Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3D

Orthogroup ID

OG0011921

Physical Location & Seq

Genomic Coordinates Forward (+)

20718263 .. 20722394

Gene Structure 4132 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3DG221100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1923 bp

ATGATAGGTTGGGTTGTTGGCGGTGGAGCCTATGTGTTGATATGGGGTTCTTGCAAGAGGATTGAGAGTGGTGGTGGCGGCGGGTTTGTTTGGGATGGTGGTGGATATCTCATAGTGGTTTCATTATGGTGTTCTCTGCAAATTAGATTTAGTGCAGGAGAAGTTAATGGTGGGAATATTAATCCAGTGGCAGTGGCTCTTCGCGGTCCAATGACTTCTTCTCTACAGATGATTGAGAACTATGGTGAAGAAGAAGAAGGGGGGAAACGAAAATGCAAAGAGTCCTATATGCCTAGTATGCGCGTAGCTTCAGCCTCAATCGCCACATTACTCAAAGCCTGCAAAACCCAGTTCCGTCTTGAACAAATCCACGCCCAGATCATCCACAAAGGTCTAGAGCAAGACCACTTCCTCATCACCCAATTCATCTGCCTCTCTAACGCTCTCTCCTCCCTCTCTTACTCCACCGCCGTCTTCCGCCGCGTCAACAACCCCAGCAGCCATCTCTGGAACTCACTACTCAAAGGCCGCTGCGAAAGATCCGGTTTTTCCGCCACCGTCTCTCTCTTCATTCGCATGAAGAGGGAGGAGGGCGCGGCGGATGAGTACACGTACCCGTCGGTTGTGAAGGCCTGTGCCGGCGAGGGAAGGGTCCGGGAGGGAAGGGCGGTTCATGGGTCGGCGGTGAGGTGTGGGGTTGATGGGAATGTGTTTGTGGGGACTAGCTTGGTCGACTTGTATGGCAAATGTAGGGAGGTTGTGTGTGCACGGAAGGTGTTTGATGGAATGTGTGAGAGAAATGTGGTGTCTTGGACTGCTATGGTTGTTGGGTATGCCGGTGTTGGGGATATGGTGGGGGCGAGTAAGGTGTTCGGTGAAATGCCGGAGAGGAATGCTGTGTCGTGGAATGTGGTTATTAGTGGGTTTGTTAGGTTGGGTGATTTGGGGAATGCGAGGAGGGTGTTTGAGGAGATGCCGGGGAAAGATGTGGTTTCTTTTACTACTATGATTGATGGATATGCAAAGTCCGGGGATATGGCTTCGGCCAGGTTTTTGTTTGAGCAGGCTCCCGTTAAGGATATTGTTATGTGGTCGGCGTTGATATCGGGGTATGCTCAAAATGGTTTTCCCAATCAGGCTGTGAAAATATTTCTTGAGATGAGTAGTAGGAATGTGGAGCCTGATGAGTTTATAATGGTGAGCTTGATGTCAGCTTGCTCCCAAGTGGGTTGCTTGGAATTGGCTGAATGGGTTGATTCGTATCTGAGCAATAGCTCAATCGATATTCGACAGCCTCATGTTCTTGCGGCTCTGATTGACATGAATGCAAAGTGTGGGAATATGGAAAGAGCAACGAGTTTGTTTGAAAACATGCCAAAACGGGACCTAATTTCGTATTGTTCAATGATACAAGGGTTGTCTATTCACGGCCGAGGGAATCAAGCTGTTGCCCTTTTCAATAGGATGTTAAGTGAAGGTTTGGCTCCAGATGAGGTAGCTTTTACAGTGATCCTGACAGCTTGTAGCCGTTCCGAGCTTGTTGAGGAGGGTCGGCATTTCTTTGAAATAATGAGACATAAATACAGTATGGTTCCCACTCCTGATCACTATGCATGCATGGTTGATCTTCTTAGCCGGTCAGGATGGTTAGAAGAAGCTTATGAGCTTCTACAATCAATGCCTGTGGAACCTCATGCTGGTGCTTGGGGTGCACTACTTGGTGCATGTAAGCTACATTGCAATGTTGAGTTTGGGGAGTTGGTTGCTAATCGACTCTTTGTGCTTGAACCAAAAAGTGCTGGTAGTTATGTGCTCCTCTCCGACATATATGCTGCATTCCACCGCTGGTTGGATGTTTCTGTTGTGAGGAACAAAATGCAGGAGAGAGGCCTTAAAAAGATACCTGGTATCAGTTCAATTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2

