Rorug05G0100600

Interactor of constitutive active ROPs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0004195
Physical Location & Seq
Forward (+)
8872563 .. 8873227
665 bp
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UTR
Exon/CDS
Intron
Rorug05G0100600.1

Sequence Viewer

Length: 552 bp
ATGGGGAGGAGAAAAATTGAGATGAAGAAGGTGATGGACAGCAGCTCCAGGCAGGTGACTTTCTCCAAGCGCAGAACTGGACTTTTTAAGAAAGCTCATGAGATTGCTACCCTCTGTGGTGCTGAGGTGGCCATCGTTGTTTTCTCTCCCGGAGGCAAGCCCTTCTCATTTGGTAACCCAAATGTGGACTCAGTAGCAGACAGGTTTCTAAATCAGGAGGAAGAGGACAACACTGATGATCGTGGTGTTCGTCGTTCTGGAAAAGTAGTGGAGAGGCTAAACCAACAGATCGCCGATGTTGTGAAGAATTTGGAGGCTGAGAAGAAAAGGGGTGAGGTGATTGCTAAGGCACTAGTGAAAGCAGAAACGAAATGGGGGCTTCCAATCCAGGAGAGGAGCCAAGGTGAGCTTGTAAAAGTGAAGCAGTCGCTTGAGGAGTTGCGCGAGAGTGTGAAGGCAAGAGAGAGTGACGTGGAGGCATCGTCTTCTTTGCTGCTCCTGGCAAACAAGCCGGTGAAGGAGACTAAGAAGACTGTTGCTAAAAGTGGCTGA

Protein Analysis

183

Amino Acids

20.31

Weight (kDa)

9.68

Isoelectric Point (pI)

