Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000006

Orthogroup ID

OG0001036

Physical Location & Seq

Genomic Coordinates Forward (+)

19441023 .. 19447218

Gene Structure 6196 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug06G0137400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2532 bp

ATGGGAGCTAGCTCAGCTTTGAGCTGGGTTCTGCTCTGCTTAGTTGTGTTGTCTCTAAGTTTTCAGCTGATACTGTGCAATGTTAGCTATGACAGGAAGGCTCTTATCATTAATGGGCAGAGGAGAATTCTCTTTTCTGGGTCCATACACTATCCTAGAAGTACTCCTGAGATGTGGGAAGGTCTTATACAGAAGGCCAAAGATGGTGGGTTGGATGTTATAGACACCTATGTGTTTTGGAATCTTCATGAGCCATCTCCTGGCAATTACAATTTTGAAGGGAGAAATGATTTGGTTCGCTTCATTAAGTTAGTCCAAGAGGCACGGCTCTATGTGCATCTTCGCATTGGGCCGTACATTTGCTCAGAATGGAATTTTGGGGGATTTCCAGTTTGGCTTAAGTATGTTCCAGGCATTAGCTTCAGAACAGATAATGAGCCTTTCAAGTCAGCAATGAAAAAATTTACCAAGAAGATTGTTCAATTGATGATGCATGAAAAGCTATTTGAGTCCCAAGGTGGCCCCATTATCCTTTCTCAGATTGAGAATGAATATGAGCCAGAAAGCAAGCTTTTTGGTGCTGCTGGTCATGCATACATGACTTGGGCTGCAGAGATGGCTGTTGGAATGGGTACTGGTGTTCCATGGGTCATGTGCAAGGAAGAGGATGCCCCAGACCCAGTGATAAACACTTGCAATGGTTTTTATTGCGACTACTTTTCTCCAAACAAACCATATAAACCCACACTTTGGACTGAGGCTTGGACTGGCTGGTTTACAGAATTTGGTGGCCCAATTTACAAGCGACCTGTTGAAGATTTGGCATTTGGAGTTGCCCGATTCATTCAGAATGGAGGATCCTTCATAAATTATTACATGTACCATGGAGGAACAAACTTTGGAAGAACTGCAGGAGGTCCATTTATTACTACCAGTTATGACTATGATGCTCCCATTGACGAATATGGCTTGATCAGGCAACCTAAATATGGCCATTTAAAGGAACTCCATAAGGCTGTTAAGTTATGTGAGCAAGCTCTGCTGAACACTGATTCTACTGTTACATCCTTAGGGACTTATGAACAGGCACACGTATTCTCTTCCAAGTCAGGAGATTGTGCAGCTTTTCTCTCAAATTACAATACAAAGTCAGCTGCAACTGTGACTTTCAATCAGATGAACTATCACCTCCCACCTTGGTCCATCAGCATCTTGCCAGATTGTAAAAACGTTGTATACAACACTGCACAAGTGGGAGTTCAAACATCCCAAAAACAGTTTTTTCCTACTAACTCTAAGTTGCGCTCATGGGAAACCTTCAGTGAAGACATCTCTTCAGTTGCTAGCAATACGAAACTCACGGTAATTGGTCTCTTAGATCAGTTGAACATCACCAGAGACTCCAGTGATTACTTATGGTACACAACCAATATCGACATTAAATCATCTGAATCATTTCTGCGTGGCGGTCAACATCCAACTCTGACCGTGCAATCAGCTGGTGATGCTATGCATGTCTTTATCAATGGGCAGCTCGCAGGATCGGCGTATGGGACTAGGAAAAACAAGCGATTCACTTTTACAGGAAATGTCATCCTGCATGCTGGATTGAATAAAATCTCACTTCTCAGTATAGCTGTTGGATTGCCGAACAATGGTCCTCACTTTGAGACACGGAATGCTGGAGTACTGGGACCAGTTGCTCTACATGGACTAGACCAAGGAAGCAGAGACTTATCATGGCAGAAATGGTCACACAAGGTGGGCCTAAAAGGAGAAGCCATGAACCTGGGTTCTCCAAGTTCCATCTCGACAGTGGATTGGACGAGGGCGTCCTTGGTGGCGAAGAATCAACAGCCACTGACATGGTATAAGGTCAATTTTGATGCACCTGAAGGAGATGAACCTTTGGCTTTGGACATGGGAAGTATGGGCAAGGGTCAACTATGGATCAATGGGCAAAGCATCGGAAGATACTGGACTACTTATGCTAACGGGGATTGCAGTGCGTGCAGTTATTCTGGTACCTTCAGGCCTAAGAACAAGTGCCAATATGGTTGTAACCATCCAACTCAACAATGGTACCATGTTCCTAGGTCCTGGTTAAAGCAATCTAAAAACCTATTGGTAGTTTTTGTGGAGATTGGTGGGGATGTATCAAAGATTGCTCTTGTCAAACGTTCAGTGAGCACTGTTTGTGCTGAGGTTTCTGAAAATCACCCACATAATAGGAATTGGCATACTGAGAGCCGTGGCCAACTGGAAGAGCAAAAGAAGCCTATAATGAGCCTACACTGTACGGATGGATACTCCATTTCTGCTATTAAGTTTTCAAGTTTTGGAACTCCATCTGGAAGCTGTGGGAATTTTCAGCACGGTACATGTCACGCTCCAAACTCGAAGGCTGTCATTGAGAAGAAGTGTATAGGTAAGCAGAAATGTTCATTGACCATATCAGATGCCAATTTTGGGATGGATCCATGTCCAAGCATGCTGAAAAAATTATCTGTTGAAGCTGTTTGTGCCCCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005351 GO:0005355 GO:0005402 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0006810 GO:0006811 GO:0006812 GO:0008150 GO:0008324 GO:0008643 GO:0008645 GO:0012505 GO:0015075 GO:0015077 GO:0015078 GO:0015144 GO:0015145 GO:0015149 GO:0015291 GO:0015293 GO:0015294 GO:0015295 GO:0015318 GO:0015672 GO:0015749 GO:0016020 GO:0022804 GO:0022857 GO:0022890 GO:0031090 GO:0034219 GO:0034220 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046323 GO:0051119 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:1902600 GO:1904659

