Rorug04G0143500

Protein TIME FOR

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0007100
Physical Location & Seq
Forward (+)
23917188 .. 23918407
1220 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0143500.1

Sequence Viewer

Length: 675 bp
ATGGCTTTCCAAGTTGCAGTCTTCACCATCCTAGGGCTAGCATTTGTAGCCCAAATTGCTGTTGCTCAAAGCGTGGCAGATATCGTTACAGTGGACTTCTTCAACGGGATTATTAACCAAGCTGCCGCTGATTGTGCCGGCAAGAGCTTCTACACCAGAGATGCTTTTCTCAATGCCGTAGGCTCTTACCCCAATTTCGGTACCTCCGGTTCCGCTGATGAGAATAAAAGGGAAATTGCAGCTTTCTTTGCTCACGTCACTCATGAGACTGGCCATTTTTGCTACATTGAAGAGATAAATAAGAGCACCTACTGCGACGCGAGCAGAACAGACTACCCATGCAACGCCAACAAGCAGTACTACGGGCGTGGACCACTCCAGCTCACCTGGAACTACAATTACGGAGCTGCCGGAAATGCCATTGGCTTTGACGGCTTGAACTCCCCCGAAACGGTGGCTCAAGACCCCGTGATAGCATTCAAGACAGGCTTGTGGTATTGGATGACCAATGTTCACTCCGTTATAGGCCAAGGATTCGGAGCCACAACTCGAGCCATCAACGGTGCTGTGGAATGTGATGGCAAATCCCCAGCCACTGTTCAAGCTCGCGCCGACTATTACACTAACTACTGCACCCAATTTAATGTTGCTCCCGGTGACAATCTCTTGTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

224

Amino Acids

24.23

Weight (kDa)

4.91

Isoelectric Point (pI)

