Rorug04G0033200

Yip1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0012587
Physical Location & Seq
Reverse (-)
4959739 .. 4961787
2049 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0033200.1

Sequence Viewer

Length: 1470 bp
ATGTCTGTAAATCATAAGAGTGGTAAAGATCATTTAGTTATCGATATTATTGACAAGAAATGCATCTTCAGAGTTCCTAACGTGCTCCGGAGACAAAACCCCGAGGCATATACACCTGACGTTGTTTCAATCGGACCTTTACATCATTATCGAGAAAAGCGAGGCAAGGAAGAGGGTAAAGGAGGCAAGGAAGACAAAGAAGACAAAGGCGAGGAAGATTTCCAACTCCTGAAACGAGTGAAAAAAAGCTATTTGAATGAAATTCTCTCACGTATGAAGAATATAACTTTGGAAGAGTTGACCACAAAAGTTACTGAGCTCTCAGATAAGAAGAATGAAGGTGGGTTTGAGGAACGAGCTCGCAACTTTTATGCAGAACCACTTGATCATATTCCTTCCAAAGACTTCATCGAGATGATGATAGTTGATGGTTGCTTCCTAGTTCAACTATTTCGGAAGTGTAAACATTCCAATCTCAGGGCCTCAGATGACCTGGTGTTCAACATGGACTGTATGTTTCATTTCCTATGCCATGACATTTTGCTCTTAGAGAATCAACTACCTTGGTTTGTTATCCACAGTTTGTATAGCCTTACCCTTGAAATATACCCTGATGAAGCCTCTCTCTCTGTTCTCATCCTTAAAGCCTTCAGCATACTACCATCACTGAAGCAAAGTTGCTCATCTTATAATAAGCATCTCCGCAAAAGCAGGTGTCATTGTGATGCTGATTATCTTCACATACTTGATCTGGTAAGAGCTTCCATAGTTATTCCATTGAAGACCATAGATGCGAGAGTAGCTAGAAAAGAATCTATGTCCGACCGAGCTGAAGAACATCAAGTGCATAAAAGGACCGTAGACAAGGCCGAGAGTAAATCTGTTCATGAAGCTATCTTTGACCCAGATCTACATCAAATACGTACCGCAACCGCTCTCTCAAAGGCAGACATTAAATTCGAAAAAGTCATAAGGGAGAGCATAATGGACATTCGATTCAGAGTGGGTGGGTGGTTCAGGAATGGGATTCTAGAGATTCCACAGCTAAACGTTGGAATGTCATCGGAAACATTATTCAGGAACCTTATTGCAATTGAGCAATGCTTCCATGGTTATTCAAACGAGATAACATCTTATGCCATCTTTATGGATAACCTCATCTCTTCAAAGGAAGATATGGAATTACTTTGCAAGGAAAAAGTAATTGGTAACTGGATGAGTGATGAAGACGGTTGTGAGTTCTTCAGCAATCTTTACAAGGACATCCCGCCCAACAAGTTCTACTATGTTGGTCTGTGCGAAAAAGTGAATGCTCGTTACAGATCGAGATGGTATACATGGTTGGCTTTACTCAAGAGTGAAAAATTTTCTAACCCCTGGAGAATTTTAGCTTTCGGGATAGGTATTATCGTTTTGACTCTCACCGCCTGGAGTCAGACAAACAACATTAGGGTTAACATGCATAAGTGA

Protein Analysis

489

Amino Acids

56.83

Weight (kDa)

7.59

Isoelectric Point (pI)