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Sugar_tr

Protein Analysis

843

Amino Acids

93.72

Weight (kDa)

8.46

Isoelectric Point (pI)

39.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_35	PF01301	35 - 339	1.6e-122	Glycosyl hydrolases family 35
GHD	PF17834	362 - 418	4.2e-25	Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd	PF21467	471 - 538	2.8e-08	Beta-galactosidase, galactose-binding domain
BetaGal_gal-bd	PF21467	621 - 707	5.3e-27	Beta-galactosidase, galactose-binding domain
SUEL_Lectin	PF02140	764 - 841	7.5e-21	D-galactoside/L-rhamnose binding SUEL lectin domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001036)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G35300 AT4G35300 AT4G35300 AT4G35300 AT4G35300 AT4G35300 AT4G35300 AT4G35300 AT4G35300 AT4G35300 AT4G35300
fragaria_vesca	FvH4_2g16850 FvH4_2g16850 FvH4_2g16850 FvH4_2g16850 FvH4_2g16850 FvH4_2g16850 FvH4_2g16850 FvH4_2g16850 FvH4_2g16850 FvH4_5g25950 FvH4_5g25950 FvH4_5g25950 FvH4_5g25950
malus_domestica	MD05G1139800.v1.1 MD06G1005100.v1.1 MD10G1142100.v1.1
prunus_persica	Prupe.5G006300_v2.0.a1 Prupe.5G006300_v2.0.a1 Prupe.5G006300_v2.0.a1 Prupe.5G006300_v2.0.a1 Prupe.5G006300_v2.0.a1 Prupe.5G006300_v2.0.a1 Prupe.8G180600_v2.0.a1 Prupe.8G180600_v2.0.a1 Prupe.8G180600_v2.0.a1 Prupe.8G180600_v2.0.a1
pyrus_communis	pycom05g13500 pycom10g12180
rosa_chinensis	RchiOBHm_Chr6g0280981 RchiOBHm_Chr7g0216501
rosa_laevigata	RLG00000002567 RLG00000002578 RLG00000013010
rosa_multiflora	Rmu_sc0000692.1_g000001 Rmu_sc0030247.1_g000002 Rmu_sc0030424.1_g000003 Rmu_sc0034915.1_g000001
rosa_roxburghii	Rroxscaffold_3G00243170 Rroxscaffold_7G00187430
rosa_rugosa	Rorug06G0137300 Rorug06G0137400 Rorug06G0137500 Rorug07G0160700
rosa_samantha	Rh6AG249900 Rh6CG252500 Rh7BG291500 Rh7DG301100
rosa_wichuraiana	Rw6G021600 Rw7G025360 Rw7G025500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1831
Acc65I	GGTACC	2 cut(s)	2024, 2082
AccB1I	GGYRCC	2 cut(s)	2024, 2082
AccB7I	CCANNNNNTGG	2 cut(s)	260, 750
AccI	GTMKAC	1 cut(s)	1236
AciI	CCGC	1 cut(s)	1467
AclI	AACGTT	2 cut(s)	1230, 2179
AclWI	GGATC	6 cut(s)	852, 865, 1549, 1958, 2471, 2484
AcoI	YGGCCR	2 cut(s)	991, 2254
AcsI	RAATTY	5 cut(s)	126, 373, 461, 782, 2365
AcuI	CTGAAG	5 cut(s)	406, 1303, 1320, 1914, 2014
AcyI	GRCGYC	1 cut(s)	1832
AdeI	CACNNNGTG	1 cut(s)	1762
AfiI	CCNNNNNNNGG	6 cut(s)	260, 750, 989, 1000, 1655, 2469
