Rw2G033720

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0014771
Physical Location & Seq
Reverse (-)
55104854 .. 55106660
1807 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G033720.1

Sequence Viewer

Length: 459 bp
ATGTTTCAGATGATTGACTATATATATGGGCCAGTCTATGCATCAACAATACTACTGATCGATGATAGTACCACCGCTGGTGTCTCTGAGAAATTTGAGCTCGATCAAGTCTGTTCGTTTTTCATGGCCAAAGTTTGTTGCTCCATTGAGATGGAACCTAGGACCTTGAGTCAAGTACAACTCAACCATGCTAGGGAAATGGCTGAAGATGTGGTTCAGAAAATGGAATCAAACGAAGCCTCAGCTACATTCAGTGAGGGATTGAGACCGATTGGTTCAGAGACACAGGTGGACAATGTAAATATTGCTGAAGCGGGTGAAGAACCACAGAATAAGCTAAATCTGGAGTCGGAGGAGGCTCAACTGTATGAAAGATCATGCCAATGCTTGTGTGCTGCTGCCAATATTGAAACCCCTGATCAAGAAAAGCTTAAGGAACCCCTCTCGGCCCCATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

152

Amino Acids

16.91

Weight (kDa)

4.19

Isoelectric Point (pI)

55.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 75, 314
AcoI YGGCCR 1 cut(s) 126
AcsI RAATTY 1 cut(s) 92
AcuI CTGAAG 2 cut(s) 225, 330
AfaI GTAC 2 cut(s) 70, 177
AfiI CCNNNNNNNGG 1 cut(s) 193
AflII CTTAAG 1 cut(s) 431
AgsI TTSAA 1 cut(s) 410
AhdI GACNNNNNGTC 1 cut(s) 168
AluBI AGCT 4 cut(s) 100, 245, 337, 430
AluI AGCT 4 cut(s) 100, 245, 337, 430
Alw21I GWGCWC 1 cut(s) 102
Alw26I GTCTC 3 cut(s) 88, 259, 275
AoxI GGCC 3 cut(s) 29, 126, 447
ApeKI GCWGC 2 cut(s) 395, 398
ApoI RAATTY 1 cut(s) 92
AspA2I CCTAGG 1 cut(s) 158
AspS9I GGNCC 3 cut(s) 29, 162, 448
AsuHPI GGTGA 1 cut(s) 329
AvaII GGWCC 1 cut(s) 162
AvrII CCTAGG 1 cut(s) 158
BalI TGGCCA 1 cut(s) 128
BanII GRGCYC 1 cut(s) 102
Bbv12I GWGCWC 1 cut(s) 102
BbvCI CCTCAGC 1 cut(s) 241
BbvI GCAGC 2 cut(s) 382, 385
BccI CCATC 1 cut(s) 145
BclI TGATCA 1 cut(s) 418
BcoDI GTCTC 3 cut(s) 88, 259, 275
BfaI CTAG 2 cut(s) 159, 192
BfrI CTTAAG 1 cut(s) 431
BisI GCNGC 2 cut(s) 396, 399
BlnI CCTAGG 1 cut(s) 158
BlsI GCNGC 2 cut(s) 397, 400
Bme18I GGWCC 1 cut(s) 162
BmeRI GACNNNNNGTC 1 cut(s) 168
BmgT120I GGNCC 3 cut(s) 29, 162, 448
BmiI GGNNCC 3 cut(s) 156, 438, 450
BmsI GCATC 1 cut(s) 50
BpmI CTGGAG 1 cut(s) 365
Bpu10I CCTNAGC 1 cut(s) 241
BpuEI CTTGAG 1 cut(s) 187
Bsa29I ATCGAT 1 cut(s) 60
BsaI GGTCTC 1 cut(s) 259
BsaJI CCNNGG 1 cut(s) 158
Bsc4I CCNNNNNNNGG 1 cut(s) 193
Bse1I ACTGG 1 cut(s) 32
BseCI ATCGAT 1 cut(s) 60
BseDI CCNNGG 1 cut(s) 158
BseLI CCNNNNNNNGG 1 cut(s) 193
BseMII CTCAG 2 cut(s) 78, 255
BseNI ACTGG 1 cut(s) 32
BseRI GAGGAG 1 cut(s) 368
BseXI GCAGC 2 cut(s) 382, 385
BshFI GGCC 3 cut(s) 31, 128, 449
