Rorug06G0091700

Belongs to the TUB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0003737
Physical Location & Seq
Forward (+)
12243295 .. 12244239
945 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0091700.1

Sequence Viewer

Length: 945 bp
ATGAGCGACGAACTAGCCCATGATTTCTCTCCCATGATCAAAGTATACAAAGACGGTCGAATCGACCGACCCTGGGGAACAACCACAGTCCCTGCATCCATCGATCCCCAAACCGGTGTTCAATCCAAAGACGTCGTCATCTCAACCGCACCACCCATCTCTGTAAGGCTTTACATTCCCAAATCCAACACAACCGAGTCAACCCGGAAGCTCCCTCTTCTGGTTTACTTTCACGGCGGTGCCTTCTGCATTGGAAGTGCTTTCTCTCGCATATGTCACAACCACTGCAACTCTTTAGTCTCTGAAGCCAATGTTGTAGCTGTTTCTGTTGACTATAGGTTGGTGCCGGAGCACCCTCTTCCGGCTGCCTATGATGATTCATGGGCCGCTCTCAAATGGGTGGAGTCCCATTTCGCCGGAACCGGCCCGGAAGAGTGGCTGAACCGGCATGCAGATTTGAACCGGGTTTTCTTTTCAGGTGATAGCGCTGGGGCAAACATAGCTCACCACATGGGTTTGAGAGTTGGGTCTGAGAAGTTGGTGTATTTTAAGCTGAATGGGATTGTTCTGGTGCATCCATACTTTTGGGGTGAAGAACCAGTAGGCGGAGAGGTAGCTTTGGGCGAGAGTCATAGAGAGCATTTGGATGCTTTGTGGAGGTTTGCTTACCCTTTGACTAGTGGATCTGATGACCCGCTTTTTAATCCGGCTAAGGATCCGAAGTTGGGAGAGTTGGGTTGTGAGAAAGTGTTGGTCTGTGTTGCGGAGAAGGATGCGTTGAAAGATAGGGGGTGGTATTACAGTGAGGTGTTGAAGAAGAGTGGATGGAATGGAGTAGTAGAGGTTTGGGAAACAAAGGAGGAGCAGCATGTGTTCCATTTGATCAATCCTAGTTGTGACAATGCTGTGACCATGCTCAAGAAGATTGTTTCTTTCATGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005543 GO:0008289 GO:0035091 GO:0043167 GO:0043168
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box Tub

Protein Analysis

314

Amino Acids

34.93

Weight (kDa)

5.86

Isoelectric Point (pI)

