Rh5BG519700

NifU-like protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0017763
Physical Location & Seq
Reverse (-)
82182948 .. 82189432
6485 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG519700.1

Sequence Viewer

Length: 423 bp
ATGGCTGCCGAGCAGAGGACAATGGTCACCCTCATGACCATCAATGATGGTGGAAACGTCGACATTGTGTCCGTCGAAGACGGCGTCGTTTCTCTCAAGCTCCAAGGGGCTTGTGGAAGCTGTCCTAGCTCAACAACCACCATGAAAATGGGGATTGAACGAGTCCTTAAAGAAAAGTGTGCAGATGCACTCAAAGATATTCAACAAGTCTTTGATGAAGAAAACAGAGAGACCACAGTTGAGGTTCTCAAGGGTCCAAATGCACCATTCTTAATGTCTGGTTGCAAAATAGCTGATGGAGTCGGTACCATGCTGGTAACGAGTGTTGGAAGCAATACAGGATGGGGATTGTTGATGGCAAGTATCTCTGAGGATACCGGTGAAGAAGCTCCTTTACAGATACTCTGCGAGATTAGAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005198 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NifU

Protein Analysis

140

Amino Acids

14.84

Weight (kDa)

4.39

Isoelectric Point (pI)

38.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NifU PF01106 14 - 62 2.3e-18 NifU-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017763)

