Rh2DG610100

ZINC FINGER protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0014778
Physical Location & Seq
Forward (+)
84271409 .. 84273016
1608 bp
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UTR
Exon/CDS
Intron
Rh2DG610100.1

Sequence Viewer

Length: 1608 bp
ATGGAGACGCTGAGAGGTGCAGGGCATGTGTGCAAGTTATGTGGTCAGAGTTGCGTGAGCGGCAAGGTCTTGGGAGGTCACATGAGGTCTCACGCGTTCAAGAATCCGATCAAGTCAAGTAGAAAGCACAAGAAAGCCAACATGGGTTTTCAAGATGACGGCGACGGCAATGTGGGTTATGGCCTGAGATTAAACCCCAAAAGGTCCTGGAAATTTTCAGGCAAAGAAGAGGAAGAAGAGATTGTGTGCAAGGTTTGTGGTAAAGCGTTTGAGAGAAAGAACGCTCTGTTTGGTCACATGAGGCATCACTCGGTGAAGCGGAGGAATGAGGCTCAGTGGTGCAGAGATTGCGGTAAAGGGTTTGAGTCTTTGAAAGCTTTGAGTGTTCATAGGAGAGTTCATGCAAAAAGATTGAAGGGTTTTGATGAAACAGAGAGGGGTTTAACCCAGAAGCTTTGCATTGAGAGTTTGGGGTTGGTGAGGAAGAAAAGATCTGCAAGATTCAGGTACAGGATTGCTTCTCCTAGTTCTTCATTTTCTGGTTTGAATTTGAGTGAATGTAAATTTGGTAATGGGTGTGGAGTAGATATAGAAGAGGCTGCTTTTTGCTTGATGATGATCTCTAGGGGTTTAGGGAATTGGGGTCAGATTAGTTCAGTGGCTGAGTCCTCAAATAACAACTCGTTTTCTGTTGAGGTTGAGTCACCGAGTCAAAGGAAGCGAGTTAGGGACAATGAAGGTGAGGTTTTTGGTTGTGATGGTGATTTTGGGTCTTCACAGATCAAAAGGGCCAAGGTGGAGGTGTCAGCTGATGGTGTTTTGGATTGTGAGAATGGCTCTAGTGTGAAGGAAGTGTCTGAGTTTGGTGAAATTGATTCTGGGTTTGTAGGTGATGAGATGCCTAAATCTGGGTTTGTGTTATGTGGTATTGAAGAATTGGATGTGGAAACTTGTGAGGAGGAAGTAGAAGTGGGTTTATCAGGTTTGGGGTCCATGAAGTTTGGTTTGAGCCAGAAGGCTGTGCTTAAGGCTTGTGATTCTGTGAACAAAGTGGTGCGTACCACTGAATCTGAAACCTTTACTGATTCTGTGAAGAAAAGAGAACAGAAGTGCAGGATATGCAGAAGGGTTTTCGGCACCCATCAGGCACTTGGAGGCCACCAGAGAGTTCATAAGAGAAGTTACAAATGTTCAGTACTGAAGAATGAGGATGGTGACGACAGAACTCAGATTGACATACTTTGTGAGACTGAGGATAATTGCAATCCTGCCAAGCTTAAATGTAGTGATAACTCAGTGGAGCGGGAGATGGAAACTGTGACTGTGGGAGGTTCTGAGAGGAAGGGTCACGTGTGTGGTATCTGCTTGAAGGTTTTTGCATCAGGCCAAGCTTTGGGTGGTCACAAGAGGTCTCATTATGTCAAAGACTCGGAGATAGCCACAGATGAGACCAGAGTAATGAAGCAACAGATTTCTGATATCTGTGATGCATTTGAAGTCAGTCACCAATTGAAACTTCAGGAGGAGAATGATGGTGTTGAATTCAAGCCATGGTGGGCTGGAACTGAACAAAAGCATGAGCTGCTGGTGGGTTTGATCCCCAACTAA

Protein Analysis

535

Amino Acids

59.29

Weight (kDa)

8.19

Isoelectric Point (pI)

