Rh2CG617500

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0013715
Physical Location & Seq
Reverse (-)
79304625 .. 79313138
8514 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG617500.1

Sequence Viewer

Length: 462 bp
ATGATGGTGACACAGCAACAAGAGAAGCGAAAGAGGAGATTGGTTTGGATCCTAAGCTTTTTGGGTTTGATGCCATACATCTATGCACATAAGGCATTCCAAAATGTAGGAAGTTCTAATGGGAAAGAGAGTGGAGGGAAAGTAGTCACAGTAGCACTCATAATGTATACAGAATTTGCTGGGGCAGTTCCTACTAGACCTGCACAGGACAGTCAGGACTTGGCATTTTCAGTTTCAAGGTTTATACAAAATGGTGGTTCTTTTGCCAACTACTACATGTACCATGGAGGAACGAATTTTGGCCGAACTGCTAATATGGTATGCAATCTGGCAGAAAGAGTGATCCCAGGAATTGAGTTGGTGCGGTTAGCATCATGGTGTTTGCAGTATGAACCTCGTGAGAGGCCAAATGCCAAGTCTCTTGTGACTGCCCTTTCCTTGTTCAAATCGGATCACAATTAA

Protein Analysis

153

Amino Acids

17.16

Weight (kDa)

9.65

Isoelectric Point (pI)

