Rh1AG217700

SPX domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0007102
Physical Location & Seq
Forward (+)
41452447 .. 41455432
2986 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG217700.1

Sequence Viewer

Length: 546 bp
ATGAAGGAATTGCAGCAGAGAATAAAAAAAGTAACTGACACATGGGGACCGAATGGTAGTCATGCTTCAAAGACTACATATGAAGATGAAGTCGGGAAGCTTAGAAAAGACATTGTTGATTTCCACGGTGAAATGGTGCTCTTGGTCAACTACAGCAATATCAACTACACAGGATTGGCCAAAATACTGAAGAAGTATGACAAGAGAACAGGTGCTCTGCTGCGCTTGCCATTCATCCAAAAAATACTAGAGCAGCCCTTCTTCACCACTGATCTTATCTCAAAGCTTGTCAAGGAATGTGAAGGCAGCATCGATGCAGTGTTCCCAGTGGAGAAAGAAGAGGAGAGGAAAAAAGAAGTAAGGGAAGCAATAACAGTTGGCGGGGAAGGAATATTTCGAAACACAGTTGTAGCTCTACTGACAATGCAAGAAATCAGAAGAGGAAGCTCTACTTACAGTCAGTATTCTCTACCACCAATCAACATGCCTGCAGACTCGGATCTCATCCAGTTAGTCCAACTCAACCCTCCCATAGCAATAATCTAG

Protein Analysis

181

Amino Acids

20.51

Weight (kDa)

7.77

Isoelectric Point (pI)

