Rorug07G0115200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0015995
Physical Location & Seq
Reverse (-)
8970653 .. 8972566
1914 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0115200.1

Sequence Viewer

Length: 1914 bp
ATGGACCCTTTTCCCGGCAAACCGCAGCTCCTCCGCCTCCTCACCTTCCTCTCCCTCCTCCTCCTCATCCACGGCGGTAGGCTCAACCTCGACCCTTCAGACCTTCAAGCCCTCATCACTGTCCAAACCCACCTCGGCTTCCACACCCCAGACAGAGCCAAACCTACCAACCCATGCAACACCCCCGGCGTCTTCTGCGAGAGAAGACTCTCCAACAACACCTACGTCCTCAAAATCACTAGACTCGTCTTCAAGTCCCAACTCCTCTCCGGCTCTCTGTCTCCGGCAATCGGACAGCTCTCCGAGCTTAAAGAGCTCTCTCTTCCGGACAACAGCCTCGTTGACCGAGTCCCGCCCGAAATCGTTGACTGCAAGAAGCTGGAGATCCTCAACCTCGGAAACAACCAGTTTTCGGGTGAAGTTCCTTCTCAGCTCTCTTCTCTAATCAGGCTTCGAATACTCGACCTCTCCTCCAACAAGTTCTCCGGCAACTTGAGCTTCTTGAAGCATTTTCCCAACTTGGAAAATCTCTCCATTGCCGACAATCTCTTCTCCGGGAAGATCCCAGCTTCGGTTCGTTCTTTTCGCAATCTCAGGTTCTTCAACTTCGCCGGAAATGAGTTCCTGGAAGGCTCCGCACCTAAGTTGAAAAGGGTCGAGGTTTCAGCTTCTTCCGAGGTTCCAAGGCGTTTCATTTTTGCTGAAACATCAAACTCCACCACCGCAGCAAAAACCAATAATAGTAATTCAATCGCTCAAGCACCCTCCGGTTCTAATGCTCAAGCTCCAGGTCCATCTGCAGCACCGAAGAAGAAGAAGAAGAGCAAGACGAAGAAACTCGGAGGCTGGCTGCTCGGGTTCTTCGCCGGAGCGCTGGCCGGGAGCATATCCGGGTTCATCTTCTCTCTGCTGTTCAAGCTGCTCTTGGCTGTGGTTAGAGGTGGAGGCAAGGACTCTGGTCCGGCCATTTTCAGTTCTCTGATTAAGAGAAAAGAGGACTTGGCTTTTCTGGAGAAAGAAGACGGGCTTACTTCGTTGGAGCTCATCGGAAGTGGAGGATGTGGACAGGTTTACAAAGCTGAGTTGCCAGGAAGCAACGGGAAGATAATCGCTATTAAGAAGATTATTCAACCTCCTAAGGAAGCGGCAGAGCTGACGGACGAAGACAGCAAGCAGATGCATAAGAAGATGCGTCAAATTCGGTCGGAGATCAATACCGTGGGACAAATTCGACACCGGAATCTGCTTCCTCTGTTGGCACATGTGTCCCGGCCGGACTGTCATCTGCTGGTGTATGAGTACATGAAAAATGGGAGCTTACAAGACATGTTGATTGAGGTTTCTGAAGGGAGAAAGGAACTGGATTGGTTAACAAGGCACAGGATTGCAATAGGAATCGCTTCAGGGCTTGAATATCTCCATGTGAACCATACACCTCACATCATTCACAGAGATCTGAAGCCGGCAAACGTCCTGCTTGATGACGATATGGAAGCTCGAATTGCAGATTTTGGGCTTGCGAAAGCAATGCCTGACTACCAGACGCATATCACGACTTCAAATGTGGCAGGAACGGTTGGTTACATTGCACCAGAGTATCATCAGACACTCAAGTTCACAGACAAGTGTGATATATACAGCTTCGGGGTATTGCTCGGGGTACTGATCATAGGAAAGCTACCGTCTGATGAATTTTTCCAGACCACCAGCGAGATGAGTCTGGTTAAGTGGATGAAGAATGTGCTGACGTCGGAGGATCCTAAGCAGGCAATCGACTCAAAATTGATGGGAAATGGATTTGAGGACCAAATGCTCTTGGTTCTAAAGATAGCATGCTTTTGCACAGTGGATAACCCGAAAGAGAGACCTAATAGTAAGGATGTTAGGTGCATGTTGGATCAAATCAAGCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

637

Amino Acids

70.28

Weight (kDa)

9.13

Isoelectric Point (pI)

