Rh2DG289000

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0017467
Physical Location & Seq
Forward (+)
33878395 .. 33883489
5095 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG289000.1

Sequence Viewer

Length: 552 bp
ATGGGCGATGTCGTTGGTGGTGGTATCACTGTCGGGGATGATGATGATGGTTGGTGGCAGAAGAGTGTTTGGGTTTCCCCAGATCGTTCTTTGTTTGCGCATATTGGTTTTGGTGACTGGAGTGGTGGTCCTAGTTTCGGAGGTAGGTTTTGTGGTGAGCGCGACGACCAAAGTGGCAGTAGAGGTGGCGACCAAGTAGGCGGTGGCATTCATGGTGGCGGCGCTAGTAGATTTGGCGATGAGGGTGAGGCTGAGACTCCTTTTAGGCTCAGTGGAGCTTTGGCCTTGAGGGGTTTGGAAGTGAAGAAATCTGGAGTTCGTATAGTGTACTATCAAGATGAGGAAGAGAGTAAGGTTAACAATAATTCTCAGGAGCCTTTCACATTTTCTGACCCATCTCCTAGTGAGGCAGCAACTGTATCAGGTGCGACCACCAAGCGAGGTCGTGAACACTTTGAGTTTGGGTTTGGGTTTGACAACAAAGCTGATGGTTTGCTAGGACAACCCAAGAAAAAAGTTTCTTTCAATCTTAATTCATGTTTAATAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

183

Amino Acids

19.47

Weight (kDa)

4.85

Isoelectric Point (pI)

