Rorug07G0206900

(S)-coclaurine N-methyltransferase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0002136
Physical Location & Seq
Forward (+)
18233899 .. 18239728
5830 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0206900.1

Sequence Viewer

Length: 1431 bp
ATGACGACCCTTTCTTCAATTTCTCGGTTCCTCTCTTCCTCCATTCGCCTAAAAAATCACAAGCTCTCAATCTCTCACTTCTCTACTGCCTCGTCGGCAGAGACGGTCTACAATCACCTCCAGAAAAATGGTGGCAACATTGAGAAAACCCTTGCCTCAATGAGGCTCAATTTGGACTCCAAATGTGTAAGTCAGGTTTTACATAGATGCTATCCCACCCAATCCCAAATGGGTCTCAGATTTTTCATATGGGCTGGGGTTCACTCCAGTTATAGGCATAGCTATTTTATGTTCAGCAAAGCTTGTGAATTGTATAGGATTAGGCAAAACCCAAGTGTGATTTTTGATGTTTTGGAGGCTTATAGTGTTGAAGGTTGCTCTGTTAATATGAAAATGTTTAAAGTTGTTTTCAATATGTGTAAAGAAGCTAAGCTTGCTGATGAGGCTTTGAGGGTTCTGAGGAAAATGCCGGAGTTTGGGCTGCGAGGGGATAATGTAGTGTATAATGTTGTTATAAGGTTGTTTTGTGATAAGGGTGATTTGGATATGGCTGAGAGTTTGGTGAAGGAGATGAGTATGGTGGAGCTTTATCCGGATTTGATCACATACATGGTGATGATTAAAGGGTTGTGTAATGTGGGTCGGTTGGATGATGCGTGTGGGTTGTTTAAGTTTATGAAGGAAAATGGTTGTTTGCCTAATGTTGTGGTGTACTCGGCCCTTCTCGATGGATTCTGTAGGTTTGGGGATATGGAGAGAGCCTTGAAGTTGTTAGAGGAGATGGAGAAGGAAGGTGGGGATTGTAGTCCTAATGTAGTAACATATACATCTGTGATTCAATATCTTTGTGACAAGGGTCAGTCAGTGGAGGCATTGCTTGTTTTGGACCGAATGGAAGCTCGTGGTTGTTTACCAACTCGCGTCACGGTTAGTTCTTTGATCACGGGTTTTGTTAAGGAGGATCAAGTTGAGCAGGCTTATAAGTTGGTTGATAGAGTTGTTGAAAGTGGAAGTGTTACAAAAACTGATTGCTATAGTTCTCTTGTTGTGGCTTTGGAAAGAGTCAGAAAACCCGAGGAGGCAGAGAAAGTCTTTAGGACGATGCTTGATAGTGGGGTCAAACCAAACAGTTTGGTGTCTACCATTATGTTAAAAAAACGTTGTTTGGAGGGACGTATGGTGGATGCATATTGCTTGTTTGATGAACTTGAGAAGATGGAATGCTTATCTTCTATTGATTCTGATACTTATTCTATTCTTTTAGTGGGGCTTTGTCAGCAAAGACACTTAATGGAGGCTGCAAAGCTTGCAAGGGTAATGCTGAGTAAAGGAATTAAACTAAAAGCTCCATATGTTGATATTATAGCTGAGGTCCTGATGAAATCTGGAGATGAGGAGTTACTGGAACAATTGACAAGAAATGGAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CMAS

Protein Analysis

476

Amino Acids

53.73

Weight (kDa)

7.41

Isoelectric Point (pI)

