Rh2DG357200

Belongs to the cytochrome P450 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0004522
Physical Location & Seq
Reverse (-)
50298540 .. 50302006
3467 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG357200.1

Sequence Viewer

Length: 1473 bp
ATGCTGAGTGCAATATCAAAAGAAGAGTTCCTCTTCCTTGTTAAGAAGTATTATGATGTTCTCATTGTTGGACTACTGTTTTCTATCGGAGTTGCATTTTTCGTCTCTAAATTAGCATGGAGAAAGGTCACAGGTTCCAGAGGTCACATCCCTGGCCGGTTGGGGTTACCCTTTATAGGTGAAACCATTTCTTTCATGTCAGCAACCAATAGCACCAGAGGATGCTATGATTTTATCAGATTCCGGCGAATATGGTATGGGAAGTGGTTCAAGACGAGAATTTTTGGCAAGATTCATGTGTTTGTTCCGAGTACAGAGGGTGCAAAAATGATATTTTCTAATGATTTTGCTAAGTTCAACAAAGGATATGTGAAATCAATGGCAGATTGTATTGGAGATAAAAGCTTGCTCTGTGTACCACATGAGAACCATAAAAGAATAAGGCGCTTATTATCAGATCCTTTCTCCATGAATTCTTTATCTTCGTTCATTGAGAAATTCGATAGAATGCTGTGTCAGAAGCTGAAGAAACTAGAACAAGGTGGGAAAAGCTTTTCGGTGCTTGACTTCAGTATGAGGATGACATTTGATGCGATGTGCTGCATGCTAATGAGTGTCATGGATGAGCCCTTGCTAAGGCAGCTTGAGAAAGACTGCACTGCAGTCTCGGATGGAATCTTATCTGTTCCTGTCATGATTCCAGGCACTAGATATTACAAAGGCATCAAGGCACGCAGACGTCTCATGGAAACCTTTAAAGACATGATTGCCAGAAGAAGGAGTGGGAAGGAGTTGCCAGAACCAGAAGATTTCCTACAGTCCATGTTGCGAAGAGACTCATACCCTCCCAATGAAAAGCTGCAAGACTCAGAAATCATGGACAACCTAATGACACTTATAATTGCAGGGCAGACGACCACTTCAGCTGCAATGATGTGGAGTGTCAAGTTTCTGGATGAGAACCAACAAGTCCAAGACATTCTTAGGGAGGAACAATTGTCTATAGCCACATCCAAGCCCGAAGGAGCATCAATTACCCTCGAAGATATTAATAATATGTCCTACTGTTGGAAGGTTGTTAAAGAAACAGTAAGGATGTCAACTGTGGTTATGTGGTATCCTCGAGTTGCACTCAGTGACTGTACTATAGAAGGTTTTGAGATAAAGAAAGGGTGGCATGTGAACATTGACGCGACATGCATACACAACGACCCAGATTTGTACCCAGAACCTTCCAAATTCAACCCTTCTAGATTTGATGAAATGCAAAAGCCATATAGTTTCATACCATTTGGGTCAGGACCCAGGACATGCTTGGGAATCAATATGGCCAAGATGACAATGATGGTCTTTCTTCACCGTCTCACTACTTCATACAGGTGGACAGTCGACGATCTTGATACCAGTCTAGAAAAGAAGGCACATATTCCTAGACTAAGAAGTGGTTGTCCCATTACCTTGACGGCCTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00830 ko01100 map00830 map01100
Pfam Domains
Protein Families
p450

Protein Analysis

490

Amino Acids

56.3

Weight (kDa)

9.14

Isoelectric Point (pI)