AflII	CTTAAG	1 cut(s)	398
AflIII	ACRYGT	3 cut(s)	876, 1090, 2381
AjnI	CCWGG	4 cut(s)	259, 409, 1788, 2099
AjuI	GAANNNNNNNTTGG	2 cut(s)	360, 392
AleI	CACNNNNGTG	1 cut(s)	230
Alw21I	GWGCWC	1 cut(s)	2192
Alw26I	GTCTC	5 cut(s)	57, 1376, 1392, 1664, 1725
AlwI	GGATC	6 cut(s)	852, 865, 1549, 1958, 2471, 2484
AlwNI	CAGNNNCTG	1 cut(s)	1861
AoxI	GGCC	8 cut(s)	195, 350, 520, 790, 991, 1765, 2033, 2254
ApeKI	GCWGC	5 cut(s)	581, 608, 1121, 1154, 1531
ApoI	RAATTY	5 cut(s)	126, 373, 461, 782, 2365
AseI	ATTAAT	1 cut(s)	111
Asp700I	GAANNNNTTC	1 cut(s)	1455
Asp718I	GGTACC	2 cut(s)	2024, 2082
AspA2I	CCTAGG	1 cut(s)	2093
AspLEI	GCGC	1 cut(s)	1305
AsuHPI	GGTGA	4 cut(s)	1178, 1384, 1514, 2210
AsuNHI	GCTAGC	2 cut(s)	8, 1343
AvaII	GGWCC	6 cut(s)	141, 917, 1200, 1658, 1694, 2097
AvrII	CCTAGG	1 cut(s)	2093
AxyI	CCTNAGG	1 cut(s)	1069
BaeGI	GKGCMC	1 cut(s)	2527
BaeI	ACNNNNGTAYC	4 cut(s)	624, 657, 2066, 2099
BalI	TGGCCA	2 cut(s)	993, 2256
BamHI	GGATCC	2 cut(s)	857, 2476
BanI	GGYRCC	2 cut(s)	2024, 2082
BarI	GAAGNNNNNNTAC	2 cut(s)	171, 203
BbsI	GAAGAC	1 cut(s)	1332
Bbv12I	GWGCWC	1 cut(s)	2192
BbvCI	CCTCAGC	1 cut(s)	2202
BbvI	GCAGC	5 cut(s)	568, 595, 1133, 1141, 1543
BccI	CCATC	9 cut(s)	197, 262, 610, 1211, 1814, 2073, 2297, 2356, 2467
BceAI	ACGGC	3 cut(s)	337, 341, 2235
BciT130I	CCWGG	4 cut(s)	261, 411, 1790, 2101
BciVI	GTATCC	1 cut(s)	2300
BclI	TGATCA	1 cut(s)	972
BcoDI	GTCTC	5 cut(s)	57, 1376, 1392, 1664, 1725
BfaI	CTAG	6 cut(s)	9, 156, 1344, 1557, 1715, 2094
BfmI	CTRYAG	2 cut(s)	609, 909
BfrI	CTTAAG	1 cut(s)	398
BfuI	GTATCC	1 cut(s)	2300
BisI	GCNGC	5 cut(s)	582, 609, 1122, 1155, 1532
BlnI	CCTAGG	1 cut(s)	2093
BlpI	GCTNAGC	1 cut(s)	13
BlsI	GCNGC	5 cut(s)	583, 610, 1123, 1156, 1533
BmcAI	AGTACT	2 cut(s)	163, 1689
Bme1390I	CCNGG	4 cut(s)	261, 411, 1790, 2101
Bme18I	GGWCC	6 cut(s)	141, 917, 1200, 1658, 1694, 2097
BmiI	GGNNCC	7 cut(s)	142, 523, 859, 1695, 2026, 2084, 2478
BmrFI	CCNGG	4 cut(s)	261, 411, 1790, 2101
BmrI	ACTGGG	2 cut(s)	674, 1700
BmsI	GCATC	9 cut(s)	346, 480, 658, 937, 1218, 1495, 1876, 1974, 2449
BmtI	GCTAGC	2 cut(s)	12, 1347
BmuI	ACTGGG	2 cut(s)	674, 1700
BpiI	GAAGAC	1 cut(s)	1332
BpmI	CTGGAG	2 cut(s)	1387, 1704
Bpu10I	CCTNAGC	1 cut(s)	2202
Bpu1102I	GCTNAGC	1 cut(s)	13
BsaAI	YACGTR	1 cut(s)	1093
BsaHI	GRCGYC	1 cut(s)	1832
BsaI	GGTCTC	1 cut(s)	1376
BsaJI	CCNNGG	9 cut(s)	514, 644, 883, 1196, 1720, 1789, 1836, 2093, 2251
