Rorug04G0259000

Protein terminal ear1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0009258
Physical Location & Seq
Reverse (-)
43183353 .. 43186073
2721 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0259000.1

Sequence Viewer

Length: 2154 bp
ATGGACTCGTCAAAGCCTTATGTTCGACTCTCTGCAGCACAAGACAACTCAGGAGCTTGGAACCCACAACAGAAGCTTGCAGTTTCTAGTTCATCTTCACTTTCAGGTTCTCTTCCTATCCGAGCTCGTCTTACTCTAGATTCTGATTCTGACTCTGAAATCAACGGTGGTCATGAATTGGGTTATATTACTAGTAGTAGTATTAGTGTTGGTTCCGAGAGTGGGTTTGTGAGTGGGGAAGAGTTTGAAACGGCGTCAGAGAGGCCCTTTGTGGCGGTGGAGTCGGGTGAGGAAGCCCTTGAAGAGAGTGGCTCTGTTGAAAAATATGAGGTTTTTTGGCCTTCTGTGTCTGACCCAGATGAATATGAAGAAGCTGTTGTGGGTAAAGTTATGGTGGGTGGTCCAATTTCGGAGGAGGTTAGGCCCATTGCAAATTTGTCATGGGAGAGTGATGGGAGTGATGTAGTGAGTGAGGAGACTGAAGAAGTGAGTGAAGATGAAGGTGGTGGATTGTTGGGTCATGTTAAGGCTCTGGTTTATGGGTTTGTGGAGCAGGTTGGTATTCCACCTAGAGTTAAGGTTTCTGAGGTTGATGAAGGAGAAGAAGATGAGTCCTTGGTTGTTAAAGAATCAAGTTTTGTTGAAGGTGGAAATGGAGATTCTGTTCAAGTGGAATGCGGTGAGGATTGTAGTTTTGATACTGCTGAGTCCAGTGAAACGAACTCTGTTGAGTTGAATTTGAATGTATTGGAAGGTGGAGAGAAAGATGAGTATGTTGAAGAAGAGTTCTTTGTGCATGAAAGTTCCGTGAAATTGGATGTTGAGGGTAACGAGAATCTGAGTATGGGAAATAGTCATATATCTGAAGCTGAGGAAGATAAAGTTGAATATAGTGATTCCTTTAACTCAATCACACCAAGGTCTGTCTTAGGTGAGAATGTGGCCACTGAACTCGAGATTGAGGATCCGATTGCTAATGGGAAGGACTTGGTTTTTACTGGAGATGCTGGTGTGAAATATGAAGCCAAAGTGGAAGTCCCTCTCAAGGAGAAGAAGGGTTACACTATGGAACAGCACCCAACCAGCTTGGGTCTGTTTGTGGATAGTGTTGCACATGGGTTTCAGGTTTATGAGGTTGAAAAAGAAGTAGAAGAAGAACACAAGTTAGTGATGGGGAAAGGCTCCTCCGATTCCTTCATGAAGCAGTCTGAGCCGGCCACAGAATTAAAGGGTGTTCAGATTTCAGACTTGAAAGCCATTGTTAAAGGTCAAACTGGGGAATCCATTCAAGCTGAATGGACTGAAGATAATGGTCTTGGTGGTGTGGAAACCAAGGAACCAGATATTTTGGAGAAAGCCAAGAGTATGGCAGAAGAGTCTATAATGCCTTCCCCAAAAAGCAAAACGAGTGATGTTGATATTAGCGAAAGTAATGGGGCATTGGATAGAGGAGTCCATTCAGAATGTGATTTTGATGTTGAGGGGAGTGGAAATCAGATAACGGACTCCAATTCATATTCACAGCCAAATCCAATTCTGAAGATCGTATATGTGAGTAAGCGCAATTTGTCTGAAGCTGCTGGGGGTGATGGATATGTACTGTGTTCTGATTTCCTTGAATCAATCACACCAAGTTCCAGTGTGACCCATGAACACATTTTTGGTGGTCAAACTAAGGTAAAAAATTGTAAAATAGGCAGAAGATTCACTGGAGTAACCGTAACTCACTTGGGTGAGGATGCCACCACCGGACTCAAGATTGAGGATCAGATTGCTATTGGGAAGGGCTTGGTGATGGCTGGAAGTGCTGGAGCTGTGCAATGCCAAGGTGATACAGCATGTGGAGCCAAACTGGAATTGTGCCTCAAGAAGAAGAATTTCCCTATAAAGCAGGACCAAACTAGCTTGGGTCTGTCTTTGATGAAGTGGGGTGGTGGTCTTCTTGTAACAACGGATATACTGTCTCGGTTCTCTGTTGGGAGTTCTAAAATAGCTGTTCATGTTGTATTGGACAACAAGCAGAGGGGAAAGATTGCTATAAGAACCAGAAACTCCGATAAAATCCCAAGTGCAGGGATTATTGGGATTCTCTCAATTGCAGTTTCAGTCTTCGGGTTCTTCCGTTCAAGATTCGGTGTTCAGAACTCAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1 RRM_2

