Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000006

Orthogroup ID

OG0003600

Physical Location & Seq

Genomic Coordinates Reverse (-)

1782669 .. 1787839

Gene Structure 5171 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug06G0013500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5064 bp

ATGCTCCAGTTTCAATCTGTTGAAGATATGCAGAATGTCTGGGCGTTGGGCACCATCAGACTGCCTCTTGGGCTCCTCCGCTTAATCAAATGGTCTCCTACATTCTCGCCATCCACCTACAAAAACTCTTTTGCACAAGTTTGGGTCCGCTTCTGGGACCTTGGTTTTGATTTCTGGGAACAACAAACGCTATTCGAAATTGCGAGAGGGATTGGCATGCCTCTGAAACTTGACCCCAGAACTGTCAACCGCTCTCTGGGCCTCTATGCCAGGATACTCATAGATGTTGATTTTGCTCAGCCTTTGTTATCTACCCTCCGAGTGACCCGGTCTGACGGAACTCATGTTCTGGTGGGTATTGAATATGAGTCTGCCCCTGTTGTGTGCTCCATTTGCAGCAACATTGGTCATTTGGAGACAAAGTGTAGGGTCTCTACAGTGGAGGTTGAACCCATTTCTCGACGTGGGAGGTCTAGGATTCATACTAGACGGAGGAGACGCCCCTCTCTTCCACGCCATGATTCACCCACGGATCCAGGCCCCCTGTCTGAGGGTGTAGCCGCTCCTGCTCATGCTGCCATGCCCACACACCCCCAAACCATCACACCAGTATGTATACCCTCTGATCAACCAGAGTTTCCGCCGATCCATCAACGAGTCACCACTCCGCCGGAGCCGGCCATTGTTCATCGTACTTCCATCCATACCTCCTCCTCTGACAGCATTCCACCAGGGTTTCAAACTGCGAATCTCTATGCGGCAGCCCCATGTGAACATTCCTCCTCCACACCTATTGCCTCGGAAGTTGACCCAAACATGCTGGCTCAATCTGCTTTTGAGGAAGGCTCTGTTTCGGCAGGTGTTGAAGAATATCTTATCCCTAGGGAAGAGAGAGAAGAAGGAGAGTTCACGCCTGTCGTCTCGAAAAGAACCAAGAAACAGTTAAAACAGGTTTTAAAATCGAGAGGTATCGCAAATATTGATACTCAACGGGCTCTAACTTCCATGGTTCGTATTCATAAACCCATAATTCTCTGCTTGTCCGAACCATTTGTTCCTCTATCTGCAATTCCGGATGCTTTTTGGCGTTCCTTGGGTTTGTTTCCTTTTGCTACTAATGACCGGGGACTTTCAGACCCCAACCTTTGGTTTCTATGTCACCCAGATATCAAACCAGTTTTACTTTCTGCTACTGCACAACAAATTTCAGTATCCTGTTCTCTTGATGGTGTCTCTTATATCCTCTCCACTGTCTACGCAAAGACTACTATTGCTGGCAGACGGTTATTGTGGCATGATCTTAGTACAATTGCCTCTTCCTATGTTCAAGGCCCTTGGCTGGTATTCGGTGACTTTAACTGTGTTATTGGGGCACATGAAAAGCGTGGTGGAAACCCGCCTAATGCTCGCTCATGCATGGATTTCCAAAGCATGTGCACTACTTGCAGTCTACTCGACATACCAACAAAAGGGCTTTCTTATACTTGGACTGACAAAAGAACTTATACTCGCCTCGATCGAGCGTTAGCTAACTCTGCTTGGATGGATTCTTGGGCTTCTGTGTCCTGTCGTACCCTCACGAGAGCTTCCTCTGACCACTGCCCTATTCTAATGTCTCATTCCAGATTGGCCTTCATCCCAAAGTCTCCTTTCCGGTTTAAACGCATGTGGCTTCTCTTCCCTGGTTTCTTGCAGTTAGTCTGTCATTTCTGGACTACCCTGCATCATGTGGGCTGTCCTATGATATCCTTTGCATGCAAGTTACGTGCTCTAAAGAACTGGCTTAAGACATGGAATGGCTCAGTGTTTGGGGATATTAATGATAGAGTTAATTCCTCCAGATCTGTTCTTGATACAGTGCAGTCGGAAATCTCGGACTTTGGCCAAACTGAGGACCGTTTAAACAGGGAAAGTCAGGCCCTCACTAGCCTCAATGATGCCCTAGCTCTTCAGGACTGCCTCCTAAAAGAGAAAGCTCGAGTTCGTTGGTTACAAGATGGTGACCGCAATAATTCCTTCCTTCACAATATGGTCAAAATCCGCCATCTCAAGAATTCAATTACTTCTCTTCGTGTGGGTCAACATCGCATTTTTGATCATGAGTTGATTGCAGATCATGCTGTACGCCACTTTAGCTCAACTTTCACCCAAGACTGCGATATAGTTAACACTGGTCTGGTGGAACGCCTCATCCCAAACTTGGTAACTGACGACGAAAATCTTTTACTCACTGCTCTTCCTTCTGCTGAGGAAATCAAATCTGTTGTTTTCTCCATGGATGGGTCAAGTGCTCCTGGGCCTGATGGTTTTGGTGGTTGCTTCTTCCAATTCTGCTGGGAAGTGATCTCAATCGATGTCATCTCTGTTGTTCAAAGCTTCTTTCAGACTGGCTTTATTCCACCTCACTTTAACTCGAGTTTAATCACACTCATCCCTAAGAAACAGGAGGCGGACAACCTTTCTGATTTTCGACCCATTGCGTTAGCAAACTTTGTCTTCAAGATTATTACTAAAATCCTTGCTGACAGACTTAGCGAGGTTGCTGCTAGAATTATCTCCCCTGAGCAGAGCGCTTTTATAAAAGGACGTTCAATTGTTGACTCCATCTCTCTGACATCAGAATGCATCAATCTGCTGGATCGTAAGTGTGATGGAGGTAATATTGCTTTAAAGCTTGACATTAAAAAAGCTTTTGACACCATCGATTGGTCCTTCCTGCTTCGGGTTCTTACCTCTTTTGGTTTCGACTCACGCTTTATCTCTTGGGTCACTACTATCCTGCATTCTGCTTATCTTTCTATTCTAATAAATGGGGAACCGGCTGGCTTCTTCCCTTGCTCGCGAGGTGTCCGGCAGGGGGACCCTCTTTCCCCTCTACTTTTTTGTCTTGCCGAAGAGGTGTTAAGCCGGGGTATCTCTAACCTTGTGGCTATGGGAAAGATCACACGCTTTGCAGCGCCCAGTAAGGTTAATTCCCCGTCCCATGTACTATTTGTTGATGATGTTATGGTCTTTATGCAAGGAGACAAAAAGAGTTTACGGAGTCTTTTGCGTTTTCTTGAGGACTATGCTCGGAATTCAGGCCAAGTTATCAATAAAGCTAAATCTTCAATCTACCTGGGTCGGTTTGCACGACCTAGAGCTACATTTATCCAACATCTTCTGGGCATCAGGGAAGGTAGTTTGCCTTTCACCTATCTCGGTGTCCCAGTCTTTCAAGGGTGCCCAAAGCAAACTTATTTCCGGGGTATTGCAGACAAAGTAAGGTGTAAACTTAGTACTTGGAAAGGTAGGCACCTTTCCCAGGCCGCGAGGTTGCAATTAATCAATTCTGTTATCCACAGTATTCTTGTGTATAGCTTTCAGATCTATGAGTGGCCCCGCTCTATTCTCTATAAGGTTCAAGGTTGGATGAGAAATTTCTTTTGGGTGGGAGACCCTTTAAAGTCTAGCTGTTGGGTGGCTTGGTCAACCTGTTGTCTTCCTCAAAATGAAGGTGGCCTGGGTCTTAAAAACATTGTCATCCTTAATCAAAGCCTTCTTCTTAAACGTTGCTGGGAGGTAGCCAATGGTGCTTCTCCTTCAGCCTCTTTTTTGAAGGGTCGTTTTGTTGATCCTGGCCTCACTCCTACTGTTGTTTACCGTAAGTCTTCCATTTGGACTGGTCTGAAAAAACTTTGGCCGACTCTTACTGAAAATGTTCATTGGTTAATCGGAAACGGTCTCAAGACTTCCTTTTGGAAGGACAATTGGGTTGGTCAACCTCTCTTTCAACTCTGTAATATTGATGTGTCACTTCTCCCTCATCTAAATGCAAGTGTCAAGGATTACATTGTTGATCATCGCTGGGTTCTGCCTCCTTTGTTTTCTCAACTTTTTCCTTCAGTGGCTGATTGCATTCATGAGATTGTCCTCCCCCTGGAGAATACTGAGGACCAAGTCATCTGGTCAGGGTCATCCTCAGGTAAACTTTCTGCCCATGATGCCTATGATTTCCTTCTTCCTCATCATCCTGTGGTTAACTGGGGAAAACGTATATGGCATCCTGCTATTCAACCAAGGAAAAGTTTGCTTACTTGGAAAGTGCTTCATGAGAAAGTCCCAGTTGATGCCTTGTTACAAAGGCGTGGTCACTCTCTCTGCTCGATGTGTTCTTTCTGCAATTCCTCTCCGGAAACGTTGGATCATCTTTTCTTGCAGTGTAGAGTGGTCCTTCAAATTTGGGATTGGATGCTACACCTTTTCCATCTTCGTTCCCCAACTCCCCTCTCTATCCATGCTCTTATTTCTGTTGACTTCACCACTAGTATCCAACGCTCTGGTGTCACTCTTTGGAAACTTGTTTTTTGCAACCTACTTTGGTCTATTTGGCGAGAAAGAAACAAGGTGCGGTTTGACAACACGAGATTTTGTGCAATTCATCTCAAAAGAAGCTTCATCCTCTCCCTGCGTGACTCAGCTTCTCTAAGCTTCAAACCATGTACCACATACACCTCATCTCTGCCCATCTTTAATTTTTTGGGCCTCTCATCTTTAAACATTCGTGCTCCGAGATTTATTCCAGTCACTTGGGTCTGCCCCCCTCCTCCTTGGCTCAAAGCCAACACTGATGGTTCATTTCATGATGTCAACTCTGCTGGTTATGGTGGCATCTTCAGGGACAACCTTGGCCTCTTCCAGGGAGCTTTTTCTGCAAAGGTTTCTGTCCCTTGTGCCATTGATGCGGAGGTTCTCGCAGTGATCGAGGCTTTGAGACTCGCCTGGGTTCGCAATTGGACTCACATATGGATTGAAACGGATTCCTCTCTTGTGGTGACTTATTTTCAGCATCCCCATATGGTTCCTTGGCGTTTAAGAGCTCAATGGCTTAATTGTTTGCATTTCACTCGTCAGATTAACTTCCGGGTGTCTCACATCTTTAGAGAAGGCAATGCAGTAGCAGATCGTCTTGCTCGATATGGTGCTTCAACTGATGGGTATATGTGGTGGAACTCTCTACCTCCTTTCATCTCTGACCATTATGGTAGGGATTTGTCATCCCTGGTTTCCTACAGATTCAGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006996 GO:0007610 GO:0007622 GO:0007623 GO:0008150 GO:0009628 GO:0009657 GO:0009658 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016043 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0048511 GO:0048512 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0071840 GO:0080090 GO:0080167 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

