Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000004

Orthogroup ID

OG0015393

Physical Location & Seq

Genomic Coordinates Reverse (-)

12530731 .. 12538697

Gene Structure 7967 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug04G0082000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2493 bp

ATGGGTCAGCCGGCGCCTCGTCCCCAAGCCGCTGTTCCTGAGGCGGCCGTTCCTGAAGCGGTCAGCGCTGAAGCGGCCCGGTGCCGAGGACTTGTCGGCGGCGGCGGCGGCGGAGGACGACGAGGGGCCCAAGCCACCGCTCTCTTCGCCTTCTCACTCTCTGCCTCTGTTGGTACTTCTAACGACATTGTCGCGGAAACCTTAGCTAATGGAGACGAGGAGGAGGAGCCTCCCGACGGCGCCCTTGATGATGCCCAATCACGTACAGTTATTGGATTGTGGAAAGTCAATCTGGAAGAACCCAAATCTCACCTGTCTGATCCGTCGGTTATAATTCATGATATCTCCACAAAAACACGCATTAGACTTGCATCTGCAAATGAAAACGACAAGCAACAATTTCAGTGGTTTAGCAATGCATGCAAAGGAATTCCACAGGCACGACCTGCATTTTTGGGTAATAACAATCCAGCAACTGCACCACCAACAACTGCAGAAGATATAGAGTTGGCCGTGGCTCTCAGCTCCTCCCTTCATTCTGCTTCACAGGAGAGACCACACTCAAGTAGCTCCGGTACCTCTACAAATGCTTTTAACTTGAATGGTTGGAGTACATCCACAAACACTGGCAACTACAACGGTTGGGATGCAACAATTGCAGCTGCTTCTCCAAAAGTACGTAGCAGCGACTGGTCAGGGGTTCAAACTGGCCTAGTTGGTGACTCAATTGGACATGCAAACATCCAGGAAACCTCTCCTACCCAAACAACTCCTACCTCTGATTCAGTTATATCATCTTCAACACAATCAGCTGCAATCCCATCGGCTCCACCAGTTTTAGATGAAAGTCTAGATGAATATCCTATCCACTATCCATCAATTGATGACAGCCCTATTGATCTATCAAACCCAACTGCTGAAAGTATACCTCCTACAGTTGATGAAAAGAAAGGTGATGGGGCCTCTTCTTCATGTGTGATTTGTTTAGATGCACCAGTTGAAGGAGCTTGCATCCCATGTGGCCATATGGCTGGATGCATGTCTTGTTTGACTGAGATTAAAGGTAAGAAATGGGGCTGTCCTGTCTGCCGAGCCAAGAATGAACAGGTTGTAAGGCTCTATGCTAGTCATGCCAGTCAACCAGACTATTACTACAACTTGAATACCATTCTTCGAATCTGTTCGTCTCCAATAACAGATGAAAAGATTGTTTCTTGTGTGGAAAGAAAAAAGAACAGAAGGATGGGGTCGTCTTCCTCCCAGAGAATCAACATTATTCAGAAACTCGAAGTCGATAACCGTTTTCCTCTGCGTTTTTACTTTCGGATGGCTGATATATTTCTCAGACAGGCTGACATATATCGGGAAGAGAAAGATATTTTTTACCTGTACATTACGCTCCTAAGGTTTGCAAGTTTGGTCTCCGAAACAATACCGGATCATCAAGACTATATAAAGGCTGATCAAAGAGAAAGATCCTCTCAGAAGGAAGTGAAAAAGAAATTGTTGAATGCTCTTGCTGAGCTAGAGGAGTTAAAACCAGCAGTTAAACAGAAGATCAATGAGATAAACAGAGGAAATGCATATCAAAACAGCAGGCATGATCACCAAACTGGGGAAAGTATACCTCGTAGATCTGATAAAAAGAAAGTCGAAAGTGTGGGGACTTGTAGCTCCAGTACTGGTGGAGAGAATTTATCCCCACACCCAACACCAGAGTTTCCCTTTGGTCTCCGTGGTAACAAGAGATTAAAGAAAGAGACACGAACAAAAATTTTACTTGAAGAGGCACATGATTGGAAGCTTACCACTCCATGCAAGCCAATACCACAGGCGCATCCTGCAATTAACCAGCCAACTGCACCACGAGTTGAAGAAGATTTAGAGCTGGTCAAGGCTATTGATGCCTCCATCCAGTCTGCTTTGCAGGAGAGGCCGCCCTTGTCTGATGCACTCCGAATCCATGAAACAATTGCATCAAGTAGTTCGAGTACCTCCATGGATAGTGGAGACTTGTCACAGTGTGAAAGTGTCCCAGGAGGGGATTCAGATTCACATGCAGAGATCCATGTTTTTTTTTTTAAGAAAGTAAGAATATTATTAGATACGATTAAAATCGTACATGAGGGCATCCAGCACAAATTCTGGAGCATGAGTAAACCATTCAAGATCCATGTTACCTCTACCATTCAAACATCTACTACCTCAGATTCAATCCCATCATCTCCAACCCCATCAGCTCCACCAGTTTCAGATGATTGTCTAAATGATCCTTTTCAGTGTCGATCAACTGATTCCAGTCCTATCCAGGAAGGTGAAAATGCTTCTTCTTCATGTGTGATATGTATGGATGCTCCAGTTGAAGGAGCTTTTATCCCATGTGGGCATATGGCTGGATGCATGCCTTGTTTGAATGACATTAAAGATAAGAACTGGCATTGCCCTGTCTGCCGCGCTAAGGTCGATCAGGTCATAAAGCTCTATGCTGTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004601 GO:0005575 GO:0005618 GO:0005623 GO:0005911 GO:0008150 GO:0008152 GO:0009505 GO:0009506 GO:0009636 GO:0009664 GO:0009987 GO:0016043 GO:0016209 GO:0016491 GO:0016684 GO:0030054 GO:0030312 GO:0042221 GO:0044464 GO:0045229 GO:0050896 GO:0051716 GO:0055044 GO:0055114 GO:0070887 GO:0071554 GO:0071555 GO:0071669 GO:0071840 GO:0071944 GO:0097237 GO:0098754 GO:0098869 GO:1990748