Protein Analysis

717

Amino Acids

76.84

Weight (kDa)

4.48

Isoelectric Point (pI)

39.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOC159_MAD PF11886 553 - 703 1.4e-37 Translocase of chloroplast 159/132, membrane anchor domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 544
AciI CCGC 2 cut(s) 275, 678
AclWI GGATC 3 cut(s) 959, 972, 1773
AcoI YGGCCR 2 cut(s) 942, 1215
AcsI RAATTY 3 cut(s) 433, 736, 1876
AcuI CTGAAG 5 cut(s) 501, 885, 1323, 1559, 1593
AcyI GRCGYC 1 cut(s) 254
AfaI GTAC 1 cut(s) 1599
AfiI CCNNNNNNNGG 3 cut(s) 222, 1045, 2072
AhlI ACTAGT 1 cut(s) 191
AjuI GAANNNNNNNTTGG 2 cut(s) 1644, 1676
AleI CACNNNNGTG 1 cut(s) 1731
AloI GAACNNNNNNTCC 2 cut(s) 648, 680
Alw21I GWGCWC 1 cut(s) 127
Alw26I GTCTC 2 cut(s) 470, 1968
AlwI GGATC 3 cut(s) 959, 972, 1773
Ama87I CYCGRG 1 cut(s) 953
AoxI GGCC 5 cut(s) 263, 338, 422, 942, 1215
ApeKI GCWGC 2 cut(s) 35, 1577
ApoI RAATTY 3 cut(s) 433, 736, 1876
ArsI GACNNNNNNTTYG 2 cut(s) 1020, 1052
Asp700I GAANNNNTTC 2 cut(s) 1284, 1877
AspLEI GCGC 1 cut(s) 1563
AspS9I GGNCC 4 cut(s) 264, 401, 423, 1894
AsuHPI GGTGA 7 cut(s) 299, 692, 944, 1598, 1745, 1804, 1841
AvaI CYCGRG 1 cut(s) 953
AvaII GGWCC 2 cut(s) 401, 1894
BalI TGGCCA 1 cut(s) 944
BamHI GGATCC 1 cut(s) 964
BanII GRGCYC 1 cut(s) 127
BarI GAAGNNNNNNTAC 2 cut(s) 1043, 1075
BbsI GAAGAC 2 cut(s) 1931, 2101
Bbv12I GWGCWC 1 cut(s) 127
BbvCI CCTCAGC 1 cut(s) 870
BbvI GCAGC 2 cut(s) 47, 1564
BccI CCATC 4 cut(s) 446, 1165, 1583, 1789
BceAI ACGGC 1 cut(s) 267
BcoDI GTCTC 2 cut(s) 470, 1968
BcuI ACTAGT 1 cut(s) 191
BfaI CTAG 5 cut(s) 87, 137, 192, 570, 1902
BfmI CTRYAG 1 cut(s) 33
BfuAI ACCTGC 1 cut(s) 544
BisI GCNGC 2 cut(s) 36, 1578
BlsI GCNGC 2 cut(s) 37, 1579
Bme18I GGWCC 2 cut(s) 401, 1894
BmeT110I CYCGRG 1 cut(s) 953
BmgT120I GGNCC 4 cut(s) 264, 401, 423, 1894
BmiI GGNNCC 6 cut(s) 62, 214, 966, 1183, 1338, 1846
BmrI ACTGGG 1 cut(s) 1284
BmsI GCATC 2 cut(s) 994, 1729
BmuI ACTGGG 1 cut(s) 1284
BpiI GAAGAC 2 cut(s) 1931, 2101
BplI GAGNNNNNCTC 2 cut(s) 296, 328