Protein Analysis

640

Amino Acids

70.24

Weight (kDa)

6.52

Isoelectric Point (pI)

46.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR	PF01535	300 - 329	5.5e-06	PPR repeat
PPR_2	PF13041	359 - 406	7e-09	PPR repeat family
PPR	PF01535	362 - 392	4.2e-07	PPR repeat
PPR_2	PF13041	462 - 509	1.2e-09	PPR repeat family
E_motif	PF20431	579 - 640	7.1e-19	E motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011921)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G37570 AT5G37570
fragaria_vesca	FvH4_6g19660
malus_domestica	MD02G1238600.v1.1 MD07G1078100.v1.1
prunus_persica	Prupe.1G026700_v2.0.a1 Prupe.8G050400_v2.0.a1
pyrus_communis	pycom02g20390 pycom07g06180
rosa_chinensis	RchiOBHm_Chr3g0475001
rosa_laevigata	RLG00000023872
rosa_multiflora	Rmu_sc0003093.1_g000009
rosa_roxburghii	Rroxscaffold_6G00406640
rosa_rugosa	Rorug03G0146000
rosa_samantha	Rh3AG195600 Rh3BG225800 Rh3CG220600 Rh3DG221100
rosa_wichuraiana	Rw3G017810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1193
AccI	GTMKAC	1 cut(s)	732
AccII	CGCG	4 cut(s)	204, 303, 483, 596
AclWI	GGATC	2 cut(s)	534, 1504
AcoI	YGGCCR	2 cut(s)	1044, 1429
AcuI	CTGAAG	1 cut(s)	294
AfaI	GTAC	2 cut(s)	608, 614
AfiI	CCNNNNNNNGG	3 cut(s)	843, 1697, 1849
AflIII	ACRYGT	1 cut(s)	609
AgsI	TTSAA	7 cut(s)	362, 1367, 1404, 1459, 1565, 1787, 1917
AjnI	CCWGG	2 cut(s)	1046, 1903
AjuI	GAANNNNNNNTTGG	2 cut(s)	202, 234
AloI	GAACNNNNNNTCC	2 cut(s)	654, 686
Alw21I	GWGCWC	3 cut(s)	769, 1714, 1815
Alw26I	GTCTC	2 cut(s)	565, 1567
Alw44I	GTGCAC	2 cut(s)	765, 1710
AlwI	GGATC	2 cut(s)	534, 1504
AoxI	GGCC	5 cut(s)	526, 630, 1044, 1429, 1888
ApaLI	GTGCAC	2 cut(s)	765, 1710
ApeKI	GCWGC	3 cut(s)	498, 531, 1832
AseI	ATTAAT	1 cut(s)	180
AspLEI	GCGC	2 cut(s)	303, 596
AspS9I	GGNCC	3 cut(s)	206, 652, 1384
AsuC2I	CCSGG	3 cut(s)	656, 978, 1030
AsuHPI	GGTGA	6 cut(s)	257, 409, 697, 887, 950, 1210
AvaII	GGWCC	3 cut(s)	206, 652, 1384
BaeGI	GKGCMC	2 cut(s)	769, 1714
BaeI	ACNNNNGTAYC	2 cut(s)	1400, 1433
BbsI	GAAGAC	1 cut(s)	466
Bbv12I	GWGCWC	3 cut(s)	769, 1714, 1815
BbvI	GCAGC	3 cut(s)	510, 518, 1819
BccI	CCATC	6 cut(s)	89, 510, 695, 776, 1007, 1638