KEGG Pathways

Metabolic & Signaling

ko00940 ko01100 ko01110 map00940 map01100 map01110

Pfam Domains

Protein Families

peroxidase

Protein Analysis

830

Amino Acids

89.99

Weight (kDa)

5.71

Isoelectric Point (pI)

54.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-C3HC4_3	PF13920	323 - 369	6.9e-12	Zinc finger, C3HC4 type (RING finger)
USP8_dimer	PF08969	426 - 515	1.1e-09	USP8 dimerisation domain
zf-C3HC4_3	PF13920	777 - 824	9.4e-14	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0015393)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G43480 AT5G24070
fragaria_vesca	FvH4_3g26850
malus_domestica	MD04G1046400.v1.1
prunus_persica	Prupe.1G199900_v2.0.a1
rosa_chinensis	RchiOBHm_Chr4g0407891
rosa_laevigata	RLG00000008691
rosa_multiflora	Rmu_co8108400.1_g000001 Rmu_sc0029075.1_g000001
rosa_roxburghii	Rroxscaffold_5G00352430
rosa_rugosa	Rorug04G0082000
rosa_samantha	Rh4AG147100 Rh4BG144600 Rh4CG153900 Rh4DG141100
rosa_wichuraiana	Rw4G012080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	332
Acc36I	ACCTGC	1 cut(s)	454
Acc65I	GGTACC	1 cut(s)	575
AccB1I	GGYRCC	4 cut(s)	13, 81, 239, 575
AccBSI	CCGCTC	1 cut(s)	140
AccI	GTMKAC	2 cut(s)	925, 1624
AccII	CGCG	2 cut(s)	194, 2454
AclWI	GGATC	6 cut(s)	314, 1446, 1470, 2059, 2164, 2264
AcoI	YGGCCR	3 cut(s)	45, 510, 1021
AcsI	RAATTY	4 cut(s)	429, 1693, 1773, 2141
AcuI	CTGAAG	2 cut(s)	75, 90
AcyI	GRCGYC	2 cut(s)	14, 240
AdeI	CACNNNGTG	1 cut(s)	2024
AfaI	GTAC	9 cut(s)	175, 265, 577, 613, 678, 1391, 1681, 1993, 2121
AfeI	AGCGCT	1 cut(s)	67
AfiI	CCNNNNNNNGG	6 cut(s)	236, 1001, 1615, 2041, 2363, 2458
AjnI	CCWGG	3 cut(s)	744, 2035, 2307
AjuI	GAANNNNNNNTTGG	2 cut(s)	18, 50
Alw26I	GTCTC	7 cut(s)	207, 547, 1191, 1426, 1736, 1754, 2004
AlwI	GGATC	6 cut(s)	314, 1446, 1470, 2059, 2164, 2264
AlwNI	CAGNNNCTG	2 cut(s)	476, 690
Aor51HI	AGCGCT	1 cut(s)	67
AoxI	GGCC	8 cut(s)	45, 75, 126, 510, 709, 960, 1021, 1934
ApaI	GGGCCC	1 cut(s)	130
ApeKI	GCWGC	4 cut(s)	659, 662, 684, 812
ApoI	RAATTY	4 cut(s)	429, 1693, 1773, 2141
Asp700I	GAANNNNTTC	1 cut(s)	1180
Asp718I	GGTACC	1 cut(s)	575
AspLEI	GCGC	5 cut(s)	16, 68, 242, 1837, 2456
AspS9I	GGNCC	4 cut(s)	76, 126, 127, 960
AsuC2I	CCSGG	1 cut(s)	79
AsuHPI	GGTGA	5 cut(s)	302, 731, 965, 1598, 2327
AsuII	TTCGAA	1 cut(s)	1174
AxyI	CCTNAGG	2 cut(s)	39, 1403
