Rorug05G0280900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0014701
Physical Location & Seq
Reverse (-)
31919549 .. 31926029
6481 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0280900.1

Sequence Viewer

Length: 2862 bp
ATGGTCGCCGCCATGGCAGCTGCAAGCACAACGATAATCCCAGTGAACGTGGGAGTTGTTGTAGACCTTGATGAACCGAGAGGGAAAATGTATTTGAGTTGCATCAAAATGGCGCTCTCGGATTTCTATGCTATCCATTCTGACTACAAGACTAGGCTCGTTTTGAACACCAGGAACTCCAATGGAGATGTGGTTGGAGCAGCTGCCGCAGCTTTACATCTGATAAAGAATGTAGAAGTGAAAGCCATCCTAGGACCGGTGTCATCCATGCAGGCAAGTTTCGTTGTTAATCTTGGAGAGCAAGCTCATGTACCCATCATATCATTTTCTGCAACAAGCCCTTCTCTTACTTCACTACGGAGCTCCTATTTTTTCCGATTTGCACAAAATGACTTGAACCAAGTGAAAGCTATAAGTGGAATTGTTCAGAACTTTGGATGGAGACGAGTTGTGCCCATCTACGTAGACAATATGTATGGGGAAGGAGTCATACCTTTTCTAATTGATGCCTTGCAAGAGGTTGATGTTCATATCCCCTACAGAAGTCTAATTCACGAACTAGCCACTGATGATCAAATTGAAAAAGAGCTTTACAAGTTGATGACGATGCAGACTCGAGTCTTCATTGTGCATATGATGCCTAATCTTTCCTCTCGGCTATTTGCCAAGGCAAAAGAGATCGGAATGATGAGTGAGGGTTTTGTTTGGATCACAACTAATAGTATTGGAAATAATTTAAAGTCTATGGCTTCAGTCCTTGATTCGATGCAAGGCCTATTAGGTGTAGAAACTTACATTCCAAGGACATTGAAGCTTAGACAATTCATATCAAGGTGGAAAAGGCAATTCCAACAAGATAATCCAAAAATCATTGGTGTTGAATTGGATGCTTTTGGATTGCGGGCATATGATGCTGCTTTTGCAGTAGCCATGGCCTTTGAGAAAGTTGCCAATGCAGGTTTCAAATTCCAAAAGAGGAATGCTTCTTCCTTCAACTCAACAGATCTTGATACTTTTGAGGTCTCTCATTATGGCTCAAAACTTTCTCATGCATTATCTATTATTACTTTCAAAGGTATGGCTGGAGACTTCAATCTTGTTGATAGGCAACTTCAGGTATCAACTTTTGAGATTGTTAATGTGAATGGTTATGGCGCAAGAACAGTTGGATATTGGACACCAGAAAACGGAAGGGTAGTAAAGCGGTTGAACTCAACAAGGAATAGAACAATATCAACTTCCAAGTGCGATCTTGGACCTATTATATGGCCTGGAGAGTCATTATCAGTTCCTAAAGGATGGGAGATCCCGACAAATGGTACCAAGTTAAGAATAGGAGTTCCTGTGAAGGAAGGTTTTCTAGAGTTTGTTAAAGTAACCAGCTGTCCAAGCACTAATACAGTTGACGTCACTGGGTTCAGTATTGACGTGTTTAAAGCCGTATTGGAGATCCTACCATATGCACTTCCTTATGAGTTTGTTCCCTTTGCAATGGCTAATGGAAGCAGCGCTGGCACATACAATGATTTGGTGTATCAAGTATATGCTGGGAAATTTGATGCTGTGGTTGGAGATGTAACAATTAGAGCAAATAGGTCATTGTATGTGGGCTTTACTATGCCATATACCGAATCTGGTGTAGTGATGGTTGTGCCAATACAAGACAAGAGGACCAAAAGTGCATGGGTTTTCTTGAAGCCTTTGACATGGGACCTTTGGATGACAACTTTGTGTTTTTTCATCTTTATTGGTTTTGTGATTTGGGTGCTTGAACATCGAATTAATGATGATTTTCGTGGTTCTCCCTCACATCAAGTTGGCACAAGCTTCTGGTTCTCTTTCTCAACCATGGTTTTCTCACAAAGGGAGAAAGTGGTTAGCAACTTGGCAAGATTTGTGATGATTATATGGATATTCGTTGTACTAATAGTGACACAGAGCTATACAGCTAGTCTAGCATCACTATTGACAGTACAACAACTCCAACCAACTGTTACCGATATAGATGATCTTTTGAGGAATAGAGATAACGTGGGCTACATGACGAGTTCTTATATTTATGGTCTCTTGAGACAAAAAGGTTTTCATGATTCCAGGCTTAAGGATTATGGAACTATGGAAGAAATAGATGAAGCTCTTTCAAAGGGTAGTGCAAATGGCGGTATTGCTGCTATTGTTCATGAAACCCCCTATATGAAGCTTTTTGTTGCAAAATATTGTTCCAAGTATACCATGATTGGACCTATATTTAAAACTGATGGCTTTGGCTTCGTATTTCCGAAAGGTTCTCCTCTTATACCCGATGTTTCACAAGCAATCCTTAATTTGACAGAAGGAGAGAAGATAATGAACATTGAAGACAAATGGATCAATAAAGAAAACAATTGTAAAGATCCAAGTACCGAGAAATTTTACAGCAGTAGTCTTGGCCTTGAGAGCTTTTGGGGCCTCTTTCTCATTGCTGGGGTTGCTTCAATATTCGCTCTCCTCATATTTGCAACTTCCTTCCTTCACAAGCATAAGCAAGTTTTGATGTCCTCTGATTCAAGAGCTTCCAAGTGGAAAAGGATTAGGACCATGTTCAAGATTTTCAATGAAAAAGAACTTAGCTCTCATACTTTTAAAAGCAGTAGGCATGAAGCTAGGATCGCTGGTGCTCCAGATCATGAGCTGAATGCCTCACCAAATAACAACTGGCCAGAAAGTCCATTCAGCTATACCAACCAGACGGATGCAACTTCTGTATTCTACGGAGAGCAAGAAACACCATCTCACGGTCAAGCATCTCCGGAAATCGTTCCAACTTTTGAGCTTGCTGCTTTCACTAATCAAGAAATGCATGCAACTCCGGAAACAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

953

Amino Acids

106.45

Weight (kDa)

7.65

Isoelectric Point (pI)

36.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peripla_BP_6 PF13458 14 - 316 2.1e-19 Periplasmic binding protein
ANF_receptor PF01094 34 - 383 2.1e-78 Receptor family ligand binding region
Lig_chan-Glu_bd PF10613 465 - 551 2.5e-07 Ligated ion channel L-glutamate- and glycine-binding site
SBP_bac_3 PF00497 469 - 791 3e-10 Bacterial extracellular solute-binding proteins, family 3
Lig_chan PF00060 569 - 824 2e-41 Ligand-gated ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1410
Acc36I ACCTGC 1 cut(s) 947
Acc65I GGTACC 1 cut(s) 1319
AccB1I GGYRCC 1 cut(s) 1319
AccI GTMKAC 3 cut(s) 63, 465, 2228
AccIII TCCGGA 2 cut(s) 2788, 2848
AciI CCGC 5 cut(s) 9, 207, 901, 1204, 2160
AclWI GGATC 6 cut(s) 716, 1300, 1444, 2375, 2387, 2654
AcoI YGGCCR 1 cut(s) 2696
AcsI RAATTY 3 cut(s) 965, 1553, 2408
AcuI CTGAAG 2 cut(s) 735, 1097
AcyI GRCGYC 1 cut(s) 1407