36.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At5g56370 PF24758 101 - 223 3.9e-06 FBD-associated F-box protein At5g56370, LRR repeats
LRR_8 PF13855 102 - 161 7.5e-07 Leucine rich repeat
Pkinase PF00069 345 - 626 1.1e-41 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 345 - 584 1.8e-36 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015995)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G46795
fragaria_vesca FvH4_5g22030
prunus_persica Prupe.5G036900_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0209671
rosa_laevigata RLG00000002967 RLG00000003105
rosa_roxburghii Rroxscaffold_3G00249160
rosa_rugosa Rorug07G0115200 Rorug07G0115300
rosa_samantha Rh5BG034200 Rh7AG248900 Rh7BG242900 Rh7CG264800 Rh7DG254800
rosa_wichuraiana Rw7G021110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1751
AccIII TCCGGA 1 cut(s) 325
AciI CCGC 7 cut(s) 23, 34, 75, 353, 636, 723, 1145
AclWI GGATC 5 cut(s) 379, 556, 1751, 1764, 1905
AcoI YGGCCR 3 cut(s) 876, 963, 1271
AcsI RAATTY 3 cut(s) 1197, 1227, 1691
AcuI CTGAAG 4 cut(s) 81, 1365, 1386, 1478
AcyI GRCGYC 2 cut(s) 189, 1748
AfaI GTAC 2 cut(s) 1301, 1662
AfeI AGCGCT 1 cut(s) 873
AfiI CCNNNNNNNGG 4 cut(s) 14, 290, 412, 571
AflIII ACRYGT 2 cut(s) 1261, 1326
AjnI CCWGG 3 cut(s) 624, 787, 1087
Alw21I GWGCWC 2 cut(s) 318, 1044
Alw26I GTCTC 2 cut(s) 285, 1858
AlwI GGATC 5 cut(s) 379, 556, 1751, 1764, 1905
Ama87I CYCGRG 2 cut(s) 854, 1655
Aor13HI TCCGGA 1 cut(s) 325
Aor51HI AGCGCT 1 cut(s) 873
AoxI GGCC 3 cut(s) 876, 963, 1271
ApeKI GCWGC 5 cut(s) 25, 725, 800, 850, 919
ApoI RAATTY 3 cut(s) 1197, 1227, 1691
Asp700I GAANNNNTTC 1 cut(s) 1399
AspLEI GCGC 1 cut(s) 874
AspS9I GGNCC 4 cut(s) 4, 791, 959, 1804
AsuC2I CCSGG 6 cut(s) 15, 186, 556, 880, 892, 1270
AsuHPI GGTGA 2 cut(s) 34, 428
AsuII TTCGAA 1 cut(s) 454
AvaI CYCGRG 2 cut(s) 854, 1655
AvaII GGWCC 4 cut(s) 4, 791, 959, 1804
AxyI CCTNAGG 1 cut(s) 1137
BaeI ACNNNNGTAYC 2 cut(s) 1580, 1613
BamHI GGATCC 1 cut(s) 1756
BanII GRGCYC 2 cut(s) 318, 1044
BbsI GAAGAC 5 cut(s) 184, 211, 241, 1026, 1170
Bbv12I GWGCWC 2 cut(s) 318, 1044
BbvI GCAGC 5 cut(s) 37, 737, 812, 837, 906
BccI CCATC 2 cut(s) 802, 1780
BceAI ACGGC 1 cut(s) 88
BcgI CGANNNNNNTGC 2 cut(s) 1510, 1544
BciT130I CCWGG 3 cut(s) 626, 789, 1089
