 1 cut(s) 830
AspLEI GCGC 1 cut(s) 222
AspS9I GGNCC 2 cut(s) 351, 697
AsuHPI GGTGA 8 cut(s) 212, 229, 292, 316, 467, 592, 743, 770
AxyI CCTNAGG 1 cut(s) 681
BalI TGGCCA 2 cut(s) 65, 397
BamHI GGATCC 1 cut(s) 421
BanI GGYRCC 1 cut(s) 880
BbsI GAAGAC 1 cut(s) 12
Bbv12I GWGCWC 2 cut(s) 264, 919
BbvI GCAGC 1 cut(s) 1006
BccI CCATC 3 cut(s) 134, 329, 560
BceAI ACGGC 4 cut(s) 83, 292, 421, 520
BciT130I CCWGG 1 cut(s) 374
BclI TGATCA 2 cut(s) 72, 630
BcoDI GTCTC 2 cut(s) 344, 580
BfaI CTAG 2 cut(s) 554, 753
BfmI CTRYAG 1 cut(s) 846
BisI GCNGC 1 cut(s) 995
BlsI GCNGC 1 cut(s) 996
BmcAI AGTACT 2 cut(s) 473, 552
Bme1390I CCNGG 1 cut(s) 374
BmgT120I GGNCC 2 cut(s) 351, 697
BmiI GGNNCC 6 cut(s) 280, 352, 423, 435, 699, 882
BmrFI CCNGG 1 cut(s) 374
BmsI GCATC 3 cut(s) 112, 231, 495
BpiI GAAGAC 1 cut(s) 12
BpmI CTGGAG 4 cut(s) 77, 200, 347, 842
Bsa29I ATCGAT 1 cut(s) 1041
BsaHI GRCGYC 1 cut(s) 204
BsaI GGTCTC 1 cut(s) 344
BsaJI CCNNGG 3 cut(s) 303, 426, 960
BsaXI ACNNNNNCTCC 4 cut(s) 168, 198, 516, 546
Bsc4I CCNNNNNNNGG 5 cut(s) 194, 304, 654, 682, 851
Bse118I RCCGGY 3 cut(s) 191, 460, 874
Bse1I ACTGG 5 cut(s) 60, 405, 469, 594, 825
Bse21I CCTNAGG 1 cut(s) 681
Bse3DI GCAATG 1 cut(s) 522
BseBI CCWGG 1 cut(s) 374
BseCI ATCGAT 1 cut(s) 1041
BseDI CCNNGG 3 cut(s) 303, 426, 960
BseGI GGATG 3 cut(s) 222, 382, 601
BseLI CCNNNNNNNGG 5 cut(s) 194, 304, 654, 682, 851
BseMI GCAATG 1 cut(s) 522
BseMII CTCAG 3 cut(s) 179, 614, 672
BseNI ACTGG 5 cut(s) 60, 405, 469, 594, 825
BseXI GCAGC 1 cut(s) 1006
BseYI CCCAGC 1 cut(s) 700
Bsh1236I CGCG 1 cut(s) 913
BshFI GGCC 6 cut(s) 65, 312, 353, 397, 487, 698
BshNI GGYRCC 1 cut(s) 880
BshVI ATCGAT 1 cut(s) 1041
BsiHKAI GWGCWC 2 cut(s) 264, 919
BsiSI CCGG 6 cut(s) 138, 192, 461, 488, 711, 875
BslFI GGGAC 2 cut(s) 65, 73
BslI CCNNNNNNNGG 5 cut(s) 194, 304, 654, 682, 851
BsmAI GTCTC 2 cut(s) 344, 580
BsmFI GGGAC 2 cut(s) 65, 73
BsnI GGCC 6 cut(s) 65, 312, 353, 397, 487, 698
Bso31I GGTCTC 1 cut(s) 344
Bsp1286I GDGCHC 2 cut(s) 264, 919
Bsp143I GATC 4 cut(s) 72, 168, 421, 630
BspACI CCGC 7 cut(s) 106, 159, 195, 229, 413, 611, 1008
BspANI GGCC 6 cut(s) 65, 312, 353, 397, 487, 698
BspCNI CTCAG 3 cut(s) 178, 615, 673
BspDI ATCGAT 1 cut(s) 1041
BspFNI CGCG 1 cut(s) 913
BspHI TCATGA 1 cut(s) 771
BspLI GGNNCC 6 cut(s) 280, 352, 423, 435, 699, 882
BspMAI CTGCAG 1 cut(s) 850
BspPI GGATC 3 cut(s) 163, 416, 429
BspT107I GGYRCC 1 cut(s) 880
