Rorug04G0423300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0012587
Physical Location & Seq
Forward (+)
58254463 .. 58258866
4404 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0423300.1

Sequence Viewer

Length: 1899 bp
ATGATGAGCTTTGGAGACATTCTCCGATCGGAAAGAGAGAAAGAGATGATGCTCACCACCAGTACTAGTCTCGATCACACCATCGTCGAGAGTGTTATAGCCAGTTTAGGCAAAGGCTTCGATTTAACCTCGGATTTCCGGCTTCAGTATTGTAAAGGCAAACAACGGTTGGTTGCCCTAAGCGACGGCGACAAAAGAGAGCTCGTCGTGCCTGGTTTTGGTTCCATTCCGGACGTCTCTTGCGACATTAAATGCGACAAGGGCGACCGGACGAGGTGCCAGACGGACATGTTCAGCTTCAGTCAGATGTCGGAGTATATCAACCAGAAGTGTAGTGTTCCCGGGAAAGTGCCGTCGGGGTTGTTCAATTCGATGTTCGGGTTCGATAGTGGTTCGTGGGGGAGCGATGCGGCCAAGATAAAATGCTTGGGTGTGGATGGATATTTCATCATCTTCTTCAGTGTTCATATTGATAGGTACCCTCTTGTTCTTGCCGATGAAGTTCGCGATGCTGTTCCGGCCACTTGGGACCCACTTGCCATTTCAAGATTCATAGAAAAATATGGGACGCACATCGTGGTGGGTTTGAGTGTTGGGGGTCAGGATGTGGTGTTGGTTAAGCAAGATAAATCATCAAATTTGGGGCCATCAGACCTCAAAACTCACTTAAACGAGCTTGGAGATCAGCATTTTACGGGGACTTGTAATTTCTCCCCACTCCTCTCCAAAGCAAAAGACCATAAACACAAGGCGCCACAGGCATTCAATGTCTTTGATGCTCCGCAGTCTGTTGCCCTCAACGGCTTCTCCACCGCAAACACAAAAGATGGAATTACTGTGATAAGCTCTAAAAAGGGAGGTGATCCAACGGCCAGTAGCCACAGCGAGTGGCTTCTAACAGTTCCGTCAATGCCGGACGCTATTCAGTACAGCTTCATTCCTATCACTTCTCTCCTTAAAGGTGTTCCCGGCAAAGGTTTCTTGTCCCATGCCATCAACCTCTATCTCAGATACAAGCCTCCTATAGCTGATTTGCCCTACTTTCTTGAATTTCAAGTATCACACAAAGTTTGGGCTCCCCTTCACAATGACCTACGTTTAGGTCCTGCCACAAATAGCAAATCTAATCCATCATCACCTGCTATTCACTTCAGCTTGATGGGTCCTAGGTTATATGTAAACACCACACAGGTTAGAGTTGGAAATAATGCCCCTGTCACAGGGATGCGCTTGTTTCTTGAGGGCATGAAATGCAACAGGCTAGCCATCCACCTCCAACATCTAGCAAACACTCCATTGATGCTGGAGAACAAGATCGATGACACAACGATGTGGCGAGGATCAGAAGAGATCGACGATGACCGGTACTTTGAAGCAACAAACGGGAAAAAGTTCTCCCACGTGTGCACGGCGCCAGTAACGTACGACCCAAAATGGATATCTTCGGGCATAACCTCGGCCTTCATTGTCTCGGGAGCACAACTTGTGGTCAAGAAACACGGCTCAAAGAATGTTCTCCATCTTCGGCTCTTGTTCTCCAAAGTCTTGGACTCTTTCTTAGTGCAATCAAATTGGGCAGAGTCATGTTCTTCAACTAAATCGTCGTCGTCTGGCCTTTTGAATGCGATGAGTAGACCAATTTCTGGGAACTATTGGGCAAAAGAGAAAGTAGAACCTGCCGCCGAGGATGTGATATTGGATTCGAGTGTTTTTCCTGCAGGGCCTCCGGTGCCTTTGCCAAGGCAAAAATTTGTGAAGCATGTAAACACCTCACATGTATGCAAAGGGCCGCAGGATAGTCCTGGCCATTGGTTAGTCACCGGAGCAAAGCTCCACTTGGTCAAGGGAAATATTGGCTTGCAAGTCAAGTTCTCTCTGTTAAACCTAGCAGAAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