46.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 22 - 474 1.5e-96 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 690
AarI CACCTGC 1 cut(s) 702
Acc36I ACCTGC 1 cut(s) 702
AccBSI CCGCTC 1 cut(s) 935
AccI GTMKAC 2 cut(s) 861, 1334
AccIII TCCGGA 1 cut(s) 87
AciI CCGC 5 cut(s) 703, 927, 933, 1268, 1425
AclI AACGTT 1 cut(s) 1050
AcsI RAATTY 4 cut(s) 261, 956, 1364, 1383
AcuI CTGAAG 5 cut(s) 52, 634, 689, 852, 1228
AfaI GTAC 1 cut(s) 925
AfiI CCNNNNNNNGG 1 cut(s) 477
AgsI TTSAA 8 cut(s) 129, 256, 446, 502, 602, 781, 1119, 1167
AjnI CCWGG 3 cut(s) 492, 1376, 1427
AjuI GAANNNNNNNTTGG 4 cut(s) 272, 304, 1188, 1220
AluBI AGCT 9 cut(s) 249, 319, 359, 761, 803, 830, 893, 1045, 1391
AluI AGCT 9 cut(s) 249, 319, 359, 761, 803, 830, 893, 1045, 1391
Alw21I GWGCWC 3 cut(s) 87, 321, 361
Alw26I GTCTC 1 cut(s) 85
Ama87I CYCGRG 1 cut(s) 101
Aor13HI TCCGGA 1 cut(s) 87
AoxI GGCC 2 cut(s) 480, 867
ApoI RAATTY 4 cut(s) 261, 956, 1364, 1383
ArsI GACNNNNNNTTYG 2 cut(s) 941, 973
AspS9I GGNCC 3 cut(s) 134, 480, 855
AsuHPI GGTGA 1 cut(s) 1414
AsuII TTCGAA 1 cut(s) 960
AvaI CYCGRG 1 cut(s) 101
AvaII GGWCC 2 cut(s) 134, 855
BanII GRGCYC 2 cut(s) 321, 361
BbsI GAAGAC 4 cut(s) 198, 207, 788, 1233
Bbv12I GWGCWC 3 cut(s) 87, 321, 361
BccI CCATC 4 cut(s) 422, 670, 1148, 1323
BciT130I CCWGG 3 cut(s) 494, 1378, 1429
BclI TGATCA 1 cut(s) 385
BcoDI GTCTC 1 cut(s) 85
BfaI CTAG 3 cut(s) 440, 804, 1031
BfuAI ACCTGC 1 cut(s) 702
BglII AGATCT 1 cut(s) 907
Bme1390I CCNGG 3 cut(s) 494, 1378, 1429
Bme18I GGWCC 2 cut(s) 134, 855
BmeT110I CYCGRG 1 cut(s) 101
BmgT120I GGNCC 3 cut(s) 134, 480, 855
BmiI GGNNCC 1 cut(s) 1082
BmrFI CCNGG 3 cut(s) 494, 1378, 1429
BmsI GCATC 4 cut(s) 72, 706, 715, 781
BpiI GAAGAC 4 cut(s) 198, 207, 788, 1233
BpmI CTGGAG 2 cut(s) 1399, 1450
Bpu14I TTCGAA 1 cut(s) 960
BpuEI CTTGAG 1 cut(s) 1337
Bsa29I ATCGAT 1 cut(s) 42
BsaAI YACGTR 2 cut(s) 272, 923
BsaBI GATNNNNATC 1 cut(s) 912
BsaJI CCNNGG 4 cut(s) 102, 563, 1108, 1376
BsaWI WCCGGW 1 cut(s) 87
Bsc4I CCNNNNNNNGG 1 cut(s) 477
Bse1I ACTGG 1 cut(s) 1217
Bse3DI GCAATG 1 cut(s) 1106
Bse8I GATNNNNATC 1 cut(s) 912
BseAI TCCGGA 1 cut(s) 87
BseBI CCWGG 3 cut(s) 494, 1378, 1429
BseCI ATCGAT 1 cut(s) 42
BseDI CCNNGG 4 cut(s) 102, 563, 1108, 1376
BseGI GGATG 3 cut(s) 636, 1221, 1263
BseJI GATNNNNATC 1 cut(s) 912
BseLI CCNNNNNNNGG 1 cut(s) 477
BseMI GCAATG 1 cut(s) 1106
BseMII CTCAG 4 cut(s) 306, 336, 490, 498
BseNI ACTGG 1 cut(s) 1217
Bsh1285I CGRYCG 1 cut(s) 826
BshFI GGCC 2 cut(s) 482, 869
