Rh2BG509000

wall-associated receptor kinase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0011615
Physical Location & Seq
Reverse (-)
71532124 .. 71565159
33036 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG509000.1

Sequence Viewer

Length: 789 bp
ATGGCAAAGCCTGGTTGCCCAGAGAAGTGTGGAAATGTTACCATCTCGTACCCTTTCGGGATTGGACCTGATTGTTACTATGACGAATGGTTTCAAATCGACTGCAACGAATCTACCGAGGGGAAGCCTTTCTTGAGGTGTACCAAATTGGAGGTGCTGAATATATCTACCGAAGGATGGGTTCAGGTGTACAGCCCAGTTACATTCTTCTGCGAGGGAAATAATAGTAGCCAACTGGCAGATTTAAGGGACAGCCCTTTCTTCTACTCTCGACGATACAGCAGATTCACTGCAGTGAGTTGCGATTCCTCTGCCCTGTTGAGTTCCGCAGACTCTGTTGTTGGCGGATGCAGGCCGACCCGGCTTGCTCTAATAGCACTTATCCTGCAGTCAAACGGTCGGAAATTTATTGTTCATGCCGCGAGGGGTTTGAAGGAAATCCGTATCTCGGGGAAAAATCTTGTGATGATATTAATGAATGTCAAGATGGTCGCCGCCCATGCTTTGGCAACAGCAAAGGAAGTGAAATCGATGGGTGATTTCATGGTATTTGAGTTACGAAGCCTGGGACCTTGTACAATGTTCAGTGGCTCTGCAGCCTGTGGAGTTCATGTGCAAAGGGGATCAATTTCTGTACCCAGCAAGAGAATGAGATTGCTGTACCGTATTATCAAAACTTTTAAGGCATTCTTCACGAGCTCTGATCTCATGTTGCTTGTCTTTGACCACATGCAACAAGAACCAATTATCAAAACTTTCCAGCTGTTTAGAGGATATATTTCTAGATAA

Protein Analysis

262

Amino Acids

29.26

Weight (kDa)

9.19

Isoelectric Point (pI)