AfaI GTAC 5 cut(s) 312, 1321, 1923, 1974, 2401
AfeI AGCGCT 1 cut(s) 1510
AfiI CCNNNNNNNGG 4 cut(s) 256, 1187, 1316, 2774
AflII CTTAAG 1 cut(s) 2099
AflIII ACRYGT 1 cut(s) 1428
AgeI ACCGGT 1 cut(s) 256
AjiI CACGTC 1 cut(s) 1429
AjnI CCWGG 3 cut(s) 170, 1270, 2093
AjuI GAANNNNNNNTTGG 2 cut(s) 944, 976
Alw21I GWGCWC 2 cut(s) 365, 2659
Alw26I GTCTC 5 cut(s) 436, 1027, 1080, 2065, 2069
AlwI GGATC 6 cut(s) 716, 1300, 1444, 2375, 2387, 2654
Ama87I CYCGRG 1 cut(s) 615
Aor13HI TCCGGA 2 cut(s) 2788, 2848
Aor51HI AGCGCT 1 cut(s) 1510
AoxI GGCC 6 cut(s) 772, 933, 1268, 2428, 2446, 2696
ApeKI GCWGC 9 cut(s) 17, 20, 200, 203, 209, 914, 1506, 2168, 2816
ApoI RAATTY 3 cut(s) 965, 1553, 2408
AseI ATTAAT 1 cut(s) 1782
AsiGI ACCGGT 1 cut(s) 256
Asp700I GAANNNNTTC 2 cut(s) 1356, 2796
Asp718I GGTACC 1 cut(s) 1319
AspA2I CCTAGG 1 cut(s) 250
AspLEI GCGC 3 cut(s) 115, 1157, 1511
AspS9I GGNCC 7 cut(s) 254, 1256, 1671, 1711, 2240, 2446, 2574
AsuHPI GGTGA 1 cut(s) 2673
AvaI CYCGRG 1 cut(s) 615
AvaII GGWCC 6 cut(s) 254, 1256, 1671, 1711, 2240, 2574
AvrII CCTAGG 1 cut(s) 250
BaeGI GKGCMC 1 cut(s) 456
BalI TGGCCA 1 cut(s) 2698
BanI GGYRCC 1 cut(s) 1319
BanII GRGCYC 1 cut(s) 365
BarI GAAGNNNNNNTAC 2 cut(s) 474, 506
BbsI GAAGAC 2 cut(s) 613, 2364
Bbv12I GWGCWC 2 cut(s) 365, 2659
BbvI GCAGC 9 cut(s) 7, 29, 190, 212, 221, 901, 1518, 2155, 2803
BccI CCATC 8 cut(s) 254, 323, 432, 464, 1293, 1639, 2252, 2776
BceAI ACGGC 1 cut(s) 1424
BciT130I CCWGG 3 cut(s) 172, 1272, 2095
BclI TGATCA 1 cut(s) 571
BcoDI GTCTC 5 cut(s) 436, 1027, 1080, 2065, 2069
BfaI CTAG 7 cut(s) 153, 251, 560, 1361, 1950, 1955, 2643
BfmI CTRYAG 1 cut(s) 538
BfoI RGCGCY 2 cut(s) 116, 1512
BfrI CTTAAG 1 cut(s) 2099
BfuAI ACCTGC 1 cut(s) 947
BglI GCCNNNNNGGC 1 cut(s) 14
BglII AGATCT 1 cut(s) 1003
BlnI CCTAGG 1 cut(s) 250
Bme1390I CCNGG 3 cut(s) 172, 1272, 2095
Bme18I GGWCC 6 cut(s) 254, 1256, 1671, 1711, 2240, 2574
BmeT110I CYCGRG 1 cut(s) 615
BmgBI CACGTC 1 cut(s) 1429
BmgT120I GGNCC 7 cut(s) 254, 1256, 1671, 1711, 2240, 2446, 2574
BmiI GGNNCC 3 cut(s) 1321, 1712, 2447