BshVI ATCGAT 1 cut(s) 42
BsiEI CGRYCG 1 cut(s) 826
BsiHKAI GWGCWC 3 cut(s) 87, 321, 361
BsiHKCI CYCGRG 1 cut(s) 101
BsiSI CCGG 1 cut(s) 88
BslI CCNNNNNNNGG 1 cut(s) 477
BsmAI GTCTC 1 cut(s) 85
BsmI GAATGC 1 cut(s) 1315
BsnI GGCC 2 cut(s) 482, 869
BsoBI CYCGRG 1 cut(s) 101
Bsp119I TTCGAA 1 cut(s) 960
Bsp1286I GDGCHC 3 cut(s) 87, 321, 361
Bsp13I TCCGGA 1 cut(s) 87
Bsp143I GATC 5 cut(s) 28, 385, 748, 907, 1322
Bsp19I CCATGG 1 cut(s) 1108
BspACI CCGC 5 cut(s) 703, 927, 933, 1268, 1425
BspANI GGCC 2 cut(s) 482, 869
BspCNI CTCAG 4 cut(s) 307, 335, 489, 497
BspDI ATCGAT 1 cut(s) 42
BspEI TCCGGA 1 cut(s) 87
BspHI TCATGA 1 cut(s) 886
BspLI GGNNCC 1 cut(s) 1082
BspMI ACCTGC 1 cut(s) 702
BspT104I TTCGAA 1 cut(s) 960
BsrBI CCGCTC 1 cut(s) 935
BsrDI GCAATG 1 cut(s) 1106
BsrI ACTGG 1 cut(s) 1217
BssECI CCNNGG 4 cut(s) 102, 563, 1108, 1376
BssMI GATC 5 cut(s) 28, 385, 748, 907, 1322
BssNAI GTATAC 1 cut(s) 1335
BssT1I CCWWGG 2 cut(s) 563, 1108
Bst1107I GTATAC 1 cut(s) 1335
Bst2UI CCWGG 3 cut(s) 494, 1378, 1429
Bst4CI ACNGT 4 cut(s) 512, 581, 859, 1232
Bst6I CTCTTC 3 cut(s) 165, 288, 1168
BstBAI YACGTR 2 cut(s) 272, 923
BstBI TTCGAA 1 cut(s) 960
BstC8I GCNNGC 1 cut(s) 361
BstDEI CTNAG 5 cut(s) 315, 322, 476, 484, 547
BstDSI CCRYGG 1 cut(s) 1108
BstF5I GGATG 3 cut(s) 636, 1221, 1263
BstKTI GATC 5 cut(s) 31, 388, 751, 910, 1325
BstMAI GTCTC 1 cut(s) 85
BstMBI GATC 5 cut(s) 28, 385, 748, 907, 1322
BstMCI CGRYCG 1 cut(s) 826
BstMWI GCNNNNNNNGC 1 cut(s) 800
BstNI CCWGG 3 cut(s) 494, 1378, 1429
BstNSI RCATGY 1 cut(s) 1462
BstSCI CCNGG 3 cut(s) 492, 1376, 1427
BstSNI TACGTA 1 cut(s) 923
BstV2I GAAGAC 4 cut(s) 198, 207, 788, 1233
BstX2I RGATCY 1 cut(s) 907
BstXI CCANNNNNNTGG 1 cut(s) 1147
BstYI RGATCY 1 cut(s) 907
BstZ17I GTATAC 1 cut(s) 1335
Bsu15I ATCGAT 1 cut(s) 42
BsuRI GGCC 2 cut(s) 482, 869
BsuTUI ATCGAT 1 cut(s) 42
BtgI CCRYGG 1 cut(s) 1108
BtsCI GGATG 3 cut(s) 636, 1221, 1263
BtsIMutI CAGTG 1 cut(s) 665
BveI ACCTGC 1 cut(s) 702
Cac8I GCNNGC 1 cut(s) 361
CciI TCATGA 1 cut(s) 886
Cfr13I GGNCC 3 cut(s) 134, 480, 855
ClaI ATCGAT 1 cut(s) 42
CsiI ACCWGGT 1 cut(s) 492
Csp6I GTAC 1 cut(s) 924
CviAII CATG 6 cut(s) 505, 533, 887, 1109, 1338, 1459
CviQI GTAC 1 cut(s) 924
DdeI CTNAG 5 cut(s) 315, 322, 476, 484, 547
DpnI GATC 5 cut(s) 30, 387, 750, 909, 1324
DpnII GATC 5 cut(s) 28, 385, 748, 907, 1322
Eam1104I CTCTTC 3 cut(s) 165, 288, 1168
EarI CTCTTC 3 cut(s) 165, 288, 1168
Ecl136II GAGCTC 2 cut(s) 319, 359
Eco105I TACGTA 1 cut(s) 923