632

Amino Acids

69.31

Weight (kDa)

8.66

Isoelectric Point (pI)

36.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MACPF PF01823 121 - 335 8.8e-29 MAC/Perforin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1147
AatII GACGTC 1 cut(s) 237
Acc36I ACCTGC 2 cut(s) 1147, 1684
Acc65I GGTACC 1 cut(s) 477
AccB1I GGYRCC 5 cut(s) 276, 477, 751, 1411, 1729
AccB7I CCANNNNNTGG 1 cut(s) 1643
AccI GTMKAC 1 cut(s) 1633
AccII CGCG 1 cut(s) 507
AccIII TCCGGA 1 cut(s) 229
AciI CCGC 5 cut(s) 410, 782, 813, 1680, 1790
AclWI GGATC 2 cut(s) 857, 1348
AcoI YGGCCR 4 cut(s) 411, 519, 870, 1804
AcsI RAATTY 3 cut(s) 637, 1049, 1748
AcuI CTGAAG 4 cut(s) 128, 283, 442, 1135
AcvI CACGTG 1 cut(s) 1402
AcyI GRCGYC 3 cut(s) 234, 752, 1412
AdeI CACNNNGTG 1 cut(s) 577
AfaI GTAC 5 cut(s) 64, 479, 929, 1367, 1424
AfiI CCNNNNNNNGG 4 cut(s) 218, 974, 1220, 1643
AflIII ACRYGT 3 cut(s) 288, 1401, 1774
AgeI ACCGGT 1 cut(s) 1362
AgsI TTSAA 8 cut(s) 367, 546, 766, 1049, 1055, 1373, 1593, 1621
AhlI ACTAGT 1 cut(s) 65
AjnI CCWGG 2 cut(s) 211, 1801
AluBI AGCT 9 cut(s) 9, 202, 297, 676, 846, 933, 1028, 1155, 1831
AluI AGCT 9 cut(s) 9, 202, 297, 676, 846, 933, 1028, 1155, 1831
Alw21I GWGCWC 3 cut(s) 204, 1409, 1480
Alw26I GTCTC 4 cut(s) 9, 74, 241, 1474
Alw44I GTGCAC 1 cut(s) 1405
AlwI GGATC 2 cut(s) 857, 1348
Ama87I CYCGRG 2 cut(s) 341, 1471
Aor13HI TCCGGA 1 cut(s) 229
AoxI GGCC 9 cut(s) 411, 519, 644, 870, 1458, 1612, 1721, 1787, 1804
ApaLI GTGCAC 1 cut(s) 1405
ApoI RAATTY 3 cut(s) 637, 1049, 1748
ArsI GACNNNNNNTTYG 2 cut(s) 1352, 1384
AsiGI ACCGGT 1 cut(s) 1362
Asp700I GAANNNNTTC 1 cut(s) 1391
Asp718I GGTACC 1 cut(s) 477
AspA2I CCTAGG 1 cut(s) 1166
AspLEI GCGC 3 cut(s) 754, 1230, 1414
AspS9I GGNCC 6 cut(s) 529, 644, 1103, 1163, 1721, 1787
AsuC2I CCSGG 3 cut(s) 342, 343, 969
AsuHPI GGTGA 4 cut(s) 46, 872, 1128, 1810
AsuNHI GCTAGC 1 cut(s) 1261
AvaI CYCGRG 2 cut(s) 341, 1471
AvaII GGWCC 3 cut(s) 529, 1103, 1163
AvrII CCTAGG 1 cut(s) 1166
