Rh5CG208600

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0013715
Physical Location & Seq
Reverse (-)
21023333 .. 21025445
2113 bp
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UTR
Exon/CDS
Intron
Rh5CG208600.1

Sequence Viewer

Length: 537 bp
ATGGATTTCGTGACAATGCTGTGTAACTCTCTATTTTTGAGAATCAGGAAATGGGACTGTGCCTTGGTTGTATCTAGGCGGACCAATCTACTGTTGCAATCAGAGAAACTTTTGGGAAGTTTTATGATGGGCTTGAACCTGATGTTTGTTAGTGTGGAGCTTGATAAAAAATTGTCTAGTCAAAATGCCTGGTATACAGAATTTGCTGGGGCAGTTCCTACTAGACCTGCACAGGGCAGTCAGGACTTGGCATTTTCAGTTTCAAGGTTTATACAAAATGGTGGTTCTTTTGCCAACTACTACATGTACCATGGAGGAACGAATTTTGGCCGAACTGCTAATATGGTATGCAATCTGGCAGAAAGAGTGATCCCAGGAATTGAGTTGGTGCGGTTAGCATCATGGTGTTTGCAGTATGAACCTTGTGAGAGGCCAAATGCCAAGTCTCTTGTGACTGCCCTTGTTCTTCTTCAAAAGGAAACTTCGTTGAAGCGTAAAGATATTATATCAGCTTTTGTTTCATGTATAGGTAGTTAG

Protein Analysis

178

Amino Acids

19.94

Weight (kDa)

9.13

Isoelectric Point (pI)