27.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 57 - 177 1.2e-09 Carboxylesterase family
BD-FAE PF20434 66 - 178 1.9e-12 BD-FAE
Abhydrolase_3 PF07859 74 - 293 1.9e-54 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 135
AccB1I GGYRCC 2 cut(s) 239, 343
AccBSI CCGCTC 1 cut(s) 389
AccI GTMKAC 1 cut(s) 45
AciI CCGC 6 cut(s) 147, 237, 387, 606, 695, 764
AclWI GGATC 4 cut(s) 98, 691, 710, 723
AcuI CTGAAG 1 cut(s) 324
AcyI GRCGYC 1 cut(s) 132
AfeI AGCGCT 1 cut(s) 487
AfiI CCNNNNNNNGG 6 cut(s) 73, 113, 220, 361, 605, 725
AgeI ACCGGT 1 cut(s) 113
AgsI TTSAA 4 cut(s) 122, 460, 781, 814
AhlI ACTAGT 1 cut(s) 677
AjnI CCWGG 1 cut(s) 71
AjuI GAANNNNNNNTTGG 2 cut(s) 102, 134
AleI CACNNNNGTG 1 cut(s) 237
AluBI AGCT 5 cut(s) 211, 320, 503, 553, 617
AluI AGCT 5 cut(s) 211, 320, 503, 553, 617
Alw21I GWGCWC 1 cut(s) 354
Alw26I GTCTC 1 cut(s) 304
AlwI GGATC 4 cut(s) 98, 691, 710, 723
AlwNI CAGNNNCTG 1 cut(s) 92
Aor51HI AGCGCT 1 cut(s) 487
AoxI GGCC 2 cut(s) 384, 424
ApeKI GCWGC 2 cut(s) 365, 865
ArsI GACNNNNNNTTYG 2 cut(s) 120, 152
AsiGI ACCGGT 1 cut(s) 113
AspLEI GCGC 1 cut(s) 488
AspS9I GGNCC 2 cut(s) 384, 425
AsuC2I CCSGG 3 cut(s) 205, 428, 464
AsuHPI GGTGA 3 cut(s) 491, 497, 602
BamHI GGATCC 1 cut(s) 715
BanI GGYRCC 2 cut(s) 239, 343
Bbv12I GWGCWC 1 cut(s) 354
BbvI GCAGC 2 cut(s) 352, 877
BccI CCATC 3 cut(s) 107, 164, 819
BceAI ACGGC 1 cut(s) 250
BciT130I CCWGG 1 cut(s) 73
BclI TGATCA 2 cut(s) 36, 882
BcnI CCSGG 3 cut(s) 205, 428, 464
BcoDI GTCTC 1 cut(s) 304
BcuI ACTAGT 1 cut(s) 677
BfaI CTAG 3 cut(s) 14, 678, 891
BfmI CTRYAG 1 cut(s) 334
BfoI RGCGCY 1 cut(s) 489
BisI GCNGC 3 cut(s) 366, 387, 866
BlsI GCNGC 3 cut(s) 367, 388, 867
Bme1390I CCNGG 4 cut(s) 73, 205, 428, 464
BmgT120I GGNCC 2 cut(s) 384, 425
BmiI GGNNCC 4 cut(s) 241, 345, 421, 717
BmrFI CCNGG 4 cut(s) 73, 205, 428, 464
BmsI GCATC 4 cut(s) 104, 583, 637, 763
Bpu10I CCTNAGC 1 cut(s) 711
BpuEI CTTGAG 1 cut(s) 902
BpuMI CCSGG 3 cut(s) 205, 428, 464
Bsa29I ATCGAT 1 cut(s) 102
BsaHI GRCGYC 1 cut(s) 132
BsaJI CCNNGG 2 cut(s) 71, 72
BsaWI WCCGGW 1 cut(s) 113
Bsc4I CCNNNNNNNGG 6 cut(s) 73, 113, 220, 361, 605, 725
Bse118I RCCGGY 3 cut(s) 113, 422, 444
Bse1I ACTGG 1 cut(s) 599
BseBI CCWGG 1 cut(s) 73
BseCI ATCGAT 1 cut(s) 102
BseDI CCNNGG 2 cut(s) 71, 72
BseGI GGATG 5 cut(s) 95, 574, 652, 778, 830
