Rw4G012080

Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0015393
Physical Location & Seq
Reverse (-)
28399402 .. 28401089
1688 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G012080.1

Sequence Viewer

Length: 996 bp
ATGACAAGAAACGAAAGAGTATTTGTGTTTCTTGTGGTCCTTCCACTTCTAGTGTCTCTGTGTTCGTCGGCGACCTTGGCTCCTTCTTCAAAACCACCACCACCACCACCAAAGCAAAACAAGTTGACCTGGCATTACTATAAAGTCACTAACACATGCCGCGATGCAGAAGCTTTCATCCAGCACCAGGTCAAGAAGTTTTGGGACCAGGATAAAAGCATCACCGCTAAGCTTCTCCGGTTGCTCTACTCTGACTGCTTCGTAACTGGTTGTGATGCATCAGTACTGCTGGATGGTCCAAACTCTGAGAAAACAGCACCACAGAATTGGGGACTGGGAGGTTTCTATTTCATCGACAACGTCAAAAAAGTTCTTGAACATTACTGCCCCGGTGCCGTCTCGTGTGCTGATATTCTCAACCTTGCCACCAGGGATGCTGTTCATTTGGCTGGTGCACCTTCTTATCCTGTTCTTACAGGCCGAAGGGACGGCCTAACATCAAGCAGATCATCAGTCGACCTTCCCTCACCCTCCATTTCATGGCAGGCTTCACTTGACTACTTCAAATCCAGAGGCTTGAATGTATTGGATATGACTACTCTACTAGGTTCACATTCAATGGGTAAAACACACTGTCGCTTCATTTTGGATCGGCTCTACAATTTTAATGGCACAAACAAGCCAGACCCAAGCATGGAACCCTCATTCTTATCCTCCATGAGAAAGTTATGCCCACCAAGAACTACAAAGGGACAGAGTGACCCGCTGGTGTACCTAAACCCCCAATCTGGGGCCAATTACAATTTTACACAATCCTACTACTCCAGAGTTTTATCCAAGAAAGCTGTGCTGGGAGATATCACCGATGAGTTTGCTGCTGGTTTTGAAGATTTTCGGAGGTCATATGCTTACTCAATGAGCAGAATGGGTGCTATCATGGTTTTGACAGGTAACCAGGGAGAGATACGCCGGAATTGCCGAGTTCCAAACCACTAA

Protein Analysis

331

Amino Acids

36.9

Weight (kDa)

9.21

Isoelectric Point (pI)

