Rh2DG431300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0014771
Physical Location & Seq
Reverse (-)
62252705 .. 62254341
1637 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG431300.1

Sequence Viewer

Length: 474 bp
ATGTTTCAGATGATTGACTATATATATGGGCCAGTCTATGCATCAACAATACATCGATCAACAATACTACTGATCGATGATAGTACCACCGCTGGTGTTTTTGGGAAATTTGAGCTCGATCAAGTCTGTTCGTTTTTCATGGCCAAAGTTTGTTGCTCCATTGAGATGGAACCTAGGACCTTGAGTCAAGTACAACTCAACCATGCTAGGGAAATGGCTGAAGATGTGGTTCAGAAAATGGAATCAAACGAAGCCTCAGCTACATTCAGTGAGGGATTGAGACTGATTGGTTCAGTGACACAGGTGGACCATGTAAATATTGCTGAAGCGGGTGAAGAACCACAGAATAAGCTAATTCTGGAGTCGGAGGAGGCTCAACTGCATGAAAGACCATGCCAATGCTTGTGTGCTGCTGCCAATATTGAAACCCCTGATCAAGAAAAGCTTAAGGAACCCCTCTCGGCCCCATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

157

Amino Acids

17.49

Weight (kDa)

4.52

Isoelectric Point (pI)

