Rh2DG096500

DEAD-box ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0017446
Physical Location & Seq
Forward (+)
7584674 .. 7586304
1631 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG096500.1

Sequence Viewer

Length: 504 bp
ATGATTATGGGCATCATGATCCCATCTCTACTTGCTGTGCAGGTTAAGCAGTCATATCTTGTTGCACCACATGACTTGCATTTTCAAATAGTTCACCATCTTTTGAAGGAGCATATCTGGCAGTCACCTGATTATAAGGTTATTGTTTTCTGTACAACAGGAATGGTAACATCCCTCCTCTACCTTCTTCTCCGTGAAATGAAAATGAATGTTAGGGAGATACATTCTAGAAAGCCTCAAATGTATCAAACTCGTATCTCTGAGGAATTCAAAGAATCAAAGCAATTAATTTTTGTTACCTCTGATGTATCAGGACTATATGTTGGTGATAAAGAATATAGGTGCAGCTTACGCAGGACAATATATGAGCATGTGAAAGTGCATTCCTCAGTATCTGATGTAAGAATGCTATGTGAGAAAGAATATAGGTGCAAGCTCTTGCCCAGTAATGTTGAATTGTTTGTAACTGATCGAGTAGAGGAGAGTAACAACCATCAAGGTTGA

Protein Analysis

167

Amino Acids

19.64

Weight (kDa)

8.32

Isoelectric Point (pI)