BspTNI GGTCTC 1 cut(s) 344
BsrDI GCAATG 1 cut(s) 522
BsrFI RCCGGY 3 cut(s) 191, 460, 874
BsrI ACTGG 5 cut(s) 60, 405, 469, 594, 825
BssAI RCCGGY 3 cut(s) 191, 460, 874
BssECI CCNNGG 3 cut(s) 303, 426, 960
BssMI GATC 4 cut(s) 72, 168, 421, 630
BssNI GRCGYC 1 cut(s) 204
BssT1I CCWWGG 1 cut(s) 960
Bst2UI CCWGG 1 cut(s) 374
Bst4CI ACNGT 5 cut(s) 248, 430, 455, 560, 1057
Bst6I CTCTTC 1 cut(s) 476
BstACI GRCGYC 1 cut(s) 204
BstAPI GCANNNNNTGC 2 cut(s) 47, 845
BstC8I GCNNGC 8 cut(s) 31, 63, 193, 233, 462, 812, 915, 1012
BstDEI CTNAG 5 cut(s) 165, 319, 623, 681, 1060
BstDSI CCRYGG 1 cut(s) 426
BstENI CCTNNNNNAGG 3 cut(s) 302, 680, 849
BstF5I GGATG 3 cut(s) 222, 382, 601
BstFNI CGCG 1 cut(s) 913
BstHHI GCGC 1 cut(s) 222
BstKTI GATC 4 cut(s) 75, 171, 424, 633
BstMAI GTCTC 2 cut(s) 344, 580
BstMBI GATC 4 cut(s) 72, 168, 421, 630
BstMWI GCNNNNNNNGC 6 cut(s) 47, 228, 403, 845, 880, 963
BstNI CCWGG 1 cut(s) 374
BstNSI RCATGY 2 cut(s) 235, 739
BstSCI CCNGG 1 cut(s) 372
BstSFI CTRYAG 1 cut(s) 846
BstUI CGCG 1 cut(s) 913
BstV1I GCAGC 1 cut(s) 1006
BstV2I GAAGAC 1 cut(s) 12
BstX2I RGATCY 2 cut(s) 168, 421
BstXI CCANNNNNNTGG 1 cut(s) 998
BstYI RGATCY 2 cut(s) 168, 421
Bsu15I ATCGAT 1 cut(s) 1041
Bsu36I CCTNAGG 1 cut(s) 681
BsuRI GGCC 6 cut(s) 65, 312, 353, 397, 487, 698
BsuTUI ATCGAT 1 cut(s) 1041
BtgI CCRYGG 1 cut(s) 426
BtsCI GGATG 3 cut(s) 222, 382, 601
BtsI GCAGTG 1 cut(s) 613
BtsIMutI CAGTG 2 cut(s) 398, 613
Cac8I GCNNGC 8 cut(s) 31, 63, 193, 233, 462, 812, 915, 1012
CciI TCATGA 1 cut(s) 771
CfoI GCGC 1 cut(s) 222
Cfr10I RCCGGY 3 cut(s) 191, 460, 874
Cfr13I GGNCC 2 cut(s) 351, 697
ClaI ATCGAT 1 cut(s) 1041
CseI GACGC 3 cut(s) 67, 212, 919
Csp6I GTAC 5 cut(s) 380, 466, 472, 551, 653
CviAII CATG 7 cut(s) 70, 232, 407, 722, 736, 772, 792
CviQI GTAC 5 cut(s) 380, 466, 472, 551, 653
DdeI CTNAG 5 cut(s) 165, 319, 623, 681, 1060
DpnI GATC 4 cut(s) 74, 170, 423, 632
DpnII GATC 4 cut(s) 72, 168, 421, 630
EaeI YGGCCR 3 cut(s) 63, 310, 395
Eam1104I CTCTTC 1 cut(s) 476
EarI CTCTTC 1 cut(s) 476
EciI GGCGGA 2 cut(s) 95, 148
Eco130I CCWWGG 1 cut(s) 960
Eco31I GGTCTC 1 cut(s) 344
Eco57I CTGAAG 1 cut(s) 999
Eco81I CCTNAGG 1 cut(s) 681
EcoNI CCTNNNNNAGG 3 cut(s) 302, 680, 849
EcoO109I RGGNCCY 2 cut(s) 351, 697
EcoRI GAATTC 2 cut(s) 986, 1064
EcoRII CCWGG 1 cut(s) 372
EcoT14I CCWWGG 1 cut(s) 960
ErhI CCWWGG 1 cut(s) 960
FaeI CATG 7 cut(s) 73, 235, 410, 725, 739, 775, 795
