Rorug04G0206900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0007338
Physical Location & Seq
Reverse (-)
36009452 .. 36012896
3445 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0206900.1

Sequence Viewer

Length: 2739 bp
ATGAAAAATATCCTCAGCTTTCTGCTATTGGTATTTGCTATCTCCGTTACATGCTCCCCTGCAATTGGCAGCAAATGTATCGGAGACCAGCAGGTATTATTACTCAATATCAAGAAAAGCCTTGTGTTCGATGCTTCTAGTTCGTCGAAGCTTGTTTCGTGGGATTCAAGTACAGATTGTTGTTCTTGGCTCGGCATTACATGCAGCAATGATGGACGTGTTTTCGGTGTTGACATCAGCGGAGAATCTATCTCGGGTGGAATTGACAATTCCAGCAGTCTCTTCCATCTTCAACACCTTCAAAGCCTCAACTTGGCTGACAACGCACTTGGTGATGGCCGCTCTCCAGTAATTCCATCGGCTATTGGACAGCTTAAGAATTTGAGGTATCTAAATTTATCTCACAATTTTTATCTGGGCCAAATTCCCATTCAGATTTCATACTTGACCAGGTTGGTGATTCTTGATATTTCTCACAACTTGTTAAAACTTGAGAGCCCAAATTTACACATTCTGGTTCGAAACCTCACAGAGCTCACAGAGTTATATCTTAATGAAGTCCAAATATCAGCACAAGGGCGTGATTGGGGCCAGGCCATATCATCTTCTCTTCCACATTTGAGGGTGCTAGACTTGTCCAATTGTGGTCTTTCAGGCCCTATCCATGAGTCCCTTGCTAAGCTTCAGTCTCTATCAGTGGTTCAATTGCAAGGGAATGACATCTCTGCACCAATTCCAGGCTTCTTTGCCCACTTTTCAAATTTGACTTTCTTGGGTCTCAGCAAATGCTTGTTGCAGGGAATATTTCCAAAAGAGATCTTTCACTTACCTACACTGCAAAGCATCGACCTCTCAGAGAATCCAGAACTCCATGGGTCCTTGCCAGAATTTCCGAAAAATGGATCTCTCCGGTCCTTGTTTCTTGGTGGATCAAAATTTTCAGGGTCGTTACCAAACTCGATTGGCAACCTCGCAATGTTGTCCACAGTAGATATCTCACTGTGCAATATCACTGGACCATTACCCAAGTCGATGGCAAACCTTACAGAATTGGTTAATCTGGACATGTCTGAAAATAACTTTGAAGGTTCAATTCCATCTTTTGATAGGGCCAAGAATCTGGAGAGCATAGACCTTTCTTTAAATGGTCTAACTGGCCGGTTCAGTGACAATATGCTCGAAGGCAATATCCCATCCTCTTTATTTTCTCTTACCTCGCTGAATTTTTTGTTTCTTCGCTACAATCAATTCTCAGGAAATTTGAGAACATTCCCTGTTTTCTTGAGAAATCAGACCCAATTGGTCTTTTTGGACCTTTCAGATAACCAGATACAGGGCGGGGTACCTAATTGGATGGGGAAGCTTGATCTTGTTTCACTAAATCTTTCATGCAACTCCCTAGATACTTTAGAAGCTTCTTCAGTATCCAATCTCACTTCTCTGAGTTTACTCGACCTCCATTCCAATAAATTTCAAGGACAAATCCCAACTGTCTCATCACCATACGTTTATTATCTGGATTACTCGAGGAATGGTTTCAACTCCAACATACCAACTACAATTGGAGATACGCTTCTTGGAACTGCATTCATTTCTCTTTCGAGCAATAACCTCTATGGGATCATTCCAAGATCAATATGCAAATCACGGAGTCTTGAGGTTCTTGATCTGTCCAATAATTCTCTGAGTGGTACGATTCCTCAGTGTTTGACTGCAATGACCACTCTTGCAGTACTTAATTTAAGGAGAAACAATCTTACAGAGGTTGGCATGTTGTCCAAGAATTGCAGTTTAGAAACTCTCGACATTAGTGAAAATCATATACAAGGCCAGTTTCTAAAATCTCTCGTCAACTGCACAAAGTTGGAGGTTTTAAACCTTGGAAACAATCAGATAACATATCCTTTTCCTTGCTTTTTGAAGAACACATCCACTTTGCGTGTCCTTATCTTACGTTCAAATGAGTTTTATGGACACATTGGATGTCCAGAGACTAATGGAACCTGGCCAGTGCTTCAAATCATTGACCTAGCCAACAACAATTTCAATGGTGAAATACCTGCAAAAGCTTTGATAACGTGGCATGCAATGATGGCTAAACAAGATGATCCCCCATCAAAGCTCAATCACCTTCAATTTCAAGATGATGAAGATGATCGGATTTATTACCAGGATACGATTACAGTTATCAACAAAGGTTTAGAGATGGAGCTGGTAAAGATTCTAAGTGTCTTCACCTCGATTGACTTCTCATGCAACAAGTTCGACGGATCAATACCAAAGGAAATTGGAGAACTCAAATCTCTGTATGCCCTCAACTTGTCAAATAATGTTTTCATAGGAGCAATTCCAGCATCCTTAAGTAGCTTGAGTCAACTAGAGTCGCTAGACATCTCAAAGAACTACCTGAGCGGGCAAATTCCACCACAGCTTACACAGCTCACTTTCCTTTCATTTTTGAATCTTTCATATAATCAACTTGTGGGAAGGATTCCAAGCGAGCAATCACTGGGAAGTTGGGAGAAGCACGGGGTTGCGCTAAAGCGTGACTCTAGCTTCGCTGGGTGCGAGGGCGTTACCCTTGCTTCGCTGGAAGTAACAGTGGCAGTTGCGCTTGCAGTCGGCTCGCTGGGAGACTGGGACTGGAAGTACGGTCGCCGGGTTGATCTGGTGATGCTAGCAGTCGGCTCGCTGGGAGACTGGGACTGTATCGCGGTCACCGGGGTTCAACGAGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

912

Amino Acids

99.69

Weight (kDa)

5.29

Isoelectric Point (pI)

39.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 31 - 69 3.6e-09 Leucine rich repeat N-terminal domain
LRR_8 PF13855 230 - 288 4.5e-06 Leucine rich repeat
LRR_14 PF23598 302 - 490 2.6e-09 Leucine-rich repeat region
LRR_8 PF13855 531 - 586 8.6e-07 Leucine rich repeat
LRR_4 PF12799 597 - 633 6.3e-06 Leucine Rich repeats (2 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 82, 2068
Acc65I GGTACC 1 cut(s) 1342
AccB1I GGYRCC 1 cut(s) 1342
AccBSI CCGCTC 2 cut(s) 342, 2412
AccII CGCG 1 cut(s) 2714
AciI CCGC 5 cut(s) 240, 340, 1338, 2412, 2714
AclWI GGATC 5 cut(s) 910, 937, 1628, 2102, 2278
AcoI YGGCCR 3 cut(s) 337, 1156, 2006
AcuI CTGAAG 2 cut(s) 668, 1404
AdeI CACNNNGTG 1 cut(s) 332
AfaI GTAC 5 cut(s) 172, 1344, 1693, 1734, 2651
AfiI CCNNNNNNNGG 5 cut(s) 65, 313, 737, 899, 1333
AflII CTTAAG 2 cut(s) 374, 2359
AflIII ACRYGT 2 cut(s) 217, 1065
AjiI CACGTC 1 cut(s) 218
AjnI CCWGG 5 cut(s) 449, 591, 736, 2003, 2169
AjuI GAANNNNNNNTTGG 4 cut(s) 414, 446, 2027, 2059
Alw21I GWGCWC 1 cut(s) 537
Alw26I GTCTC 8 cut(s) 78, 284, 693, 782, 1498, 1985, 2628, 2691
AlwI GGATC 5 cut(s) 910, 937, 1628, 2102, 2278
Ama87I CYCGRG 2 cut(s) 253, 1525