45.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 61 - 284 2.8e-65 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015393)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43480 AT5G24070
fragaria_vesca FvH4_3g26850
malus_domestica MD04G1046400.v1.1
prunus_persica Prupe.1G199900_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0407891
rosa_laevigata RLG00000008691
rosa_multiflora Rmu_co8108400.1_g000001 Rmu_sc0029075.1_g000001
rosa_roxburghii Rroxscaffold_5G00352430
rosa_rugosa Rorug04G0082000
rosa_samantha Rh4AG147100 Rh4BG144600 Rh4CG153900 Rh4DG141100
rosa_wichuraiana Rw4G012080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 392
AccB7I CCANNNNNTGG 1 cut(s) 540
AccI GTMKAC 1 cut(s) 516
AccII CGCG 1 cut(s) 162
AciI CCGC 3 cut(s) 160, 225, 764
AclWI GGATC 1 cut(s) 657
AfaI GTAC 2 cut(s) 285, 773
AfiI CCNNNNNNNGG 6 cut(s) 187, 540, 694, 788, 789, 790
AgsI TTSAA 6 cut(s) 90, 377, 565, 580, 618, 887
AjnI CCWGG 5 cut(s) 128, 186, 207, 428, 954
AluBI AGCT 3 cut(s) 173, 232, 845
AluI AGCT 3 cut(s) 173, 232, 845
Alw21I GWGCWC 1 cut(s) 457
Alw26I GTCTC 2 cut(s) 60, 403
Alw44I GTGCAC 1 cut(s) 453
AlwI GGATC 1 cut(s) 657
AoxI GGCC 3 cut(s) 478, 490, 792
ApaLI GTGCAC 1 cut(s) 453
ApeKI GCWGC 1 cut(s) 875
Asp700I GAANNNNTTC 1 cut(s) 891
AspS9I GGNCC 4 cut(s) 37, 205, 296, 792
AsuC2I CCSGG 1 cut(s) 390
AsuHPI GGTGA 3 cut(s) 214, 519, 853
AvaII GGWCC 3 cut(s) 37, 205, 296
BaeGI GKGCMC 1 cut(s) 457
BanI GGYRCC 1 cut(s) 392
BauI CACGAG 1 cut(s) 400
Bbv12I GWGCWC 1 cut(s) 457
BbvI GCAGC 1 cut(s) 862
BccI CCATC 1 cut(s) 287
BceAI ACGGC 2 cut(s) 380, 505
BcgI CGANNNNNNTGC 2 cut(s) 854, 888
BciT130I CCWGG 5 cut(s) 130, 188, 209, 430, 956
BcnI CCSGG 1 cut(s) 390
BcoDI GTCTC 2 cut(s) 60, 403
BfaI CTAG 2 cut(s) 50, 605
BisI GCNGC 2 cut(s) 160, 876
BlpI GCTNAGC 1 cut(s) 228
BlsI GCNGC 2 cut(s) 161, 877
BmcAI AGTACT 1 cut(s) 285
Bme1390I CCNGG 6 cut(s) 130, 188, 209, 390, 430, 956
Bme18I GGWCC 3 cut(s) 37, 205, 296
BmgT120I GGNCC 4 cut(s) 37, 205, 296, 792
BmiI GGNNCC 5 cut(s) 81, 206, 394, 699, 793
BmrFI CCNGG 6 cut(s) 130, 188, 209, 390, 430, 956
BmrI ACTGGG 1 cut(s) 344
BmsI GCATC 5 cut(s) 154, 228, 265, 287, 424
BmuI ACTGGG 1 cut(s) 344
BpmI CTGGAG 1 cut(s) 808
Bpu1102I GCTNAGC 1 cut(s) 228
BpuMI CCSGG 1 cut(s) 390
BsaJI CCNNGG 4 cut(s) 75, 388, 429, 955
BsaWI WCCGGW 1 cut(s) 237
BsaXI ACNNNNNCTCC 2 cut(s) 64, 94
Bsc4I CCNNNNNNNGG 6 cut(s) 187, 540, 694, 788, 789, 790
Bse1I ACTGG 2 cut(s) 271, 339
BseBI CCWGG 5 cut(s) 130, 188, 209, 430, 956
BseDI CCNNGG 4 cut(s) 75, 388, 429, 955
BseGI GGATG 3 cut(s) 177, 298, 439
BseLI CCNNNNNNNGG 6 cut(s) 187, 540, 694, 788, 789, 790
BseMII CTCAG 1 cut(s) 297
BseNI ACTGG 2 cut(s) 271, 339
BseSI GKGCMC 1 cut(s) 457
BseXI GCAGC 1 cut(s) 862
BseYI CCCAGC 1 cut(s) 850
Bsh1236I CGCG 1 cut(s) 162
BshFI GGCC 3 cut(s) 480, 492, 794
BshNI GGYRCC 1 cut(s) 392
BsiHKAI GWGCWC 1 cut(s) 457
BsiSI CCGG 3 cut(s) 238, 390, 970
BslFI GGGAC 4 cut(s) 218, 345, 500, 765