Bsc4I	CCNNNNNNNGG	6 cut(s)	260, 750, 989, 1000, 1655, 2469
Bse21I	CCTNAGG	1 cut(s)	1069
Bse3DI	GCAATG	3 cut(s)	85, 459, 703
BseBI	CCWGG	4 cut(s)	261, 411, 1790, 2101
BseDI	CCNNGG	9 cut(s)	514, 644, 883, 1196, 1720, 1789, 1836, 2093, 2251
BseLI	CCNNNNNNNGG	6 cut(s)	260, 750, 989, 1000, 1655, 2469
BseMI	GCAATG	3 cut(s)	85, 459, 703
BseMII	CTCAG	8 cut(s)	27, 159, 378, 551, 747, 1642, 2193, 2235
BseRI	GAGGAG	1 cut(s)	136
BseSI	GKGCMC	1 cut(s)	2527
BseXI	GCAGC	5 cut(s)	568, 595, 1133, 1141, 1543
BseYI	CCCAGC	1 cut(s)	24
BsgI	GTGCAG	3 cut(s)	1140, 1230, 2032
BshFI	GGCC	8 cut(s)	197, 352, 522, 792, 993, 1767, 2035, 2256
BshNI	GGYRCC	2 cut(s)	2024, 2082
BsiHKAI	GWGCWC	1 cut(s)	2192
BslFI	GGGAC	4 cut(s)	496, 1087, 1567, 1707
BslI	CCNNNNNNNGG	6 cut(s)	260, 750, 989, 1000, 1655, 2469
BsmAI	GTCTC	5 cut(s)	57, 1376, 1392, 1664, 1725
BsmFI	GGGAC	4 cut(s)	496, 1087, 1567, 1707
BsmI	GAATGC	1 cut(s)	1684
BsnI	GGCC	8 cut(s)	197, 352, 522, 792, 993, 1767, 2035, 2256
Bso31I	GGTCTC	1 cut(s)	1376
Bsp1286I	GDGCHC	2 cut(s)	2192, 2527
Bsp143I	GATC	6 cut(s)	857, 972, 1378, 1541, 1950, 2476
Bsp1720I	GCTNAGC	1 cut(s)	13
Bsp19I	CCATGG	2 cut(s)	644, 883
BspACI	CCGC	1 cut(s)	1467
BspANI	GGCC	8 cut(s)	197, 352, 522, 792, 993, 1767, 2035, 2256
BspCNI	CTCAG	8 cut(s)	26, 160, 377, 550, 748, 1641, 2194, 2236
BspHI	TCATGA	1 cut(s)	247
BspLI	GGNNCC	7 cut(s)	142, 523, 859, 1695, 2026, 2084, 2478
BspMAI	CTGCAG	2 cut(s)	613, 913
BspOI	GCTAGC	2 cut(s)	12, 1347
BspPI	GGATC	6 cut(s)	852, 865, 1549, 1958, 2471, 2484
BspQI	GCTCTTC	1 cut(s)	2259
BspT107I	GGYRCC	2 cut(s)	2024, 2082
BspTI	CTTAAG	1 cut(s)	398
BspTNI	GGTCTC	1 cut(s)	1376
BsrDI	GCAATG	3 cut(s)	85, 459, 703
BssECI	CCNNGG	9 cut(s)	514, 644, 883, 1196, 1720, 1789, 1836, 2093, 2251
BssMI	GATC	6 cut(s)	857, 972, 1378, 1541, 1950, 2476
BssNAI	GTATAC	1 cut(s)	1237
BssNI	GRCGYC	1 cut(s)	1832
BssT1I	CCWWGG	7 cut(s)	514, 644, 883, 1196, 1720, 1836, 2093
Bst1107I	GTATAC	1 cut(s)	1237
Bst2UI	CCWGG	4 cut(s)	261, 411, 1790, 2101
Bst6I	CTCTTC	4 cut(s)	657, 1105, 1339, 2259
BstACI	GRCGYC	1 cut(s)	1832
BstAFI	CTTAAG	1 cut(s)	398
BstAPI	GCANNNNNTGC	2 cut(s)	1039, 2010
BstBAI	YACGTR	1 cut(s)	1093
BstC8I	GCNNGC	8 cut(s)	10, 569, 1035, 1345, 1536, 1602, 2011, 2492
BstDSI	CCRYGG	3 cut(s)	644, 883, 2251
BstHHI	GCGC	1 cut(s)	1305
BstKTI	GATC	6 cut(s)	860, 975, 1381, 1544, 1953, 2479
BstMAI	GTCTC	5 cut(s)	57, 1376, 1392, 1664, 1725