49.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 55 - 468 3.8e-67 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 899, 1471
AatII GACGTC 1 cut(s) 742
AccI GTMKAC 1 cut(s) 1389
AccII CGCG 1 cut(s) 1193
AclWI GGATC 1 cut(s) 452
AcoI YGGCCR 2 cut(s) 154, 1329
AcsI RAATTY 4 cut(s) 279, 472, 497, 1238
AcuI CTGAAG 3 cut(s) 545, 553, 906
AcyI GRCGYC 1 cut(s) 739
AdeI CACNNNGTG 1 cut(s) 1136
AfaI GTAC 4 cut(s) 313, 417, 1144, 1223
AfiI CCNNNNNNNGG 4 cut(s) 176, 636, 777, 1068
AgsI TTSAA 3 cut(s) 271, 358, 1243
AjnI CCWGG 3 cut(s) 151, 700, 1304
AluBI AGCT 6 cut(s) 405, 523, 552, 643, 859, 926
AluI AGCT 6 cut(s) 405, 523, 552, 643, 859, 926
Alw26I GTCTC 5 cut(s) 109, 670, 746, 828, 1367
AlwI GGATC 1 cut(s) 452
AlwNI CAGNNNCTG 2 cut(s) 523, 1140
Ama87I CYCGRG 1 cut(s) 1122
AoxI GGCC 3 cut(s) 154, 1329, 1464
ApeKI GCWGC 4 cut(s) 600, 640, 859, 926
ApoI RAATTY 4 cut(s) 279, 472, 497, 1238
AseI ATTAAT 1 cut(s) 1050
Asp700I GAANNNNTTC 1 cut(s) 266
AspLEI GCGC 1 cut(s) 447
AspS9I GGNCC 1 cut(s) 1301
AsuHPI GGTGA 2 cut(s) 191, 1349
AvaI CYCGRG 1 cut(s) 1122
AvaII GGWCC 1 cut(s) 1301
BalI TGGCCA 1 cut(s) 1331
BanII GRGCYC 1 cut(s) 630
BarI GAAGNNNNNNTAC 2 cut(s) 798, 830
BbvI GCAGC 4 cut(s) 587, 652, 846, 913
BccI CCATC 2 cut(s) 665, 1339
BciT130I CCWGG 3 cut(s) 153, 702, 1306
BciVI GTATCC 1 cut(s) 1128
BcoDI GTCTC 5 cut(s) 109, 670, 746, 828, 1367
BfaI CTAG 5 cut(s) 533, 708, 1251, 1409, 1431
BfmI CTRYAG 4 cut(s) 660, 815, 1002, 1146
BfoI RGCGCY 1 cut(s) 448
BfuI GTATCC 1 cut(s) 1128
BisI GCNGC 4 cut(s) 601, 641, 860, 927
BlsI GCNGC 4 cut(s) 602, 642, 861, 928
Bme1390I CCNGG 3 cut(s) 153, 702, 1306
Bme18I GGWCC 1 cut(s) 1301
BmeT110I CYCGRG 1 cut(s) 1122
BmgT120I GGNCC 1 cut(s) 1301
BmiI GGNNCC 2 cut(s) 136, 1303
BmrFI CCNGG 3 cut(s) 153, 702, 1306
BmsI GCATC 4 cut(s) 212, 580, 732, 1037
BplI GAGNNNNNCTC 2 cut(s) 1116, 1148
Bpu10I CCTNAGC 1 cut(s) 635
BpuEI CTTGAG 1 cut(s) 665
BsaHI GRCGYC 1 cut(s) 739
BsaJI CCNNGG 2 cut(s) 151, 1304
Bsc4I CCNNNNNNNGG 4 cut(s) 176, 636, 777, 1068
Bse118I RCCGGY 1 cut(s) 156
Bse1I ACTGG 1 cut(s) 1404
Bse3DI GCAATG 1 cut(s) 936
BseBI CCWGG 3 cut(s) 153, 702, 1306