48.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_35 PF01301 67 - 105 9.8e-10 Glycosyl hydrolases family 35
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 208
AccI GTMKAC 1 cut(s) 167
AciI CCGC 1 cut(s) 364
AclWI GGATC 4 cut(s) 43, 56, 337, 459
AcoI YGGCCR 1 cut(s) 301
AcsI RAATTY 2 cut(s) 173, 295
AfaI GTAC 1 cut(s) 281
AflIII ACRYGT 1 cut(s) 276
AgsI TTSAA 2 cut(s) 237, 445
AjnI CCWGG 1 cut(s) 346
AluBI AGCT 1 cut(s) 57
AluI AGCT 1 cut(s) 57
Alw26I GTCTC 1 cut(s) 423
AlwI GGATC 4 cut(s) 43, 56, 337, 459
AoxI GGCC 2 cut(s) 301, 404
ApoI RAATTY 2 cut(s) 173, 295
ArsI GACNNNNNNTTYG 2 cut(s) 401, 433
AsuHPI GGTGA 1 cut(s) 19
BamHI GGATCC 1 cut(s) 48
BauI CACGAG 1 cut(s) 396
BciT130I CCWGG 1 cut(s) 348
BcoDI GTCTC 1 cut(s) 423
BfaI CTAG 1 cut(s) 195
BfuAI ACCTGC 1 cut(s) 208
Bme1390I CCNGG 1 cut(s) 348
BmiI GGNNCC 1 cut(s) 50
BmrFI CCNGG 1 cut(s) 348
BmsI GCATC 2 cut(s) 60, 380
Bpu10I CCTNAGC 1 cut(s) 53
BsaJI CCNNGG 2 cut(s) 283, 346
BsaXI ACNNNNNCTCC 2 cut(s) 126, 156
BseBI CCWGG 1 cut(s) 348
BseDI CCNNGG 2 cut(s) 283, 346
BseRI GAGGAG 1 cut(s) 49
BseYI CCCAGC 1 cut(s) 179
BsgI GTGCAG 1 cut(s) 186
BshFI GGCC 2 cut(s) 303, 406
BsmAI GTCTC 1 cut(s) 423
BsmI GAATGC 1 cut(s) 95
BsnI GGCC 2 cut(s) 303, 406
Bsp143I GATC 3 cut(s) 48, 342, 451
Bsp19I CCATGG 1 cut(s) 283
BspACI CCGC 1 cut(s) 364
BspANI GGCC 2 cut(s) 303, 406
BspLI GGNNCC 1 cut(s) 50
BspMI ACCTGC 1 cut(s) 208
BspPI GGATC 4 cut(s) 43, 56, 337, 459
BssECI CCNNGG 2 cut(s) 283, 346
BssMI GATC 3 cut(s) 48, 342, 451
BssNAI GTATAC 1 cut(s) 168
BssSI CACGAG 1 cut(s) 396
BssT1I CCWWGG 1 cut(s) 283
Bst1107I GTATAC 1 cut(s) 168
Bst2BI CACGAG 1 cut(s) 396
Bst2UI CCWGG 1 cut(s) 348
Bst4CI ACNGT 2 cut(s) 151, 212
BstDEI CTNAG 1 cut(s) 53
BstDSI CCRYGG 1 cut(s) 283
BstKTI GATC 3 cut(s) 51, 345, 454
BstMAI GTCTC 1 cut(s) 423
BstMBI GATC 3 cut(s) 48, 342, 451
BstMWI GCNNNNNNNGC 1 cut(s) 92
BstNI CCWGG 1 cut(s) 348
BstNSI RCATGY 1 cut(s) 280
BstSCI CCNGG 1 cut(s) 346
BstX2I RGATCY 1 cut(s) 48
BstYI RGATCY 1 cut(s) 48
BstZ17I GTATAC 1 cut(s) 168
BsuRI GGCC 2 cut(s) 303, 406
BtgI CCRYGG 1 cut(s) 283
BveI ACCTGC 1 cut(s) 208
Csp6I GTAC 1 cut(s) 280
CviAII CATG 3 cut(s) 277, 284, 375
CviJI RGCY 3 cut(s) 57, 303, 406
CviKI_1 RGCY 3 cut(s) 57, 303, 406
CviQI GTAC 1 cut(s) 280
DdeI CTNAG 1 cut(s) 53
DpnI GATC 3 cut(s) 50, 344, 453
DpnII GATC 3 cut(s) 48, 342, 451
EaeI YGGCCR 1 cut(s) 301
Eco130I CCWWGG 1 cut(s) 283
EcoRII CCWGG 1 cut(s) 346
EcoT14I CCWWGG 1 cut(s) 283
ErhI CCWWGG 1 cut(s) 283
FaeI CATG 3 cut(s) 280, 287, 378
FatI CATG 3 cut(s) 276, 283, 374
FblI GTMKAC 1 cut(s) 167
FspBI CTAG 1 cut(s) 195
GsaI CCCAGC 1 cut(s) 183
HaeIII GGCC 2 cut(s) 303, 406
Hin1II CATG 3 cut(s) 280, 287, 378
HindIII AAGCTT 1 cut(s) 55
HphI GGTGA 1 cut(s) 19
Hpy166II GTNNAC 1 cut(s) 168
Hpy188I TCNGA 1 cut(s) 451
Hpy188III TCNNGA 2 cut(s) 215, 398
Hpy8I GTNNAC 1 cut(s) 168
HpyCH4III ACNGT 2 cut(s) 151, 212
HpyCH4V TGCA 4 cut(s) 86, 203, 324, 385
HpyF10VI GCNNNNNNNGC 1 cut(s) 92
HpyF3I CTNAG 1 cut(s) 53
Hsp92II CATG 3 cut(s) 280, 287, 378
Kzo9I GATC 3 cut(s) 48, 342, 451
LpnPI CCDG 7 cut(s) 165, 191, 200, 213, 314, 333, 360
LweI GCATC 2 cut(s) 60, 380
MaeI CTAG 1 cut(s) 195
MaeIII GTNAC 3 cut(s) 7, 145, 424
MalI GATC 3 cut(s) 50, 344, 453
MboI GATC 3 cut(s) 48, 342, 451
MflI RGATCY 1 cut(s) 48
MluCI AATT 4 cut(s) 173, 295, 351, 457
MnlI CCTC 5 cut(s) 27, 128, 281, 396, 405
MseI TTAA 1 cut(s) 460
MslI CAYNNNNRTG 1 cut(s) 376
MspR9I CCNGG 1 cut(s) 348
Mva1269I GAATGC 1 cut(s) 95
MvaI CCWGG 1 cut(s) 348
MwoI GCNNNNNNNGC 1 cut(s) 92
NcoI CCATGG 1 cut(s) 283
NdeII GATC 3 cut(s) 48, 342, 451
NlaIII CATG 3 cut(s) 280, 287, 378
NlaIV GGNNCC 1 cut(s) 50
NmuCI GTSAC 3 cut(s) 7, 145, 424
NspI RCATGY 1 cut(s) 280
PciI ACATGT 1 cut(s) 276
PctI GAATGC 1 cut(s) 95
PscI ACATGT 1 cut(s) 276
Psp6I CCWGG 1 cut(s) 346
PspFI CCCAGC 1 cut(s) 179
PspGI CCWGG 1 cut(s) 346
PspN4I GGNNCC 1 cut(s) 50
PsuI RGATCY 1 cut(s) 48
RsaI GTAC 1 cut(s) 281
RsaNI GTAC 1 cut(s) 280
RseI CAYNNNNRTG 1 cut(s) 376
SaqAI TTAA 1 cut(s) 460
Sau3AI GATC 3 cut(s) 48, 342, 451
ScrFI CCNGG 1 cut(s) 348
SetI ASST 4 cut(s) 59, 202, 242, 397
SfaNI GCATC 2 cut(s) 60, 380
SmiMI CAYNNNNRTG 1 cut(s) 376
Sse9I AATT 4 cut(s) 173, 295, 351, 457
SsiI CCGC 1 cut(s) 364
SspMI CTAG 1 cut(s) 195
StyD4I CCNGG 1 cut(s) 346
StyI CCWWGG 1 cut(s) 283
TaaI ACNGT 2 cut(s) 151, 212
TasI AATT 4 cut(s) 173, 295, 351, 457
Tru1I TTAA 1 cut(s) 460
Tru9I TTAA 1 cut(s) 460
TseFI GTSAC 3 cut(s) 7, 145, 424
Tsp45I GTSAC 3 cut(s) 7, 145, 424
TspDTI ATGAA 1 cut(s) 405
XapI RAATTY 2 cut(s) 173, 295
XceI RCATGY 1 cut(s) 280
XmiI GTMKAC 1 cut(s) 167
XspI CTAG 1 cut(s) 195
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.