Rh4AG290300

TIFY 9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0006870
Physical Location & Seq
Reverse (-)
60388679 .. 60390727
2049 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG290300.1

Sequence Viewer

Length: 597 bp
ATGTACACACCGGTCGCGGCAACCGTCGAGCTCGATTTCTTGGGAGTCGGGACGATGACGATTATGGATCAGCACCGCCACAACCAGCAGCGTGGTGGTGGTGGTGACACCTTCTCTACTGCCTTGCCCAAATCTCCGTTCCAGAAGTTCCTCGAACGCGGAAGAAGCTTCCGAGGAATCCAGGCCCGAGAAGTGATTCCTAATCTTCTCAGTTCCGAAGTTCTGAAATCCGTGATTGCGGCCAGGAAATCCGTGGCTGCGGCTCCGGAGAATTCCCCTGAAAAACCAAAAGCAAAGCCTATGACGATTTTCTACGGCGGAAGCGTCTCTGTTTTCGACGTCACTCCGGACAAGTTTGAGAGCATAGTGAAGCTTGCCATGGAAGAGAAATTCAAAGCTGCTGGAGCTGCAGATCAACCACTGGAGCCAAAACAACAACAGCTGGTGAATGTTGAGGATCCTCTCAATGAAGGTAGGTTCTGTAATATTTCACTTTGCATTTACATGTTAGAGATTTCTTTTTTGACTCTAACTTTAAGGAATTACATTTCGTTGAGAATACTACACTTAGTATTGAATTTAAATACAGTAAAGTAG

Protein Analysis

198

Amino Acids

21.94

Weight (kDa)

8.87

Isoelectric Point (pI)

