Rorug05G0037700

TITAN-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0008162
Physical Location & Seq
Reverse (-)
2828690 .. 2871894
43205 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0037700.1

Sequence Viewer

Length: 2505 bp
ATGAGTTTTAGTAGCGCAGCGTTTATCCAGATATTGCATATCTTCCTCCTTCTCCCCTCTAGCATCTGCCAACTTCCCCTTGACACCCTTACGCCAAACCAACCCATCAGAGACGGCGACGTTTTAGTCTCTAGCAGGAAACTCTTTGCACTAGGGTTCTTCAGCCCCGGAAATCCTCAGCACCGCTACGTTGGAGTTTGGTTTTACAAAGTTCCAGAGAAAACCATTGTCTGGGTTGCGAACAGAAACCACCCAGTCAATGATTCCTCTGGAGTGCTATCGATCAGTGGTGATGGAGGCCTTGTCATACACGGAAAGGACCAAAGTACCCCTCTTTGGTCCGCAAACGTTACTCTCTCTTCTCCAAACAATTTCACAGCCAAGCTTCGGGATACAGGAAATCTTGTTTTACTTGAGAATGGAAACCAAAGAGTTGTGTGGGATAGCTTTGATTACCCCTCAGATACAGTGCTTTCCTCTATGAAGCTTGGGCTGAACCGGCGGTCCGGGTTGGAGTGGCTCCTAACGTCTTGGAAGTCAAAAGATGACCCGGGAACCGGGAGCTGTACATATGGGATAGACCCGAGGGGGTCTCCACAGTTGTTTCTGAAAAAGGGGGGAGGCCCTTTGTGGCGGAGCGGCCCTTGGATCGGGGACAGATGGAGCGGTGAACCCCAACTAACCACTACCGTTGGACTCTTCAATTTAAGTTTTGTCAACAATGAAGACGAGATATCCTACAAGCGTGTTGTTGCCAATGACTCATTGTTCACAAGGGGGGTGCTAGATGAATCAGGAATATATCAACGGCTCATGTGGAATGATAAGGAAAACAAATGGATCGAATTGGTCTCCGACCCGAATGAGTGGTGTGATAACTATGGACTCTGTGGTCCAAATAGTAACTGTGACCCATACAATGACTATATGTTTGCTTGCACTTGCCTACCCGGCTTCGAACCCAAATCGCCTCGTGACTGGTATTTGAGAATTGGCGTGGGTGGGTGCATTAGGAAAGCAGGAGTGTCTGTGTGTCGGAACGGAGAAGGGTTCGTGCTGGTGGAGAATGTAAAGGTACCGGACTCATCTACGGCAGTACGTGTGAATATGAGTTTGAGTTTGGAAGCTTGTAAGCTAGAGTGCTTGATGGATTGTTCTTGCACGGCTTACACGAGTGCAGATGAGCGGGGAGGTGGAAATGGGTGCTTGACATGGCACGGGGACTTGATGGACACGAGGACTTACCCCAAAGTTGGTCAAGATTTATATGTTCGAGTTGATGCAACTTCTCTAGCTCAATATGCGAAATCAAATGGTTCTCTTAGCAAGAAGGCGATGCTGGCAATTTCACTAGGATCTGTTACAGTTTTCCTTCTCTTACTTACCCTTTATTGGTTGGTAAGCATGAAGATGAAAGGCAAGCGTAGACGAAATAAATGTTCATTTATACTTACGGCCGGGTCAACCTACTTCGAAGAATCTTCTGGTGGAACAACTTTTGATGATAGTAGAATAAACTCAGAGTTAGCATTCTTTCATCTAAAAACCATAACAACCACCACAAACAATTTCTCCATTGAAAACAAGCTTGGAGAAGGAGGTTTTGGCTCCGTTTATAAGGGCATACTTCACAATGGAAAGGAAATAGCAGTAAAAAGACTATCCAAGTTTTCTGGCCAAGGAGTTGAAGAGTTTAAGAATGAAGTTCTTCTGATTGCAAAACTCCAACACAGAAACCTTGTCAGGATTTTAGGTTGTTGTGTTGAAGATGAAGAGAAGATTCTAATCTATGAATACTTGCCAAACAAAAGTTTGGACTCTTTCATCTTCAATGAAACAAAGAGAGCACTTTTAAATTGGACAAAACGTTTCGAGATTATCTTTGGGATTGCTAGAGGGGTATTATATCTTCATGAAGATTCAAGATTAAGAATTATCCATAGAGATCTAAAGGCCAGCAATGTCCTATTGGATAATTCTTTGAACCCAAAAATTGCAGATTTTGGTATGGCTAGAATATTTAGTGGGGATCAAAGTGAAGCGAATACCAATCGTGTGGTTGGAACATATGGTTATATGTCCCCGGAATATGCAATGGAAGGACTGTTTTCAATAAAGTCTGATGTATATAGTTTTGGTGTTTTACTGCTAGAAATCATCACTGGCAAAAAGAATGTTGGCTATTACCATGAGGAGTATCCTAATTCAAATTTGGTTGGACATGTTTGGAATTTGTGGAGGGAAGATTATGCCTTGGAAATAGTTGATTCATCTCTGGGAGACTCGTTGCAGTTTGATGAAGTTCTGAGATGTATACAAATTGCGCTCTTGTGTGTGCAAGATTTTCCCACCGAGCGACCAACCATGTCAGCAGTTCTTGTAATGTTAAGTAATGATGCAGCTCTTCCTCCGCCGGGAAAACCTGCATTTCTAGTGAAGAAAGCTGGTACCAGTGAAGAAAATTCTATCAATTATGTGACATGTACTATTGTAAGAGCTCGGTAG

Protein Analysis

834

Amino Acids

92.94

Weight (kDa)

6.5

Isoelectric Point (pI)

43.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 75 - 179 1.1e-33 D-mannose binding lectin
S_locus_glycop PF00954 211 - 323 1.2e-24 S-locus glycoprotein domain
PAN_2 PF08276 345 - 412 2.2e-19 PAN-like domain
PK_Tyr_Ser-Thr PF07714 525 - 792 4.3e-48 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 525 - 792 6.2e-44 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1617
AasI GACNNNNNNGTC 2 cut(s) 125, 891
Acc36I ACCTGC 1 cut(s) 2431
Acc65I GGTACC 2 cut(s) 1075, 2447
AccB1I GGYRCC 2 cut(s) 1075, 2447
AccB7I CCANNNNNTGG 1 cut(s) 231
AccBSI CCGCTC 3 cut(s) 639, 666, 1186
AccI GTMKAC 2 cut(s) 1426, 2314
AciI CCGC 8 cut(s) 184, 342, 502, 634, 639, 666, 1186, 2411
AclI AACGTT 2 cut(s) 348, 1867
AclWI GGATC 4 cut(s) 656, 848, 1363, 2037
AcoI YGGCCR 2 cut(s) 1455, 1675
AcsI RAATTY 3 cut(s) 2209, 2230, 2461
AcuI CTGAAG 1 cut(s) 145
AfaI GTAC 6 cut(s) 328, 568, 1077, 1098, 2449, 2485
AfiI CCNNNNNNNGG 8 cut(s) 231, 336, 387, 557, 650, 1253, 1392, 2414
AflIII ACRYGT 3 cut(s) 1099, 2221, 2480
AgsI TTSAA 9 cut(s) 703, 1580, 1688, 1766, 1831, 1923, 1984, 2112, 2208
AjuI GAANNNNNNNTTGG 4 cut(s) 1572, 1587, 1604, 1619
AloI GAACNNNNNNTCC 2 cut(s) 488, 520
Alw21I GWGCWC 2 cut(s) 1849, 2500
Alw26I GTCTC 5 cut(s) 105, 133, 597, 856, 2274
AlwI GGATC 4 cut(s) 656, 848, 1363, 2037
Ama87I CYCGRG 2 cut(s) 550, 583