BstMBI	GATC	6 cut(s)	857, 972, 1378, 1541, 1950, 2476
BstNI	CCWGG	4 cut(s)	261, 411, 1790, 2101
BstNSI	RCATGY	5 cut(s)	880, 1517, 1604, 2385, 2494
BstSCI	CCNGG	4 cut(s)	259, 409, 1788, 2099
BstSFI	CTRYAG	2 cut(s)	609, 909
BstSLI	GKGCMC	1 cut(s)	2527
BstV1I	GCAGC	5 cut(s)	568, 595, 1133, 1141, 1543
BstV2I	GAAGAC	1 cut(s)	1332
BstX2I	RGATCY	2 cut(s)	857, 2476
BstXI	CCANNNNNNTGG	2 cut(s)	1789, 1866
BstYI	RGATCY	2 cut(s)	857, 2476
BstZ17I	GTATAC	1 cut(s)	1237
Bsu36I	CCTNAGG	1 cut(s)	1069
BsuI	GTATCC	1 cut(s)	2300
BsuRI	GGCC	8 cut(s)	197, 352, 522, 792, 993, 1767, 2035, 2256
BtgI	CCRYGG	3 cut(s)	644, 883, 2251
BtsI	GCAGTG	2 cut(s)	1242, 2011
Cac8I	GCNNGC	8 cut(s)	10, 569, 1035, 1345, 1536, 1602, 2011, 2492
CaiI	CAGNNNCTG	1 cut(s)	1861
CciI	TCATGA	1 cut(s)	247
CfoI	GCGC	1 cut(s)	1305
CseI	GACGC	1 cut(s)	1821
CspCI	CAANNNNNGTGG	2 cut(s)	187, 222
DpnI	GATC	6 cut(s)	859, 974, 1380, 1543, 1952, 2478
DpnII	GATC	6 cut(s)	857, 972, 1378, 1541, 1950, 2476
DraI	TTTAAA	1 cut(s)	999
DraIII	CACNNNGTG	1 cut(s)	1762
DrdI	GACNNNNNNGTC	1 cut(s)	1831
DseDI	GACNNNNNNGTC	1 cut(s)	1831
EaeI	YGGCCR	2 cut(s)	991, 2254
Eam1104I	CTCTTC	4 cut(s)	657, 1105, 1339, 2259
EarI	CTCTTC	4 cut(s)	657, 1105, 1339, 2259
Eco130I	CCWWGG	7 cut(s)	514, 644, 883, 1196, 1720, 1836, 2093
Eco147I	AGGCCT	1 cut(s)	2035
Eco31I	GGTCTC	1 cut(s)	1376
Eco47I	GGWCC	6 cut(s)	141, 917, 1200, 1658, 1694, 2097
Eco57I	CTGAAG	5 cut(s)	406, 1303, 1320, 1914, 2014
Eco81I	CCTNAGG	1 cut(s)	1069
EcoO109I	RGGNCCY	1 cut(s)	2097
EcoRI	GAATTC	1 cut(s)	126
EcoRII	CCWGG	4 cut(s)	259, 409, 1788, 2099
EcoT14I	CCWWGG	7 cut(s)	514, 644, 883, 1196, 1720, 1836, 2093
EcoT22I	ATGCAT	3 cut(s)	495, 595, 1515
ErhI	CCWWGG	7 cut(s)	514, 644, 883, 1196, 1720, 1836, 2093
FaqI	GGGAC	4 cut(s)	496, 1087, 1567, 1707
FbaI	TGATCA	1 cut(s)	972
FblI	GTMKAC	1 cut(s)	1236
Fnu4HI	GCNGC	5 cut(s)	582, 609, 1122, 1155, 1532
Fsp4HI	GCNGC	5 cut(s)	582, 609, 1122, 1155, 1532
FspBI	CTAG	6 cut(s)	9, 156, 1344, 1557, 1715, 2094
GlaI	GCGC	1 cut(s)	1304
GluI	GCNGC	5 cut(s)	582, 609, 1122, 1155, 1532
GsaI	CCCAGC	1 cut(s)	28
GsuI	CTGGAG	2 cut(s)	1387, 1704
HaeIII	GGCC	8 cut(s)	197, 352, 522, 792, 993, 1767, 2035, 2256
HgaI	GACGC	1 cut(s)	1821
HhaI	GCGC	1 cut(s)	1305
Hin1I	GRCGYC	1 cut(s)	1832
Hin6I	GCGC	1 cut(s)	1303
HinP1I	GCGC	1 cut(s)	1303
HincII	GTYRAC	2 cut(s)	1472, 1943
HindII	GTYRAC	2 cut(s)	1472, 1943
HindIII	AAGCTT	1 cut(s)	569