BpmI CTGGAG 3 cut(s) 1020, 1731, 1830
Bpu10I CCTNAGC 1 cut(s) 870
BpuEI CTTGAG 4 cut(s) 1028, 1739, 1850, 2131
BsaHI GRCGYC 1 cut(s) 254
BsaJI CCNNGG 4 cut(s) 615, 917, 1332, 1825
BsaWI WCCGGW 1 cut(s) 1748
BsaXI ACNNNNNCTCC 4 cut(s) 648, 678, 1704, 1734
Bsc4I CCNNNNNNNGG 3 cut(s) 222, 1045, 2072
Bse118I RCCGGY 1 cut(s) 1213
Bse1I ACTGG 6 cut(s) 711, 1003, 1279, 1638, 1714, 1857
Bse3DI GCAATG 2 cut(s) 426, 1826
BseDI CCNNGG 4 cut(s) 615, 917, 1332, 1825
BseGI GGATG 2 cut(s) 823, 1744
BseLI CCNNNNNNNGG 3 cut(s) 222, 1045, 2072
BseMI GCAATG 2 cut(s) 426, 1826
BseMII CTCAG 6 cut(s) 63, 576, 696, 830, 861, 1200
BseNI ACTGG 6 cut(s) 711, 1003, 1279, 1638, 1714, 1857
BseRI GAGGAG 4 cut(s) 428, 488, 1174, 1464
BseXI GCAGC 2 cut(s) 47, 1564
BseYI CCCAGC 1 cut(s) 1580
BsgI GTGCAG 1 cut(s) 2091
BshFI GGCC 5 cut(s) 265, 340, 424, 944, 1217
BsiHKAI GWGCWC 1 cut(s) 127
BsiHKCI CYCGRG 1 cut(s) 953
BsiSI CCGG 2 cut(s) 1214, 1749
BslFI GGGAC 1 cut(s) 1022
BslI CCNNNNNNNGG 3 cut(s) 222, 1045, 2072
BsmAI GTCTC 2 cut(s) 470, 1968
BsmFI GGGAC 1 cut(s) 1022
BsmI GAATGC 1 cut(s) 680
BsnI GGCC 5 cut(s) 265, 340, 424, 944, 1217
BsoBI CYCGRG 1 cut(s) 953
Bsp1286I GDGCHC 1 cut(s) 127
Bsp143I GATC 3 cut(s) 964, 1542, 1765
BspACI CCGC 2 cut(s) 275, 678
BspANI GGCC 5 cut(s) 265, 340, 424, 944, 1217
BspCNI CTCAG 6 cut(s) 62, 577, 697, 831, 862, 1201
BspHI TCATGA 2 cut(s) 172, 1197
BspLI GGNNCC 6 cut(s) 62, 214, 966, 1183, 1338, 1846
BspMAI CTGCAG 1 cut(s) 37
BspMI ACCTGC 1 cut(s) 544
BspPI GGATC 3 cut(s) 959, 972, 1773
BsrDI GCAATG 2 cut(s) 426, 1826
BsrFI RCCGGY 1 cut(s) 1213
BsrI ACTGG 6 cut(s) 711, 1003, 1279, 1638, 1714, 1857
BssAI RCCGGY 1 cut(s) 1213
BssECI CCNNGG 4 cut(s) 615, 917, 1332, 1825
BssMI GATC 3 cut(s) 964, 1542, 1765
BssNI GRCGYC 1 cut(s) 254
BssT1I CCWWGG 4 cut(s) 615, 917, 1332, 1825
Bst4CI ACNGT 4 cut(s) 167, 1602, 1720, 1962
Bst6I CTCTTC 5 cut(s) 117, 234, 297, 777, 1368
BstACI GRCGYC 1 cut(s) 254
BstC8I GCNNGC 2 cut(s) 78, 1215
BstDEI CTNAG 8 cut(s) 49, 585, 705, 839, 870, 928, 1209, 1674