AoxI GGCC 6 cut(s) 298, 622, 640, 1455, 1675, 1953
ApeKI GCWGC 2 cut(s) 17, 2399
ApoI RAATTY 3 cut(s) 2209, 2230, 2461
ArsI GACNNNNNNTTYG 2 cut(s) 914, 946
Asp700I GAANNNNTTC 1 cut(s) 1707
Asp718I GGTACC 2 cut(s) 1075, 2447
AspLEI GCGC 2 cut(s) 17, 2326
AspS9I GGNCC 6 cut(s) 319, 339, 504, 623, 641, 893
AsuC2I CCSGG 9 cut(s) 168, 508, 551, 552, 559, 951, 1459, 2084, 2415
AsuHPI GGTGA 2 cut(s) 302, 680
AsuII TTCGAA 2 cut(s) 957, 1473
AvaI CYCGRG 2 cut(s) 550, 583
AvaII GGWCC 4 cut(s) 319, 339, 504, 893
BaeI ACNNNNGTAYC 2 cut(s) 310, 343
BalI TGGCCA 1 cut(s) 1677
BanI GGYRCC 2 cut(s) 1075, 2447
BanII GRGCYC 1 cut(s) 2500
BarI GAAGNNNNNNTAC 2 cut(s) 1893, 1925
BauI CACGAG 3 cut(s) 972, 1171, 1234
BbsI GAAGAC 1 cut(s) 732
Bbv12I GWGCWC 2 cut(s) 1849, 2500
BbvCI CCTCAGC 1 cut(s) 177
BbvI GCAGC 2 cut(s) 29, 2411
BccI CCATC 5 cut(s) 113, 287, 654, 1141, 1222
BceAI ACGGC 5 cut(s) 130, 824, 1107, 1179, 1470
BciVI GTATCC 2 cut(s) 385, 2208
BcnI CCSGG 9 cut(s) 168, 508, 551, 552, 559, 951, 1459, 2084, 2415
BcoDI GTCTC 5 cut(s) 105, 133, 597, 856, 2274
BfuAI ACCTGC 1 cut(s) 2431
BfuI GTATCC 2 cut(s) 385, 2208
BglI GCCNNNNNGGC 1 cut(s) 951
BglII AGATCT 1 cut(s) 1945
BisI GCNGC 3 cut(s) 18, 640, 2400
BlsI GCNGC 3 cut(s) 19, 641, 2401
Bme1390I CCNGG 9 cut(s) 168, 508, 551, 552, 559, 951, 1459, 2084, 2415
Bme18I GGWCC 4 cut(s) 319, 339, 504, 893
BmeT110I CYCGRG 2 cut(s) 550, 583
BmgT120I GGNCC 6 cut(s) 319, 339, 504, 623, 641, 893
BmiI GGNNCC 5 cut(s) 521, 556, 1077, 1609, 2449
BmrFI CCNGG 9 cut(s) 168, 508, 551, 552, 559, 951, 1459, 2084, 2415
BmrI ACTGGG 1 cut(s) 248
BmsI GCATC 4 cut(s) 72, 1270, 1326, 2386
BmuI ACTGGG 1 cut(s) 248
BpiI GAAGAC 1 cut(s) 732
BplI GAGNNNNNCTC 2 cut(s) 577, 609
BpmI CTGGAG 1 cut(s) 291
Bpu10I CCTNAGC 1 cut(s) 177
Bpu14I TTCGAA 2 cut(s) 957, 1473
BpuEI CTTGAG 1 cut(s) 434
BpuMI CCSGG 9 cut(s) 168, 508, 551, 552, 559, 951, 1459, 2084, 2415
Bsa29I ATCGAT 1 cut(s) 281
BsaAI YACGTR 1 cut(s) 1100
BsaBI GATNNNNATC 1 cut(s) 1784
BsaI GGTCTC 2 cut(s) 597, 856
BsaJI CCNNGG 7 cut(s) 166, 550, 584, 644, 1678, 2082, 2253
BsaWI WCCGGW 1 cut(s) 1078