32.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 117 - 220 2e-10 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 164 - 212 3e-12 PPR repeat family
PPR_3 PF13812 186 - 243 1.3e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 196 - 227 1.7e-08 PPR repeat
PPR_2 PF13041 198 - 247 7.2e-16 PPR repeat family
PPR PF01535 201 - 231 3.3e-07 PPR repeat
PPR_long PF17177 218 - 336 4.7e-09 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 229 - 262 7.7e-13 PPR repeat
PPR_2 PF13041 233 - 265 1e-08 PPR repeat family
PPR_3 PF13812 233 - 281 2.7e-08 Pentatricopeptide repeat domain
PPR PF01535 236 - 265 2.5e-08 PPR repeat
PPR_3 PF13812 256 - 319 1.7e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 267 - 299 1.3e-07 PPR repeat
PPR_2 PF13041 270 - 319 2e-13 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 515, 981
AccI GTMKAC 2 cut(s) 108, 1139
AccII CGCG 1 cut(s) 921
AccIII TCCGGA 1 cut(s) 592
AclI AACGTT 1 cut(s) 1159
AclWI GGATC 1 cut(s) 969
AfaI GTAC 1 cut(s) 713
AfiI CCNNNNNNNGG 3 cut(s) 162, 273, 476
AgsI TTSAA 6 cut(s) 18, 371, 412, 766, 839, 1004
Alw26I GTCTC 2 cut(s) 95, 239
AlwI GGATC 1 cut(s) 969
Ama87I CYCGRG 1 cut(s) 1073
Aor13HI TCCGGA 1 cut(s) 592
AoxI GGCC 1 cut(s) 717
ApeKI GCWGC 2 cut(s) 481, 1298
AspS9I GGNCC 3 cut(s) 718, 886, 1372
AsuHPI GGTGA 4 cut(s) 107, 548, 574, 625
AvaI CYCGRG 1 cut(s) 1073
AvaII GGWCC 2 cut(s) 886, 1372
BauI CACGAG 1 cut(s) 900
BbvCI CCTCAGC 1 cut(s) 1368
BbvI GCAGC 2 cut(s) 468, 1285
BccI CCATC 3 cut(s) 722, 775, 1210
BclI TGATCA 2 cut(s) 600, 939
BcoDI GTCTC 2 cut(s) 95, 239
BfmI CTRYAG 2 cut(s) 736, 1033
BglI GCCNNNNNGGC 1 cut(s) 95
BisI GCNGC 2 cut(s) 482, 1299
BlpI GCTNAGC 1 cut(s) 429
BlsI GCNGC 2 cut(s) 483, 1300
Bme18I GGWCC 2 cut(s) 886, 1372
BmeT110I CYCGRG 1 cut(s) 1073
BmgT120I GGNCC 3 cut(s) 718, 886, 1372
BmiI GGNNCC 1 cut(s) 29
BmsI GCATC 4 cut(s) 197, 643, 1092, 1174
BoxI GACNNNNGTC 1 cut(s) 855
BpmI CTGGAG 3 cut(s) 104, 250, 1407
Bpu10I CCTNAGC 1 cut(s) 1368
Bpu1102I GCTNAGC 1 cut(s) 429
BpuEI CTTGAG 1 cut(s) 1229
BsaI GGTCTC 1 cut(s) 239
BsaJI CCNNGG 1 cut(s) 1074
BsaWI WCCGGW 1 cut(s) 592
Bsc4I CCNNNNNNNGG 3 cut(s) 162, 273, 476
Bse1I ACTGG 2 cut(s) 267, 1407
Bse3DI GCAATG 1 cut(s) 872
BseAI TCCGGA 1 cut(s) 592
BseDI CCNNGG 1 cut(s) 1074
BseGI GGATG 2 cut(s) 655, 1189
BseLI CCNNNNNNNGG 3 cut(s) 162, 273, 476
BseMI GCAATG 1 cut(s) 872
BseMII CTCAG 5 cut(s) 250, 449, 543, 1313, 1359
BseNI ACTGG 2 cut(s) 267, 1407
BseRI GAGGAG 3 cut(s) 791, 1091, 1409
BseXI GCAGC 2 cut(s) 468, 1285
BseYI CCCAGC 1 cut(s) 254
Bsh1236I CGCG 1 cut(s) 921
BshFI GGCC 1 cut(s) 719
BsiHKCI CYCGRG 1 cut(s) 1073
BsiSI CCGG 2 cut(s) 470, 593
BslFI GGGAC 1 cut(s) 1185
BslI CCNNNNNNNGG 3 cut(s) 162, 273, 476
BsmAI GTCTC 2 cut(s) 95, 239
BsmBI CGTCTC 1 cut(s) 95
BsmFI GGGAC 1 cut(s) 1185
BsmI GAATGC 1 cut(s) 1226
BsnI GGCC 1 cut(s) 719
