Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5D

Orthogroup ID

OG0008574

Physical Location & Seq

Genomic Coordinates Forward (+)

53138970 .. 53165609

Gene Structure 26640 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5DG357500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1698 bp

ATGGTTGTGGAGACTTTGTTCCCGGGTCACTCCGACCTGTACAGTACAATCTCGACCCGGGTAACTTCGGCTGCATTTGGAATTGACGCGATTATCTGGGCAAGCGGGTCGAGGAATGGCTATCGCAGTTTAGGCCGATACAAGTCAATATCCACCGGAGACCCTCACACCATGGAAGAGTCAAATGAAGTTCATTCAGAGGAGAATAAAACTTCCTTTGATAATAAGAAAAGAGTACTCAAGACACCAGCCCAGCTTACGGCTTTGGAGAAATTTTATAATGAGCATAAGTATCCTACGGAGGAAATGAAATCACAACTCGCTGAGGAGTTGGGATTGTCAGAAAAGCAGATATCTGGATGGTTTTGCCACAGAAGGTTAAAAGACAAAAGATTGTCTAGAGATGAAACATATGCTAATGGACGGCAAGATCGTTCAAGTGGTGTTATTCAGGATCGCGCTAGTGGACTTGGGCAAGACTCATGTGGTAGCACTAAACATGGAGACTACCGGTACGTTGATCCTAGGGAGGTTGAAAGTCGGAGGCTTTCTGGGTATGATTTCCCACCCGCAGATCTCACTCGTGAGCATCGGATTCATTATACAGAAAGAGTTAGCATGGATAATACATCTTCAGAAAGTAGCTCATCTTTACAAGATAGGTTTCTTACTCAAACTGAAGATCCATATGATATGGAAACATCTAGATACATAACGGATAATGGGGTCTTTTCACCCTTAAATACGAAAGGCGCAGAAAACATGGGATATAAGCCCTCAGGGTATTTGAAAGTCAAAGGCGAAATAGAAAATGCTGCTATTACAGCTGTTAAGAGGCAACTTGGGAGGCATTTTCGGGAGGATGGTCCTCCATTAGGTGTAGAATTTGATCCACTTCCTCCAGGTGCATTTGAATCCCCGATTATGGATCCAGTGCAAGAACCATCAATTGGTGGGGATGCTGCTGGTCTTGATTCCCCTGACACATCTGGAGTAAAAAGGCAACCAAGTCCAAGCACTAGATATGAAGTATATAATTCCAAGTTGAGTTCCCAGGGTTTGTATATGCACCAGGAAAATCGTGGTATAATGCATGGGGTTGATATGGTGGAGAGGAAATCTCGCAAGCAATTGAGGCAAAAGTCCACTTATCTTAAAGATATCAATTCTTCTGCTGGCAAAAACTCTTCCTTGGATATGTATGATGCATTTCCTGGAGGAATGTCTGCTTATAATGGCAATAGGAAGAACAGAATGAGCTCTAAGCATGGCCACCGTGGTAGGAAAATCACTAGTGAGACAACAGAGCCATGGTCAAATGAGTCCGATAAAGTTTGTAGTCCCAACATTGGCCAAAGAAGCGATTACTTTAAGTCAAAGGCTTTAAATTCTGTAGTTAAGCATTCCGAAACTCATGACACAGAGAGAGGACTATTTACAATGATCCCACGGGAGGAGAAAGTTGATGGAGAATGTAAGGCAGTCAAAGATTATCACAATCCACTTAGAGTAACGATGACAGATGAAGTGGCGGTTGGAAAACAAGGCAGAGTTAATTTCCCTCACCAAGACTATGCAACAAGTGTGCCGTTTTCTCAAATTCCAGAGCGTAAAAATCAGATTAAAGGAACTGCCATGGAGATGCCATCTAGCTTCAGTGAGGATGAGACTGCAGAAACAAACTCTTCGGTTGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Homeobox

Protein Analysis

565

Amino Acids

63.41

Weight (kDa)

6.41

Isoelectric Point (pI)