HinfI	GANTC	8 cut(s)	241, 509, 840, 1052, 1400, 1451, 1572, 1849
HphI	GGTGA	4 cut(s)	1178, 1384, 1514, 2210
Hpy166II	GTNNAC	5 cut(s)	777, 1237, 1422, 1472, 1943
Hpy188III	TCNNGA	5 cut(s)	167, 248, 1110, 1810, 2352
Hpy8I	GTNNAC	5 cut(s)	777, 1237, 1422, 1472, 1943
HpyAV	CCTTC	9 cut(s)	91, 173, 187, 272, 871, 1327, 1889, 2038, 2395
HpyCH4IV	ACGT	3 cut(s)	1092, 1230, 2179
HpySE526I	ACGT	3 cut(s)	1092, 1230, 2179
Hsp92I	GRCGYC	1 cut(s)	1832
HspAI	GCGC	1 cut(s)	1303
KpnI	GGTACC	2 cut(s)	2028, 2086
Ksp22I	TGATCA	1 cut(s)	972
Kzo9I	GATC	6 cut(s)	857, 972, 1378, 1541, 1950, 2476
LguI	GCTCTTC	1 cut(s)	2259
LmnI	GCTCC	3 cut(s)	5, 955, 2395
Lsp1109I	GCAGC	5 cut(s)	568, 595, 1133, 1141, 1543
LweI	GCATC	9 cut(s)	346, 480, 658, 937, 1218, 1495, 1876, 1974, 2449
MaeI	CTAG	6 cut(s)	9, 156, 1344, 1557, 1715, 2094
MaeII	ACGT	3 cut(s)	1092, 1230, 2179
MaeIII	GTNAC	5 cut(s)	1060, 1162, 1752, 2060, 2384
MalI	GATC	6 cut(s)	859, 974, 1380, 1543, 1952, 2478
MboI	GATC	6 cut(s)	857, 972, 1378, 1541, 1950, 2476
MfeI	CAATTG	1 cut(s)	482
MflI	RGATCY	2 cut(s)	857, 2476
MhlI	GDGCHC	2 cut(s)	2192, 2527
MlsI	TGGCCA	2 cut(s)	993, 2256
MluNI	TGGCCA	2 cut(s)	993, 2256
MlyI	GAGTC	2 cut(s)	518, 1394
MmeI	TCCRAC	5 cut(s)	192, 604, 1502, 1621, 2093
Mox20I	TGGCCA	2 cut(s)	993, 2256
Mph1103I	ATGCAT	3 cut(s)	495, 595, 1515
MroXI	GAANNNNTTC	1 cut(s)	1455
MscI	TGGCCA	2 cut(s)	993, 2256
MseI	TTAA	8 cut(s)	111, 306, 399, 998, 1020, 1440, 2105, 2325
MslI	CAYNNNNRTG	2 cut(s)	230, 1864
Msp20I	TGGCCA	2 cut(s)	993, 2256
MspA1I	CMGCKG	3 cut(s)	67, 1154, 1499
MspCI	CTTAAG	1 cut(s)	398
MspR9I	CCNGG	4 cut(s)	261, 411, 1790, 2101
MunI	CAATTG	1 cut(s)	482
Mva1269I	GAATGC	1 cut(s)	1684
MvaI	CCWGG	4 cut(s)	261, 411, 1790, 2101
NcoI	CCATGG	2 cut(s)	644, 883
NdeII	GATC	6 cut(s)	857, 972, 1378, 1541, 1950, 2476
NheI	GCTAGC	2 cut(s)	8, 1343
NlaIV	GGNNCC	7 cut(s)	142, 523, 859, 1695, 2026, 2084, 2478
NmuCI	GTSAC	3 cut(s)	1162, 1752, 2384
NsiI	ATGCAT	3 cut(s)	495, 595, 1515
NspI	RCATGY	5 cut(s)	880, 1517, 1604, 2385, 2494
OliI	CACNNNNGTG	1 cut(s)	230
PaeI	GCATGC	2 cut(s)	1604, 2494
PagI	TCATGA	1 cut(s)	247
PceI	AGGCCT	1 cut(s)	2035
PciI	ACATGT	2 cut(s)	876, 2381
PciSI	GCTCTTC	1 cut(s)	2259
PctI	GAATGC	1 cut(s)	1684
PdmI	GAANNNNTTC	1 cut(s)	1455
PfeI	GAWTC	6 cut(s)	241, 840, 1052, 1451, 1572, 1849
PflMI	CCANNNNNTGG	2 cut(s)	260, 750
PkrI	GCNGC	5 cut(s)	583, 610, 1123, 1156, 1533
PleI	GAGTC	2 cut(s)	517, 1394