BaeGI	GKGCMC	1 cut(s)	130
BalI	TGGCCA	1 cut(s)	1023
BanI	GGYRCC	4 cut(s)	13, 81, 239, 575
BanII	GRGCYC	1 cut(s)	130
BauI	CACGAG	1 cut(s)	1866
BbsI	GAAGAC	1 cut(s)	1245
BbvI	GCAGC	4 cut(s)	649, 671, 696, 799
BccI	CCATC	8 cut(s)	829, 883, 950, 1237, 1321, 1919, 2227, 2242
BceAI	ACGGC	3 cut(s)	32, 253, 497
BcgI	CGANNNNNNTGC	2 cut(s)	804, 838
BciT130I	CCWGG	3 cut(s)	746, 2037, 2309
BclI	TGATCA	2 cut(s)	1462, 1603
BcnI	CCSGG	1 cut(s)	79
BcoDI	GTCTC	7 cut(s)	207, 547, 1191, 1426, 1736, 1754, 2004
BfaI	CTAG	4 cut(s)	713, 851, 1125, 1526
BfmI	CTRYAG	2 cut(s)	492, 933
BfoI	RGCGCY	3 cut(s)	17, 69, 243
BfuAI	ACCTGC	1 cut(s)	454
BglII	AGATCT	1 cut(s)	1634
BlpI	GCTNAGC	1 cut(s)	1521
BmcAI	AGTACT	1 cut(s)	1681
Bme1390I	CCNGG	4 cut(s)	79, 746, 2037, 2309
BmgT120I	GGNCC	4 cut(s)	76, 126, 127, 960
BmiI	GGNNCC	9 cut(s)	15, 83, 127, 128, 228, 241, 577, 828, 961
BmrFI	CCNGG	4 cut(s)	79, 746, 2037, 2309
BmrI	ACTGGG	1 cut(s)	1623
BmuI	ACTGGG	1 cut(s)	1623
BpiI	GAAGAC	1 cut(s)	1245
BpmI	CTGGAG	3 cut(s)	1660, 2167, 2340
Bpu10I	CCTNAGC	2 cut(s)	202, 2457
Bpu1102I	GCTNAGC	1 cut(s)	1521
Bpu14I	TTCGAA	1 cut(s)	1174
BpuEI	CTTGAG	1 cut(s)	547
BpuMI	CCSGG	1 cut(s)	79
BsaAI	YACGTR	2 cut(s)	263, 680
BsaBI	GATNNNNATC	2 cut(s)	858, 2114
BsaHI	GRCGYC	2 cut(s)	14, 240
BsaI	GGTCTC	3 cut(s)	547, 1426, 1736
BsaJI	CCNNGG	5 cut(s)	85, 513, 1735, 1998, 2035
BsaWI	WCCGGW	2 cut(s)	572, 1435
BsaXI	ACNNNNNCTCC	2 cut(s)	2140, 2170
Bsc4I	CCNNNNNNNGG	6 cut(s)	236, 1001, 1615, 2041, 2363, 2458
Bse118I	RCCGGY	1 cut(s)	10
Bse21I	CCTNAGG	2 cut(s)	39, 1403
Bse3DI	GCAATG	2 cut(s)	421, 2437
Bse8I	GATNNNNATC	2 cut(s)	858, 2114
BseBI	CCWGG	3 cut(s)	746, 2037, 2309
BseDI	CCNNGG	5 cut(s)	85, 513, 1735, 1998, 2035
BseJI	GATNNNNATC	2 cut(s)	858, 2114
BseLI	CCNNNNNNNGG	6 cut(s)	236, 1001, 1615, 2041, 2363, 2458
BseMI	GCAATG	2 cut(s)	421, 2437
BseMII	CTCAG	7 cut(s)	30, 535, 1044, 1357, 1496, 1512, 2220
BseRI	GAGGAG	5 cut(s)	233, 236, 239, 517, 1544
BseSI	GKGCMC	1 cut(s)	130
BseX3I	CGGCCG	1 cut(s)	45
BseXI	GCAGC	4 cut(s)	649, 671, 696, 799
BsgI	GTGCAG	2 cut(s)	462, 1845
Bsh1236I	CGCG	2 cut(s)	194, 2454
Bsh1285I	CGRYCG	1 cut(s)	48
BshFI	GGCC	8 cut(s)	47, 77, 128, 512, 711, 962, 1023, 1936
BshNI	GGYRCC	4 cut(s)	13, 81, 239, 575
BsiEI	CGRYCG	1 cut(s)	48
BsiSI	CCGG	4 cut(s)	11, 79, 573, 1436