BceAI	ACGGC	3 cut(s)	455, 1444, 1512
BcgI	CGANNNNNNTGC	2 cut(s)	1811, 1845
BciT130I	CCWGG	2 cut(s)	1048, 1905
BclI	TGATCA	1 cut(s)	1603
BcnI	CCSGG	3 cut(s)	656, 978, 1030
BcoDI	GTCTC	2 cut(s)	565, 1567
BfaI	CTAG	3 cut(s)	294, 395, 723
BfmI	CTRYAG	1 cut(s)	224
BisI	GCNGC	8 cut(s)	79, 481, 499, 529, 532, 597, 1308, 1833
BlsI	GCNGC	8 cut(s)	80, 482, 500, 530, 533, 598, 1309, 1834
Bme1390I	CCNGG	5 cut(s)	656, 978, 1030, 1048, 1905
Bme18I	GGWCC	3 cut(s)	206, 652, 1384
BmgT120I	GGNCC	3 cut(s)	206, 652, 1384
BmiI	GGNNCC	8 cut(s)	28, 653, 1068, 1179, 1385, 1485, 1593, 1689
BmrFI	CCNGG	5 cut(s)	656, 978, 1030, 1048, 1905
BmrI	ACTGGG	1 cut(s)	343
BmsI	GCATC	1 cut(s)	963
BmuI	ACTGGG	1 cut(s)	343
BpiI	GAAGAC	1 cut(s)	466
BpmI	CTGGAG	1 cut(s)	1470
BpuEI	CTTGAG	1 cut(s)	1175
BpuMI	CCSGG	3 cut(s)	656, 978, 1030
Bsa29I	ATCGAT	1 cut(s)	1281
BsaAI	YACGTR	1 cut(s)	612
BsaBI	GATNNNNATC	1 cut(s)	1260
BsaJI	CCNNGG	3 cut(s)	977, 1029, 1432
BsaWI	WCCGGW	1 cut(s)	542
BsaXI	ACNNNNNCTCC	6 cut(s)	18, 48, 431, 461, 746, 776
Bsc4I	CCNNNNNNNGG	3 cut(s)	843, 1697, 1849
Bse118I	RCCGGY	3 cut(s)	638, 836, 1635
Bse1I	ACTGG	2 cut(s)	185, 349
Bse3DI	GCAATG	2 cut(s)	1735, 1747
Bse8I	GATNNNNATC	1 cut(s)	1260
BseBI	CCWGG	2 cut(s)	1048, 1905
BseCI	ATCGAT	1 cut(s)	1281
BseDI	CCNNGG	3 cut(s)	977, 1029, 1432
BseGI	GGATG	6 cut(s)	100, 381, 607, 1470, 1649, 1858
BseJI	GATNNNNATC	1 cut(s)	1260
BseLI	CCNNNNNNNGG	3 cut(s)	843, 1697, 1849
BseMI	GCAATG	2 cut(s)	1735, 1747
BseMII	CTCAG	1 cut(s)	1256
BseNI	ACTGG	2 cut(s)	185, 349
BseRI	GAGGAG	6 cut(s)	439, 602, 970, 983, 1559, 1805
BseSI	GKGCMC	2 cut(s)	769, 1714
BseX3I	CGGCCG	1 cut(s)	1429
BseXI	GCAGC	3 cut(s)	510, 518, 1819
BseYI	CCCAGC	1 cut(s)	494
BsgI	GTGCAG	1 cut(s)	174
Bsh1236I	CGCG	4 cut(s)	204, 303, 483, 596
Bsh1285I	CGRYCG	1 cut(s)	1432
BshFI	GGCC	5 cut(s)	528, 632, 1046, 1431, 1890
BshVI	ATCGAT	1 cut(s)	1281
BsiEI	CGRYCG	1 cut(s)	1432
BsiHKAI	GWGCWC	3 cut(s)	769, 1714, 1815
BsiSI	CCGG	8 cut(s)	543, 639, 655, 837, 884, 977, 1029, 1636
BslFI	GGGAC	2 cut(s)	733, 1397
BslI	CCNNNNNNNGG	3 cut(s)	843, 1697, 1849
BsmAI	GTCTC	2 cut(s)	565, 1567
BsmBI	CGTCTC	1 cut(s)	565
BsmFI	GGGAC	2 cut(s)	733, 1397