BseLI CCNNNNNNNGG 6 cut(s) 73, 113, 220, 361, 605, 725
BseMII CTCAG 1 cut(s) 522
BseNI ACTGG 1 cut(s) 599
BseRI GAGGAG 1 cut(s) 875
BseXI GCAGC 2 cut(s) 352, 877
BseYI CCCAGC 1 cut(s) 488
Bsh1285I CGRYCG 2 cut(s) 58, 67
BshFI GGCC 2 cut(s) 386, 426
BshNI GGYRCC 2 cut(s) 239, 343
BshTI ACCGGT 1 cut(s) 113
BshVI ATCGAT 1 cut(s) 102
BsiEI CGRYCG 2 cut(s) 58, 67
BsiHKAI GWGCWC 1 cut(s) 354
BslFI GGGAC 2 cut(s) 74, 391
BslI CCNNNNNNNGG 6 cut(s) 73, 113, 220, 361, 605, 725
BsmAI GTCTC 1 cut(s) 304
BsmFI GGGAC 2 cut(s) 74, 391
BsnI GGCC 2 cut(s) 386, 426
Bsp1286I GDGCHC 1 cut(s) 354
Bsp143I GATC 5 cut(s) 36, 103, 683, 715, 882
BspACI CCGC 6 cut(s) 147, 237, 387, 606, 695, 764
BspANI GGCC 2 cut(s) 386, 426
BspCNI CTCAG 1 cut(s) 523
BspDI ATCGAT 1 cut(s) 102
BspHI TCATGA 1 cut(s) 936
BspLI GGNNCC 4 cut(s) 241, 345, 421, 717
BspPI GGATC 4 cut(s) 98, 691, 710, 723
BspT107I GGYRCC 2 cut(s) 239, 343
BsrBI CCGCTC 1 cut(s) 389
BsrFI RCCGGY 3 cut(s) 113, 422, 444
BsrI ACTGG 1 cut(s) 599
BssAI RCCGGY 3 cut(s) 113, 422, 444
BssECI CCNNGG 2 cut(s) 71, 72
BssMI GATC 5 cut(s) 36, 103, 683, 715, 882
BssNAI GTATAC 1 cut(s) 46
BssNI GRCGYC 1 cut(s) 132
Bst1107I GTATAC 1 cut(s) 46
Bst2UI CCWGG 1 cut(s) 73
Bst4CI ACNGT 3 cut(s) 56, 88, 803
Bst6I CTCTTC 4 cut(s) 222, 363, 426, 812
BstACI GRCGYC 1 cut(s) 132
BstC8I GCNNGC 1 cut(s) 450
BstDEI CTNAG 2 cut(s) 531, 711
BstF5I GGATG 5 cut(s) 95, 574, 652, 778, 830
BstH2I RGCGCY 1 cut(s) 489
BstHHI GCGC 1 cut(s) 488
BstKTI GATC 5 cut(s) 39, 106, 686, 718, 885
BstMAI GTCTC 1 cut(s) 304
BstMBI GATC 5 cut(s) 36, 103, 683, 715, 882
BstMCI CGRYCG 2 cut(s) 58, 67
BstMWI GCNNNNNNNGC 2 cut(s) 445, 500
BstNI CCWGG 1 cut(s) 73
BstNSI RCATGY 2 cut(s) 452, 872
BstSCI CCNGG 4 cut(s) 71, 203, 426, 462
BstSFI CTRYAG 1 cut(s) 334
BstV1I GCAGC 2 cut(s) 352, 877
BstX2I RGATCY 2 cut(s) 683, 715
BstXI CCANNNNNNTGG 1 cut(s) 585
BstYI RGATCY 2 cut(s) 683, 715
BstZ17I GTATAC 1 cut(s) 46
Bsu15I ATCGAT 1 cut(s) 102
BsuRI GGCC 2 cut(s) 386, 426
BsuTUI ATCGAT 1 cut(s) 102
BtsCI GGATG 5 cut(s) 95, 574, 652, 778, 830
BtsI GCAGTG 1 cut(s) 283
BtsIMutI CAGTG 2 cut(s) 283, 808
Cac8I GCNNGC 1 cut(s) 450
CaiI CAGNNNCTG 1 cut(s) 92
CciI TCATGA 1 cut(s) 936
CfoI GCGC 1 cut(s) 488
Cfr10I RCCGGY 3 cut(s) 113, 422, 444
Cfr13I GGNCC 2 cut(s) 384, 425
ClaI ATCGAT 1 cut(s) 102
CspAI ACCGGT 1 cut(s) 113
