Rh5CG543700

NifU-like protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0017763
Physical Location & Seq
Reverse (-)
75881994 .. 75893361
11368 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG543700.1

Sequence Viewer

Length: 609 bp
ATGTGTGTTGTTATGTTGGAAGAAGAAGAAGGAGCGTCTTTTGAAAAGGCTCAGGCAGAGACTGAAGGAAAGATTGTTGAAGCCAAGAAGCCTATTGTTGTGAAGTACGGTCTCAACCATGTCACCTACCTCATTGAACAGACCATCAATGATGGTGGAAACGTCGACATTGTGTCCGTCGAAGACGGCGTCGTTTCTCTCAAGCTCCAAGGGGCTTGTGGAAGCTGTCCTAGCTCAACAACCACCATGAAAATGGGGATTGAACGAGTCCTTAAAGAAAAGTGTGCAGATGCACTCAAAGATATTCAACAAGTCTTTGATGAAGAAAACAGAGAGACCACAGTTGAGAATGATGCTGTATTACCAGAGCTACTGGCCAAGTTAATTAGCATTTTTGGTTGTGCTCAATATGTCTTCGAATTGTCGTTAGAGAAGATTTATGAAGTCTGCCCCTACCTGATTAGATATCCTAGCTCCGCCACTACTTGCAAGAAATGCCCGCCCATTCAGCTCGCCAGTAGCACCGCCCGCACCAGATTCACTAGCGAACCTGATTCAAGCTCCACCAGCGAGGTTTTTTTAGCGTCTCCGATGTCAAAGCGACAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005198 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NifU

Protein Analysis

202

Amino Acids

22.18

Weight (kDa)

4.8

Isoelectric Point (pI)

45.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NifU PF01106 50 - 96 4.9e-18 NifU-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017763)