BsmI	GAATGC	4 cut(s)	898, 955, 1330, 1835
BsnI	GGCC	5 cut(s)	528, 632, 1046, 1431, 1890
Bsp1286I	GDGCHC	3 cut(s)	769, 1714, 1815
Bsp143I	GATC	5 cut(s)	378, 539, 1509, 1603, 1624
BspANI	GGCC	5 cut(s)	528, 632, 1046, 1431, 1890
BspCNI	CTCAG	1 cut(s)	1257
BspDI	ATCGAT	1 cut(s)	1281
BspFNI	CGCG	4 cut(s)	204, 303, 483, 596
BspLI	GGNNCC	8 cut(s)	28, 653, 1068, 1179, 1385, 1485, 1593, 1689
BspPI	GGATC	2 cut(s)	534, 1504
BspQI	GCTCTTC	1 cut(s)	204
BsrDI	GCAATG	2 cut(s)	1735, 1747
BsrFI	RCCGGY	3 cut(s)	638, 836, 1635
BsrI	ACTGG	2 cut(s)	185, 349
BssAI	RCCGGY	3 cut(s)	638, 836, 1635
BssECI	CCNNGG	3 cut(s)	977, 1029, 1432
BssMI	GATC	5 cut(s)	378, 539, 1509, 1603, 1624
Bst2UI	CCWGG	2 cut(s)	1048, 1905
Bst4CI	ACNGT	3 cut(s)	559, 1507, 1586
Bst6I	CTCTTC	3 cut(s)	204, 572, 575
BstBAI	YACGTR	1 cut(s)	612
BstC8I	GCNNGC	5 cut(s)	340, 640, 1065, 1216, 1615
BstDEI	CTNAG	2 cut(s)	1265, 1631
BstF5I	GGATG	6 cut(s)	100, 381, 607, 1470, 1649, 1858
BstFNI	CGCG	4 cut(s)	204, 303, 483, 596
BstHHI	GCGC	2 cut(s)	303, 596
BstKTI	GATC	5 cut(s)	381, 542, 1512, 1606, 1627
BstMAI	GTCTC	2 cut(s)	565, 1567
BstMBI	GATC	5 cut(s)	378, 539, 1509, 1603, 1624
BstMCI	CGRYCG	1 cut(s)	1432
BstMWI	GCNNNNNNNGC	3 cut(s)	298, 320, 1709
BstNI	CCWGG	2 cut(s)	1048, 1905
BstNSI	RCATGY	3 cut(s)	1375, 1617, 1728
BstSCI	CCNGG	5 cut(s)	654, 976, 1028, 1046, 1903
BstSFI	CTRYAG	1 cut(s)	224
BstSLI	GKGCMC	2 cut(s)	769, 1714
BstUI	CGCG	4 cut(s)	204, 303, 483, 596
BstV1I	GCAGC	3 cut(s)	510, 518, 1819
BstV2I	GAAGAC	1 cut(s)	466
BstX2I	RGATCY	1 cut(s)	539
BstYI	RGATCY	1 cut(s)	539
BstZI	CGGCCG	1 cut(s)	1429
Bsu15I	ATCGAT	1 cut(s)	1281
BsuRI	GGCC	5 cut(s)	528, 632, 1046, 1431, 1890
BsuTUI	ATCGAT	1 cut(s)	1281
BtsCI	GGATG	6 cut(s)	100, 381, 607, 1470, 1649, 1858
BtsI	GCAGTG	1 cut(s)	198
BtsIMutI	CAGTG	3 cut(s)	192, 198, 1512
Cac8I	GCNNGC	5 cut(s)	340, 640, 1065, 1216, 1615
CfoI	GCGC	2 cut(s)	303, 596
Cfr10I	RCCGGY	3 cut(s)	638, 836, 1635
Cfr13I	GGNCC	3 cut(s)	206, 652, 1384
ClaI	ATCGAT	1 cut(s)	1281
CseI	GACGC	1 cut(s)	472
CsiI	ACCWGGT	1 cut(s)	1903
Csp6I	GTAC	2 cut(s)	607, 613
CspCI	CAANNNNNGTGG	2 cut(s)	1665, 1700