BmrFI CCNGG 3 cut(s) 172, 1272, 2095
BmrI ACTGGG 2 cut(s) 35, 1422
BmuI ACTGGG 2 cut(s) 35, 1422
BoxI GACNNNNGTC 2 cut(s) 259, 617
BpiI GAAGAC 2 cut(s) 613, 2364
BpmI CTGGAG 3 cut(s) 1104, 1293, 2643
BpuEI CTTGAG 2 cut(s) 2089, 2453
BsaAI YACGTR 1 cut(s) 463
BsaHI GRCGYC 1 cut(s) 1407
BsaI GGTCTC 2 cut(s) 1027, 2069
BsaJI CCNNGG 6 cut(s) 12, 250, 666, 800, 930, 1848
BsaWI WCCGGW 3 cut(s) 256, 2788, 2848
BsaXI ACNNNNNCTCC 8 cut(s) 45, 75, 177, 207, 1329, 1359, 1965, 1995
Bsc4I CCNNNNNNNGG 4 cut(s) 256, 1187, 1316, 2774
Bse118I RCCGGY 1 cut(s) 256
Bse1I ACTGG 3 cut(s) 41, 1417, 2699
Bse3DI GCAATG 2 cut(s) 1497, 2457
BseAI TCCGGA 2 cut(s) 2788, 2848
BseBI CCWGG 3 cut(s) 172, 1272, 2095
BseDI CCNNGG 6 cut(s) 12, 250, 666, 800, 930, 1848
BseGI GGATG 7 cut(s) 246, 263, 443, 892, 1304, 1725, 2737
BseLI CCNNNNNNNGG 4 cut(s) 256, 1187, 1316, 2774
BseMI GCAATG 2 cut(s) 1497, 2457
BseNI ACTGG 3 cut(s) 41, 1417, 2699
BseRI GAGGAG 2 cut(s) 2280, 2477
BseSI GKGCMC 1 cut(s) 456
BseXI GCAGC 9 cut(s) 7, 29, 190, 212, 221, 901, 1518, 2155, 2803
BseYI CCCAGC 2 cut(s) 1547, 2462
BshFI GGCC 6 cut(s) 774, 935, 1270, 2430, 2448, 2698
BshNI GGYRCC 1 cut(s) 1319
BshTI ACCGGT 1 cut(s) 256
BsiHKAI GWGCWC 2 cut(s) 365, 2659
BsiHKCI CYCGRG 1 cut(s) 615
BsiSI CCGG 3 cut(s) 257, 2789, 2849
BslFI GGGAC 1 cut(s) 1724
BslI CCNNNNNNNGG 4 cut(s) 256, 1187, 1316, 2774
BsmAI GTCTC 5 cut(s) 436, 1027, 1080, 2065, 2069
BsmBI CGTCTC 1 cut(s) 436
BsmFI GGGAC 1 cut(s) 1724
BsmI GAATGC 2 cut(s) 985, 2680
BsnI GGCC 6 cut(s) 774, 935, 1270, 2430, 2448, 2698
Bso31I GGTCTC 2 cut(s) 1027, 2069
BsoBI CYCGRG 1 cut(s) 615
Bsp1286I GDGCHC 3 cut(s) 365, 456, 2659
Bsp13I TCCGGA 2 cut(s) 2788, 2848
Bsp19I CCATGG 3 cut(s) 12, 930, 1848
BspACI CCGC 5 cut(s) 9, 207, 901, 1204, 2160
BspANI GGCC 6 cut(s) 774, 935, 1270, 2430, 2448, 2698
BspEI TCCGGA 2 cut(s) 2788, 2848
BspHI TCATGA 3 cut(s) 2086, 2179, 2665
BspLI GGNNCC 3 cut(s) 1321, 1712, 2447
BspMI ACCTGC 1 cut(s) 947
BspPI GGATC 6 cut(s) 716, 1300, 1444, 2375, 2387, 2654
BspT107I GGYRCC 1 cut(s) 1319