48.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C2H2_6 PF13912 450 - 473 2.1e-09 C2H2-type zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014778)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g47240
malus_domestica MD00G1156100.v1.1 MD09G1062700.v1.1
prunus_persica Prupe.3G257500_v2.0.a1
pyrus_communis pycom111g05300 pycom111g05310 pycom17g05670
rosa_chinensis RchiOBHm_Chr2g0166281
rosa_laevigata RLG00000021603
rosa_multiflora Rmu_sc0024214.1_g000001
rosa_roxburghii Rroxscaffold_2G00085020
rosa_rugosa Rorug02G0521800
rosa_samantha Rh2AG587900 Rh2BG599900 Rh2CG570800 Rh2DG610100
rosa_wichuraiana Rw2G049000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1136
AccB7I CCANNNNNTGG 1 cut(s) 1393
AccBSI CCGCTC 2 cut(s) 60, 1303
AccII CGCG 1 cut(s) 95
AciI CCGC 4 cut(s) 60, 319, 351, 1303
AclWI GGATC 1 cut(s) 1591
AcsI RAATTY 4 cut(s) 212, 547, 563, 1541
AcuI CTGAAG 2 cut(s) 1220, 1502
AcvI CACGTG 1 cut(s) 1351
AdeI CACNNNGTG 1 cut(s) 313
AfaI GTAC 3 cut(s) 509, 1060, 1197
AfiI CCNNNNNNNGG 2 cut(s) 908, 1393
AflII CTTAAG 1 cut(s) 1025
AflIII ACRYGT 2 cut(s) 93, 1350
AjnI CCWGG 1 cut(s) 206
AjuI GAANNNNNNNTTGG 4 cut(s) 549, 581, 1500, 1532
AleI CACNNNNGTG 1 cut(s) 1353
AluBI AGCT 6 cut(s) 377, 454, 809, 1276, 1391, 1582
AluI AGCT 6 cut(s) 377, 454, 809, 1276, 1391, 1582
Alw26I GTCTC 4 cut(s) 93, 1241, 1416, 1442
AlwI GGATC 1 cut(s) 1591
AlwNI CAGNNNCTG 1 cut(s) 662
AoxI GGCC 4 cut(s) 181, 789, 1156, 1384
ApeKI GCWGC 2 cut(s) 599, 1582
ApoI RAATTY 4 cut(s) 212, 547, 563, 1541
AspS9I GGNCC 3 cut(s) 204, 789, 990
AsuHPI GGTGA 9 cut(s) 325, 490, 696, 752, 773, 878, 902, 1226, 1496
AvaII GGWCC 2 cut(s) 204, 990
BanI GGYRCC 1 cut(s) 1136
BbrPI CACGTG 1 cut(s) 1351
BbsI GAAGAC 1 cut(s) 765
BbvI GCAGC 2 cut(s) 586, 1569
BccI CCATC 6 cut(s) 752, 806, 1149, 1205, 1303, 1526
BceAI ACGGC 2 cut(s) 175, 181
BciT130I CCWGG 1 cut(s) 208
BcoDI GTCTC 4 cut(s) 93, 1241, 1416, 1442
BfaI CTAG 3 cut(s) 525, 624, 840
BfrI CTTAAG 1 cut(s) 1025
BglII AGATCT 1 cut(s) 491
BisI GCNGC 3 cut(s) 61, 600, 1583
BlsI GCNGC 3 cut(s) 62, 601, 1584
BmcAI AGTACT 1 cut(s) 1197
Bme1390I CCNGG 1 cut(s) 208
Bme18I GGWCC 2 cut(s) 204, 990
BmgT120I GGNCC 3 cut(s) 204, 789, 990
BmiI GGNNCC 2 cut(s) 991, 1138
BmrFI CCNGG 1 cut(s) 208
BmsI GCATC 4 cut(s) 313, 888, 1388, 1477
BpiI GAAGAC 1 cut(s) 765
BplI GAGNNNNNCTC 2 cut(s) 821, 853
BsaAI YACGTR 1 cut(s) 1351
BsaBI GATNNNNATC 1 cut(s) 617
BsaI GGTCTC 3 cut(s) 93, 1416, 1442
BsaJI CCNNGG 2 cut(s) 792, 1550
Bsc4I CCNNNNNNNGG 2 cut(s) 908, 1393
Bse3DI GCAATG 1 cut(s) 175