1687

Amino Acids

190.45

Weight (kDa)

9.09

Isoelectric Point (pI)

45.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-CCHC_4	PF14392	96 - 143	9.6e-06	Zinc knuckle
RVT_1	PF00078	811 - 1070	5.8e-38	Reverse transcriptase (RNA-dependent DNA polymerase)
zf-RVT	PF13966	1325 - 1408	2.8e-20	zinc-binding in reverse transcriptase
RVT_3	PF13456	1535 - 1653	2e-16	Reverse transcriptase-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0003600)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G16290 AT2G16300 AT2G16365 AT2G16365 AT2G16365 AT2G16365 AT2G16365 AT2G16365 AT4G34550 AT4G34550
fragaria_vesca	FvH4_2g09190 FvH4_2g09190 FvH4_2g09190 FvH4_2g09190
malus_domestica	MD05G1064100.v1.1 MD10G1072800.v1.1
prunus_persica	Prupe.8G094200_v2.0.a1 Prupe.8G094200_v2.0.a1 Prupe.8G094200_v2.0.a1
pyrus_communis	pycom05g05530 pycom10g05750
rosa_chinensis	RchiOBHm_Chr6g0265031
rosa_laevigata	RLG00000014214
rosa_multiflora	Rmu_sc0002934.1_g000016 Rmu_sc0011803.1_g000002 Rmu_sc0042979.1_g000001
rosa_roxburghii	Rroxscaffold_175G00432110 Rroxscaffold_7G00203080
rosa_rugosa	Rorug06G0013500 Rorug06G0013600
rosa_samantha	Rh6AG136300 Rh6BG133200 Rh6CG131700 Rh6DG119000
rosa_wichuraiana	Rw6G011760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2579
AarI	CACCTGC	1 cut(s)	848
Acc36I	ACCTGC	1 cut(s)	848
AccB1I	GGYRCC	3 cut(s)	50, 3222, 3294
AccB7I	CCANNNNNTGG	2 cut(s)	1146, 2706
AccBSI	CCGCTC	3 cut(s)	252, 563, 3384
AccI	GTMKAC	3 cut(s)	616, 1254, 1450
AccII	CGCG	2 cut(s)	2842, 3311
AccIII	TCCGGA	2 cut(s)	1072, 4171
AclI	AACGTT	2 cut(s)	3550, 4178
AclWI	GGATC	6 cut(s)	527, 540, 640, 2646, 3608, 4191
AcoI	YGGCCR	3 cut(s)	678, 1882, 3680
AcsI	RAATTY	5 cut(s)	1203, 2053, 3076, 3418, 4218
AcuI	CTGAAG	4 cut(s)	1934, 3567, 3867, 4639
AcyI	GRCGYC	1 cut(s)	499
AfaI	GTAC	7 cut(s)	694, 1306, 1573, 2124, 2988, 3280, 4483
AfeI	AGCGCT	1 cut(s)	2572
AflII	CTTAAG	1 cut(s)	1784
AhlI	ACTAGT	1 cut(s)	4304
AjiI	CACGTC	1 cut(s)	464
AjuI	GAANNNNNNNTTGG	2 cut(s)	3409, 3441
AloI	GAACNNNNNNTCC	4 cut(s)	330, 362, 2175, 2207
Alw21I	GWGCWC	6 cut(s)	389, 1439, 1771, 2293, 4549, 4861
Alw44I	GTGCAC	1 cut(s)	1435
AlwI	GGATC	6 cut(s)	527, 540, 640, 2646, 3608, 4191
AlwNI	CAGNNNCTG	1 cut(s)	3890
Ama87I	CYCGRG	2 cut(s)	1977, 2413
Aor13HI	TCCGGA	2 cut(s)	1072, 4171
Aor51HI	AGCGCT	1 cut(s)	2572
ApaLI	GTGCAC	1 cut(s)	1435
ApeKI	GCWGC	5 cut(s)	396, 575, 761, 2543, 2954
ApoI	RAATTY	5 cut(s)	1203, 2053, 3076, 3418, 4218
ArsI	GACNNNNNNTTYG	4 cut(s)	149, 181, 4114, 4146
AseI	ATTAAT	2 cut(s)	1818, 3323
Asp700I	GAANNNNTTC	1 cut(s)	3699
