Rh7DG118800

NifU-like protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0017763
Physical Location & Seq
Reverse (-)
9980851 .. 10004276
23426 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG118800.1

Sequence Viewer

Length: 627 bp
ATGCATGTGGTGAGTGAGAGAATCCCAAGCTTATTGGAGGCGACTGCACCATTCTTCGAACTGATTTGCACGCTGACTTACCACTTTCCTGTGGTGACCATCAATGATGGTGGAAACGTCGACATTGTGTCCGTCGAAGACGGCGTCGTTTCTCTCAAGCTCCAAGGGGCTTGTGGAAGTTGTCCTAGCTCAACAACCACCATGAAAATGGGGATTGAACGAGTGCTTAAAGAAAAGTTTGCAGATGCACTCAAGGATATTCAACAAGTCTTTGATGAAGAAAACAGAGAGACCACAGTTGAGGTAACGAGTGTTGGAAGCAATACAGGATGGGGATTGTTGATGGCAAGTATCTCTGAGGATACCGGTGAAGAAGCTCCTTTACAGGTTAGAAAAACTGATAGAATATCCCTAAAATGGTCTGACAGATTGAGGGAAACTCTTGACTTGACACACCCACGAGTTGCCTGTTTGGAAAGTAAAATAAGGGAAAAGGTCAGGGGAAAGTTGAATGAACGCTGGAAGCATTGGAAGTCTGAGTTGAGCAAGAAATGGTATAAACCATTCAAGAATAGTCCCCTTAGGTTTCGCAAACCTGATGATGATCGAGTTGATGCGGACCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005198 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NifU

Protein Analysis

208

Amino Acids

23.62

Weight (kDa)

6.14

Isoelectric Point (pI)

39.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NifU PF01106 35 - 81 4.7e-18 NifU-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017763)

