Rorug06G0057600

ubiquitin-like-specific protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0002136
Physical Location & Seq
Reverse (-)
7788710 .. 7792768
4059 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0057600.1

Sequence Viewer

Length: 1353 bp
ATGAGTAGTCGTCGTGGGTTTTCGAACATGAGAGCAAAAGGTAGTCTTCATCCTCCAAATGCCATGAGGAAAAGAGGCAAACAAAAAGCTGGAGGAAGAACTGTTGCTGATGAGTATGAGGATGAGTTACCGGAAAAACTGACTCAGGTTCCTCATCCCTGCCCCTCGGGGCCAATTTCTACTTACTTAGTAGAAGGGTTATATGAGAAAAGGGGAGATAAGCGAGAGGAGACACTAACCTTTATGATTGAAGGCTTAAATAGAAACCTAAGCAAAAACTTGCTGGAAGAGAAGTTTGCTACCTTTCTATACAGATGCTTGAACTCTGTGAAGAAAGGTTCTACCAAGGAGGTGAATAATTCATTTCATGTGATAGGCTTGCTGGCAACAATAGTTGGTTGTGAAGATAAACTCTCTGAAATATATAGAGAGTTGCTTCCTGTACTTTCCGGAGCTCTGAAATCTGGGTCCACAACAATAAAGATATTGGATTGCTTGGCTATTGTTGCTTTCTTTGGTGCAACCAATCCAGATGAAACAGAGAGTGCCATGCGTATTATATGGGAGTACCTCAATCCCGAGTCGGGTTATGATGTCAACACAAAAAATAATTCAGCGGAGTTACTAGTTGCCGCAGTATATGCTTGGTTGTTTCTTCTTTCAAGCATGGAGGGATTTAGACTCAGCTACACCTACTGGAAAGGGGCCATTTCATACTTCGCAAATTTACTAGATCACGACAACAAATTAGTATGTGGAGCAGCTAGTGAAGCATTAGCTTTAATTTTTGAAACTGGCTCTCTTGAAAAGTTCTGGAATGAAGAAAATGGTTCAAGCCCCAGTTATTCACAAATGCAACAATCACTTGGAGCCCATCTTTTAAAGAAAGTTAAATGTGCTTACAAAAATCTACAAACTGAGAATAATGCAGACAAAATTCGCAATGTTGTAAGATATTTTGAGAGTTCTCATGTCCTGGCAGAATCTTTAGTGGTCAAAGGGAAAGTACTAACGTTGTCATCATGGTATCAAATAATTCAGCTGCAGTTTTTGAAGAGGTTTCTAGCTGATGGATTTAAAAAACATATGGAGGAAAATGAGAAGCTTCAGAATTTGTTCGAGTTCGATCCACGTAAAAGCAAGAATTTAGGTCCAGAGTTGTATGTGGCTACTACTGATAAGATCTATGTGCGCTTCTTTATGCCTGAAGAAAGAGATCAATACATGCTAACGAAGGAACAAAGAAAGAAAGACAGGCTTCGGAGGAGCTCCATTGTAGACAAAGCCAAGACACAATTGATGACAAAACATCGTTTGTTGTCGAAGGAACTAAAAAGCTGCGACTATGATTGA

Protein Analysis

450

Amino Acids

51.38

Weight (kDa)

8.94

Isoelectric Point (pI)