AspA2I	CCTAGG	1 cut(s)	881
AspLEI	GCGC	2 cut(s)	2573, 2959
AsuC2I	CCSGG	5 cut(s)	328, 1124, 2909, 3245, 4904
AsuHPI	GGTGA	9 cut(s)	516, 652, 1151, 1361, 2012, 2137, 3184, 4291, 4825
AsuII	TTCGAA	1 cut(s)	195
AvaI	CYCGRG	2 cut(s)	1977, 2413
AvaII	GGWCC	7 cut(s)	145, 157, 1894, 2709, 2860, 3934, 4210
AvrII	CCTAGG	1 cut(s)	881
AxyI	CCTNAGG	1 cut(s)	3961
BaeGI	GKGCMC	4 cut(s)	53, 1375, 1439, 3227
BalI	TGGCCA	1 cut(s)	1884
BamHI	GGATCC	1 cut(s)	532
BanI	GGYRCC	3 cut(s)	50, 3222, 3294
BanII	GRGCYC	3 cut(s)	75, 997, 4861
BauI	CACGAG	2 cut(s)	1579, 4402
BbsI	GAAGAC	3 cut(s)	2488, 3473, 3642
Bbv12I	GWGCWC	6 cut(s)	389, 1439, 1771, 2293, 4549, 4861
BbvCI	CCTCAGC	1 cut(s)	2247
BbvI	GCAGC	5 cut(s)	408, 562, 773, 2530, 2966
BcgI	CGANNNNNNTGC	6 cut(s)	1435, 1469, 2525, 2559, 4868, 4902
BciVI	GTATCC	3 cut(s)	267, 1222, 4319
BclI	TGATCA	3 cut(s)	625, 2095, 3838
BcnI	CCSGG	5 cut(s)	328, 1124, 2909, 3245, 4904
BcuI	ACTAGT	1 cut(s)	4304
BfaI	CTAG	9 cut(s)	474, 486, 882, 1926, 1943, 2547, 3138, 3450, 4305
BfmI	CTRYAG	2 cut(s)	435, 5050
BfoI	RGCGCY	2 cut(s)	2574, 2960
BfrI	CTTAAG	1 cut(s)	1784
BfuAI	ACCTGC	1 cut(s)	848
BfuI	GTATCC	3 cut(s)	267, 1222, 4319
BglI	GCCNNNNNGGC	1 cut(s)	70
BglII	AGATCT	2 cut(s)	1841, 3366
BisI	GCNGC	8 cut(s)	397, 561, 576, 759, 762, 2544, 2955, 3309
BlnI	CCTAGG	1 cut(s)	881
BlpI	GCTNAGC	1 cut(s)	297
BlsI	GCNGC	8 cut(s)	398, 562, 577, 760, 763, 2545, 2956, 3310
BmcAI	AGTACT	1 cut(s)	3280
Bme18I	GGWCC	7 cut(s)	145, 157, 1894, 2709, 2860, 3934, 4210
BmeT110I	CYCGRG	2 cut(s)	1977, 2413
BmgBI	CACGTC	1 cut(s)	464
BmrI	ACTGGG	4 cut(s)	2955, 3203, 4033, 4097
BmuI	ACTGGG	4 cut(s)	2955, 3203, 4033, 4097
BpiI	GAAGAC	3 cut(s)	2488, 3473, 3642
BplI	GAGNNNNNCTC	2 cut(s)	830, 862
BpmI	CTGGAG	2 cut(s)	1822, 3941
Bpu10I	CCTNAGC	2 cut(s)	2247, 2562
Bpu1102I	GCTNAGC	1 cut(s)	297
Bpu14I	TTCGAA	1 cut(s)	195
BpuEI	CTTGAG	3 cut(s)	2033, 3080, 3710
BpuMI	CCSGG	5 cut(s)	328, 1124, 2909, 3245, 4904
Bsa29I	ATCGAT	2 cut(s)	2352, 2703
BsaAI	YACGTR	1 cut(s)	1766
BsaBI	GATNNNNATC	1 cut(s)	2348
BsaHI	GRCGYC	1 cut(s)	499
BsaI	GGTCTC	4 cut(s)	99, 436, 3429, 3728
BsaWI	WCCGGW	3 cut(s)	1072, 1653, 4171
BsaXI	ACNNNNNCTCC	2 cut(s)	407, 437
Bse118I	RCCGGY	2 cut(s)	676, 2818
Bse21I	CCTNAGG	1 cut(s)	3961
Bse3DI	GCAATG	2 cut(s)	2475, 4936
Bse8I	GATNNNNATC	1 cut(s)	2348
BseAI	TCCGGA	2 cut(s)	1072, 4171
BseCI	ATCGAT	2 cut(s)	2352, 2703
BseJI	GATNNNNATC	1 cut(s)	2348
BseMI	GCAATG	2 cut(s)	2475, 4936
BseMII	CTCAG	9 cut(s)	311, 540, 1815, 1881, 2238, 2553, 3921, 3975, 4470