BslI CCNNNNNNNGG 6 cut(s) 187, 540, 694, 788, 789, 790
BsmAI GTCTC 2 cut(s) 60, 403
BsmBI CGTCTC 1 cut(s) 403
BsmFI GGGAC 4 cut(s) 218, 345, 500, 765
BsnI GGCC 3 cut(s) 480, 492, 794
Bsp1286I GDGCHC 1 cut(s) 457
Bsp143I GATC 2 cut(s) 506, 649
Bsp1720I GCTNAGC 1 cut(s) 228
BspACI CCGC 3 cut(s) 160, 225, 764
BspANI GGCC 3 cut(s) 480, 492, 794
BspCNI CTCAG 1 cut(s) 298
BspFNI CGCG 1 cut(s) 162
BspLI GGNNCC 5 cut(s) 81, 206, 394, 699, 793
BspPI GGATC 1 cut(s) 657
BspT107I GGYRCC 1 cut(s) 392
BsrI ACTGG 2 cut(s) 271, 339
BssECI CCNNGG 4 cut(s) 75, 388, 429, 955
BssMI GATC 2 cut(s) 506, 649
BssSI CACGAG 1 cut(s) 400
BssT1I CCWWGG 1 cut(s) 75
Bst2BI CACGAG 1 cut(s) 400
Bst2UI CCWGG 5 cut(s) 130, 188, 209, 430, 956
Bst4CI ACNGT 1 cut(s) 635
BstC8I GCNNGC 1 cut(s) 546
BstDEI CTNAG 2 cut(s) 228, 306
BstEII GGTNACC 1 cut(s) 950
BstF5I GGATG 3 cut(s) 177, 298, 439
BstFNI CGCG 1 cut(s) 162
BstKTI GATC 2 cut(s) 509, 652
BstMAI GTCTC 2 cut(s) 60, 403
BstMBI GATC 2 cut(s) 506, 649
BstMWI GCNNNNNNNGC 2 cut(s) 77, 975
BstNI CCWGG 5 cut(s) 130, 188, 209, 430, 956
BstNSI RCATGY 1 cut(s) 159
BstPI GGTNACC 1 cut(s) 950
BstSCI CCNGG 6 cut(s) 128, 186, 207, 388, 428, 954
BstSLI GKGCMC 1 cut(s) 457
BstUI CGCG 1 cut(s) 162
BstV1I GCAGC 1 cut(s) 862
BstXI CCANNNNNNTGG 1 cut(s) 327
BsuRI GGCC 3 cut(s) 480, 492, 794
BtgZI GCGATG 1 cut(s) 177
BtsCI GGATG 3 cut(s) 177, 298, 439
BtsIMutI CAGTG 1 cut(s) 631
Cac8I GCNNGC 1 cut(s) 546
Cfr13I GGNCC 4 cut(s) 37, 205, 296, 792
CsiI ACCWGGT 1 cut(s) 186
Csp6I GTAC 2 cut(s) 284, 772
CviAII CATG 5 cut(s) 156, 540, 694, 718, 937
CviQI GTAC 2 cut(s) 284, 772
DdeI CTNAG 2 cut(s) 228, 306
DpnI GATC 2 cut(s) 508, 651
DpnII GATC 2 cut(s) 506, 649
Eco130I CCWWGG 1 cut(s) 75
Eco32I GATATC 1 cut(s) 859
Eco47I GGWCC 3 cut(s) 37, 205, 296
Eco91I GGTNACC 1 cut(s) 950
EcoO65I GGTNACC 1 cut(s) 950
EcoRII CCWGG 5 cut(s) 128, 186, 207, 428, 954
EcoRV GATATC 1 cut(s) 859
EcoT14I CCWWGG 1 cut(s) 75
EcoT22I ATGCAT 1 cut(s) 280
ErhI CCWWGG 1 cut(s) 75
Esp3I CGTCTC 1 cut(s) 403
FaeI CATG 5 cut(s) 159, 543, 697, 721, 940
FaqI GGGAC 4 cut(s) 218, 345, 500, 765
FatI CATG 5 cut(s) 155, 539, 693, 717, 936
FauI CCCGC 1 cut(s) 771
FauNDI CATATG 1 cut(s) 904
FblI GTMKAC 1 cut(s) 516
Fnu4HI GCNGC 2 cut(s) 160, 876
FokI GGATG 3 cut(s) 164, 305, 446
Fsp4HI GCNGC 2 cut(s) 160, 876
FspBI CTAG 2 cut(s) 50, 605
GluI GCNGC 2 cut(s) 160, 876
GsaI CCCAGC 1 cut(s) 854
GsuI CTGGAG 1 cut(s) 808
HaeIII GGCC 3 cut(s) 480, 492, 794
HapII CCGG 3 cut(s) 238, 390, 970
Hin1II CATG 5 cut(s) 159, 543, 697, 721, 940
HincII GTYRAC 2 cut(s) 126, 517
HindII GTYRAC 2 cut(s) 126, 517
HindIII AAGCTT 2 cut(s) 171, 230
HpaII CCGG 3 cut(s) 238, 390, 970
HphI GGTGA 3 cut(s) 214, 519, 853
Hpy166II GTNNAC 5 cut(s) 126, 455, 517, 611, 772
Hpy188I TCNGA 3 cut(s) 253, 307, 897
Hpy188III TCNNGA 4 cut(s) 193, 374, 570, 825
Hpy8I GTNNAC 5 cut(s) 126, 455, 517, 611, 772
Hpy99I CGWCG 1 cut(s) 70
HpyAV CCTTC 5 cut(s) 50, 93, 468, 477, 530