AoxI GGCC 9 cut(s) 337, 418, 589, 594, 655, 1110, 1156, 1828, 2006
ApeKI GCWGC 2 cut(s) 69, 204
ArsI GACNNNNNNTTYG 2 cut(s) 2540, 2572
Asp700I GAANNNNTTC 1 cut(s) 1535
Asp718I GGTACC 1 cut(s) 1342
AspLEI GCGC 2 cut(s) 2539, 2614
AspS9I GGNCC 8 cut(s) 418, 589, 656, 876, 912, 1016, 1110, 1312
AsuC2I CCSGG 2 cut(s) 2660, 2722
AsuHPI GGTGA 8 cut(s) 344, 469, 1491, 2063, 2120, 2227, 2683, 2710
AsuII TTCGAA 1 cut(s) 520
AsuNHI GCTAGC 1 cut(s) 2677
AvaI CYCGRG 2 cut(s) 253, 1525
AvaII GGWCC 4 cut(s) 876, 912, 1016, 1312
BalI TGGCCA 1 cut(s) 2008
BanI GGYRCC 1 cut(s) 1342
BanII GRGCYC 2 cut(s) 500, 537
BbsI GAAGAC 1 cut(s) 2224
Bbv12I GWGCWC 1 cut(s) 537
BbvCI CCTCAGC 1 cut(s) 14
BbvI GCAGC 2 cut(s) 81, 216
BcgI CGANNNNNNTGC 8 cut(s) 61, 95, 2245, 2279, 2607, 2641, 2670, 2704
BciT130I CCWGG 5 cut(s) 451, 593, 738, 2005, 2171
BciVI GTATCC 2 cut(s) 1435, 2167
BcnI CCSGG 2 cut(s) 2660, 2722
BcoDI GTCTC 8 cut(s) 78, 284, 693, 782, 1498, 1985, 2628, 2691
BfaI CTAG 8 cut(s) 138, 629, 1400, 2030, 2378, 2387, 2553, 2678
BfrI CTTAAG 2 cut(s) 374, 2359
BfuAI ACCTGC 2 cut(s) 82, 2068
BfuI GTATCC 2 cut(s) 1435, 2167
BglII AGATCT 1 cut(s) 816
BisI GCNGC 3 cut(s) 70, 205, 340
BlpI GCTNAGC 1 cut(s) 678
BlsI GCNGC 3 cut(s) 71, 206, 341
BmcAI AGTACT 1 cut(s) 1734
Bme1390I CCNGG 7 cut(s) 451, 593, 738, 2005, 2171, 2660, 2722
Bme18I GGWCC 4 cut(s) 876, 912, 1016, 1312
BmeT110I CYCGRG 2 cut(s) 253, 1525
BmgBI CACGTC 1 cut(s) 218
BmgT120I GGNCC 8 cut(s) 418, 589, 656, 876, 912, 1016, 1110, 1312
BmiI GGNNCC 4 cut(s) 590, 877, 1344, 2002
BmrFI CCNGG 7 cut(s) 451, 593, 738, 2005, 2171, 2660, 2722
BmrI ACTGGG 3 cut(s) 2519, 2647, 2710
BmsI GCATC 4 cut(s) 121, 852, 2363, 2664
BmtI GCTAGC 1 cut(s) 2681
BmuI ACTGGG 3 cut(s) 2519, 2647, 2710
BpiI GAAGAC 1 cut(s) 2224
BpmI CTGGAG 2 cut(s) 330, 1142
Bpu10I CCTNAGC 2 cut(s) 14, 2408
Bpu1102I GCTNAGC 1 cut(s) 678
Bpu14I TTCGAA 1 cut(s) 520
BpuEI CTTGAG 4 cut(s) 512, 1303, 1676, 2389
BpuMI CCSGG 2 cut(s) 2660, 2722
BsaI GGTCTC 2 cut(s) 78, 782
BsaJI CCNNGG 3 cut(s) 871, 1879, 2721
BsaWI WCCGGW 1 cut(s) 909
BsaXI ACNNNNNCTCC 2 cut(s) 1440, 1470