35.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IFRD PF05004 64 - 298 1.2e-42 Interferon-related developmental regulator (IFRD)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1278
AccIII TCCGGA 1 cut(s) 449
AciI CCGC 2 cut(s) 617, 633
AclI AACGTT 1 cut(s) 1013
AclWI GGATC 1 cut(s) 1121
AcsI RAATTY 4 cut(s) 724, 936, 1111, 1144
AcuI CTGAAG 2 cut(s) 1091, 1227
AfaI GTAC 3 cut(s) 444, 569, 1008
AfiI CCNNNNNNNGG 1 cut(s) 584
AgsI TTSAA 7 cut(s) 251, 322, 663, 791, 806, 834, 1054
AhlI ACTAGT 1 cut(s) 625
AjnI CCWGG 1 cut(s) 975
Alw21I GWGCWC 2 cut(s) 457, 1271
Alw26I GTCTC 1 cut(s) 224
AlwI GGATC 1 cut(s) 1121
Ama87I CYCGRG 2 cut(s) 166, 578
Aor13HI TCCGGA 1 cut(s) 449
AoxI GGCC 2 cut(s) 170, 705
ApeKI GCWGC 3 cut(s) 761, 1042, 1338
ApoI RAATTY 4 cut(s) 724, 936, 1111, 1144
Asp700I GAANNNNTTC 1 cut(s) 1115
AspLEI GCGC 1 cut(s) 1194
AspS9I GGNCC 4 cut(s) 170, 468, 705, 1151
AsuHPI GGTGA 1 cut(s) 364
AsuII TTCGAA 1 cut(s) 23
AvaI CYCGRG 2 cut(s) 166, 578
AvaII GGWCC 2 cut(s) 468, 1151
BanII GRGCYC 3 cut(s) 457, 874, 1271
BbsI GAAGAC 1 cut(s) 38
Bbv12I GWGCWC 2 cut(s) 457, 1271
BbvI GCAGC 3 cut(s) 773, 1029, 1325
BccI CCATC 2 cut(s) 882, 1064
BciT130I CCWGG 1 cut(s) 977
BcoDI GTCTC 1 cut(s) 224
BcuI ACTAGT 1 cut(s) 625
BfaI CTAG 4 cut(s) 626, 731, 765, 1064
BfmI CTRYAG 1 cut(s) 1043
BglII AGATCT 1 cut(s) 1182
BisI GCNGC 4 cut(s) 633, 762, 1043, 1339
BlsI GCNGC 4 cut(s) 634, 763, 1044, 1340
BmcAI AGTACT 1 cut(s) 1008
Bme1390I CCNGG 1 cut(s) 977
Bme18I GGWCC 2 cut(s) 468, 1151
BmeT110I CYCGRG 2 cut(s) 166, 578
BmgT120I GGNCC 4 cut(s) 170, 468, 705, 1151
BmiI GGNNCC 5 cut(s) 150, 171, 469, 706, 871
BmrFI CCNGG 1 cut(s) 977
BmrI ACTGGG 1 cut(s) 834
BmsI GCATC 1 cut(s) 305
BmuI ACTGGG 1 cut(s) 834
BpiI GAAGAC 1 cut(s) 38
BpmI CTGGAG 1 cut(s) 111
Bpu10I CCTNAGC 1 cut(s) 269
Bpu14I TTCGAA 1 cut(s) 23
BsaAI YACGTR 1 cut(s) 1133
BsaJI CCNNGG 2 cut(s) 165, 345
BsaWI WCCGGW 2 cut(s) 130, 449
Bsc4I CCNNNNNNNGG 1 cut(s) 584
Bse1I ACTGG 3 cut(s) 701, 799, 840
Bse3DI GCAATG 1 cut(s) 949
BseAI TCCGGA 1 cut(s) 449
BseBI CCWGG 1 cut(s) 977
BseDI CCNNGG 2 cut(s) 165, 345
BseGI GGATG 3 cut(s) 49, 127, 154
BseLI CCNNNNNNNGG 1 cut(s) 584
BseMI GCAATG 1 cut(s) 949
BseMII CTCAG 3 cut(s) 158, 697, 909
BseNI ACTGG 3 cut(s) 701, 799, 840