Species Orthologous Gene IDs
pyrus_communis pycom16g20660
rosa_chinensis RchiOBHm_Chr2g0124791 RchiOBHm_Chr2g0133041
rosa_laevigata RLG00000014296
rosa_roxburghii Rroxscaffold_1G00004640 Rroxscaffold_6G00396430
rosa_samantha Rh5BG519700 Rh5CG543700 Rh7BG392600 Rh7DG118800 Rh7DG442200
rosa_wichuraiana Rw7G024550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 165
AciI CCGC 4 cut(s) 477, 500, 525, 529
AcoI YGGCCR 1 cut(s) 375
AcuI CTGAAG 1 cut(s) 84
AcyI GRCGYC 1 cut(s) 189
AfaI GTAC 1 cut(s) 107
AgsI TTSAA 6 cut(s) 44, 80, 137, 263, 308, 558
AhdI GACNNNNNGTC 1 cut(s) 172
AluBI AGCT 7 cut(s) 205, 225, 234, 370, 474, 511, 561
AluI AGCT 7 cut(s) 205, 225, 234, 370, 474, 511, 561
Alw21I GWGCWC 1 cut(s) 406
Alw26I GTCTC 4 cut(s) 53, 116, 329, 591
AlwNI CAGNNNCTG 1 cut(s) 62
AoxI GGCC 1 cut(s) 375
ArsI GACNNNNNNTTYG 2 cut(s) 174, 206
AsuHPI GGTGA 1 cut(s) 115
AsuII TTCGAA 1 cut(s) 417
BalI TGGCCA 1 cut(s) 377
BbsI GAAGAC 2 cut(s) 189, 406
Bbv12I GWGCWC 1 cut(s) 406
BccI CCATC 2 cut(s) 146, 152
BceAI ACGGC 1 cut(s) 202
BcoDI GTCTC 4 cut(s) 53, 116, 329, 591
BfaI CTAG 3 cut(s) 231, 471, 543
BmeRI GACNNNNNGTC 1 cut(s) 172
BmsI GCATC 2 cut(s) 280, 343
BpiI GAAGAC 2 cut(s) 189, 406
Bpu10I CCTNAGC 1 cut(s) 51
Bpu14I TTCGAA 1 cut(s) 417
BpuEI CTTGAG 1 cut(s) 185
BsaHI GRCGYC 1 cut(s) 189
BsaI GGTCTC 2 cut(s) 116, 329
BsaJI CCNNGG 1 cut(s) 208
Bse1I ACTGG 2 cut(s) 378, 516
BseDI CCNNGG 1 cut(s) 208
BseMII CTCAG 1 cut(s) 65
BseNI ACTGG 2 cut(s) 378, 516
BsgI GTGCAG 1 cut(s) 306
BshFI GGCC 1 cut(s) 377
BsiHKAI GWGCWC 1 cut(s) 406
BsmAI GTCTC 4 cut(s) 53, 116, 329, 591
BsmBI CGTCTC 1 cut(s) 591
BsnI GGCC 1 cut(s) 377
Bso31I GGTCTC 2 cut(s) 116, 329
Bsp119I TTCGAA 1 cut(s) 417
Bsp1286I GDGCHC 1 cut(s) 406
BspACI CCGC 4 cut(s) 477, 500, 525, 529
BspANI GGCC 1 cut(s) 377
BspCNI CTCAG 1 cut(s) 64
BspT104I TTCGAA 1 cut(s) 417
BspTNI GGTCTC 2 cut(s) 116, 329
BsrI ACTGG 2 cut(s) 378, 516
BssECI CCNNGG 1 cut(s) 208
BssNI GRCGYC 1 cut(s) 189
BssT1I CCWWGG 1 cut(s) 208
Bst4CI ACNGT 3 cut(s) 110, 343, 606
BstACI GRCGYC 1 cut(s) 189
BstAPI GCANNNNNTGC 1 cut(s) 495
BstBI TTCGAA 1 cut(s) 417
BstC8I GCNNGC 3 cut(s) 500, 513, 529
BstDEI CTNAG 1 cut(s) 51
BstMAI GTCTC 4 cut(s) 53, 116, 329, 591
BstMWI GCNNNNNNNGC 5 cut(s) 231, 495, 508, 528, 567
BstV2I GAAGAC 2 cut(s) 189, 406
BstXI CCANNNNNNTGG 1 cut(s) 253
BsuRI GGCC 1 cut(s) 377
Cac8I GCNNGC 3 cut(s) 500, 513, 529
CaiI CAGNNNCTG 1 cut(s) 62
CseI GACGC 3 cut(s) 24, 178, 573
Csp6I GTAC 1 cut(s) 106
CspCI CAANNNNNGTGG 2 cut(s) 136, 171
CviAII CATG 2 cut(s) 119, 247
CviQI GTAC 1 cut(s) 106
DdeI CTNAG 1 cut(s) 51
DriI GACNNNNNGTC 1 cut(s) 172
EaeI YGGCCR 1 cut(s) 375
Eam1105I GACNNNNNGTC 1 cut(s) 172
EciI GGCGGA 1 cut(s) 466
Eco130I CCWWGG 1 cut(s) 208
Eco31I GGTCTC 2 cut(s) 116, 329
Eco32I GATATC 1 cut(s) 467