BseRI	GAGGAG	6 cut(s)	65, 508, 700, 703, 772, 4575
BseSI	GKGCMC	4 cut(s)	53, 1375, 1439, 3227
BseXI	GCAGC	5 cut(s)	408, 562, 773, 2530, 2966
BseYI	CCCAGC	3 cut(s)	2334, 3555, 3846
BsgI	GTGCAG	2 cut(s)	1179, 1880
Bsh1236I	CGCG	2 cut(s)	2842, 3311
Bsh1285I	CGRYCG	1 cut(s)	1519
BshNI	GGYRCC	3 cut(s)	50, 3222, 3294
BshVI	ATCGAT	2 cut(s)	2352, 2703
BsiEI	CGRYCG	1 cut(s)	1519
BsiHKAI	GWGCWC	6 cut(s)	389, 1439, 1771, 2293, 4549, 4861
BsiHKCI	CYCGRG	2 cut(s)	1977, 2413
BslFI	GGGAC	8 cut(s)	170, 1140, 2873, 2965, 3191, 4085, 4673, 4691
BsmBI	CGTCTC	2 cut(s)	490, 925
BsmFI	GGGAC	8 cut(s)	170, 1140, 2873, 2965, 3191, 4085, 4673, 4691
BsmI	GAATGC	4 cut(s)	723, 2627, 2782, 3897
Bso31I	GGTCTC	4 cut(s)	99, 436, 3429, 3728
BsoBI	CYCGRG	2 cut(s)	1977, 2413
Bsp119I	TTCGAA	1 cut(s)	195
Bsp13I	TCCGGA	2 cut(s)	1072, 4171
Bsp1720I	GCTNAGC	1 cut(s)	297
Bsp19I	CCATGG	2 cut(s)	1005, 2274
Bsp68I	TCGCGA	1 cut(s)	2842
BspCNI	CTCAG	9 cut(s)	310, 541, 1814, 1882, 2239, 2554, 3922, 3974, 4469
BspDI	ATCGAT	2 cut(s)	2352, 2703
BspEI	TCCGGA	2 cut(s)	1072, 4171
BspFNI	CGCG	2 cut(s)	2842, 3311
BspHI	TCATGA	4 cut(s)	2098, 3901, 4090, 4621
BspMI	ACCTGC	1 cut(s)	848
BspPI	GGATC	6 cut(s)	527, 540, 640, 2646, 3608, 4191
BspQI	GCTCTTC	2 cut(s)	1953, 2241
BspT104I	TTCGAA	1 cut(s)	195
BspT107I	GGYRCC	3 cut(s)	50, 3222, 3294
BspTI	CTTAAG	1 cut(s)	1784
BspTNI	GGTCTC	4 cut(s)	99, 436, 3429, 3728
BsrBI	CCGCTC	3 cut(s)	252, 563, 3384
BsrDI	GCAATG	2 cut(s)	2475, 4936
BsrFI	RCCGGY	2 cut(s)	676, 2818
BssAI	RCCGGY	2 cut(s)	676, 2818
BssNAI	GTATAC	1 cut(s)	617
BssNI	GRCGYC	1 cut(s)	499
BssSI	CACGAG	2 cut(s)	1579, 4402
Bst1107I	GTATAC	1 cut(s)	617
Bst2BI	CACGAG	2 cut(s)	1579, 4402
Bst6I	CTCTTC	9 cut(s)	513, 882, 1321, 1682, 1953, 2073, 2241, 2889, 4679
BstACI	GRCGYC	1 cut(s)	499
BstAFI	CTTAAG	1 cut(s)	1784
BstAPI	GCANNNNNTGC	2 cut(s)	1443, 2117
BstBAI	YACGTR	1 cut(s)	1766
BstBI	TTCGAA	1 cut(s)	195
BstC8I	GCNNGC	8 cut(s)	218, 678, 822, 1276, 1408, 1756, 2824, 2840
BstDSI	CCRYGG	3 cut(s)	528, 1005, 2274
BstEII	GGTNACC	1 cut(s)	2000
BstENI	CCTNNNNNAGG	3 cut(s)	548, 3302, 4676
BstFNI	CGCG	2 cut(s)	2842, 3311
BstH2I	RGCGCY	2 cut(s)	2574, 2960
BstHHI	GCGC	2 cut(s)	2573, 2959
BstMCI	CGRYCG	1 cut(s)	1519
BstNSI	RCATGY	5 cut(s)	220, 820, 1435, 1669, 1758
BstPI	GGTNACC	1 cut(s)	2000
BstSFI	CTRYAG	2 cut(s)	435, 5050
BstSLI	GKGCMC	4 cut(s)	53, 1375, 1439, 3227
BstUI	CGCG	2 cut(s)	2842, 3311
BstV1I	GCAGC	5 cut(s)	408, 562, 773, 2530, 2966
BstV2I	GAAGAC	3 cut(s)	2488, 3473, 3642
BstX2I	RGATCY	3 cut(s)	532, 1841, 3366
BstXI	CCANNNNNNTGG	2 cut(s)	4319, 4569
