Rh7DG442200

NifU-like protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0017763
Physical Location & Seq
Forward (+)
62768138 .. 62770391
2254 bp
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UTR
Exon/CDS
Intron
Rh7DG442200.1

Sequence Viewer

Length: 294 bp
ATGGTTTCCGCGAGCACTAAGCCTGACAAAACGCCCAACACTCTTACTCTGACATCCAAGCAACAAAAGCTCATCAACGATGGTGGAGACGTCGACATTGTGTCCGTCGAAGACGGCGTCATTTCTCTCAAGCTCCAAGGGGCTTGTGGAAGCTGTCCTAGCTCAACAACCACCATGAAAATAGGGATTGAACGAGTGCTTAAAGAAAAGTTTGCAGATGCACTCAAGGATATTCAACAAGTCTTTGATGAAGAAAACAGAGAGACCACAGTTGAGGCAGTCTCGACATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005198 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NifU

Protein Analysis

97

Amino Acids

10.47

Weight (kDa)

4.71

Isoelectric Point (pI)

31.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NifU PF01106 22 - 73 1e-18 NifU-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017763)

Species Orthologous Gene IDs
pyrus_communis pycom16g20660
rosa_chinensis RchiOBHm_Chr2g0124791 RchiOBHm_Chr2g0133041
rosa_laevigata RLG00000014296
rosa_roxburghii Rroxscaffold_1G00004640 Rroxscaffold_6G00396430
rosa_samantha Rh5BG519700 Rh5CG543700 Rh7BG392600 Rh7DG118800 Rh7DG442200
rosa_wichuraiana Rw7G024550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 93
AccI GTMKAC 1 cut(s) 93
AccII CGCG 1 cut(s) 11
AciI CCGC 1 cut(s) 9
AcyI GRCGYC 2 cut(s) 90, 117
AgsI TTSAA 2 cut(s) 191, 236
AhdI GACNNNNNGTC 1 cut(s) 100
AluBI AGCT 4 cut(s) 70, 133, 153, 162
AluI AGCT 4 cut(s) 70, 133, 153, 162
Alw21I GWGCWC 1 cut(s) 17
Alw26I GTCTC 3 cut(s) 81, 257, 286
ArsI GACNNNNNNTTYG 2 cut(s) 102, 134
BbsI GAAGAC 1 cut(s) 117
Bbv12I GWGCWC 1 cut(s) 17
BccI CCATC 1 cut(s) 74
BceAI ACGGC 1 cut(s) 130
BcoDI GTCTC 3 cut(s) 81, 257, 286
BfaI CTAG 1 cut(s) 159
BmeRI GACNNNNNGTC 1 cut(s) 100
BmsI GCATC 1 cut(s) 208
BpiI GAAGAC 1 cut(s) 117
BplI GAGNNNNNCTC 1 cut(s) 266
BpuEI CTTGAG 2 cut(s) 113, 209
BsaHI GRCGYC 2 cut(s) 90, 117
BsaI GGTCTC 1 cut(s) 257
BsaJI CCNNGG 1 cut(s) 136
BseDI CCNNGG 1 cut(s) 136
BseGI GGATG 1 cut(s) 53
Bsh1236I CGCG 1 cut(s) 11
BsiHKAI GWGCWC 1 cut(s) 17
BsmAI GTCTC 3 cut(s) 81, 257, 286
BsmBI CGTCTC 1 cut(s) 81
Bso31I GGTCTC 1 cut(s) 257
Bsp1286I GDGCHC 1 cut(s) 17
BspACI CCGC 1 cut(s) 9
BspFNI CGCG 1 cut(s) 11
BspTNI GGTCTC 1 cut(s) 257
BssECI CCNNGG 1 cut(s) 136
BssNI GRCGYC 2 cut(s) 90, 117
BssT1I CCWWGG 1 cut(s) 136
Bst4CI ACNGT 1 cut(s) 271