HpyCH4III ACNGT 1 cut(s) 635
HpyCH4IV ACGT 1 cut(s) 360
HpyCH4V TGCA 3 cut(s) 167, 278, 455
HpyF10VI GCNNNNNNNGC 2 cut(s) 77, 975
HpyF3I CTNAG 2 cut(s) 228, 306
HpySE526I ACGT 1 cut(s) 360
Hsp92II CATG 5 cut(s) 159, 543, 697, 721, 940
Kzo9I GATC 2 cut(s) 506, 649
LmnI GCTCC 1 cut(s) 85
Lsp1109I GCAGC 1 cut(s) 862
LweI GCATC 5 cut(s) 154, 228, 265, 287, 424
MabI ACCWGGT 1 cut(s) 186
MaeI CTAG 2 cut(s) 50, 605
MaeII ACGT 1 cut(s) 360
MaeIII GTNAC 4 cut(s) 145, 262, 758, 950
MalI GATC 2 cut(s) 508, 651
MboI GATC 2 cut(s) 506, 649
MboII GAAGA 2 cut(s) 78, 899
MhlI GDGCHC 1 cut(s) 457
MluCI AATT 5 cut(s) 325, 661, 796, 802, 973
MnlI CCTC 7 cut(s) 332, 535, 541, 566, 712, 724, 891
Mph1103I ATGCAT 1 cut(s) 280
MroXI GAANNNNTTC 1 cut(s) 891
MseI TTAA 1 cut(s) 666
MspA1I CMGCKG 1 cut(s) 766
MspI CCGG 3 cut(s) 238, 390, 970
MspR9I CCNGG 6 cut(s) 130, 188, 209, 390, 430, 956
MvaI CCWGG 5 cut(s) 130, 188, 209, 430, 956
MvnI CGCG 1 cut(s) 162
MwoI GCNNNNNNNGC 2 cut(s) 77, 975
NciI CCSGG 1 cut(s) 390
NdeI CATATG 1 cut(s) 904
NdeII GATC 2 cut(s) 506, 649
NlaIII CATG 5 cut(s) 159, 543, 697, 721, 940
NlaIV GGNNCC 5 cut(s) 81, 206, 394, 699, 793
NmuCI GTSAC 2 cut(s) 145, 758
NsiI ATGCAT 1 cut(s) 280
NspI RCATGY 1 cut(s) 159
PdmI GAANNNNTTC 1 cut(s) 891
PflFI GACNNNGTC 1 cut(s) 359
PflMI CCANNNNNTGG 1 cut(s) 540
PkrI GCNGC 2 cut(s) 161, 877
Psp6I CCWGG 5 cut(s) 128, 186, 207, 428, 954
PspEI GGTNACC 1 cut(s) 950
PspFI CCCAGC 1 cut(s) 850
PspGI CCWGG 5 cut(s) 128, 186, 207, 428, 954
PspN4I GGNNCC 5 cut(s) 81, 206, 394, 699, 793
PspPI GGNCC 4 cut(s) 37, 205, 296, 792
PsyI GACNNNGTC 1 cut(s) 359
RsaI GTAC 2 cut(s) 285, 773
RsaNI GTAC 2 cut(s) 284, 772
SalI GTCGAC 1 cut(s) 515
SaqAI TTAA 1 cut(s) 666
SatI GCNGC 2 cut(s) 160, 876
Sau3AI GATC 2 cut(s) 506, 649
Sau96I GGNCC 4 cut(s) 37, 205, 296, 792
ScaI AGTACT 1 cut(s) 285
ScrFI CCNGG 6 cut(s) 130, 188, 209, 390, 430, 956
SduI GDGCHC 1 cut(s) 457
SexAI ACCWGGT 1 cut(s) 186
SfaNI GCATC 5 cut(s) 154, 228, 265, 287, 424
SinI GGWCC 3 cut(s) 37, 205, 296
Sse9I AATT 5 cut(s) 325, 661, 796, 802, 973
SsiI CCGC 3 cut(s) 160, 225, 764
SspMI CTAG 2 cut(s) 50, 605
StyD4I CCNGG 6 cut(s) 128, 186, 207, 388, 428, 954
StyI CCWWGG 1 cut(s) 75
TaaI ACNGT 1 cut(s) 635
TaiI ACGT 1 cut(s) 363
TaqI TCGA 2 cut(s) 354, 516
TasI AATT 5 cut(s) 325, 661, 796, 802, 973
TatI WGTACW 1 cut(s) 283
TauI GCSGC 1 cut(s) 162
Tru1I TTAA 1 cut(s) 666
Tru9I TTAA 1 cut(s) 666
TscAI CASTG 1 cut(s) 638
TseFI GTSAC 2 cut(s) 145, 758
TseI GCWGC 1 cut(s) 875
Tsp45I GTSAC 2 cut(s) 145, 758
TspDTI ATGAA 5 cut(s) 166, 340, 431, 528, 631
TspRI CASTG 1 cut(s) 638
Tth111I GACNNNGTC 1 cut(s) 359
Van91I CCANNNNNTGG 1 cut(s) 540
VneI GTGCAC 1 cut(s) 453
VpaK11BI GGWCC 3 cut(s) 37, 205, 296
XceI RCATGY 1 cut(s) 159
XmiI GTMKAC 1 cut(s) 516
XmnI GAANNNNTTC 1 cut(s) 891
XspI CTAG 2 cut(s) 50, 605
ZrmI AGTACT 1 cut(s) 285
Zsp2I ATGCAT 1 cut(s) 280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.