Rorug05G0063300

Plant protein of unknown function (DUF936)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0008168
Physical Location & Seq
Forward (+)
5516965 .. 5519252
2288 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0063300.1

Sequence Viewer

Length: 822 bp
ATGGCAGCCAGCAATGTCATCTTCAACATCTCGAGGTCGCCGTTAACTCAATACCCGGCTTCACACTCTCTCAGTCCCTCTCTCAAAACCTCCAGGGTTTGCTTCACCTCCGCCTCCAAATTCTCCAAGGGTCGAAATGCAGCGGAAAAGAATAAGGACTACTCAAGTTCCCGCCGGGCGTATGACGGAAAGGACTGGAGTACCAAAGCAAAGCCAACCAGGGAAGAAGATGTTGAAGATGACAAGCCACTCTTAGAGAAAATTAAGGGGGCAGCGACGGAGTACGCGGAAGAAACGATGGAGAACGTAAAAGGCGCATCGGAGACTATATCAGAGACGACCAAGGAGTATGCAGATGATGCAAAGGAGAAGACAAACGAGGCAGCGGAGACTATAACGGCCAAGGCGGAGGAGGGGACTAACAAGGTGGCGGAGCCGGAGACGACGGAGAGTGACAGTGAGAATGTCAAGGAGAGTTACAGTGAGAAGAGACTCGTAAGTATCACTCTTCTCAATCTCTCTGCAATTTCGCTTGTTAGGATTGTGAATTATTCCAGATCCATGAGTACCATAGGAGACATCCAGCTGCACGCACTAGTTGTTGATGCCAATGAGGAACATGAAGCACGAGCCTCAAGTGGAGAGACGATTGATAGCAACAACTATCCTGAGACTGTGAATTGTATGTTTATCATTAATGCTGGTTCTGGTTTTAGAATGTTGTGGAGCACTGTGAAGTCCTTCCTTGATCCCAAGACAACTGAAAAGATTAATGTCCTTGGTGACAAGAGCAAGTTACTTCAAATAATTGATGCTAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF936

Protein Analysis

273

Amino Acids

30.09

Weight (kDa)

5.2

Isoelectric Point (pI)