40.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tify PF06200 96 - 126 6.6e-11 tify domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 342
AccII CGCG 2 cut(s) 17, 159
AccIII TCCGGA 2 cut(s) 265, 346
AciI CCGC 6 cut(s) 17, 76, 159, 239, 260, 318
AclWI GGATC 3 cut(s) 75, 452, 465
AcoI YGGCCR 1 cut(s) 240
AcsI RAATTY 3 cut(s) 271, 389, 577
AcyI GRCGYC 1 cut(s) 339
AfaI GTAC 1 cut(s) 5
AflIII ACRYGT 1 cut(s) 504
AgeI ACCGGT 1 cut(s) 10
AgsI TTSAA 2 cut(s) 394, 577
AjnI CCWGG 2 cut(s) 180, 242
AjuI GAANNNNNNNTTGG 2 cut(s) 122, 154
AluBI AGCT 6 cut(s) 31, 168, 373, 398, 407, 442
AluI AGCT 6 cut(s) 31, 168, 373, 398, 407, 442
Alw21I GWGCWC 1 cut(s) 33
Alw26I GTCTC 1 cut(s) 331
AlwI GGATC 3 cut(s) 75, 452, 465
Ama87I CYCGRG 1 cut(s) 186
Aor13HI TCCGGA 2 cut(s) 265, 346
AoxI GGCC 2 cut(s) 183, 240
ApeKI GCWGC 4 cut(s) 88, 257, 398, 407
ApoI RAATTY 3 cut(s) 271, 389, 577
AsiGI ACCGGT 1 cut(s) 10
Asp700I GAANNNNTTC 1 cut(s) 195
AspS9I GGNCC 1 cut(s) 184
AsuHPI GGTGA 2 cut(s) 116, 457
AvaI CYCGRG 1 cut(s) 186
BamHI GGATCC 1 cut(s) 457
BanII GRGCYC 1 cut(s) 33
Bbv12I GWGCWC 1 cut(s) 33
BbvI GCAGC 4 cut(s) 100, 244, 385, 394
BceAI ACGGC 1 cut(s) 331
BciT130I CCWGG 2 cut(s) 182, 244
BcoDI GTCTC 1 cut(s) 331
BfmI CTRYAG 1 cut(s) 408
BisI GCNGC 7 cut(s) 18, 89, 240, 258, 261, 399, 408
BlsI GCNGC 7 cut(s) 19, 90, 241, 259, 262, 400, 409
Bme1390I CCNGG 2 cut(s) 182, 244
BmeT110I CYCGRG 1 cut(s) 186
BmgT120I GGNCC 1 cut(s) 184
BmiI GGNNCC 3 cut(s) 264, 426, 459
BmrFI CCNGG 2 cut(s) 182, 244
BpmI CTGGAG 2 cut(s) 423, 443
BsaHI GRCGYC 1 cut(s) 339
BsaJI CCNNGG 3 cut(s) 172, 252, 378
BsaWI WCCGGW 3 cut(s) 10, 265, 346
Bse118I RCCGGY 1 cut(s) 10
Bse1I ACTGG 1 cut(s) 426
BseAI TCCGGA 2 cut(s) 265, 346
BseBI CCWGG 2 cut(s) 182, 244
BseDI CCNNGG 3 cut(s) 172, 252, 378
BseMII CTCAG 1 cut(s) 223
BseNI ACTGG 1 cut(s) 426
BseXI GCAGC 4 cut(s) 100, 244, 385, 394
Bsh1236I CGCG 2 cut(s) 17, 159
Bsh1285I CGRYCG 1 cut(s) 15
BshFI GGCC 2 cut(s) 185, 242
BshTI ACCGGT 1 cut(s) 10
BsiEI CGRYCG 1 cut(s) 15
BsiHKAI GWGCWC 1 cut(s) 33
BsiHKCI CYCGRG 1 cut(s) 186
BsiSI CCGG 3 cut(s) 11, 266, 347
BslFI GGGAC 1 cut(s) 64
BsmAI GTCTC 1 cut(s) 331
BsmBI CGTCTC 1 cut(s) 331
BsmFI GGGAC 1 cut(s) 64
BsnI GGCC 2 cut(s) 185, 242
BsoBI CYCGRG 1 cut(s) 186
Bsp1286I GDGCHC 1 cut(s) 33
Bsp13I TCCGGA 2 cut(s) 265, 346
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 3 cut(s) 67, 412, 457
Bsp19I CCATGG 1 cut(s) 378
BspACI CCGC 6 cut(s) 17, 76, 159, 239, 260, 318
BspANI GGCC 2 cut(s) 185, 242
BspCNI CTCAG 1 cut(s) 222
BspEI TCCGGA 2 cut(s) 265, 346
BspFNI CGCG 2 cut(s) 17, 159
BspLI GGNNCC 3 cut(s) 264, 426, 459
BspMAI CTGCAG 1 cut(s) 412
BspPI GGATC 3 cut(s) 75, 452, 465
BsrFI RCCGGY 1 cut(s) 10
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 1 cut(s) 426