28.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_19 PF00182 28 - 171 2.3e-42 Chitinase class I
Glyco_hydro_19 PF00182 178 - 224 1.3e-07 Chitinase class I
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 200
AccB1I GGYRCC 1 cut(s) 200
AccII CGCG 2 cut(s) 320, 609
AciI CCGC 2 cut(s) 126, 213
AcoI YGGCCR 1 cut(s) 271
AfaI GTAC 2 cut(s) 202, 359
AfiI CCNNNNNNNGG 3 cut(s) 33, 197, 451
AgsI TTSAA 5 cut(s) 103, 290, 439, 481, 602
AjiI CACGTC 1 cut(s) 256
AjnI CCWGG 1 cut(s) 386
AluBI AGCT 6 cut(s) 122, 147, 242, 382, 407, 605
AluI AGCT 6 cut(s) 122, 147, 242, 382, 407, 605
Alw21I GWGCWC 1 cut(s) 308
Alw26I GTCTC 1 cut(s) 260
AlwNI CAGNNNCTG 1 cut(s) 596
Ama87I CYCGRG 1 cut(s) 549
AoxI GGCC 2 cut(s) 271, 526
ApeKI GCWGC 3 cut(s) 122, 239, 407
Asp718I GGTACC 1 cut(s) 200
AspA2I CCTAGG 1 cut(s) 31
AspLEI GCGC 1 cut(s) 611
AspS9I GGNCC 1 cut(s) 371
AsuC2I CCSGG 1 cut(s) 654
AsuHPI GGTGA 3 cut(s) 16, 376, 668
AsuNHI GCTAGC 1 cut(s) 37
AvaI CYCGRG 1 cut(s) 549
AvaII GGWCC 1 cut(s) 371
AvrII CCTAGG 1 cut(s) 31
BalI TGGCCA 1 cut(s) 273
BanI GGYRCC 1 cut(s) 200
BbsI GAAGAC 1 cut(s) 13
Bbv12I GWGCWC 1 cut(s) 308
BbvI GCAGC 3 cut(s) 109, 251, 394
BccI CCATC 3 cut(s) 35, 563, 572
BceAI ACGGC 2 cut(s) 161, 448
BciT130I CCWGG 1 cut(s) 388
BcnI CCSGG 1 cut(s) 654
BcoDI GTCTC 1 cut(s) 260
BfaI CTAG 3 cut(s) 32, 38, 673
BisI GCNGC 4 cut(s) 123, 126, 240, 408
BlnI CCTAGG 1 cut(s) 31
BlsI GCNGC 4 cut(s) 124, 127, 241, 409
BmcAI AGTACT 1 cut(s) 359
Bme1390I CCNGG 2 cut(s) 388, 654
Bme18I GGWCC 1 cut(s) 371
BmeT110I CYCGRG 1 cut(s) 549
BmgBI CACGTC 1 cut(s) 256
BmgT120I GGNCC 1 cut(s) 371
BmiI GGNNCC 3 cut(s) 202, 211, 541
BmrFI CCNGG 2 cut(s) 388, 654
BmsI GCATC 1 cut(s) 151
BmtI GCTAGC 1 cut(s) 41
BpiI GAAGAC 1 cut(s) 13
BpmI CTGGAG 1 cut(s) 362
BpuEI CTTGAG 1 cut(s) 444
BpuMI CCSGG 1 cut(s) 654
BsaJI CCNNGG 2 cut(s) 31, 529
BsaWI WCCGGW 1 cut(s) 206
Bsc4I CCNNNNNNNGG 3 cut(s) 33, 197, 451
Bse118I RCCGGY 1 cut(s) 137
Bse1I ACTGG 1 cut(s) 274
BseBI CCWGG 1 cut(s) 388
BseDI CCNNGG 2 cut(s) 31, 529
BseGI GGATG 2 cut(s) 27, 507
BseLI CCNNNNNNNGG 3 cut(s) 33, 197, 451
BseNI ACTGG 1 cut(s) 274
BseXI GCAGC 3 cut(s) 109, 251, 394
BseYI CCCAGC 1 cut(s) 589
BsgI GTGCAG 1 cut(s) 616
Bsh1236I CGCG 2 cut(s) 320, 609
BshFI GGCC 2 cut(s) 273, 528
BshNI GGYRCC 1 cut(s) 200
BsiHKAI GWGCWC 1 cut(s) 308
BsiHKCI CYCGRG 1 cut(s) 549
BsiSI CCGG 4 cut(s) 138, 207, 411, 654
BslI CCNNNNNNNGG 3 cut(s) 33, 197, 451
BsmAI GTCTC 1 cut(s) 260
BsmI GAATGC 1 cut(s) 476
BsnI GGCC 2 cut(s) 273, 528
BsoBI CYCGRG 1 cut(s) 549
Bsp1286I GDGCHC 1 cut(s) 308
BspACI CCGC 2 cut(s) 126, 213
BspANI GGCC 2 cut(s) 273, 528