CviAII	CATG	9 cut(s)	577, 674, 1298, 1321, 1372, 1614, 1618, 1694, 1725
CviQI	GTAC	2 cut(s)	607, 613
DdeI	CTNAG	2 cut(s)	1265, 1631
DpnI	GATC	5 cut(s)	380, 541, 1511, 1605, 1626
DpnII	GATC	5 cut(s)	378, 539, 1509, 1603, 1624
EaeI	YGGCCR	2 cut(s)	1044, 1429
EagI	CGGCCG	1 cut(s)	1429
Eam1104I	CTCTTC	3 cut(s)	204, 572, 575
EarI	CTCTTC	3 cut(s)	204, 572, 575
EciI	GGCGGA	3 cut(s)	467, 541, 614
EclXI	CGGCCG	1 cut(s)	1429
Eco147I	AGGCCT	2 cut(s)	632, 1890
Eco32I	GATATC	2 cut(s)	107, 1104
Eco47I	GGWCC	3 cut(s)	206, 652, 1384
Eco52I	CGGCCG	1 cut(s)	1429
Eco57I	CTGAAG	1 cut(s)	294
EcoO109I	RGGNCCY	1 cut(s)	1384
EcoRII	CCWGG	2 cut(s)	1046, 1903
EcoRV	GATATC	2 cut(s)	107, 1104
EcoT22I	ATGCAT	2 cut(s)	1615, 1619
Esp3I	CGTCTC	1 cut(s)	565
FaeI	CATG	9 cut(s)	580, 677, 1301, 1324, 1375, 1617, 1621, 1697, 1728
FaqI	GGGAC	2 cut(s)	733, 1397
FatI	CATG	9 cut(s)	576, 673, 1297, 1320, 1371, 1613, 1617, 1693, 1724
FauI	CCCGC	1 cut(s)	74
FbaI	TGATCA	1 cut(s)	1603
FblI	GTMKAC	1 cut(s)	732
Fnu4HI	GCNGC	8 cut(s)	79, 481, 499, 529, 532, 597, 1308, 1833
FokI	GGATG	6 cut(s)	107, 368, 614, 1477, 1656, 1865
Fsp4HI	GCNGC	8 cut(s)	79, 481, 499, 529, 532, 597, 1308, 1833
FspBI	CTAG	3 cut(s)	294, 395, 723
GlaI	GCGC	2 cut(s)	302, 595
GluI	GCNGC	8 cut(s)	79, 481, 499, 529, 532, 597, 1308, 1833
GsaI	CCCAGC	1 cut(s)	498
GsuI	CTGGAG	1 cut(s)	1470
HaeIII	GGCC	5 cut(s)	528, 632, 1046, 1431, 1890
HapII	CCGG	8 cut(s)	543, 639, 655, 837, 884, 977, 1029, 1636
HgaI	GACGC	1 cut(s)	472
HhaI	GCGC	2 cut(s)	303, 596
Hin1II	CATG	9 cut(s)	580, 677, 1301, 1324, 1375, 1617, 1621, 1697, 1728
Hin6I	GCGC	2 cut(s)	301, 594
HinP1I	GCGC	2 cut(s)	301, 594
HincII	GTYRAC	2 cut(s)	487, 733
HindII	GTYRAC	2 cut(s)	487, 733
HindIII	AAGCTT	1 cut(s)	1656
HinfI	GANTC	4 cut(s)	281, 1256, 1438, 1773
HpaII	CCGG	8 cut(s)	543, 639, 655, 837, 884, 977, 1029, 1636
HphI	GGTGA	6 cut(s)	257, 409, 697, 887, 950, 1210
Hpy166II	GTNNAC	5 cut(s)	487, 609, 733, 767, 1712
Hpy188I	TCNGA	4 cut(s)	1266, 1314, 1534, 1822
Hpy188III	TCNNGA	8 cut(s)	359, 395, 508, 1154, 1487, 1513, 1601, 1641
Hpy8I	GTNNAC	5 cut(s)	487, 609, 733, 767, 1712
Hpy99I	CGWCG	1 cut(s)	622