CviAII CATG 8 cut(s) 20, 34, 381, 449, 511, 869, 913, 937
DdeI CTNAG 2 cut(s) 531, 711
DpnI GATC 5 cut(s) 38, 105, 685, 717, 884
DpnII GATC 5 cut(s) 36, 103, 683, 715, 882
Eam1104I CTCTTC 4 cut(s) 222, 363, 426, 812
EarI CTCTTC 4 cut(s) 222, 363, 426, 812
EciI GGCGGA 1 cut(s) 621
Eco47III AGCGCT 1 cut(s) 487
Eco57I CTGAAG 1 cut(s) 324
EcoRII CCWGG 1 cut(s) 71
FaeI CATG 8 cut(s) 23, 37, 384, 452, 514, 872, 916, 940
FaqI GGGAC 2 cut(s) 74, 391
FatI CATG 8 cut(s) 19, 33, 380, 448, 510, 868, 912, 936
FauI CCCGC 1 cut(s) 702
FauNDI CATATG 1 cut(s) 272
FbaI TGATCA 2 cut(s) 36, 882
FblI GTMKAC 1 cut(s) 45
Fnu4HI GCNGC 3 cut(s) 366, 387, 866
FokI GGATG 5 cut(s) 82, 561, 659, 785, 837
Fsp4HI GCNGC 3 cut(s) 366, 387, 866
FspBI CTAG 3 cut(s) 14, 678, 891
GlaI GCGC 1 cut(s) 487
GluI GCNGC 3 cut(s) 366, 387, 866
GsaI CCCAGC 1 cut(s) 492
HaeII RGCGCY 1 cut(s) 489
HaeIII GGCC 2 cut(s) 386, 426
HhaI GCGC 1 cut(s) 488
Hin1I GRCGYC 1 cut(s) 132
Hin1II CATG 8 cut(s) 23, 37, 384, 452, 514, 872, 916, 940
Hin6I GCGC 1 cut(s) 486
HinP1I GCGC 1 cut(s) 486
HincII GTYRAC 2 cut(s) 201, 331
HindII GTYRAC 2 cut(s) 201, 331
HinfI GANTC 5 cut(s) 60, 197, 377, 404, 628
HphI GGTGA 3 cut(s) 491, 497, 602
Hpy166II GTNNAC 4 cut(s) 46, 201, 226, 331
Hpy188I TCNGA 4 cut(s) 304, 532, 688, 720
Hpy188III TCNNGA 2 cut(s) 919, 937
Hpy8I GTNNAC 4 cut(s) 46, 201, 226, 331
Hpy99I CGWCG 2 cut(s) 11, 137
HpyAV CCTTC 2 cut(s) 253, 763
HpyCH4III ACNGT 3 cut(s) 56, 88, 803
HpyCH4IV ACGT 1 cut(s) 132
HpyCH4V TGCA 5 cut(s) 95, 249, 288, 452, 574
HpyF10VI GCNNNNNNNGC 2 cut(s) 445, 500
HpyF3I CTNAG 2 cut(s) 531, 711
HpySE526I ACGT 1 cut(s) 132
Hsp92I GRCGYC 1 cut(s) 132
Hsp92II CATG 8 cut(s) 23, 37, 384, 452, 514, 872, 916, 940
HspAI GCGC 1 cut(s) 486
Ksp22I TGATCA 2 cut(s) 36, 882
Kzo9I GATC 5 cut(s) 36, 103, 683, 715, 882
LmnI GCTCC 3 cut(s) 216, 349, 862
Lsp1109I GCAGC 2 cut(s) 352, 877
LweI GCATC 4 cut(s) 104, 583, 637, 763
MaeI CTAG 3 cut(s) 14, 678, 891
MaeII ACGT 1 cut(s) 132
MaeIII GTNAC 3 cut(s) 275, 896, 907
MalI GATC 5 cut(s) 38, 105, 685, 717, 884
MbiI CCGCTC 1 cut(s) 389
MboI GATC 5 cut(s) 36, 103, 683, 715, 882
MboII GAAGA 7 cut(s) 209, 350, 443, 605, 826, 829, 934
MflI RGATCY 2 cut(s) 683, 715
MhlI GDGCHC 1 cut(s) 354
MluCI AATT 1 cut(s) 940
MlyI GAGTC 3 cut(s) 206, 413, 637
