Rorug07G0115900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0008718
Physical Location & Seq
Reverse (-)
9023898 .. 9025794
1897 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0115900.1

Sequence Viewer

Length: 1101 bp
ATGACTATGACCTCTACCGAGCCAAGCAGCAGAGATGGTTGCTTCATGGTCCGTGACAATGCCCTTCTGACACGTTTCCCGGCCAACATCACCGTCACTCCGGCCACTGGTGAATCAGCTTTCATCGGAGCCACCTCTGAAAACCGTAGCTCTCGCCATGTCTTCTCCCTCGGACTTCTTGAGGGATACAAGTTTATGTGCCTGTTCAGATTCAAAATCTGGTGGATGATACCGAGTTTTGGGGATTCTGGATGTGATGTTCCGGCAGAGACTCAAATGATTCTCTTGGAAGCAACGCAAACGTCGAGAAGTAGTAGTATTCCCGACAAAAATGGGTTCTCATCTGAGTCTGAGGAGCCAACAAGTGCTGAGAACAAAAGCACTTTCTACATTCTGCTATTGCCGGTCTTGGATGGAGCATTCAGGGCTACTTTGCAAGGCAACACTGTTAATGAGCTTGAGTTTTGCGTTGAAAGCGGGGACCGTGAAGTTCAAACCTCACGAGTAACTGAAGCAGTATTTGTGAATTCAGGAGATGACCCATATAAGCTCATTAGGGACTCTATTAAGATTCTTGCCAAGCACAAAGGTACATTCAATCATTTAGAAGACAAGAAGATACCAGCACATGTAGACTGGTTTGGATGGAACACATGGGATGCATTTTATAATGACGTTGATCCGGAAGGAATCGTGCTTGGACTCAAAAGTCTTTCCGAAGGAGGGTGCCCCCCGAAATGTTTGTACATCGATGAAGGATGGCAGACTAAAGTAATTGAAAATGAAACTGAACTACCAAATACAGCGAAGTTTGTGGCCAGGTTGGCAAGCTTAGAAGAAAATGATAGGTTCAAGGGCTACCCCTCGGGAAAGTCATCATTTCCTAATCTCCGCGAGTTTGTGAAAACCATCAAAGAGGAGCATGGACTCAAGTTAGTATATGCATGGCACGCTTTACTTGGTTCATGGGGAGGGCTACTGCCAACATCTGAAACACTGAGGAAATACAATCCCCAAATCAAATCCATAGTTCAGTCTCCTGGTAACAAGAGTAACGTCATATGTGGAACCCTCGATCCAATGGAGAAACATGGGATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

366

Amino Acids

40.76

Weight (kDa)

5.42

Isoelectric Point (pI)