Bse8I GATNNNNATC 1 cut(s) 617
BseBI CCWGG 1 cut(s) 208
BseDI CCNNGG 2 cut(s) 792, 1550
BseGI GGATG 2 cut(s) 946, 1216
BseJI GATNNNNATC 1 cut(s) 617
BseLI CCNNNNNNNGG 2 cut(s) 908, 1393
BseMI GCAATG 1 cut(s) 175
BseMII CTCAG 8 cut(s) 176, 347, 654, 849, 1241, 1242, 1308, 1326
BseRI GAGGAG 2 cut(s) 971, 1538
BseXI GCAGC 2 cut(s) 586, 1569
BsgI GTGCAG 3 cut(s) 39, 361, 1132
Bsh1236I CGCG 1 cut(s) 95
BshFI GGCC 4 cut(s) 183, 791, 1158, 1386
BshNI GGYRCC 1 cut(s) 1136
BslFI GGGAC 1 cut(s) 743
BslI CCNNNNNNNGG 2 cut(s) 908, 1393
BsmAI GTCTC 4 cut(s) 93, 1241, 1416, 1442
BsmFI GGGAC 1 cut(s) 743
BsnI GGCC 4 cut(s) 183, 791, 1158, 1386
Bso31I GGTCTC 3 cut(s) 93, 1416, 1442
Bsp143I GATC 5 cut(s) 108, 491, 618, 780, 1596
Bsp19I CCATGG 1 cut(s) 1550
BspACI CCGC 4 cut(s) 60, 319, 351, 1303
BspANI GGCC 4 cut(s) 183, 791, 1158, 1386
BspCNI CTCAG 8 cut(s) 177, 346, 655, 850, 1240, 1243, 1307, 1327
BspFNI CGCG 1 cut(s) 95
BspLI GGNNCC 2 cut(s) 991, 1138
BspPI GGATC 1 cut(s) 1591
BspT107I GGYRCC 1 cut(s) 1136
BspTI CTTAAG 1 cut(s) 1025
BspTNI GGTCTC 3 cut(s) 93, 1416, 1442
BsrBI CCGCTC 2 cut(s) 60, 1303
BsrDI GCAATG 1 cut(s) 175
BssECI CCNNGG 2 cut(s) 792, 1550
BssMI GATC 5 cut(s) 108, 491, 618, 780, 1596
BssT1I CCWWGG 2 cut(s) 792, 1550
Bst2UI CCWGG 1 cut(s) 208
Bst4CI ACNGT 2 cut(s) 1318, 1324
Bst6I CTCTTC 3 cut(s) 222, 231, 588
BstAFI CTTAAG 1 cut(s) 1025
BstAPI GCANNNNNTGC 3 cut(s) 348, 1119, 1582
BstBAI YACGTR 1 cut(s) 1351
BstDEI CTNAG 9 cut(s) 11, 185, 333, 663, 858, 1227, 1251, 1294, 1335
BstDSI CCRYGG 1 cut(s) 1550
BstF5I GGATG 2 cut(s) 946, 1216
BstFNI CGCG 1 cut(s) 95
BstKTI GATC 5 cut(s) 111, 494, 621, 783, 1599
BstMAI GTCTC 4 cut(s) 93, 1241, 1416, 1442
BstMBI GATC 5 cut(s) 108, 491, 618, 780, 1596
BstMWI GCNNNNNNNGC 4 cut(s) 60, 348, 1119, 1582
BstNI CCWGG 1 cut(s) 208
BstNSI RCATGY 1 cut(s) 29
BstSCI CCNGG 1 cut(s) 206
BstUI CGCG 1 cut(s) 95
BstV1I GCAGC 2 cut(s) 586, 1569
BstV2I GAAGAC 1 cut(s) 765
BstX2I RGATCY 1 cut(s) 491
BstYI RGATCY 1 cut(s) 491
BsuRI GGCC 4 cut(s) 183, 791, 1158, 1386
BtgI CCRYGG 1 cut(s) 1550
BtsCI GGATG 2 cut(s) 946, 1216
BtsIMutI CAGTG 4 cut(s) 341, 663, 1062, 1302
CaiI CAGNNNCTG 1 cut(s) 662
Cfr13I GGNCC 3 cut(s) 204, 789, 990
CseI GACGC 1 cut(s) 16
Csp6I GTAC 3 cut(s) 508, 1059, 1196
CspCI CAANNNNNGTGG 4 cut(s) 22, 57, 238, 273
CviAII CATG 8 cut(s) 26, 82, 142, 298, 401, 994, 1551, 1577
CviQI GTAC 3 cut(s) 508, 1059, 1196
DdeI CTNAG 9 cut(s) 11, 185, 333, 663, 858, 1227, 1251, 1294, 1335
DpnI GATC 5 cut(s) 110, 493, 620, 782, 1598
DpnII GATC 5 cut(s) 108, 491, 618, 780, 1596