Bsc4I CCNNNNNNNGG 5 cut(s) 65, 313, 737, 899, 1333
Bse118I RCCGGY 1 cut(s) 1158
Bse1I ACTGG 9 cut(s) 347, 1018, 1159, 1831, 2009, 2514, 2642, 2648, 2705
Bse3DI GCAATG 4 cut(s) 214, 981, 1722, 2094
BseBI CCWGG 5 cut(s) 451, 593, 738, 2005, 2171
BseDI CCNNGG 3 cut(s) 871, 1879, 2721
BseGI GGATG 5 cut(s) 1193, 1359, 1928, 1988, 2354
BseLI CCNNNNNNNGG 5 cut(s) 65, 313, 737, 899, 1333
BseMI GCAATG 4 cut(s) 214, 981, 1722, 2094
BseMII CTCAG 8 cut(s) 28, 793, 867, 1266, 1433, 1676, 1715, 2399
BseNI ACTGG 9 cut(s) 347, 1018, 1159, 1831, 2009, 2514, 2642, 2648, 2705
BseXI GCAGC 2 cut(s) 81, 216
BseYI CCCAGC 3 cut(s) 2561, 2629, 2692
BsgI GTGCAG 2 cut(s) 711, 1840
Bsh1236I CGCG 1 cut(s) 2714
Bsh1285I CGRYCG 1 cut(s) 2656
BshFI GGCC 9 cut(s) 339, 420, 591, 596, 657, 1112, 1158, 1830, 2008
BshNI GGYRCC 1 cut(s) 1342
BsiEI CGRYCG 1 cut(s) 2656
BsiHKAI GWGCWC 1 cut(s) 537
BsiHKCI CYCGRG 2 cut(s) 253, 1525
BsiSI CCGG 4 cut(s) 910, 1159, 2659, 2721
BslFI GGGAC 3 cut(s) 655, 2654, 2717
BslI CCNNNNNNNGG 5 cut(s) 65, 313, 737, 899, 1333
BsmAI GTCTC 8 cut(s) 78, 284, 693, 782, 1498, 1985, 2628, 2691
BsmFI GGGAC 3 cut(s) 655, 2654, 2717
BsmI GAATGC 1 cut(s) 1586
BsnI GGCC 9 cut(s) 339, 420, 591, 596, 657, 1112, 1158, 1830, 2008
Bso31I GGTCTC 2 cut(s) 78, 782
BsoBI CYCGRG 2 cut(s) 253, 1525
Bsp119I TTCGAA 1 cut(s) 520
Bsp1286I GDGCHC 2 cut(s) 500, 537
Bsp1720I GCTNAGC 1 cut(s) 678
Bsp19I CCATGG 1 cut(s) 871
BspACI CCGC 5 cut(s) 240, 340, 1338, 2412, 2714
BspANI GGCC 9 cut(s) 339, 420, 591, 596, 657, 1112, 1158, 1830, 2008
BspCNI CTCAG 8 cut(s) 27, 792, 866, 1265, 1434, 1677, 1714, 2400
BspFNI CGCG 1 cut(s) 2714
BspLI GGNNCC 4 cut(s) 590, 877, 1344, 2002
BspMI ACCTGC 2 cut(s) 82, 2068
BspOI GCTAGC 1 cut(s) 2681
BspPI GGATC 5 cut(s) 910, 937, 1628, 2102, 2278
BspT104I TTCGAA 1 cut(s) 520
BspT107I GGYRCC 1 cut(s) 1342
BspTI CTTAAG 2 cut(s) 374, 2359
BspTNI GGTCTC 2 cut(s) 78, 782
BsrBI CCGCTC 2 cut(s) 342, 2412
BsrDI GCAATG 4 cut(s) 214, 981, 1722, 2094
BsrFI RCCGGY 1 cut(s) 1158
BsrI ACTGG 9 cut(s) 347, 1018, 1159, 1831, 2009, 2514, 2642, 2648, 2705
BssAI RCCGGY 1 cut(s) 1158
BssECI CCNNGG 3 cut(s) 871, 1879, 2721