Bso31I GGTCTC 1 cut(s) 239
BsoBI CYCGRG 1 cut(s) 1073
Bsp13I TCCGGA 1 cut(s) 592
Bsp143I GATC 3 cut(s) 600, 939, 961
Bsp1720I GCTNAGC 1 cut(s) 429
BspANI GGCC 1 cut(s) 719
BspCNI CTCAG 5 cut(s) 249, 450, 544, 1314, 1360
BspEI TCCGGA 1 cut(s) 592
BspFNI CGCG 1 cut(s) 921
BspLI GGNNCC 1 cut(s) 29
BspPI GGATC 1 cut(s) 969
BspTNI GGTCTC 1 cut(s) 239
BsrDI GCAATG 1 cut(s) 872
BsrI ACTGG 2 cut(s) 267, 1407
BssECI CCNNGG 1 cut(s) 1074
BssMI GATC 3 cut(s) 600, 939, 961
BssSI CACGAG 1 cut(s) 900
Bst2BI CACGAG 1 cut(s) 900
Bst4CI ACNGT 3 cut(s) 106, 928, 1130
Bst6I CTCTTC 1 cut(s) 40
BstAPI GCANNNNNTGC 1 cut(s) 1307
BstC8I GCNNGC 3 cut(s) 435, 975, 1308
BstDEI CTNAG 6 cut(s) 236, 429, 458, 552, 1322, 1368
BstENI CCTNNNNNAGG 1 cut(s) 160
BstF5I GGATG 2 cut(s) 655, 1189
BstFNI CGCG 1 cut(s) 921
BstKTI GATC 3 cut(s) 603, 942, 964
BstMAI GTCTC 2 cut(s) 95, 239
BstMBI GATC 3 cut(s) 600, 939, 961
BstMWI GCNNNNNNNGC 5 cut(s) 95, 434, 443, 1276, 1307
BstPAI GACNNNNGTC 1 cut(s) 855
BstSFI CTRYAG 2 cut(s) 736, 1033
BstUI CGCG 1 cut(s) 921
BstV1I GCAGC 2 cut(s) 468, 1285
BstXI CCANNNNNNTGG 1 cut(s) 128
BsuRI GGCC 1 cut(s) 719
BtsCI GGATG 2 cut(s) 655, 1189
BtsIMutI CAGTG 1 cut(s) 870
Cac8I GCNNGC 3 cut(s) 435, 975, 1308
Cfr13I GGNCC 3 cut(s) 718, 886, 1372
CseI GACGC 1 cut(s) 910
Csp6I GTAC 1 cut(s) 712
CviAII CATG 1 cut(s) 610
CviQI GTAC 1 cut(s) 712
DdeI CTNAG 6 cut(s) 236, 429, 458, 552, 1322, 1368
DpnI GATC 3 cut(s) 602, 941, 963
DpnII GATC 3 cut(s) 600, 939, 961
DraI TTTAAA 1 cut(s) 400
Eam1104I CTCTTC 1 cut(s) 40
EarI CTCTTC 1 cut(s) 40
Eco31I GGTCTC 1 cut(s) 239
Eco47I GGWCC 2 cut(s) 886, 1372
Eco88I CYCGRG 1 cut(s) 1073
EcoNI CCTNNNNNAGG 1 cut(s) 160
EcoO109I RGGNCCY 1 cut(s) 1372
EcoT22I ATGCAT 1 cut(s) 1189
Esp3I CGTCTC 1 cut(s) 95
FaeI CATG 1 cut(s) 613
FalI AAGNNNNNCTT 2 cut(s) 417, 449
FaqI GGGAC 1 cut(s) 1185
FatI CATG 1 cut(s) 609
FauNDI CATATG 2 cut(s) 248, 1351
FbaI TGATCA 2 cut(s) 600, 939
FblI GTMKAC 2 cut(s) 108, 1139
Fnu4HI GCNGC 2 cut(s) 482, 1299
FokI GGATG 2 cut(s) 662, 1196
Fsp4HI GCNGC 2 cut(s) 482, 1299
GluI GCNGC 2 cut(s) 482, 1299
GsaI CCCAGC 1 cut(s) 258
GsuI CTGGAG 3 cut(s) 104, 250, 1407
HaeIII GGCC 1 cut(s) 719
HapII CCGG 2 cut(s) 470, 593
HgaI GACGC 1 cut(s) 910
Hin1II CATG 1 cut(s) 613
HindIII AAGCTT 3 cut(s) 300, 431, 1304
HinfI GANTC 5 cut(s) 176, 732, 835, 1062, 1238
HpaII CCGG 2 cut(s) 470, 593
HphI GGTGA 4 cut(s) 107, 548, 574, 625
Hpy166II GTNNAC 5 cut(s) 109, 262, 712, 911, 1140
Hpy188I TCNGA 4 cut(s) 239, 459, 1067, 1243
Hpy188III TCNNGA 5 cut(s) 121, 593, 725, 1375, 1386
Hpy8I GTNNAC 5 cut(s) 109, 262, 712, 911, 1140
Hpy99I CGWCG 1 cut(s) 97
HpyAV CCTTC 7 cut(s) 365, 559, 673, 731, 781, 785, 1419
HpyCH4III ACNGT 3 cut(s) 106, 928, 1130
HpyCH4IV ACGT 2 cut(s) 1159, 1174
HpyCH4V TGCA 3 cut(s) 1187, 1301, 1310
HpyF10VI GCNNNNNNNGC 5 cut(s) 95, 434, 443, 1276, 1307