46.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 99
AccII CGCG 1 cut(s) 162
AciI CCGC 2 cut(s) 201, 219
AfaI GTAC 1 cut(s) 329
AfiI CCNNNNNNNGG 1 cut(s) 137
AgsI TTSAA 1 cut(s) 526
AluBI AGCT 2 cut(s) 278, 485
AluI AGCT 2 cut(s) 278, 485
Alw26I GTCTC 1 cut(s) 248
AlwNI CAGNNNCTG 1 cut(s) 416
AoxI GGCC 1 cut(s) 282
ApeKI GCWGC 1 cut(s) 410
AspLEI GCGC 3 cut(s) 100, 162, 224
AspS9I GGNCC 1 cut(s) 128
AsuHPI GGTGA 3 cut(s) 125, 167, 257
AvaII GGWCC 1 cut(s) 128
BbvI GCAGC 1 cut(s) 422
BccI CCATC 3 cut(s) 41, 403, 482
BcoDI GTCTC 1 cut(s) 248
BfaI CTAG 4 cut(s) 132, 225, 402, 497
BfoI RGCGCY 1 cut(s) 225
BisI GCNGC 2 cut(s) 220, 411
BlsI GCNGC 2 cut(s) 221, 412
Bme18I GGWCC 1 cut(s) 128
BmgT120I GGNCC 1 cut(s) 128
BmiI GGNNCC 1 cut(s) 375
BpmI CTGGAG 2 cut(s) 139, 333
BpuEI CTTGAG 1 cut(s) 307
BsaXI ACNNNNNCTCC 2 cut(s) 112, 142
Bsc4I CCNNNNNNNGG 1 cut(s) 137
Bse1I ACTGG 1 cut(s) 122
BseGI GGATG 1 cut(s) 43
BseLI CCNNNNNNNGG 1 cut(s) 137
BseMII CTCAG 3 cut(s) 243, 283, 383
BseNI ACTGG 1 cut(s) 122
BseXI GCAGC 1 cut(s) 422
Bsh1236I CGCG 1 cut(s) 162
BshFI GGCC 1 cut(s) 284
BslI CCNNNNNNNGG 1 cut(s) 137
BsmAI GTCTC 1 cut(s) 248
BsmI GAATGC 1 cut(s) 207
BsnI GGCC 1 cut(s) 284
Bsp143I GATC 1 cut(s) 82
BspACI CCGC 2 cut(s) 201, 219
BspANI GGCC 1 cut(s) 284
BspCNI CTCAG 3 cut(s) 244, 282, 382
BspFNI CGCG 1 cut(s) 162
BspLI GGNNCC 1 cut(s) 375
BsrI ACTGG 1 cut(s) 122
BssMI GATC 1 cut(s) 82
Bst4CI ACNGT 2 cut(s) 31, 418
Bst6I CTCTTC 2 cut(s) 56, 339
BstDEI CTNAG 3 cut(s) 252, 269, 369
BstF5I GGATG 1 cut(s) 43
BstFNI CGCG 1 cut(s) 162
BstH2I RGCGCY 1 cut(s) 225
BstHHI GCGC 3 cut(s) 100, 162, 224
BstKTI GATC 1 cut(s) 85
BstMAI GTCTC 1 cut(s) 248
BstMBI GATC 1 cut(s) 82
BstUI CGCG 1 cut(s) 162
BstV1I GCAGC 1 cut(s) 422
BsuRI GGCC 1 cut(s) 284
BtgZI GCGATG 2 cut(s) 21, 252
BtsCI GGATG 1 cut(s) 43
BtsIMutI CAGTG 2 cut(s) 27, 277
CaiI CAGNNNCTG 1 cut(s) 416
CfoI GCGC 3 cut(s) 100, 162, 224
Cfr13I GGNCC 1 cut(s) 128
Csp6I GTAC 1 cut(s) 328
CviAII CATG 2 cut(s) 212, 537
CviJI RGCY 6 cut(s) 251, 268, 278, 284, 376, 485
CviKI_1 RGCY 6 cut(s) 251, 268, 278, 284, 376, 485
CviQI GTAC 1 cut(s) 328
DdeI CTNAG 3 cut(s) 252, 269, 369
DpnI GATC 1 cut(s) 84
DpnII GATC 1 cut(s) 82
Eam1104I CTCTTC 2 cut(s) 56, 339
EarI CTCTTC 2 cut(s) 56, 339
Eco47I GGWCC 1 cut(s) 128
FaeI CATG 2 cut(s) 215, 540
FaiI YATR 4 cut(s) 102, 213, 323, 538
FatI CATG 2 cut(s) 211, 536
Fnu4HI GCNGC 2 cut(s) 220, 411
FokI GGATG 1 cut(s) 50
Fsp4HI GCNGC 2 cut(s) 220, 411
FspBI CTAG 4 cut(s) 132, 225, 402, 497
FspI TGCGCA 1 cut(s) 99
GlaI GCGC 3 cut(s) 99, 161, 223
GluI GCNGC 2 cut(s) 220, 411
GsuI CTGGAG 2 cut(s) 139, 333
HaeII RGCGCY 1 cut(s) 225
HaeIII GGCC 1 cut(s) 284
HhaI GCGC 3 cut(s) 100, 162, 224
Hin1II CATG 2 cut(s) 215, 540
Hin6I GCGC 3 cut(s) 98, 160, 222
HinP1I GCGC 3 cut(s) 98, 160, 222
HincII GTYRAC 1 cut(s) 358
HindII GTYRAC 1 cut(s) 358
HinfI GANTC 1 cut(s) 256
HpaI GTTAAC 1 cut(s) 358
HphI GGTGA 3 cut(s) 125, 167, 257
Hpy166II GTNNAC 3 cut(s) 328, 358, 449
Hpy188I TCNGA 2 cut(s) 140, 391
Hpy188III TCNNGA 4 cut(s) 312, 335, 371, 446
Hpy8I GTNNAC 3 cut(s) 328, 358, 449
Hpy99I CGWCG 1 cut(s) 167
HpyCH4III ACNGT 2 cut(s) 31, 418
HpyF3I CTNAG 3 cut(s) 252, 269, 369
Hsp92II CATG 2 cut(s) 215, 540
HspAI GCGC 3 cut(s) 98, 160, 222
KspAI GTTAAC 1 cut(s) 358
Kzo9I GATC 1 cut(s) 82
LmnI GCTCC 2 cut(s) 275, 373
LpnPI CCDG 5 cut(s) 93, 103, 297, 356, 408
Lsp1109I GCAGC 1 cut(s) 422
MaeI CTAG 4 cut(s) 132, 225, 402, 497
MaeIII GTNAC 1 cut(s) 113
MalI GATC 1 cut(s) 84
MboI GATC 1 cut(s) 82
MboII GAAGA 3 cut(s) 73, 316, 356
MluCI AATT 2 cut(s) 364, 532
MlyI GAGTC 1 cut(s) 250
MnlI CCTC 8 cut(s) 134, 176, 235, 241, 282, 334, 400, 434
MseI TTAA 3 cut(s) 357, 531, 542
Mva1269I GAATGC 1 cut(s) 207
MvnI CGCG 1 cut(s) 162
NdeII GATC 1 cut(s) 82
NlaIII CATG 2 cut(s) 215, 540
NlaIV GGNNCC 1 cut(s) 375
NmuCI GTSAC 1 cut(s) 113
NsbI TGCGCA 1 cut(s) 99
PctI GAATGC 1 cut(s) 207
PkrI GCNGC 2 cut(s) 221, 412
PleI GAGTC 1 cut(s) 250
PpsI GAGTC 1 cut(s) 250
PspN4I GGNNCC 1 cut(s) 375
PspPI GGNCC 1 cut(s) 128
PstNI CAGNNNCTG 1 cut(s) 416
RsaI GTAC 1 cut(s) 329
RsaNI GTAC 1 cut(s) 328
SaqAI TTAA 3 cut(s) 357, 531, 542
SatI GCNGC 2 cut(s) 220, 411
Sau3AI GATC 1 cut(s) 82
Sau96I GGNCC 1 cut(s) 128
SchI GAGTC 1 cut(s) 250
SetI ASST 8 cut(s) 145, 149, 187, 280, 357, 427, 445, 487
SinI GGWCC 1 cut(s) 128
SmlI CTYRAG 1 cut(s) 286
SmoI CTYRAG 1 cut(s) 286
Sse9I AATT 2 cut(s) 364, 532
SsiI CCGC 2 cut(s) 201, 219
SspMI CTAG 4 cut(s) 132, 225, 402, 497
TaaI ACNGT 2 cut(s) 31, 418
TasI AATT 2 cut(s) 364, 532
TatI WGTACW 1 cut(s) 327
TauI GCSGC 1 cut(s) 222
Tru1I TTAA 3 cut(s) 357, 531, 542
Tru9I TTAA 3 cut(s) 357, 531, 542
TscAI CASTG 2 cut(s) 34, 277
TseFI GTSAC 1 cut(s) 113
TseI GCWGC 1 cut(s) 410
Tsp45I GTSAC 1 cut(s) 113
TspDTI ATGAA 2 cut(s) 200, 525
TspRI CASTG 2 cut(s) 34, 277
VpaK11BI GGWCC 1 cut(s) 128
XcmI CCANNNNNNNNNTGG 1 cut(s) 200
XspI CTAG 4 cut(s) 132, 225, 402, 497
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.