BstYI	RGATCY	3 cut(s)	532, 1841, 3366
BstZ17I	GTATAC	1 cut(s)	617
Bsu15I	ATCGAT	2 cut(s)	2352, 2703
Bsu36I	CCTNAGG	1 cut(s)	3961
BsuI	GTATCC	3 cut(s)	267, 1222, 4319
BsuTUI	ATCGAT	2 cut(s)	2352, 2703
BtgI	CCRYGG	3 cut(s)	528, 1005, 2274
BtgZI	GCGATG	2 cut(s)	2069, 3827
BtrI	CACGTC	1 cut(s)	464
BtsI	GCAGTG	4 cut(s)	1597, 2229, 4205, 4743
BtuMI	TCGCGA	1 cut(s)	2842
BveI	ACCTGC	1 cut(s)	848
Cac8I	GCNNGC	8 cut(s)	218, 678, 822, 1276, 1408, 1756, 2824, 2840
CaiI	CAGNNNCTG	1 cut(s)	3890
CciI	TCATGA	4 cut(s)	2098, 3901, 4090, 4621
CfoI	GCGC	2 cut(s)	2573, 2959
Cfr10I	RCCGGY	2 cut(s)	676, 2818
ClaI	ATCGAT	2 cut(s)	2352, 2703
CseI	GACGC	1 cut(s)	507
Csp6I	GTAC	7 cut(s)	693, 1305, 1572, 2123, 2987, 3279, 4482
CspCI	CAANNNNNGTGG	2 cut(s)	775, 810
CviQI	GTAC	7 cut(s)	693, 1305, 1572, 2123, 2987, 3279, 4482
DraI	TTTAAA	6 cut(s)	957, 1660, 1902, 2670, 3444, 4536
EaeI	YGGCCR	3 cut(s)	678, 1882, 3680
Eam1104I	CTCTTC	9 cut(s)	513, 882, 1321, 1682, 1953, 2073, 2241, 2889, 4679
EarI	CTCTTC	9 cut(s)	513, 882, 1321, 1682, 1953, 2073, 2241, 2889, 4679
EciI	GGCGGA	4 cut(s)	630, 657, 2030, 2465
Ecl136II	GAGCTC	1 cut(s)	4859
Eco24I	GRGCYC	3 cut(s)	75, 997, 4861
Eco31I	GGTCTC	4 cut(s)	99, 436, 3429, 3728
Eco32I	GATATC	2 cut(s)	1168, 1746
Eco47I	GGWCC	7 cut(s)	145, 157, 1894, 2709, 2860, 3934, 4210
Eco47III	AGCGCT	1 cut(s)	2572
Eco53kI	GAGCTC	1 cut(s)	4859
Eco57I	CTGAAG	4 cut(s)	1934, 3567, 3867, 4639
Eco81I	CCTNAGG	1 cut(s)	3961
Eco88I	CYCGRG	2 cut(s)	1977, 2413
Eco91I	GGTNACC	1 cut(s)	2000
EcoICRI	GAGCTC	1 cut(s)	4859
EcoNI	CCTNNNNNAGG	3 cut(s)	548, 3302, 4676
EcoO109I	RGGNCCY	5 cut(s)	157, 539, 1331, 1918, 2860
EcoO65I	GGTNACC	1 cut(s)	2000
EcoRI	GAATTC	2 cut(s)	2053, 3076
EcoRV	GATATC	2 cut(s)	1168, 1746
EcoT22I	ATGCAT	2 cut(s)	1418, 2627
EcoT38I	GRGCYC	3 cut(s)	75, 997, 4861
Esp3I	CGTCTC	2 cut(s)	490, 925
FalI	AAGNNNNNCTT	4 cut(s)	858, 890, 3719, 3751
FaqI	GGGAC	8 cut(s)	170, 1140, 2873, 2965, 3191, 4085, 4673, 4691
FauI	CCCGC	2 cut(s)	1404, 3389
FauNDI	CATATG	2 cut(s)	4784, 4836
FbaI	TGATCA	3 cut(s)	625, 2095, 3838
FblI	GTMKAC	3 cut(s)	616, 1254, 1450
Fnu4HI	GCNGC	8 cut(s)	397, 561, 576, 759, 762, 2544, 2955, 3309
FriOI	GRGCYC	3 cut(s)	75, 997, 4861
Fsp4HI	GCNGC	8 cut(s)	397, 561, 576, 759, 762, 2544, 2955, 3309
FspBI	CTAG	9 cut(s)	474, 486, 882, 1926, 1943, 2547, 3138, 3450, 4305
GlaI	GCGC	2 cut(s)	2572, 2958
GluI	GCNGC	8 cut(s)	397, 561, 576, 759, 762, 2544, 2955, 3309
GsaI	CCCAGC	3 cut(s)	2338, 3559, 3850
GsuI	CTGGAG	2 cut(s)	1822, 3941
HaeII	RGCGCY	2 cut(s)	2574, 2960
HgaI	GACGC	1 cut(s)	507