BclI TGATCA 1 cut(s) 1665
BcnI CCSGG 6 cut(s) 15, 186, 556, 880, 892, 1270
BcoDI GTCTC 2 cut(s) 285, 1858
BfaI CTAG 1 cut(s) 240
BfmI CTRYAG 1 cut(s) 798
BfoI RGCGCY 1 cut(s) 875
BglII AGATCT 1 cut(s) 1453
BisI GCNGC 6 cut(s) 26, 726, 801, 851, 920, 1146
BlsI GCNGC 6 cut(s) 27, 727, 802, 852, 921, 1147
Bme1390I CCNGG 9 cut(s) 15, 186, 556, 626, 789, 880, 892, 1089, 1270
Bme18I GGWCC 4 cut(s) 4, 791, 959, 1804
BmeT110I CYCGRG 2 cut(s) 854, 1655
BmgT120I GGNCC 4 cut(s) 4, 791, 959, 1804
BmiI GGNNCC 4 cut(s) 6, 634, 681, 1758
BmrFI CCNGG 9 cut(s) 15, 186, 556, 626, 789, 880, 892, 1089, 1270
BmsI GCATC 2 cut(s) 1167, 1179
BoxI GACNNNNGTC 1 cut(s) 957
BpiI GAAGAC 5 cut(s) 184, 211, 241, 1026, 1170
BpmI CTGGAG 3 cut(s) 401, 771, 1031
Bpu10I CCTNAGC 1 cut(s) 1761
Bpu14I TTCGAA 1 cut(s) 454
BpuEI CTTGAG 4 cut(s) 514, 741, 765, 1595
BpuMI CCSGG 6 cut(s) 15, 186, 556, 880, 892, 1270
BsaHI GRCGYC 2 cut(s) 189, 1748
BsaI GGTCTC 1 cut(s) 1858
BsaJI CCNNGG 7 cut(s) 70, 133, 184, 394, 675, 683, 1218
BsaWI WCCGGW 3 cut(s) 325, 767, 1236
BsaXI ACNNNNNCTCC 6 cut(s) 12, 42, 455, 467, 485, 497
Bsc4I CCNNNNNNNGG 4 cut(s) 14, 290, 412, 571
Bse118I RCCGGY 1 cut(s) 1462
Bse1I ACTGG 2 cut(s) 406, 1365
Bse21I CCTNAGG 1 cut(s) 1137
Bse3DI GCAATG 3 cut(s) 534, 1533, 1584
BseAI TCCGGA 1 cut(s) 325
BseBI CCWGG 3 cut(s) 626, 789, 1089
BseDI CCNNGG 7 cut(s) 70, 133, 184, 394, 675, 683, 1218
BseGI GGATG 4 cut(s) 66, 1064, 1737, 1885
BseLI CCNNNNNNNGG 4 cut(s) 14, 290, 412, 571
BseMI GCAATG 3 cut(s) 534, 1533, 1584
BseMII CTCAG 3 cut(s) 443, 607, 1071
BseNI ACTGG 2 cut(s) 406, 1365
BseRI GAGGAG 7 cut(s) 20, 29, 47, 50, 53, 254, 460
BseX3I CGGCCG 1 cut(s) 1271
BseXI GCAGC 5 cut(s) 37, 737, 812, 837, 906
BseYI CCCAGC 1 cut(s) 565
Bsh1285I CGRYCG 2 cut(s) 1205, 1274
BshFI GGCC 3 cut(s) 878, 965, 1273
BsiEI CGRYCG 2 cut(s) 1205, 1274
BsiHKAI GWGCWC 2 cut(s) 318, 1044
BsiHKCI CYCGRG 2 cut(s) 854, 1655
BslFI GGGAC 4 cut(s) 241, 335, 1236, 1252
BslI CCNNNNNNNGG 4 cut(s) 14, 290, 412, 571
BsmAI GTCTC 2 cut(s) 285, 1858
BsmFI GGGAC 4 cut(s) 241, 335, 1236, 1252
BsnI GGCC 3 cut(s) 878, 965, 1273
Bso31I GGTCTC 1 cut(s) 1858
BsoBI CYCGRG 2 cut(s) 854, 1655
Bsp119I TTCGAA 1 cut(s) 454
Bsp1286I GDGCHC 2 cut(s) 318, 1044
Bsp13I TCCGGA 1 cut(s) 325