BspTI CTTAAG 1 cut(s) 2099
BspTNI GGTCTC 2 cut(s) 1027, 2069
BsrDI GCAATG 2 cut(s) 1497, 2457
BsrFI RCCGGY 1 cut(s) 256
BsrI ACTGG 3 cut(s) 41, 1417, 2699
BssAI RCCGGY 1 cut(s) 256
BssECI CCNNGG 6 cut(s) 12, 250, 666, 800, 930, 1848
BssNAI GTATAC 1 cut(s) 2229
BssNI GRCGYC 1 cut(s) 1407
BssT1I CCWWGG 6 cut(s) 12, 250, 666, 800, 930, 1848
Bst1107I GTATAC 1 cut(s) 2229
Bst2UI CCWGG 3 cut(s) 172, 1272, 2095
Bst4CI ACNGT 5 cut(s) 1165, 1402, 1972, 1993, 2777
BstACI GRCGYC 1 cut(s) 1407
BstAFI CTTAAG 1 cut(s) 2099
BstAPI GCANNNNNTGC 2 cut(s) 637, 911
BstBAI YACGTR 1 cut(s) 463
BstC8I GCNNGC 7 cut(s) 25, 273, 303, 903, 1513, 2814, 2841
BstDEI CTNAG 2 cut(s) 815, 2606
BstDSI CCRYGG 3 cut(s) 12, 930, 1848
BstF5I GGATG 7 cut(s) 246, 263, 443, 892, 1304, 1725, 2737
BstH2I RGCGCY 2 cut(s) 116, 1512
BstHHI GCGC 3 cut(s) 115, 1157, 1511
BstMAI GTCTC 5 cut(s) 436, 1027, 1080, 2065, 2069
BstNI CCWGG 3 cut(s) 172, 1272, 2095
BstNSI RCATGY 1 cut(s) 2843
BstPAI GACNNNNGTC 2 cut(s) 259, 617
BstSCI CCNGG 3 cut(s) 170, 1270, 2093
BstSFI CTRYAG 1 cut(s) 538
BstSLI GKGCMC 1 cut(s) 456
BstSNI TACGTA 1 cut(s) 463
BstV1I GCAGC 9 cut(s) 7, 29, 190, 212, 221, 901, 1518, 2155, 2803
BstV2I GAAGAC 2 cut(s) 613, 2364
BstX2I RGATCY 4 cut(s) 1003, 1305, 1449, 2392
BstYI RGATCY 4 cut(s) 1003, 1305, 1449, 2392
BstZ17I GTATAC 1 cut(s) 2229
BsuRI GGCC 6 cut(s) 774, 935, 1270, 2430, 2448, 2698
BtgI CCRYGG 3 cut(s) 12, 930, 1848
BtrI CACGTC 1 cut(s) 1429
BtsCI GGATG 7 cut(s) 246, 263, 443, 892, 1304, 1725, 2737
BtsIMutI CAGTG 3 cut(s) 48, 564, 1410
BveI ACCTGC 1 cut(s) 947
Cac8I GCNNGC 7 cut(s) 25, 273, 303, 903, 1513, 2814, 2841
CciI TCATGA 3 cut(s) 2086, 2179, 2665
CfoI GCGC 3 cut(s) 115, 1157, 1511
Cfr10I RCCGGY 1 cut(s) 256
Cfr13I GGNCC 7 cut(s) 254, 1256, 1671, 1711, 2240, 2446, 2574
Csp6I GTAC 5 cut(s) 311, 1320, 1922, 1973, 2400
CspAI ACCGGT 1 cut(s) 256
CviQI GTAC 5 cut(s) 311, 1320, 1922, 1973, 2400
DdeI CTNAG 2 cut(s) 815, 2606
DraI TTTAAA 4 cut(s) 738, 1435, 2251, 2623
EaeI YGGCCR 1 cut(s) 2696
Ecl136II GAGCTC 1 cut(s) 363
Eco105I TACGTA 1 cut(s) 463