BstF5I GGATG 2 cut(s) 823, 1744
BstHHI GCGC 1 cut(s) 1563
BstKTI GATC 3 cut(s) 967, 1545, 1768
BstMAI GTCTC 2 cut(s) 470, 1968
BstMBI GATC 3 cut(s) 964, 1542, 1765
BstMWI GCNNNNNNNGC 3 cut(s) 1210, 1805, 1844
BstNSI RCATGY 1 cut(s) 1842
BstSFI CTRYAG 1 cut(s) 33
BstV1I GCAGC 2 cut(s) 47, 1564
BstV2I GAAGAC 2 cut(s) 1931, 2101
BstX2I RGATCY 1 cut(s) 964
BstXI CCANNNNNNTGG 1 cut(s) 1366
BstYI RGATCY 1 cut(s) 964
BsuRI GGCC 5 cut(s) 265, 340, 424, 944, 1217
BtsCI GGATG 2 cut(s) 823, 1744
BtsIMutI CAGTG 4 cut(s) 718, 945, 1645, 1707
BveI ACCTGC 1 cut(s) 544
Cac8I GCNNGC 2 cut(s) 78, 1215
CciI TCATGA 2 cut(s) 172, 1197
CfoI GCGC 1 cut(s) 1563
Cfr10I RCCGGY 1 cut(s) 1213
Cfr13I GGNCC 4 cut(s) 264, 401, 423, 1894
CseI GACGC 1 cut(s) 243
Csp6I GTAC 1 cut(s) 1598
CviAII CATG 9 cut(s) 173, 441, 521, 797, 1115, 1198, 1649, 1839, 2000
CviQI GTAC 1 cut(s) 1598
DdeI CTNAG 8 cut(s) 49, 585, 705, 839, 870, 928, 1209, 1674
DpnI GATC 3 cut(s) 966, 1544, 1767
DpnII GATC 3 cut(s) 964, 1542, 1765
EaeI YGGCCR 2 cut(s) 942, 1215
Eam1104I CTCTTC 5 cut(s) 117, 234, 297, 777, 1368
EarI CTCTTC 5 cut(s) 117, 234, 297, 777, 1368
Ecl136II GAGCTC 1 cut(s) 125
Eco130I CCWWGG 4 cut(s) 615, 917, 1332, 1825
Eco24I GRGCYC 1 cut(s) 127
Eco47I GGWCC 2 cut(s) 401, 1894
Eco53kI GAGCTC 1 cut(s) 125
Eco57I CTGAAG 5 cut(s) 501, 885, 1323, 1559, 1593
Eco88I CYCGRG 1 cut(s) 953
EcoICRI GAGCTC 1 cut(s) 125
EcoO109I RGGNCCY 1 cut(s) 264
EcoT14I CCWWGG 4 cut(s) 615, 917, 1332, 1825
EcoT38I GRGCYC 1 cut(s) 127
ErhI CCWWGG 4 cut(s) 615, 917, 1332, 1825
FaeI CATG 9 cut(s) 176, 444, 524, 800, 1118, 1201, 1652, 1842, 2003
FaqI GGGAC 1 cut(s) 1022
FatI CATG 9 cut(s) 172, 440, 520, 796, 1114, 1197, 1648, 1838, 1999
Fnu4HI GCNGC 2 cut(s) 36, 1578
FokI GGATG 2 cut(s) 830, 1751
FriOI GRGCYC 1 cut(s) 127
Fsp4HI GCNGC 2 cut(s) 36, 1578
FspBI CTAG 5 cut(s) 87, 137, 192, 570, 1902
GlaI GCGC 1 cut(s) 1562
GluI GCNGC 2 cut(s) 36, 1578
GsaI CCCAGC 1 cut(s) 1584
GsuI CTGGAG 3 cut(s) 1020, 1731, 1830