BseDI CCNNGG 2 cut(s) 151, 1304
BseGI GGATG 8 cut(s) 147, 227, 585, 628, 676, 961, 1010, 1101
BseLI CCNNNNNNNGG 4 cut(s) 176, 636, 777, 1068
BseMI GCAATG 1 cut(s) 936
BseMII CTCAG 2 cut(s) 882, 1147
BseNI ACTGG 1 cut(s) 1404
BseXI GCAGC 4 cut(s) 587, 652, 846, 913
BsgI GTGCAG 1 cut(s) 640
Bsh1236I CGCG 1 cut(s) 1193
BshFI GGCC 3 cut(s) 156, 1331, 1466
BsiHKCI CYCGRG 1 cut(s) 1122
BsiSI CCGG 2 cut(s) 157, 244
BslFI GGGAC 1 cut(s) 1434
BslI CCNNNNNNNGG 4 cut(s) 176, 636, 777, 1068
BsmAI GTCTC 5 cut(s) 109, 670, 746, 828, 1367
BsmBI CGTCTC 3 cut(s) 109, 746, 1367
BsmFI GGGAC 1 cut(s) 1434
BsmI GAATGC 1 cut(s) 513
BsnI GGCC 3 cut(s) 156, 1331, 1466
BsoBI CYCGRG 1 cut(s) 1122
Bsp1286I GDGCHC 1 cut(s) 630
Bsp143I GATC 2 cut(s) 457, 1393
BspANI GGCC 3 cut(s) 156, 1331, 1466
BspCNI CTCAG 2 cut(s) 881, 1146
BspFNI CGCG 1 cut(s) 1193
BspHI TCATGA 1 cut(s) 693
BspLI GGNNCC 2 cut(s) 136, 1303
BspMAI CTGCAG 1 cut(s) 664
BspPI GGATC 1 cut(s) 452
BsrDI GCAATG 1 cut(s) 936
BsrFI RCCGGY 1 cut(s) 156
BsrI ACTGG 1 cut(s) 1404
BssAI RCCGGY 1 cut(s) 156
BssECI CCNNGG 2 cut(s) 151, 1304
BssMI GATC 2 cut(s) 457, 1393
BssNI GRCGYC 1 cut(s) 739
Bst2UI CCWGG 3 cut(s) 153, 702, 1306
Bst4CI ACNGT 8 cut(s) 78, 819, 1067, 1090, 1105, 1142, 1361, 1387
Bst6I CTCTTC 3 cut(s) 18, 38, 826
BstACI GRCGYC 1 cut(s) 739
BstC8I GCNNGC 3 cut(s) 407, 605, 733
BstDEI CTNAG 7 cut(s) 5, 351, 635, 868, 983, 1133, 1436
BstEII GGTNACC 1 cut(s) 165
BstENI CCTNNNNNAGG 1 cut(s) 634
BstF5I GGATG 8 cut(s) 147, 227, 585, 628, 676, 961, 1010, 1101
BstFNI CGCG 1 cut(s) 1193
BstH2I RGCGCY 1 cut(s) 448
BstHHI GCGC 1 cut(s) 447
BstKTI GATC 2 cut(s) 460, 1396
BstMAI GTCTC 5 cut(s) 109, 670, 746, 828, 1367
BstMBI GATC 2 cut(s) 457, 1393
BstMWI GCNNNNNNNGC 1 cut(s) 640
BstNI CCWGG 3 cut(s) 153, 702, 1306
BstNSI RCATGY 4 cut(s) 607, 1181, 1200, 1314
BstPI GGTNACC 1 cut(s) 165
BstSCI CCNGG 3 cut(s) 151, 700, 1304
BstSFI CTRYAG 4 cut(s) 660, 815, 1002, 1146
BstUI CGCG 1 cut(s) 1193
BstV1I GCAGC 4 cut(s) 587, 652, 846, 913
BstX2I RGATCY 1 cut(s) 457
BstYI RGATCY 1 cut(s) 457
BsuI GTATCC 1 cut(s) 1128
BsuRI GGCC 3 cut(s) 156, 1331, 1466