BssAI RCCGGY 1 cut(s) 10
BssECI CCNNGG 3 cut(s) 172, 252, 378
BssMI GATC 3 cut(s) 67, 412, 457
BssNI GRCGYC 1 cut(s) 339
BssT1I CCWWGG 1 cut(s) 378
Bst2UI CCWGG 2 cut(s) 182, 244
Bst4CI ACNGT 2 cut(s) 25, 589
Bst6I CTCTTC 1 cut(s) 378
BstACI GRCGYC 1 cut(s) 339
BstAUI TGTACA 1 cut(s) 3
BstC8I GCNNGC 1 cut(s) 375
BstDEI CTNAG 2 cut(s) 209, 568
BstDSI CCRYGG 2 cut(s) 252, 378
BstFNI CGCG 2 cut(s) 17, 159
BstKTI GATC 3 cut(s) 70, 415, 460
BstMAI GTCTC 1 cut(s) 331
BstMBI GATC 3 cut(s) 67, 412, 457
BstMCI CGRYCG 1 cut(s) 15
BstMWI GCNNNNNNNGC 3 cut(s) 165, 404, 407
BstNI CCWGG 2 cut(s) 182, 244
BstNSI RCATGY 1 cut(s) 508
BstSCI CCNGG 2 cut(s) 180, 242
BstSFI CTRYAG 1 cut(s) 408
BstUI CGCG 2 cut(s) 17, 159
BstV1I GCAGC 4 cut(s) 100, 244, 385, 394
BstX2I RGATCY 1 cut(s) 457
BstXI CCANNNNNNTGG 1 cut(s) 92
BstYI RGATCY 1 cut(s) 457
BsuRI GGCC 2 cut(s) 185, 242
BtgI CCRYGG 2 cut(s) 252, 378
BtsIMutI CAGTG 1 cut(s) 419
Cac8I GCNNGC 1 cut(s) 375
Cfr10I RCCGGY 1 cut(s) 10
Cfr13I GGNCC 1 cut(s) 184
CseI GACGC 1 cut(s) 313
Csp6I GTAC 1 cut(s) 4
CspAI ACCGGT 1 cut(s) 10
CviAII CATG 2 cut(s) 379, 505
CviQI GTAC 1 cut(s) 4
DdeI CTNAG 2 cut(s) 209, 568
DpnI GATC 3 cut(s) 69, 414, 459
DpnII GATC 3 cut(s) 67, 412, 457
DraI TTTAAA 1 cut(s) 582
EaeI YGGCCR 1 cut(s) 240
Eam1104I CTCTTC 1 cut(s) 378
EarI CTCTTC 1 cut(s) 378
EciI GGCGGA 1 cut(s) 333
Ecl136II GAGCTC 1 cut(s) 31
Eco130I CCWWGG 1 cut(s) 378
Eco24I GRGCYC 1 cut(s) 33
Eco53kI GAGCTC 1 cut(s) 31
Eco88I CYCGRG 1 cut(s) 186
EcoICRI GAGCTC 1 cut(s) 31
EcoRI GAATTC 1 cut(s) 271
EcoRII CCWGG 2 cut(s) 180, 242
EcoT14I CCWWGG 1 cut(s) 378
EcoT38I GRGCYC 1 cut(s) 33
ErhI CCWWGG 1 cut(s) 378
Esp3I CGTCTC 1 cut(s) 331
FaeI CATG 2 cut(s) 382, 508
FaiI YATR 5 cut(s) 65, 302, 365, 380, 506
FaqI GGGAC 1 cut(s) 64
FatI CATG 2 cut(s) 378, 504
Fnu4HI GCNGC 7 cut(s) 18, 89, 240, 258, 261, 399, 408
FriOI GRGCYC 1 cut(s) 33
Fsp4HI GCNGC 7 cut(s) 18, 89, 240, 258, 261, 399, 408
GluI GCNGC 7 cut(s) 18, 89, 240, 258, 261, 399, 408
GsuI CTGGAG 2 cut(s) 423, 443
HaeIII GGCC 2 cut(s) 185, 242
HapII CCGG 3 cut(s) 11, 266, 347
HgaI GACGC 1 cut(s) 313
Hin1I GRCGYC 1 cut(s) 339
Hin1II CATG 2 cut(s) 382, 508
HindIII AAGCTT 2 cut(s) 166, 371
HinfI GANTC 4 cut(s) 45, 177, 196, 526
HpaII CCGG 3 cut(s) 11, 266, 347
HphI GGTGA 2 cut(s) 116, 457
Hpy166II GTNNAC 1 cut(s) 6
Hpy188I TCNGA 3 cut(s) 173, 217, 225
Hpy188III TCNNGA 4 cut(s) 49, 142, 266, 347
Hpy8I GTNNAC 1 cut(s) 6
Hpy99I CGWCG 2 cut(s) 29, 341
HpyAV CCTTC 2 cut(s) 121, 464
HpyCH4III ACNGT 2 cut(s) 25, 589
HpyCH4IV ACGT 1 cut(s) 339
HpyCH4V TGCA 2 cut(s) 410, 498
HpyF10VI GCNNNNNNNGC 3 cut(s) 165, 404, 407
HpyF3I CTNAG 2 cut(s) 209, 568
HpySE526I ACGT 1 cut(s) 339
Hsp92I GRCGYC 1 cut(s) 339
Hsp92II CATG 2 cut(s) 382, 508