50.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 11 - 57 2.4e-25 SRF-type transcription factor (DNA-binding and dimerisation domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 43
Acc36I ACCTGC 1 cut(s) 43
AccII CGCG 1 cut(s) 444
AcoI YGGCCR 1 cut(s) 129
AcsI RAATTY 1 cut(s) 307
AfiI CCNNNNNNNGG 1 cut(s) 184
AhlI ACTAGT 1 cut(s) 352
AjiI CACGTC 1 cut(s) 472
AjnI CCWGG 3 cut(s) 47, 387, 498
AluBI AGCT 3 cut(s) 45, 95, 409
AluI AGCT 3 cut(s) 45, 95, 409
Alw26I GTCTC 1 cut(s) 515
AoxI GGCC 1 cut(s) 129
ApeKI GCWGC 2 cut(s) 42, 493
ApoI RAATTY 1 cut(s) 307
AspLEI GCGC 2 cut(s) 72, 444
AsuC2I CCSGG 1 cut(s) 150
AsuHPI GGTGA 6 cut(s) 43, 67, 344, 349, 416, 526
BalI TGGCCA 1 cut(s) 131
BbsI GAAGAC 2 cut(s) 477, 536
BbvCI CCTCAGC 1 cut(s) 123
BbvI GCAGC 2 cut(s) 54, 480
BccI CCATC 2 cut(s) 28, 140
BciT130I CCWGG 3 cut(s) 49, 389, 500
BcnI CCSGG 1 cut(s) 150
BcoDI GTCTC 1 cut(s) 515
BcuI ACTAGT 1 cut(s) 352
BfaI CTAG 1 cut(s) 353
BfuAI ACCTGC 1 cut(s) 43
BisI GCNGC 2 cut(s) 43, 494
BlsI GCNGC 2 cut(s) 44, 495
Bme1390I CCNGG 4 cut(s) 49, 150, 389, 500
BmgBI CACGTC 1 cut(s) 472
BmiI GGNNCC 1 cut(s) 398
BmrFI CCNGG 4 cut(s) 49, 150, 389, 500
BmsI GCATC 1 cut(s) 488
BpiI GAAGAC 2 cut(s) 477, 536
BpmI CTGGAG 1 cut(s) 31
Bpu10I CCTNAGC 2 cut(s) 123, 345
BpuEI CTTGAG 1 cut(s) 452
BpuMI CCSGG 1 cut(s) 150
BsaJI CCNNGG 1 cut(s) 400
BsaXI ACNNNNNCTCC 6 cut(s) 29, 59, 388, 418, 467, 497
Bsc4I CCNNNNNNNGG 1 cut(s) 184
Bse118I RCCGGY 1 cut(s) 511
Bse1I ACTGG 1 cut(s) 82
BseBI CCWGG 3 cut(s) 49, 389, 500
BseDI CCNNGG 1 cut(s) 400
BseLI CCNNNNNNNGG 1 cut(s) 184
BseMII CTCAG 3 cut(s) 114, 204, 309
BseNI ACTGG 1 cut(s) 82
BseRI GAGGAG 3 cut(s) 22, 409, 449
BseXI GCAGC 2 cut(s) 54, 480
Bsh1236I CGCG 1 cut(s) 444
BshFI GGCC 1 cut(s) 131
BsiSI CCGG 2 cut(s) 150, 512
BslI CCNNNNNNNGG 1 cut(s) 184
BsmAI GTCTC 1 cut(s) 515
BsnI GGCC 1 cut(s) 131
Bsp143I GATC 2 cut(s) 238, 288
BspANI GGCC 1 cut(s) 131
BspCNI CTCAG 3 cut(s) 115, 203, 310
BspFNI CGCG 1 cut(s) 444
BspHI TCATGA 1 cut(s) 97
BspLI GGNNCC 1 cut(s) 398
BspMI ACCTGC 1 cut(s) 43
BsrFI RCCGGY 1 cut(s) 511
BsrI ACTGG 1 cut(s) 82
BssAI RCCGGY 1 cut(s) 511
BssECI CCNNGG 1 cut(s) 400
BssMI GATC 2 cut(s) 238, 288
BssT1I CCWWGG 1 cut(s) 400
Bst2UI CCWGG 3 cut(s) 49, 389, 500
Bst4CI ACNGT 1 cut(s) 535
Bst6I CTCTTC 1 cut(s) 216
BstC8I GCNNGC 1 cut(s) 158
BstDEI CTNAG 5 cut(s) 123, 190, 318, 345, 525
BstEII GGTNACC 1 cut(s) 173
BstFNI CGCG 1 cut(s) 444
BstHHI GCGC 2 cut(s) 72, 444
BstKTI GATC 2 cut(s) 241, 291
BstMAI GTCTC 1 cut(s) 515
BstMBI GATC 2 cut(s) 238, 288
BstMWI GCNNNNNNNGC 1 cut(s) 128
BstNI CCWGG 3 cut(s) 49, 389, 500
BstPI GGTNACC 1 cut(s) 173
BstSCI CCNGG 4 cut(s) 47, 148, 387, 498
BstUI CGCG 1 cut(s) 444
BstV1I GCAGC 2 cut(s) 54, 480
BstV2I GAAGAC 2 cut(s) 477, 536
BsuRI GGCC 1 cut(s) 131
BtrI CACGTC 1 cut(s) 472
BtsIMutI CAGTG 1 cut(s) 231
BveI ACCTGC 1 cut(s) 43
Cac8I GCNNGC 1 cut(s) 158
CciI TCATGA 1 cut(s) 97
CfoI GCGC 2 cut(s) 72, 444
Cfr10I RCCGGY 1 cut(s) 511
CviAII CATG 1 cut(s) 98
DdeI CTNAG 5 cut(s) 123, 190, 318, 345, 525
DpnI GATC 2 cut(s) 240, 290