BsaXI ACNNNNNCTCC 6 cut(s) 288, 318, 1014, 1044, 1556, 1586
Bsc4I CCNNNNNNNGG 8 cut(s) 231, 336, 387, 557, 650, 1253, 1392, 2414
Bse118I RCCGGY 1 cut(s) 498
Bse1I ACTGG 4 cut(s) 254, 983, 2167, 2451
Bse3DI GCAATG 2 cut(s) 1966, 2100
Bse8I GATNNNNATC 1 cut(s) 1784
BseCI ATCGAT 1 cut(s) 281
BseDI CCNNGG 7 cut(s) 166, 550, 584, 644, 1678, 2082, 2253
BseJI GATNNNNATC 1 cut(s) 1784
BseLI CCNNNNNNNGG 8 cut(s) 231, 336, 387, 557, 650, 1253, 1392, 2414
BseMI GCAATG 2 cut(s) 1966, 2100
BseMII CTCAG 4 cut(s) 191, 474, 1533, 2297
BseNI ACTGG 4 cut(s) 254, 983, 2167, 2451
BseRI GAGGAG 1 cut(s) 2207
BseX3I CGGCCG 1 cut(s) 1455
BseXI GCAGC 2 cut(s) 29, 2411
BsgI GTGCAG 1 cut(s) 1197
Bsh1285I CGRYCG 1 cut(s) 1458
BshFI GGCC 6 cut(s) 300, 624, 642, 1457, 1677, 1955
BshNI GGYRCC 2 cut(s) 1075, 2447
BshVI ATCGAT 1 cut(s) 281
BsiEI CGRYCG 1 cut(s) 1458
BsiHKAI GWGCWC 2 cut(s) 1849, 2500
BsiHKCI CYCGRG 2 cut(s) 550, 583
BslFI GGGAC 3 cut(s) 668, 1235, 2065
BslI CCNNNNNNNGG 8 cut(s) 231, 336, 387, 557, 650, 1253, 1392, 2414
BsmAI GTCTC 5 cut(s) 105, 133, 597, 856, 2274
BsmBI CGTCTC 1 cut(s) 105
BsmFI GGGAC 3 cut(s) 668, 1235, 2065
BsmI GAATGC 1 cut(s) 1529
BsnI GGCC 6 cut(s) 300, 624, 642, 1457, 1677, 1955
Bso31I GGTCTC 2 cut(s) 597, 856
BsoBI CYCGRG 2 cut(s) 550, 583
Bsp119I TTCGAA 2 cut(s) 957, 1473
Bsp1286I GDGCHC 2 cut(s) 1849, 2500
Bsp1407I TGTACA 1 cut(s) 566
Bsp143I GATC 6 cut(s) 282, 648, 840, 1355, 1945, 2029
BspACI CCGC 8 cut(s) 184, 342, 502, 634, 639, 666, 1186, 2411
BspANI GGCC 6 cut(s) 300, 624, 642, 1457, 1677, 1955
BspCNI CTCAG 4 cut(s) 190, 473, 1532, 2298
BspDI ATCGAT 1 cut(s) 281
BspHI TCATGA 1 cut(s) 1912
BspLI GGNNCC 5 cut(s) 521, 556, 1077, 1609, 2449
BspMI ACCTGC 1 cut(s) 2431
BspPI GGATC 4 cut(s) 656, 848, 1363, 2037
BspQI GCTCTTC 1 cut(s) 2409
BspT104I TTCGAA 2 cut(s) 957, 1473
BspT107I GGYRCC 2 cut(s) 1075, 2447
BspTNI GGTCTC 2 cut(s) 597, 856
BsrBI CCGCTC 3 cut(s) 639, 666, 1186
BsrDI GCAATG 2 cut(s) 1966, 2100
BsrFI RCCGGY 1 cut(s) 498
BsrGI TGTACA 1 cut(s) 566
BsrI ACTGG 4 cut(s) 254, 983, 2167, 2451
BssAI RCCGGY 1 cut(s) 498
BssECI CCNNGG 7 cut(s) 166, 550, 584, 644, 1678, 2082, 2253