Species Orthologous Gene IDs
pyrus_communis pycom16g20660
rosa_chinensis RchiOBHm_Chr2g0124791 RchiOBHm_Chr2g0133041
rosa_laevigata RLG00000014296
rosa_roxburghii Rroxscaffold_1G00004640 Rroxscaffold_6G00396430
rosa_samantha Rh5BG519700 Rh5CG543700 Rh7BG392600 Rh7DG118800 Rh7DG442200
rosa_wichuraiana Rw7G024550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 305
AccB1I GGYRCC 1 cut(s) 305
AccI GTMKAC 1 cut(s) 60
AcsI RAATTY 1 cut(s) 417
AcyI GRCGYC 1 cut(s) 84
AfaI GTAC 1 cut(s) 307
AfiI CCNNNNNNNGG 1 cut(s) 15
AgeI ACCGGT 1 cut(s) 377
AgsI TTSAA 2 cut(s) 158, 203
AhdI GACNNNNNGTC 1 cut(s) 67
AluBI AGCT 5 cut(s) 100, 120, 129, 293, 389
AluI AGCT 5 cut(s) 100, 120, 129, 293, 389
Alw26I GTCTC 1 cut(s) 224
ApeKI GCWGC 1 cut(s) 5
ApoI RAATTY 1 cut(s) 417
ArsI GACNNNNNNTTYG 2 cut(s) 69, 101
AsiGI ACCGGT 1 cut(s) 377
Asp718I GGTACC 1 cut(s) 305
AspS9I GGNCC 1 cut(s) 254
AsuHPI GGTGA 2 cut(s) 19, 392
AvaII GGWCC 1 cut(s) 254
BanI GGYRCC 1 cut(s) 305
BarI GAAGNNNNNNTAC 2 cut(s) 378, 410
BbsI GAAGAC 1 cut(s) 84
BccI CCATC 5 cut(s) 41, 47, 290, 336, 349
BceAI ACGGC 1 cut(s) 97
BciVI GTATCC 1 cut(s) 367
BcoDI GTCTC 1 cut(s) 224
BfaI CTAG 1 cut(s) 126
BfuI GTATCC 1 cut(s) 367
BisI GCNGC 1 cut(s) 6
BlsI GCNGC 1 cut(s) 7
Bme18I GGWCC 1 cut(s) 254
BmeRI GACNNNNNGTC 1 cut(s) 67
BmgT120I GGNCC 1 cut(s) 254
BmiI GGNNCC 2 cut(s) 255, 307
BmsI GCATC 1 cut(s) 175
BoxI GACNNNNGTC 1 cut(s) 23
BpiI GAAGAC 1 cut(s) 84
BpuEI CTTGAG 2 cut(s) 80, 233
BsaHI GRCGYC 1 cut(s) 84
BsaI GGTCTC 1 cut(s) 224
BsaJI CCNNGG 1 cut(s) 103
BsaWI WCCGGW 1 cut(s) 377
Bsc4I CCNNNNNNNGG 1 cut(s) 15
Bse118I RCCGGY 1 cut(s) 377
BseDI CCNNGG 1 cut(s) 103
BseGI GGATG 1 cut(s) 347
BseLI CCNNNNNNNGG 1 cut(s) 15
BseMII CTCAG 1 cut(s) 360
BsgI GTGCAG 1 cut(s) 201
BshNI GGYRCC 1 cut(s) 305
BshTI ACCGGT 1 cut(s) 377
BsiSI CCGG 1 cut(s) 378
BslI CCNNNNNNNGG 1 cut(s) 15
BsmAI GTCTC 1 cut(s) 224
Bso31I GGTCTC 1 cut(s) 224
BspCNI CTCAG 1 cut(s) 361
BspHI TCATGA 1 cut(s) 33
BspLI GGNNCC 2 cut(s) 255, 307
BspT107I GGYRCC 1 cut(s) 305
BspTNI GGTCTC 1 cut(s) 224
BsrFI RCCGGY 1 cut(s) 377
BssAI RCCGGY 1 cut(s) 377
BssECI CCNNGG 1 cut(s) 103
BssNI GRCGYC 1 cut(s) 84
BssT1I CCWWGG 1 cut(s) 103
Bst4CI ACNGT 1 cut(s) 238
BstACI GRCGYC 1 cut(s) 84
BstDEI CTNAG 1 cut(s) 369
BstEII GGTNACC 1 cut(s) 25
BstF5I GGATG 1 cut(s) 347
BstMAI GTCTC 1 cut(s) 224
BstMWI GCNNNNNNNGC 1 cut(s) 126
BstPAI GACNNNNGTC 1 cut(s) 23
BstPI GGTNACC 1 cut(s) 25
BstV2I GAAGAC 1 cut(s) 84
BstXI CCANNNNNNTGG 1 cut(s) 148
BsuI GTATCC 1 cut(s) 367
BtsCI GGATG 1 cut(s) 347
CciI TCATGA 1 cut(s) 33
Cfr10I RCCGGY 1 cut(s) 377
Cfr13I GGNCC 1 cut(s) 254
CseI GACGC 1 cut(s) 73
Csp6I GTAC 1 cut(s) 306
CspAI ACCGGT 1 cut(s) 377
CspCI CAANNNNNGTGG 2 cut(s) 31, 66
CviAII CATG 3 cut(s) 34, 142, 310
CviJI RGCY 7 cut(s) 5, 100, 110, 120, 129, 293, 389
CviKI_1 RGCY 7 cut(s) 5, 100, 110, 120, 129, 293, 389
CviQI GTAC 1 cut(s) 306
DdeI CTNAG 1 cut(s) 369
DriI GACNNNNNGTC 1 cut(s) 67
Eam1105I GACNNNNNGTC 1 cut(s) 67