BslFI	GGGAC	3 cut(s)	6, 1678, 2018
BslI	CCNNNNNNNGG	6 cut(s)	236, 1001, 1615, 2041, 2363, 2458
BsmAI	GTCTC	7 cut(s)	207, 547, 1191, 1426, 1736, 1754, 2004
BsmBI	CGTCTC	2 cut(s)	207, 1191
BsmFI	GGGAC	3 cut(s)	6, 1678, 2018
BsmI	GAATGC	1 cut(s)	1516
BsnI	GGCC	8 cut(s)	47, 77, 128, 512, 711, 962, 1023, 1936
Bso31I	GGTCTC	3 cut(s)	547, 1426, 1736
Bsp119I	TTCGAA	1 cut(s)	1174
Bsp120I	GGGCCC	1 cut(s)	126
Bsp1286I	GDGCHC	1 cut(s)	130
Bsp1407I	TGTACA	1 cut(s)	1389
Bsp1720I	GCTNAGC	1 cut(s)	1521
Bsp19I	CCATGG	1 cut(s)	1998
BspANI	GGCC	8 cut(s)	47, 77, 128, 512, 711, 962, 1023, 1936
BspCNI	CTCAG	7 cut(s)	31, 534, 1045, 1356, 1495, 1513, 2219
BspFNI	CGCG	2 cut(s)	194, 2454
BspHI	TCATGA	1 cut(s)	337
BspLI	GGNNCC	9 cut(s)	15, 83, 127, 128, 228, 241, 577, 828, 961
BspMAI	CTGCAG	1 cut(s)	496
BspMI	ACCTGC	1 cut(s)	454
BspPI	GGATC	6 cut(s)	314, 1446, 1470, 2059, 2164, 2264
BspT104I	TTCGAA	1 cut(s)	1174
BspT107I	GGYRCC	4 cut(s)	13, 81, 239, 575
BspTNI	GGTCTC	3 cut(s)	547, 1426, 1736
BsrBI	CCGCTC	1 cut(s)	140
BsrDI	GCAATG	2 cut(s)	421, 2437
BsrFI	RCCGGY	1 cut(s)	10
BsrGI	TGTACA	1 cut(s)	1389
BssAI	RCCGGY	1 cut(s)	10
BssECI	CCNNGG	5 cut(s)	85, 513, 1735, 1998, 2035
BssNAI	GTATAC	2 cut(s)	926, 1625
BssNI	GRCGYC	2 cut(s)	14, 240
BssSI	CACGAG	1 cut(s)	1866
BssT1I	CCWWGG	1 cut(s)	1998
Bst1107I	GTATAC	2 cut(s)	926, 1625
Bst2BI	CACGAG	1 cut(s)	1866
Bst2UI	CCWGG	3 cut(s)	746, 2037, 2309
Bst4CI	ACNGT	5 cut(s)	268, 641, 937, 1301, 2022
Bst6I	CTCTTC	4 cut(s)	149, 970, 1362, 1779
BstACI	GRCGYC	2 cut(s)	14, 240
BstAPI	GCANNNNNTGC	3 cut(s)	420, 446, 656
BstAUI	TGTACA	1 cut(s)	1389
BstBAI	YACGTR	2 cut(s)	263, 680
BstBI	TTCGAA	1 cut(s)	1174
BstC8I	GCNNGC	6 cut(s)	12, 421, 1009, 1598, 1820, 2402
BstDSI	CCRYGG	3 cut(s)	513, 1735, 1998
BstFNI	CGCG	2 cut(s)	194, 2454
BstH2I	RGCGCY	3 cut(s)	17, 69, 243
BstHHI	GCGC	5 cut(s)	16, 68, 242, 1837, 2456
BstMAI	GTCTC	7 cut(s)	207, 547, 1191, 1426, 1736, 1754, 2004
BstMCI	CGRYCG	1 cut(s)	48
BstNI	CCWGG	3 cut(s)	746, 2037, 2309
BstNSI	RCATGY	5 cut(s)	423, 737, 1042, 2060, 2404
BstSCI	CCNGG	4 cut(s)	77, 744, 2035, 2307
BstSFI	CTRYAG	2 cut(s)	492, 933
BstSLI	GKGCMC	1 cut(s)	130
BstSNI	TACGTA	1 cut(s)	680
BstUI	CGCG	2 cut(s)	194, 2454
BstV1I	GCAGC	4 cut(s)	649, 671, 696, 799
BstV2I	GAAGAC	1 cut(s)	1245
BstX2I	RGATCY	4 cut(s)	1475, 1634, 2064, 2169