BssT1I CCWWGG 2 cut(s) 871, 1879
Bst2UI CCWGG 5 cut(s) 451, 593, 738, 2005, 2171
Bst4CI ACNGT 7 cut(s) 988, 1002, 1492, 2185, 2602, 2654, 2708
Bst6I CTCTTC 2 cut(s) 287, 615
BstAFI CTTAAG 2 cut(s) 374, 2359
BstAPI GCANNNNNTGC 1 cut(s) 201
BstBI TTCGAA 1 cut(s) 520
BstC8I GCNNGC 7 cut(s) 2085, 2414, 2501, 2616, 2627, 2679, 2690
BstDSI CCRYGG 1 cut(s) 871
BstEII GGTNACC 1 cut(s) 2716
BstF5I GGATG 5 cut(s) 1193, 1359, 1928, 1988, 2354
BstFNI CGCG 1 cut(s) 2714
BstHHI GCGC 2 cut(s) 2539, 2614
BstMAI GTCTC 8 cut(s) 78, 284, 693, 782, 1498, 1985, 2628, 2691
BstMCI CGRYCG 1 cut(s) 2656
BstMWI GCNNNNNNNGC 5 cut(s) 201, 323, 2093, 2351, 2437
BstNI CCWGG 5 cut(s) 451, 593, 738, 2005, 2171
BstNSI RCATGY 5 cut(s) 54, 204, 1069, 1774, 2087
BstPI GGTNACC 1 cut(s) 2716
BstSCI CCNGG 7 cut(s) 449, 591, 736, 2003, 2169, 2658, 2720
BstUI CGCG 1 cut(s) 2714
BstV1I GCAGC 2 cut(s) 81, 216
BstV2I GAAGAC 1 cut(s) 2224
BstX2I RGATCY 2 cut(s) 816, 902
BstXI CCANNNNNNTGG 2 cut(s) 1033, 1120
BstYI RGATCY 2 cut(s) 816, 902
BsuI GTATCC 2 cut(s) 1435, 2167
BsuRI GGCC 9 cut(s) 339, 420, 591, 596, 657, 1112, 1158, 1830, 2008
BtgI CCRYGG 1 cut(s) 871
BtrI CACGTC 1 cut(s) 218
BtsCI GGATG 5 cut(s) 1193, 1359, 1928, 1988, 2354
BtsI GCAGTG 1 cut(s) 833
BtsIMutI CAGTG 9 cut(s) 702, 833, 998, 1011, 1171, 1709, 2016, 2507, 2607
BveI ACCTGC 2 cut(s) 82, 2068
Cac8I GCNNGC 7 cut(s) 2085, 2414, 2501, 2616, 2627, 2679, 2690
CfoI GCGC 2 cut(s) 2539, 2614
Cfr10I RCCGGY 1 cut(s) 1158
Cfr13I GGNCC 8 cut(s) 418, 589, 656, 876, 912, 1016, 1110, 1312
CsiI ACCWGGT 1 cut(s) 449
Csp6I GTAC 5 cut(s) 171, 1343, 1692, 1733, 2650
CviAII CATG 9 cut(s) 51, 201, 665, 872, 1066, 1389, 1771, 2084, 2253
CviQI GTAC 5 cut(s) 171, 1343, 1692, 1733, 2650
DraI TTTAAA 2 cut(s) 1143, 1875
DraIII CACNNNGTG 1 cut(s) 332
EaeI YGGCCR 3 cut(s) 337, 1156, 2006
Eam1104I CTCTTC 2 cut(s) 287, 615
EarI CTCTTC 2 cut(s) 287, 615
Ecl136II GAGCTC 1 cut(s) 535
Eco130I CCWWGG 2 cut(s) 871, 1879
Eco24I GRGCYC 2 cut(s) 500, 537
Eco31I GGTCTC 2 cut(s) 78, 782
Eco32I GATATC 1 cut(s) 994
Eco47I GGWCC 4 cut(s) 876, 912, 1016, 1312
Eco53kI GAGCTC 1 cut(s) 535
Eco57I CTGAAG 2 cut(s) 668, 1404