HaeIII GGCC 5 cut(s) 265, 340, 424, 944, 1217
HapII CCGG 2 cut(s) 1214, 1749
HgaI GACGC 1 cut(s) 243
HhaI GCGC 1 cut(s) 1563
Hin1I GRCGYC 1 cut(s) 254
Hin1II CATG 9 cut(s) 176, 444, 524, 800, 1118, 1201, 1652, 1842, 2003
Hin6I GCGC 1 cut(s) 1561
HinP1I GCGC 1 cut(s) 1561
HindIII AAGCTT 1 cut(s) 74
HpaII CCGG 2 cut(s) 1214, 1749
HphI GGTGA 7 cut(s) 299, 692, 944, 1598, 1745, 1804, 1841
Hpy188III TCNNGA 9 cut(s) 51, 137, 173, 955, 1198, 1756, 1867, 2127, 2148
HpyCH4III ACNGT 4 cut(s) 167, 1602, 1720, 1962
HpyCH4V TGCA 8 cut(s) 35, 80, 431, 796, 1112, 1819, 2072, 2099
HpyF10VI GCNNNNNNNGC 3 cut(s) 1210, 1805, 1844
HpyF3I CTNAG 8 cut(s) 49, 585, 705, 839, 870, 928, 1209, 1674
Hsp92I GRCGYC 1 cut(s) 254
Hsp92II CATG 9 cut(s) 176, 444, 524, 800, 1118, 1201, 1652, 1842, 2003
HspAI GCGC 1 cut(s) 1561
KroI GCCGGC 1 cut(s) 1213
KroNI GCCGGC 1 cut(s) 1215
Kzo9I GATC 3 cut(s) 964, 1542, 1765
LmnI GCTCC 5 cut(s) 53, 550, 1187, 1811, 1844
Lsp1109I GCAGC 2 cut(s) 47, 1564
LweI GCATC 2 cut(s) 994, 1729
MaeI CTAG 5 cut(s) 87, 137, 192, 570, 1902
MaeIII GTNAC 6 cut(s) 827, 1058, 1642, 1714, 1720, 1945
MalI GATC 3 cut(s) 966, 1544, 1767
MboI GATC 3 cut(s) 964, 1542, 1765
MfeI CAATTG 1 cut(s) 2094
MflI RGATCY 1 cut(s) 964
MhlI GDGCHC 1 cut(s) 127
MlsI TGGCCA 1 cut(s) 944
MluNI TGGCCA 1 cut(s) 944
MlyI GAGTC 9 cut(s) 21, 146, 290, 620, 716, 1385, 1461, 1499, 1746
Mox20I TGGCCA 1 cut(s) 944
MroNI GCCGGC 1 cut(s) 1213
MroXI GAANNNNTTC 2 cut(s) 1284, 1877
MscI TGGCCA 1 cut(s) 944
MseI TTAA 6 cut(s) 525, 576, 624, 903, 1226, 1263
MslI CAYNNNNRTG 1 cut(s) 1731
Msp20I TGGCCA 1 cut(s) 944
MspI CCGG 2 cut(s) 1214, 1749
MunI CAATTG 1 cut(s) 2094
Mva1269I GAATGC 1 cut(s) 680
MwoI GCNNNNNNNGC 3 cut(s) 1210, 1805, 1844
NaeI GCCGGC 1 cut(s) 1215
NdeII GATC 3 cut(s) 964, 1542, 1765
NgoMIV GCCGGC 1 cut(s) 1213
NlaIII CATG 9 cut(s) 176, 444, 524, 800, 1118, 1201, 1652, 1842, 2003
NlaIV GGNNCC 6 cut(s) 62, 214, 966, 1183, 1338, 1846
NmuCI GTSAC 1 cut(s) 1642
NspI RCATGY 1 cut(s) 1842
