Rh1DG077000

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0008503
Physical Location & Seq
Reverse (-)
14200870 .. 14224348
23479 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG077000.1

Sequence Viewer

Length: 933 bp
ATGGCGCCTCTTCGATGGTTTCTCCTTCTCAGCCTCATTTGCAGTATTCTACTGCTTGTCAATGTTACTCACTCAACTGCTCAGGATACCCGAAAGGCTTATGTTGTGTATATGGGCGACAAGCCAAAGAACGGGGTACCCATATTACCTGATCTTCATGTGAATATGCTACAGGACGTAGTTGACAGCAGCAACATTAATATTGCACATGAAGCTCTGCTTCTTCACAGCTACAAGAGAAGTTTCAATGGATTTGCTGCAATGCTCACAGAGCAAGAAGCACAAAAAATGACCGCTAAGCCTACCGAGATTGGCTATGAGAGAGGAAGTGAAGCTCATGAGCACGAGAAAGAAGGAGATGAGCTCATGAGTGCAGCTGGGTCTCTGGTGCGTACACCATGGCTGAATCGTTCGACTGAACTCAAGGGTGGAGCAAACATGTCGTTCACACTCCGGAGCGTGAAGGTGCCACCGAACTCGGTCTCTCTCGACGAAGCCCGGCACCGAGTCTTCGACTTCTTCAGGGCCGCCTGCAGATCCATCCCTACAGTCATGGACATCTACAACCTTCACGACGTCGTCGCCCCATCTCACCTCCGCTCCGTCGTCGCCGCCGAGATCCGCAAGCACGCCTCCGTCACCAACCCCAAGATGCATTGTTCCTGCTTTGCATCAAAAAGCTTCATTGTCAAAGAATTTAAGCTTGGATCTTTGCGATCGCAACCTCTTGGTTTTTGTGACATTTCGTTTGTTGGGTTCTATGGTGGTAGTCGAGGTAAGAGGATTGATACTCTGGAGCTGAGCACTATGGAGGATGTTGGTACTTCTTCTCCTCCAAAGAGCTCAAAGAGATGGATTACGAGGATGAAAACCAGAGGTGAAAAGGTGGGTGATAGAGATGTAGGTCATCAATTGAGATTTCTGGCAGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

310

Amino Acids

34.51

Weight (kDa)

9.25

Isoelectric Point (pI)