BstXI	CCANNNNNNTGG	1 cut(s)	1031
BstYI	RGATCY	4 cut(s)	1475, 1634, 2064, 2169
BstZ17I	GTATAC	2 cut(s)	926, 1625
BstZI	CGGCCG	1 cut(s)	45
Bsu36I	CCTNAGG	2 cut(s)	39, 1403
BsuRI	GGCC	8 cut(s)	47, 77, 128, 512, 711, 962, 1023, 1936
BtgI	CCRYGG	3 cut(s)	513, 1735, 1998
BtsIMutI	CAGTG	4 cut(s)	410, 624, 2027, 2285
BveI	ACCTGC	1 cut(s)	454
Cac8I	GCNNGC	6 cut(s)	12, 421, 1009, 1598, 1820, 2402
CaiI	CAGNNNCTG	2 cut(s)	476, 690
CciI	TCATGA	1 cut(s)	337
CfoI	GCGC	5 cut(s)	16, 68, 242, 1837, 2456
Cfr10I	RCCGGY	1 cut(s)	10
Cfr13I	GGNCC	4 cut(s)	76, 126, 127, 960
Csp6I	GTAC	9 cut(s)	174, 264, 576, 612, 677, 1390, 1680, 1992, 2120
CspCI	CAANNNNNGTGG	2 cut(s)	386, 421
CviQI	GTAC	9 cut(s)	174, 264, 576, 612, 677, 1390, 1680, 1992, 2120
DinI	GGCGCC	2 cut(s)	15, 241
DraIII	CACNNNGTG	1 cut(s)	2024
EaeI	YGGCCR	3 cut(s)	45, 510, 1021
EagI	CGGCCG	1 cut(s)	45
Eam1104I	CTCTTC	4 cut(s)	149, 970, 1362, 1779
EarI	CTCTTC	4 cut(s)	149, 970, 1362, 1779
EciI	GGCGGA	1 cut(s)	126
EclXI	CGGCCG	1 cut(s)	45
Eco105I	TACGTA	1 cut(s)	680
Eco130I	CCWWGG	1 cut(s)	1998
Eco24I	GRGCYC	1 cut(s)	130
Eco31I	GGTCTC	3 cut(s)	547, 1426, 1736
Eco32I	GATATC	1 cut(s)	343
Eco47III	AGCGCT	1 cut(s)	67
Eco52I	CGGCCG	1 cut(s)	45
Eco57I	CTGAAG	2 cut(s)	75, 90
Eco81I	CCTNAGG	2 cut(s)	39, 1403
EcoO109I	RGGNCCY	2 cut(s)	126, 960
EcoRI	GAATTC	1 cut(s)	429
EcoRII	CCWGG	3 cut(s)	744, 2035, 2307
EcoRV	GATATC	1 cut(s)	343
EcoT14I	CCWWGG	1 cut(s)	1998
EcoT22I	ATGCAT	4 cut(s)	421, 1040, 1585, 2402
EcoT38I	GRGCYC	1 cut(s)	130
EgeI	GGCGCC	2 cut(s)	15, 241
EheI	GGCGCC	2 cut(s)	15, 241
ErhI	CCWWGG	1 cut(s)	1998
Esp3I	CGTCTC	2 cut(s)	207, 1191
FaqI	GGGAC	3 cut(s)	6, 1678, 2018
FauNDI	CATATG	2 cut(s)	1026, 2388
FbaI	TGATCA	2 cut(s)	1462, 1603
FblI	GTMKAC	2 cut(s)	925, 1624
FriOI	GRGCYC	1 cut(s)	130
FspBI	CTAG	4 cut(s)	713, 851, 1125, 1526
GlaI	GCGC	5 cut(s)	15, 67, 241, 1836, 2455
GsuI	CTGGAG	3 cut(s)	1660, 2167, 2340
HaeII	RGCGCY	3 cut(s)	17, 69, 243
HaeIII	GGCC	8 cut(s)	47, 77, 128, 512, 711, 962, 1023, 1936
HapII	CCGG	4 cut(s)	11, 79, 573, 1436
HhaI	GCGC	5 cut(s)	16, 68, 242, 1837, 2456
Hin1I	GRCGYC	2 cut(s)	14, 240
Hin6I	GCGC	5 cut(s)	14, 66, 240, 1835, 2454
HinP1I	GCGC	5 cut(s)	14, 66, 240, 1835, 2454
HincII	GTYRAC	1 cut(s)	1139
HindII	GTYRAC	1 cut(s)	1139
HindIII	AAGCTT	1 cut(s)	1802