42.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raffinose_syn PF05691 12 - 366 4.8e-98 Raffinose synthase or seed imbibition protein Sip1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 669
AasI GACNNNNNNGTC 1 cut(s) 708
AccB1I GGYRCC 1 cut(s) 726
AccI GTMKAC 1 cut(s) 633
AccII CGCG 1 cut(s) 894
AccIII TCCGGA 1 cut(s) 682
AciI CCGC 2 cut(s) 477, 892
AclWI GGATC 2 cut(s) 674, 1070
AcoI YGGCCR 3 cut(s) 81, 102, 816
AcsI RAATTY 1 cut(s) 526
AcuI CTGAAG 1 cut(s) 531
AfaI GTAC 2 cut(s) 592, 746
AfiI CCNNNNNNNGG 3 cut(s) 107, 239, 723
AflIII ACRYGT 2 cut(s) 71, 628
AgsI TTSAA 6 cut(s) 214, 473, 494, 598, 779, 853
AjnI CCWGG 2 cut(s) 818, 1039
AloI GAACNNNNNNTCC 4 cut(s) 243, 275, 1060, 1092
AluBI AGCT 5 cut(s) 119, 150, 457, 550, 831
AluI AGCT 5 cut(s) 119, 150, 457, 550, 831
Alw26I GTCTC 2 cut(s) 263, 1041
AlwI GGATC 2 cut(s) 674, 1070
Ama87I CYCGRG 1 cut(s) 865
Aor13HI TCCGGA 1 cut(s) 682
AoxI GGCC 3 cut(s) 81, 102, 816
ApeKI GCWGC 1 cut(s) 27
ApoI RAATTY 1 cut(s) 526
AspS9I GGNCC 2 cut(s) 49, 481
AsuC2I CCSGG 1 cut(s) 80
AsuHPI GGTGA 2 cut(s) 82, 122
AvaI CYCGRG 1 cut(s) 865
AvaII GGWCC 2 cut(s) 49, 481
BaeGI GKGCMC 1 cut(s) 731
BalI TGGCCA 1 cut(s) 818
BanI GGYRCC 1 cut(s) 726
BauI CACGAG 1 cut(s) 501
BbsI GAAGAC 2 cut(s) 154, 615
BbvI GCAGC 1 cut(s) 39
BccI CCATC 5 cut(s) 29, 407, 639, 753, 917
BciT130I CCWGG 2 cut(s) 820, 1041
BciVI GTATCC 1 cut(s) 179
BcnI CCSGG 1 cut(s) 80
BcoDI GTCTC 2 cut(s) 263, 1041
BfuI GTATCC 1 cut(s) 179
BglI GCCNNNNNGGC 1 cut(s) 824
BisI GCNGC 1 cut(s) 28
BlsI GCNGC 1 cut(s) 29
Bme1390I CCNGG 3 cut(s) 80, 820, 1041
Bme18I GGWCC 2 cut(s) 49, 481
BmeT110I CYCGRG 1 cut(s) 865
BmgT120I GGNCC 2 cut(s) 49, 481
BmiI GGNNCC 5 cut(s) 130, 357, 482, 728, 1069
BmrFI CCNGG 3 cut(s) 80, 820, 1041
BmsI GCATC 1 cut(s) 649
BpiI GAAGAC 2 cut(s) 154, 615
BpuEI CTTGAG 3 cut(s) 200, 479, 914
BpuMI CCSGG 1 cut(s) 80
Bsa29I ATCGAT 1 cut(s) 750
BsaJI CCNNGG 2 cut(s) 169, 864
BsaWI WCCGGW 1 cut(s) 682
BsaXI ACNNNNNCTCC 2 cut(s) 82, 112
Bsc4I CCNNNNNNNGG 3 cut(s) 107, 239, 723
Bse118I RCCGGY 1 cut(s) 403
Bse1I ACTGG 2 cut(s) 112, 641
BseAI TCCGGA 1 cut(s) 682
BseBI CCWGG 2 cut(s) 820, 1041
BseCI ATCGAT 1 cut(s) 750
BseDI CCNNGG 2 cut(s) 169, 864