BaeGI GKGCMC 1 cut(s) 1409
BalI TGGCCA 1 cut(s) 1806
BanI GGYRCC 5 cut(s) 276, 477, 751, 1411, 1729
BanII GRGCYC 2 cut(s) 204, 1078
BbrPI CACGTG 1 cut(s) 1402
Bbv12I GWGCWC 3 cut(s) 204, 1409, 1480
BccI CCATC 9 cut(s) 89, 431, 655, 821, 1001, 1138, 1153, 1274, 1527
BceAI ACGGC 6 cut(s) 202, 337, 817, 885, 1425, 1516
BcgI CGANNNNNNTGC 2 cut(s) 100, 134
BciT130I CCWGG 2 cut(s) 213, 1803
BcnI CCSGG 3 cut(s) 342, 343, 969
BcoDI GTCTC 4 cut(s) 9, 74, 241, 1474
BcuI ACTAGT 1 cut(s) 65
BfaI CTAG 5 cut(s) 66, 1167, 1262, 1283, 1886
BfmI CTRYAG 2 cut(s) 1023, 1716
BfoI RGCGCY 2 cut(s) 755, 1415
BfuAI ACCTGC 2 cut(s) 1147, 1684
BisI GCNGC 3 cut(s) 411, 1680, 1790
BlnI CCTAGG 1 cut(s) 1166
BlsI GCNGC 3 cut(s) 412, 1681, 1791
BmcAI AGTACT 1 cut(s) 64
Bme1390I CCNGG 5 cut(s) 213, 342, 343, 969, 1803
Bme18I GGWCC 3 cut(s) 529, 1103, 1163
BmeT110I CYCGRG 2 cut(s) 341, 1471
BmgT120I GGNCC 6 cut(s) 529, 644, 1103, 1163, 1721, 1787
BmrFI CCNGG 5 cut(s) 213, 342, 343, 969, 1803
BmsI GCATC 6 cut(s) 39, 397, 499, 766, 1215, 1290
BmtI GCTAGC 1 cut(s) 1265
BplI GAGNNNNNCTC 3 cut(s) 38, 1815, 1847
BpmI CTGGAG 1 cut(s) 1325
Bpu10I CCTNAGC 1 cut(s) 179
BpuEI CTTGAG 1 cut(s) 1259
BpuMI CCSGG 3 cut(s) 342, 343, 969
Bsa29I ATCGAT 1 cut(s) 1317
BsaAI YACGTR 1 cut(s) 1402
BsaHI GRCGYC 3 cut(s) 234, 752, 1412
BsaJI CCNNGG 6 cut(s) 129, 341, 1166, 1455, 1683, 1739
BsaWI WCCGGW 5 cut(s) 229, 267, 1362, 1726, 1820
BsaXI ACNNNNNCTCC 2 cut(s) 791, 821
Bsc4I CCNNNNNNNGG 4 cut(s) 218, 974, 1220, 1643
Bse118I RCCGGY 1 cut(s) 1362
Bse1I ACTGG 4 cut(s) 60, 102, 873, 1415
BseAI TCCGGA 1 cut(s) 229
BseBI CCWGG 2 cut(s) 213, 1803
BseCI ATCGAT 1 cut(s) 1317
BseDI CCNNGG 6 cut(s) 129, 341, 1166, 1455, 1683, 1739
BseGI GGATG 5 cut(s) 442, 610, 1230, 1266, 1693
BseLI CCNNNNNNNGG 4 cut(s) 218, 974, 1220, 1643
BseMII CTCAG 1 cut(s) 1021
BseNI ACTGG 4 cut(s) 60, 102, 873, 1415
BseRI GAGGAG 1 cut(s) 710
BseSI GKGCMC 1 cut(s) 1409
Bsh1236I CGCG 1 cut(s) 507
Bsh1285I CGRYCG 2 cut(s) 29, 268
BshFI GGCC 9 cut(s) 413, 521, 646, 872, 1460, 1614, 1723, 1789, 1806