DpnII GATC 2 cut(s) 238, 288
EaeI YGGCCR 1 cut(s) 129
Eam1104I CTCTTC 1 cut(s) 216
EarI CTCTTC 1 cut(s) 216
Eco130I CCWWGG 1 cut(s) 400
Eco91I GGTNACC 1 cut(s) 173
EcoO65I GGTNACC 1 cut(s) 173
EcoRII CCWGG 3 cut(s) 47, 387, 498
EcoT14I CCWWGG 1 cut(s) 400
ErhI CCWWGG 1 cut(s) 400
FaeI CATG 1 cut(s) 101
FaiI YATR 1 cut(s) 99
FalI AAGNNNNNCTT 2 cut(s) 393, 425
FatI CATG 1 cut(s) 97
Fnu4HI GCNGC 2 cut(s) 43, 494
Fsp4HI GCNGC 2 cut(s) 43, 494
FspBI CTAG 1 cut(s) 353
GlaI GCGC 2 cut(s) 71, 443
GluI GCNGC 2 cut(s) 43, 494
GsuI CTGGAG 1 cut(s) 31
HaeIII GGCC 1 cut(s) 131
HapII CCGG 2 cut(s) 150, 512
HhaI GCGC 2 cut(s) 72, 444
Hin1II CATG 1 cut(s) 101
Hin6I GCGC 2 cut(s) 70, 442
HinP1I GCGC 2 cut(s) 70, 442
HinfI GANTC 1 cut(s) 188
HpaII CCGG 2 cut(s) 150, 512
HphI GGTGA 6 cut(s) 43, 67, 344, 349, 416, 526
Hpy166II GTNNAC 1 cut(s) 187
Hpy188III TCNNGA 3 cut(s) 98, 215, 258
Hpy8I GTNNAC 1 cut(s) 187
Hpy99I CGWCG 1 cut(s) 255
HpyAV CCTTC 4 cut(s) 22, 172, 448, 511
HpyCH4III ACNGT 1 cut(s) 535
HpyCH4IV ACGT 1 cut(s) 471
HpyF10VI GCNNNNNNNGC 1 cut(s) 128
HpyF3I CTNAG 5 cut(s) 123, 190, 318, 345, 525
HpySE526I ACGT 1 cut(s) 471
Hsp92II CATG 1 cut(s) 101
HspAI GCGC 2 cut(s) 70, 442
Kzo9I GATC 2 cut(s) 238, 288
LmnI GCTCC 3 cut(s) 50, 396, 501
Lsp1109I GCAGC 2 cut(s) 54, 480
LweI GCATC 1 cut(s) 488
MaeI CTAG 1 cut(s) 353
MaeII ACGT 1 cut(s) 471
MaeIII GTNAC 3 cut(s) 55, 173, 467
MalI GATC 2 cut(s) 240, 290
MboI GATC 2 cut(s) 238, 288
MboII GAAGA 6 cut(s) 37, 233, 316, 334, 477, 541
MlsI TGGCCA 1 cut(s) 131
MluCI AATT 2 cut(s) 15, 307
MluNI TGGCCA 1 cut(s) 131
MlyI GAGTC 1 cut(s) 182
Mox20I TGGCCA 1 cut(s) 131
MscI TGGCCA 1 cut(s) 131
MseI TTAA 1 cut(s) 87
Msp20I TGGCCA 1 cut(s) 131
MspI CCGG 2 cut(s) 150, 512
MspR9I CCNGG 4 cut(s) 49, 150, 389, 500
MvaI CCWGG 3 cut(s) 49, 389, 500
MvnI CGCG 1 cut(s) 444
MwoI GCNNNNNNNGC 1 cut(s) 128
NciI CCSGG 1 cut(s) 150
NdeII GATC 2 cut(s) 238, 288
NlaIII CATG 1 cut(s) 101
NlaIV GGNNCC 1 cut(s) 398
NmuCI GTSAC 2 cut(s) 55, 467
PagI TCATGA 1 cut(s) 97
PaqCI CACCTGC 1 cut(s) 43
PcsI WCGNNNNNNNCGW 1 cut(s) 247
PfoI TCCNGGA 2 cut(s) 148, 387
PkrI GCNGC 2 cut(s) 44, 495
PleI GAGTC 1 cut(s) 182
PpsI GAGTC 1 cut(s) 182
Psp6I CCWGG 3 cut(s) 47, 387, 498
PspEI GGTNACC 1 cut(s) 173
PspGI CCWGG 3 cut(s) 47, 387, 498
PspN4I GGNNCC 1 cut(s) 398
SaqAI TTAA 1 cut(s) 87
SatI GCNGC 2 cut(s) 43, 494
Sau3AI GATC 2 cut(s) 238, 288
SchI GAGTC 1 cut(s) 182
ScrFI CCNGG 4 cut(s) 49, 150, 389, 500
SfaNI GCATC 1 cut(s) 488
SmlI CTYRAG 1 cut(s) 431
SmoI CTYRAG 1 cut(s) 431
SpeI ACTAGT 1 cut(s) 352
Sse9I AATT 2 cut(s) 15, 307
SspMI CTAG 1 cut(s) 353
StyD4I CCNGG 4 cut(s) 47, 148, 387, 498
StyI CCWWGG 1 cut(s) 400
TaaI ACNGT 1 cut(s) 535
TaiI ACGT 1 cut(s) 474
TasI AATT 2 cut(s) 15, 307
Tru1I TTAA 1 cut(s) 87
Tru9I TTAA 1 cut(s) 87
TscAI CASTG 1 cut(s) 238
TseFI GTSAC 2 cut(s) 55, 467
TseI GCWGC 2 cut(s) 42, 493
Tsp45I GTSAC 2 cut(s) 55, 467
TspDTI ATGAA 1 cut(s) 38
TspRI CASTG 1 cut(s) 238
XapI RAATTY 1 cut(s) 307
XspI CTAG 1 cut(s) 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.