BtgZI GCGATG 1 cut(s) 608
BtsCI GGATG 8 cut(s) 147, 227, 585, 628, 676, 961, 1010, 1101
BtsI GCAGTG 1 cut(s) 657
BtsIMutI CAGTG 2 cut(s) 657, 1141
Cac8I GCNNGC 3 cut(s) 407, 605, 733
CaiI CAGNNNCTG 2 cut(s) 523, 1140
CciI TCATGA 1 cut(s) 693
CfoI GCGC 1 cut(s) 447
Cfr10I RCCGGY 1 cut(s) 156
Cfr13I GGNCC 1 cut(s) 1301
CseI GACGC 1 cut(s) 1199
Csp6I GTAC 4 cut(s) 312, 416, 1143, 1222
CviQI GTAC 4 cut(s) 312, 416, 1143, 1222
DdeI CTNAG 7 cut(s) 5, 351, 635, 868, 983, 1133, 1436
DpnI GATC 2 cut(s) 459, 1395
DpnII GATC 2 cut(s) 457, 1393
DraI TTTAAA 1 cut(s) 757
DraIII CACNNNGTG 1 cut(s) 1136
EaeI YGGCCR 2 cut(s) 154, 1329
Eam1104I CTCTTC 3 cut(s) 18, 38, 826
EarI CTCTTC 3 cut(s) 18, 38, 826
Eco24I GRGCYC 1 cut(s) 630
Eco47I GGWCC 1 cut(s) 1301
Eco57I CTGAAG 3 cut(s) 545, 553, 906
Eco88I CYCGRG 1 cut(s) 1122
Eco91I GGTNACC 1 cut(s) 165
EcoNI CCTNNNNNAGG 1 cut(s) 634
EcoO109I RGGNCCY 1 cut(s) 1301
EcoO65I GGTNACC 1 cut(s) 165
EcoRI GAATTC 1 cut(s) 472
EcoRII CCWGG 3 cut(s) 151, 700, 1304
EcoT22I ATGCAT 1 cut(s) 1202
EcoT38I GRGCYC 1 cut(s) 630
Esp3I CGTCTC 3 cut(s) 109, 746, 1367
FalI AAGNNNNNCTT 2 cut(s) 966, 998
FaqI GGGAC 1 cut(s) 1434
FblI GTMKAC 1 cut(s) 1389
Fnu4HI GCNGC 4 cut(s) 601, 641, 860, 927
FokI GGATG 8 cut(s) 134, 234, 592, 635, 683, 968, 997, 1108
FriOI GRGCYC 1 cut(s) 630
Fsp4HI GCNGC 4 cut(s) 601, 641, 860, 927
FspBI CTAG 5 cut(s) 533, 708, 1251, 1409, 1431
GlaI GCGC 1 cut(s) 446
GluI GCNGC 4 cut(s) 601, 641, 860, 927
HaeII RGCGCY 1 cut(s) 448
HaeIII GGCC 3 cut(s) 156, 1331, 1466
HapII CCGG 2 cut(s) 157, 244
HgaI GACGC 1 cut(s) 1199
HhaI GCGC 1 cut(s) 447
Hin1I GRCGYC 1 cut(s) 739
Hin6I GCGC 1 cut(s) 445
HinP1I GCGC 1 cut(s) 445
HincII GTYRAC 2 cut(s) 1101, 1390
HindII GTYRAC 2 cut(s) 1101, 1390
HindIII AAGCTT 2 cut(s) 403, 550
HinfI GANTC 7 cut(s) 240, 292, 675, 697, 836, 866, 1320
HpaII CCGG 2 cut(s) 157, 244
HphI GGTGA 2 cut(s) 191, 1349
Hpy166II GTNNAC 5 cut(s) 416, 1101, 1183, 1383, 1390
Hpy188I TCNGA 7 cut(s) 89, 239, 309, 457, 519, 670, 871
Hpy188III TCNNGA 8 cut(s) 138, 271, 694, 953, 1251, 1299, 1397, 1409
Hpy8I GTNNAC 5 cut(s) 416, 1101, 1183, 1383, 1390