Eco130I CCWWGG 6 cut(s) 12, 250, 666, 800, 930, 1848
Eco147I AGGCCT 1 cut(s) 774
Eco24I GRGCYC 1 cut(s) 365
Eco31I GGTCTC 2 cut(s) 1027, 2069
Eco47I GGWCC 6 cut(s) 254, 1256, 1671, 1711, 2240, 2574
Eco47III AGCGCT 1 cut(s) 1510
Eco53kI GAGCTC 1 cut(s) 363
Eco57I CTGAAG 2 cut(s) 735, 1097
Eco88I CYCGRG 1 cut(s) 615
EcoICRI GAGCTC 1 cut(s) 363
EcoO109I RGGNCCY 2 cut(s) 1711, 2446
EcoRII CCWGG 3 cut(s) 170, 1270, 2093
EcoT14I CCWWGG 6 cut(s) 12, 250, 666, 800, 930, 1848
EcoT22I ATGCAT 2 cut(s) 1054, 2841
EcoT38I GRGCYC 1 cut(s) 365
ErhI CCWWGG 6 cut(s) 12, 250, 666, 800, 930, 1848
Esp3I CGTCTC 1 cut(s) 436
FalI AAGNNNNNCTT 2 cut(s) 2304, 2336
FaqI GGGAC 1 cut(s) 1724
FauI CCCGC 1 cut(s) 894
FauNDI CATATG 3 cut(s) 633, 907, 1459
FbaI TGATCA 1 cut(s) 571
FblI GTMKAC 3 cut(s) 63, 465, 2228
FokI GGATG 7 cut(s) 233, 250, 450, 899, 1311, 1732, 2744
FriOI GRGCYC 1 cut(s) 365
FspBI CTAG 7 cut(s) 153, 251, 560, 1361, 1950, 1955, 2643
GlaI GCGC 3 cut(s) 114, 1156, 1510
GsaI CCCAGC 2 cut(s) 1551, 2466
GsuI CTGGAG 3 cut(s) 1104, 1293, 2643
HaeII RGCGCY 2 cut(s) 116, 1512
HaeIII GGCC 6 cut(s) 774, 935, 1270, 2430, 2448, 2698
HapII CCGG 3 cut(s) 257, 2789, 2849
HhaI GCGC 3 cut(s) 115, 1157, 1511
Hin1I GRCGYC 1 cut(s) 1407
Hin6I GCGC 3 cut(s) 113, 1155, 1509
HinP1I GCGC 3 cut(s) 113, 1155, 1509
HincII GTYRAC 1 cut(s) 1405
HindII GTYRAC 1 cut(s) 1405
HindIII AAGCTT 3 cut(s) 812, 1825, 2198
HinfI GANTC 8 cut(s) 486, 613, 618, 761, 1277, 1631, 2090, 2543
HpaII CCGG 3 cut(s) 257, 2789, 2849
HphI GGTGA 1 cut(s) 2673
Hpy166II GTNNAC 5 cut(s) 46, 64, 466, 1405, 2229
Hpy188I TCNGA 8 cut(s) 121, 142, 222, 377, 429, 683, 2280, 2542
Hpy8I GTNNAC 5 cut(s) 46, 64, 466, 1405, 2229
HpyAV CCTTC 9 cut(s) 351, 476, 1000, 1185, 1342, 1346, 2327, 2515, 2519
HpyCH4III ACNGT 5 cut(s) 1165, 1402, 1972, 1993, 2777
HpyCH4IV ACGT 5 cut(s) 48, 462, 1407, 1428, 2031
HpyF3I CTNAG 2 cut(s) 815, 2606
HpySE526I ACGT 5 cut(s) 48, 462, 1407, 1428, 2031
Hsp92I GRCGYC 1 cut(s) 1407
HspAI GCGC 3 cut(s) 113, 1155, 1509
Kpn2I TCCGGA 2 cut(s) 2788, 2848