BseRI GAGGAG 2 cut(s) 242, 1279
BseXI GCAGC 3 cut(s) 773, 1029, 1325
BshFI GGCC 2 cut(s) 172, 707
BsiHKAI GWGCWC 2 cut(s) 457, 1271
BsiHKCI CYCGRG 2 cut(s) 166, 578
BsiSI CCGG 2 cut(s) 131, 450
BslI CCNNNNNNNGG 1 cut(s) 584
BsmAI GTCTC 1 cut(s) 224
BsnI GGCC 2 cut(s) 172, 707
BsoBI CYCGRG 2 cut(s) 166, 578
Bsp119I TTCGAA 1 cut(s) 23
Bsp1286I GDGCHC 3 cut(s) 457, 874, 1271
Bsp13I TCCGGA 1 cut(s) 449
Bsp143I GATC 4 cut(s) 733, 1126, 1182, 1216
BspACI CCGC 2 cut(s) 617, 633
BspANI GGCC 2 cut(s) 172, 707
BspCNI CTCAG 3 cut(s) 157, 696, 910
BspEI TCCGGA 1 cut(s) 449
BspLI GGNNCC 5 cut(s) 150, 171, 469, 706, 871
BspMAI CTGCAG 1 cut(s) 1047
BspPI GGATC 1 cut(s) 1121
BspT104I TTCGAA 1 cut(s) 23
BsrDI GCAATG 1 cut(s) 949
BsrI ACTGG 3 cut(s) 701, 799, 840
BssECI CCNNGG 2 cut(s) 165, 345
BssMI GATC 4 cut(s) 733, 1126, 1182, 1216
BssT1I CCWWGG 1 cut(s) 345
Bst2UI CCWGG 1 cut(s) 977
Bst4CI ACNGT 1 cut(s) 103
Bst6I CTCTTC 2 cut(s) 282, 1049
BstAPI GCANNNNNTGC 1 cut(s) 641
BstBAI YACGTR 1 cut(s) 1133
BstBI TTCGAA 1 cut(s) 23
BstC8I GCNNGC 2 cut(s) 380, 384
BstDEI CTNAG 5 cut(s) 144, 187, 269, 683, 918
BstF5I GGATG 3 cut(s) 49, 127, 154
BstHHI GCGC 1 cut(s) 1194
BstKTI GATC 4 cut(s) 736, 1129, 1185, 1219
BstMAI GTCTC 1 cut(s) 224
BstMBI GATC 4 cut(s) 733, 1126, 1182, 1216
BstMWI GCNNNNNNNGC 3 cut(s) 506, 641, 770
BstNI CCWGG 1 cut(s) 977
BstNSI RCATGY 1 cut(s) 1228
BstSCI CCNGG 1 cut(s) 975
BstSFI CTRYAG 1 cut(s) 1043
BstV1I GCAGC 3 cut(s) 773, 1029, 1325
BstV2I GAAGAC 1 cut(s) 38
BstX2I RGATCY 1 cut(s) 1182
BstYI RGATCY 1 cut(s) 1182
BsuRI GGCC 2 cut(s) 172, 707
BtsCI GGATG 3 cut(s) 49, 127, 154
Cac8I GCNNGC 2 cut(s) 380, 384
CfoI GCGC 1 cut(s) 1194
Cfr13I GGNCC 4 cut(s) 170, 468, 705, 1151
Csp6I GTAC 3 cut(s) 443, 568, 1007
CviAII CATG 8 cut(s) 28, 64, 368, 550, 667, 971, 1023, 1225
CviQI GTAC 3 cut(s) 443, 568, 1007
DdeI CTNAG 5 cut(s) 144, 187, 269, 683, 918
DpnI GATC 4 cut(s) 735, 1128, 1184, 1218
DpnII GATC 4 cut(s) 733, 1126, 1182, 1216
DraI TTTAAA 2 cut(s) 882, 1078
Eam1104I CTCTTC 2 cut(s) 282, 1049
EarI CTCTTC 2 cut(s) 282, 1049
Ecl136II GAGCTC 2 cut(s) 455, 1269
Eco130I CCWWGG 1 cut(s) 345
Eco24I GRGCYC 3 cut(s) 457, 874, 1271
Eco47I GGWCC 2 cut(s) 468, 1151
Eco53kI GAGCTC 2 cut(s) 455, 1269