FaqI GGGAC 2 cut(s) 65, 73
FatI CATG 7 cut(s) 69, 231, 406, 721, 735, 771, 791
FauI CCCGC 1 cut(s) 604
FbaI TGATCA 2 cut(s) 72, 630
Fnu4HI GCNGC 1 cut(s) 995
FokI GGATG 3 cut(s) 209, 389, 608
Fsp4HI GCNGC 1 cut(s) 995
FspBI CTAG 2 cut(s) 554, 753
GlaI GCGC 1 cut(s) 221
GluI GCNGC 1 cut(s) 995
GsaI CCCAGC 1 cut(s) 704
GsuI CTGGAG 4 cut(s) 77, 200, 347, 842
HaeIII GGCC 6 cut(s) 65, 312, 353, 397, 487, 698
HapII CCGG 6 cut(s) 138, 192, 461, 488, 711, 875
HgaI GACGC 3 cut(s) 67, 212, 919
HhaI GCGC 1 cut(s) 222
Hin1I GRCGYC 1 cut(s) 204
Hin1II CATG 7 cut(s) 73, 235, 410, 725, 739, 775, 795
Hin6I GCGC 1 cut(s) 220
HinP1I GCGC 1 cut(s) 220
HinfI GANTC 1 cut(s) 718
HpaII CCGG 6 cut(s) 138, 192, 461, 488, 711, 875
HphI GGTGA 8 cut(s) 212, 229, 292, 316, 467, 592, 743, 770
Hpy166II GTNNAC 1 cut(s) 13
Hpy188I TCNGA 5 cut(s) 168, 259, 283, 895, 1028
Hpy188III TCNNGA 5 cut(s) 332, 442, 522, 772, 940
Hpy8I GTNNAC 1 cut(s) 13
Hpy99I CGWCG 3 cut(s) 104, 119, 278
HpyAV CCTTC 3 cut(s) 439, 653, 688
HpyCH4III ACNGT 5 cut(s) 248, 430, 455, 560, 1057
HpyCH4IV ACGT 3 cut(s) 9, 114, 178
HpyCH4V TGCA 4 cut(s) 406, 508, 810, 848
HpyF10VI GCNNNNNNNGC 6 cut(s) 47, 228, 403, 845, 880, 963
HpyF3I CTNAG 5 cut(s) 165, 319, 623, 681, 1060
HpySE526I ACGT 3 cut(s) 9, 114, 178
Hsp92I GRCGYC 1 cut(s) 204
Hsp92II CATG 7 cut(s) 73, 235, 410, 725, 739, 775, 795
HspAI GCGC 1 cut(s) 220
KroI GCCGGC 2 cut(s) 191, 460
KroNI GCCGGC 2 cut(s) 193, 462
Ksp22I TGATCA 2 cut(s) 72, 630
Kzo9I GATC 4 cut(s) 72, 168, 421, 630
LmnI GCTCC 3 cut(s) 58, 284, 433
Lsp1109I GCAGC 1 cut(s) 1006
LweI GCATC 3 cut(s) 112, 231, 495
MaeI CTAG 2 cut(s) 554, 753
MaeII ACGT 3 cut(s) 9, 114, 178
MaeIII GTNAC 7 cut(s) 200, 322, 560, 580, 715, 731, 1001
MalI GATC 4 cut(s) 74, 170, 423, 632
MboI GATC 4 cut(s) 72, 168, 421, 630
MboII GAAGA 6 cut(s) 17, 493, 760, 898, 992, 995
MflI RGATCY 2 cut(s) 168, 421
MhlI GDGCHC 2 cut(s) 264, 919
MlsI TGGCCA 2 cut(s) 65, 397
MluCI AATT 5 cut(s) 747, 826, 857, 986, 1064
MluI ACGCGT 1 cut(s) 911
MluNI TGGCCA 2 cut(s) 65, 397
MlyI GAGTC 1 cut(s) 712
MmeI TCCRAC 1 cut(s) 1051
Mox20I TGGCCA 2 cut(s) 65, 397
MroNI GCCGGC 2 cut(s) 191, 460
MroXI GAANNNNTTC 1 cut(s) 830
MscI TGGCCA 2 cut(s) 65, 397
MseI TTAA 1 cut(s) 666
MslI CAYNNNNRTG 3 cut(s) 375, 726, 938
Msp20I TGGCCA 2 cut(s) 65, 397
MspA1I CMGCKG 1 cut(s) 1010
MspI CCGG 6 cut(s) 138, 192, 461, 488, 711, 875
MspR9I CCNGG 1 cut(s) 374