Bsp143I GATC 7 cut(s) 384, 561, 1209, 1453, 1665, 1756, 1897
BspACI CCGC 7 cut(s) 23, 34, 75, 353, 636, 723, 1145
BspANI GGCC 3 cut(s) 878, 965, 1273
BspCNI CTCAG 3 cut(s) 442, 606, 1072
BspEI TCCGGA 1 cut(s) 325
BspLI GGNNCC 4 cut(s) 6, 634, 681, 1758
BspMAI CTGCAG 1 cut(s) 802
BspPI GGATC 5 cut(s) 379, 556, 1751, 1764, 1905
BspQI GCTCTTC 1 cut(s) 815
BspT104I TTCGAA 1 cut(s) 454
BspTNI GGTCTC 1 cut(s) 1858
BsrDI GCAATG 3 cut(s) 534, 1533, 1584
BsrFI RCCGGY 1 cut(s) 1462
BsrI ACTGG 2 cut(s) 406, 1365
BssAI RCCGGY 1 cut(s) 1462
BssECI CCNNGG 7 cut(s) 70, 133, 184, 394, 675, 683, 1218
BssMI GATC 7 cut(s) 384, 561, 1209, 1453, 1665, 1756, 1897
BssNI GRCGYC 2 cut(s) 189, 1748
BssT1I CCWWGG 1 cut(s) 683
Bst2UI CCWGG 3 cut(s) 626, 789, 1089
Bst4CI ACNGT 6 cut(s) 121, 1219, 1280, 1576, 1683, 1846
Bst6I CTCTTC 4 cut(s) 327, 442, 554, 815
BstACI GRCGYC 2 cut(s) 189, 1748
BstBI TTCGAA 1 cut(s) 454
BstC8I GCNNGC 7 cut(s) 848, 876, 1172, 1464, 1518, 1767, 1834
BstDEI CTNAG 6 cut(s) 429, 593, 642, 1080, 1137, 1761
BstDSI CCRYGG 2 cut(s) 70, 1218
BstF5I GGATG 4 cut(s) 66, 1064, 1737, 1885
BstH2I RGCGCY 1 cut(s) 875
BstHHI GCGC 1 cut(s) 874
BstKTI GATC 7 cut(s) 387, 564, 1212, 1456, 1668, 1759, 1900
BstMAI GTCTC 2 cut(s) 285, 1858
BstMBI GATC 7 cut(s) 384, 561, 1209, 1453, 1665, 1756, 1897
BstMCI CGRYCG 2 cut(s) 1205, 1274
BstMWI GCNNNNNNNGC 6 cut(s) 195, 304, 313, 495, 916, 1502
BstNI CCWGG 3 cut(s) 626, 789, 1089
BstNSI RCATGY 4 cut(s) 1265, 1330, 1836, 1894
BstPAI GACNNNNGTC 1 cut(s) 957
BstSCI CCNGG 9 cut(s) 13, 184, 554, 624, 787, 878, 890, 1087, 1268
BstSFI CTRYAG 1 cut(s) 798
BstV1I GCAGC 5 cut(s) 37, 737, 812, 837, 906
BstV2I GAAGAC 5 cut(s) 184, 211, 241, 1026, 1170
BstX2I RGATCY 4 cut(s) 384, 561, 1453, 1756
BstYI RGATCY 4 cut(s) 384, 561, 1453, 1756
BstZI CGGCCG 1 cut(s) 1271
Bsu36I CCTNAGG 1 cut(s) 1137
BsuRI GGCC 3 cut(s) 878, 965, 1273
BtgI CCRYGG 2 cut(s) 70, 1218
BtsCI GGATG 4 cut(s) 66, 1064, 1737, 1885
BtsIMutI CAGTG 2 cut(s) 117, 1851
Cac8I GCNNGC 7 cut(s) 848, 876, 1172, 1464, 1518, 1767, 1834
CfoI GCGC 1 cut(s) 874
Cfr10I RCCGGY 1 cut(s) 1462
Cfr13I GGNCC 4 cut(s) 4, 791, 959, 1804
CseI GACGC 3 cut(s) 178, 1181, 1552
Csp6I GTAC 2 cut(s) 1300, 1661
CviAII CATG 7 cut(s) 174, 1262, 1303, 1327, 1421, 1833, 1891
CviQI GTAC 2 cut(s) 1300, 1661