DraIII CACNNNGTG 1 cut(s) 313
Eam1104I CTCTTC 3 cut(s) 222, 231, 588
EarI CTCTTC 3 cut(s) 222, 231, 588
Eco130I CCWWGG 2 cut(s) 792, 1550
Eco31I GGTCTC 3 cut(s) 93, 1416, 1442
Eco32I GATATC 1 cut(s) 1480
Eco47I GGWCC 2 cut(s) 204, 990
Eco57I CTGAAG 2 cut(s) 1220, 1502
Eco72I CACGTG 1 cut(s) 1351
EcoO109I RGGNCCY 1 cut(s) 204
EcoRI GAATTC 1 cut(s) 1541
EcoRII CCWGG 1 cut(s) 206
EcoRV GATATC 1 cut(s) 1480
EcoT14I CCWWGG 2 cut(s) 792, 1550
EcoT22I ATGCAT 1 cut(s) 1492
ErhI CCWWGG 2 cut(s) 792, 1550
FaeI CATG 8 cut(s) 29, 85, 145, 301, 404, 997, 1554, 1580
FaqI GGGAC 1 cut(s) 743
FatI CATG 8 cut(s) 25, 81, 141, 297, 400, 993, 1550, 1576
FauI CCCGC 1 cut(s) 1296
Fnu4HI GCNGC 3 cut(s) 61, 600, 1583
FokI GGATG 2 cut(s) 953, 1223
Fsp4HI GCNGC 3 cut(s) 61, 600, 1583
FspBI CTAG 3 cut(s) 525, 624, 840
GluI GCNGC 3 cut(s) 61, 600, 1583
HaeIII GGCC 4 cut(s) 183, 791, 1158, 1386
HgaI GACGC 1 cut(s) 16
Hin1II CATG 8 cut(s) 29, 85, 145, 301, 404, 997, 1554, 1580
HindIII AAGCTT 4 cut(s) 375, 452, 1274, 1389
HphI GGTGA 9 cut(s) 325, 490, 696, 752, 773, 878, 902, 1226, 1496
Hpy166II GTNNAC 1 cut(s) 1045
Hpy188I TCNGA 9 cut(s) 48, 108, 648, 859, 1072, 1230, 1336, 1432, 1477
Hpy188III TCNNGA 3 cut(s) 100, 152, 1520
Hpy8I GTNNAC 1 cut(s) 1045
Hpy99I CGWCG 1 cut(s) 167
HpyAV CCTTC 7 cut(s) 409, 731, 841, 1009, 1119, 1336, 1363
HpyCH4III ACNGT 2 cut(s) 1318, 1324
HpyCH4IV ACGT 1 cut(s) 1350
HpyF10VI GCNNNNNNNGC 4 cut(s) 60, 348, 1119, 1582
HpyF3I CTNAG 9 cut(s) 11, 185, 333, 663, 858, 1227, 1251, 1294, 1335
HpySE526I ACGT 1 cut(s) 1350
Hsp92II CATG 8 cut(s) 29, 85, 145, 301, 404, 997, 1554, 1580
Kzo9I GATC 5 cut(s) 108, 491, 618, 780, 1596
LmnI GCTCC 1 cut(s) 1300
Lsp1109I GCAGC 2 cut(s) 586, 1569
LweI GCATC 4 cut(s) 313, 888, 1388, 1477
MaeI CTAG 3 cut(s) 525, 624, 840
MaeII ACGT 1 cut(s) 1350
MaeIII GTNAC 9 cut(s) 77, 293, 702, 1181, 1214, 1318, 1346, 1400, 1502
MalI GATC 5 cut(s) 110, 493, 620, 782, 1598
MbiI CCGCTC 2 cut(s) 60, 1303
MboI GATC 5 cut(s) 108, 491, 618, 780, 1596
MfeI CAATTG 1 cut(s) 1508
MflI RGATCY 1 cut(s) 491
MluCI AATT 9 cut(s) 212, 547, 563, 637, 870, 935, 1258, 1508, 1541
MluI ACGCGT 1 cut(s) 93
MlyI GAGTC 5 cut(s) 374, 674, 710, 718, 1421
Mph1103I ATGCAT 1 cut(s) 1492
MseI TTAA 4 cut(s) 191, 443, 1026, 1278
MslI CAYNNNNRTG 1 cut(s) 1353
MspA1I CMGCKG 1 cut(s) 809
MspCI CTTAAG 1 cut(s) 1025
MspR9I CCNGG 1 cut(s) 208
MunI CAATTG 1 cut(s) 1508
MvaI CCWGG 1 cut(s) 208
MvnI CGCG 1 cut(s) 95
MwoI GCNNNNNNNGC 4 cut(s) 60, 348, 1119, 1582
NcoI CCATGG 1 cut(s) 1550
NdeII GATC 5 cut(s) 108, 491, 618, 780, 1596