Eco130I CCWWGG 2 cut(s) 563, 1108
Eco24I GRGCYC 2 cut(s) 321, 361
Eco47I GGWCC 2 cut(s) 134, 855
Eco53kI GAGCTC 2 cut(s) 319, 359
Eco57I CTGAAG 5 cut(s) 52, 634, 689, 852, 1228
Eco88I CYCGRG 1 cut(s) 101
EcoICRI GAGCTC 2 cut(s) 319, 359
EcoO109I RGGNCCY 1 cut(s) 480
EcoRII CCWGG 3 cut(s) 492, 1376, 1427
EcoT14I CCWWGG 2 cut(s) 563, 1108
EcoT22I ATGCAT 2 cut(s) 65, 1464
EcoT38I GRGCYC 2 cut(s) 321, 361
ErhI CCWWGG 2 cut(s) 563, 1108
FaeI CATG 6 cut(s) 508, 536, 890, 1112, 1341, 1462
FatI CATG 6 cut(s) 504, 532, 886, 1108, 1337, 1458
FauI CCCGC 1 cut(s) 1275
FbaI TGATCA 1 cut(s) 385
FblI GTMKAC 2 cut(s) 861, 1334
FokI GGATG 3 cut(s) 623, 1228, 1250
FriOI GRGCYC 2 cut(s) 321, 361
FspBI CTAG 3 cut(s) 440, 804, 1031
GsuI CTGGAG 2 cut(s) 1399, 1450
HaeIII GGCC 2 cut(s) 482, 869
HapII CCGG 1 cut(s) 88
Hin1II CATG 6 cut(s) 508, 536, 890, 1112, 1341, 1462
HincII GTYRAC 2 cut(s) 300, 1456
HindII GTYRAC 2 cut(s) 300, 1456
HinfI GANTC 7 cut(s) 553, 812, 996, 1027, 1036, 1417, 1432
HpaI GTTAAC 1 cut(s) 1456
HpaII CCGG 1 cut(s) 88
HphI GGTGA 1 cut(s) 1414
Hpy166II GTNNAC 5 cut(s) 300, 464, 862, 1335, 1456
Hpy188I TCNGA 9 cut(s) 71, 134, 325, 456, 487, 823, 1001, 1066, 1437
Hpy8I GTNNAC 5 cut(s) 300, 464, 862, 1335, 1456
HpyAV CCTTC 3 cut(s) 332, 405, 658
HpyCH4III ACNGT 4 cut(s) 512, 581, 859, 1232
HpyCH4IV ACGT 5 cut(s) 81, 120, 271, 922, 1050
HpyCH4V TGCA 6 cut(s) 63, 374, 847, 1091, 1191, 1462
HpyF10VI GCNNNNNNNGC 1 cut(s) 800
HpyF3I CTNAG 5 cut(s) 315, 322, 476, 484, 547
HpySE526I ACGT 5 cut(s) 81, 120, 271, 922, 1050
Hsp92II CATG 6 cut(s) 508, 536, 890, 1112, 1341, 1462
Kpn2I TCCGGA 1 cut(s) 87
Ksp22I TGATCA 1 cut(s) 385
KspAI GTTAAC 1 cut(s) 1456
Kzo9I GATC 5 cut(s) 28, 385, 748, 907, 1322
LmnI GCTCC 1 cut(s) 90
LweI GCATC 4 cut(s) 72, 706, 715, 781
MabI ACCWGGT 1 cut(s) 492
MaeI CTAG 3 cut(s) 440, 804, 1031
MaeII ACGT 5 cut(s) 81, 120, 271, 922, 1050
MaeIII GTNAC 3 cut(s) 310, 1208, 1316
MalI GATC 5 cut(s) 30, 387, 750, 909, 1324
MbiI CCGCTC 1 cut(s) 935
MboI GATC 5 cut(s) 28, 385, 748, 907, 1322
MfeI CAATTG 1 cut(s) 1092
MflI RGATCY 1 cut(s) 907
MhlI GDGCHC 3 cut(s) 87, 321, 361
MluCI AATT 7 cut(s) 261, 956, 1092, 1181, 1203, 1364, 1383
MlyI GAGTC 2 cut(s) 1411, 1441
MmeI TCCRAC 3 cut(s) 247, 846, 1033
MnlI CCTC 9 cut(s) 97, 155, 166, 176, 205, 343, 493, 631, 1166
Mph1103I ATGCAT 2 cut(s) 65, 1464
MroI TCCGGA 1 cut(s) 87
MseI TTAA 3 cut(s) 642, 954, 1455
MslI CAYNNNNRTG 4 cut(s) 18, 413, 723, 1145
MspI CCGG 1 cut(s) 88