MvaI CCWGG 1 cut(s) 374
MvnI CGCG 1 cut(s) 913
MwoI GCNNNNNNNGC 6 cut(s) 47, 228, 403, 845, 880, 963
NaeI GCCGGC 2 cut(s) 193, 462
NdeII GATC 4 cut(s) 72, 168, 421, 630
NgoMIV GCCGGC 2 cut(s) 191, 460
NlaIII CATG 7 cut(s) 73, 235, 410, 725, 739, 775, 795
NlaIV GGNNCC 6 cut(s) 280, 352, 423, 435, 699, 882
NmeAIII GCCGAG 1 cut(s) 232
NmuCI GTSAC 6 cut(s) 200, 322, 580, 715, 731, 1001
NspI RCATGY 2 cut(s) 235, 739
PaeI GCATGC 1 cut(s) 235
PagI TCATGA 1 cut(s) 771
PciI ACATGT 1 cut(s) 735
PcsI WCGNNNNNNNCGW 1 cut(s) 434
PdiI GCCGGC 2 cut(s) 193, 462
PdmI GAANNNNTTC 1 cut(s) 830
PkrI GCNGC 1 cut(s) 996
PleI GAGTC 1 cut(s) 712
PpsI GAGTC 1 cut(s) 712
PscI ACATGT 1 cut(s) 735
Psp6I CCWGG 1 cut(s) 372
PspFI CCCAGC 1 cut(s) 700
PspGI CCWGG 1 cut(s) 372
PspN4I GGNNCC 6 cut(s) 280, 352, 423, 435, 699, 882
PspPI GGNCC 2 cut(s) 351, 697
PstI CTGCAG 1 cut(s) 850
PsuI RGATCY 2 cut(s) 168, 421
RsaI GTAC 5 cut(s) 381, 467, 473, 552, 654
RsaNI GTAC 5 cut(s) 380, 466, 472, 551, 653
RseI CAYNNNNRTG 3 cut(s) 375, 726, 938
SaqAI TTAA 1 cut(s) 666
SatI GCNGC 1 cut(s) 995
Sau3AI GATC 4 cut(s) 72, 168, 421, 630
Sau96I GGNCC 2 cut(s) 351, 697
SbfI CCTGCAGG 1 cut(s) 850
ScaI AGTACT 2 cut(s) 473, 552
SchI GAGTC 1 cut(s) 712
ScrFI CCNGG 1 cut(s) 374
SdaI CCTGCAGG 1 cut(s) 850
SduI GDGCHC 2 cut(s) 264, 919
SfaNI GCATC 3 cut(s) 112, 231, 495
SfcI CTRYAG 1 cut(s) 846
SmiMI CAYNNNNRTG 3 cut(s) 375, 726, 938
SphI GCATGC 1 cut(s) 235
Sse8387I CCTGCAGG 1 cut(s) 850
Sse9I AATT 5 cut(s) 747, 826, 857, 986, 1064
SsiI CCGC 7 cut(s) 106, 159, 195, 229, 413, 611, 1008
SspMI CTAG 2 cut(s) 554, 753
StyD4I CCNGG 1 cut(s) 372
StyI CCWWGG 1 cut(s) 960
TaaI ACNGT 5 cut(s) 248, 430, 455, 560, 1057
TaiI ACGT 3 cut(s) 12, 117, 181
TaqI TCGA 2 cut(s) 174, 1041
TasI AATT 5 cut(s) 747, 826, 857, 986, 1064
TatI WGTACW 3 cut(s) 379, 471, 550
Tru1I TTAA 1 cut(s) 666
Tru9I TTAA 1 cut(s) 666
TscAI CASTG 2 cut(s) 405, 613
TseFI GTSAC 6 cut(s) 200, 322, 580, 715, 731, 1001
TseI GCWGC 1 cut(s) 994
Tsp45I GTSAC 6 cut(s) 200, 322, 580, 715, 731, 1001
TspDTI ATGAA 4 cut(s) 509, 529, 760, 788
TspGWI ACGGA 1 cut(s) 812
TspRI CASTG 2 cut(s) 405, 613
XagI CCTNNNNNAGG 3 cut(s) 302, 680, 849
XapI RAATTY 2 cut(s) 986, 1064
XceI RCATGY 2 cut(s) 235, 739
XcmI CCANNNNNNNNNTGG 1 cut(s) 58
XmnI GAANNNNTTC 1 cut(s) 830
XspI CTAG 2 cut(s) 554, 753
ZrmI AGTACT 2 cut(s) 473, 552
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.