KpnI GGTACC 1 cut(s) 1323
Ksp22I TGATCA 1 cut(s) 571
LmnI GCTCC 4 cut(s) 197, 360, 368, 2662
Lsp1109I GCAGC 9 cut(s) 7, 29, 190, 212, 221, 901, 1518, 2155, 2803
MaeI CTAG 7 cut(s) 153, 251, 560, 1361, 1950, 1955, 2643
MaeII ACGT 5 cut(s) 48, 462, 1407, 1428, 2031
MaeIII GTNAC 5 cut(s) 1375, 1408, 1576, 1930, 1993
MboII GAAGA 5 cut(s) 613, 978, 2132, 2353, 2369
MfeI CAATTG 1 cut(s) 2383
MflI RGATCY 4 cut(s) 1003, 1305, 1449, 2392
MhlI GDGCHC 3 cut(s) 365, 456, 2659
MlsI TGGCCA 1 cut(s) 2698
MluNI TGGCCA 1 cut(s) 2698
MlyI GAGTC 4 cut(s) 495, 607, 627, 1286
MmeI TCCRAC 6 cut(s) 175, 874, 1147, 1549, 2008, 2825
Mox20I TGGCCA 1 cut(s) 2698
Mph1103I ATGCAT 2 cut(s) 1054, 2841
MroI TCCGGA 2 cut(s) 2788, 2848
MroXI GAANNNNTTC 2 cut(s) 1356, 2796
MscI TGGCCA 1 cut(s) 2698
MslI CAYNNNNRTG 1 cut(s) 107
Msp20I TGGCCA 1 cut(s) 2698
MspA1I CMGCKG 3 cut(s) 20, 203, 1383
MspCI CTTAAG 1 cut(s) 2099
MspI CCGG 3 cut(s) 257, 2789, 2849
MspR9I CCNGG 3 cut(s) 172, 1272, 2095
MunI CAATTG 1 cut(s) 2383
Mva1269I GAATGC 2 cut(s) 985, 2680
MvaI CCWGG 3 cut(s) 172, 1272, 2095
NcoI CCATGG 3 cut(s) 12, 930, 1848
NdeI CATATG 3 cut(s) 633, 907, 1459
NlaIV GGNNCC 3 cut(s) 1321, 1712, 2447
NmeAIII GCCGAG 1 cut(s) 634
NmuCI GTSAC 2 cut(s) 1408, 1930
NsiI ATGCAT 2 cut(s) 1054, 2841
NspI RCATGY 1 cut(s) 2843
PaeI GCATGC 1 cut(s) 2843
PaeR7I CTCGAG 1 cut(s) 615
PagI TCATGA 3 cut(s) 2086, 2179, 2665
PceI AGGCCT 1 cut(s) 774
PctI GAATGC 2 cut(s) 985, 2680
PdmI GAANNNNTTC 2 cut(s) 1356, 2796
PfeI GAWTC 4 cut(s) 761, 1631, 2090, 2543
PinAI ACCGGT 1 cut(s) 256
PleI GAGTC 4 cut(s) 494, 607, 626, 1285
PpsI GAGTC 4 cut(s) 494, 607, 626, 1285
Ppu21I YACGTR 1 cut(s) 463
PpuMI RGGWCCY 1 cut(s) 1711
PshAI GACNNNNGTC 2 cut(s) 259, 617
PshBI ATTAAT 1 cut(s) 1782
Psp124BI GAGCTC 1 cut(s) 365
Psp5II RGGWCCY 1 cut(s) 1711
Psp6I CCWGG 3 cut(s) 170, 1270, 2093
PspFI CCCAGC 2 cut(s) 1547, 2462
PspGI CCWGG 3 cut(s) 170, 1270, 2093
PspN4I GGNNCC 3 cut(s) 1321, 1712, 2447
PspPI GGNCC 7 cut(s) 254, 1256, 1671, 1711, 2240, 2446, 2574
PspPPI RGGWCCY 1 cut(s) 1711