DdeI CTNAG 6 cut(s) 429, 593, 642, 1080, 1137, 1761
DpnI GATC 7 cut(s) 386, 563, 1211, 1455, 1667, 1758, 1899
DpnII GATC 7 cut(s) 384, 561, 1209, 1453, 1665, 1756, 1897
EaeI YGGCCR 3 cut(s) 876, 963, 1271
EagI CGGCCG 1 cut(s) 1271
Eam1104I CTCTTC 4 cut(s) 327, 442, 554, 815
EarI CTCTTC 4 cut(s) 327, 442, 554, 815
EciI GGCGGA 1 cut(s) 23
Ecl136II GAGCTC 2 cut(s) 316, 1042
EclXI CGGCCG 1 cut(s) 1271
Eco130I CCWWGG 1 cut(s) 683
Eco24I GRGCYC 2 cut(s) 318, 1044
Eco31I GGTCTC 1 cut(s) 1858
Eco47I GGWCC 4 cut(s) 4, 791, 959, 1804
Eco47III AGCGCT 1 cut(s) 873
Eco52I CGGCCG 1 cut(s) 1271
Eco53kI GAGCTC 2 cut(s) 316, 1042
Eco57I CTGAAG 4 cut(s) 81, 1365, 1386, 1478
Eco81I CCTNAGG 1 cut(s) 1137
Eco88I CYCGRG 2 cut(s) 854, 1655
EcoICRI GAGCTC 2 cut(s) 316, 1042
EcoRII CCWGG 3 cut(s) 624, 787, 1087
EcoT14I CCWWGG 1 cut(s) 683
EcoT22I ATGCAT 1 cut(s) 1182
EcoT38I GRGCYC 2 cut(s) 318, 1044
ErhI CCWWGG 1 cut(s) 683
FaeI CATG 7 cut(s) 177, 1265, 1306, 1330, 1424, 1836, 1894
FalI AAGNNNNNCTT 4 cut(s) 908, 940, 1011, 1043
FaqI GGGAC 4 cut(s) 241, 335, 1236, 1252
FatI CATG 7 cut(s) 173, 1261, 1302, 1326, 1420, 1832, 1890
FauI CCCGC 1 cut(s) 360
FbaI TGATCA 1 cut(s) 1665
Fnu4HI GCNGC 6 cut(s) 26, 726, 801, 851, 920, 1146
FokI GGATG 4 cut(s) 53, 1071, 1744, 1892
FriOI GRGCYC 2 cut(s) 318, 1044
Fsp4HI GCNGC 6 cut(s) 26, 726, 801, 851, 920, 1146
FspBI CTAG 1 cut(s) 240
GlaI GCGC 1 cut(s) 873
GluI GCNGC 6 cut(s) 26, 726, 801, 851, 920, 1146
GsaI CCCAGC 1 cut(s) 569
GsuI CTGGAG 3 cut(s) 401, 771, 1031
HaeII RGCGCY 1 cut(s) 875
HaeIII GGCC 3 cut(s) 878, 965, 1273
HgaI GACGC 3 cut(s) 178, 1181, 1552
HhaI GCGC 1 cut(s) 874
Hin1I GRCGYC 2 cut(s) 189, 1748
Hin1II CATG 7 cut(s) 177, 1265, 1306, 1330, 1424, 1836, 1894
Hin6I GCGC 1 cut(s) 872
HinP1I GCGC 1 cut(s) 872
HincII GTYRAC 3 cut(s) 343, 367, 1371
HindII GTYRAC 3 cut(s) 343, 367, 1371
HinfI GANTC 8 cut(s) 207, 243, 348, 953, 1240, 1395, 1717, 1775
HpaI GTTAAC 1 cut(s) 1371
HphI GGTGA 2 cut(s) 34, 428
Hpy166II GTNNAC 7 cut(s) 343, 367, 1064, 1072, 1371, 1426, 1617
Hpy188III TCNNGA 5 cut(s) 326, 502, 1010, 1552, 1699
Hpy8I GTNNAC 7 cut(s) 343, 367, 1064, 1072, 1371, 1426, 1617
Hpy99I CGWCG 1 cut(s) 1753
HpyAV CCTTC 6 cut(s) 55, 105, 113, 435, 623, 1340
HpyCH4III ACNGT 6 cut(s) 121, 1219, 1280, 1576, 1683, 1846