53.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 34 - 101 1.4e-09 Peptidase inhibitor I9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 579
Acc36I ACCTGC 1 cut(s) 917
Acc65I GGTACC 1 cut(s) 136
AccB1I GGYRCC 4 cut(s) 4, 136, 466, 501
AccBSI CCGCTC 1 cut(s) 600
AccIII TCCGGA 1 cut(s) 453
AciI CCGC 5 cut(s) 294, 528, 598, 612, 622
AclWI GGATC 3 cut(s) 531, 613, 715
AcsI RAATTY 1 cut(s) 695
AcuI CTGAAG 1 cut(s) 505
AcyI GRCGYC 2 cut(s) 5, 576
AfaI GTAC 3 cut(s) 138, 394, 823
AfiI CCNNNNNNNGG 1 cut(s) 131
AflIII ACRYGT 1 cut(s) 438
AgsI TTSAA 1 cut(s) 247
AjuI GAANNNNNNNTTGG 2 cut(s) 687, 719
AluBI AGCT 9 cut(s) 215, 231, 335, 364, 377, 681, 703, 799, 843
AluI AGCT 9 cut(s) 215, 231, 335, 364, 377, 681, 703, 799, 843
Alw21I GWGCWC 4 cut(s) 345, 366, 806, 845
Alw26I GTCTC 2 cut(s) 387, 487
AlwI GGATC 3 cut(s) 531, 613, 715
Aor13HI TCCGGA 1 cut(s) 453
AoxI GGCC 1 cut(s) 525
ApeKI GCWGC 3 cut(s) 189, 257, 374
ApoI RAATTY 1 cut(s) 695
ArsI GACNNNNNNTTYG 2 cut(s) 731, 763
AseI ATTAAT 1 cut(s) 198
AsiSI GCGATCGC 1 cut(s) 719
Asp718I GGTACC 1 cut(s) 136
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 1 cut(s) 525
AsuC2I CCSGG 1 cut(s) 499
AsuHPI GGTGA 4 cut(s) 584, 631, 890, 902
BanI GGYRCC 4 cut(s) 4, 136, 466, 501
BanII GRGCYC 2 cut(s) 366, 845
BauI CACGAG 1 cut(s) 344
BbsI GAAGAC 1 cut(s) 502
Bbv12I GWGCWC 4 cut(s) 345, 366, 806, 845
BbvI GCAGC 3 cut(s) 201, 244, 386
BccI CCATC 4 cut(s) 9, 548, 595, 846
BcgI CGANNNNNNTGC 2 cut(s) 423, 457
BciVI GTATCC 1 cut(s) 79
BcnI CCSGG 1 cut(s) 499
BcoDI GTCTC 2 cut(s) 387, 487
BfmI CTRYAG 3 cut(s) 170, 532, 546
BfoI RGCGCY 1 cut(s) 8
BfuAI ACCTGC 1 cut(s) 917
BfuI GTATCC 1 cut(s) 79
BisI GCNGC 5 cut(s) 190, 258, 375, 528, 612
BlpI GCTNAGC 2 cut(s) 297, 800
BlsI GCNGC 5 cut(s) 191, 259, 376, 529, 613
Bme1390I CCNGG 1 cut(s) 499
BmgT120I GGNCC 1 cut(s) 525
BmiI GGNNCC 4 cut(s) 6, 138, 468, 503
BmrFI CCNGG 1 cut(s) 499
BmsI GCATC 2 cut(s) 642, 680
BpiI GAAGAC 1 cut(s) 502
BplI GAGNNNNNCTC 2 cut(s) 348, 380
BpmI CTGGAG 1 cut(s) 815
Bpu10I CCTNAGC 1 cut(s) 81
Bpu1102I GCTNAGC 2 cut(s) 297, 800
BpuEI CTTGAG 1 cut(s) 407
BpuMI CCSGG 1 cut(s) 499
BsaHI GRCGYC 2 cut(s) 5, 576
BsaI GGTCTC 2 cut(s) 387, 487
BsaJI CCNNGG 1 cut(s) 398
BsaWI WCCGGW 1 cut(s) 453
BsaXI ACNNNNNCTCC 4 cut(s) 584, 614, 814, 844
Bsc4I CCNNNNNNNGG 1 cut(s) 131
Bse3DI GCAATG 1 cut(s) 267
BseAI TCCGGA 1 cut(s) 453
BseDI CCNNGG 1 cut(s) 398
BseGI GGATG 3 cut(s) 540, 820, 870
BseLI CCNNNNNNNGG 1 cut(s) 131
BseMI GCAATG 1 cut(s) 267