HinfI	GANTC	9 cut(s)	722, 782, 1176, 1266, 1959, 2045, 2051, 2210, 2294
HpaII	CCGG	4 cut(s)	11, 79, 573, 1436
HphI	GGTGA	5 cut(s)	302, 731, 965, 1598, 2327
Hpy166II	GTNNAC	4 cut(s)	926, 1139, 1625, 2159
Hpy8I	GTNNAC	4 cut(s)	926, 1139, 1625, 2159
Hpy99I	CGWCG	3 cut(s)	123, 239, 328
HpyAV	CCTTC	7 cut(s)	160, 542, 995, 1233, 1480, 2306, 2357
HpyCH4III	ACNGT	5 cut(s)	268, 641, 937, 1301, 2022
HpyCH4IV	ACGT	2 cut(s)	262, 679
HpySE526I	ACGT	2 cut(s)	262, 679
Hsp92I	GRCGYC	2 cut(s)	14, 240
HspAI	GCGC	5 cut(s)	14, 66, 240, 1835, 2454
KasI	GGCGCC	2 cut(s)	13, 239
KpnI	GGTACC	1 cut(s)	579
KroI	GCCGGC	1 cut(s)	10
KroNI	GCCGGC	1 cut(s)	12
Ksp22I	TGATCA	2 cut(s)	1462, 1603
Lsp1109I	GCAGC	4 cut(s)	649, 671, 696, 799
MaeI	CTAG	4 cut(s)	713, 851, 1125, 1526
MaeII	ACGT	2 cut(s)	262, 679
MaeIII	GTNAC	4 cut(s)	719, 1739, 2016, 2176
MbiI	CCGCTC	1 cut(s)	140
MfeI	CAATTG	4 cut(s)	654, 726, 879, 1971
MflI	RGATCY	4 cut(s)	1475, 1634, 2064, 2169
MhlI	GDGCHC	1 cut(s)	130
MlsI	TGGCCA	1 cut(s)	1023
MluNI	TGGCCA	1 cut(s)	1023
Mly113I	GGCGCC	2 cut(s)	14, 240
MlyI	GAGTC	1 cut(s)	716
MmeI	TCCRAC	2 cut(s)	587, 2252
Mox20I	TGGCCA	1 cut(s)	1023
Mph1103I	ATGCAT	4 cut(s)	421, 1040, 1585, 2402
MroNI	GCCGGC	1 cut(s)	10
MroXI	GAANNNNTTC	1 cut(s)	1180
MscI	TGGCCA	1 cut(s)	1023
MseI	TTAA	9 cut(s)	594, 1059, 1535, 1548, 1751, 1848, 2082, 2112, 2421
Msp20I	TGGCCA	1 cut(s)	1023
MspA1I	CMGCKG	3 cut(s)	32, 662, 812
MspI	CCGG	4 cut(s)	11, 79, 573, 1436
MspR9I	CCNGG	4 cut(s)	79, 746, 2037, 2309
MunI	CAATTG	4 cut(s)	654, 726, 879, 1971
Mva1269I	GAATGC	1 cut(s)	1516
MvaI	CCWGG	3 cut(s)	746, 2037, 2309
MvnI	CGCG	2 cut(s)	194, 2454
NaeI	GCCGGC	1 cut(s)	12
NarI	GGCGCC	2 cut(s)	14, 240
NciI	CCSGG	1 cut(s)	79
NcoI	CCATGG	1 cut(s)	1998
NdeI	CATATG	2 cut(s)	1026, 2388
NgoMIV	GCCGGC	1 cut(s)	10
NlaIV	GGNNCC	9 cut(s)	15, 83, 127, 128, 228, 241, 577, 828, 961
NmeAIII	GCCGAG	2 cut(s)	110, 1115
NmuCI	GTSAC	2 cut(s)	719, 2016
NsiI	ATGCAT	4 cut(s)	421, 1040, 1585, 2402
NspI	RCATGY	5 cut(s)	423, 737, 1042, 2060, 2404
NspV	TTCGAA	1 cut(s)	1174
PaeI	GCATGC	2 cut(s)	423, 2404
PagI	TCATGA	1 cut(s)	337
PctI	GAATGC	1 cut(s)	1516
PdiI	GCCGGC	1 cut(s)	12
PdmI	GAANNNNTTC	1 cut(s)	1180
PfeI	GAWTC	8 cut(s)	782, 1176, 1266, 1959, 2045, 2051, 2210, 2294
PflFI	GACNNNGTC	1 cut(s)	188
PfoI	TCCNGGA	2 cut(s)	744, 2307