Eco57I CTGAAG 1 cut(s) 84
EcoRV GATATC 1 cut(s) 467
EcoT14I CCWWGG 1 cut(s) 208
ErhI CCWWGG 1 cut(s) 208
Esp3I CGTCTC 1 cut(s) 591
FaeI CATG 2 cut(s) 122, 250
FaiI YATR 5 cut(s) 14, 120, 248, 411, 441
FatI CATG 2 cut(s) 118, 246
FauI CCCGC 2 cut(s) 507, 536
FblI GTMKAC 1 cut(s) 165
FspBI CTAG 3 cut(s) 231, 471, 543
HaeIII GGCC 1 cut(s) 377
HgaI GACGC 3 cut(s) 24, 178, 573
Hin1I GRCGYC 1 cut(s) 189
Hin1II CATG 2 cut(s) 122, 250
HincII GTYRAC 1 cut(s) 166
HindII GTYRAC 1 cut(s) 166
HinfI GANTC 3 cut(s) 267, 537, 554
HphI GGTGA 1 cut(s) 115
Hpy166II GTNNAC 1 cut(s) 166
Hpy188I TCNGA 1 cut(s) 591
Hpy8I GTNNAC 1 cut(s) 166
Hpy99I CGWCG 3 cut(s) 167, 182, 194
HpyAV CCTTC 2 cut(s) 23, 59
HpyCH4III ACNGT 3 cut(s) 110, 343, 606
HpyCH4IV ACGT 1 cut(s) 162
HpyCH4V TGCA 3 cut(s) 287, 293, 489
HpyF10VI GCNNNNNNNGC 5 cut(s) 231, 495, 508, 528, 567
HpyF3I CTNAG 1 cut(s) 51
HpySE526I ACGT 1 cut(s) 162
Hsp92I GRCGYC 1 cut(s) 189
Hsp92II CATG 2 cut(s) 122, 250
LmnI GCTCC 4 cut(s) 32, 210, 479, 566
LpnPI CCDG 8 cut(s) 38, 359, 378, 470, 529, 547, 564, 580
LweI GCATC 2 cut(s) 280, 343
MaeI CTAG 3 cut(s) 231, 471, 543
MaeII ACGT 1 cut(s) 162
MaeIII GTNAC 1 cut(s) 121
MboII GAAGA 7 cut(s) 32, 35, 38, 194, 335, 406, 445
MhlI GDGCHC 1 cut(s) 406
MlsI TGGCCA 1 cut(s) 377
MluCI AATT 2 cut(s) 384, 419
MluNI TGGCCA 1 cut(s) 377
MlyI GAGTC 1 cut(s) 276
MnlI CCTC 2 cut(s) 140, 565
Mox20I TGGCCA 1 cut(s) 377
MscI TGGCCA 1 cut(s) 377
MseI TTAA 2 cut(s) 273, 383
MslI CAYNNNNRTG 1 cut(s) 251
Msp20I TGGCCA 1 cut(s) 377
MwoI GCNNNNNNNGC 5 cut(s) 231, 495, 508, 528, 567
NlaIII CATG 2 cut(s) 122, 250
NmuCI GTSAC 1 cut(s) 121
NspV TTCGAA 1 cut(s) 417
PcsI WCGNNNNNNNCGW 1 cut(s) 186
PfeI GAWTC 2 cut(s) 537, 554
PflFI GACNNNGTC 1 cut(s) 188
PleI GAGTC 1 cut(s) 275
PpsI GAGTC 1 cut(s) 275
PstNI CAGNNNCTG 1 cut(s) 62
PsyI GACNNNGTC 1 cut(s) 188
RsaI GTAC 1 cut(s) 107
RsaNI GTAC 1 cut(s) 106
RseI CAYNNNNRTG 1 cut(s) 251
SalI GTCGAC 1 cut(s) 164
SaqAI TTAA 2 cut(s) 273, 383
SchI GAGTC 1 cut(s) 276
SduI GDGCHC 1 cut(s) 406
SfaNI GCATC 2 cut(s) 280, 343
SfuI TTCGAA 1 cut(s) 417
SmiMI CAYNNNNRTG 1 cut(s) 251
SmlI CTYRAG 1 cut(s) 200
SmoI CTYRAG 1 cut(s) 200
Sse9I AATT 2 cut(s) 384, 419
SsiI CCGC 4 cut(s) 477, 500, 525, 529
SspMI CTAG 3 cut(s) 231, 471, 543
StyI CCWWGG 1 cut(s) 208
TaaI ACNGT 3 cut(s) 110, 343, 606
TaiI ACGT 1 cut(s) 165
TaqI TCGA 3 cut(s) 165, 180, 417
TasI AATT 2 cut(s) 384, 419
TfiI GAWTC 2 cut(s) 537, 554
Tru1I TTAA 2 cut(s) 273, 383
Tru9I TTAA 2 cut(s) 273, 383
TseFI GTSAC 1 cut(s) 121
Tsp45I GTSAC 1 cut(s) 121
TspDTI ATGAA 3 cut(s) 263, 336, 456
TspGWI ACGGA 1 cut(s) 166
Tth111I GACNNNGTC 1 cut(s) 188
XcmI CCANNNNNNNNNTGG 2 cut(s) 215, 250
XmiI GTMKAC 1 cut(s) 165
XspI CTAG 3 cut(s) 231, 471, 543
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.