50.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 90, 329
AcoI YGGCCR 1 cut(s) 141
AcsI RAATTY 1 cut(s) 107
AcuI CTGAAG 2 cut(s) 240, 345
AfaI GTAC 2 cut(s) 85, 192
AfiI CCNNNNNNNGG 1 cut(s) 208
AflII CTTAAG 1 cut(s) 446
AgsI TTSAA 1 cut(s) 425
AhdI GACNNNNNGTC 1 cut(s) 183
AluBI AGCT 4 cut(s) 115, 260, 352, 445
AluI AGCT 4 cut(s) 115, 260, 352, 445
Alw21I GWGCWC 1 cut(s) 117
Alw26I GTCTC 1 cut(s) 274
AoxI GGCC 3 cut(s) 29, 141, 462
ApeKI GCWGC 2 cut(s) 410, 413
ApoI RAATTY 1 cut(s) 107
AspA2I CCTAGG 1 cut(s) 173
AspS9I GGNCC 4 cut(s) 29, 177, 307, 463
AsuHPI GGTGA 1 cut(s) 344
AvaII GGWCC 2 cut(s) 177, 307
AvrII CCTAGG 1 cut(s) 173
BalI TGGCCA 1 cut(s) 143
BanII GRGCYC 1 cut(s) 117
Bbv12I GWGCWC 1 cut(s) 117
BbvCI CCTCAGC 1 cut(s) 256
BbvI GCAGC 2 cut(s) 397, 400
BccI CCATC 1 cut(s) 160
BclI TGATCA 1 cut(s) 433
BcoDI GTCTC 1 cut(s) 274
BfaI CTAG 2 cut(s) 174, 207
BfrI CTTAAG 1 cut(s) 446
BisI GCNGC 2 cut(s) 411, 414
BlnI CCTAGG 1 cut(s) 173
BlsI GCNGC 2 cut(s) 412, 415
Bme18I GGWCC 2 cut(s) 177, 307
BmeRI GACNNNNNGTC 1 cut(s) 183
BmgT120I GGNCC 4 cut(s) 29, 177, 307, 463
BmiI GGNNCC 3 cut(s) 171, 453, 465
BmsI GCATC 1 cut(s) 50
BpmI CTGGAG 1 cut(s) 380
Bpu10I CCTNAGC 1 cut(s) 256
BpuEI CTTGAG 1 cut(s) 202
Bsa29I ATCGAT 2 cut(s) 55, 75
BsaJI CCNNGG 1 cut(s) 173
Bsc4I CCNNNNNNNGG 1 cut(s) 208
Bse1I ACTGG 1 cut(s) 32
BseCI ATCGAT 2 cut(s) 55, 75
BseDI CCNNGG 1 cut(s) 173
BseLI CCNNNNNNNGG 1 cut(s) 208
BseMII CTCAG 1 cut(s) 270
BseNI ACTGG 1 cut(s) 32
BseRI GAGGAG 1 cut(s) 383
BseXI GCAGC 2 cut(s) 397, 400
BshFI GGCC 3 cut(s) 31, 143, 464
BshVI ATCGAT 2 cut(s) 55, 75
BsiHKAI GWGCWC 1 cut(s) 117
BslI CCNNNNNNNGG 1 cut(s) 208
BsmAI GTCTC 1 cut(s) 274
BsnI GGCC 3 cut(s) 31, 143, 464
Bsp1286I GDGCHC 1 cut(s) 117
Bsp143I GATC 4 cut(s) 56, 72, 118, 433
BspACI CCGC 2 cut(s) 90, 329
BspANI GGCC 3 cut(s) 31, 143, 464
BspCNI CTCAG 1 cut(s) 269
BspDI ATCGAT 2 cut(s) 55, 75
BspLI GGNNCC 3 cut(s) 171, 453, 465
BspTI CTTAAG 1 cut(s) 446
BsrI ACTGG 1 cut(s) 32
BssECI CCNNGG 1 cut(s) 173
BssMI GATC 4 cut(s) 56, 72, 118, 433
BssT1I CCWWGG 1 cut(s) 173
BstAFI CTTAAG 1 cut(s) 446
BstDEI CTNAG 1 cut(s) 256
BstKTI GATC 4 cut(s) 59, 75, 121, 436
BstMAI GTCTC 1 cut(s) 274
BstMBI GATC 4 cut(s) 56, 72, 118, 433
BstV1I GCAGC 2 cut(s) 397, 400
BstXI CCANNNNNNTGG 1 cut(s) 166
Bsu15I ATCGAT 2 cut(s) 55, 75
BsuRI GGCC 3 cut(s) 31, 143, 464
BsuTUI ATCGAT 2 cut(s) 55, 75
BtsIMutI CAGTG 2 cut(s) 274, 300
Cfr13I GGNCC 4 cut(s) 29, 177, 307, 463
ClaI ATCGAT 2 cut(s) 55, 75
Csp6I GTAC 2 cut(s) 84, 191
CviAII CATG 5 cut(s) 139, 203, 311, 383, 393
CviQI GTAC 2 cut(s) 84, 191
DdeI CTNAG 1 cut(s) 256
DpnI GATC 4 cut(s) 58, 74, 120, 435
DpnII GATC 4 cut(s) 56, 72, 118, 433
DriI GACNNNNNGTC 1 cut(s) 183
EaeI YGGCCR 1 cut(s) 141
Eam1105I GACNNNNNGTC 1 cut(s) 183
Ecl136II GAGCTC 1 cut(s) 115
Eco130I CCWWGG 1 cut(s) 173
Eco24I GRGCYC 1 cut(s) 117
Eco47I GGWCC 2 cut(s) 177, 307
Eco53kI GAGCTC 1 cut(s) 115
Eco57I CTGAAG 2 cut(s) 240, 345
EcoICRI GAGCTC 1 cut(s) 115
EcoO109I RGGNCCY 1 cut(s) 177
EcoT14I CCWWGG 1 cut(s) 173
EcoT22I ATGCAT 1 cut(s) 43
EcoT38I GRGCYC 1 cut(s) 117
ErhI CCWWGG 1 cut(s) 173
FaeI CATG 5 cut(s) 142, 206, 314, 386, 396