Species Orthologous Gene IDs
pyrus_communis pycom16g20660
rosa_chinensis RchiOBHm_Chr2g0124791 RchiOBHm_Chr2g0133041
rosa_laevigata RLG00000014296
rosa_roxburghii Rroxscaffold_1G00004640 Rroxscaffold_6G00396430
rosa_samantha Rh5BG519700 Rh5CG543700 Rh7BG392600 Rh7DG118800 Rh7DG442200
rosa_wichuraiana Rw7G024550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 120
AciI CCGC 1 cut(s) 617
AcyI GRCGYC 1 cut(s) 144
AfiI CCNNNNNNNGG 1 cut(s) 417
AgeI ACCGGT 1 cut(s) 365
AgsI TTSAA 4 cut(s) 218, 263, 511, 568
AhdI GACNNNNNGTC 1 cut(s) 127
AluBI AGCT 4 cut(s) 30, 160, 189, 377
AluI AGCT 4 cut(s) 30, 160, 189, 377
Alw26I GTCTC 1 cut(s) 284
ArsI GACNNNNNNTTYG 2 cut(s) 129, 161
AsiGI ACCGGT 1 cut(s) 365
AspS9I GGNCC 1 cut(s) 619
AsuHPI GGTGA 3 cut(s) 22, 106, 380
AsuII TTCGAA 1 cut(s) 57
AvaII GGWCC 1 cut(s) 619
AxyI CCTNAGG 1 cut(s) 581
BarI GAAGNNNNNNTAC 2 cut(s) 366, 398
BauI CACGAG 1 cut(s) 459
BbsI GAAGAC 1 cut(s) 144
BccI CCATC 4 cut(s) 101, 107, 324, 337
BceAI ACGGC 1 cut(s) 157
BciVI GTATCC 1 cut(s) 355
BcoDI GTCTC 1 cut(s) 284
BfaI CTAG 1 cut(s) 186
BfuI GTATCC 1 cut(s) 355
Bme18I GGWCC 1 cut(s) 619
BmeRI GACNNNNNGTC 1 cut(s) 127
BmgT120I GGNCC 1 cut(s) 619
BmsI GCATC 2 cut(s) 235, 604
BpiI GAAGAC 1 cut(s) 144
BplI GAGNNNNNCTC 2 cut(s) 424, 456
Bpu14I TTCGAA 1 cut(s) 57
BpuEI CTTGAG 2 cut(s) 140, 236
BsaBI GATNNNNATC 1 cut(s) 603
BsaHI GRCGYC 1 cut(s) 144
BsaI GGTCTC 1 cut(s) 284
BsaJI CCNNGG 1 cut(s) 163
BsaWI WCCGGW 1 cut(s) 365
Bsc4I CCNNNNNNNGG 1 cut(s) 417
Bse118I RCCGGY 1 cut(s) 365
Bse21I CCTNAGG 1 cut(s) 581
Bse8I GATNNNNATC 1 cut(s) 603
BseDI CCNNGG 1 cut(s) 163
BseGI GGATG 1 cut(s) 335
BseJI GATNNNNATC 1 cut(s) 603
BseLI CCNNNNNNNGG 1 cut(s) 417
BseMII CTCAG 2 cut(s) 348, 528
BsgI GTGCAG 1 cut(s) 30
BshTI ACCGGT 1 cut(s) 365
BsiSI CCGG 1 cut(s) 366
BslFI GGGAC 1 cut(s) 561
BslI CCNNNNNNNGG 1 cut(s) 417
BsmAI GTCTC 1 cut(s) 284
BsmFI GGGAC 1 cut(s) 561
Bso31I GGTCTC 1 cut(s) 284
Bsp119I TTCGAA 1 cut(s) 57
Bsp143I GATC 1 cut(s) 604
BspACI CCGC 1 cut(s) 617
BspCNI CTCAG 2 cut(s) 349, 529
BspT104I TTCGAA 1 cut(s) 57
BspTNI GGTCTC 1 cut(s) 284
BsrFI RCCGGY 1 cut(s) 365
BssAI RCCGGY 1 cut(s) 365
BssECI CCNNGG 1 cut(s) 163
BssMI GATC 1 cut(s) 604
BssNI GRCGYC 1 cut(s) 144
BssSI CACGAG 1 cut(s) 459
BssT1I CCWWGG 1 cut(s) 163
Bst2BI CACGAG 1 cut(s) 459
Bst4CI ACNGT 1 cut(s) 298
BstACI GRCGYC 1 cut(s) 144
BstBI TTCGAA 1 cut(s) 57
BstC8I GCNNGC 1 cut(s) 71
BstDEI CTNAG 3 cut(s) 357, 537, 581
BstEII GGTNACC 1 cut(s) 94
BstF5I GGATG 1 cut(s) 335
BstKTI GATC 1 cut(s) 607
BstMAI GTCTC 1 cut(s) 284
BstMBI GATC 1 cut(s) 604
BstNSI RCATGY 1 cut(s) 8
BstPI GGTNACC 1 cut(s) 94
BstV2I GAAGAC 1 cut(s) 144
BstXI CCANNNNNNTGG 1 cut(s) 208
Bsu36I CCTNAGG 1 cut(s) 581
BsuI GTATCC 1 cut(s) 355
BtsCI GGATG 1 cut(s) 335
Cac8I GCNNGC 1 cut(s) 71
Cfr10I RCCGGY 1 cut(s) 365
Cfr13I GGNCC 1 cut(s) 619
CseI GACGC 1 cut(s) 133
CspAI ACCGGT 1 cut(s) 365
CspCI CAANNNNNGTGG 2 cut(s) 91, 126
CviAII CATG 2 cut(s) 5, 202
CviJI RGCY 5 cut(s) 30, 160, 170, 189, 377