MspR9I CCNGG 3 cut(s) 494, 1378, 1429
MunI CAATTG 1 cut(s) 1092
Mva1269I GAATGC 1 cut(s) 1315
MvaI CCWGG 3 cut(s) 494, 1378, 1429
MwoI GCNNNNNNNGC 1 cut(s) 800
NcoI CCATGG 1 cut(s) 1108
NdeII GATC 5 cut(s) 28, 385, 748, 907, 1322
NlaIII CATG 6 cut(s) 508, 536, 890, 1112, 1341, 1462
NlaIV GGNNCC 1 cut(s) 1082
NmeAIII GCCGAG 1 cut(s) 895
NsiI ATGCAT 2 cut(s) 65, 1464
NspI RCATGY 1 cut(s) 1462
NspV TTCGAA 1 cut(s) 960
PagI TCATGA 1 cut(s) 886
PaqCI CACCTGC 1 cut(s) 702
PcsI WCGNNNNNNNCGW 1 cut(s) 157
PctI GAATGC 1 cut(s) 1315
PfeI GAWTC 5 cut(s) 553, 812, 996, 1027, 1036
PleI GAGTC 2 cut(s) 1411, 1440
PpsI GAGTC 2 cut(s) 1411, 1440
Ppu21I YACGTR 2 cut(s) 272, 923
PsiI TTATAA 1 cut(s) 690
Psp124BI GAGCTC 2 cut(s) 321, 361
Psp1406I AACGTT 1 cut(s) 1050
Psp6I CCWGG 3 cut(s) 492, 1376, 1427
PspGI CCWGG 3 cut(s) 492, 1376, 1427
PspN4I GGNNCC 1 cut(s) 1082
PspPI GGNCC 3 cut(s) 134, 480, 855
PsuI RGATCY 1 cut(s) 907
RsaI GTAC 1 cut(s) 925
RsaNI GTAC 1 cut(s) 924
RseI CAYNNNNRTG 4 cut(s) 18, 413, 723, 1145
SacI GAGCTC 2 cut(s) 321, 361
SaqAI TTAA 3 cut(s) 642, 954, 1455
Sau3AI GATC 5 cut(s) 28, 385, 748, 907, 1322
Sau96I GGNCC 3 cut(s) 134, 480, 855
SchI GAGTC 2 cut(s) 1411, 1441
ScrFI CCNGG 3 cut(s) 494, 1378, 1429
SduI GDGCHC 3 cut(s) 87, 321, 361
SexAI ACCWGGT 1 cut(s) 492
SfaNI GCATC 4 cut(s) 72, 706, 715, 781
SfuI TTCGAA 1 cut(s) 960
SinI GGWCC 2 cut(s) 134, 855
SmiMI CAYNNNNRTG 4 cut(s) 18, 413, 723, 1145
SmlI CTYRAG 1 cut(s) 1352
SmoI CTYRAG 1 cut(s) 1352
SnaBI TACGTA 1 cut(s) 923
Sse9I AATT 7 cut(s) 261, 956, 1092, 1181, 1203, 1364, 1383
SsiI CCGC 5 cut(s) 703, 927, 933, 1268, 1425
SspMI CTAG 3 cut(s) 440, 804, 1031
SstI GAGCTC 2 cut(s) 321, 361
StyD4I CCNGG 3 cut(s) 492, 1376, 1427
StyI CCWWGG 2 cut(s) 563, 1108
TaaI ACNGT 4 cut(s) 512, 581, 859, 1232
TaiI ACGT 5 cut(s) 84, 123, 274, 925, 1053
TaqI TCGA 6 cut(s) 42, 151, 411, 960, 994, 1325
TaqII GACCGA 1 cut(s) 840
TasI AATT 7 cut(s) 261, 956, 1092, 1181, 1203, 1364, 1383
TfiI GAWTC 5 cut(s) 553, 812, 996, 1027, 1036
Tru1I TTAA 3 cut(s) 642, 954, 1455
Tru9I TTAA 3 cut(s) 642, 954, 1455
TscAI CASTG 1 cut(s) 672
TspDTI ATGAA 9 cut(s) 273, 290, 351, 397, 509, 630, 875, 903, 1239
TspRI CASTG 1 cut(s) 672
VpaK11BI GGWCC 2 cut(s) 134, 855
XapI RAATTY 4 cut(s) 261, 956, 1364, 1383
XbaI TCTAGA 1 cut(s) 1030
XceI RCATGY 1 cut(s) 1462
XmiI GTMKAC 2 cut(s) 861, 1334
XspI CTAG 3 cut(s) 440, 804, 1031
Zsp2I ATGCAT 2 cut(s) 65, 1464
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.