BshNI GGYRCC 5 cut(s) 276, 477, 751, 1411, 1729
BshTI ACCGGT 1 cut(s) 1362
BshVI ATCGAT 1 cut(s) 1317
BsiEI CGRYCG 2 cut(s) 29, 268
BsiHKAI GWGCWC 3 cut(s) 204, 1409, 1480
BsiHKCI CYCGRG 2 cut(s) 341, 1471
BsiWI CGTACG 1 cut(s) 1422
BslFI GGGAC 4 cut(s) 542, 580, 712, 970
BslI CCNNNNNNNGG 4 cut(s) 218, 974, 1220, 1643
BsmAI GTCTC 4 cut(s) 9, 74, 241, 1474
BsmBI CGTCTC 1 cut(s) 241
BsmFI GGGAC 4 cut(s) 542, 580, 712, 970
BsmI GAATGC 2 cut(s) 761, 1627
BsnI GGCC 9 cut(s) 413, 521, 646, 872, 1460, 1614, 1723, 1789, 1806
BsoBI CYCGRG 2 cut(s) 341, 1471
Bsp1286I GDGCHC 4 cut(s) 204, 1078, 1409, 1480
Bsp13I TCCGGA 1 cut(s) 229
Bsp143I GATC 7 cut(s) 26, 73, 682, 862, 1314, 1340, 1350
Bsp68I TCGCGA 1 cut(s) 507
BspACI CCGC 5 cut(s) 410, 782, 813, 1680, 1790
BspANI GGCC 9 cut(s) 413, 521, 646, 872, 1460, 1614, 1723, 1789, 1806
BspCNI CTCAG 1 cut(s) 1020
BspDI ATCGAT 1 cut(s) 1317
BspEI TCCGGA 1 cut(s) 229
BspFNI CGCG 1 cut(s) 507
BspMAI CTGCAG 1 cut(s) 1720
BspMI ACCTGC 2 cut(s) 1147, 1684
BspOI GCTAGC 1 cut(s) 1265
BspPI GGATC 2 cut(s) 857, 1348
BspT107I GGYRCC 5 cut(s) 276, 477, 751, 1411, 1729
BsrFI RCCGGY 1 cut(s) 1362
BsrI ACTGG 4 cut(s) 60, 102, 873, 1415
BssAI RCCGGY 1 cut(s) 1362
BssECI CCNNGG 6 cut(s) 129, 341, 1166, 1455, 1683, 1739
BssMI GATC 7 cut(s) 26, 73, 682, 862, 1314, 1340, 1350
BssNI GRCGYC 3 cut(s) 234, 752, 1412
BssT1I CCWWGG 2 cut(s) 1166, 1739
Bst2UI CCWGG 2 cut(s) 213, 1803
Bst4CI ACNGT 3 cut(s) 168, 838, 901
Bst6I CTCTTC 1 cut(s) 1341
BstACI GRCGYC 3 cut(s) 234, 752, 1412
BstAPI GCANNNNNTGC 1 cut(s) 1251
BstBAI YACGTR 1 cut(s) 1402
BstC8I GCNNGC 2 cut(s) 1263, 1859
BstDEI CTNAG 3 cut(s) 179, 1007, 1558
BstENI CCTNNNNNAGG 1 cut(s) 1218
BstF5I GGATG 5 cut(s) 442, 610, 1230, 1266, 1693
BstFNI CGCG 1 cut(s) 507
BstH2I RGCGCY 2 cut(s) 755, 1415
BstHHI GCGC 3 cut(s) 754, 1230, 1414
BstKTI GATC 7 cut(s) 29, 76, 685, 865, 1317, 1343, 1353
BstMAI GTCTC 4 cut(s) 9, 74, 241, 1474
BstMBI GATC 7 cut(s) 26, 73, 682, 862, 1314, 1340, 1350
BstMCI CGRYCG 2 cut(s) 29, 268
BstMWI GCNNNNNNNGC 6 cut(s) 208, 261, 518, 758, 1251, 1729
BstNI CCWGG 2 cut(s) 213, 1803