45.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO PF00650 218 - 271 1.3e-11 CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 287
AciI CCGC 7 cut(s) 111, 143, 172, 287, 386, 407, 431
AclWI GGATC 2 cut(s) 552, 743
AcoI YGGCCR 1 cut(s) 399
AcsI RAATTY 1 cut(s) 119
AfaI GTAC 3 cut(s) 202, 284, 568
AgsI TTSAA 3 cut(s) 25, 236, 803
AhlI ACTAGT 1 cut(s) 595
AjnI CCWGG 2 cut(s) 92, 218
AluBI AGCT 1 cut(s) 586
AluI AGCT 1 cut(s) 586
Alw21I GWGCWC 1 cut(s) 731
Alw26I GTCTC 8 cut(s) 317, 329, 383, 434, 484, 570, 638, 665
AlwI GGATC 2 cut(s) 552, 743
Ama87I CYCGRG 1 cut(s) 31
AoxI GGCC 1 cut(s) 399
ApeKI GCWGC 5 cut(s) 5, 140, 272, 383, 586
ApoI RAATTY 1 cut(s) 119
AseI ATTAAT 2 cut(s) 696, 771
Asp700I GAANNNNTTC 1 cut(s) 740
AspLEI GCGC 1 cut(s) 317
AsuC2I CCSGG 2 cut(s) 56, 176
AsuHPI GGTGA 2 cut(s) 97, 794
AvaI CYCGRG 1 cut(s) 31
BaeI ACNNNNGTAYC 2 cut(s) 184, 217
BauI CACGAG 1 cut(s) 627
BbsI GAAGAC 1 cut(s) 377
Bbv12I GWGCWC 1 cut(s) 731
BbvI GCAGC 5 cut(s) 17, 152, 284, 395, 573
BccI CCATC 1 cut(s) 292
BceAI ACGGC 2 cut(s) 25, 414
BciT130I CCWGG 2 cut(s) 94, 220
BcnI CCSGG 2 cut(s) 56, 176
BcoDI GTCTC 8 cut(s) 317, 329, 383, 434, 484, 570, 638, 665
BcuI ACTAGT 1 cut(s) 595
BfaI CTAG 2 cut(s) 596, 816
BisI GCNGC 5 cut(s) 6, 141, 273, 384, 587
BlsI GCNGC 5 cut(s) 7, 142, 274, 385, 588
Bme1390I CCNGG 4 cut(s) 56, 94, 176, 220
BmeT110I CYCGRG 1 cut(s) 31
BmiI GGNNCC 1 cut(s) 435
BmrFI CCNGG 4 cut(s) 56, 94, 176, 220
BmsI GCATC 4 cut(s) 326, 349, 595, 802
BpiI GAAGAC 1 cut(s) 377
BpmI CTGGAG 2 cut(s) 76, 217
BpuEI CTTGAG 2 cut(s) 148, 619
BpuMI CCSGG 2 cut(s) 56, 176
BsaJI CCNNGG 6 cut(s) 93, 126, 219, 342, 402, 778
Bse1I ACTGG 1 cut(s) 200
Bse3DI GCAATG 1 cut(s) 19
BseBI CCWGG 2 cut(s) 94, 220
BseDI CCNNGG 6 cut(s) 93, 126, 219, 342, 402, 778
BseGI GGATG 1 cut(s) 579
BseMI GCAATG 1 cut(s) 19
BseMII CTCAG 2 cut(s) 85, 660
BseNI ACTGG 1 cut(s) 200
BseRI GAGGAG 1 cut(s) 425
BseXI GCAGC 5 cut(s) 17, 152, 284, 395, 573
BsgI GTGCAG 1 cut(s) 572
Bsh1236I CGCG 1 cut(s) 287
BshFI GGCC 1 cut(s) 401
BsiHKAI GWGCWC 1 cut(s) 731
BsiHKCI CYCGRG 1 cut(s) 31
BsiSI CCGG 3 cut(s) 56, 175, 437
BslFI GGGAC 2 cut(s) 60, 430
BsmAI GTCTC 8 cut(s) 317, 329, 383, 434, 484, 570, 638, 665
BsmBI CGTCTC 3 cut(s) 329, 434, 638
BsmFI GGGAC 2 cut(s) 60, 430
BsnI GGCC 1 cut(s) 401
BsoBI CYCGRG 1 cut(s) 31
Bsp1286I GDGCHC 1 cut(s) 731
Bsp143I GATC 2 cut(s) 557, 748
BspACI CCGC 7 cut(s) 111, 143, 172, 287, 386, 407, 431
BspANI GGCC 1 cut(s) 401
BspCNI CTCAG 2 cut(s) 84, 661
BspFNI CGCG 1 cut(s) 287
BspLI GGNNCC 1 cut(s) 435
BspPI GGATC 2 cut(s) 552, 743
BsrDI GCAATG 1 cut(s) 19
BsrI ACTGG 1 cut(s) 200
BssECI CCNNGG 6 cut(s) 93, 126, 219, 342, 402, 778