Hpy99I CGWCG 1 cut(s) 1394
HpyAV CCTTC 8 cut(s) 771, 781, 1016, 1066, 1145, 1242, 1257, 1411
HpyCH4III ACNGT 8 cut(s) 78, 819, 1067, 1090, 1105, 1142, 1361, 1387
HpyCH4IV ACGT 1 cut(s) 739
HpyF10VI GCNNNNNNNGC 1 cut(s) 640
HpyF3I CTNAG 7 cut(s) 5, 351, 635, 868, 983, 1133, 1436
HpySE526I ACGT 1 cut(s) 739
Hsp92I GRCGYC 1 cut(s) 739
HspAI GCGC 1 cut(s) 445
Kzo9I GATC 2 cut(s) 457, 1393
LmnI GCTCC 1 cut(s) 1025
Lsp1109I GCAGC 4 cut(s) 587, 652, 846, 913
LweI GCATC 4 cut(s) 212, 580, 732, 1037
MaeI CTAG 5 cut(s) 533, 708, 1251, 1409, 1431
MaeII ACGT 1 cut(s) 739
MaeIII GTNAC 4 cut(s) 127, 143, 165, 1136
MalI GATC 2 cut(s) 459, 1395
MboI GATC 2 cut(s) 457, 1393
MboII GAAGA 9 cut(s) 25, 35, 474, 538, 786, 818, 843, 1055, 1346
MfeI CAATTG 1 cut(s) 995
MflI RGATCY 1 cut(s) 457
MhlI GDGCHC 1 cut(s) 630
MlsI TGGCCA 1 cut(s) 1331
MluCI AATT 8 cut(s) 110, 279, 472, 497, 900, 995, 1032, 1238
MluNI TGGCCA 1 cut(s) 1331
MlyI GAGTC 2 cut(s) 830, 860
MmeI TCCRAC 2 cut(s) 49, 1049
MnlI CCTC 9 cut(s) 41, 134, 212, 310, 570, 855, 982, 1049, 1131
Mox20I TGGCCA 1 cut(s) 1331
Mph1103I ATGCAT 1 cut(s) 1202
MroXI GAANNNNTTC 1 cut(s) 266
MscI TGGCCA 1 cut(s) 1331
MseI TTAA 4 cut(s) 42, 756, 1050, 1080
MslI CAYNNNNRTG 2 cut(s) 608, 1378
Msp20I TGGCCA 1 cut(s) 1331
MspA1I CMGCKG 1 cut(s) 926
MspI CCGG 2 cut(s) 157, 244
MspR9I CCNGG 3 cut(s) 153, 702, 1306
MunI CAATTG 1 cut(s) 995
Mva1269I GAATGC 1 cut(s) 513
MvaI CCWGG 3 cut(s) 153, 702, 1306
MvnI CGCG 1 cut(s) 1193
MwoI GCNNNNNNNGC 1 cut(s) 640
NdeII GATC 2 cut(s) 457, 1393
NlaIV GGNNCC 2 cut(s) 136, 1303
NmuCI GTSAC 3 cut(s) 127, 143, 1136
NsiI ATGCAT 1 cut(s) 1202
NspI RCATGY 4 cut(s) 607, 1181, 1200, 1314
PaeI GCATGC 1 cut(s) 607
PaeR7I CTCGAG 1 cut(s) 1122
PagI TCATGA 1 cut(s) 693
PctI GAATGC 1 cut(s) 513
PdmI GAANNNNTTC 1 cut(s) 266
PfeI GAWTC 5 cut(s) 240, 292, 675, 697, 1320
PkrI GCNGC 4 cut(s) 602, 642, 861, 928
PleI GAGTC 2 cut(s) 830, 860
PpsI GAGTC 2 cut(s) 830, 860
PpuMI RGGWCCY 1 cut(s) 1301
PshBI ATTAAT 1 cut(s) 1050
PsiI TTATAA 2 cut(s) 899, 1471
Psp5II RGGWCCY 1 cut(s) 1301
Psp6I CCWGG 3 cut(s) 151, 700, 1304
PspEI GGTNACC 1 cut(s) 165