BssMI GATC 6 cut(s) 282, 648, 840, 1355, 1945, 2029
BssNAI GTATAC 1 cut(s) 2315
BssSI CACGAG 3 cut(s) 972, 1171, 1234
BssT1I CCWWGG 3 cut(s) 644, 1678, 2253
Bst1107I GTATAC 1 cut(s) 2315
Bst2BI CACGAG 3 cut(s) 972, 1171, 1234
Bst4CI ACNGT 6 cut(s) 469, 600, 691, 908, 1366, 2106
Bst6I CTCTTC 5 cut(s) 364, 704, 1683, 1767, 2409
BstAUI TGTACA 1 cut(s) 566
BstBAI YACGTR 1 cut(s) 1100
BstBI TTCGAA 2 cut(s) 957, 1473
BstC8I GCNNGC 4 cut(s) 937, 1341, 1421, 1957
BstDEI CTNAG 5 cut(s) 177, 460, 1322, 1519, 2306
BstHHI GCGC 2 cut(s) 17, 2326
BstKTI GATC 6 cut(s) 285, 651, 843, 1358, 1948, 2032
BstMAI GTCTC 5 cut(s) 105, 133, 597, 856, 2274
BstMBI GATC 6 cut(s) 282, 648, 840, 1355, 1945, 2029
BstMCI CGRYCG 1 cut(s) 1458
BstMWI GCNNNNNNNGC 4 cut(s) 499, 951, 1301, 1340
BstNSI RCATGY 2 cut(s) 2225, 2484
BstSCI CCNGG 9 cut(s) 166, 506, 549, 550, 557, 949, 1457, 2082, 2413
BstV1I GCAGC 2 cut(s) 29, 2411
BstV2I GAAGAC 1 cut(s) 732
BstX2I RGATCY 2 cut(s) 1355, 1945
BstXI CCANNNNNNTGG 1 cut(s) 2056
BstYI RGATCY 2 cut(s) 1355, 1945
BstZ17I GTATAC 1 cut(s) 2315
BstZI CGGCCG 1 cut(s) 1455
Bsu15I ATCGAT 1 cut(s) 281
BsuI GTATCC 2 cut(s) 385, 2208
BsuRI GGCC 6 cut(s) 300, 624, 642, 1457, 1677, 1955
BsuTUI ATCGAT 1 cut(s) 281
BtgZI GCGATG 1 cut(s) 1349
BtsIMutI CAGTG 4 cut(s) 292, 474, 2160, 2458
BveI ACCTGC 1 cut(s) 2431
Cac8I GCNNGC 4 cut(s) 937, 1341, 1421, 1957
CciI TCATGA 1 cut(s) 1912
CfoI GCGC 2 cut(s) 17, 2326
Cfr10I RCCGGY 1 cut(s) 498
Cfr13I GGNCC 6 cut(s) 319, 339, 504, 623, 641, 893
Cfr9I CCCGGG 1 cut(s) 550
ClaI ATCGAT 1 cut(s) 281
CpoI CGGWCCG 1 cut(s) 504
Csp6I GTAC 6 cut(s) 327, 567, 1076, 1097, 2448, 2484
CspCI CAANNNNNGTGG 2 cut(s) 673, 708
CspI CGGWCCG 1 cut(s) 504
CviAII CATG 8 cut(s) 814, 1212, 1405, 1913, 2189, 2222, 2365, 2481
CviQI GTAC 6 cut(s) 327, 567, 1076, 1097, 2448, 2484
DdeI CTNAG 5 cut(s) 177, 460, 1322, 1519, 2306
DpnI GATC 6 cut(s) 284, 650, 842, 1357, 1947, 2031
DpnII GATC 6 cut(s) 282, 648, 840, 1355, 1945, 2029
DraI TTTAAA 1 cut(s) 1854
DrdI GACNNNNNNGTC 2 cut(s) 125, 891
DseDI GACNNNNNNGTC 2 cut(s) 125, 891