BseMII CTCAG 3 cut(s) 43, 95, 791
BseRI GAGGAG 1 cut(s) 822
BseXI GCAGC 3 cut(s) 201, 244, 386
BseYI CCCAGC 1 cut(s) 377
BsgI GTGCAG 1 cut(s) 393
Bsh1285I CGRYCG 1 cut(s) 719
BshFI GGCC 1 cut(s) 527
BshNI GGYRCC 4 cut(s) 4, 136, 466, 501
BsiEI CGRYCG 1 cut(s) 719
BsiHKAI GWGCWC 4 cut(s) 345, 366, 806, 845
BsiSI CCGG 2 cut(s) 454, 499
BslI CCNNNNNNNGG 1 cut(s) 131
BsmAI GTCTC 2 cut(s) 387, 487
BsnI GGCC 1 cut(s) 527
Bso31I GGTCTC 2 cut(s) 387, 487
Bsp1286I GDGCHC 4 cut(s) 345, 366, 806, 845
Bsp13I TCCGGA 1 cut(s) 453
Bsp143I GATC 5 cut(s) 151, 536, 618, 707, 716
Bsp1720I GCTNAGC 2 cut(s) 297, 800
Bsp19I CCATGG 1 cut(s) 398
BspACI CCGC 5 cut(s) 294, 528, 598, 612, 622
BspANI GGCC 1 cut(s) 527
BspCNI CTCAG 3 cut(s) 42, 94, 792
BspEI TCCGGA 1 cut(s) 453
BspHI TCATGA 2 cut(s) 337, 366
BspLI GGNNCC 4 cut(s) 6, 138, 468, 503
BspMAI CTGCAG 1 cut(s) 536
BspMI ACCTGC 1 cut(s) 917
BspPI GGATC 3 cut(s) 531, 613, 715
BspT107I GGYRCC 4 cut(s) 4, 136, 466, 501
BspTNI GGTCTC 2 cut(s) 387, 487
BsrBI CCGCTC 1 cut(s) 600
BsrDI GCAATG 1 cut(s) 267
BssECI CCNNGG 1 cut(s) 398
BssMI GATC 5 cut(s) 151, 536, 618, 707, 716
BssNI GRCGYC 2 cut(s) 5, 576
BssSI CACGAG 1 cut(s) 344
BssT1I CCWWGG 1 cut(s) 398
Bst2BI CACGAG 1 cut(s) 344
Bst4CI ACNGT 1 cut(s) 550
Bst6I CTCTTC 1 cut(s) 15
BstACI GRCGYC 2 cut(s) 5, 576
BstC8I GCNNGC 3 cut(s) 532, 626, 630
BstDEI CTNAG 4 cut(s) 29, 81, 297, 800
BstDSI CCRYGG 1 cut(s) 398
BstF5I GGATG 3 cut(s) 540, 820, 870
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 5 cut(s) 154, 539, 621, 710, 719
BstMAI GTCTC 2 cut(s) 387, 487
BstMBI GATC 5 cut(s) 151, 536, 618, 707, 716
BstMCI CGRYCG 1 cut(s) 719
BstMWI GCNNNNNNNGC 3 cut(s) 39, 212, 271
BstNSI RCATGY 1 cut(s) 442
BstSCI CCNGG 1 cut(s) 497
BstSFI CTRYAG 3 cut(s) 170, 532, 546
BstV1I GCAGC 3 cut(s) 201, 244, 386
BstV2I GAAGAC 1 cut(s) 502
BstX2I RGATCY 3 cut(s) 536, 618, 707
BstYI RGATCY 3 cut(s) 536, 618, 707
BsuI GTATCC 1 cut(s) 79
BsuRI GGCC 1 cut(s) 527
BtgI CCRYGG 1 cut(s) 398
BtsCI GGATG 3 cut(s) 540, 820, 870
BveI ACCTGC 1 cut(s) 917
Cac8I GCNNGC 3 cut(s) 532, 626, 630
CciI TCATGA 2 cut(s) 337, 366
CfoI GCGC 1 cut(s) 7
Cfr13I GGNCC 1 cut(s) 525
Csp6I GTAC 3 cut(s) 137, 393, 822
CviAII CATG 7 cut(s) 158, 209, 338, 367, 399, 439, 553
CviQI GTAC 3 cut(s) 137, 393, 822
DdeI CTNAG 4 cut(s) 29, 81, 297, 800
DinI GGCGCC 1 cut(s) 6
DpnI GATC 5 cut(s) 153, 538, 620, 709, 718
DpnII GATC 5 cut(s) 151, 536, 618, 707, 716
Eam1104I CTCTTC 1 cut(s) 15
EarI CTCTTC 1 cut(s) 15