HpyCH4IV ACGT 3 cut(s) 225, 1470, 1748
HpyCH4V TGCA 9 cut(s) 177, 372, 800, 1180, 1388, 1505, 1589, 1842, 1890
HpyF10VI GCNNNNNNNGC 6 cut(s) 195, 304, 313, 495, 916, 1502
HpyF3I CTNAG 6 cut(s) 429, 593, 642, 1080, 1137, 1761
HpySE526I ACGT 3 cut(s) 225, 1470, 1748
Hsp92I GRCGYC 2 cut(s) 189, 1748
Hsp92II CATG 7 cut(s) 177, 1265, 1306, 1330, 1424, 1836, 1894
HspAI GCGC 1 cut(s) 872
Kpn2I TCCGGA 1 cut(s) 325
KroI GCCGGC 1 cut(s) 1462
KroNI GCCGGC 1 cut(s) 1464
Ksp22I TGATCA 1 cut(s) 1665
KspAI GTTAAC 1 cut(s) 1371
Kzo9I GATC 7 cut(s) 384, 561, 1209, 1453, 1665, 1756, 1897
LguI GCTCTTC 1 cut(s) 815
LmnI GCTCC 7 cut(s) 33, 638, 790, 869, 882, 1039, 1314
Lsp1109I GCAGC 5 cut(s) 37, 737, 812, 837, 906
LweI GCATC 2 cut(s) 1167, 1179
MaeI CTAG 1 cut(s) 240
MaeII ACGT 3 cut(s) 225, 1470, 1748
MaeIII GTNAC 1 cut(s) 1580
MalI GATC 7 cut(s) 386, 563, 1211, 1455, 1667, 1758, 1899
MboI GATC 7 cut(s) 384, 561, 1209, 1453, 1665, 1756, 1897
MflI RGATCY 4 cut(s) 384, 561, 1453, 1756
MhlI GDGCHC 2 cut(s) 318, 1044
MluCI AATT 6 cut(s) 745, 1197, 1227, 1500, 1691, 1781
MlyI GAGTC 6 cut(s) 201, 237, 357, 947, 1726, 1769
MmeI TCCRAC 6 cut(s) 237, 498, 1017, 1185, 1731, 1875
Mph1103I ATGCAT 1 cut(s) 1182
MroI TCCGGA 1 cut(s) 325
MroNI GCCGGC 1 cut(s) 1462
MroXI GAANNNNTTC 1 cut(s) 1399
MseI TTAA 5 cut(s) 309, 984, 1116, 1370, 1725
MspR9I CCNGG 9 cut(s) 15, 186, 556, 626, 789, 880, 892, 1089, 1270
MvaI CCWGG 3 cut(s) 626, 789, 1089
MwoI GCNNNNNNNGC 6 cut(s) 195, 304, 313, 495, 916, 1502
NaeI GCCGGC 1 cut(s) 1464
NciI CCSGG 6 cut(s) 15, 186, 556, 880, 892, 1270
NdeII GATC 7 cut(s) 384, 561, 1209, 1453, 1665, 1756, 1897
NgoMIV GCCGGC 1 cut(s) 1462
NlaIII CATG 7 cut(s) 177, 1265, 1306, 1330, 1424, 1836, 1894
NlaIV GGNNCC 4 cut(s) 6, 634, 681, 1758
NmeAIII GCCGAG 1 cut(s) 114
NsiI ATGCAT 1 cut(s) 1182
NspI RCATGY 4 cut(s) 1265, 1330, 1836, 1894
NspV TTCGAA 1 cut(s) 454
PaeI GCATGC 1 cut(s) 1836
PciI ACATGT 2 cut(s) 1261, 1326
PciSI GCTCTTC 1 cut(s) 815
PcsI WCGNNNNNNNCGW 1 cut(s) 1550
PdiI GCCGGC 1 cut(s) 1464
PdmI GAANNNNTTC 1 cut(s) 1399
PfeI GAWTC 2 cut(s) 1240, 1395
PflFI GACNNNGTC 1 cut(s) 347
PfoI TCCNGGA 2 cut(s) 554, 624
PkrI GCNGC 6 cut(s) 27, 727, 802, 852, 921, 1147
PleI GAGTC 6 cut(s) 201, 237, 356, 947, 1725, 1769