36.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPX PF03105 6 - 81 2.8e-11 SPX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 381
AclWI GGATC 1 cut(s) 507
AcoI YGGCCR 1 cut(s) 177
AcuI CTGAAG 1 cut(s) 209
AgsI TTSAA 1 cut(s) 69
AluBI AGCT 4 cut(s) 100, 286, 413, 447
AluI AGCT 4 cut(s) 100, 286, 413, 447
Alw21I GWGCWC 2 cut(s) 141, 217
AlwI GGATC 1 cut(s) 507
AoxI GGCC 1 cut(s) 177
ApeKI GCWGC 4 cut(s) 13, 220, 253, 306
AspLEI GCGC 1 cut(s) 225
AspS9I GGNCC 1 cut(s) 47
AsuHPI GGTGA 2 cut(s) 140, 256
AsuII TTCGAA 1 cut(s) 397
AvaII GGWCC 1 cut(s) 47
BalI TGGCCA 1 cut(s) 179
BarI GAAGNNNNNNTAC 2 cut(s) 49, 81
Bbv12I GWGCWC 2 cut(s) 141, 217
BbvI GCAGC 4 cut(s) 25, 207, 265, 318
BfaI CTAG 2 cut(s) 248, 544
BfmI CTRYAG 2 cut(s) 151, 489
BisI GCNGC 4 cut(s) 14, 221, 254, 307
BlsI GCNGC 4 cut(s) 15, 222, 255, 308
Bme18I GGWCC 1 cut(s) 47
BmgT120I GGNCC 1 cut(s) 47
BmiI GGNNCC 1 cut(s) 48
BmrI ACTGGG 1 cut(s) 320
BmsI GCATC 2 cut(s) 304, 318
BmuI ACTGGG 1 cut(s) 320
Bpu14I TTCGAA 1 cut(s) 397
Bsa29I ATCGAT 1 cut(s) 312
BsaJI CCNNGG 1 cut(s) 124
Bse1I ACTGG 2 cut(s) 326, 508
BseCI ATCGAT 1 cut(s) 312
BseDI CCNNGG 1 cut(s) 124
BseGI GGATG 2 cut(s) 234, 504
BseNI ACTGG 2 cut(s) 326, 508
BseRI GAGGAG 1 cut(s) 356
BseXI GCAGC 4 cut(s) 25, 207, 265, 318
BshFI GGCC 1 cut(s) 179
BshVI ATCGAT 1 cut(s) 312
BsiHKAI GWGCWC 2 cut(s) 141, 217
BslFI GGGAC 1 cut(s) 60
BsmFI GGGAC 1 cut(s) 60
BsnI GGCC 1 cut(s) 179
Bsp119I TTCGAA 1 cut(s) 397
Bsp1286I GDGCHC 2 cut(s) 141, 217
Bsp143I GATC 2 cut(s) 271, 499
BspACI CCGC 1 cut(s) 381
BspANI GGCC 1 cut(s) 179
BspDI ATCGAT 1 cut(s) 312
BspLI GGNNCC 1 cut(s) 48
BspMAI CTGCAG 1 cut(s) 493
BspPI GGATC 1 cut(s) 507
BspT104I TTCGAA 1 cut(s) 397
BsrI ACTGG 2 cut(s) 326, 508
BssECI CCNNGG 1 cut(s) 124
BssMI GATC 2 cut(s) 271, 499
Bst4CI ACNGT 4 cut(s) 128, 376, 406, 458
Bst6I CTCTTC 2 cut(s) 333, 433
BstBI TTCGAA 1 cut(s) 397
BstC8I GCNNGC 2 cut(s) 227, 489
BstDEI CTNAG 1 cut(s) 101
BstDSI CCRYGG 1 cut(s) 124
BstF5I GGATG 2 cut(s) 234, 504
BstHHI GCGC 1 cut(s) 225
BstKTI GATC 2 cut(s) 274, 502
BstMBI GATC 2 cut(s) 271, 499
BstMWI GCNNNNNNNGC 1 cut(s) 226
BstNSI RCATGY 1 cut(s) 487
BstSFI CTRYAG 2 cut(s) 151, 489
BstV1I GCAGC 4 cut(s) 25, 207, 265, 318
BstX2I RGATCY 1 cut(s) 499
BstYI RGATCY 1 cut(s) 499
Bsu15I ATCGAT 1 cut(s) 312
BsuRI GGCC 1 cut(s) 179
BsuTUI ATCGAT 1 cut(s) 312
BtgI CCRYGG 1 cut(s) 124
BtsCI GGATG 2 cut(s) 234, 504
BtsI GCAGTG 1 cut(s) 324
BtsIMutI CAGTG 3 cut(s) 267, 324, 333
Cac8I GCNNGC 2 cut(s) 227, 489
CfoI GCGC 1 cut(s) 225
Cfr13I GGNCC 1 cut(s) 47
ClaI ATCGAT 1 cut(s) 312
CviAII CATG 3 cut(s) 42, 62, 484
CviJI RGCY 6 cut(s) 100, 179, 256, 286, 413, 447
CviKI_1 RGCY 6 cut(s) 100, 179, 256, 286, 413, 447
DdeI CTNAG 1 cut(s) 101
DpnI GATC 2 cut(s) 273, 501
DpnII GATC 2 cut(s) 271, 499
EaeI YGGCCR 1 cut(s) 177
Eam1104I CTCTTC 2 cut(s) 333, 433
EarI CTCTTC 2 cut(s) 333, 433