MmeI TCCRAC 1 cut(s) 210
MnlI CCTC 7 cut(s) 225, 366, 604, 651, 799, 835, 853
MseI TTAA 2 cut(s) 549, 702
MslI CAYNNNNRTG 2 cut(s) 237, 645
MspR9I CCNGG 4 cut(s) 73, 205, 428, 464
MvaI CCWGG 1 cut(s) 73
MwoI GCNNNNNNNGC 2 cut(s) 445, 500
NciI CCSGG 3 cut(s) 205, 428, 464
NdeI CATATG 1 cut(s) 272
NdeII GATC 5 cut(s) 36, 103, 683, 715, 882
NlaIII CATG 8 cut(s) 23, 37, 384, 452, 514, 872, 916, 940
NlaIV GGNNCC 4 cut(s) 241, 345, 421, 717
NmuCI GTSAC 3 cut(s) 275, 896, 907
NspI RCATGY 2 cut(s) 452, 872
OliI CACNNNNGTG 1 cut(s) 237
PaeI GCATGC 1 cut(s) 452
PagI TCATGA 1 cut(s) 936
PasI CCCWGGG 1 cut(s) 72
PcsI WCGNNNNNNNCGW 2 cut(s) 60, 64
PfeI GAWTC 2 cut(s) 60, 377
PflFI GACNNNGTC 1 cut(s) 134
PinAI ACCGGT 1 cut(s) 113
PkrI GCNGC 3 cut(s) 367, 388, 867
PleI GAGTC 3 cut(s) 205, 412, 636
PpsI GAGTC 3 cut(s) 205, 412, 636
Psp6I CCWGG 1 cut(s) 71
PspFI CCCAGC 1 cut(s) 488
PspGI CCWGG 1 cut(s) 71
PspN4I GGNNCC 4 cut(s) 241, 345, 421, 717
PspPI GGNCC 2 cut(s) 384, 425
PstNI CAGNNNCTG 1 cut(s) 92
PsuI RGATCY 2 cut(s) 683, 715
PsyI GACNNNGTC 1 cut(s) 134
RseI CAYNNNNRTG 2 cut(s) 237, 645
SaqAI TTAA 2 cut(s) 549, 702
SatI GCNGC 3 cut(s) 366, 387, 866
Sau3AI GATC 5 cut(s) 36, 103, 683, 715, 882
Sau96I GGNCC 2 cut(s) 384, 425
SchI GAGTC 3 cut(s) 206, 413, 637
ScrFI CCNGG 4 cut(s) 73, 205, 428, 464
SduI GDGCHC 1 cut(s) 354
SfaNI GCATC 4 cut(s) 104, 583, 637, 763
SfcI CTRYAG 1 cut(s) 334
SmiMI CAYNNNNRTG 2 cut(s) 237, 645
SmlI CTYRAG 1 cut(s) 917
SmoI CTYRAG 1 cut(s) 917
SpeI ACTAGT 1 cut(s) 677
SphI GCATGC 1 cut(s) 452
Sse9I AATT 1 cut(s) 940
SsiI CCGC 6 cut(s) 147, 237, 387, 606, 695, 764
SspMI CTAG 3 cut(s) 14, 678, 891
StyD4I CCNGG 4 cut(s) 71, 203, 426, 462
TaaI ACNGT 3 cut(s) 56, 88, 803
TaiI ACGT 1 cut(s) 135
TaqI TCGA 3 cut(s) 58, 63, 102
TaqII GACCGA 1 cut(s) 81
TasI AATT 1 cut(s) 940
TauI GCSGC 1 cut(s) 389
TfiI GAWTC 2 cut(s) 60, 377
Tru1I TTAA 2 cut(s) 549, 702
Tru9I TTAA 2 cut(s) 549, 702
TscAI CASTG 2 cut(s) 290, 808
TseFI GTSAC 3 cut(s) 275, 896, 907
TseI GCWGC 2 cut(s) 365, 865
Tsp45I GTSAC 3 cut(s) 275, 896, 907
TspDTI ATGAA 2 cut(s) 369, 925
TspRI CASTG 2 cut(s) 290, 808
Tth111I GACNNNGTC 1 cut(s) 134
XceI RCATGY 2 cut(s) 452, 872
XmiI GTMKAC 1 cut(s) 45
XspI CTAG 3 cut(s) 14, 678, 891
ZraI GACGTC 1 cut(s) 133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.