Rorug03G0265400

histone-lysine N-methyltransferase, H3 lysine-9 specific

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0010169
Physical Location & Seq
Forward (+)
25812869 .. 25814396
1528 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0265400.1

Sequence Viewer

Length: 1158 bp
ATGGAGAAAGTTGAGAAAGTTGAGAATACGAGAAACATCTTTGACGCTGCCACGATTCTTGCTGCTGGTACCTGGCTGTTATTCTTGCTCATAGAGAAGGCTGGACTGAAAACAAATGTCAAGAAGGACTCTCATGCTACCAAGCTTCGTGGTGATGGAGCCTTGTTACTTTTCATGCGGTCTCTGATGAACTCTGATGTGGCTCCCACATTTCCTGCTGGAGCTTTCTTTGTGATGTTGTTGTTTCAAGGGAACGCTGGACCACTAACTACAAAGGTGAAGAAACAGAGTGTTCAGAATGTTAACAAGAGCAAGGACTCTTATGCTACCAAGCGTCCCGCAGTAGCCTTATTCAATTTCATGAGGGAGCAGTTCCACAAAACCTTCAAACAGACATTTTTTGGAGCGAAATCTGGATTAGCAGTGAGGCTCAAATGCATCTTGGCATTCACTTTGCTGCTCTTCACTCTTCCCTCAGTATATTCTGATGATTCTGGGATGGCTCACTTGAGACTGGCTCTTACCAATCCAAATGCTTATTGCTCAATGGTGGCTTGTGTTGCACCCATACCACAGCATTTTACTCTTCACGGACTGTGGGAACAGAATTTCGGCCCACCTGTTGGATCAACCTCCTTGACCTCCTATCATATCAGAGATGTTTCGGCTCGATATCTGCTCGAGTATTGGCCTGACCTAACTAAAGATCGGGCAAACAATGGGATTTACAATTGCTTATCCTTTTGGGACCATGAGTGGAGATCACATGGTGCACAGATATGTGCAAGAATTGATTATAGTAGAAAACGATATTTCCTTGTTTCTCTGCATCTTGCAGCCACATGCATCCCCAATCTTGAAACTGTGTTCGACACTTATGGGATTGTGAGAGATGGACAACAGCTCTATGACCCCAGGGATATTAGGAATGCCATCTTCCGTGAGCTGAGACTCTATGTGGTGTTGATATGCAGAAATGTGAAGAACAACAAGCTGGGGTTGTGGGAGATACACTTTTGTGTAAACCTGCATGGACAGTTGGAGAGTTGCACCAAGTGGTACAAGCCTGGAGCCTGCTATGAAGGATGCTATGGAAAAGGTGGTCTGCAACGGATCATTCTCCCTTCTGCTCCTCGTCTGATAAGTGAAGAACTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00310 ko01100 ko04146 ko04211 map00260 map00310 map01100 map04146 map04211
Pfam Domains
Protein Families
AT_hook Pre-SET SAD_SRA SET

Protein Analysis

385

Amino Acids

43.59

Weight (kDa)

9.31

Isoelectric Point (pI)

