P5CS plays a key role in proline biosynthesis, leading to osmoregulation in plants
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| Species | Orthologous Gene IDs |
|---|---|
| rosa_chinensis | RchiOBHm_Chr6g0299761 |
| rosa_roxburghii | Rroxscaffold_1G00029190 |
| rosa_samantha | Rh1CG186800 Rh2AG392000 Rh2BG097000 Rh2CG099100 Rh2CG378800 Rh2DG096500 Rh4CG209500 Rh5AG382700 Rh6DG310100 Rh7AG468000 Rh7CG085900 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 1 cut(s) | 104 |
| AcvI | CACGTG | 1 cut(s) | 155 |
| AfaI | GTAC | 1 cut(s) | 328 |
| AgsI | TTSAA | 3 cut(s) | 109, 248, 307 |
| AluBI | AGCT | 5 cut(s) | 203, 256, 389, 398, 406 |
| AluI | AGCT | 5 cut(s) | 203, 256, 389, 398, 406 |
| Alw26I | GTCTC | 1 cut(s) | 452 |
| AoxI | GGCC | 1 cut(s) | 458 |
| ApeKI | GCWGC | 2 cut(s) | 253, 386 |
| ApoI | RAATTY | 1 cut(s) | 104 |
| AspS9I | GGNCC | 1 cut(s) | 458 |
| BbrPI | CACGTG | 1 cut(s) | 155 |
| BbvI | GCAGC | 2 cut(s) | 265, 398 |
| BcoDI | GTCTC | 1 cut(s) | 452 |
| BfaI | CTAG | 2 cut(s) | 66, 395 |
| BfmI | CTRYAG | 1 cut(s) | 451 |
| BisI | GCNGC | 2 cut(s) | 254, 387 |
| BlsI | GCNGC | 2 cut(s) | 255, 388 |
| BmgT120I | GGNCC | 1 cut(s) | 458 |
| BmsI | GCATC | 1 cut(s) | 307 |
| Bpu10I | CCTNAGC | 1 cut(s) | 399 |
| BsaAI | YACGTR | 1 cut(s) | 155 |
| BsaI | GGTCTC | 1 cut(s) | 452 |
| BseGI | GGATG | 2 cut(s) | 8, 196 |
| BseMII | CTCAG | 1 cut(s) | 90 |
| BseRI | GAGGAG | 1 cut(s) | 5 |
| BseXI | GCAGC | 2 cut(s) | 265, 398 |
| BshFI | GGCC | 1 cut(s) | 460 |
| BslFI | GGGAC | 1 cut(s) | 383 |
| BsmAI | GTCTC | 1 cut(s) | 452 |
| BsmFI | GGGAC | 1 cut(s) | 383 |
| BsmI | GAATGC | 1 cut(s) | 61 |
| BsnI | GGCC | 1 cut(s) | 460 |
| Bso31I | GGTCTC | 1 cut(s) | 452 |
| Bsp143I | GATC | 2 cut(s) | 271, 415 |
| BspANI | GGCC | 1 cut(s) | 460 |
| BspCNI | CTCAG | 1 cut(s) | 91 |
| BspTNI | GGTCTC | 1 cut(s) | 452 |
| BssMI | GATC | 2 cut(s) | 271, 415 |
| Bst4CI | ACNGT | 4 cut(s) | 81, 268, 380, 455 |
| BstBAI | YACGTR | 1 cut(s) | 155 |
| BstDEI | CTNAG | 2 cut(s) | 99, 399 |
| BstF5I | GGATG | 2 cut(s) | 8, 196 |
| BstKTI | GATC | 2 cut(s) | 274, 418 |
| BstMAI | GTCTC | 1 cut(s) | 452 |
| BstMBI | GATC | 2 cut(s) | 271, 415 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 395 |
| BstSFI | CTRYAG | 1 cut(s) | 451 |
| BstV1I | GCAGC | 2 cut(s) | 265, 398 |
| BsuRI | GGCC | 1 cut(s) | 460 |
| BtsCI | GGATG | 2 cut(s) | 8, 196 |
| BtsIMutI | CAGTG | 1 cut(s) | 460 |
| Cfr13I | GGNCC | 1 cut(s) | 458 |
| Csp6I | GTAC | 1 cut(s) | 327 |
| CviAII | CATG | 1 cut(s) | 32 |
| CviJI | RGCY | 7 cut(s) | 73, 203, 256, 389, 398, 406, 460 |
| CviKI_1 | RGCY | 7 cut(s) | 73, 203, 256, 389, 398, 406, 460 |
| CviQI | GTAC | 1 cut(s) | 327 |
| DdeI | CTNAG | 2 cut(s) | 99, 399 |
| DpnI | GATC | 2 cut(s) | 273, 417 |
| DpnII | GATC | 2 cut(s) | 271, 415 |
| DraI | TTTAAA | 1 cut(s) | 288 |
| Eco31I | GGTCTC | 1 cut(s) | 452 |
| Eco72I | CACGTG | 1 cut(s) | 155 |
| EcoRI | GAATTC | 1 cut(s) | 104 |
| FaeI | CATG | 1 cut(s) | 35 |
| FaiI | YATR | 8 cut(s) | 33, 171, 296, 302, 343, 362, 392, 431 |