PspXI VCTCGAGB 1 cut(s) 615
PsuI RGATCY 4 cut(s) 1003, 1305, 1449, 2392
PvuII CAGCTG 3 cut(s) 20, 203, 1383
RsaI GTAC 5 cut(s) 312, 1321, 1923, 1974, 2401
RsaNI GTAC 5 cut(s) 311, 1320, 1922, 1973, 2400
RseI CAYNNNNRTG 1 cut(s) 107
SacI GAGCTC 1 cut(s) 365
Sau96I GGNCC 7 cut(s) 254, 1256, 1671, 1711, 2240, 2446, 2574
SchI GAGTC 4 cut(s) 495, 607, 627, 1286
ScrFI CCNGG 3 cut(s) 172, 1272, 2095
SduI GDGCHC 3 cut(s) 365, 456, 2659
SfcI CTRYAG 1 cut(s) 538
Sfr274I CTCGAG 1 cut(s) 615
SinI GGWCC 6 cut(s) 254, 1256, 1671, 1711, 2240, 2574
SlaI CTCGAG 1 cut(s) 615
SmiMI CAYNNNNRTG 1 cut(s) 107
SmlI CTYRAG 4 cut(s) 615, 2068, 2099, 2432
SmoI CTYRAG 4 cut(s) 615, 2068, 2099, 2432
SnaBI TACGTA 1 cut(s) 463
SphI GCATGC 1 cut(s) 2843
SseBI AGGCCT 1 cut(s) 774
SsiI CCGC 5 cut(s) 9, 207, 901, 1204, 2160
SspI AATATT 2 cut(s) 2216, 2478
SspMI CTAG 7 cut(s) 153, 251, 560, 1361, 1950, 1955, 2643
SstI GAGCTC 1 cut(s) 365
StuI AGGCCT 1 cut(s) 774
StyD4I CCNGG 3 cut(s) 170, 1270, 2093
StyI CCWWGG 6 cut(s) 12, 250, 666, 800, 930, 1848
TaaI ACNGT 5 cut(s) 1165, 1402, 1972, 1993, 2777
TaiI ACGT 5 cut(s) 51, 465, 1410, 1431, 2034
TaqI TCGA 3 cut(s) 616, 764, 1777
TatI WGTACW 2 cut(s) 1921, 1972
TauI GCSGC 2 cut(s) 11, 209
TfiI GAWTC 4 cut(s) 761, 1631, 2090, 2543
TscAI CASTG 3 cut(s) 48, 571, 1417
TseFI GTSAC 2 cut(s) 1408, 1930
TseI GCWGC 9 cut(s) 17, 20, 200, 203, 209, 914, 1506, 2168, 2816
Tsp45I GTSAC 2 cut(s) 1408, 1930
TspGWI ACGGA 4 cut(s) 373, 1203, 2744, 2766
TspRI CASTG 3 cut(s) 48, 571, 1417
Vha464I CTTAAG 1 cut(s) 2099
VpaK11BI GGWCC 6 cut(s) 254, 1256, 1671, 1711, 2240, 2574
VspI ATTAAT 1 cut(s) 1782
XapI RAATTY 3 cut(s) 965, 1553, 2408
XbaI TCTAGA 1 cut(s) 1360
XceI RCATGY 1 cut(s) 2843
XcmI CCANNNNNNNNNTGG 2 cut(s) 187, 2691
XhoI CTCGAG 1 cut(s) 615
XmaJI CCTAGG 1 cut(s) 250
XmiI GTMKAC 3 cut(s) 63, 465, 2228
XmnI GAANNNNTTC 2 cut(s) 1356, 2796
XspI CTAG 7 cut(s) 153, 251, 560, 1361, 1950, 1955, 2643
ZraI GACGTC 1 cut(s) 1408
Zsp2I ATGCAT 2 cut(s) 1054, 2841
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.