Kpn2I TCCGGA 2 cut(s) 265, 346
Kzo9I GATC 3 cut(s) 67, 412, 457
LmnI GCTCC 3 cut(s) 268, 404, 424
Lsp1109I GCAGC 4 cut(s) 100, 244, 385, 394
MaeII ACGT 1 cut(s) 339
MaeIII GTNAC 2 cut(s) 104, 340
MalI GATC 3 cut(s) 69, 414, 459
MboI GATC 3 cut(s) 67, 412, 457
MboII GAAGA 3 cut(s) 174, 197, 395
MflI RGATCY 1 cut(s) 457
MhlI GDGCHC 1 cut(s) 33
MluCI AATT 4 cut(s) 271, 389, 541, 577
MlyI GAGTC 2 cut(s) 54, 520
MnlI CCTC 4 cut(s) 161, 167, 448, 471
MroI TCCGGA 2 cut(s) 265, 346
MroXI GAANNNNTTC 1 cut(s) 195
MseI TTAA 2 cut(s) 536, 581
MslI CAYNNNNRTG 1 cut(s) 503
MspA1I CMGCKG 1 cut(s) 442
MspI CCGG 3 cut(s) 11, 266, 347
MspR9I CCNGG 2 cut(s) 182, 244
MvaI CCWGG 2 cut(s) 182, 244
MvnI CGCG 2 cut(s) 17, 159
MwoI GCNNNNNNNGC 3 cut(s) 165, 404, 407
NcoI CCATGG 1 cut(s) 378
NdeII GATC 3 cut(s) 67, 412, 457
NlaIII CATG 2 cut(s) 382, 508
NlaIV GGNNCC 3 cut(s) 264, 426, 459
NmuCI GTSAC 2 cut(s) 104, 340
NspI RCATGY 1 cut(s) 508
PciI ACATGT 1 cut(s) 504
PcsI WCGNNNNNNNCGW 2 cut(s) 21, 321
PdmI GAANNNNTTC 1 cut(s) 195
PfeI GAWTC 2 cut(s) 177, 196
PinAI ACCGGT 1 cut(s) 10
PkrI GCNGC 7 cut(s) 19, 90, 241, 259, 262, 400, 409
PleI GAGTC 2 cut(s) 53, 520
PpsI GAGTC 2 cut(s) 53, 520
PscI ACATGT 1 cut(s) 504
Psp124BI GAGCTC 1 cut(s) 33
Psp6I CCWGG 2 cut(s) 180, 242
PspGI CCWGG 2 cut(s) 180, 242
PspN4I GGNNCC 3 cut(s) 264, 426, 459
PspPI GGNCC 1 cut(s) 184
PstI CTGCAG 1 cut(s) 412
PsuI RGATCY 1 cut(s) 457
PvuII CAGCTG 1 cut(s) 442
RsaI GTAC 1 cut(s) 5
RsaNI GTAC 1 cut(s) 4
RseI CAYNNNNRTG 1 cut(s) 503
SacI GAGCTC 1 cut(s) 33
SaqAI TTAA 2 cut(s) 536, 581
SatI GCNGC 7 cut(s) 18, 89, 240, 258, 261, 399, 408
Sau3AI GATC 3 cut(s) 67, 412, 457
Sau96I GGNCC 1 cut(s) 184
SchI GAGTC 2 cut(s) 54, 520
ScrFI CCNGG 2 cut(s) 182, 244
SduI GDGCHC 1 cut(s) 33
SfcI CTRYAG 1 cut(s) 408
SmiI ATTTAAAT 1 cut(s) 582
SmiMI CAYNNNNRTG 1 cut(s) 503
Sse9I AATT 4 cut(s) 271, 389, 541, 577
SsiI CCGC 6 cut(s) 17, 76, 159, 239, 260, 318
SspI AATATT 1 cut(s) 487
SstI GAGCTC 1 cut(s) 33
StyD4I CCNGG 2 cut(s) 180, 242
StyI CCWWGG 1 cut(s) 378
SwaI ATTTAAAT 1 cut(s) 582
TaaI ACNGT 2 cut(s) 25, 589
TaiI ACGT 1 cut(s) 342
TaqI TCGA 4 cut(s) 27, 33, 153, 336
TasI AATT 4 cut(s) 271, 389, 541, 577
TatI WGTACW 1 cut(s) 3
TauI GCSGC 3 cut(s) 20, 242, 263
TfiI GAWTC 2 cut(s) 177, 196
Tru1I TTAA 2 cut(s) 536, 581
Tru9I TTAA 2 cut(s) 536, 581
TscAI CASTG 1 cut(s) 426
TseFI GTSAC 2 cut(s) 104, 340
TseI GCWGC 4 cut(s) 88, 257, 398, 407
Tsp45I GTSAC 2 cut(s) 104, 340
TspDTI ATGAA 1 cut(s) 483
TspGWI ACGGA 3 cut(s) 126, 220, 241
TspRI CASTG 1 cut(s) 426
XapI RAATTY 3 cut(s) 271, 389, 577
XceI RCATGY 1 cut(s) 508
XcmI CCANNNNNNNNNTGG 2 cut(s) 92, 250
XmnI GAANNNNTTC 1 cut(s) 195
ZraI GACGTC 1 cut(s) 340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.