HpyF3I CTNAG 6 cut(s) 236, 429, 458, 552, 1322, 1368
HpySE526I ACGT 2 cut(s) 1159, 1174
Hsp92II CATG 1 cut(s) 613
Kpn2I TCCGGA 1 cut(s) 592
Ksp22I TGATCA 2 cut(s) 600, 939
Kzo9I GATC 3 cut(s) 600, 939, 961
LmnI GCTCC 2 cut(s) 583, 1351
Lsp1109I GCAGC 2 cut(s) 468, 1285
LweI GCATC 4 cut(s) 197, 643, 1092, 1174
MaeII ACGT 2 cut(s) 1159, 1174
MaeIII GTNAC 5 cut(s) 817, 848, 922, 1015, 1398
MalI GATC 3 cut(s) 602, 941, 963
MboI GATC 3 cut(s) 600, 939, 961
MboII GAAGA 4 cut(s) 6, 27, 1221, 1225
MfeI CAATTG 1 cut(s) 1409
MluCI AATT 5 cut(s) 18, 169, 308, 1332, 1409
MlyI GAGTC 2 cut(s) 170, 1071
MmeI TCCRAC 1 cut(s) 627
Mph1103I ATGCAT 1 cut(s) 1189
MroI TCCGGA 1 cut(s) 592
MseI TTAA 8 cut(s) 384, 399, 621, 669, 954, 1151, 1289, 1335
MslI CAYNNNNRTG 2 cut(s) 608, 614
MspI CCGG 2 cut(s) 470, 593
MunI CAATTG 1 cut(s) 1409
Mva1269I GAATGC 1 cut(s) 1226
MvnI CGCG 1 cut(s) 921
MwoI GCNNNNNNNGC 5 cut(s) 95, 434, 443, 1276, 1307
NdeI CATATG 2 cut(s) 248, 1351
NdeII GATC 3 cut(s) 600, 939, 961
NlaIII CATG 1 cut(s) 613
NlaIV GGNNCC 1 cut(s) 29
NmeAIII GCCGAG 1 cut(s) 695
NmuCI GTSAC 2 cut(s) 848, 922
NsiI ATGCAT 1 cut(s) 1189
PctI GAATGC 1 cut(s) 1226
PfeI GAWTC 3 cut(s) 732, 835, 1238
PkrI GCNGC 2 cut(s) 483, 1300
PleI GAGTC 2 cut(s) 170, 1070
PpsI GAGTC 2 cut(s) 170, 1070
PpuMI RGGWCCY 1 cut(s) 1372
PshAI GACNNNNGTC 1 cut(s) 855
PsiI TTATAA 2 cut(s) 515, 981
Psp1406I AACGTT 1 cut(s) 1159
Psp5II RGGWCCY 1 cut(s) 1372
PspFI CCCAGC 1 cut(s) 254
PspN4I GGNNCC 1 cut(s) 29
PspPI GGNCC 3 cut(s) 718, 886, 1372
PspPPI RGGWCCY 1 cut(s) 1372
RsaI GTAC 1 cut(s) 713
RsaNI GTAC 1 cut(s) 712
RseI CAYNNNNRTG 2 cut(s) 608, 614
SaqAI TTAA 8 cut(s) 384, 399, 621, 669, 954, 1151, 1289, 1335
SatI GCNGC 2 cut(s) 482, 1299
Sau3AI GATC 3 cut(s) 600, 939, 961
Sau96I GGNCC 3 cut(s) 718, 886, 1372
SchI GAGTC 2 cut(s) 170, 1071
SfaNI GCATC 4 cut(s) 197, 643, 1092, 1174
SfcI CTRYAG 2 cut(s) 736, 1033
SinI GGWCC 2 cut(s) 886, 1372
SmiMI CAYNNNNRTG 2 cut(s) 608, 614
SmlI CTYRAG 1 cut(s) 1208
SmoI CTYRAG 1 cut(s) 1208
Sse9I AATT 5 cut(s) 18, 169, 308, 1332, 1409
TaaI ACNGT 3 cut(s) 106, 928, 1130
TaiI ACGT 2 cut(s) 1162, 1177
TaqI TCGA 1 cut(s) 726
TaqII GACCGA 1 cut(s) 903
TasI AATT 5 cut(s) 18, 169, 308, 1332, 1409
TatI WGTACW 1 cut(s) 711
TfiI GAWTC 3 cut(s) 732, 835, 1238
Tru1I TTAA 8 cut(s) 384, 399, 621, 669, 954, 1151, 1289, 1335
Tru9I TTAA 8 cut(s) 384, 399, 621, 669, 954, 1151, 1289, 1335
TscAI CASTG 1 cut(s) 870
TseFI GTSAC 2 cut(s) 848, 922
TseI GCWGC 2 cut(s) 481, 1298
Tsp45I GTSAC 2 cut(s) 848, 922
TspDTI ATGAA 5 cut(s) 235, 404, 692, 1218, 1394
TspRI CASTG 1 cut(s) 870
VpaK11BI GGWCC 2 cut(s) 886, 1372
XagI CCTNNNNNAGG 1 cut(s) 160
XcmI CCANNNNNNNNNTGG 1 cut(s) 128
XmiI GTMKAC 2 cut(s) 108, 1139
Zsp2I ATGCAT 1 cut(s) 1189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.