Ecl136II GAGCTC 2 cut(s) 364, 843
Eco130I CCWWGG 1 cut(s) 398
Eco24I GRGCYC 2 cut(s) 366, 845
Eco31I GGTCTC 2 cut(s) 387, 487
Eco53kI GAGCTC 2 cut(s) 364, 843
Eco57I CTGAAG 1 cut(s) 505
EcoICRI GAGCTC 2 cut(s) 364, 843
EcoT14I CCWWGG 1 cut(s) 398
EcoT22I ATGCAT 1 cut(s) 657
EcoT38I GRGCYC 2 cut(s) 366, 845
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 398
FaeI CATG 7 cut(s) 161, 212, 341, 370, 402, 442, 556
FalI AAGNNNNNCTT 2 cut(s) 204, 236
FatI CATG 7 cut(s) 157, 208, 337, 366, 398, 438, 552
Fnu4HI GCNGC 5 cut(s) 190, 258, 375, 528, 612
FokI GGATG 3 cut(s) 527, 827, 877
FriOI GRGCYC 2 cut(s) 366, 845
Fsp4HI GCNGC 5 cut(s) 190, 258, 375, 528, 612
GlaI GCGC 1 cut(s) 6
GluI GCNGC 5 cut(s) 190, 258, 375, 528, 612
GsaI CCCAGC 1 cut(s) 381
GsuI CTGGAG 1 cut(s) 815
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 1 cut(s) 527
HapII CCGG 2 cut(s) 454, 499
HhaI GCGC 1 cut(s) 7
Hin1I GRCGYC 2 cut(s) 5, 576
Hin1II CATG 7 cut(s) 161, 212, 341, 370, 402, 442, 556
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HincII GTYRAC 1 cut(s) 184
HindII GTYRAC 1 cut(s) 184
HindIII AAGCTT 2 cut(s) 679, 701
HinfI GANTC 2 cut(s) 406, 507
HpaII CCGG 2 cut(s) 454, 499
HphI GGTGA 4 cut(s) 584, 631, 890, 902
Hpy166II GTNNAC 3 cut(s) 184, 395, 447
Hpy188III TCNNGA 7 cut(s) 83, 338, 367, 454, 488, 572, 794
Hpy8I GTNNAC 3 cut(s) 184, 395, 447
Hpy99I CGWCG 6 cut(s) 494, 578, 581, 584, 608, 611
HpyAV CCTTC 4 cut(s) 35, 347, 457, 578
HpyCH4III ACNGT 1 cut(s) 550
HpyCH4IV ACGT 2 cut(s) 177, 576
HpyCH4V TGCA 7 cut(s) 42, 206, 260, 374, 534, 655, 671
HpyF10VI GCNNNNNNNGC 3 cut(s) 39, 212, 271
HpyF3I CTNAG 4 cut(s) 29, 81, 297, 800
HpySE526I ACGT 2 cut(s) 177, 576
Hsp92I GRCGYC 2 cut(s) 5, 576
Hsp92II CATG 7 cut(s) 161, 212, 341, 370, 402, 442, 556
HspAI GCGC 1 cut(s) 5
KasI GGCGCC 1 cut(s) 4
Kpn2I TCCGGA 1 cut(s) 453
KpnI GGTACC 1 cut(s) 140
Kzo9I GATC 5 cut(s) 151, 536, 618, 707, 716
LmnI GCTCC 4 cut(s) 431, 456, 605, 796
Lsp1109I GCAGC 3 cut(s) 201, 244, 386
LweI GCATC 2 cut(s) 642, 680
MaeII ACGT 2 cut(s) 177, 576
MaeIII GTNAC 3 cut(s) 64, 637, 737
MalI GATC 5 cut(s) 153, 538, 620, 709, 718
MbiI CCGCTC 1 cut(s) 600
MboI GATC 5 cut(s) 151, 536, 618, 707, 716
MboII GAAGA 5 cut(s) 146, 215, 502, 511, 819
MfeI CAATTG 1 cut(s) 911
MflI RGATCY 3 cut(s) 536, 618, 707
MhlI GDGCHC 4 cut(s) 345, 366, 806, 845
MluCI AATT 2 cut(s) 695, 911
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 1 cut(s) 516
Mph1103I ATGCAT 1 cut(s) 657
MroI TCCGGA 1 cut(s) 453
MseI TTAA 2 cut(s) 198, 699