HhaI	GCGC	2 cut(s)	2573, 2959
Hin1I	GRCGYC	1 cut(s)	499
Hin6I	GCGC	2 cut(s)	2571, 2957
HinP1I	GCGC	2 cut(s)	2571, 2957
HindIII	AAGCTT	5 cut(s)	2374, 2672, 2688, 4432, 4468
HpaI	GTTAAC	2 cut(s)	2167, 4021
HphI	GGTGA	9 cut(s)	516, 652, 1151, 1361, 2012, 2137, 3184, 4291, 4825
Hpy99I	CGWCG	2 cut(s)	465, 2216
HpyCH4IV	ACGT	6 cut(s)	463, 1765, 2587, 3550, 4033, 4178
HpySE526I	ACGT	6 cut(s)	463, 1765, 2587, 3550, 4033, 4178
Hsp92I	GRCGYC	1 cut(s)	499
HspAI	GCGC	2 cut(s)	2571, 2957
KflI	GGGWCCC	1 cut(s)	2860
Kpn2I	TCCGGA	2 cut(s)	1072, 4171
KroI	GCCGGC	1 cut(s)	676
KroNI	GCCGGC	1 cut(s)	678
Ksp22I	TGATCA	3 cut(s)	625, 2095, 3838
KspAI	GTTAAC	2 cut(s)	2167, 4021
LguI	GCTCTTC	2 cut(s)	1953, 2241
LmnI	GCTCC	8 cut(s)	9, 78, 392, 568, 673, 2296, 4552, 4682
Lsp1109I	GCAGC	5 cut(s)	408, 562, 773, 2530, 2966
MaeI	CTAG	9 cut(s)	474, 486, 882, 1926, 1943, 2547, 3138, 3450, 4305
MaeII	ACGT	6 cut(s)	463, 1765, 2587, 3550, 4033, 4178
MbiI	CCGCTC	3 cut(s)	252, 563, 3384
MfeI	CAATTG	4 cut(s)	1308, 2592, 3748, 4771
MflI	RGATCY	3 cut(s)	532, 1841, 3366
MlsI	TGGCCA	1 cut(s)	1884
MluNI	TGGCCA	1 cut(s)	1884
MlyI	GAGTC	9 cut(s)	377, 666, 2594, 2741, 3052, 3679, 4448, 4749, 4771
MmeI	TCCRAC	5 cut(s)	1845, 3178, 3389, 4161, 4336
Mox20I	TGGCCA	1 cut(s)	1884
Mph1103I	ATGCAT	2 cut(s)	1418, 2627
MroI	TCCGGA	2 cut(s)	1072, 4171
MroNI	GCCGGC	1 cut(s)	676
MroXI	GAANNNNTTC	1 cut(s)	3699
MscI	TGGCCA	1 cut(s)	1884
MslI	CAYNNNNRTG	3 cut(s)	610, 2620, 3810
Msp20I	TGGCCA	1 cut(s)	1884
MspCI	CTTAAG	1 cut(s)	1784
MssI	GTTTAAAC	2 cut(s)	1660, 1902
MunI	CAATTG	4 cut(s)	1308, 2592, 3748, 4771
Mva1269I	GAATGC	4 cut(s)	723, 2627, 2782, 3897
MvnI	CGCG	2 cut(s)	2842, 3311
NaeI	GCCGGC	1 cut(s)	678
NciI	CCSGG	5 cut(s)	328, 1124, 2909, 3245, 4904
NcoI	CCATGG	2 cut(s)	1005, 2274
NdeI	CATATG	2 cut(s)	4784, 4836
NgoMIV	GCCGGC	1 cut(s)	676
NruI	TCGCGA	1 cut(s)	2842
NsiI	ATGCAT	2 cut(s)	1418, 2627
NspI	RCATGY	5 cut(s)	220, 820, 1435, 1669, 1758
NspV	TTCGAA	1 cut(s)	195
PaeI	GCATGC	2 cut(s)	220, 1758
PaeR7I	CTCGAG	2 cut(s)	1977, 2413
PagI	TCATGA	4 cut(s)	2098, 3901, 4090, 4621
PaqCI	CACCTGC	1 cut(s)	848
PciSI	GCTCTTC	2 cut(s)	1953, 2241
PcsI	WCGNNNNNNNCGW	4 cut(s)	1516, 3130, 4740, 4951
PctI	GAATGC	4 cut(s)	723, 2627, 2782, 3897
PdiI	GCCGGC	1 cut(s)	678
PdmI	GAANNNNTTC	1 cut(s)	3699
PfeI	GAWTC	6 cut(s)	478, 521, 748, 1547, 4799, 5055
PflFI	GACNNNGTC	2 cut(s)	328, 3938
PflMI	CCANNNNNTGG	2 cut(s)	1146, 2706
PkrI	GCNGC	8 cut(s)	398, 562, 577, 760, 763, 2545, 2956, 3310
Ple19I	CGATCG	1 cut(s)	1519