PpsI	GAGTC	2 cut(s)	517, 1394
Ppu21I	YACGTR	1 cut(s)	1093
PpuMI	RGGWCCY	1 cut(s)	2097
PscI	ACATGT	2 cut(s)	876, 2381
PshBI	ATTAAT	1 cut(s)	111
Psp1406I	AACGTT	2 cut(s)	1230, 2179
Psp5II	RGGWCCY	1 cut(s)	2097
Psp6I	CCWGG	4 cut(s)	259, 409, 1788, 2099
PspFI	CCCAGC	1 cut(s)	24
PspGI	CCWGG	4 cut(s)	259, 409, 1788, 2099
PspN4I	GGNNCC	7 cut(s)	142, 523, 859, 1695, 2026, 2084, 2478
PspPPI	RGGWCCY	1 cut(s)	2097
PstI	CTGCAG	2 cut(s)	613, 913
PstNI	CAGNNNCTG	1 cut(s)	1861
PsuI	RGATCY	2 cut(s)	857, 2476
PvuII	CAGCTG	3 cut(s)	67, 1154, 1499
RseI	CAYNNNNRTG	2 cut(s)	230, 1864
SapI	GCTCTTC	1 cut(s)	2259
SaqAI	TTAA	8 cut(s)	111, 306, 399, 998, 1020, 1440, 2105, 2325
SatI	GCNGC	5 cut(s)	582, 609, 1122, 1155, 1532
Sau3AI	GATC	6 cut(s)	857, 972, 1378, 1541, 1950, 2476
ScaI	AGTACT	2 cut(s)	163, 1689
SchI	GAGTC	2 cut(s)	518, 1394
ScrFI	CCNGG	4 cut(s)	261, 411, 1790, 2101
SduI	GDGCHC	2 cut(s)	2192, 2527
SfaNI	GCATC	9 cut(s)	346, 480, 658, 937, 1218, 1495, 1876, 1974, 2449
SfcI	CTRYAG	2 cut(s)	609, 909
SinI	GGWCC	6 cut(s)	141, 917, 1200, 1658, 1694, 2097
SmiMI	CAYNNNNRTG	2 cut(s)	230, 1864
SmlI	CTYRAG	1 cut(s)	398
SmoI	CTYRAG	1 cut(s)	398
SphI	GCATGC	2 cut(s)	1604, 2494
SseBI	AGGCCT	1 cut(s)	2035
SsiI	CCGC	1 cut(s)	1467
SspMI	CTAG	6 cut(s)	9, 156, 1344, 1557, 1715, 2094
StuI	AGGCCT	1 cut(s)	2035
StyD4I	CCNGG	4 cut(s)	259, 409, 1788, 2099
StyI	CCWWGG	7 cut(s)	514, 644, 883, 1196, 1720, 1836, 2093
TaiI	ACGT	3 cut(s)	1095, 1233, 2182
TaqI	TCGA	3 cut(s)	1434, 1811, 2399
TatI	WGTACW	2 cut(s)	161, 1687
TfiI	GAWTC	6 cut(s)	241, 840, 1052, 1451, 1572, 1849
Tru1I	TTAA	8 cut(s)	111, 306, 399, 998, 1020, 1440, 2105, 2325
Tru9I	TTAA	8 cut(s)	111, 306, 399, 998, 1020, 1440, 2105, 2325
TseFI	GTSAC	3 cut(s)	1162, 1752, 2384
TseI	GCWGC	5 cut(s)	581, 608, 1121, 1154, 1531
Tsp45I	GTSAC	3 cut(s)	1162, 1752, 2384
TspGWI	ACGGA	2 cut(s)	1690, 2315
Van91I	CCANNNNNTGG	2 cut(s)	260, 750
Vha464I	CTTAAG	1 cut(s)	398
VpaK11BI	GGWCC	6 cut(s)	141, 917, 1200, 1658, 1694, 2097
VspI	ATTAAT	1 cut(s)	111
XapI	RAATTY	5 cut(s)	126, 373, 461, 782, 2365
XceI	RCATGY	5 cut(s)	880, 1517, 1604, 2385, 2494
XcmI	CCANNNNNNNNNTGG	3 cut(s)	1813, 2076, 2470
XmaJI	CCTAGG	1 cut(s)	2093
XmiI	GTMKAC	1 cut(s)	1236
XmnI	GAANNNNTTC	1 cut(s)	1455
XspI	CTAG	6 cut(s)	9, 156, 1344, 1557, 1715, 2094
ZrmI	AGTACT	2 cut(s)	163, 1689
Zsp2I	ATGCAT	3 cut(s)	495, 595, 1515

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.