EaeI YGGCCR 2 cut(s) 1455, 1675
EagI CGGCCG 1 cut(s) 1455
Eam1104I CTCTTC 5 cut(s) 364, 704, 1683, 1767, 2409
EarI CTCTTC 5 cut(s) 364, 704, 1683, 1767, 2409
EciI GGCGGA 2 cut(s) 649, 2400
Ecl136II GAGCTC 1 cut(s) 2498
EclXI CGGCCG 1 cut(s) 1455
Eco130I CCWWGG 3 cut(s) 644, 1678, 2253
Eco147I AGGCCT 1 cut(s) 300
Eco24I GRGCYC 1 cut(s) 2500
Eco31I GGTCTC 2 cut(s) 597, 856
Eco32I GATATC 1 cut(s) 735
Eco47I GGWCC 4 cut(s) 319, 339, 504, 893
Eco52I CGGCCG 1 cut(s) 1455
Eco53kI GAGCTC 1 cut(s) 2498
Eco57I CTGAAG 1 cut(s) 145
Eco88I CYCGRG 2 cut(s) 550, 583
EcoICRI GAGCTC 1 cut(s) 2498
EcoO109I RGGNCCY 1 cut(s) 623
EcoRV GATATC 1 cut(s) 735
EcoT14I CCWWGG 3 cut(s) 644, 1678, 2253
EcoT38I GRGCYC 1 cut(s) 2500
ErhI CCWWGG 3 cut(s) 644, 1678, 2253
Esp3I CGTCTC 1 cut(s) 105
FaeI CATG 8 cut(s) 817, 1215, 1408, 1916, 2192, 2225, 2368, 2484
FaqI GGGAC 3 cut(s) 668, 1235, 2065
FatI CATG 8 cut(s) 813, 1211, 1404, 1912, 2188, 2221, 2364, 2480
FauI CCCGC 1 cut(s) 1179
FauNDI CATATG 2 cut(s) 571, 2068
FblI GTMKAC 2 cut(s) 1426, 2314
Fnu4HI GCNGC 3 cut(s) 18, 640, 2400
FriOI GRGCYC 1 cut(s) 2500
Fsp4HI GCNGC 3 cut(s) 18, 640, 2400
GlaI GCGC 2 cut(s) 16, 2325
GluI GCNGC 3 cut(s) 18, 640, 2400
GsuI CTGGAG 1 cut(s) 291
HaeIII GGCC 6 cut(s) 300, 624, 642, 1457, 1677, 1955
HhaI GCGC 2 cut(s) 17, 2326
Hin1II CATG 8 cut(s) 817, 1215, 1408, 1916, 2192, 2225, 2368, 2484
Hin6I GCGC 2 cut(s) 15, 2324
HinP1I GCGC 2 cut(s) 15, 2324
HincII GTYRAC 2 cut(s) 718, 1464
HindII GTYRAC 2 cut(s) 718, 1464
HindIII AAGCTT 4 cut(s) 383, 485, 1125, 1586
HphI GGTGA 2 cut(s) 302, 680
Hpy166II GTNNAC 6 cut(s) 671, 718, 771, 1427, 1464, 2315
Hpy188I TCNGA 9 cut(s) 110, 463, 609, 856, 1038, 1522, 1713, 2122, 2307
Hpy8I GTNNAC 6 cut(s) 671, 718, 771, 1427, 1464, 2315
Hpy99I CGWCG 1 cut(s) 122
HpyAV CCTTC 6 cut(s) 59, 1040, 1324, 1382, 1589, 2093
HpyCH4III ACNGT 6 cut(s) 469, 600, 691, 908, 1366, 2106
HpyCH4IV ACGT 6 cut(s) 120, 189, 348, 527, 1099, 1867
HpyF10VI GCNNNNNNNGC 4 cut(s) 499, 951, 1301, 1340
HpyF3I CTNAG 5 cut(s) 177, 460, 1322, 1519, 2306
HpySE526I ACGT 6 cut(s) 120, 189, 348, 527, 1099, 1867