35.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 172 - 347 6.5e-20 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1035
Acc65I GGTACC 1 cut(s) 68
AccB1I GGYRCC 1 cut(s) 68
AccB7I CCANNNNNTGG 1 cut(s) 623
AciI CCGC 2 cut(s) 178, 339
AclWI GGATC 2 cut(s) 634, 1121
AcsI RAATTY 1 cut(s) 607
AdeI CACNNNGTG 2 cut(s) 770, 1056
AfaI GTAC 2 cut(s) 70, 1061
AfiI CCNNNNNNNGG 1 cut(s) 623
AgsI TTSAA 4 cut(s) 248, 355, 388, 860
AjnI CCWGG 3 cut(s) 71, 914, 1066
AleI CACNNNNGTG 1 cut(s) 1017
AluBI AGCT 5 cut(s) 145, 224, 904, 946, 994
AluI AGCT 5 cut(s) 145, 224, 904, 946, 994
Alw21I GWGCWC 1 cut(s) 775
Alw26I GTCTC 3 cut(s) 186, 505, 943
Alw44I GTGCAC 1 cut(s) 771
AlwI GGATC 2 cut(s) 634, 1121
Ama87I CYCGRG 1 cut(s) 680
AoxI GGCC 2 cut(s) 613, 689
ApaLI GTGCAC 1 cut(s) 771
ApeKI GCWGC 4 cut(s) 47, 62, 457, 836
ApoI RAATTY 1 cut(s) 607
Asp718I GGTACC 1 cut(s) 68
AspS9I GGNCC 3 cut(s) 260, 614, 748
AsuHPI GGTGA 2 cut(s) 164, 289
AvaI CYCGRG 1 cut(s) 680
AvaII GGWCC 2 cut(s) 260, 748
BaeGI GKGCMC 1 cut(s) 775
BanI GGYRCC 1 cut(s) 68
Bbv12I GWGCWC 1 cut(s) 775
BbvI GCAGC 4 cut(s) 34, 49, 444, 848
BccI CCATC 4 cut(s) 149, 493, 887, 941
BciT130I CCWGG 3 cut(s) 73, 916, 1068
BcoDI GTCTC 3 cut(s) 186, 505, 943
BfuAI ACCTGC 1 cut(s) 1035
BisI GCNGC 4 cut(s) 48, 63, 458, 837
BlsI GCNGC 4 cut(s) 49, 64, 459, 838
Bme1390I CCNGG 3 cut(s) 73, 916, 1068
Bme18I GGWCC 2 cut(s) 260, 748
BmeT110I CYCGRG 1 cut(s) 680
BmgT120I GGNCC 3 cut(s) 260, 614, 748
BmiI GGNNCC 5 cut(s) 70, 160, 204, 749, 1072
BmrFI CCNGG 3 cut(s) 73, 916, 1068
BmsI GCATC 4 cut(s) 447, 838, 855, 1076
BplI GAGNNNNNCTC 2 cut(s) 502, 534
BpmI CTGGAG 2 cut(s) 240, 1089
BpuEI CTTGAG 1 cut(s) 529
BsaI GGTCTC 1 cut(s) 186
BsaJI CCNNGG 2 cut(s) 914, 915
Bsc4I CCNNNNNNNGG 1 cut(s) 623
Bse1I ACTGG 1 cut(s) 519
BseBI CCWGG 3 cut(s) 73, 916, 1068
BseDI CCNNGG 2 cut(s) 914, 915
BseGI GGATG 3 cut(s) 504, 846, 1091
BseLI CCNNNNNNNGG 1 cut(s) 623
BseMII CTCAG 2 cut(s) 489, 938
BseNI ACTGG 1 cut(s) 519
BseRI GAGGAG 1 cut(s) 1122
BseSI GKGCMC 1 cut(s) 775
BseXI GCAGC 4 cut(s) 34, 49, 444, 848
BseYI CCCAGC 1 cut(s) 994
BshFI GGCC 2 cut(s) 615, 691
BshNI GGYRCC 1 cut(s) 68
BsiHKAI GWGCWC 1 cut(s) 775
BsiHKCI CYCGRG 1 cut(s) 680
BslFI GGGAC 2 cut(s) 321, 761
BslI CCNNNNNNNGG 1 cut(s) 623
BsmAI GTCTC 3 cut(s) 186, 505, 943
BsmFI GGGAC 2 cut(s) 321, 761
BsmI GAATGC 2 cut(s) 446, 934
BsnI GGCC 2 cut(s) 615, 691
Bso31I GGTCTC 1 cut(s) 186
BsoBI CYCGRG 1 cut(s) 680
Bsp1286I GDGCHC 1 cut(s) 775
Bsp143I GATC 4 cut(s) 626, 706, 761, 1113
BspACI CCGC 2 cut(s) 178, 339
BspANI GGCC 2 cut(s) 615, 691
BspCNI CTCAG 2 cut(s) 488, 939
BspHI TCATGA 1 cut(s) 360
BspLI GGNNCC 5 cut(s) 70, 160, 204, 749, 1072
BspMI ACCTGC 1 cut(s) 1035
BspPI GGATC 2 cut(s) 634, 1121