Eco57I CTGAAG 2 cut(s) 1091, 1227
Eco88I CYCGRG 2 cut(s) 166, 578
EcoICRI GAGCTC 2 cut(s) 455, 1269
EcoRII CCWGG 1 cut(s) 975
EcoT14I CCWWGG 1 cut(s) 345
EcoT38I GRGCYC 3 cut(s) 457, 874, 1271
ErhI CCWWGG 1 cut(s) 345
FaeI CATG 8 cut(s) 31, 67, 371, 553, 670, 974, 1026, 1228
FalI AAGNNNNNCTT 4 cut(s) 30, 62, 1242, 1274
FatI CATG 8 cut(s) 27, 63, 367, 549, 666, 970, 1022, 1224
FauNDI CATATG 1 cut(s) 1086
FblI GTMKAC 1 cut(s) 1278
Fnu4HI GCNGC 4 cut(s) 633, 762, 1043, 1339
FokI GGATG 3 cut(s) 36, 134, 141
FriOI GRGCYC 3 cut(s) 457, 874, 1271
Fsp4HI GCNGC 4 cut(s) 633, 762, 1043, 1339
FspBI CTAG 4 cut(s) 626, 731, 765, 1064
GlaI GCGC 1 cut(s) 1193
GluI GCNGC 4 cut(s) 633, 762, 1043, 1339
GsuI CTGGAG 1 cut(s) 111
HaeIII GGCC 2 cut(s) 172, 707
HapII CCGG 2 cut(s) 131, 450
HhaI GCGC 1 cut(s) 1194
Hin1II CATG 8 cut(s) 31, 67, 371, 553, 670, 974, 1026, 1228
Hin6I GCGC 1 cut(s) 1192
HinP1I GCGC 1 cut(s) 1192
HincII GTYRAC 1 cut(s) 598
HindII GTYRAC 1 cut(s) 598
HindIII AAGCTT 1 cut(s) 1103
HinfI GANTC 4 cut(s) 142, 581, 681, 983
HpaII CCGG 2 cut(s) 131, 450
HphI GGTGA 1 cut(s) 364
Hpy166II GTNNAC 3 cut(s) 471, 598, 1279
Hpy188I TCNGA 4 cut(s) 418, 459, 1110, 1263
Hpy188III TCNNGA 7 cut(s) 450, 530, 578, 737, 803, 814, 1154
Hpy8I GTNNAC 3 cut(s) 471, 598, 1279
Hpy99I CGWCG 1 cut(s) 15
HpyAV CCTTC 4 cut(s) 188, 245, 1228, 1318
HpyCH4III ACNGT 1 cut(s) 103
HpyCH4IV ACGT 2 cut(s) 1013, 1132
HpyCH4V TGCA 4 cut(s) 521, 856, 929, 1045
HpyF10VI GCNNNNNNNGC 3 cut(s) 506, 641, 770
HpyF3I CTNAG 5 cut(s) 144, 187, 269, 683, 918
HpySE526I ACGT 2 cut(s) 1013, 1132
Hsp92II CATG 8 cut(s) 31, 67, 371, 553, 670, 974, 1026, 1228
HspAI GCGC 1 cut(s) 1192
Kpn2I TCCGGA 1 cut(s) 449
Kzo9I GATC 4 cut(s) 733, 1126, 1182, 1216
LmnI GCTCC 5 cut(s) 452, 758, 869, 1266, 1274
Lsp1109I GCAGC 3 cut(s) 773, 1029, 1325
LweI GCATC 1 cut(s) 305
MaeI CTAG 4 cut(s) 626, 731, 765, 1064
MaeII ACGT 2 cut(s) 1013, 1132
MaeIII GTNAC 2 cut(s) 126, 621
MalI GATC 4 cut(s) 735, 1128, 1184, 1218
MboI GATC 4 cut(s) 733, 1126, 1182, 1216
MboII GAAGA 9 cut(s) 38, 108, 299, 343, 416, 647, 833, 1066, 1220
MfeI CAATTG 1 cut(s) 1295
MflI RGATCY 1 cut(s) 1182
MhlI GDGCHC 3 cut(s) 457, 874, 1271
MlyI GAGTC 3 cut(s) 136, 590, 675
MroI TCCGGA 1 cut(s) 449