39.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_35 PF01301 61 - 114 2.6e-10 Glycosyl hydrolases family 35
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 235
AccI GTMKAC 1 cut(s) 194
AciI CCGC 2 cut(s) 79, 391
AclWI GGATC 1 cut(s) 364
AcoI YGGCCR 1 cut(s) 328
AcsI RAATTY 2 cut(s) 200, 322
AfaI GTAC 1 cut(s) 308
AfiI CCNNNNNNNGG 1 cut(s) 233
AflIII ACRYGT 1 cut(s) 303
AgsI TTSAA 4 cut(s) 136, 264, 473, 490
AjnI CCWGG 2 cut(s) 188, 373
AluBI AGCT 2 cut(s) 160, 512
AluI AGCT 2 cut(s) 160, 512
Alw26I GTCTC 1 cut(s) 450
AlwI GGATC 1 cut(s) 364
AoxI GGCC 2 cut(s) 328, 431
ApoI RAATTY 2 cut(s) 200, 322
ArsI GACNNNNNNTTYG 2 cut(s) 428, 460
AspS9I GGNCC 1 cut(s) 81
AvaII GGWCC 1 cut(s) 81
BccI CCATC 1 cut(s) 121
BciT130I CCWGG 2 cut(s) 190, 375
BcoDI GTCTC 1 cut(s) 450
BfaI CTAG 3 cut(s) 75, 177, 222
BfuAI ACCTGC 1 cut(s) 235
Bme1390I CCNGG 2 cut(s) 190, 375
Bme18I GGWCC 1 cut(s) 81
BmgT120I GGNCC 1 cut(s) 81
BmrFI CCNGG 2 cut(s) 190, 375
BmsI GCATC 1 cut(s) 407
BsaJI CCNNGG 3 cut(s) 63, 310, 373
Bsc4I CCNNNNNNNGG 1 cut(s) 233
BseBI CCWGG 2 cut(s) 190, 375
BseDI CCNNGG 3 cut(s) 63, 310, 373
BseLI CCNNNNNNNGG 1 cut(s) 233
BseYI CCCAGC 1 cut(s) 206
BsgI GTGCAG 1 cut(s) 213
BshFI GGCC 2 cut(s) 330, 433
BslFI GGGAC 1 cut(s) 68
BslI CCNNNNNNNGG 1 cut(s) 233
BsmAI GTCTC 1 cut(s) 450
BsmFI GGGAC 1 cut(s) 68
BsnI GGCC 2 cut(s) 330, 433
Bsp143I GATC 1 cut(s) 369
Bsp19I CCATGG 1 cut(s) 310
BspACI CCGC 2 cut(s) 79, 391
BspANI GGCC 2 cut(s) 330, 433
BspMI ACCTGC 1 cut(s) 235
BspPI GGATC 1 cut(s) 364
BssECI CCNNGG 3 cut(s) 63, 310, 373
BssMI GATC 1 cut(s) 369
BssNAI GTATAC 1 cut(s) 195
BssT1I CCWWGG 2 cut(s) 63, 310
Bst1107I GTATAC 1 cut(s) 195
Bst2UI CCWGG 2 cut(s) 190, 375
Bst4CI ACNGT 2 cut(s) 59, 93
BstDSI CCRYGG 1 cut(s) 310
BstKTI GATC 1 cut(s) 372
BstMAI GTCTC 1 cut(s) 450
BstMBI GATC 1 cut(s) 369
BstNI CCWGG 2 cut(s) 190, 375
BstNSI RCATGY 1 cut(s) 307
BstSCI CCNGG 2 cut(s) 188, 373
BstZ17I GTATAC 1 cut(s) 195
BsuRI GGCC 2 cut(s) 330, 433
BtgI CCRYGG 1 cut(s) 310
BveI ACCTGC 1 cut(s) 235
Cfr13I GGNCC 1 cut(s) 81
Csp6I GTAC 1 cut(s) 307
CviAII CATG 4 cut(s) 304, 311, 402, 522
CviJI RGCY 5 cut(s) 132, 160, 330, 433, 512
CviKI_1 RGCY 5 cut(s) 132, 160, 330, 433, 512
CviQI GTAC 1 cut(s) 307
DpnI GATC 1 cut(s) 371
DpnII GATC 1 cut(s) 369
EaeI YGGCCR 1 cut(s) 328
EciI GGCGGA 1 cut(s) 94
Eco130I CCWWGG 2 cut(s) 63, 310
Eco47I GGWCC 1 cut(s) 81
EcoRII CCWGG 2 cut(s) 188, 373
EcoT14I CCWWGG 2 cut(s) 63, 310
ErhI CCWWGG 2 cut(s) 63, 310
FaeI CATG 4 cut(s) 307, 314, 405, 525
FaqI GGGAC 1 cut(s) 68
FatI CATG 4 cut(s) 303, 310, 401, 521
FblI GTMKAC 1 cut(s) 194
FspBI CTAG 3 cut(s) 75, 177, 222
GsaI CCCAGC 1 cut(s) 210
HaeIII GGCC 2 cut(s) 330, 433
Hin1II CATG 4 cut(s) 307, 314, 405, 525
HinfI GANTC 1 cut(s) 42
Hpy166II GTNNAC 1 cut(s) 195
Hpy188I TCNGA 1 cut(s) 103
Hpy188III TCNNGA 3 cut(s) 10, 46, 242
Hpy8I GTNNAC 1 cut(s) 195
HpyCH4III ACNGT 2 cut(s) 59, 93
HpyCH4V TGCA 4 cut(s) 97, 230, 351, 412
Hsp92II CATG 4 cut(s) 307, 314, 405, 525
Kzo9I GATC 1 cut(s) 369
LmnI GCTCC 1 cut(s) 157
LweI GCATC 1 cut(s) 407
MaeI CTAG 3 cut(s) 75, 177, 222
MaeIII GTNAC 3 cut(s) 10, 23, 451
MalI GATC 1 cut(s) 371
MboI GATC 1 cut(s) 369
MboII GAAGA 2 cut(s) 458, 461
MluCI AATT 4 cut(s) 170, 200, 322, 378
MnlI CCTC 2 cut(s) 308, 423
MslI CAYNNNNRTG 1 cut(s) 403
MspR9I CCNGG 2 cut(s) 190, 375
MvaI CCWGG 2 cut(s) 190, 375
NcoI CCATGG 1 cut(s) 310
NdeII GATC 1 cut(s) 369
NlaIII CATG 4 cut(s) 307, 314, 405, 525
NmuCI GTSAC 2 cut(s) 10, 451
NspI RCATGY 1 cut(s) 307
PciI ACATGT 1 cut(s) 303
PfeI GAWTC 1 cut(s) 42
PscI ACATGT 1 cut(s) 303
Psp6I CCWGG 2 cut(s) 188, 373
PspFI CCCAGC 1 cut(s) 206
PspGI CCWGG 2 cut(s) 188, 373
PspPI GGNCC 1 cut(s) 81
RsaI GTAC 1 cut(s) 308
RsaNI GTAC 1 cut(s) 307
RseI CAYNNNNRTG 1 cut(s) 403
Sau3AI GATC 1 cut(s) 369
Sau96I GGNCC 1 cut(s) 81
ScrFI CCNGG 2 cut(s) 190, 375
SetI ASST 7 cut(s) 141, 162, 229, 269, 424, 514, 532
SfaNI GCATC 1 cut(s) 407
SinI GGWCC 1 cut(s) 81
SmiMI CAYNNNNRTG 1 cut(s) 403
Sse9I AATT 4 cut(s) 170, 200, 322, 378
SsiI CCGC 2 cut(s) 79, 391
SspMI CTAG 3 cut(s) 75, 177, 222
StyD4I CCNGG 2 cut(s) 188, 373
StyI CCWWGG 2 cut(s) 63, 310
TaaI ACNGT 2 cut(s) 59, 93
TasI AATT 4 cut(s) 170, 200, 322, 378
TfiI GAWTC 1 cut(s) 42
TseFI GTSAC 2 cut(s) 10, 451
Tsp45I GTSAC 2 cut(s) 10, 451
TspDTI ATGAA 2 cut(s) 432, 510
VpaK11BI GGWCC 1 cut(s) 81
XapI RAATTY 2 cut(s) 200, 322
XceI RCATGY 1 cut(s) 307
XmiI GTMKAC 1 cut(s) 194
XspI CTAG 3 cut(s) 75, 177, 222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.