Eco88I CYCGRG 2 cut(s) 253, 1525
Eco91I GGTNACC 1 cut(s) 2716
EcoICRI GAGCTC 1 cut(s) 535
EcoO109I RGGNCCY 2 cut(s) 656, 876
EcoO65I GGTNACC 1 cut(s) 2716
EcoRII CCWGG 5 cut(s) 449, 591, 736, 2003, 2169
EcoRV GATATC 1 cut(s) 994
EcoT14I CCWWGG 2 cut(s) 871, 1879
EcoT38I GRGCYC 2 cut(s) 500, 537
ErhI CCWWGG 2 cut(s) 871, 1879
FaeI CATG 9 cut(s) 54, 204, 668, 875, 1069, 1392, 1774, 2087, 2256
FaqI GGGAC 3 cut(s) 655, 2654, 2717
FatI CATG 9 cut(s) 50, 200, 664, 871, 1065, 1388, 1770, 2083, 2252
FauI CCCGC 2 cut(s) 1331, 2405
Fnu4HI GCNGC 3 cut(s) 70, 205, 340
FokI GGATG 5 cut(s) 1180, 1366, 1915, 1995, 2341
FriOI GRGCYC 2 cut(s) 500, 537
Fsp4HI GCNGC 3 cut(s) 70, 205, 340
FspBI CTAG 8 cut(s) 138, 629, 1400, 2030, 2378, 2387, 2553, 2678
GlaI GCGC 2 cut(s) 2538, 2613
GluI GCNGC 3 cut(s) 70, 205, 340
GsaI CCCAGC 3 cut(s) 2565, 2633, 2696
GsuI CTGGAG 2 cut(s) 330, 1142
HaeIII GGCC 9 cut(s) 339, 420, 591, 596, 657, 1112, 1158, 1830, 2008
HapII CCGG 4 cut(s) 910, 1159, 2659, 2721
HhaI GCGC 2 cut(s) 2539, 2614
Hin1II CATG 9 cut(s) 54, 204, 668, 875, 1069, 1392, 1774, 2087, 2256
Hin6I GCGC 2 cut(s) 2537, 2612
HinP1I GCGC 2 cut(s) 2537, 2612
HincII GTYRAC 3 cut(s) 232, 1852, 2375
HindII GTYRAC 3 cut(s) 232, 1852, 2375
HindIII AAGCTT 5 cut(s) 149, 680, 1361, 1413, 2067
HpaII CCGG 4 cut(s) 910, 1159, 2659, 2721
HphI GGTGA 8 cut(s) 344, 469, 1491, 2063, 2120, 2227, 2683, 2710
Hpy166II GTNNAC 5 cut(s) 232, 984, 1448, 1852, 2375
Hpy8I GTNNAC 5 cut(s) 232, 984, 1448, 1852, 2375
Hpy99I CGWCG 2 cut(s) 148, 2270
HpyAV CCTTC 5 cut(s) 308, 1079, 1175, 2141, 2481
HpyCH4III ACNGT 7 cut(s) 988, 1002, 1492, 2185, 2602, 2654, 2708
HpyCH4IV ACGT 4 cut(s) 217, 1506, 1954, 2078
HpyF10VI GCNNNNNNNGC 5 cut(s) 201, 323, 2093, 2351, 2437
HpySE526I ACGT 4 cut(s) 217, 1506, 1954, 2078
Hsp92II CATG 9 cut(s) 54, 204, 668, 875, 1069, 1392, 1774, 2087, 2256
HspAI GCGC 2 cut(s) 2537, 2612
KpnI GGTACC 1 cut(s) 1346
LmnI GCTCC 3 cut(s) 59, 2209, 2342
Lsp1109I GCAGC 2 cut(s) 81, 216
LweI GCATC 4 cut(s) 121, 852, 2363, 2664
MabI ACCWGGT 1 cut(s) 449
MaeI CTAG 8 cut(s) 138, 629, 1400, 2030, 2378, 2387, 2553, 2678