FalI AAGNNNNNCTT 2 cut(s) 429, 461
FatI CATG 5 cut(s) 138, 202, 310, 382, 392
FauI CCCGC 1 cut(s) 322
FbaI TGATCA 1 cut(s) 433
Fnu4HI GCNGC 2 cut(s) 411, 414
FriOI GRGCYC 1 cut(s) 117
Fsp4HI GCNGC 2 cut(s) 411, 414
FspBI CTAG 2 cut(s) 174, 207
GluI GCNGC 2 cut(s) 411, 414
GsuI CTGGAG 1 cut(s) 380
HaeIII GGCC 3 cut(s) 31, 143, 464
Hin1II CATG 5 cut(s) 142, 206, 314, 386, 396
HindIII AAGCTT 1 cut(s) 443
HinfI GANTC 3 cut(s) 184, 242, 362
HphI GGTGA 1 cut(s) 344
Hpy166II GTNNAC 1 cut(s) 307
Hpy188I TCNGA 3 cut(s) 9, 234, 367
Hpy188III TCNNGA 2 cut(s) 359, 437
Hpy8I GTNNAC 1 cut(s) 307
HpyCH4V TGCA 2 cut(s) 41, 382
HpyF3I CTNAG 1 cut(s) 256
Hsp92II CATG 5 cut(s) 142, 206, 314, 386, 396
Ksp22I TGATCA 1 cut(s) 433
Kzo9I GATC 4 cut(s) 56, 72, 118, 433
LmnI GCTCC 1 cut(s) 161
LpnPI CCDG 5 cut(s) 45, 78, 287, 344, 444
Lsp1109I GCAGC 2 cut(s) 397, 400
LweI GCATC 1 cut(s) 50
MaeI CTAG 2 cut(s) 174, 207
MaeIII GTNAC 1 cut(s) 295
MalI GATC 4 cut(s) 58, 74, 120, 435
MboI GATC 4 cut(s) 56, 72, 118, 433
MboII GAAGA 2 cut(s) 233, 347
MhlI GDGCHC 1 cut(s) 117
MlsI TGGCCA 1 cut(s) 143
MluCI AATT 2 cut(s) 107, 354
MluNI TGGCCA 1 cut(s) 143
MlyI GAGTC 2 cut(s) 193, 371
MmeI TCCRAC 1 cut(s) 345
MnlI CCTC 5 cut(s) 265, 265, 361, 364, 467
Mox20I TGGCCA 1 cut(s) 143
Mph1103I ATGCAT 1 cut(s) 43
MscI TGGCCA 1 cut(s) 143
MseI TTAA 1 cut(s) 447
MslI CAYNNNNRTG 2 cut(s) 164, 397
Msp20I TGGCCA 1 cut(s) 143
MspA1I CMGCKG 1 cut(s) 92
MspCI CTTAAG 1 cut(s) 446
NdeII GATC 4 cut(s) 56, 72, 118, 433
NlaIII CATG 5 cut(s) 142, 206, 314, 386, 396
NlaIV GGNNCC 3 cut(s) 171, 453, 465
NmeAIII GCCGAG 1 cut(s) 440
NmuCI GTSAC 1 cut(s) 295
NsiI ATGCAT 1 cut(s) 43
PfeI GAWTC 1 cut(s) 242
PkrI GCNGC 2 cut(s) 412, 415
PleI GAGTC 2 cut(s) 192, 370
PpsI GAGTC 2 cut(s) 192, 370
PpuMI RGGWCCY 1 cut(s) 177
Psp124BI GAGCTC 1 cut(s) 117
Psp5II RGGWCCY 1 cut(s) 177
PspN4I GGNNCC 3 cut(s) 171, 453, 465
PspPI GGNCC 4 cut(s) 29, 177, 307, 463
PspPPI RGGWCCY 1 cut(s) 177
RsaI GTAC 2 cut(s) 85, 192
RsaNI GTAC 2 cut(s) 84, 191
RseI CAYNNNNRTG 2 cut(s) 164, 397
SacI GAGCTC 1 cut(s) 117
SaqAI TTAA 1 cut(s) 447
SatI GCNGC 2 cut(s) 411, 414
Sau3AI GATC 4 cut(s) 56, 72, 118, 433
Sau96I GGNCC 4 cut(s) 29, 177, 307, 463
SchI GAGTC 2 cut(s) 193, 371
SduI GDGCHC 1 cut(s) 117
SetI ASST 7 cut(s) 117, 175, 182, 262, 306, 354, 447
SfaNI GCATC 1 cut(s) 50
SinI GGWCC 2 cut(s) 177, 307
SmiMI CAYNNNNRTG 2 cut(s) 164, 397
SmlI CTYRAG 2 cut(s) 181, 446
SmoI CTYRAG 2 cut(s) 181, 446
Sse9I AATT 2 cut(s) 107, 354
SsiI CCGC 2 cut(s) 90, 329
SspI AATATT 2 cut(s) 319, 421
SspMI CTAG 2 cut(s) 174, 207
SstI GAGCTC 1 cut(s) 117
StyI CCWWGG 1 cut(s) 173
TaqI TCGA 3 cut(s) 55, 75, 117
TasI AATT 2 cut(s) 107, 354
TatI WGTACW 1 cut(s) 190
TfiI GAWTC 1 cut(s) 242
Tru1I TTAA 1 cut(s) 447
Tru9I TTAA 1 cut(s) 447
TscAI CASTG 2 cut(s) 274, 300
TseFI GTSAC 1 cut(s) 295
TseI GCWGC 2 cut(s) 410, 413
Tsp45I GTSAC 1 cut(s) 295
TspDTI ATGAA 2 cut(s) 127, 399
TspRI CASTG 2 cut(s) 274, 300
Vha464I CTTAAG 1 cut(s) 446
VpaK11BI GGWCC 2 cut(s) 177, 307
XapI RAATTY 1 cut(s) 107
XmaJI CCTAGG 1 cut(s) 173
XspI CTAG 2 cut(s) 174, 207
Zsp2I ATGCAT 1 cut(s) 43
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.