CviKI_1 RGCY 5 cut(s) 30, 160, 170, 189, 377
DdeI CTNAG 3 cut(s) 357, 537, 581
DpnI GATC 1 cut(s) 606
DpnII GATC 1 cut(s) 604
DriI GACNNNNNGTC 1 cut(s) 127
Eam1105I GACNNNNNGTC 1 cut(s) 127
Eco130I CCWWGG 1 cut(s) 163
Eco31I GGTCTC 1 cut(s) 284
Eco47I GGWCC 1 cut(s) 619
Eco81I CCTNAGG 1 cut(s) 581
Eco91I GGTNACC 1 cut(s) 94
EcoO65I GGTNACC 1 cut(s) 94
EcoT14I CCWWGG 1 cut(s) 163
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 163
FaeI CATG 2 cut(s) 8, 205
FaiI YATR 3 cut(s) 6, 203, 558
FaqI GGGAC 1 cut(s) 561
FatI CATG 2 cut(s) 4, 201
FblI GTMKAC 1 cut(s) 120
FokI GGATG 1 cut(s) 342
FspBI CTAG 1 cut(s) 186
HapII CCGG 1 cut(s) 366
HgaI GACGC 1 cut(s) 133
Hin1I GRCGYC 1 cut(s) 144
Hin1II CATG 2 cut(s) 8, 205
HincII GTYRAC 1 cut(s) 121
HindII GTYRAC 1 cut(s) 121
HindIII AAGCTT 1 cut(s) 28
HinfI GANTC 1 cut(s) 21
HpaII CCGG 1 cut(s) 366
HphI GGTGA 3 cut(s) 22, 106, 380
Hpy166II GTNNAC 1 cut(s) 121
Hpy188I TCNGA 3 cut(s) 358, 424, 538
Hpy188III TCNNGA 2 cut(s) 443, 568
Hpy8I GTNNAC 1 cut(s) 121
Hpy99I CGWCG 3 cut(s) 122, 137, 149
HpyCH4III ACNGT 1 cut(s) 298
HpyCH4IV ACGT 1 cut(s) 117
HpyCH4V TGCA 5 cut(s) 4, 47, 69, 242, 248
HpyF3I CTNAG 3 cut(s) 357, 537, 581
HpySE526I ACGT 1 cut(s) 117
Hsp92I GRCGYC 1 cut(s) 144
Hsp92II CATG 2 cut(s) 8, 205
Kzo9I GATC 1 cut(s) 604
LmnI GCTCC 2 cut(s) 165, 382
LpnPI CCDG 8 cut(s) 102, 312, 371, 379, 481, 484, 505, 609
LweI GCATC 2 cut(s) 235, 604
MaeI CTAG 1 cut(s) 186
MaeII ACGT 1 cut(s) 117
MaeIII GTNAC 2 cut(s) 94, 304
MalI GATC 1 cut(s) 606
MboI GATC 1 cut(s) 604
MboII GAAGA 4 cut(s) 46, 149, 290, 383
MmeI TCCRAC 1 cut(s) 295
MnlI CCTC 4 cut(s) 31, 295, 352, 426
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 1 cut(s) 228
MslI CAYNNNNRTG 1 cut(s) 206
MspI CCGG 1 cut(s) 366
NdeII GATC 1 cut(s) 604
NlaIII CATG 2 cut(s) 8, 205
NmuCI GTSAC 1 cut(s) 94
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 8
NspV TTCGAA 1 cut(s) 57
PcsI WCGNNNNNNNCGW 1 cut(s) 141
PfeI GAWTC 1 cut(s) 21
PflFI GACNNNGTC 1 cut(s) 143
PinAI ACCGGT 1 cut(s) 365
PspEI GGTNACC 1 cut(s) 94
PspPI GGNCC 1 cut(s) 619
PsyI GACNNNGTC 1 cut(s) 143
RseI CAYNNNNRTG 1 cut(s) 206
SalI GTCGAC 1 cut(s) 119
SaqAI TTAA 1 cut(s) 228
Sau3AI GATC 1 cut(s) 604
Sau96I GGNCC 1 cut(s) 619
SfaNI GCATC 2 cut(s) 235, 604
SfuI TTCGAA 1 cut(s) 57
SinI GGWCC 1 cut(s) 619
SmiMI CAYNNNNRTG 1 cut(s) 206
SmlI CTYRAG 2 cut(s) 155, 251
SmoI CTYRAG 2 cut(s) 155, 251
SsiI CCGC 1 cut(s) 617
SspMI CTAG 1 cut(s) 186
StyI CCWWGG 1 cut(s) 163
TaaI ACNGT 1 cut(s) 298
TaiI ACGT 1 cut(s) 120
TaqI TCGA 4 cut(s) 57, 120, 135, 607
TfiI GAWTC 1 cut(s) 21
Tru1I TTAA 1 cut(s) 228
Tru9I TTAA 1 cut(s) 228
TseFI GTSAC 1 cut(s) 94
Tsp45I GTSAC 1 cut(s) 94
TspDTI ATGAA 3 cut(s) 218, 291, 528
TspGWI ACGGA 1 cut(s) 121
Tth111I GACNNNGTC 1 cut(s) 143
VpaK11BI GGWCC 1 cut(s) 619
XceI RCATGY 1 cut(s) 8
XcmI CCANNNNNNNNNTGG 2 cut(s) 170, 205
XmiI GTMKAC 1 cut(s) 120
XspI CTAG 1 cut(s) 186
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.