MaeII ACGT 4 cut(s) 217, 1506, 1954, 2078
MaeIII GTNAC 7 cut(s) 46, 948, 1166, 2546, 2575, 2596, 2716
MbiI CCGCTC 2 cut(s) 342, 2412
MboII GAAGA 9 cut(s) 274, 281, 597, 602, 1226, 1410, 1933, 2162, 2224
MfeI CAATTG 5 cut(s) 63, 640, 704, 1298, 1560
MflI RGATCY 2 cut(s) 816, 902
MhlI GDGCHC 2 cut(s) 500, 537
MlsI TGGCCA 1 cut(s) 2008
MluNI TGGCCA 1 cut(s) 2008
MlyI GAGTC 5 cut(s) 677, 1660, 2380, 2390, 2543
MmeI TCCRAC 2 cut(s) 1569, 1845
Mox20I TGGCCA 1 cut(s) 2008
MroXI GAANNNNTTC 1 cut(s) 1535
MscI TGGCCA 1 cut(s) 2008
MseI TTAA 9 cut(s) 375, 485, 552, 1056, 1142, 1737, 1742, 1874, 2360
Msp20I TGGCCA 1 cut(s) 2008
MspA1I CMGCKG 1 cut(s) 240
MspCI CTTAAG 2 cut(s) 374, 2359
MspI CCGG 4 cut(s) 910, 1159, 2659, 2721
MspR9I CCNGG 7 cut(s) 451, 593, 738, 2005, 2171, 2660, 2722
MunI CAATTG 5 cut(s) 63, 640, 704, 1298, 1560
Mva1269I GAATGC 1 cut(s) 1586
MvaI CCWGG 5 cut(s) 451, 593, 738, 2005, 2171
MvnI CGCG 1 cut(s) 2714
MwoI GCNNNNNNNGC 5 cut(s) 201, 323, 2093, 2351, 2437
NciI CCSGG 2 cut(s) 2660, 2722
NcoI CCATGG 1 cut(s) 871
NheI GCTAGC 1 cut(s) 2677
NlaIII CATG 9 cut(s) 54, 204, 668, 875, 1069, 1392, 1774, 2087, 2256
NlaIV GGNNCC 4 cut(s) 590, 877, 1344, 2002
NmeAIII GCCGAG 1 cut(s) 171
NmuCI GTSAC 3 cut(s) 1166, 2546, 2716
NspI RCATGY 5 cut(s) 54, 204, 1069, 1774, 2087
NspV TTCGAA 1 cut(s) 520
PaeI GCATGC 1 cut(s) 2087
PaeR7I CTCGAG 1 cut(s) 1525
PciI ACATGT 1 cut(s) 1065
PcsI WCGNNNNNNNCGW 1 cut(s) 2565
PctI GAATGC 1 cut(s) 1586
PdmI GAANNNNTTC 1 cut(s) 1535
PfeI GAWTC 9 cut(s) 164, 245, 460, 859, 1117, 1696, 2221, 2461, 2491
PkrI GCNGC 3 cut(s) 71, 206, 341
PleI GAGTC 5 cut(s) 676, 1659, 2379, 2389, 2543
PpsI GAGTC 5 cut(s) 676, 1659, 2379, 2389, 2543
PpuMI RGGWCCY 1 cut(s) 876
PscI ACATGT 1 cut(s) 1065
Psp124BI GAGCTC 1 cut(s) 537
Psp5II RGGWCCY 1 cut(s) 876
Psp6I CCWGG 5 cut(s) 449, 591, 736, 2003, 2169
PspEI GGTNACC 1 cut(s) 2716
PspFI CCCAGC 3 cut(s) 2561, 2629, 2692
PspGI CCWGG 5 cut(s) 449, 591, 736, 2003, 2169
PspN4I GGNNCC 4 cut(s) 590, 877, 1344, 2002
PspPI GGNCC 8 cut(s) 418, 589, 656, 876, 912, 1016, 1110, 1312
PspPPI RGGWCCY 1 cut(s) 876
PspXI VCTCGAGB 1 cut(s) 1525