NlaIII CATG 8 cut(s) 29, 85, 145, 301, 404, 997, 1554, 1580
NlaIV GGNNCC 2 cut(s) 991, 1138
NmuCI GTSAC 8 cut(s) 77, 293, 702, 1214, 1318, 1346, 1400, 1502
NsiI ATGCAT 1 cut(s) 1492
NspI RCATGY 1 cut(s) 29
OliI CACNNNNGTG 1 cut(s) 1353
PfeI GAWTC 6 cut(s) 103, 501, 875, 1037, 1067, 1085
PflMI CCANNNNNTGG 1 cut(s) 1393
PfoI TCCNGGA 1 cut(s) 206
PkrI GCNGC 3 cut(s) 62, 601, 1584
PleI GAGTC 5 cut(s) 373, 673, 709, 717, 1421
PmaCI CACGTG 1 cut(s) 1351
PmlI CACGTG 1 cut(s) 1351
PpsI GAGTC 5 cut(s) 373, 673, 709, 717, 1421
Ppu21I YACGTR 1 cut(s) 1351
PpuMI RGGWCCY 1 cut(s) 204
Psp5II RGGWCCY 1 cut(s) 204
Psp6I CCWGG 1 cut(s) 206
PspCI CACGTG 1 cut(s) 1351
PspGI CCWGG 1 cut(s) 206
PspN4I GGNNCC 2 cut(s) 991, 1138
PspPI GGNCC 3 cut(s) 204, 789, 990
PspPPI RGGWCCY 1 cut(s) 204
PstNI CAGNNNCTG 1 cut(s) 662
PsuI RGATCY 1 cut(s) 491
PvuII CAGCTG 1 cut(s) 809
RsaI GTAC 3 cut(s) 509, 1060, 1197
RsaNI GTAC 3 cut(s) 508, 1059, 1196
RseI CAYNNNNRTG 1 cut(s) 1353
SaqAI TTAA 4 cut(s) 191, 443, 1026, 1278
SatI GCNGC 3 cut(s) 61, 600, 1583
Sau3AI GATC 5 cut(s) 108, 491, 618, 780, 1596
Sau96I GGNCC 3 cut(s) 204, 789, 990
ScaI AGTACT 1 cut(s) 1197
SchI GAGTC 5 cut(s) 374, 674, 710, 718, 1421
ScrFI CCNGG 1 cut(s) 208
SfaNI GCATC 4 cut(s) 313, 888, 1388, 1477
SinI GGWCC 2 cut(s) 204, 990
SmiMI CAYNNNNRTG 1 cut(s) 1353
SmlI CTYRAG 1 cut(s) 1025
SmoI CTYRAG 1 cut(s) 1025
Sse9I AATT 9 cut(s) 212, 547, 563, 637, 870, 935, 1258, 1508, 1541
SsiI CCGC 4 cut(s) 60, 319, 351, 1303
SspMI CTAG 3 cut(s) 525, 624, 840
StyD4I CCNGG 1 cut(s) 206
StyI CCWWGG 2 cut(s) 792, 1550
TaaI ACNGT 2 cut(s) 1318, 1324
TaiI ACGT 1 cut(s) 1353
TasI AATT 9 cut(s) 212, 547, 563, 637, 870, 935, 1258, 1508, 1541
TatI WGTACW 1 cut(s) 1195
TauI GCSGC 1 cut(s) 63
TfiI GAWTC 6 cut(s) 103, 501, 875, 1037, 1067, 1085
Tru1I TTAA 4 cut(s) 191, 443, 1026, 1278
Tru9I TTAA 4 cut(s) 191, 443, 1026, 1278
TscAI CASTG 4 cut(s) 341, 663, 1069, 1302
TseFI GTSAC 8 cut(s) 77, 293, 702, 1214, 1318, 1346, 1400, 1502
TseI GCWGC 2 cut(s) 599, 1582
Tsp45I GTSAC 8 cut(s) 77, 293, 702, 1214, 1318, 1346, 1400, 1502
TspDTI ATGAA 8 cut(s) 377, 389, 441, 522, 750, 1010, 1160, 1475
TspRI CASTG 4 cut(s) 341, 663, 1069, 1302
Van91I CCANNNNNTGG 1 cut(s) 1393
Vha464I CTTAAG 1 cut(s) 1025
VpaK11BI GGWCC 2 cut(s) 204, 990
XapI RAATTY 4 cut(s) 212, 547, 563, 1541
XceI RCATGY 1 cut(s) 29
XcmI CCANNNNNNNNNTGG 2 cut(s) 1148, 1394
XspI CTAG 3 cut(s) 525, 624, 840
ZrmI AGTACT 1 cut(s) 1197
Zsp2I ATGCAT 1 cut(s) 1492
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.