BspQI GCTCTTC 1 cut(s) 467
BspT107I GGYRCC 1 cut(s) 68
BspTNI GGTCTC 1 cut(s) 186
BsrI ACTGG 1 cut(s) 519
BssECI CCNNGG 2 cut(s) 914, 915
BssMI GATC 4 cut(s) 626, 706, 761, 1113
Bst2UI CCWGG 3 cut(s) 73, 916, 1068
Bst4CI ACNGT 3 cut(s) 597, 865, 1038
Bst6I CTCTTC 3 cut(s) 467, 474, 591
BstC8I GCNNGC 1 cut(s) 1075
BstDEI CTNAG 2 cut(s) 475, 947
BstF5I GGATG 3 cut(s) 504, 846, 1091
BstKTI GATC 4 cut(s) 629, 709, 764, 1116
BstMAI GTCTC 3 cut(s) 186, 505, 943
BstMBI GATC 4 cut(s) 626, 706, 761, 1113
BstMWI GCNNNNNNNGC 1 cut(s) 560
BstNI CCWGG 3 cut(s) 73, 916, 1068
BstNSI RCATGY 1 cut(s) 846
BstSCI CCNGG 3 cut(s) 71, 914, 1066
BstSLI GKGCMC 1 cut(s) 775
BstV1I GCAGC 4 cut(s) 34, 49, 444, 848
BsuRI GGCC 2 cut(s) 615, 691
BtsCI GGATG 3 cut(s) 504, 846, 1091
BtsI GCAGTG 1 cut(s) 429
BtsIMutI CAGTG 1 cut(s) 429
BveI ACCTGC 1 cut(s) 1035
Cac8I GCNNGC 1 cut(s) 1075
CciI TCATGA 1 cut(s) 360
Cfr13I GGNCC 3 cut(s) 260, 614, 748
CseI GACGC 2 cut(s) 53, 323
Csp6I GTAC 2 cut(s) 69, 1060
CspCI CAANNNNNGTGG 4 cut(s) 40, 75, 130, 165
CviAII CATG 7 cut(s) 134, 175, 361, 752, 767, 843, 1031
CviQI GTAC 2 cut(s) 69, 1060
DdeI CTNAG 2 cut(s) 475, 947
DpnI GATC 4 cut(s) 628, 708, 763, 1115
DpnII GATC 4 cut(s) 626, 706, 761, 1113
DraIII CACNNNGTG 2 cut(s) 770, 1056
Eam1104I CTCTTC 3 cut(s) 467, 474, 591
EarI CTCTTC 3 cut(s) 467, 474, 591
Eco31I GGTCTC 1 cut(s) 186
Eco32I GATATC 1 cut(s) 674
Eco47I GGWCC 2 cut(s) 260, 748
Eco88I CYCGRG 1 cut(s) 680
EcoRII CCWGG 3 cut(s) 71, 914, 1066
EcoRV GATATC 1 cut(s) 674
EcoT22I ATGCAT 2 cut(s) 440, 848
FaeI CATG 7 cut(s) 137, 178, 364, 755, 770, 846, 1034
FaqI GGGAC 2 cut(s) 321, 761
FatI CATG 7 cut(s) 133, 174, 360, 751, 766, 842, 1030
FauI CCCGC 1 cut(s) 346
Fnu4HI GCNGC 4 cut(s) 48, 63, 458, 837
FokI GGATG 3 cut(s) 511, 833, 1098
Fsp4HI GCNGC 4 cut(s) 48, 63, 458, 837
GluI GCNGC 4 cut(s) 48, 63, 458, 837
GsaI CCCAGC 1 cut(s) 998
GsuI CTGGAG 2 cut(s) 240, 1089
HaeIII GGCC 2 cut(s) 615, 691
HgaI GACGC 2 cut(s) 53, 323
Hin1II CATG 7 cut(s) 137, 178, 364, 755, 770, 846, 1034
HincII GTYRAC 1 cut(s) 304
HindII GTYRAC 1 cut(s) 304
HindIII AAGCTT 1 cut(s) 143
HinfI GANTC 5 cut(s) 55, 128, 317, 491, 951
HpaI GTTAAC 1 cut(s) 304
HphI GGTGA 2 cut(s) 164, 289
Hpy166II GTNNAC 3 cut(s) 304, 773, 1024
Hpy188I TCNGA 6 cut(s) 186, 196, 297, 487, 656, 1140
Hpy188III TCNNGA 4 cut(s) 121, 361, 414, 857
Hpy8I GTNNAC 3 cut(s) 304, 773, 1024
HpyAV CCTTC 5 cut(s) 91, 118, 394, 1076, 1134
HpyCH4III ACNGT 3 cut(s) 597, 865, 1038
HpyF10VI GCNNNNNNNGC 1 cut(s) 560
HpyF3I CTNAG 2 cut(s) 475, 947
Hsp92II CATG 7 cut(s) 137, 178, 364, 755, 770, 846, 1034
KpnI GGTACC 1 cut(s) 72
KspAI GTTAAC 1 cut(s) 304
Kzo9I GATC 4 cut(s) 626, 706, 761, 1113
LguI GCTCTTC 1 cut(s) 467