Eco47I GGWCC 1 cut(s) 47
Eco57I CTGAAG 1 cut(s) 209
FaeI CATG 3 cut(s) 45, 65, 487
FaiI YATR 7 cut(s) 43, 63, 79, 81, 198, 485, 533
FalI AAGNNNNNCTT 2 cut(s) 436, 468
FaqI GGGAC 1 cut(s) 60
FatI CATG 3 cut(s) 41, 61, 483
FauI CCCGC 1 cut(s) 374
FauNDI CATATG 1 cut(s) 79
Fnu4HI GCNGC 4 cut(s) 14, 221, 254, 307
FokI GGATG 2 cut(s) 221, 491
Fsp4HI GCNGC 4 cut(s) 14, 221, 254, 307
FspBI CTAG 2 cut(s) 248, 544
GlaI GCGC 1 cut(s) 224
GluI GCNGC 4 cut(s) 14, 221, 254, 307
HaeIII GGCC 1 cut(s) 179
HhaI GCGC 1 cut(s) 225
Hin1II CATG 3 cut(s) 45, 65, 487
Hin6I GCGC 1 cut(s) 223
HinP1I GCGC 1 cut(s) 223
HincII GTYRAC 1 cut(s) 148
HindII GTYRAC 1 cut(s) 148
HindIII AAGCTT 2 cut(s) 98, 284
HinfI GANTC 1 cut(s) 494
HphI GGTGA 2 cut(s) 140, 256
Hpy166II GTNNAC 1 cut(s) 148
Hpy188I TCNGA 2 cut(s) 437, 499
Hpy188III TCNNGA 1 cut(s) 94
Hpy8I GTNNAC 1 cut(s) 148
HpyAV CCTTC 3 cut(s) 268, 296, 380
HpyCH4III ACNGT 4 cut(s) 128, 376, 406, 458
HpyCH4V TGCA 4 cut(s) 13, 317, 427, 491
HpyF10VI GCNNNNNNNGC 1 cut(s) 226
HpyF3I CTNAG 1 cut(s) 101
Hsp92II CATG 3 cut(s) 45, 65, 487
HspAI GCGC 1 cut(s) 223
Kzo9I GATC 2 cut(s) 271, 499
LpnPI CCDG 5 cut(s) 156, 195, 339, 501, 521
Lsp1109I GCAGC 4 cut(s) 25, 207, 265, 318
LweI GCATC 2 cut(s) 304, 318
MaeI CTAG 2 cut(s) 248, 544
MaeIII GTNAC 1 cut(s) 31
MalI GATC 2 cut(s) 273, 501
MboI GATC 2 cut(s) 271, 499
MboII GAAGA 5 cut(s) 95, 202, 253, 350, 450
MflI RGATCY 1 cut(s) 499
MhlI GDGCHC 2 cut(s) 141, 217
MlsI TGGCCA 1 cut(s) 179
MluCI AATT 1 cut(s) 8
MluNI TGGCCA 1 cut(s) 179
MlyI GAGTC 1 cut(s) 488
MmeI TCCRAC 1 cut(s) 541
MnlI CCTC 4 cut(s) 334, 339, 434, 537
Mox20I TGGCCA 1 cut(s) 179
MscI TGGCCA 1 cut(s) 179
Msp20I TGGCCA 1 cut(s) 179
MwoI GCNNNNNNNGC 1 cut(s) 226
NdeI CATATG 1 cut(s) 79
NdeII GATC 2 cut(s) 271, 499
NlaIII CATG 3 cut(s) 45, 65, 487
NlaIV GGNNCC 1 cut(s) 48
NspI RCATGY 1 cut(s) 487
NspV TTCGAA 1 cut(s) 397
PkrI GCNGC 4 cut(s) 15, 222, 255, 308
PleI GAGTC 1 cut(s) 488
PpsI GAGTC 1 cut(s) 488
PspN4I GGNNCC 1 cut(s) 48
PspPI GGNCC 1 cut(s) 47
PstI CTGCAG 1 cut(s) 493
PsuI RGATCY 1 cut(s) 499
SatI GCNGC 4 cut(s) 14, 221, 254, 307
Sau3AI GATC 2 cut(s) 271, 499
Sau96I GGNCC 1 cut(s) 47
SchI GAGTC 1 cut(s) 488
SduI GDGCHC 2 cut(s) 141, 217
SetI ASST 5 cut(s) 102, 214, 288, 415, 449
SfaNI GCATC 2 cut(s) 304, 318
SfcI CTRYAG 2 cut(s) 151, 489
SfuI TTCGAA 1 cut(s) 397
SinI GGWCC 1 cut(s) 47
Sse9I AATT 1 cut(s) 8
SsiI CCGC 1 cut(s) 381
SspI AATATT 1 cut(s) 393
SspMI CTAG 2 cut(s) 248, 544
TaaI ACNGT 4 cut(s) 128, 376, 406, 458
TaqI TCGA 2 cut(s) 312, 397
TaqII GACCGA 1 cut(s) 64
TasI AATT 1 cut(s) 8
TscAI CASTG 3 cut(s) 274, 324, 333
TseI GCWGC 4 cut(s) 13, 220, 253, 306
TspDTI ATGAA 4 cut(s) 17, 96, 102, 223
TspRI CASTG 3 cut(s) 274, 324, 333
VpaK11BI GGWCC 1 cut(s) 47
XceI RCATGY 1 cut(s) 487
XspI CTAG 2 cut(s) 248, 544
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.