BshVI ATCGAT 1 cut(s) 60
BsiHKAI GWGCWC 1 cut(s) 102
BslI CCNNNNNNNGG 1 cut(s) 193
BsmAI GTCTC 3 cut(s) 88, 259, 275
BsnI GGCC 3 cut(s) 31, 128, 449
Bso31I GGTCTC 1 cut(s) 259
Bsp1286I GDGCHC 1 cut(s) 102
Bsp143I GATC 4 cut(s) 57, 103, 374, 418
BspACI CCGC 2 cut(s) 75, 314
BspANI GGCC 3 cut(s) 31, 128, 449
BspCNI CTCAG 2 cut(s) 79, 254
BspDI ATCGAT 1 cut(s) 60
BspLI GGNNCC 3 cut(s) 156, 438, 450
BspTI CTTAAG 1 cut(s) 431
BspTNI GGTCTC 1 cut(s) 259
BsrI ACTGG 1 cut(s) 32
BssECI CCNNGG 1 cut(s) 158
BssMI GATC 4 cut(s) 57, 103, 374, 418
BssT1I CCWWGG 1 cut(s) 158
Bst4CI ACNGT 1 cut(s) 366
BstAFI CTTAAG 1 cut(s) 431
BstDEI CTNAG 2 cut(s) 87, 241
BstKTI GATC 4 cut(s) 60, 106, 377, 421
BstMAI GTCTC 3 cut(s) 88, 259, 275
BstMBI GATC 4 cut(s) 57, 103, 374, 418
BstV1I GCAGC 2 cut(s) 382, 385
BstXI CCANNNNNNTGG 1 cut(s) 151
Bsu15I ATCGAT 1 cut(s) 60
BsuRI GGCC 3 cut(s) 31, 128, 449
BsuTUI ATCGAT 1 cut(s) 60
BtsIMutI CAGTG 1 cut(s) 259
Cfr13I GGNCC 3 cut(s) 29, 162, 448
ClaI ATCGAT 1 cut(s) 60
Csp6I GTAC 2 cut(s) 69, 176
CviAII CATG 3 cut(s) 124, 188, 378
CviQI GTAC 2 cut(s) 69, 176
DdeI CTNAG 2 cut(s) 87, 241
DpnI GATC 4 cut(s) 59, 105, 376, 420
DpnII GATC 4 cut(s) 57, 103, 374, 418
DriI GACNNNNNGTC 1 cut(s) 168
EaeI YGGCCR 1 cut(s) 126
Eam1105I GACNNNNNGTC 1 cut(s) 168
Ecl136II GAGCTC 1 cut(s) 100
Eco130I CCWWGG 1 cut(s) 158
Eco24I GRGCYC 1 cut(s) 102
Eco31I GGTCTC 1 cut(s) 259
Eco47I GGWCC 1 cut(s) 162
Eco53kI GAGCTC 1 cut(s) 100
Eco57I CTGAAG 2 cut(s) 225, 330
EcoICRI GAGCTC 1 cut(s) 100
EcoO109I RGGNCCY 1 cut(s) 162
EcoT14I CCWWGG 1 cut(s) 158
EcoT22I ATGCAT 1 cut(s) 43
EcoT38I GRGCYC 1 cut(s) 102
ErhI CCWWGG 1 cut(s) 158
FaeI CATG 3 cut(s) 127, 191, 381
FaiI YATR 9 cut(s) 21, 23, 25, 27, 39, 125, 189, 369, 379
FalI AAGNNNNNCTT 2 cut(s) 414, 446
FatI CATG 3 cut(s) 123, 187, 377
FauI CCCGC 1 cut(s) 307
FbaI TGATCA 1 cut(s) 418
Fnu4HI GCNGC 2 cut(s) 396, 399
FriOI GRGCYC 1 cut(s) 102
Fsp4HI GCNGC 2 cut(s) 396, 399
FspBI CTAG 2 cut(s) 159, 192
GluI GCNGC 2 cut(s) 396, 399
GsuI CTGGAG 1 cut(s) 365
HaeIII GGCC 3 cut(s) 31, 128, 449
Hin1II CATG 3 cut(s) 127, 191, 381
HindIII AAGCTT 1 cut(s) 428
HinfI GANTC 3 cut(s) 169, 227, 347
HphI GGTGA 1 cut(s) 329
Hpy166II GTNNAC 1 cut(s) 292
Hpy188I TCNGA 5 cut(s) 9, 88, 219, 280, 352
Hpy188III TCNNGA 2 cut(s) 344, 422
Hpy8I GTNNAC 1 cut(s) 292
HpyCH4III ACNGT 1 cut(s) 366
HpyCH4V TGCA 1 cut(s) 41
HpyF3I CTNAG 2 cut(s) 87, 241