BspFNI CGCG 2 cut(s) 320, 609
BspHI TCATGA 1 cut(s) 262
BspLI GGNNCC 3 cut(s) 202, 211, 541
BspOI GCTAGC 1 cut(s) 41
BspT107I GGYRCC 1 cut(s) 200
BsrFI RCCGGY 1 cut(s) 137
BsrI ACTGG 1 cut(s) 274
BssAI RCCGGY 1 cut(s) 137
BssECI CCNNGG 2 cut(s) 31, 529
BssT1I CCWWGG 2 cut(s) 31, 529
Bst2UI CCWGG 1 cut(s) 388
Bst4CI ACNGT 4 cut(s) 91, 454, 563, 598
Bst6I CTCTTC 1 cut(s) 285
BstAPI GCANNNNNTGC 1 cut(s) 312
BstC8I GCNNGC 4 cut(s) 39, 139, 322, 607
BstF5I GGATG 2 cut(s) 27, 507
BstFNI CGCG 2 cut(s) 320, 609
BstHHI GCGC 1 cut(s) 611
BstMAI GTCTC 1 cut(s) 260
BstMWI GCNNNNNNNGC 9 cut(s) 47, 56, 134, 248, 279, 312, 321, 416, 432
BstNI CCWGG 1 cut(s) 388
BstSCI CCNGG 2 cut(s) 386, 652
BstUI CGCG 2 cut(s) 320, 609
BstV1I GCAGC 3 cut(s) 109, 251, 394
BstV2I GAAGAC 1 cut(s) 13
BsuRI GGCC 2 cut(s) 273, 528
BtrI CACGTC 1 cut(s) 256
BtsCI GGATG 2 cut(s) 27, 507
BtsIMutI CAGTG 2 cut(s) 96, 594
Cac8I GCNNGC 4 cut(s) 39, 139, 322, 607
CaiI CAGNNNCTG 1 cut(s) 596
CciI TCATGA 1 cut(s) 262
CfoI GCGC 1 cut(s) 611
Cfr10I RCCGGY 1 cut(s) 137
Cfr13I GGNCC 1 cut(s) 371
CseI GACGC 1 cut(s) 326
Csp6I GTAC 2 cut(s) 201, 358
CviAII CATG 2 cut(s) 263, 339
CviQI GTAC 2 cut(s) 201, 358
EaeI YGGCCR 1 cut(s) 271
Eam1104I CTCTTC 1 cut(s) 285
EarI CTCTTC 1 cut(s) 285
Eco130I CCWWGG 2 cut(s) 31, 529
Eco32I GATATC 1 cut(s) 82
Eco47I GGWCC 1 cut(s) 371
Eco88I CYCGRG 1 cut(s) 549
EcoRII CCWGG 1 cut(s) 386
EcoRV GATATC 1 cut(s) 82
EcoT14I CCWWGG 2 cut(s) 31, 529
ErhI CCWWGG 2 cut(s) 31, 529
FaeI CATG 2 cut(s) 266, 342
FaiI YATR 3 cut(s) 264, 340, 524
FalI AAGNNNNNCTT 2 cut(s) 473, 505
FatI CATG 2 cut(s) 262, 338
Fnu4HI GCNGC 4 cut(s) 123, 126, 240, 408
FokI GGATG 2 cut(s) 14, 514
Fsp4HI GCNGC 4 cut(s) 123, 126, 240, 408
FspBI CTAG 3 cut(s) 32, 38, 673
GlaI GCGC 1 cut(s) 610
GluI GCNGC 4 cut(s) 123, 126, 240, 408
GsaI CCCAGC 1 cut(s) 593
GsuI CTGGAG 1 cut(s) 362
HaeIII GGCC 2 cut(s) 273, 528
HapII CCGG 4 cut(s) 138, 207, 411, 654
HgaI GACGC 1 cut(s) 326
HhaI GCGC 1 cut(s) 611
Hin1II CATG 2 cut(s) 266, 342
Hin6I GCGC 1 cut(s) 609
HinP1I GCGC 1 cut(s) 609
HinfI GANTC 1 cut(s) 534
HpaII CCGG 4 cut(s) 138, 207, 411, 654
HphI GGTGA 3 cut(s) 16, 376, 668
Hpy166II GTNNAC 3 cut(s) 94, 371, 514
Hpy188I TCNGA 1 cut(s) 539
Hpy188III TCNNGA 3 cut(s) 263, 461, 481
Hpy8I GTNNAC 3 cut(s) 94, 371, 514
Hpy99I CGWCG 1 cut(s) 320
HpyCH4III ACNGT 4 cut(s) 91, 454, 563, 598
HpyCH4IV ACGT 1 cut(s) 255
HpyCH4V TGCA 4 cut(s) 17, 239, 342, 633
HpyF10VI GCNNNNNNNGC 9 cut(s) 47, 56, 134, 248, 279, 312, 321, 416, 432
HpySE526I ACGT 1 cut(s) 255
Hsp92II CATG 2 cut(s) 266, 342
HspAI GCGC 1 cut(s) 609