BseGI GGATG 6 cut(s) 231, 257, 418, 650, 664, 764
BseLI CCNNNNNNNGG 3 cut(s) 107, 239, 723
BseMII CTCAG 4 cut(s) 336, 342, 360, 989
BseNI ACTGG 2 cut(s) 112, 641
BseRI GAGGAG 2 cut(s) 368, 932
BseSI GKGCMC 1 cut(s) 731
BseXI GCAGC 1 cut(s) 39
Bsh1236I CGCG 1 cut(s) 894
BshFI GGCC 3 cut(s) 83, 104, 818
BshNI GGYRCC 1 cut(s) 726
BshVI ATCGAT 1 cut(s) 750
BsiHKCI CYCGRG 1 cut(s) 865
BsiSI CCGG 5 cut(s) 80, 101, 263, 404, 683
BslFI GGGAC 2 cut(s) 494, 572
BslI CCNNNNNNNGG 3 cut(s) 107, 239, 723
BsmAI GTCTC 2 cut(s) 263, 1041
BsmFI GGGAC 2 cut(s) 494, 572
BsmI GAATGC 1 cut(s) 419
BsnI GGCC 3 cut(s) 83, 104, 818
BsoBI CYCGRG 1 cut(s) 865
Bsp1286I GDGCHC 1 cut(s) 731
Bsp13I TCCGGA 1 cut(s) 682
Bsp1407I TGTACA 1 cut(s) 744
Bsp143I GATC 3 cut(s) 679, 1075, 1095
BspACI CCGC 2 cut(s) 477, 892
BspANI GGCC 3 cut(s) 83, 104, 818
BspCNI CTCAG 4 cut(s) 337, 343, 361, 990
BspDI ATCGAT 1 cut(s) 750
BspEI TCCGGA 1 cut(s) 682
BspFNI CGCG 1 cut(s) 894
BspLI GGNNCC 5 cut(s) 130, 357, 482, 728, 1069
BspPI GGATC 2 cut(s) 674, 1070
BspT107I GGYRCC 1 cut(s) 726
BsrFI RCCGGY 1 cut(s) 403
BsrGI TGTACA 1 cut(s) 744
BsrI ACTGG 2 cut(s) 112, 641
BssAI RCCGGY 1 cut(s) 403
BssECI CCNNGG 2 cut(s) 169, 864
BssMI GATC 3 cut(s) 679, 1075, 1095
BssSI CACGAG 1 cut(s) 501
Bst2BI CACGAG 1 cut(s) 501
Bst2UI CCWGG 2 cut(s) 820, 1041
Bst4CI ACNGT 4 cut(s) 94, 146, 448, 485
BstAUI TGTACA 1 cut(s) 744
BstC8I GCNNGC 2 cut(s) 829, 951
BstDEI CTNAG 5 cut(s) 345, 351, 369, 832, 998
BstF5I GGATG 6 cut(s) 231, 257, 418, 650, 664, 764
BstFNI CGCG 1 cut(s) 894
BstKTI GATC 3 cut(s) 682, 1078, 1098
BstMAI GTCTC 2 cut(s) 263, 1041
BstMBI GATC 3 cut(s) 679, 1075, 1095
BstMWI GCNNNNNNNGC 4 cut(s) 425, 474, 824, 950
BstNI CCWGG 2 cut(s) 820, 1041
BstNSI RCATGY 1 cut(s) 632
BstSCI CCNGG 3 cut(s) 78, 818, 1039
BstSLI GKGCMC 1 cut(s) 731
BstUI CGCG 1 cut(s) 894
BstV1I GCAGC 1 cut(s) 39
BstV2I GAAGAC 2 cut(s) 154, 615
BstX2I RGATCY 1 cut(s) 1095
BstYI RGATCY 1 cut(s) 1095
Bsu15I ATCGAT 1 cut(s) 750
BsuI GTATCC 1 cut(s) 179
BsuRI GGCC 3 cut(s) 83, 104, 818
BsuTUI ATCGAT 1 cut(s) 750
BtsCI GGATG 6 cut(s) 231, 257, 418, 650, 664, 764
BtsIMutI CAGTG 3 cut(s) 105, 444, 995
Cac8I GCNNGC 2 cut(s) 829, 951
Cfr10I RCCGGY 1 cut(s) 403
Cfr13I GGNCC 2 cut(s) 49, 481