Eco130I CCWWGG 1 cut(s) 103
Eco31I GGTCTC 1 cut(s) 224
Eco47I GGWCC 1 cut(s) 254
Eco91I GGTNACC 1 cut(s) 25
EcoO65I GGTNACC 1 cut(s) 25
EcoT14I CCWWGG 1 cut(s) 103
ErhI CCWWGG 1 cut(s) 103
FaeI CATG 3 cut(s) 37, 145, 313
FaiI YATR 3 cut(s) 35, 143, 311
FatI CATG 3 cut(s) 33, 141, 309
FblI GTMKAC 1 cut(s) 60
Fnu4HI GCNGC 1 cut(s) 6
FokI GGATG 1 cut(s) 354
Fsp4HI GCNGC 1 cut(s) 6
FspBI CTAG 1 cut(s) 126
GluI GCNGC 1 cut(s) 6
HapII CCGG 1 cut(s) 378
HgaI GACGC 1 cut(s) 73
Hin1I GRCGYC 1 cut(s) 84
Hin1II CATG 3 cut(s) 37, 145, 313
HincII GTYRAC 1 cut(s) 61
HindII GTYRAC 1 cut(s) 61
HinfI GANTC 2 cut(s) 162, 300
HpaII CCGG 1 cut(s) 378
HphI GGTGA 2 cut(s) 19, 392
Hpy166II GTNNAC 1 cut(s) 61
Hpy188I TCNGA 1 cut(s) 370
Hpy188III TCNNGA 1 cut(s) 34
Hpy8I GTNNAC 1 cut(s) 61
Hpy99I CGWCG 3 cut(s) 62, 77, 89
HpyCH4III ACNGT 1 cut(s) 238
HpyCH4IV ACGT 1 cut(s) 57
HpyCH4V TGCA 4 cut(s) 182, 188, 263, 285
HpyF10VI GCNNNNNNNGC 1 cut(s) 126
HpyF3I CTNAG 1 cut(s) 369
HpySE526I ACGT 1 cut(s) 57
Hsp92I GRCGYC 1 cut(s) 84
Hsp92II CATG 3 cut(s) 37, 145, 313
KpnI GGTACC 1 cut(s) 309
LmnI GCTCC 2 cut(s) 105, 394
LpnPI CCDG 4 cut(s) 264, 299, 324, 391
LweI GCATC 1 cut(s) 175
MaeI CTAG 1 cut(s) 126
MaeII ACGT 1 cut(s) 57
MaeIII GTNAC 2 cut(s) 25, 316
MboII GAAGA 3 cut(s) 89, 230, 395
MluCI AATT 1 cut(s) 417
MlyI GAGTC 2 cut(s) 171, 309
MmeI TCCRAC 1 cut(s) 307
MnlI CCTC 4 cut(s) 9, 41, 235, 364
MseI TTAA 2 cut(s) 168, 272
MslI CAYNNNNRTG 2 cut(s) 32, 146
MspI CCGG 1 cut(s) 378
MwoI GCNNNNNNNGC 1 cut(s) 126
NlaIII CATG 3 cut(s) 37, 145, 313
NlaIV GGNNCC 2 cut(s) 255, 307
NmeAIII GCCGAG 1 cut(s) 34
NmuCI GTSAC 1 cut(s) 25
PagI TCATGA 1 cut(s) 33
PcsI WCGNNNNNNNCGW 1 cut(s) 81
PflFI GACNNNGTC 1 cut(s) 83
PinAI ACCGGT 1 cut(s) 377
PkrI GCNGC 1 cut(s) 7
PleI GAGTC 2 cut(s) 170, 308
PpsI GAGTC 2 cut(s) 170, 308
PshAI GACNNNNGTC 1 cut(s) 23
PspEI GGTNACC 1 cut(s) 25
PspN4I GGNNCC 2 cut(s) 255, 307
PspPI GGNCC 1 cut(s) 254
PsyI GACNNNGTC 1 cut(s) 83
RsaI GTAC 1 cut(s) 307
RsaNI GTAC 1 cut(s) 306
RseI CAYNNNNRTG 2 cut(s) 32, 146
SalI GTCGAC 1 cut(s) 59
SaqAI TTAA 2 cut(s) 168, 272
SatI GCNGC 1 cut(s) 6
Sau96I GGNCC 1 cut(s) 254
SchI GAGTC 2 cut(s) 171, 309
SetI ASST 7 cut(s) 60, 102, 122, 131, 246, 295, 391
SfaNI GCATC 1 cut(s) 175
SinI GGWCC 1 cut(s) 254
SmiMI CAYNNNNRTG 2 cut(s) 32, 146
SmlI CTYRAG 2 cut(s) 95, 248
SmoI CTYRAG 2 cut(s) 95, 248
Sse9I AATT 1 cut(s) 417
SspMI CTAG 1 cut(s) 126
StyI CCWWGG 1 cut(s) 103
TaaI ACNGT 1 cut(s) 238
TaiI ACGT 1 cut(s) 60
TaqI TCGA 2 cut(s) 60, 75
TasI AATT 1 cut(s) 417
Tru1I TTAA 2 cut(s) 168, 272
Tru9I TTAA 2 cut(s) 168, 272
TseFI GTSAC 1 cut(s) 25
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 1 cut(s) 25
TspDTI ATGAA 2 cut(s) 158, 231
TspGWI ACGGA 1 cut(s) 61
Tth111I GACNNNGTC 1 cut(s) 83
VpaK11BI GGWCC 1 cut(s) 254
XapI RAATTY 1 cut(s) 417
XcmI CCANNNNNNNNNTGG 2 cut(s) 110, 145
XmiI GTMKAC 1 cut(s) 60
XspI CTAG 1 cut(s) 126
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.