KpnI GGTACC 1 cut(s) 204
KroI GCCGGC 1 cut(s) 137
KroNI GCCGGC 1 cut(s) 139
LmnI GCTCC 3 cut(s) 404, 539, 655
Lsp1109I GCAGC 3 cut(s) 109, 251, 394
LweI GCATC 1 cut(s) 151
MaeI CTAG 3 cut(s) 32, 38, 673
MaeII ACGT 1 cut(s) 255
MaeIII GTNAC 3 cut(s) 85, 256, 656
MboII GAAGA 3 cut(s) 13, 91, 302
MhlI GDGCHC 1 cut(s) 308
MlsI TGGCCA 1 cut(s) 273
MluCI AATT 5 cut(s) 54, 193, 234, 397, 638
MluNI TGGCCA 1 cut(s) 273
MnlI CCTC 1 cut(s) 214
Mox20I TGGCCA 1 cut(s) 273
MroNI GCCGGC 1 cut(s) 137
MscI TGGCCA 1 cut(s) 273
MseI TTAA 2 cut(s) 114, 642
Msp20I TGGCCA 1 cut(s) 273
MspA1I CMGCKG 2 cut(s) 128, 215
MspI CCGG 4 cut(s) 138, 207, 411, 654
MspR9I CCNGG 2 cut(s) 388, 654
Mva1269I GAATGC 1 cut(s) 476
MvaI CCWGG 1 cut(s) 388
MvnI CGCG 2 cut(s) 320, 609
MwoI GCNNNNNNNGC 9 cut(s) 47, 56, 134, 248, 279, 312, 321, 416, 432
NaeI GCCGGC 1 cut(s) 139
NciI CCSGG 1 cut(s) 654
NgoMIV GCCGGC 1 cut(s) 137
NheI GCTAGC 1 cut(s) 37
NlaIII CATG 2 cut(s) 266, 342
NlaIV GGNNCC 3 cut(s) 202, 211, 541
NmuCI GTSAC 2 cut(s) 256, 656
PaeR7I CTCGAG 1 cut(s) 549
PagI TCATGA 1 cut(s) 262
PctI GAATGC 1 cut(s) 476
PdiI GCCGGC 1 cut(s) 139
PfeI GAWTC 1 cut(s) 534
PkrI GCNGC 4 cut(s) 124, 127, 241, 409
Psp6I CCWGG 1 cut(s) 386
PspFI CCCAGC 1 cut(s) 589
PspGI CCWGG 1 cut(s) 386
PspN4I GGNNCC 3 cut(s) 202, 211, 541
PspPI GGNCC 1 cut(s) 371
PspXI VCTCGAGB 1 cut(s) 549
PsrI GAACNNNNNNTAC 4 cut(s) 193, 225, 383, 415
PstNI CAGNNNCTG 1 cut(s) 596
RsaI GTAC 2 cut(s) 202, 359
RsaNI GTAC 2 cut(s) 201, 358
SaqAI TTAA 2 cut(s) 114, 642
SatI GCNGC 4 cut(s) 123, 126, 240, 408
Sau96I GGNCC 1 cut(s) 371
ScaI AGTACT 1 cut(s) 359
ScrFI CCNGG 2 cut(s) 388, 654
SduI GDGCHC 1 cut(s) 308
SfaNI GCATC 1 cut(s) 151
Sfr274I CTCGAG 1 cut(s) 549
SinI GGWCC 1 cut(s) 371
SlaI CTCGAG 1 cut(s) 549
SmlI CTYRAG 2 cut(s) 459, 549
SmoI CTYRAG 2 cut(s) 459, 549
Sse9I AATT 5 cut(s) 54, 193, 234, 397, 638
SsiI CCGC 2 cut(s) 126, 213
SspMI CTAG 3 cut(s) 32, 38, 673
StyD4I CCNGG 2 cut(s) 386, 652
StyI CCWWGG 2 cut(s) 31, 529
TaaI ACNGT 4 cut(s) 91, 454, 563, 598
TaiI ACGT 1 cut(s) 258
TaqI TCGA 1 cut(s) 550
TasI AATT 5 cut(s) 54, 193, 234, 397, 638
TatI WGTACW 1 cut(s) 357
TauI GCSGC 1 cut(s) 128
TfiI GAWTC 1 cut(s) 534
Tru1I TTAA 2 cut(s) 114, 642
Tru9I TTAA 2 cut(s) 114, 642
TscAI CASTG 2 cut(s) 96, 601
TseFI GTSAC 2 cut(s) 256, 656
TseI GCWGC 3 cut(s) 122, 239, 407
Tsp45I GTSAC 2 cut(s) 256, 656
TspGWI ACGGA 2 cut(s) 417, 508
TspRI CASTG 2 cut(s) 96, 601
VpaK11BI GGWCC 1 cut(s) 371
XhoI CTCGAG 1 cut(s) 549
XmaJI CCTAGG 1 cut(s) 31
XspI CTAG 3 cut(s) 32, 38, 673
ZrmI AGTACT 1 cut(s) 359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.