| FaqI | GGGAC | 1 cut(s) | 383 |
| FatI | CATG | 1 cut(s) | 31 |
| Fnu4HI | GCNGC | 2 cut(s) | 254, 387 |
| FokI | GGATG | 1 cut(s) | 203 |
| Fsp4HI | GCNGC | 2 cut(s) | 254, 387 |
| FspBI | CTAG | 2 cut(s) | 66, 395 |
| GluI | GCNGC | 2 cut(s) | 254, 387 |
| HaeIII | GGCC | 1 cut(s) | 460 |
| Hin1II | CATG | 1 cut(s) | 35 |
| HinfI | GANTC | 3 cut(s) | 113, 127, 350 |
| Hpy188I | TCNGA | 2 cut(s) | 100, 142 |
| Hpy188III | TCNNGA | 3 cut(s) | 66, 188, 212 |
| Hpy99I | CGWCG | 1 cut(s) | 272 |
| HpyAV | CCTTC | 1 cut(s) | 32 |
| HpyCH4III | ACNGT | 4 cut(s) | 81, 268, 380, 455 |
| HpyCH4IV | ACGT | 1 cut(s) | 154 |
| HpyCH4V | TGCA | 2 cut(s) | 61, 320 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 395 |
| HpyF3I | CTNAG | 2 cut(s) | 99, 399 |
| HpySE526I | ACGT | 1 cut(s) | 154 |
| Hsp92II | CATG | 1 cut(s) | 35 |
| Kzo9I | GATC | 2 cut(s) | 271, 415 |
| LmnI | GCTCC | 1 cut(s) | 403 |
| LpnPI | CCDG | 3 cut(s) | 173, 342, 343 |
| Lsp1109I | GCAGC | 2 cut(s) | 265, 398 |
| LweI | GCATC | 1 cut(s) | 307 |
| MaeI | CTAG | 2 cut(s) | 66, 395 |
| MaeII | ACGT | 1 cut(s) | 154 |
| MaeIII | GTNAC | 5 cut(s) | 4, 133, 172, 193, 224 |
| MalI | GATC | 2 cut(s) | 273, 417 |
| MboI | GATC | 2 cut(s) | 271, 415 |
| MboII | GAAGA | 3 cut(s) | 17, 345, 410 |
| MfeI | CAATTG | 1 cut(s) | 122 |
| MluCI | AATT | 2 cut(s) | 104, 122 |
| MnlI | CCTC | 9 cut(s) | 23, 26, 49, 84, 94, 148, 340, 436, 471 |
| MseI | TTAA | 1 cut(s) | 287 |
| MspA1I | CMGCKG | 1 cut(s) | 256 |
| MunI | CAATTG | 1 cut(s) | 122 |
| Mva1269I | GAATGC | 1 cut(s) | 61 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 395 |
| NdeII | GATC | 2 cut(s) | 271, 415 |
| NlaIII | CATG | 1 cut(s) | 35 |
| NmuCI | GTSAC | 2 cut(s) | 193, 224 |
| PctI | GAATGC | 1 cut(s) | 61 |
| PfeI | GAWTC | 3 cut(s) | 113, 127, 350 |
| PkrI | GCNGC | 2 cut(s) | 255, 388 |
| PmaCI | CACGTG | 1 cut(s) | 155 |
| PmlI | CACGTG | 1 cut(s) | 155 |
| Ppu21I | YACGTR | 1 cut(s) | 155 |
| PspCI | CACGTG | 1 cut(s) | 155 |
| PspPI | GGNCC | 1 cut(s) | 458 |
| PvuII | CAGCTG | 1 cut(s) | 256 |
| RsaI | GTAC | 1 cut(s) | 328 |
| RsaNI | GTAC | 1 cut(s) | 327 |
| SaqAI | TTAA | 1 cut(s) | 287 |
| SatI | GCNGC | 2 cut(s) | 254, 387 |
| Sau3AI | GATC | 2 cut(s) | 271, 415 |
| Sau96I | GGNCC | 1 cut(s) | 458 |
| SfaNI | GCATC | 1 cut(s) | 307 |
| SfcI | CTRYAG | 1 cut(s) | 451 |
| Sse9I | AATT | 2 cut(s) | 104, 122 |
| SspMI | CTAG | 2 cut(s) | 66, 395 |
| TaaI | ACNGT | 4 cut(s) | 81, 268, 380, 455 |
| TaiI | ACGT | 1 cut(s) | 157 |
| TaqI | TCGA | 2 cut(s) | 85, 270 |
| TasI | AATT | 2 cut(s) | 104, 122 |
| TfiI | GAWTC | 3 cut(s) | 113, 127, 350 |
| Tru1I | TTAA | 1 cut(s) | 287 |
| Tru9I | TTAA | 1 cut(s) | 287 |
| TscAI | CASTG | 1 cut(s) | 460 |
| TseFI | GTSAC | 2 cut(s) | 193, 224 |
| TseI | GCWGC | 2 cut(s) | 253, 386 |
| Tsp45I | GTSAC | 2 cut(s) | 193, 224 |
| TspDTI | ATGAA | 4 cut(s) | 53, 59, 176, 324 |
| TspRI | CASTG | 1 cut(s) | 460 |
| XapI | RAATTY | 1 cut(s) | 104 |
| XbaI | TCTAGA | 1 cut(s) | 65 |
| XspI | CTAG | 2 cut(s) | 66, 395 |