BstNSI RCATGY 3 cut(s) 292, 1763, 1778
BstSCI CCNGG 5 cut(s) 211, 340, 341, 967, 1801
BstSFI CTRYAG 2 cut(s) 1023, 1716
BstSLI GKGCMC 1 cut(s) 1409
BstUI CGCG 1 cut(s) 507
BstXI CCANNNNNNTGG 1 cut(s) 1546
Bsu15I ATCGAT 1 cut(s) 1317
BsuRI GGCC 9 cut(s) 413, 521, 646, 872, 1460, 1614, 1723, 1789, 1806
BsuTUI ATCGAT 1 cut(s) 1317
BtgZI GCGATG 3 cut(s) 420, 522, 1640
BtsCI GGATG 5 cut(s) 442, 610, 1230, 1266, 1693
BtsIMutI CAGTG 1 cut(s) 466
BtuMI TCGCGA 1 cut(s) 507
BveI ACCTGC 2 cut(s) 1147, 1684
Cac8I GCNNGC 2 cut(s) 1263, 1859
CfoI GCGC 3 cut(s) 754, 1230, 1414
Cfr10I RCCGGY 1 cut(s) 1362
Cfr13I GGNCC 6 cut(s) 529, 644, 1103, 1163, 1721, 1787
Cfr9I CCCGGG 1 cut(s) 341
ClaI ATCGAT 1 cut(s) 1317
CseI GACGC 2 cut(s) 577, 926
Csp6I GTAC 5 cut(s) 63, 478, 928, 1366, 1423
CspAI ACCGGT 1 cut(s) 1362
CviAII CATG 6 cut(s) 289, 989, 1246, 1584, 1760, 1775
CviQI GTAC 5 cut(s) 63, 478, 928, 1366, 1423
DdeI CTNAG 3 cut(s) 179, 1007, 1558
DinI GGCGCC 2 cut(s) 753, 1413
DpnI GATC 7 cut(s) 28, 75, 684, 864, 1316, 1342, 1352
DpnII GATC 7 cut(s) 26, 73, 682, 862, 1314, 1340, 1350
DraIII CACNNNGTG 1 cut(s) 577
EaeI YGGCCR 4 cut(s) 411, 519, 870, 1804
Eam1104I CTCTTC 1 cut(s) 1341
EarI CTCTTC 1 cut(s) 1341
Ecl136II GAGCTC 1 cut(s) 202
Eco130I CCWWGG 2 cut(s) 1166, 1739
Eco24I GRGCYC 2 cut(s) 204, 1078
Eco32I GATATC 1 cut(s) 1440
Eco47I GGWCC 3 cut(s) 529, 1103, 1163
Eco53kI GAGCTC 1 cut(s) 202
Eco57I CTGAAG 4 cut(s) 128, 283, 442, 1135
Eco72I CACGTG 1 cut(s) 1402
Eco88I CYCGRG 2 cut(s) 341, 1471
EcoICRI GAGCTC 1 cut(s) 202
EcoNI CCTNNNNNAGG 1 cut(s) 1218
EcoO109I RGGNCCY 4 cut(s) 529, 1103, 1163, 1721
EcoRII CCWGG 2 cut(s) 211, 1801
EcoRV GATATC 1 cut(s) 1440
EcoT14I CCWWGG 2 cut(s) 1166, 1739
EcoT38I GRGCYC 2 cut(s) 204, 1078
EgeI GGCGCC 2 cut(s) 753, 1413
EheI GGCGCC 2 cut(s) 753, 1413
ErhI CCWWGG 2 cut(s) 1166, 1739
Esp3I CGTCTC 1 cut(s) 241
FaeI CATG 6 cut(s) 292, 992, 1249, 1587, 1763, 1778
FalI AAGNNNNNCTT 2 cut(s) 1820, 1852
FaqI GGGAC 4 cut(s) 542, 580, 712, 970
FatI CATG 6 cut(s) 288, 988, 1245, 1583, 1759, 1774