BssMI GATC 2 cut(s) 557, 748
BssSI CACGAG 1 cut(s) 627
BssT1I CCWWGG 4 cut(s) 126, 342, 402, 778
Bst2BI CACGAG 1 cut(s) 627
Bst2UI CCWGG 2 cut(s) 94, 220
Bst4CI ACNGT 4 cut(s) 458, 482, 676, 733
Bst6I CTCTTC 2 cut(s) 482, 513
BstAPI GCANNNNNTGC 1 cut(s) 359
BstC8I GCNNGC 2 cut(s) 10, 591
BstDEI CTNAG 3 cut(s) 71, 253, 669
BstF5I GGATG 1 cut(s) 579
BstFNI CGCG 1 cut(s) 287
BstHHI GCGC 1 cut(s) 317
BstKTI GATC 2 cut(s) 560, 751
BstMAI GTCTC 8 cut(s) 317, 329, 383, 434, 484, 570, 638, 665
BstMBI GATC 2 cut(s) 557, 748
BstMWI GCNNNNNNNGC 1 cut(s) 359
BstNI CCWGG 2 cut(s) 94, 220
BstSCI CCNGG 4 cut(s) 54, 92, 174, 218
BstUI CGCG 1 cut(s) 287
BstV1I GCAGC 5 cut(s) 17, 152, 284, 395, 573
BstV2I GAAGAC 1 cut(s) 377
BstX2I RGATCY 1 cut(s) 557
BstYI RGATCY 1 cut(s) 557
BsuRI GGCC 1 cut(s) 401
BtsCI GGATG 1 cut(s) 579
BtsIMutI CAGTG 3 cut(s) 463, 487, 729
Cac8I GCNNGC 2 cut(s) 10, 591
CfoI GCGC 1 cut(s) 317
Csp6I GTAC 3 cut(s) 201, 283, 567
CviAII CATG 2 cut(s) 562, 620
CviJI RGCY 8 cut(s) 8, 59, 214, 247, 401, 436, 586, 632
CviKI_1 RGCY 8 cut(s) 8, 59, 214, 247, 401, 436, 586, 632
CviQI GTAC 3 cut(s) 201, 283, 567
DdeI CTNAG 3 cut(s) 71, 253, 669
DpnI GATC 2 cut(s) 559, 750
DpnII GATC 2 cut(s) 557, 748
EaeI YGGCCR 1 cut(s) 399
Eam1104I CTCTTC 2 cut(s) 482, 513
EarI CTCTTC 2 cut(s) 482, 513
EciI GGCGGA 3 cut(s) 100, 422, 446
Eco130I CCWWGG 4 cut(s) 126, 342, 402, 778
Eco88I CYCGRG 1 cut(s) 31
EcoRII CCWGG 2 cut(s) 92, 218
EcoT14I CCWWGG 4 cut(s) 126, 342, 402, 778
ErhI CCWWGG 4 cut(s) 126, 342, 402, 778
Esp3I CGTCTC 3 cut(s) 329, 434, 638
FaeI CATG 2 cut(s) 565, 623
FaiI YATR 8 cut(s) 183, 329, 351, 395, 563, 572, 621, 686
FalI AAGNNNNNCTT 2 cut(s) 236, 268
FaqI GGGAC 2 cut(s) 60, 430
FatI CATG 2 cut(s) 561, 619
FauI CCCGC 1 cut(s) 179
Fnu4HI GCNGC 5 cut(s) 6, 141, 273, 384, 587
FokI GGATG 1 cut(s) 566
Fsp4HI GCNGC 5 cut(s) 6, 141, 273, 384, 587
FspBI CTAG 2 cut(s) 596, 816
GlaI GCGC 1 cut(s) 316
GluI GCNGC 5 cut(s) 6, 141, 273, 384, 587
GsuI CTGGAG 2 cut(s) 76, 217
HaeIII GGCC 1 cut(s) 401
HapII CCGG 3 cut(s) 56, 175, 437
HhaI GCGC 1 cut(s) 317
Hin1II CATG 2 cut(s) 565, 623
Hin6I GCGC 1 cut(s) 315
HinP1I GCGC 1 cut(s) 315
HincII GTYRAC 1 cut(s) 45
HindII GTYRAC 1 cut(s) 45
HinfI GANTC 1 cut(s) 492
HpaI GTTAAC 1 cut(s) 45
HpaII CCGG 3 cut(s) 56, 175, 437
HphI GGTGA 2 cut(s) 97, 794
Hpy166II GTNNAC 1 cut(s) 45
Hpy188I TCNGA 2 cut(s) 322, 334
Hpy188III TCNNGA 3 cut(s) 31, 555, 668
Hpy8I GTNNAC 1 cut(s) 45
Hpy99I CGWCG 2 cut(s) 280, 448
HpyAV CCTTC 1 cut(s) 751
HpyCH4III ACNGT 4 cut(s) 458, 482, 676, 733
HpyCH4IV ACGT 1 cut(s) 306
HpyCH4V TGCA 5 cut(s) 140, 353, 362, 524, 589
HpyF10VI GCNNNNNNNGC 1 cut(s) 359
HpyF3I CTNAG 3 cut(s) 71, 253, 669