Hsp92II CATG 3 cut(s) 127, 191, 381
Ksp22I TGATCA 1 cut(s) 418
Kzo9I GATC 4 cut(s) 57, 103, 374, 418
LmnI GCTCC 1 cut(s) 146
LpnPI CCDG 5 cut(s) 45, 63, 272, 329, 429
Lsp1109I GCAGC 2 cut(s) 382, 385
LweI GCATC 1 cut(s) 50
MaeI CTAG 2 cut(s) 159, 192
MalI GATC 4 cut(s) 59, 105, 376, 420
MboI GATC 4 cut(s) 57, 103, 374, 418
MboII GAAGA 2 cut(s) 218, 332
MhlI GDGCHC 1 cut(s) 102
MlsI TGGCCA 1 cut(s) 128
MluCI AATT 1 cut(s) 92
MluNI TGGCCA 1 cut(s) 128
MlyI GAGTC 2 cut(s) 178, 356
MmeI TCCRAC 1 cut(s) 330
MnlI CCTC 5 cut(s) 250, 250, 346, 349, 452
Mox20I TGGCCA 1 cut(s) 128
Mph1103I ATGCAT 1 cut(s) 43
MscI TGGCCA 1 cut(s) 128
MseI TTAA 1 cut(s) 432
MslI CAYNNNNRTG 2 cut(s) 149, 382
Msp20I TGGCCA 1 cut(s) 128
MspA1I CMGCKG 1 cut(s) 77
MspCI CTTAAG 1 cut(s) 431
NdeII GATC 4 cut(s) 57, 103, 374, 418
NlaIII CATG 3 cut(s) 127, 191, 381
NlaIV GGNNCC 3 cut(s) 156, 438, 450
NmeAIII GCCGAG 1 cut(s) 425
NsiI ATGCAT 1 cut(s) 43
PfeI GAWTC 1 cut(s) 227
PkrI GCNGC 2 cut(s) 397, 400
PleI GAGTC 2 cut(s) 177, 355
PpsI GAGTC 2 cut(s) 177, 355
PpuMI RGGWCCY 1 cut(s) 162
Psp124BI GAGCTC 1 cut(s) 102
Psp5II RGGWCCY 1 cut(s) 162
PspN4I GGNNCC 3 cut(s) 156, 438, 450
PspPI GGNCC 3 cut(s) 29, 162, 448
PspPPI RGGWCCY 1 cut(s) 162
RsaI GTAC 2 cut(s) 70, 177
RsaNI GTAC 2 cut(s) 69, 176
RseI CAYNNNNRTG 2 cut(s) 149, 382
SacI GAGCTC 1 cut(s) 102
SaqAI TTAA 1 cut(s) 432
SatI GCNGC 2 cut(s) 396, 399
Sau3AI GATC 4 cut(s) 57, 103, 374, 418
Sau96I GGNCC 3 cut(s) 29, 162, 448
SchI GAGTC 2 cut(s) 178, 356
SduI GDGCHC 1 cut(s) 102
SetI ASST 7 cut(s) 102, 160, 167, 247, 291, 339, 432
SfaNI GCATC 1 cut(s) 50
SinI GGWCC 1 cut(s) 162
SmiMI CAYNNNNRTG 2 cut(s) 149, 382
SmlI CTYRAG 2 cut(s) 166, 431
SmoI CTYRAG 2 cut(s) 166, 431
Sse9I AATT 1 cut(s) 92
SsiI CCGC 2 cut(s) 75, 314
SspI AATATT 2 cut(s) 304, 406
SspMI CTAG 2 cut(s) 159, 192
SstI GAGCTC 1 cut(s) 102
StyI CCWWGG 1 cut(s) 158
TaaI ACNGT 1 cut(s) 366
TaqI TCGA 2 cut(s) 60, 102
TaqII GACCGA 1 cut(s) 283
TasI AATT 1 cut(s) 92
TatI WGTACW 1 cut(s) 175
TfiI GAWTC 1 cut(s) 227
Tru1I TTAA 1 cut(s) 432
Tru9I TTAA 1 cut(s) 432
TscAI CASTG 1 cut(s) 259
TseI GCWGC 2 cut(s) 395, 398
TspDTI ATGAA 2 cut(s) 112, 384
TspRI CASTG 1 cut(s) 259
Vha464I CTTAAG 1 cut(s) 431
VpaK11BI GGWCC 1 cut(s) 162
XapI RAATTY 1 cut(s) 92
XmaJI CCTAGG 1 cut(s) 158
XspI CTAG 2 cut(s) 159, 192
Zsp2I ATGCAT 1 cut(s) 43
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.