MspA1I CMGCKG 1 cut(s) 377
MspI CCGG 2 cut(s) 454, 499
MspR9I CCNGG 1 cut(s) 499
MunI CAATTG 1 cut(s) 911
MwoI GCNNNNNNNGC 3 cut(s) 39, 212, 271
NarI GGCGCC 1 cut(s) 5
NciI CCSGG 1 cut(s) 499
NcoI CCATGG 1 cut(s) 398
NdeII GATC 5 cut(s) 151, 536, 618, 707, 716
NlaIII CATG 7 cut(s) 161, 212, 341, 370, 402, 442, 556
NlaIV GGNNCC 4 cut(s) 6, 138, 468, 503
NmeAIII GCCGAG 1 cut(s) 640
NmuCI GTSAC 2 cut(s) 637, 737
NsiI ATGCAT 1 cut(s) 657
NspI RCATGY 1 cut(s) 442
PagI TCATGA 2 cut(s) 337, 366
PciI ACATGT 1 cut(s) 438
PcsI WCGNNNNNNNCGW 1 cut(s) 612
PfeI GAWTC 1 cut(s) 406
PflFI GACNNNGTC 1 cut(s) 578
PkrI GCNGC 5 cut(s) 191, 259, 376, 529, 613
Ple19I CGATCG 1 cut(s) 719
PleI GAGTC 1 cut(s) 515
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 1 cut(s) 515
PscI ACATGT 1 cut(s) 438
PshBI ATTAAT 1 cut(s) 198
Psp124BI GAGCTC 2 cut(s) 366, 845
PspFI CCCAGC 1 cut(s) 377
PspN4I GGNNCC 4 cut(s) 6, 138, 468, 503
PspPI GGNCC 1 cut(s) 525
PstI CTGCAG 1 cut(s) 536
PsuI RGATCY 3 cut(s) 536, 618, 707
PsyI GACNNNGTC 1 cut(s) 578
PvuI CGATCG 1 cut(s) 719
PvuII CAGCTG 1 cut(s) 377
RgaI GCGATCGC 1 cut(s) 719
RsaI GTAC 3 cut(s) 138, 394, 823
RsaNI GTAC 3 cut(s) 137, 393, 822
SacI GAGCTC 2 cut(s) 366, 845
SaqAI TTAA 2 cut(s) 198, 699
SatI GCNGC 5 cut(s) 190, 258, 375, 528, 612
Sau3AI GATC 5 cut(s) 151, 536, 618, 707, 716
Sau96I GGNCC 1 cut(s) 525
SchI GAGTC 1 cut(s) 516
ScrFI CCNGG 1 cut(s) 499
SduI GDGCHC 4 cut(s) 345, 366, 806, 845
SfaAI GCGATCGC 1 cut(s) 719
SfaNI GCATC 2 cut(s) 642, 680
SfcI CTRYAG 3 cut(s) 170, 532, 546
SfoI GGCGCC 1 cut(s) 6
SgfI GCGATCGC 1 cut(s) 719
SmlI CTYRAG 1 cut(s) 422
SmoI CTYRAG 1 cut(s) 422
Sse9I AATT 2 cut(s) 695, 911
SsiI CCGC 5 cut(s) 294, 528, 598, 612, 622
SspDI GGCGCC 1 cut(s) 4
SspI AATATT 1 cut(s) 202
SstI GAGCTC 2 cut(s) 366, 845
StyD4I CCNGG 1 cut(s) 497
StyI CCWWGG 1 cut(s) 398
TaaI ACNGT 1 cut(s) 550
TaiI ACGT 2 cut(s) 180, 579
TaqI TCGA 5 cut(s) 13, 413, 489, 513, 772
TaqII GACCGA 1 cut(s) 469
TasI AATT 2 cut(s) 695, 911
TauI GCSGC 2 cut(s) 530, 614
TfiI GAWTC 1 cut(s) 406
Tru1I TTAA 2 cut(s) 198, 699
Tru9I TTAA 2 cut(s) 198, 699
TseFI GTSAC 2 cut(s) 637, 737
TseI GCWGC 3 cut(s) 189, 257, 374
Tsp45I GTSAC 2 cut(s) 637, 737
TspDTI ATGAA 4 cut(s) 146, 225, 673, 881
TspGWI ACGGA 2 cut(s) 592, 625
Tth111I GACNNNGTC 1 cut(s) 578
VspI ATTAAT 1 cut(s) 198
XapI RAATTY 1 cut(s) 695
XceI RCATGY 1 cut(s) 442
ZraI GACGTC 1 cut(s) 577
Zsp2I ATGCAT 1 cut(s) 657
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.