47.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 6 - 59 5e-13 Wall-associated receptor kinase galacturonan-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 505
AccII CGCG 1 cut(s) 422
AciI CCGC 4 cut(s) 327, 345, 420, 495
AclWI GGATC 1 cut(s) 631
AcsI RAATTY 1 cut(s) 404
AfaI GTAC 6 cut(s) 50, 142, 191, 577, 636, 662
AfiI CCNNNNNNNGG 3 cut(s) 177, 448, 505
AgsI TTSAA 2 cut(s) 95, 433
AjnI CCWGG 2 cut(s) 10, 564
AleI CACNNNNGTG 1 cut(s) 293
AluBI AGCT 2 cut(s) 699, 763
AluI AGCT 2 cut(s) 699, 763
Alw21I GWGCWC 1 cut(s) 701
AlwI GGATC 1 cut(s) 631
AlwNI CAGNNNCTG 1 cut(s) 335
Ama87I CYCGRG 1 cut(s) 448
AoxI GGCC 1 cut(s) 353
ApeKI GCWGC 1 cut(s) 596
ApoI RAATTY 1 cut(s) 404
AseI ATTAAT 1 cut(s) 473
Asp700I GAANNNNTTC 2 cut(s) 90, 128
AspS9I GGNCC 2 cut(s) 65, 569
AsuC2I CCSGG 1 cut(s) 361
AsuHPI GGTGA 1 cut(s) 548
AvaI CYCGRG 1 cut(s) 448
AvaII GGWCC 2 cut(s) 65, 569
BanII GRGCYC 1 cut(s) 701
BauI CACGAG 1 cut(s) 694
Bbv12I GWGCWC 1 cut(s) 701
BbvI GCAGC 1 cut(s) 608
BccI CCATC 4 cut(s) 50, 171, 481, 526
BcgI CGANNNNNNTGC 2 cut(s) 293, 327
BciT130I CCWGG 2 cut(s) 12, 566
BcnI CCSGG 1 cut(s) 361
BfaI CTAG 1 cut(s) 783
BfmI CTRYAG 3 cut(s) 291, 386, 594
BglI GCCNNNNNGGC 1 cut(s) 361
BisI GCNGC 3 cut(s) 420, 495, 597
BlsI GCNGC 3 cut(s) 421, 496, 598
Bme1390I CCNGG 3 cut(s) 12, 361, 566
Bme18I GGWCC 2 cut(s) 65, 569
BmeT110I CYCGRG 1 cut(s) 448
BmgT120I GGNCC 2 cut(s) 65, 569
BmiI GGNNCC 1 cut(s) 570
BmrFI CCNGG 3 cut(s) 12, 361, 566
BmrI ACTGGG 1 cut(s) 191
BmsI GCATC 1 cut(s) 338
BmuI ACTGGG 1 cut(s) 191
BpuEI CTTGAG 1 cut(s) 154
BpuMI CCSGG 1 cut(s) 361
Bsa29I ATCGAT 1 cut(s) 530
BsaJI CCNNGG 2 cut(s) 117, 565
BsaXI ACNNNNNCTCC 2 cut(s) 597, 627
Bsc4I CCNNNNNNNGG 3 cut(s) 177, 448, 505
Bse1I ACTGG 2 cut(s) 197, 240
BseBI CCWGG 2 cut(s) 12, 566
BseCI ATCGAT 1 cut(s) 530
BseDI CCNNGG 2 cut(s) 117, 565
BseGI GGATG 2 cut(s) 182, 353
BseLI CCNNNNNNNGG 3 cut(s) 177, 448, 505
BseNI ACTGG 2 cut(s) 197, 240
BseXI GCAGC 1 cut(s) 608
BseYI CCCAGC 1 cut(s) 638
Bsh1236I CGCG 1 cut(s) 422
Bsh1285I CGRYCG 1 cut(s) 400
BshFI GGCC 1 cut(s) 355
BshVI ATCGAT 1 cut(s) 530
BsiEI CGRYCG 1 cut(s) 400
BsiHKAI GWGCWC 1 cut(s) 701
BsiHKCI CYCGRG 1 cut(s) 448
BsiSI CCGG 1 cut(s) 361
BslFI GGGAC 2 cut(s) 263, 582
BslI CCNNNNNNNGG 3 cut(s) 177, 448, 505
BsmFI GGGAC 2 cut(s) 263, 582
BsmI GAATGC 1 cut(s) 686
BsnI GGCC 1 cut(s) 355
BsoBI CYCGRG 1 cut(s) 448
Bsp1286I GDGCHC 1 cut(s) 701
Bsp1407I TGTACA 2 cut(s) 189, 575
Bsp143I GATC 2 cut(s) 623, 703
BspACI CCGC 4 cut(s) 327, 345, 420, 495
BspANI GGCC 1 cut(s) 355
BspDI ATCGAT 1 cut(s) 530
BspFNI CGCG 1 cut(s) 422
BspLI GGNNCC 1 cut(s) 570
BspMAI CTGCAG 3 cut(s) 295, 390, 598
BspPI GGATC 1 cut(s) 631