OliI CACNNNNGTG 1 cut(s) 1731
PaeR7I CTCGAG 1 cut(s) 953
PagI TCATGA 2 cut(s) 172, 1197
PctI GAATGC 1 cut(s) 680
PdiI GCCGGC 1 cut(s) 1215
PdmI GAANNNNTTC 2 cut(s) 1284, 1877
PkrI GCNGC 2 cut(s) 37, 1579
PleI GAGTC 9 cut(s) 21, 146, 289, 619, 715, 1384, 1460, 1499, 1746
PpsI GAGTC 9 cut(s) 21, 146, 289, 619, 715, 1384, 1460, 1499, 1746
Psp124BI GAGCTC 1 cut(s) 127
PspFI CCCAGC 1 cut(s) 1580
PspN4I GGNNCC 6 cut(s) 62, 214, 966, 1183, 1338, 1846
PspPI GGNCC 4 cut(s) 264, 401, 423, 1894
PstI CTGCAG 1 cut(s) 37
PsuI RGATCY 1 cut(s) 964
RsaI GTAC 1 cut(s) 1599
RsaNI GTAC 1 cut(s) 1598
RseI CAYNNNNRTG 1 cut(s) 1731
SacI GAGCTC 1 cut(s) 127
SaqAI TTAA 6 cut(s) 525, 576, 624, 903, 1226, 1263
SatI GCNGC 2 cut(s) 36, 1578
Sau3AI GATC 3 cut(s) 964, 1542, 1765
Sau96I GGNCC 4 cut(s) 264, 401, 423, 1894
SchI GAGTC 9 cut(s) 21, 146, 290, 620, 716, 1385, 1461, 1499, 1746
SduI GDGCHC 1 cut(s) 127
SfaNI GCATC 2 cut(s) 994, 1729
SfcI CTRYAG 1 cut(s) 33
Sfr274I CTCGAG 1 cut(s) 953
SinI GGWCC 2 cut(s) 401, 1894
SlaI CTCGAG 1 cut(s) 953
SmiMI CAYNNNNRTG 1 cut(s) 1731
SmlI CTYRAG 5 cut(s) 953, 1043, 1754, 1865, 2146
SmoI CTYRAG 5 cut(s) 953, 1043, 1754, 1865, 2146
SpeI ACTAGT 1 cut(s) 191
SsiI CCGC 2 cut(s) 275, 678
SspMI CTAG 5 cut(s) 87, 137, 192, 570, 1902
SstI GAGCTC 1 cut(s) 127
StyI CCWWGG 4 cut(s) 615, 917, 1332, 1825
TaaI ACNGT 4 cut(s) 167, 1602, 1720, 1962
TaqI TCGA 2 cut(s) 25, 954
TatI WGTACW 1 cut(s) 1597
Tru1I TTAA 6 cut(s) 525, 576, 624, 903, 1226, 1263
Tru9I TTAA 6 cut(s) 525, 576, 624, 903, 1226, 1263
TscAI CASTG 4 cut(s) 718, 952, 1645, 1714
TseFI GTSAC 1 cut(s) 1642
TseI GCWGC 2 cut(s) 35, 1577
Tsp45I GTSAC 1 cut(s) 1642
TspGWI ACGGA 4 cut(s) 796, 1517, 1967, 2111
TspRI CASTG 4 cut(s) 718, 952, 1645, 1714
VpaK11BI GGWCC 2 cut(s) 401, 1894
XapI RAATTY 3 cut(s) 433, 736, 1876
XbaI TCTAGA 1 cut(s) 136
XceI RCATGY 1 cut(s) 1842
XhoI CTCGAG 1 cut(s) 953
XmnI GAANNNNTTC 2 cut(s) 1284, 1877
XspI CTAG 5 cut(s) 87, 137, 192, 570, 1902
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.