Hsp92II CATG 8 cut(s) 817, 1215, 1408, 1916, 2192, 2225, 2368, 2484
HspAI GCGC 2 cut(s) 15, 2324
KpnI GGTACC 2 cut(s) 1079, 2451
Kzo9I GATC 6 cut(s) 282, 648, 840, 1355, 1945, 2029
LguI GCTCTTC 1 cut(s) 2409
LmnI GCTCC 5 cut(s) 525, 561, 636, 663, 1613
Lsp1109I GCAGC 2 cut(s) 29, 2411
LweI GCATC 4 cut(s) 72, 1270, 1326, 2386
MaeII ACGT 6 cut(s) 120, 189, 348, 527, 1099, 1867
MaeIII GTNAC 6 cut(s) 349, 902, 908, 974, 1360, 2476
MalI GATC 6 cut(s) 284, 650, 842, 1357, 1947, 2031
MbiI CCGCTC 3 cut(s) 639, 666, 1186
MboI GATC 6 cut(s) 282, 648, 840, 1355, 1945, 2029
MflI RGATCY 2 cut(s) 1355, 1945
MhlI GDGCHC 2 cut(s) 1849, 2500
MlsI TGGCCA 1 cut(s) 1677
MluNI TGGCCA 1 cut(s) 1677
MlyI GAGTC 6 cut(s) 690, 755, 879, 1076, 1811, 2276
MmeI TCCRAC 8 cut(s) 172, 492, 673, 879, 1016, 1750, 2041, 2197
Mox20I TGGCCA 1 cut(s) 1677
MroXI GAANNNNTTC 1 cut(s) 1707
MscI TGGCCA 1 cut(s) 1677
MseI TTAA 5 cut(s) 707, 1695, 1853, 1928, 2387
MslI CAYNNNNRTG 1 cut(s) 1409
Msp20I TGGCCA 1 cut(s) 1677
MspR9I CCNGG 9 cut(s) 168, 508, 551, 552, 559, 951, 1459, 2084, 2415
Mva1269I GAATGC 1 cut(s) 1529
MwoI GCNNNNNNNGC 4 cut(s) 499, 951, 1301, 1340
NciI CCSGG 9 cut(s) 168, 508, 551, 552, 559, 951, 1459, 2084, 2415
NdeI CATATG 2 cut(s) 571, 2068
NdeII GATC 6 cut(s) 282, 648, 840, 1355, 1945, 2029
NlaIII CATG 8 cut(s) 817, 1215, 1408, 1916, 2192, 2225, 2368, 2484
NlaIV GGNNCC 5 cut(s) 521, 556, 1077, 1609, 2449
NmuCI GTSAC 3 cut(s) 908, 974, 2476
NspI RCATGY 2 cut(s) 2225, 2484
NspV TTCGAA 2 cut(s) 957, 1473
PagI TCATGA 1 cut(s) 1912
PceI AGGCCT 1 cut(s) 300
PciI ACATGT 2 cut(s) 2221, 2480
PciSI GCTCTTC 1 cut(s) 2409
PcsI WCGNNNNNNNCGW 1 cut(s) 1169
PctI GAATGC 1 cut(s) 1529
PdmI GAANNNNTTC 1 cut(s) 1707
PfeI GAWTC 6 cut(s) 263, 791, 1478, 1780, 1919, 2267
PflMI CCANNNNNTGG 1 cut(s) 231
PkrI GCNGC 3 cut(s) 19, 641, 2401
PleI GAGTC 6 cut(s) 690, 755, 879, 1076, 1811, 2276
PpsI GAGTC 6 cut(s) 690, 755, 879, 1076, 1811, 2276
Ppu21I YACGTR 1 cut(s) 1100
PscI ACATGT 2 cut(s) 2221, 2480
PsiI TTATAA 1 cut(s) 1617
Psp124BI GAGCTC 1 cut(s) 2500
Psp1406I AACGTT 2 cut(s) 348, 1867