BstACI GRCGYC 2 cut(s) 90, 117
BstC8I GCNNGC 1 cut(s) 13
BstDEI CTNAG 1 cut(s) 18
BstF5I GGATG 1 cut(s) 53
BstFNI CGCG 1 cut(s) 11
BstMAI GTCTC 3 cut(s) 81, 257, 286
BstMWI GCNNNNNNNGC 2 cut(s) 67, 159
BstUI CGCG 1 cut(s) 11
BstV2I GAAGAC 1 cut(s) 117
BtsCI GGATG 1 cut(s) 53
Cac8I GCNNGC 1 cut(s) 13
CseI GACGC 1 cut(s) 106
CspCI CAANNNNNGTGG 2 cut(s) 64, 99
CviAII CATG 1 cut(s) 175
CviJI RGCY 6 cut(s) 22, 70, 133, 143, 153, 162
CviKI_1 RGCY 6 cut(s) 22, 70, 133, 143, 153, 162
DdeI CTNAG 1 cut(s) 18
DriI GACNNNNNGTC 1 cut(s) 100
Eam1105I GACNNNNNGTC 1 cut(s) 100
Eco130I CCWWGG 1 cut(s) 136
Eco31I GGTCTC 1 cut(s) 257
EcoT14I CCWWGG 1 cut(s) 136
ErhI CCWWGG 1 cut(s) 136
Esp3I CGTCTC 1 cut(s) 81
FaeI CATG 1 cut(s) 178
FaiI YATR 2 cut(s) 176, 289
FatI CATG 1 cut(s) 174
FblI GTMKAC 1 cut(s) 93
FokI GGATG 1 cut(s) 40
FspBI CTAG 1 cut(s) 159
HgaI GACGC 1 cut(s) 106
Hin1I GRCGYC 2 cut(s) 90, 117
Hin1II CATG 1 cut(s) 178
HincII GTYRAC 1 cut(s) 94
HindII GTYRAC 1 cut(s) 94
Hpy166II GTNNAC 1 cut(s) 94
Hpy188I TCNGA 1 cut(s) 51
Hpy188III TCNNGA 1 cut(s) 283
Hpy8I GTNNAC 1 cut(s) 94
Hpy99I CGWCG 2 cut(s) 95, 110
HpyCH4III ACNGT 1 cut(s) 271
HpyCH4IV ACGT 1 cut(s) 90
HpyCH4V TGCA 2 cut(s) 215, 221
HpyF10VI GCNNNNNNNGC 2 cut(s) 67, 159
HpyF3I CTNAG 1 cut(s) 18
HpySE526I ACGT 1 cut(s) 90
Hsp92I GRCGYC 2 cut(s) 90, 117
Hsp92II CATG 1 cut(s) 178
LmnI GCTCC 1 cut(s) 138
LpnPI CCDG 1 cut(s) 36
LweI GCATC 1 cut(s) 208
MaeI CTAG 1 cut(s) 159
MaeII ACGT 1 cut(s) 90
MboII GAAGA 2 cut(s) 122, 263
MhlI GDGCHC 1 cut(s) 17
MnlI CCTC 1 cut(s) 268
MseI TTAA 1 cut(s) 201
MvnI CGCG 1 cut(s) 11
MwoI GCNNNNNNNGC 2 cut(s) 67, 159
NlaIII CATG 1 cut(s) 178
PcsI WCGNNNNNNNCGW 1 cut(s) 114
PflFI GACNNNGTC 1 cut(s) 116
PsyI GACNNNGTC 1 cut(s) 116
SalI GTCGAC 1 cut(s) 92
SaqAI TTAA 1 cut(s) 201
SduI GDGCHC 1 cut(s) 17
SetI ASST 5 cut(s) 72, 93, 135, 155, 164
SfaNI GCATC 1 cut(s) 208
SmlI CTYRAG 2 cut(s) 128, 224
SmoI CTYRAG 2 cut(s) 128, 224
SsiI CCGC 1 cut(s) 9
SspMI CTAG 1 cut(s) 159
StyI CCWWGG 1 cut(s) 136
TaaI ACNGT 1 cut(s) 271
TaiI ACGT 1 cut(s) 93
TaqI TCGA 3 cut(s) 93, 108, 284
Tru1I TTAA 1 cut(s) 201
Tru9I TTAA 1 cut(s) 201
TspDTI ATGAA 2 cut(s) 191, 264
TspGWI ACGGA 1 cut(s) 94
Tth111I GACNNNGTC 1 cut(s) 116
XcmI CCANNNNNNNNNTGG 1 cut(s) 143
XmiI GTMKAC 1 cut(s) 93
XspI CTAG 1 cut(s) 159
ZraI GACGTC 1 cut(s) 91
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.