ClaI ATCGAT 1 cut(s) 750
Csp6I GTAC 2 cut(s) 591, 745
CviAII CATG 8 cut(s) 46, 158, 629, 654, 923, 945, 966, 1091
CviQI GTAC 2 cut(s) 591, 745
DdeI CTNAG 5 cut(s) 345, 351, 369, 832, 998
DpnI GATC 3 cut(s) 681, 1077, 1097
DpnII GATC 3 cut(s) 679, 1075, 1095
DrdI GACNNNNNNGTC 1 cut(s) 708
DseDI GACNNNNNNGTC 1 cut(s) 708
EaeI YGGCCR 3 cut(s) 81, 102, 816
Eco47I GGWCC 2 cut(s) 49, 481
Eco57I CTGAAG 1 cut(s) 531
Eco88I CYCGRG 1 cut(s) 865
EcoRI GAATTC 1 cut(s) 526
EcoRII CCWGG 2 cut(s) 818, 1039
EcoT22I ATGCAT 2 cut(s) 664, 946
FaeI CATG 8 cut(s) 49, 161, 632, 657, 926, 948, 969, 1094
FaqI GGGAC 2 cut(s) 494, 572
FatI CATG 8 cut(s) 45, 157, 628, 653, 922, 944, 965, 1090
FauI CCCGC 1 cut(s) 470
FauNDI CATATG 1 cut(s) 1061
FblI GTMKAC 1 cut(s) 633
Fnu4HI GCNGC 1 cut(s) 28
FokI GGATG 6 cut(s) 238, 264, 425, 657, 671, 771
Fsp4HI GCNGC 1 cut(s) 28
GluI GCNGC 1 cut(s) 28
HaeIII GGCC 3 cut(s) 83, 104, 818
HapII CCGG 5 cut(s) 80, 101, 263, 404, 683
Hin1II CATG 8 cut(s) 49, 161, 632, 657, 926, 948, 969, 1094
HindIII AAGCTT 1 cut(s) 829
HpaII CCGG 5 cut(s) 80, 101, 263, 404, 683
HphI GGTGA 2 cut(s) 82, 122
Hpy166II GTNNAC 1 cut(s) 634
Hpy188III TCNNGA 8 cut(s) 179, 249, 306, 323, 501, 531, 683, 867
Hpy8I GTNNAC 1 cut(s) 634
Hpy99I CGWCG 1 cut(s) 307
HpyAV CCTTC 4 cut(s) 74, 680, 713, 749
HpyCH4III ACNGT 4 cut(s) 94, 146, 448, 485
HpyCH4IV ACGT 4 cut(s) 73, 302, 675, 1056
HpyCH4V TGCA 3 cut(s) 436, 662, 944
HpyF10VI GCNNNNNNNGC 4 cut(s) 425, 474, 824, 950
HpyF3I CTNAG 5 cut(s) 345, 351, 369, 832, 998
HpySE526I ACGT 4 cut(s) 73, 302, 675, 1056
Hsp92II CATG 8 cut(s) 49, 161, 632, 657, 926, 948, 969, 1094
Kpn2I TCCGGA 1 cut(s) 682
Kzo9I GATC 3 cut(s) 679, 1075, 1095
LmnI GCTCC 4 cut(s) 128, 355, 416, 919
Lsp1109I GCAGC 1 cut(s) 39
LweI GCATC 1 cut(s) 649
MaeII ACGT 4 cut(s) 73, 302, 675, 1056
MaeIII GTNAC 5 cut(s) 53, 94, 505, 1043, 1052
MalI GATC 3 cut(s) 681, 1077, 1097
MboI GATC 3 cut(s) 679, 1075, 1095
MboII GAAGA 4 cut(s) 154, 620, 628, 848
MflI RGATCY 1 cut(s) 1095
MhlI GDGCHC 1 cut(s) 731
MlsI TGGCCA 1 cut(s) 818
MluCI AATT 2 cut(s) 526, 774
MluNI TGGCCA 1 cut(s) 818
MlyI GAGTC 5 cut(s) 265, 356, 554, 696, 921
Mox20I TGGCCA 1 cut(s) 818
Mph1103I ATGCAT 2 cut(s) 664, 946