HpyAV	CCTTC	6 cut(s)	251, 622, 642, 657, 766, 1469
HpyCH4III	ACNGT	3 cut(s)	559, 1507, 1586
HpyCH4IV	ACGT	1 cut(s)	611
HpyF10VI	GCNNNNNNNGC	3 cut(s)	298, 320, 1709
HpyF3I	CTNAG	2 cut(s)	1265, 1631
HpySE526I	ACGT	1 cut(s)	611
Hsp92II	CATG	9 cut(s)	580, 677, 1301, 1324, 1375, 1617, 1621, 1697, 1728
HspAI	GCGC	2 cut(s)	301, 594
KroI	GCCGGC	1 cut(s)	638
KroNI	GCCGGC	1 cut(s)	640
Ksp22I	TGATCA	1 cut(s)	1603
Kzo9I	GATC	5 cut(s)	378, 539, 1509, 1603, 1624
LguI	GCTCTTC	1 cut(s)	204
LmnI	GCTCC	6 cut(s)	26, 1072, 1177, 1223, 1489, 1818
Lsp1109I	GCAGC	3 cut(s)	510, 518, 1819
LweI	GCATC	1 cut(s)	963
MabI	ACCWGGT	1 cut(s)	1903
MaeI	CTAG	3 cut(s)	294, 395, 723
MaeII	ACGT	1 cut(s)	611
MalI	GATC	5 cut(s)	380, 541, 1511, 1605, 1626
MboI	GATC	5 cut(s)	378, 539, 1509, 1603, 1624
MflI	RGATCY	1 cut(s)	539
MhlI	GDGCHC	3 cut(s)	769, 1714, 1815
MluCI	AATT	5 cut(s)	141, 422, 1238, 1389, 1917
MlyI	GAGTC	2 cut(s)	290, 1767
MmeI	TCCRAC	2 cut(s)	1830, 1845
Mph1103I	ATGCAT	2 cut(s)	1615, 1619
MroNI	GCCGGC	1 cut(s)	638
MseI	TTAA	5 cut(s)	165, 180, 1074, 1469, 1893
MslI	CAYNNNNRTG	4 cut(s)	127, 575, 1698, 1740
MspA1I	CMGCKG	2 cut(s)	531, 1845
MspI	CCGG	8 cut(s)	543, 639, 655, 837, 884, 977, 1029, 1636
MspR9I	CCNGG	5 cut(s)	656, 978, 1030, 1048, 1905
Mva1269I	GAATGC	4 cut(s)	898, 955, 1330, 1835
MvaI	CCWGG	2 cut(s)	1048, 1905
MvnI	CGCG	4 cut(s)	204, 303, 483, 596
MwoI	GCNNNNNNNGC	3 cut(s)	298, 320, 1709
NaeI	GCCGGC	1 cut(s)	640
NciI	CCSGG	3 cut(s)	656, 978, 1030
NdeII	GATC	5 cut(s)	378, 539, 1509, 1603, 1624
NgoMIV	GCCGGC	1 cut(s)	638
NlaIII	CATG	9 cut(s)	580, 677, 1301, 1324, 1375, 1617, 1621, 1697, 1728
NlaIV	GGNNCC	8 cut(s)	28, 653, 1068, 1179, 1385, 1485, 1593, 1689
NmeAIII	GCCGAG	1 cut(s)	1457
NsiI	ATGCAT	2 cut(s)	1615, 1619
NspI	RCATGY	3 cut(s)	1375, 1617, 1728
PaeI	GCATGC	1 cut(s)	1617
PceI	AGGCCT	2 cut(s)	632, 1890
PciSI	GCTCTTC	1 cut(s)	204
PctI	GAATGC	4 cut(s)	898, 955, 1330, 1835
PdiI	GCCGGC	1 cut(s)	640
PfeI	GAWTC	2 cut(s)	1256, 1438
PfoI	TCCNGGA	1 cut(s)	654
PkrI	GCNGC	8 cut(s)	80, 482, 500, 530, 533, 598, 1309, 1834
PleI	GAGTC	2 cut(s)	289, 1767
PpsI	GAGTC	2 cut(s)	289, 1767
Ppu21I	YACGTR	1 cut(s)	612