FblI GTMKAC 1 cut(s) 1633
Fnu4HI GCNGC 3 cut(s) 411, 1680, 1790
FokI GGATG 5 cut(s) 449, 617, 1237, 1253, 1700
FriOI GRGCYC 2 cut(s) 204, 1078
Fsp4HI GCNGC 3 cut(s) 411, 1680, 1790
FspBI CTAG 5 cut(s) 66, 1167, 1262, 1283, 1886
GlaI GCGC 3 cut(s) 753, 1229, 1413
GluI GCNGC 3 cut(s) 411, 1680, 1790
GsuI CTGGAG 1 cut(s) 1325
HaeII RGCGCY 2 cut(s) 755, 1415
HaeIII GGCC 9 cut(s) 413, 521, 646, 872, 1460, 1614, 1723, 1789, 1806
HgaI GACGC 2 cut(s) 577, 926
HhaI GCGC 3 cut(s) 754, 1230, 1414
Hin1I GRCGYC 3 cut(s) 234, 752, 1412
Hin1II CATG 6 cut(s) 292, 992, 1249, 1587, 1763, 1778
Hin6I GCGC 3 cut(s) 752, 1228, 1412
HinP1I GCGC 3 cut(s) 752, 1228, 1412
HinfI GANTC 4 cut(s) 549, 1550, 1580, 1700
HphI GGTGA 4 cut(s) 46, 872, 1128, 1810
Hpy166II GTNNAC 4 cut(s) 1180, 1407, 1634, 1765
Hpy188I TCNGA 8 cut(s) 26, 31, 133, 306, 313, 652, 1010, 1345
Hpy8I GTNNAC 4 cut(s) 1180, 1407, 1634, 1765
Hpy99I CGWCG 7 cut(s) 89, 188, 209, 358, 1358, 1606, 1609
HpyAV CCTTC 2 cut(s) 1091, 1471
HpyCH4III ACNGT 3 cut(s) 168, 838, 901
HpyCH4IV ACGT 4 cut(s) 234, 1096, 1401, 1421
HpyCH4V TGCA 6 cut(s) 1254, 1407, 1564, 1718, 1782, 1861
HpyF10VI GCNNNNNNNGC 6 cut(s) 208, 261, 518, 758, 1251, 1729
HpyF3I CTNAG 3 cut(s) 179, 1007, 1558
HpySE526I ACGT 4 cut(s) 234, 1096, 1401, 1421
Hsp92I GRCGYC 3 cut(s) 234, 752, 1412
Hsp92II CATG 6 cut(s) 292, 992, 1249, 1587, 1763, 1778
HspAI GCGC 3 cut(s) 752, 1228, 1412
KasI GGCGCC 2 cut(s) 751, 1411
KflI GGGWCCC 1 cut(s) 529
Kpn2I TCCGGA 1 cut(s) 229
KpnI GGTACC 1 cut(s) 481
Kzo9I GATC 7 cut(s) 26, 73, 682, 862, 1314, 1340, 1350
LmnI GCTCC 6 cut(s) 402, 784, 1081, 1475, 1823, 1836
LweI GCATC 6 cut(s) 39, 397, 499, 766, 1215, 1290
MaeI CTAG 5 cut(s) 66, 1167, 1262, 1283, 1886
MaeII ACGT 4 cut(s) 234, 1096, 1401, 1421
MaeIII GTNAC 3 cut(s) 1216, 1417, 1816
MalI GATC 7 cut(s) 28, 75, 684, 864, 1316, 1342, 1352
MboI GATC 7 cut(s) 26, 73, 682, 862, 1314, 1340, 1350
MboII GAAGA 6 cut(s) 445, 448, 1358, 1434, 1514, 1581
MhlI GDGCHC 4 cut(s) 204, 1078, 1409, 1480
MlsI TGGCCA 1 cut(s) 1806
MluCI AATT 8 cut(s) 367, 637, 706, 831, 1049, 1570, 1638, 1748