MroI TCCGGA 1 cut(s) 682
MscI TGGCCA 1 cut(s) 818
MseI TTAA 2 cut(s) 450, 567
Msp20I TGGCCA 1 cut(s) 818
MspI CCGG 5 cut(s) 80, 101, 263, 404, 683
MspR9I CCNGG 3 cut(s) 80, 820, 1041
Mva1269I GAATGC 1 cut(s) 419
MvaI CCWGG 2 cut(s) 820, 1041
MvnI CGCG 1 cut(s) 894
MwoI GCNNNNNNNGC 4 cut(s) 425, 474, 824, 950
NciI CCSGG 1 cut(s) 80
NdeI CATATG 1 cut(s) 1061
NdeII GATC 3 cut(s) 679, 1075, 1095
NlaIII CATG 8 cut(s) 49, 161, 632, 657, 926, 948, 969, 1094
NlaIV GGNNCC 5 cut(s) 130, 357, 482, 728, 1069
NmuCI GTSAC 2 cut(s) 53, 94
NsiI ATGCAT 2 cut(s) 664, 946
NspI RCATGY 1 cut(s) 632
PciI ACATGT 1 cut(s) 628
PcsI WCGNNNNNNNCGW 1 cut(s) 302
PctI GAATGC 1 cut(s) 419
PfeI GAWTC 6 cut(s) 113, 210, 245, 280, 571, 690
PkrI GCNGC 1 cut(s) 29
PleI GAGTC 5 cut(s) 265, 355, 554, 696, 921
PpsI GAGTC 5 cut(s) 265, 355, 554, 696, 921
PscI ACATGT 1 cut(s) 628
PsiI TTATAA 1 cut(s) 669
Psp6I CCWGG 2 cut(s) 818, 1039
PspGI CCWGG 2 cut(s) 818, 1039
PspN4I GGNNCC 5 cut(s) 130, 357, 482, 728, 1069
PspPI GGNCC 2 cut(s) 49, 481
PsuI RGATCY 1 cut(s) 1095
RsaI GTAC 2 cut(s) 592, 746
RsaNI GTAC 2 cut(s) 591, 745
SaqAI TTAA 2 cut(s) 450, 567
SatI GCNGC 1 cut(s) 28
Sau3AI GATC 3 cut(s) 679, 1075, 1095
Sau96I GGNCC 2 cut(s) 49, 481
SchI GAGTC 5 cut(s) 265, 356, 554, 696, 921
ScrFI CCNGG 3 cut(s) 80, 820, 1041
SduI GDGCHC 1 cut(s) 731
SfaNI GCATC 1 cut(s) 649
SinI GGWCC 2 cut(s) 49, 481
SmlI CTYRAG 3 cut(s) 179, 458, 929
SmoI CTYRAG 3 cut(s) 179, 458, 929
Sse9I AATT 2 cut(s) 526, 774
SsiI CCGC 2 cut(s) 477, 892
StyD4I CCNGG 3 cut(s) 78, 818, 1039
TaaI ACNGT 4 cut(s) 94, 146, 448, 485
TaiI ACGT 4 cut(s) 76, 305, 678, 1059
TaqI TCGA 3 cut(s) 305, 750, 1074
TasI AATT 2 cut(s) 526, 774
TatI WGTACW 1 cut(s) 744
TfiI GAWTC 6 cut(s) 113, 210, 245, 280, 571, 690
Tru1I TTAA 2 cut(s) 450, 567
Tru9I TTAA 2 cut(s) 450, 567
TscAI CASTG 3 cut(s) 112, 451, 1002
TseFI GTSAC 2 cut(s) 53, 94
TseI GCWGC 1 cut(s) 27
Tsp45I GTSAC 2 cut(s) 53, 94
TspDTI ATGAA 5 cut(s) 34, 112, 768, 798, 954
TspGWI ACGGA 1 cut(s) 41
TspRI CASTG 3 cut(s) 112, 451, 1002
VpaK11BI GGWCC 2 cut(s) 49, 481
XapI RAATTY 1 cut(s) 526
XceI RCATGY 1 cut(s) 632
XmiI GTMKAC 1 cut(s) 633
Zsp2I ATGCAT 2 cut(s) 664, 946
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.