PpuMI	RGGWCCY	1 cut(s)	1384
PshBI	ATTAAT	1 cut(s)	180
PsiI	TTATAA	1 cut(s)	1193
Psp5II	RGGWCCY	1 cut(s)	1384
Psp6I	CCWGG	2 cut(s)	1046, 1903
PspFI	CCCAGC	1 cut(s)	494
PspGI	CCWGG	2 cut(s)	1046, 1903
PspN4I	GGNNCC	8 cut(s)	28, 653, 1068, 1179, 1385, 1485, 1593, 1689
PspPI	GGNCC	3 cut(s)	206, 652, 1384
PspPPI	RGGWCCY	1 cut(s)	1384
PsuI	RGATCY	1 cut(s)	539
RsaI	GTAC	2 cut(s)	608, 614
RsaNI	GTAC	2 cut(s)	607, 613
RseI	CAYNNNNRTG	4 cut(s)	127, 575, 1698, 1740
SalI	GTCGAC	1 cut(s)	731
SapI	GCTCTTC	1 cut(s)	204
SaqAI	TTAA	5 cut(s)	165, 180, 1074, 1469, 1893
SatI	GCNGC	8 cut(s)	79, 481, 499, 529, 532, 597, 1308, 1833
Sau3AI	GATC	5 cut(s)	378, 539, 1509, 1603, 1624
Sau96I	GGNCC	3 cut(s)	206, 652, 1384
SchI	GAGTC	2 cut(s)	290, 1767
ScrFI	CCNGG	5 cut(s)	656, 978, 1030, 1048, 1905
SduI	GDGCHC	3 cut(s)	769, 1714, 1815
SexAI	ACCWGGT	1 cut(s)	1903
SfaNI	GCATC	1 cut(s)	963
SfcI	CTRYAG	1 cut(s)	224
SinI	GGWCC	3 cut(s)	206, 652, 1384
SmiMI	CAYNNNNRTG	4 cut(s)	127, 575, 1698, 1740
SmlI	CTYRAG	1 cut(s)	1154
SmoI	CTYRAG	1 cut(s)	1154
SphI	GCATGC	1 cut(s)	1617
Sse9I	AATT	5 cut(s)	141, 422, 1238, 1389, 1917
SseBI	AGGCCT	2 cut(s)	632, 1890
SspI	AATATT	2 cut(s)	178, 1149
SspMI	CTAG	3 cut(s)	294, 395, 723
StuI	AGGCCT	2 cut(s)	632, 1890
StyD4I	CCNGG	5 cut(s)	654, 976, 1028, 1046, 1903
TaaI	ACNGT	3 cut(s)	559, 1507, 1586
TaiI	ACGT	1 cut(s)	614
TaqI	TCGA	4 cut(s)	732, 1281, 1288, 1771
TasI	AATT	5 cut(s)	141, 422, 1238, 1389, 1917
TatI	WGTACW	1 cut(s)	606
TauI	GCSGC	5 cut(s)	81, 483, 531, 599, 1310
TfiI	GAWTC	2 cut(s)	1256, 1438
Tru1I	TTAA	5 cut(s)	165, 180, 1074, 1469, 1893
Tru9I	TTAA	5 cut(s)	165, 180, 1074, 1469, 1893
TscAI	CASTG	3 cut(s)	192, 198, 1512
TseI	GCWGC	3 cut(s)	498, 531, 1832
TspDTI	ATGAA	6 cut(s)	111, 415, 559, 593, 662, 1337
TspGWI	ACGGA	2 cut(s)	344, 784
TspRI	CASTG	3 cut(s)	192, 198, 1512
VneI	GTGCAC	2 cut(s)	765, 1710
VpaK11BI	GGWCC	3 cut(s)	206, 652, 1384
VspI	ATTAAT	1 cut(s)	180
XbaI	TCTAGA	1 cut(s)	394
XceI	RCATGY	3 cut(s)	1375, 1617, 1728
XmiI	GTMKAC	1 cut(s)	732
XspI	CTAG	3 cut(s)	294, 395, 723
Zsp2I	ATGCAT	2 cut(s)	1615, 1619

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.