MluNI TGGCCA 1 cut(s) 1806
Mly113I GGCGCC 2 cut(s) 752, 1412
MlyI GAGTC 2 cut(s) 1544, 1589
MmeI TCCRAC 4 cut(s) 291, 890, 1180, 1300
Mox20I TGGCCA 1 cut(s) 1806
MroI TCCGGA 1 cut(s) 229
MroXI GAANNNNTTC 1 cut(s) 1391
MscI TGGCCA 1 cut(s) 1806
MseI TTAA 6 cut(s) 125, 249, 618, 668, 957, 1880
MslI CAYNNNNRTG 4 cut(s) 578, 1223, 1328, 1777
Msp20I TGGCCA 1 cut(s) 1806
MspR9I CCNGG 5 cut(s) 213, 342, 343, 969, 1803
Mva1269I GAATGC 2 cut(s) 761, 1627
MvaI CCWGG 2 cut(s) 213, 1803
MvnI CGCG 1 cut(s) 507
MwoI GCNNNNNNNGC 6 cut(s) 208, 261, 518, 758, 1251, 1729
NarI GGCGCC 2 cut(s) 752, 1412
NciI CCSGG 3 cut(s) 342, 343, 969
NdeII GATC 7 cut(s) 26, 73, 682, 862, 1314, 1340, 1350
NheI GCTAGC 1 cut(s) 1261
NlaIII CATG 6 cut(s) 292, 992, 1249, 1587, 1763, 1778
NmeAIII GCCGAG 2 cut(s) 1436, 1708
NmuCI GTSAC 2 cut(s) 1216, 1816
NruI TCGCGA 1 cut(s) 507
NspI RCATGY 3 cut(s) 292, 1763, 1778
PaqCI CACCTGC 1 cut(s) 1147
PciI ACATGT 2 cut(s) 288, 1774
PcsI WCGNNNNNNNCGW 1 cut(s) 240
PctI GAATGC 2 cut(s) 761, 1627
PdmI GAANNNNTTC 1 cut(s) 1391
PfeI GAWTC 2 cut(s) 549, 1700
Pfl23II CGTACG 1 cut(s) 1422
PflMI CCANNNNNTGG 1 cut(s) 1643
PinAI ACCGGT 1 cut(s) 1362
PkrI GCNGC 3 cut(s) 412, 1681, 1791
Ple19I CGATCG 1 cut(s) 29
PleI GAGTC 2 cut(s) 1544, 1588
PluTI GGCGCC 2 cut(s) 755, 1415
PmaCI CACGTG 1 cut(s) 1402
PmlI CACGTG 1 cut(s) 1402
PpsI GAGTC 2 cut(s) 1544, 1588
Ppu21I YACGTR 1 cut(s) 1402
PpuMI RGGWCCY 3 cut(s) 529, 1103, 1163
PscI ACATGT 2 cut(s) 288, 1774
Psp124BI GAGCTC 1 cut(s) 204
Psp5II RGGWCCY 3 cut(s) 529, 1103, 1163
Psp6I CCWGG 2 cut(s) 211, 1801
PspCI CACGTG 1 cut(s) 1402
PspGI CCWGG 2 cut(s) 211, 1801
PspLI CGTACG 1 cut(s) 1422
PspPI GGNCC 6 cut(s) 529, 644, 1103, 1163, 1721, 1787
PspPPI RGGWCCY 3 cut(s) 529, 1103, 1163
PstI CTGCAG 1 cut(s) 1720
PvuI CGATCG 1 cut(s) 29
RruI TCGCGA 1 cut(s) 507
RsaI GTAC 5 cut(s) 64, 479, 929, 1367, 1424
RsaNI GTAC 5 cut(s) 63, 478, 928, 1366, 1423
RseI CAYNNNNRTG 4 cut(s) 578, 1223, 1328, 1777
SacI GAGCTC 1 cut(s) 204
SaqAI TTAA 6 cut(s) 125, 249, 618, 668, 957, 1880