37.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helicase_C PF00271 34 - 104 6.8e-07 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 135
Acc36I ACCTGC 1 cut(s) 31
AclWI GGATC 1 cut(s) 13
AcsI RAATTY 1 cut(s) 266
AfaI GTAC 1 cut(s) 154
AgsI TTSAA 4 cut(s) 86, 106, 271, 455
AluBI AGCT 2 cut(s) 348, 436
AluI AGCT 2 cut(s) 348, 436
AlwI GGATC 1 cut(s) 13
AlwNI CAGNNNCTG 1 cut(s) 395
ApeKI GCWGC 1 cut(s) 345
ApoI RAATTY 1 cut(s) 266
AseI ATTAAT 1 cut(s) 287
AsuHPI GGTGA 3 cut(s) 86, 117, 338
BbvI GCAGC 1 cut(s) 357
BccI CCATC 3 cut(s) 31, 105, 501
BfaI CTAG 1 cut(s) 228
BfuAI ACCTGC 1 cut(s) 31
BisI GCNGC 1 cut(s) 346
BlsI GCNGC 1 cut(s) 347
BmrI ACTGGG 1 cut(s) 438
BmsI GCATC 1 cut(s) 21
BmuI ACTGGG 1 cut(s) 438
Bse1I ACTGG 1 cut(s) 444
BseGI GGATG 1 cut(s) 170
BseMII CTCAG 2 cut(s) 252, 402
BseNI ACTGG 1 cut(s) 444
BseRI GAGGAG 2 cut(s) 167, 494
BseXI GCAGC 1 cut(s) 357
BsgI GTGCAG 2 cut(s) 59, 364
BsmI GAATGC 2 cut(s) 382, 411
Bsp1407I TGTACA 1 cut(s) 152
Bsp143I GATC 2 cut(s) 18, 469
BspCNI CTCAG 2 cut(s) 253, 401
BspHI TCATGA 1 cut(s) 15
BspMI ACCTGC 1 cut(s) 31
BspPI GGATC 1 cut(s) 13
BsrGI TGTACA 1 cut(s) 152
BsrI ACTGG 1 cut(s) 444
BssMI GATC 2 cut(s) 18, 469
BstAUI TGTACA 1 cut(s) 152
BstC8I GCNNGC 1 cut(s) 434
BstDEI CTNAG 2 cut(s) 261, 388
BstF5I GGATG 1 cut(s) 170
BstKTI GATC 2 cut(s) 21, 472
BstMBI GATC 2 cut(s) 18, 469
BstMWI GCNNNNNNNGC 3 cut(s) 46, 118, 351
BstNSI RCATGY 1 cut(s) 374
BstV1I GCAGC 1 cut(s) 357
BtsCI GGATG 1 cut(s) 170
BveI ACCTGC 1 cut(s) 31
Cac8I GCNNGC 1 cut(s) 434
CaiI CAGNNNCTG 1 cut(s) 395
CciI TCATGA 1 cut(s) 15
Csp6I GTAC 1 cut(s) 153
CspCI CAANNNNNGTGG 2 cut(s) 57, 92
CviAII CATG 3 cut(s) 16, 71, 371
CviJI RGCY 3 cut(s) 235, 348, 436
CviKI_1 RGCY 3 cut(s) 235, 348, 436
CviQI GTAC 1 cut(s) 153
DdeI CTNAG 2 cut(s) 261, 388
DpnI GATC 2 cut(s) 20, 471
DpnII GATC 2 cut(s) 18, 469
EcoRI GAATTC 1 cut(s) 266
FaeI CATG 3 cut(s) 19, 74, 374
FatI CATG 3 cut(s) 15, 70, 370
Fnu4HI GCNGC 1 cut(s) 346
FokI GGATG 1 cut(s) 157
Fsp4HI GCNGC 1 cut(s) 346
FspBI CTAG 1 cut(s) 228
GluI GCNGC 1 cut(s) 346
Hin1II CATG 3 cut(s) 19, 74, 374
HinfI GANTC 1 cut(s) 275
HphI GGTGA 3 cut(s) 86, 117, 338
Hpy166II GTNNAC 1 cut(s) 94
Hpy188I TCNGA 3 cut(s) 262, 304, 397
Hpy188III TCNNGA 3 cut(s) 16, 228, 312
Hpy8I GTNNAC 1 cut(s) 94
HpyAV CCTTC 2 cut(s) 100, 194
HpyCH4V TGCA 6 cut(s) 40, 65, 79, 345, 382, 432
HpyF10VI GCNNNNNNNGC 3 cut(s) 46, 118, 351
HpyF3I CTNAG 2 cut(s) 261, 388
Hsp92II CATG 3 cut(s) 19, 74, 374
Kzo9I GATC 2 cut(s) 18, 469
LmnI GCTCC 1 cut(s) 109
LpnPI CCDG 7 cut(s) 26, 103, 141, 144, 297, 340, 457
Lsp1109I GCAGC 1 cut(s) 357
LweI GCATC 1 cut(s) 21
MaeI CTAG 1 cut(s) 228
MaeIII GTNAC 5 cut(s) 123, 166, 295, 463, 485
MalI GATC 2 cut(s) 20, 471
MboI GATC 2 cut(s) 18, 469
MboII GAAGA 1 cut(s) 179
MluCI AATT 4 cut(s) 266, 284, 288, 455
MnlI CCTC 7 cut(s) 185, 188, 246, 256, 310, 397, 472
MseI TTAA 2 cut(s) 45, 287
Mva1269I GAATGC 2 cut(s) 382, 411
MwoI GCNNNNNNNGC 3 cut(s) 46, 118, 351
NdeII GATC 2 cut(s) 18, 469
NlaIII CATG 3 cut(s) 19, 74, 374
NmuCI GTSAC 1 cut(s) 123
NspI RCATGY 1 cut(s) 374
PagI TCATGA 1 cut(s) 15
PctI GAATGC 2 cut(s) 382, 411
PfeI GAWTC 1 cut(s) 275
PkrI GCNGC 1 cut(s) 347
PshBI ATTAAT 1 cut(s) 287
PsiI TTATAA 1 cut(s) 135
PstNI CAGNNNCTG 1 cut(s) 395
RsaI GTAC 1 cut(s) 154
RsaNI GTAC 1 cut(s) 153
SaqAI TTAA 2 cut(s) 45, 287
SatI GCNGC 1 cut(s) 346
Sau3AI GATC 2 cut(s) 18, 469
SfaNI GCATC 1 cut(s) 21
Sse9I AATT 4 cut(s) 266, 284, 288, 455
SspMI CTAG 1 cut(s) 228
TaqI TCGA 1 cut(s) 472
TasI AATT 4 cut(s) 266, 284, 288, 455
TatI WGTACW 1 cut(s) 152
TfiI GAWTC 1 cut(s) 275
Tru1I TTAA 2 cut(s) 45, 287
Tru9I TTAA 2 cut(s) 45, 287
TseFI GTSAC 1 cut(s) 123
TseI GCWGC 1 cut(s) 345
Tsp45I GTSAC 1 cut(s) 123
TspDTI ATGAA 2 cut(s) 215, 221
TspGWI ACGGA 1 cut(s) 182
VspI ATTAAT 1 cut(s) 287
XapI RAATTY 1 cut(s) 266
XbaI TCTAGA 1 cut(s) 227
XceI RCATGY 1 cut(s) 374
XspI CTAG 1 cut(s) 228
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.