PsuI RGATCY 2 cut(s) 816, 902
RsaI GTAC 5 cut(s) 172, 1344, 1693, 1734, 2651
RsaNI GTAC 5 cut(s) 171, 1343, 1692, 1733, 2650
SacI GAGCTC 1 cut(s) 537
SaqAI TTAA 9 cut(s) 375, 485, 552, 1056, 1142, 1737, 1742, 1874, 2360
SatI GCNGC 3 cut(s) 70, 205, 340
Sau96I GGNCC 8 cut(s) 418, 589, 656, 876, 912, 1016, 1110, 1312
ScaI AGTACT 1 cut(s) 1734
SchI GAGTC 5 cut(s) 677, 1660, 2380, 2390, 2543
ScrFI CCNGG 7 cut(s) 451, 593, 738, 2005, 2171, 2660, 2722
SduI GDGCHC 2 cut(s) 500, 537
SexAI ACCWGGT 1 cut(s) 449
SfaNI GCATC 4 cut(s) 121, 852, 2363, 2664
Sfr274I CTCGAG 1 cut(s) 1525
SfuI TTCGAA 1 cut(s) 520
SinI GGWCC 4 cut(s) 876, 912, 1016, 1312
SlaI CTCGAG 1 cut(s) 1525
SmlI CTYRAG 7 cut(s) 374, 491, 1282, 1525, 1655, 2359, 2368
SmoI CTYRAG 7 cut(s) 374, 491, 1282, 1525, 1655, 2359, 2368
SphI GCATGC 1 cut(s) 2087
SsiI CCGC 5 cut(s) 240, 340, 1338, 2412, 2714
SspI AATATT 1 cut(s) 804
SspMI CTAG 8 cut(s) 138, 629, 1400, 2030, 2378, 2387, 2553, 2678
SstI GAGCTC 1 cut(s) 537
StyD4I CCNGG 7 cut(s) 449, 591, 736, 2003, 2169, 2658, 2720
StyI CCWWGG 2 cut(s) 871, 1879
TaaI ACNGT 7 cut(s) 988, 1002, 1492, 2185, 2602, 2654, 2708
TaiI ACGT 4 cut(s) 220, 1509, 1957, 2081
TatI WGTACW 2 cut(s) 170, 1732
TauI GCSGC 1 cut(s) 342
TfiI GAWTC 9 cut(s) 164, 245, 460, 859, 1117, 1696, 2221, 2461, 2491
Tru1I TTAA 9 cut(s) 375, 485, 552, 1056, 1142, 1737, 1742, 1874, 2360
Tru9I TTAA 9 cut(s) 375, 485, 552, 1056, 1142, 1737, 1742, 1874, 2360
TscAI CASTG 9 cut(s) 702, 840, 1005, 1018, 1171, 1709, 2016, 2514, 2607
TseFI GTSAC 3 cut(s) 1166, 2546, 2716
TseI GCWGC 2 cut(s) 69, 204
Tsp45I GTSAC 3 cut(s) 1166, 2546, 2716
TspDTI ATGAA 9 cut(s) 17, 429, 570, 1377, 1579, 2163, 2326, 2442, 2457
TspGWI ACGGA 3 cut(s) 34, 1663, 2283
TspRI CASTG 9 cut(s) 702, 840, 1005, 1018, 1171, 1709, 2016, 2514, 2607
Vha464I CTTAAG 2 cut(s) 374, 2359
VpaK11BI GGWCC 4 cut(s) 876, 912, 1016, 1312
XceI RCATGY 5 cut(s) 54, 204, 1069, 1774, 2087
XhoI CTCGAG 1 cut(s) 1525
XmnI GAANNNNTTC 1 cut(s) 1535
XspI CTAG 8 cut(s) 138, 629, 1400, 2030, 2378, 2387, 2553, 2678
ZrmI AGTACT 1 cut(s) 1734
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.