SatI GCNGC 3 cut(s) 411, 1680, 1790
Sau3AI GATC 7 cut(s) 26, 73, 682, 862, 1314, 1340, 1350
Sau96I GGNCC 6 cut(s) 529, 644, 1103, 1163, 1721, 1787
SbfI CCTGCAGG 1 cut(s) 1720
ScaI AGTACT 1 cut(s) 64
SchI GAGTC 2 cut(s) 1544, 1589
ScrFI CCNGG 5 cut(s) 213, 342, 343, 969, 1803
SdaI CCTGCAGG 1 cut(s) 1720
SduI GDGCHC 4 cut(s) 204, 1078, 1409, 1480
SfaNI GCATC 6 cut(s) 39, 397, 499, 766, 1215, 1290
SfcI CTRYAG 2 cut(s) 1023, 1716
SfoI GGCGCC 2 cut(s) 753, 1413
SinI GGWCC 3 cut(s) 529, 1103, 1163
SmaI CCCGGG 1 cut(s) 343
SmiMI CAYNNNNRTG 4 cut(s) 578, 1223, 1328, 1777
SmlI CTYRAG 1 cut(s) 1238
SmoI CTYRAG 1 cut(s) 1238
SpeI ACTAGT 1 cut(s) 65
Sse8387I CCTGCAGG 1 cut(s) 1720
Sse9I AATT 8 cut(s) 367, 637, 706, 831, 1049, 1570, 1638, 1748
SsiI CCGC 5 cut(s) 410, 782, 813, 1680, 1790
SspDI GGCGCC 2 cut(s) 751, 1411
SspI AATATT 1 cut(s) 1852
SspMI CTAG 5 cut(s) 66, 1167, 1262, 1283, 1886
SstI GAGCTC 1 cut(s) 204
StyD4I CCNGG 5 cut(s) 211, 340, 341, 967, 1801
StyI CCWWGG 2 cut(s) 1166, 1739
TaaI ACNGT 3 cut(s) 168, 838, 901
TaiI ACGT 4 cut(s) 237, 1099, 1404, 1424
TaqI TCGA 8 cut(s) 72, 87, 120, 371, 384, 1317, 1353, 1703
TasI AATT 8 cut(s) 367, 637, 706, 831, 1049, 1570, 1638, 1748
TatI WGTACW 2 cut(s) 62, 927
TauI GCSGC 3 cut(s) 413, 1682, 1792
TfiI GAWTC 2 cut(s) 549, 1700
Tru1I TTAA 6 cut(s) 125, 249, 618, 668, 957, 1880
Tru9I TTAA 6 cut(s) 125, 249, 618, 668, 957, 1880
TscAI CASTG 1 cut(s) 466
TseFI GTSAC 2 cut(s) 1216, 1816
Tsp45I GTSAC 2 cut(s) 1216, 1816
TspDTI ATGAA 7 cut(s) 436, 455, 513, 541, 925, 1262, 1453
TspGWI ACGGA 2 cut(s) 299, 894
TspMI CCCGGG 1 cut(s) 341
TspRI CASTG 1 cut(s) 466
Van91I CCANNNNNTGG 1 cut(s) 1643
VneI GTGCAC 1 cut(s) 1405
VpaK11BI GGWCC 3 cut(s) 529, 1103, 1163
XagI CCTNNNNNAGG 1 cut(s) 1218
XapI RAATTY 3 cut(s) 637, 1049, 1748
XceI RCATGY 3 cut(s) 292, 1763, 1778
XmaI CCCGGG 1 cut(s) 341
XmaJI CCTAGG 1 cut(s) 1166
XmiI GTMKAC 1 cut(s) 1633
XmnI GAANNNNTTC 1 cut(s) 1391
XspI CTAG 5 cut(s) 66, 1167, 1262, 1283, 1886
ZraI GACGTC 1 cut(s) 235
ZrmI AGTACT 1 cut(s) 64
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.