HpySE526I ACGT 1 cut(s) 306
Hsp92II CATG 2 cut(s) 565, 623
HspAI GCGC 1 cut(s) 315
KspAI GTTAAC 1 cut(s) 45
Kzo9I GATC 2 cut(s) 557, 748
LmnI GCTCC 2 cut(s) 433, 726
Lsp1109I GCAGC 5 cut(s) 17, 152, 284, 395, 573
LweI GCATC 4 cut(s) 326, 349, 595, 802
MaeI CTAG 2 cut(s) 596, 816
MaeII ACGT 1 cut(s) 306
MaeIII GTNAC 4 cut(s) 452, 476, 782, 795
MalI GATC 2 cut(s) 559, 750
MboI GATC 2 cut(s) 557, 748
MboII GAAGA 8 cut(s) 13, 236, 239, 248, 302, 382, 499, 500
MflI RGATCY 1 cut(s) 557
MhlI GDGCHC 1 cut(s) 731
MluCI AATT 6 cut(s) 119, 261, 525, 547, 679, 807
MlyI GAGTC 1 cut(s) 486
MroXI GAANNNNTTC 1 cut(s) 740
MseI TTAA 4 cut(s) 44, 264, 696, 771
MspA1I CMGCKG 3 cut(s) 143, 386, 586
MspI CCGG 3 cut(s) 56, 175, 437
MspR9I CCNGG 4 cut(s) 56, 94, 176, 220
MvaI CCWGG 2 cut(s) 94, 220
MvnI CGCG 1 cut(s) 287
MwoI GCNNNNNNNGC 1 cut(s) 359
NciI CCSGG 2 cut(s) 56, 176
NdeII GATC 2 cut(s) 557, 748
NlaIII CATG 2 cut(s) 565, 623
NlaIV GGNNCC 1 cut(s) 435
NmuCI GTSAC 2 cut(s) 452, 782
PaeR7I CTCGAG 1 cut(s) 31
PcsI WCGNNNNNNNCGW 1 cut(s) 38
PdmI GAANNNNTTC 1 cut(s) 740
PkrI GCNGC 5 cut(s) 7, 142, 274, 385, 588
PleI GAGTC 1 cut(s) 486
PpsI GAGTC 1 cut(s) 486
PshBI ATTAAT 2 cut(s) 696, 771
Psp6I CCWGG 2 cut(s) 92, 218
PspGI CCWGG 2 cut(s) 92, 218
PspN4I GGNNCC 1 cut(s) 435
PsuI RGATCY 1 cut(s) 557
PvuII CAGCTG 1 cut(s) 586
RsaI GTAC 3 cut(s) 202, 284, 568
RsaNI GTAC 3 cut(s) 201, 283, 567
SaqAI TTAA 4 cut(s) 44, 264, 696, 771
SatI GCNGC 5 cut(s) 6, 141, 273, 384, 587
Sau3AI GATC 2 cut(s) 557, 748
SchI GAGTC 1 cut(s) 486
ScrFI CCNGG 4 cut(s) 56, 94, 176, 220
SduI GDGCHC 1 cut(s) 731
SetI ASST 7 cut(s) 38, 92, 110, 309, 429, 588, 821
SfaNI GCATC 4 cut(s) 326, 349, 595, 802
Sfr274I CTCGAG 1 cut(s) 31
SlaI CTCGAG 1 cut(s) 31
SmlI CTYRAG 3 cut(s) 31, 163, 634
SmoI CTYRAG 3 cut(s) 31, 163, 634
SpeI ACTAGT 1 cut(s) 595
Sse9I AATT 6 cut(s) 119, 261, 525, 547, 679, 807
SsiI CCGC 7 cut(s) 111, 143, 172, 287, 386, 407, 431
SspMI CTAG 2 cut(s) 596, 816
StyD4I CCNGG 4 cut(s) 54, 92, 174, 218
StyI CCWWGG 4 cut(s) 126, 342, 402, 778
TaaI ACNGT 4 cut(s) 458, 482, 676, 733
TaiI ACGT 1 cut(s) 309
TaqI TCGA 2 cut(s) 32, 133
TasI AATT 6 cut(s) 119, 261, 525, 547, 679, 807
Tru1I TTAA 4 cut(s) 44, 264, 696, 771
Tru9I TTAA 4 cut(s) 44, 264, 696, 771
TscAI CASTG 3 cut(s) 463, 487, 736
TseFI GTSAC 2 cut(s) 452, 782
TseI GCWGC 5 cut(s) 5, 140, 272, 383, 586
Tsp45I GTSAC 2 cut(s) 452, 782
TspDTI ATGAA 1 cut(s) 636
TspGWI ACGGA 3 cut(s) 201, 293, 461
TspRI CASTG 3 cut(s) 463, 487, 736
VspI ATTAAT 2 cut(s) 696, 771
XapI RAATTY 1 cut(s) 119
XhoI CTCGAG 1 cut(s) 31
XmnI GAANNNNTTC 1 cut(s) 740
XspI CTAG 2 cut(s) 596, 816
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.