MroXI GAANNNNTTC 1 cut(s) 1115
MseI TTAA 5 cut(s) 257, 782, 881, 891, 1077
MspA1I CMGCKG 2 cut(s) 617, 1042
MspI CCGG 2 cut(s) 131, 450
MspR9I CCNGG 1 cut(s) 977
MunI CAATTG 1 cut(s) 1295
MvaI CCWGG 1 cut(s) 977
MwoI GCNNNNNNNGC 3 cut(s) 506, 641, 770
NdeI CATATG 1 cut(s) 1086
NdeII GATC 4 cut(s) 733, 1126, 1182, 1216
NlaIII CATG 8 cut(s) 31, 67, 371, 553, 670, 974, 1026, 1228
NlaIV GGNNCC 5 cut(s) 150, 171, 469, 706, 871
NspI RCATGY 1 cut(s) 1228
NspV TTCGAA 1 cut(s) 23
PdmI GAANNNNTTC 1 cut(s) 1115
PfeI GAWTC 1 cut(s) 983
PkrI GCNGC 4 cut(s) 634, 763, 1044, 1340
PleI GAGTC 3 cut(s) 136, 589, 675
PpsI GAGTC 3 cut(s) 136, 589, 675
Ppu21I YACGTR 1 cut(s) 1133
Psp124BI GAGCTC 2 cut(s) 457, 1271
Psp1406I AACGTT 1 cut(s) 1013
Psp6I CCWGG 1 cut(s) 975
PspGI CCWGG 1 cut(s) 975
PspN4I GGNNCC 5 cut(s) 150, 171, 469, 706, 871
PspPI GGNCC 4 cut(s) 170, 468, 705, 1151
PstI CTGCAG 1 cut(s) 1047
PsuI RGATCY 1 cut(s) 1182
PvuII CAGCTG 1 cut(s) 1042
RsaI GTAC 3 cut(s) 444, 569, 1008
RsaNI GTAC 3 cut(s) 443, 568, 1007
SacI GAGCTC 2 cut(s) 457, 1271
SaqAI TTAA 5 cut(s) 257, 782, 881, 891, 1077
SatI GCNGC 4 cut(s) 633, 762, 1043, 1339
Sau3AI GATC 4 cut(s) 733, 1126, 1182, 1216
Sau96I GGNCC 4 cut(s) 170, 468, 705, 1151
ScaI AGTACT 1 cut(s) 1008
SchI GAGTC 3 cut(s) 136, 590, 675
ScrFI CCNGG 1 cut(s) 977
SduI GDGCHC 3 cut(s) 457, 874, 1271
SfaNI GCATC 1 cut(s) 305
SfcI CTRYAG 1 cut(s) 1043
SfuI TTCGAA 1 cut(s) 23
SinI GGWCC 2 cut(s) 468, 1151
SpeI ACTAGT 1 cut(s) 625
SsiI CCGC 2 cut(s) 617, 633
SspMI CTAG 4 cut(s) 626, 731, 765, 1064
SstI GAGCTC 2 cut(s) 457, 1271
StyD4I CCNGG 1 cut(s) 975
StyI CCWWGG 1 cut(s) 345
TaaI ACNGT 1 cut(s) 103
TaiI ACGT 2 cut(s) 1016, 1135
TaqI TCGA 4 cut(s) 23, 1119, 1125, 1322
TatI WGTACW 2 cut(s) 442, 1006
TauI GCSGC 1 cut(s) 635
TfiI GAWTC 1 cut(s) 983
Tru1I TTAA 5 cut(s) 257, 782, 881, 891, 1077
Tru9I TTAA 5 cut(s) 257, 782, 881, 891, 1077
TseI GCWGC 3 cut(s) 761, 1042, 1338
TspDTI ATGAA 6 cut(s) 38, 351, 356, 549, 702, 834
VpaK11BI GGWCC 2 cut(s) 468, 1151
XapI RAATTY 4 cut(s) 724, 936, 1111, 1144
XceI RCATGY 1 cut(s) 1228
XmiI GTMKAC 1 cut(s) 1278
XmnI GAANNNNTTC 1 cut(s) 1115
XspI CTAG 4 cut(s) 626, 731, 765, 1064
ZrmI AGTACT 1 cut(s) 1008
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.