BsrGI TGTACA 2 cut(s) 189, 575
BsrI ACTGG 2 cut(s) 197, 240
BssECI CCNNGG 2 cut(s) 117, 565
BssMI GATC 2 cut(s) 623, 703
BssSI CACGAG 1 cut(s) 694
Bst2BI CACGAG 1 cut(s) 694
Bst2UI CCWGG 2 cut(s) 12, 566
Bst4CI ACNGT 2 cut(s) 398, 665
BstAUI TGTACA 2 cut(s) 189, 575
BstC8I GCNNGC 2 cut(s) 353, 366
BstF5I GGATG 2 cut(s) 182, 353
BstFNI CGCG 1 cut(s) 422
BstKTI GATC 2 cut(s) 626, 706
BstMBI GATC 2 cut(s) 623, 703
BstMCI CGRYCG 1 cut(s) 400
BstMWI GCNNNNNNNGC 3 cut(s) 361, 374, 500
BstNI CCWGG 2 cut(s) 12, 566
BstNSI RCATGY 1 cut(s) 733
BstSCI CCNGG 3 cut(s) 10, 359, 564
BstSFI CTRYAG 3 cut(s) 291, 386, 594
BstUI CGCG 1 cut(s) 422
BstV1I GCAGC 1 cut(s) 608
Bsu15I ATCGAT 1 cut(s) 530
BsuRI GGCC 1 cut(s) 355
BsuTUI ATCGAT 1 cut(s) 530
BtsCI GGATG 2 cut(s) 182, 353
BtsI GCAGTG 2 cut(s) 288, 300
BtsIMutI CAGTG 3 cut(s) 288, 300, 592
Cac8I GCNNGC 2 cut(s) 353, 366
CaiI CAGNNNCTG 1 cut(s) 335
Cfr13I GGNCC 2 cut(s) 65, 569
ClaI ATCGAT 1 cut(s) 530
Csp6I GTAC 6 cut(s) 49, 141, 190, 576, 635, 661
CviAII CATG 6 cut(s) 416, 500, 544, 611, 709, 730
CviQI GTAC 6 cut(s) 49, 141, 190, 576, 635, 661
DpnI GATC 2 cut(s) 625, 705
DpnII GATC 2 cut(s) 623, 703
EciI GGCGGA 1 cut(s) 360
Ecl136II GAGCTC 1 cut(s) 699
Eco24I GRGCYC 1 cut(s) 701
Eco47I GGWCC 2 cut(s) 65, 569
Eco53kI GAGCTC 1 cut(s) 699
Eco88I CYCGRG 1 cut(s) 448
EcoICRI GAGCTC 1 cut(s) 699
EcoO109I RGGNCCY 1 cut(s) 569
EcoRII CCWGG 2 cut(s) 10, 564
EcoT38I GRGCYC 1 cut(s) 701
FaeI CATG 6 cut(s) 419, 503, 547, 614, 712, 733
FaiI YATR 9 cut(s) 81, 164, 417, 501, 545, 612, 710, 731, 777
FalI AAGNNNNNCTT 4 cut(s) 116, 148, 674, 706
FaqI GGGAC 2 cut(s) 263, 582
FatI CATG 6 cut(s) 415, 499, 543, 610, 708, 729
Fnu4HI GCNGC 3 cut(s) 420, 495, 597
FokI GGATG 2 cut(s) 189, 360
FriOI GRGCYC 1 cut(s) 701
Fsp4HI GCNGC 3 cut(s) 420, 495, 597
FspBI CTAG 1 cut(s) 783
GluI GCNGC 3 cut(s) 420, 495, 597
GsaI CCCAGC 1 cut(s) 642
HaeIII GGCC 1 cut(s) 355
HapII CCGG 1 cut(s) 361
Hin1II CATG 6 cut(s) 419, 503, 547, 614, 712, 733
HinfI GANTC 4 cut(s) 110, 285, 305, 332
HpaII CCGG 1 cut(s) 361
HphI GGTGA 1 cut(s) 548
Hpy166II GTNNAC 2 cut(s) 141, 190
Hpy188I TCNGA 2 cut(s) 402, 703
Hpy188III TCNNGA 6 cut(s) 58, 133, 270, 484, 694, 783
Hpy8I GTNNAC 2 cut(s) 141, 190
Hpy99I CGWCG 1 cut(s) 276
HpyAV CCTTC 2 cut(s) 167, 427
HpyCH4III ACNGT 2 cut(s) 398, 665
HpyCH4V TGCA 7 cut(s) 105, 293, 351, 388, 596, 616, 733
HpyF10VI GCNNNNNNNGC 3 cut(s) 361, 374, 500
Hsp92II CATG 6 cut(s) 419, 503, 547, 614, 712, 733
Kzo9I GATC 2 cut(s) 623, 703
Lsp1109I GCAGC 1 cut(s) 608
LweI GCATC 1 cut(s) 338
MaeI CTAG 1 cut(s) 783
MaeIII GTNAC 4 cut(s) 37, 74, 199, 555
MalI GATC 2 cut(s) 625, 705
MboI GATC 2 cut(s) 623, 703
MboII GAAGA 3 cut(s) 199, 253, 682