LmnI GCTCC 7 cut(s) 158, 208, 221, 367, 404, 1070, 1135
Lsp1109I GCAGC 4 cut(s) 34, 49, 444, 848
LweI GCATC 4 cut(s) 447, 838, 855, 1076
MaeIII GTNAC 1 cut(s) 165
MalI GATC 4 cut(s) 628, 708, 763, 1115
MboI GATC 4 cut(s) 626, 706, 761, 1113
MboII GAAGA 6 cut(s) 292, 454, 461, 578, 928, 994
MfeI CAATTG 1 cut(s) 730
MhlI GDGCHC 1 cut(s) 775
MluCI AATT 4 cut(s) 355, 607, 730, 789
MlyI GAGTC 3 cut(s) 122, 311, 945
MmeI TCCRAC 2 cut(s) 604, 1020
MnlI CCTC 6 cut(s) 357, 420, 484, 643, 652, 1143
Mph1103I ATGCAT 2 cut(s) 440, 848
MseI TTAA 1 cut(s) 303
MslI CAYNNNNRTG 2 cut(s) 778, 1017
MspR9I CCNGG 3 cut(s) 73, 916, 1068
MunI CAATTG 1 cut(s) 730
Mva1269I GAATGC 2 cut(s) 446, 934
MvaI CCWGG 3 cut(s) 73, 916, 1068
MwoI GCNNNNNNNGC 1 cut(s) 560
NdeII GATC 4 cut(s) 626, 706, 761, 1113
NlaIII CATG 7 cut(s) 137, 178, 364, 755, 770, 846, 1034
NlaIV GGNNCC 5 cut(s) 70, 160, 204, 749, 1072
NsiI ATGCAT 2 cut(s) 440, 848
NspI RCATGY 1 cut(s) 846
OliI CACNNNNGTG 1 cut(s) 1017
PaeR7I CTCGAG 1 cut(s) 680
PagI TCATGA 1 cut(s) 360
PasI CCCWGGG 1 cut(s) 915
PciSI GCTCTTC 1 cut(s) 467
PctI GAATGC 2 cut(s) 446, 934
PfeI GAWTC 2 cut(s) 55, 491
PflMI CCANNNNNTGG 1 cut(s) 623
PkrI GCNGC 4 cut(s) 49, 64, 459, 838
PleI GAGTC 3 cut(s) 122, 311, 945
PpsI GAGTC 3 cut(s) 122, 311, 945
Psp6I CCWGG 3 cut(s) 71, 914, 1066
PspFI CCCAGC 1 cut(s) 994
PspGI CCWGG 3 cut(s) 71, 914, 1066
PspN4I GGNNCC 5 cut(s) 70, 160, 204, 749, 1072
PspPI GGNCC 3 cut(s) 260, 614, 748
PspXI VCTCGAGB 1 cut(s) 680
RsaI GTAC 2 cut(s) 70, 1061
RsaNI GTAC 2 cut(s) 69, 1060
RseI CAYNNNNRTG 2 cut(s) 778, 1017
SapI GCTCTTC 1 cut(s) 467
SaqAI TTAA 1 cut(s) 303
SatI GCNGC 4 cut(s) 48, 63, 458, 837
Sau3AI GATC 4 cut(s) 626, 706, 761, 1113
Sau96I GGNCC 3 cut(s) 260, 614, 748
SchI GAGTC 3 cut(s) 122, 311, 945
ScrFI CCNGG 3 cut(s) 73, 916, 1068
SduI GDGCHC 1 cut(s) 775
SfaNI GCATC 4 cut(s) 447, 838, 855, 1076
Sfr274I CTCGAG 1 cut(s) 680
SinI GGWCC 2 cut(s) 260, 748
SlaI CTCGAG 1 cut(s) 680
SmiMI CAYNNNNRTG 2 cut(s) 778, 1017
SmlI CTYRAG 2 cut(s) 508, 680
SmoI CTYRAG 2 cut(s) 508, 680
Sse9I AATT 4 cut(s) 355, 607, 730, 789
SsiI CCGC 2 cut(s) 178, 339
StyD4I CCNGG 3 cut(s) 71, 914, 1066
TaaI ACNGT 3 cut(s) 597, 865, 1038
TaqI TCGA 3 cut(s) 670, 681, 870
TasI AATT 4 cut(s) 355, 607, 730, 789
TfiI GAWTC 2 cut(s) 55, 491
Tru1I TTAA 1 cut(s) 303
Tru9I TTAA 1 cut(s) 303
TscAI CASTG 1 cut(s) 429
TseI GCWGC 4 cut(s) 47, 62, 457, 836
TspDTI ATGAA 4 cut(s) 163, 203, 349, 1095
TspGWI ACGGA 3 cut(s) 606, 929, 1126
TspRI CASTG 1 cut(s) 429
Van91I CCANNNNNTGG 1 cut(s) 623
VneI GTGCAC 1 cut(s) 771
VpaK11BI GGWCC 2 cut(s) 260, 748
XapI RAATTY 1 cut(s) 607
XceI RCATGY 1 cut(s) 846
XhoI CTCGAG 1 cut(s) 680
Zsp2I ATGCAT 2 cut(s) 440, 848
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.