PleI	GAGTC	1 cut(s)	716
PluTI	GGCGCC	2 cut(s)	17, 243
PpsI	GAGTC	1 cut(s)	716
Ppu21I	YACGTR	2 cut(s)	263, 680
PsiI	TTATAA	1 cut(s)	332
Psp6I	CCWGG	3 cut(s)	744, 2035, 2307
PspGI	CCWGG	3 cut(s)	744, 2035, 2307
PspN4I	GGNNCC	9 cut(s)	15, 83, 127, 128, 228, 241, 577, 828, 961
PspOMI	GGGCCC	1 cut(s)	126
PspPI	GGNCC	4 cut(s)	76, 126, 127, 960
PstI	CTGCAG	1 cut(s)	496
PstNI	CAGNNNCTG	2 cut(s)	476, 690
PsuI	RGATCY	4 cut(s)	1475, 1634, 2064, 2169
PsyI	GACNNNGTC	1 cut(s)	188
PvuII	CAGCTG	2 cut(s)	662, 812
RsaI	GTAC	9 cut(s)	175, 265, 577, 613, 678, 1391, 1681, 1993, 2121
RsaNI	GTAC	9 cut(s)	174, 264, 576, 612, 677, 1390, 1680, 1992, 2120
SaqAI	TTAA	9 cut(s)	594, 1059, 1535, 1548, 1751, 1848, 2082, 2112, 2421
Sau96I	GGNCC	4 cut(s)	76, 126, 127, 960
ScaI	AGTACT	1 cut(s)	1681
SchI	GAGTC	1 cut(s)	716
ScrFI	CCNGG	4 cut(s)	79, 746, 2037, 2309
SduI	GDGCHC	1 cut(s)	130
SfcI	CTRYAG	2 cut(s)	492, 933
SfoI	GGCGCC	2 cut(s)	15, 241
SfuI	TTCGAA	1 cut(s)	1174
SmlI	CTYRAG	1 cut(s)	562
SmoI	CTYRAG	1 cut(s)	562
SnaBI	TACGTA	1 cut(s)	680
SphI	GCATGC	2 cut(s)	423, 2404
SspDI	GGCGCC	2 cut(s)	13, 239
SspI	AATATT	1 cut(s)	2097
SspMI	CTAG	4 cut(s)	713, 851, 1125, 1526
StyD4I	CCNGG	4 cut(s)	77, 744, 2035, 2307
StyI	CCWWGG	1 cut(s)	1998
TaaI	ACNGT	5 cut(s)	268, 641, 937, 1301, 2022
TaiI	ACGT	2 cut(s)	265, 682
TaqI	TCGA	7 cut(s)	1174, 1287, 1293, 1653, 1988, 2284, 2463
TatI	WGTACW	3 cut(s)	611, 1389, 1679
TauI	GCSGC	9 cut(s)	32, 47, 77, 102, 105, 108, 111, 1939, 2454
TfiI	GAWTC	8 cut(s)	782, 1176, 1266, 1959, 2045, 2051, 2210, 2294
Tru1I	TTAA	9 cut(s)	594, 1059, 1535, 1548, 1751, 1848, 2082, 2112, 2421
Tru9I	TTAA	9 cut(s)	594, 1059, 1535, 1548, 1751, 1848, 2082, 2112, 2421
TscAI	CASTG	4 cut(s)	410, 631, 2027, 2285
TseFI	GTSAC	2 cut(s)	719, 2016
TseI	GCWGC	4 cut(s)	659, 662, 684, 812
Tsp45I	GTSAC	2 cut(s)	719, 2016
TspGWI	ACGGA	2 cut(s)	312, 1724
TspRI	CASTG	4 cut(s)	410, 631, 2027, 2285
Tth111I	GACNNNGTC	1 cut(s)	188
XapI	RAATTY	4 cut(s)	429, 1693, 1773, 2141
XbaI	TCTAGA	1 cut(s)	850
XceI	RCATGY	5 cut(s)	423, 737, 1042, 2060, 2404
XmiI	GTMKAC	2 cut(s)	925, 1624
XmnI	GAANNNNTTC	1 cut(s)	1180
XspI	CTAG	4 cut(s)	713, 851, 1125, 1526
ZrmI	AGTACT	1 cut(s)	1681
Zsp2I	ATGCAT	4 cut(s)	421, 1040, 1585, 2402

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.