PpsI GAGTC 6 cut(s) 201, 237, 356, 947, 1725, 1769
PscI ACATGT 2 cut(s) 1261, 1326
PshAI GACNNNNGTC 1 cut(s) 957
Psp124BI GAGCTC 2 cut(s) 318, 1044
Psp6I CCWGG 3 cut(s) 624, 787, 1087
PspFI CCCAGC 1 cut(s) 565
PspGI CCWGG 3 cut(s) 624, 787, 1087
PspN4I GGNNCC 4 cut(s) 6, 634, 681, 1758
PspPI GGNCC 4 cut(s) 4, 791, 959, 1804
PstI CTGCAG 1 cut(s) 802
PsuI RGATCY 4 cut(s) 384, 561, 1453, 1756
PsyI GACNNNGTC 1 cut(s) 347
RsaI GTAC 2 cut(s) 1301, 1662
RsaNI GTAC 2 cut(s) 1300, 1661
SacI GAGCTC 2 cut(s) 318, 1044
SapI GCTCTTC 1 cut(s) 815
SaqAI TTAA 5 cut(s) 309, 984, 1116, 1370, 1725
SatI GCNGC 6 cut(s) 26, 726, 801, 851, 920, 1146
Sau3AI GATC 7 cut(s) 384, 561, 1209, 1453, 1665, 1756, 1897
Sau96I GGNCC 4 cut(s) 4, 791, 959, 1804
SchI GAGTC 6 cut(s) 201, 237, 357, 947, 1726, 1769
ScrFI CCNGG 9 cut(s) 15, 186, 556, 626, 789, 880, 892, 1089, 1270
SduI GDGCHC 2 cut(s) 318, 1044
SfaNI GCATC 2 cut(s) 1167, 1179
SfcI CTRYAG 1 cut(s) 798
SfuI TTCGAA 1 cut(s) 454
SinI GGWCC 4 cut(s) 4, 791, 959, 1804
SmlI CTYRAG 4 cut(s) 493, 756, 780, 1610
SmoI CTYRAG 4 cut(s) 493, 756, 780, 1610
SphI GCATGC 1 cut(s) 1836
Sse9I AATT 6 cut(s) 745, 1197, 1227, 1500, 1691, 1781
SsiI CCGC 7 cut(s) 23, 34, 75, 353, 636, 723, 1145
SspMI CTAG 1 cut(s) 240
SstI GAGCTC 2 cut(s) 318, 1044
StyD4I CCNGG 9 cut(s) 13, 184, 554, 624, 787, 878, 890, 1087, 1268
StyI CCWWGG 1 cut(s) 683
TaaI ACNGT 6 cut(s) 121, 1219, 1280, 1576, 1683, 1846
TaiI ACGT 3 cut(s) 228, 1473, 1751
TaqI TCGA 7 cut(s) 90, 454, 462, 657, 1231, 1498, 1773
TaqII GACCGA 2 cut(s) 360, 1191
TasI AATT 6 cut(s) 745, 1197, 1227, 1500, 1691, 1781
TatI WGTACW 1 cut(s) 1299
TauI GCSGC 1 cut(s) 1148
TfiI GAWTC 2 cut(s) 1240, 1395
Tru1I TTAA 5 cut(s) 309, 984, 1116, 1370, 1725
Tru9I TTAA 5 cut(s) 309, 984, 1116, 1370, 1725
TscAI CASTG 2 cut(s) 124, 1851
TseI GCWGC 5 cut(s) 25, 725, 800, 850, 919
TspDTI ATGAA 5 cut(s) 682, 886, 1319, 1704, 1748
TspGWI ACGGA 1 cut(s) 1172
TspRI CASTG 2 cut(s) 124, 1851
Tth111I GACNNNGTC 1 cut(s) 347
VpaK11BI GGWCC 4 cut(s) 4, 791, 959, 1804
XapI RAATTY 3 cut(s) 1197, 1227, 1691
XceI RCATGY 4 cut(s) 1265, 1330, 1836, 1894
XmnI GAANNNNTTC 1 cut(s) 1399
XspI CTAG 1 cut(s) 240
ZraI GACGTC 1 cut(s) 1749
Zsp2I ATGCAT 1 cut(s) 1182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.