PspGI CCWGG 3 cut(s) 151, 700, 1304
PspN4I GGNNCC 2 cut(s) 136, 1303
PspPI GGNCC 1 cut(s) 1301
PspPPI RGGWCCY 1 cut(s) 1301
PspXI VCTCGAGB 1 cut(s) 1122
PstI CTGCAG 1 cut(s) 664
PstNI CAGNNNCTG 2 cut(s) 523, 1140
PsuI RGATCY 1 cut(s) 457
PvuII CAGCTG 1 cut(s) 926
RsaI GTAC 4 cut(s) 313, 417, 1144, 1223
RsaNI GTAC 4 cut(s) 312, 416, 1143, 1222
RseI CAYNNNNRTG 2 cut(s) 608, 1378
SalI GTCGAC 1 cut(s) 1388
SaqAI TTAA 4 cut(s) 42, 756, 1050, 1080
SatI GCNGC 4 cut(s) 601, 641, 860, 927
Sau3AI GATC 2 cut(s) 457, 1393
Sau96I GGNCC 1 cut(s) 1301
SchI GAGTC 2 cut(s) 830, 860
ScrFI CCNGG 3 cut(s) 153, 702, 1306
SduI GDGCHC 1 cut(s) 630
SfaNI GCATC 4 cut(s) 212, 580, 732, 1037
SfcI CTRYAG 4 cut(s) 660, 815, 1002, 1146
Sfr274I CTCGAG 1 cut(s) 1122
SinI GGWCC 1 cut(s) 1301
SlaI CTCGAG 1 cut(s) 1122
SmiMI CAYNNNNRTG 2 cut(s) 608, 1378
SmlI CTYRAG 2 cut(s) 644, 1122
SmoI CTYRAG 2 cut(s) 644, 1122
SphI GCATGC 1 cut(s) 607
Sse9I AATT 8 cut(s) 110, 279, 472, 497, 900, 995, 1032, 1238
SspMI CTAG 5 cut(s) 533, 708, 1251, 1409, 1431
StyD4I CCNGG 3 cut(s) 151, 700, 1304
TaaI ACNGT 8 cut(s) 78, 819, 1067, 1090, 1105, 1142, 1361, 1387
TaiI ACGT 1 cut(s) 742
TaqI TCGA 4 cut(s) 501, 1041, 1123, 1389
TasI AATT 8 cut(s) 110, 279, 472, 497, 900, 995, 1032, 1238
TatI WGTACW 2 cut(s) 311, 1142
TfiI GAWTC 5 cut(s) 240, 292, 675, 697, 1320
Tru1I TTAA 4 cut(s) 42, 756, 1050, 1080
Tru9I TTAA 4 cut(s) 42, 756, 1050, 1080
TscAI CASTG 2 cut(s) 664, 1141
TseFI GTSAC 3 cut(s) 127, 143, 1136
TseI GCWGC 4 cut(s) 600, 640, 859, 926
Tsp45I GTSAC 3 cut(s) 127, 143, 1136
TspDTI ATGAA 8 cut(s) 184, 284, 478, 485, 867, 1273, 1275, 1362
TspRI CASTG 2 cut(s) 664, 1141
VpaK11BI GGWCC 1 cut(s) 1301
VspI ATTAAT 1 cut(s) 1050
XagI CCTNNNNNAGG 1 cut(s) 634
XapI RAATTY 4 cut(s) 279, 472, 497, 1238
XbaI TCTAGA 2 cut(s) 1250, 1408
XceI RCATGY 4 cut(s) 607, 1181, 1200, 1314
XcmI CCANNNNNNNNNTGG 1 cut(s) 1312
XhoI CTCGAG 1 cut(s) 1122
XmiI GTMKAC 1 cut(s) 1389
XmnI GAANNNNTTC 1 cut(s) 266
XspI CTAG 5 cut(s) 533, 708, 1251, 1409, 1431
ZraI GACGTC 1 cut(s) 740
Zsp2I ATGCAT 1 cut(s) 1202
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.