PleI	GAGTC	9 cut(s)	376, 665, 2594, 2741, 3051, 3679, 4448, 4749, 4771
PmeI	GTTTAAAC	2 cut(s)	1660, 1902
PpsI	GAGTC	9 cut(s)	376, 665, 2594, 2741, 3051, 3679, 4448, 4749, 4771
Ppu21I	YACGTR	1 cut(s)	1766
PpuMI	RGGWCCY	2 cut(s)	157, 2860
PshBI	ATTAAT	2 cut(s)	1818, 3323
PsiI	TTATAA	1 cut(s)	2579
Psp124BI	GAGCTC	1 cut(s)	4861
Psp1406I	AACGTT	2 cut(s)	3550, 4178
Psp5II	RGGWCCY	2 cut(s)	157, 2860
PspEI	GGTNACC	1 cut(s)	2000
PspFI	CCCAGC	3 cut(s)	2334, 3555, 3846
PspPPI	RGGWCCY	2 cut(s)	157, 2860
PspXI	VCTCGAGB	2 cut(s)	1977, 2413
PstNI	CAGNNNCTG	1 cut(s)	3890
PsuI	RGATCY	3 cut(s)	532, 1841, 3366
PsyI	GACNNNGTC	2 cut(s)	328, 3938
PvuI	CGATCG	1 cut(s)	1519
RruI	TCGCGA	1 cut(s)	2842
RsaI	GTAC	7 cut(s)	694, 1306, 1573, 2124, 2988, 3280, 4483
RsaNI	GTAC	7 cut(s)	693, 1305, 1572, 2123, 2987, 3279, 4482
RseI	CAYNNNNRTG	3 cut(s)	610, 2620, 3810
SacI	GAGCTC	1 cut(s)	4861
SapI	GCTCTTC	2 cut(s)	1953, 2241
SatI	GCNGC	8 cut(s)	397, 561, 576, 759, 762, 2544, 2955, 3309
ScaI	AGTACT	1 cut(s)	3280
SchI	GAGTC	9 cut(s)	377, 666, 2594, 2741, 3052, 3679, 4448, 4749, 4771
SfcI	CTRYAG	2 cut(s)	435, 5050
Sfr274I	CTCGAG	2 cut(s)	1977, 2413
SfuI	TTCGAA	1 cut(s)	195
SinI	GGWCC	7 cut(s)	145, 157, 1894, 2709, 2860, 3934, 4210
SlaI	CTCGAG	2 cut(s)	1977, 2413
SmiMI	CAYNNNNRTG	3 cut(s)	610, 2620, 3810
SmlI	CTYRAG	6 cut(s)	1784, 1977, 2048, 2413, 3059, 3725
SmoI	CTYRAG	6 cut(s)	1784, 1977, 2048, 2413, 3059, 3725
SpeI	ACTAGT	1 cut(s)	4304
SphI	GCATGC	2 cut(s)	220, 1758
SspI	AATATT	3 cut(s)	979, 2662, 3784
SspMI	CTAG	9 cut(s)	474, 486, 882, 1926, 1943, 2547, 3138, 3450, 4305
SstI	GAGCTC	1 cut(s)	4861
TaiI	ACGT	6 cut(s)	466, 1768, 2590, 3553, 4036, 4181
TatI	WGTACW	3 cut(s)	1304, 2986, 3278
TauI	GCSGC	3 cut(s)	563, 761, 3311
TfiI	GAWTC	6 cut(s)	478, 521, 748, 1547, 4799, 5055
TseI	GCWGC	5 cut(s)	396, 575, 761, 2543, 2954
TspGWI	ACGGA	5 cut(s)	351, 505, 545, 3055, 4811
Tth111I	GACNNNGTC	2 cut(s)	328, 3938
Van91I	CCANNNNNTGG	2 cut(s)	1146, 2706
Vha464I	CTTAAG	1 cut(s)	1784
VneI	GTGCAC	1 cut(s)	1435
VpaK11BI	GGWCC	7 cut(s)	145, 157, 1894, 2709, 2860, 3934, 4210
VspI	ATTAAT	2 cut(s)	1818, 3323
XagI	CCTNNNNNAGG	3 cut(s)	548, 3302, 4676
XapI	RAATTY	5 cut(s)	1203, 2053, 3076, 3418, 4218
XceI	RCATGY	5 cut(s)	220, 820, 1435, 1669, 1758
XhoI	CTCGAG	2 cut(s)	1977, 2413
XmaJI	CCTAGG	1 cut(s)	881
XmiI	GTMKAC	3 cut(s)	616, 1254, 1450
XmnI	GAANNNNTTC	1 cut(s)	3699
XspI	CTAG	9 cut(s)	474, 486, 882, 1926, 1943, 2547, 3138, 3450, 4305
ZrmI	AGTACT	1 cut(s)	3280
Zsp2I	ATGCAT	2 cut(s)	1418, 2627

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.