MhlI GDGCHC 1 cut(s) 701
MluCI AATT 4 cut(s) 146, 404, 627, 744
MlyI GAGTC 1 cut(s) 326
MmeI TCCRAC 1 cut(s) 380
MnlI CCTC 7 cut(s) 112, 129, 145, 208, 319, 417, 764
MroXI GAANNNNTTC 2 cut(s) 90, 128
MseI TTAA 3 cut(s) 245, 473, 681
MslI CAYNNNNRTG 1 cut(s) 293
MspA1I CMGCKG 1 cut(s) 763
MspI CCGG 1 cut(s) 361
MspR9I CCNGG 3 cut(s) 12, 361, 566
Mva1269I GAATGC 1 cut(s) 686
MvaI CCWGG 2 cut(s) 12, 566
MvnI CGCG 1 cut(s) 422
MwoI GCNNNNNNNGC 3 cut(s) 361, 374, 500
NciI CCSGG 1 cut(s) 361
NdeII GATC 2 cut(s) 623, 703
NlaIII CATG 6 cut(s) 419, 503, 547, 614, 712, 733
NlaIV GGNNCC 1 cut(s) 570
NspI RCATGY 1 cut(s) 733
OliI CACNNNNGTG 1 cut(s) 293
PcsI WCGNNNNNNNCGW 2 cut(s) 105, 114
PctI GAATGC 1 cut(s) 686
PdmI GAANNNNTTC 2 cut(s) 90, 128
PfeI GAWTC 3 cut(s) 110, 285, 305
PflMI CCANNNNNTGG 1 cut(s) 505
PkrI GCNGC 3 cut(s) 421, 496, 598
PleI GAGTC 1 cut(s) 326
PpsI GAGTC 1 cut(s) 326
PpuMI RGGWCCY 1 cut(s) 569
PshBI ATTAAT 1 cut(s) 473
Psp124BI GAGCTC 1 cut(s) 701
Psp5II RGGWCCY 1 cut(s) 569
Psp6I CCWGG 2 cut(s) 10, 564
PspFI CCCAGC 1 cut(s) 638
PspGI CCWGG 2 cut(s) 10, 564
PspN4I GGNNCC 1 cut(s) 570
PspPI GGNCC 2 cut(s) 65, 569
PspPPI RGGWCCY 1 cut(s) 569
PstI CTGCAG 3 cut(s) 295, 390, 598
PstNI CAGNNNCTG 1 cut(s) 335
PvuII CAGCTG 1 cut(s) 763
RsaI GTAC 6 cut(s) 50, 142, 191, 577, 636, 662
RsaNI GTAC 6 cut(s) 49, 141, 190, 576, 635, 661
RseI CAYNNNNRTG 1 cut(s) 293
SacI GAGCTC 1 cut(s) 701
SaqAI TTAA 3 cut(s) 245, 473, 681
SatI GCNGC 3 cut(s) 420, 495, 597
Sau3AI GATC 2 cut(s) 623, 703
Sau96I GGNCC 2 cut(s) 65, 569
SchI GAGTC 1 cut(s) 326
ScrFI CCNGG 3 cut(s) 12, 361, 566
SduI GDGCHC 1 cut(s) 701
SetI ASST 7 cut(s) 70, 140, 156, 189, 574, 701, 765
SfaNI GCATC 1 cut(s) 338
SfcI CTRYAG 3 cut(s) 291, 386, 594
SinI GGWCC 2 cut(s) 65, 569
SmiMI CAYNNNNRTG 1 cut(s) 293
SmlI CTYRAG 1 cut(s) 133
SmoI CTYRAG 1 cut(s) 133
Sse9I AATT 4 cut(s) 146, 404, 627, 744
SsiI CCGC 4 cut(s) 327, 345, 420, 495
SspMI CTAG 1 cut(s) 783
SstI GAGCTC 1 cut(s) 701
StyD4I CCNGG 3 cut(s) 10, 359, 564
TaaI ACNGT 2 cut(s) 398, 665
TaqI TCGA 3 cut(s) 99, 271, 530
TasI AATT 4 cut(s) 146, 404, 627, 744
TatI WGTACW 2 cut(s) 189, 575
TauI GCSGC 2 cut(s) 422, 497
TfiI GAWTC 3 cut(s) 110, 285, 305
Tru1I TTAA 3 cut(s) 245, 473, 681
Tru9I TTAA 3 cut(s) 245, 473, 681
TscAI CASTG 3 cut(s) 295, 300, 592
TseI GCWGC 1 cut(s) 596
TspDTI ATGAA 4 cut(s) 404, 491, 532, 599
TspGWI ACGGA 1 cut(s) 431
TspRI CASTG 3 cut(s) 295, 300, 592
Van91I CCANNNNNTGG 1 cut(s) 505
VpaK11BI GGWCC 2 cut(s) 65, 569
VspI ATTAAT 1 cut(s) 473
XapI RAATTY 1 cut(s) 404
XbaI TCTAGA 1 cut(s) 782
XceI RCATGY 1 cut(s) 733
XmnI GAANNNNTTC 2 cut(s) 90, 128
XspI CTAG 1 cut(s) 783
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.