PspN4I GGNNCC 5 cut(s) 521, 556, 1077, 1609, 2449
PspPI GGNCC 6 cut(s) 319, 339, 504, 623, 641, 893
PsuI RGATCY 2 cut(s) 1355, 1945
RsaI GTAC 6 cut(s) 328, 568, 1077, 1098, 2449, 2485
RsaNI GTAC 6 cut(s) 327, 567, 1076, 1097, 2448, 2484
RseI CAYNNNNRTG 1 cut(s) 1409
Rsr2I CGGWCCG 1 cut(s) 504
RsrII CGGWCCG 1 cut(s) 504
SacI GAGCTC 1 cut(s) 2500
SapI GCTCTTC 1 cut(s) 2409
SaqAI TTAA 5 cut(s) 707, 1695, 1853, 1928, 2387
SatI GCNGC 3 cut(s) 18, 640, 2400
Sau3AI GATC 6 cut(s) 282, 648, 840, 1355, 1945, 2029
Sau96I GGNCC 6 cut(s) 319, 339, 504, 623, 641, 893
SchI GAGTC 6 cut(s) 690, 755, 879, 1076, 1811, 2276
ScrFI CCNGG 9 cut(s) 168, 508, 551, 552, 559, 951, 1459, 2084, 2415
SduI GDGCHC 2 cut(s) 1849, 2500
SfaNI GCATC 4 cut(s) 72, 1270, 1326, 2386
SfuI TTCGAA 2 cut(s) 957, 1473
SinI GGWCC 4 cut(s) 319, 339, 504, 893
SmaI CCCGGG 1 cut(s) 552
SmiMI CAYNNNNRTG 1 cut(s) 1409
SmlI CTYRAG 1 cut(s) 413
SmoI CTYRAG 1 cut(s) 413
SseBI AGGCCT 1 cut(s) 300
SsiI CCGC 8 cut(s) 184, 342, 502, 634, 639, 666, 1186, 2411
SspI AATATT 1 cut(s) 2019
SstI GAGCTC 1 cut(s) 2500
StuI AGGCCT 1 cut(s) 300
StyD4I CCNGG 9 cut(s) 166, 506, 549, 550, 557, 949, 1457, 2082, 2413
StyI CCWWGG 3 cut(s) 644, 1678, 2253
TaaI ACNGT 6 cut(s) 469, 600, 691, 908, 1366, 2106
TaiI ACGT 6 cut(s) 123, 192, 351, 530, 1102, 1870
TaqI TCGA 6 cut(s) 281, 843, 957, 1273, 1473, 1872
TatI WGTACW 2 cut(s) 566, 2483
TauI GCSGC 1 cut(s) 642
TfiI GAWTC 6 cut(s) 263, 791, 1478, 1780, 1919, 2267
Tru1I TTAA 5 cut(s) 707, 1695, 1853, 1928, 2387
Tru9I TTAA 5 cut(s) 707, 1695, 1853, 1928, 2387
TscAI CASTG 4 cut(s) 292, 474, 2167, 2458
TseFI GTSAC 3 cut(s) 908, 974, 2476
TseI GCWGC 2 cut(s) 17, 2399
Tsp45I GTSAC 3 cut(s) 908, 974, 2476
TspGWI ACGGA 3 cut(s) 327, 1056, 1600
TspMI CCCGGG 1 cut(s) 550
TspRI CASTG 4 cut(s) 292, 474, 2167, 2458
Van91I CCANNNNNTGG 1 cut(s) 231
VpaK11BI GGWCC 4 cut(s) 319, 339, 504, 893
XapI RAATTY 3 cut(s) 2209, 2230, 2461
XceI RCATGY 2 cut(s) 2225, 2484
XmaI CCCGGG 1 cut(s) 550
XmiI GTMKAC 2 cut(s) 1426, 2314
XmnI GAANNNNTTC 1 cut(s) 1707
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.