53.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Homeodomain	PF00046	76 - 131	1.3e-17	Homeodomain
Homeobox_KN	PF05920	96 - 126	1.9e-07	Homeobox KN domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008574)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G03250
fragaria_vesca	FvH4_3g27990 FvH4_3g27990 FvH4_3g27990 FvH4_3g27990
malus_domestica	MD03G1167600.v1.1 MD11G1186500.v1.1
prunus_persica	Prupe.4G267700_v2.0.a1 Prupe.4G267700_v2.0.a1
pyrus_communis	pycom03g12280 pycom11g16120
rosa_chinensis	RchiOBHm_Chr5g0050681
rosa_laevigata	RLG00000034723
rosa_multiflora	Rmu_sc0007911.1_g000002 Rmu_ssc0000350.1_g000043
rosa_roxburghii	Rroxscaffold_1G00030120
rosa_rugosa	Rorug05G0263100 Rorug05G0263200
rosa_samantha	Rh5AG335000 Rh5BG344300 Rh5CG372400 Rh5DG357500
rosa_wichuraiana	Rw5G031610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	279, 1233
AccB7I	CCANNNNNTGG	1 cut(s)	950
AccII	CGCG	2 cut(s)	89, 459
AciI	CCGC	3 cut(s)	105, 570, 1532
AclWI	GGATC	7 cut(s)	462, 515, 677, 884, 923, 936, 1438
AcoI	YGGCCR	2 cut(s)	1270, 1351
AcsI	RAATTY	4 cut(s)	272, 884, 1387, 1599
AcuI	CTGAAG	3 cut(s)	618, 699, 1639
AfaI	GTAC	4 cut(s)	41, 46, 237, 515
AfiI	CCNNNNNNNGG	6 cut(s)	259, 875, 925, 950, 1349, 1453
AgeI	ACCGGT	1 cut(s)	510
AgsI	TTSAA	4 cut(s)	438, 536, 790, 914
AhlI	ACTAGT	1 cut(s)	1292
AjnI	CCWGG	4 cut(s)	901, 1053, 1071, 1213
AjuI	GAANNNNNNNTTGG	4 cut(s)	933, 965, 1518, 1550
AloI	GAACNNNNNNTCC	1 cut(s)	34
AluBI	AGCT	5 cut(s)	256, 645, 827, 1260, 1653
AluI	AGCT	5 cut(s)	256, 645, 827, 1260, 1653
Alw21I	GWGCWC	1 cut(s)	1262
Alw26I	GTCTC	5 cut(s)	5, 153, 498, 1292, 1661
AlwI	GGATC	7 cut(s)	462, 515, 677, 884, 923, 936, 1438
Ama87I	CYCGRG	2 cut(s)	22, 57
AoxI	GGCC	3 cut(s)	133, 1270, 1351
ApeKI	GCWGC	3 cut(s)	71, 815, 962
ApoI	RAATTY	4 cut(s)	272, 884, 1387, 1599
AsiGI	ACCGGT	1 cut(s)	510
AspA2I	CCTAGG	1 cut(s)	524
AspLEI	GCGC	2 cut(s)	461, 755
AspS9I	GGNCC	1 cut(s)	866
AsuC2I	CCSGG	4 cut(s)	23, 24, 58, 59
AsuHPI	GGTGA	2 cut(s)	726, 1556
AvaI	CYCGRG	2 cut(s)	22, 57
AvaII	GGWCC	1 cut(s)	866
AvrII	CCTAGG	1 cut(s)	524
AxyI	CCTNAGG	1 cut(s)	778
BalI	TGGCCA	2 cut(s)	1272, 1353
BamHI	GGATCC	1 cut(s)	928
BanII	GRGCYC	1 cut(s)	1262
BauI	CACGAG	1 cut(s)	582
Bbv12I	GWGCWC	1 cut(s)	1262
BbvCI	CCTCAGC	1 cut(s)	324
BbvI	GCAGC	3 cut(s)	58, 802, 949
BccI	CCATC	5 cut(s)	354, 857, 952, 1460, 1654
BceAI	ACGGC	3 cut(s)	276, 440, 1573
BcgI	CGANNNNNNTGC	2 cut(s)	90, 124
BciT130I	CCWGG	4 cut(s)	903, 1055, 1073, 1215
BciVI	GTATCC	1 cut(s)	303
BcnI	CCSGG	4 cut(s)	23, 24, 58, 59
BcoDI	GTCTC	5 cut(s)	5, 153, 498, 1292, 1661
BcuI	ACTAGT	1 cut(s)	1292
BfaI	CTAG	7 cut(s)	399, 462, 525, 705, 1020, 1293, 1650
BfmI	CTRYAG	2 cut(s)	1392, 1671
BfuI	GTATCC	1 cut(s)	303
BglII	AGATCT	1 cut(s)	574
BisI	GCNGC	3 cut(s)	72, 816, 963
BlnI	CCTAGG	1 cut(s)	524
BlsI	GCNGC	3 cut(s)	73, 817, 964
BmcAI	AGTACT	1 cut(s)	237
Bme1390I	CCNGG	8 cut(s)	23, 24, 58, 59, 903, 1055, 1073, 1215
Bme18I	GGWCC	1 cut(s)	866
BmeT110I	CYCGRG	2 cut(s)	22, 57
BmgT120I	GGNCC	1 cut(s)	866
BmiI	GGNNCC	1 cut(s)	930
BmrFI	CCNGG	8 cut(s)	23, 24, 58, 59, 903, 1055, 1073, 1215
BmsI	GCATC	4 cut(s)	598, 949, 1195, 1632
BplI	GAGNNNNNCTC	2 cut(s)	1105, 1137
BpmI	CTGGAG	3 cut(s)	885, 1011, 1236
Bpu10I	CCTNAGC	1 cut(s)	324
BpuEI	CTTGAG	1 cut(s)	224
BpuMI	CCSGG	4 cut(s)	23, 24, 58, 59
BsaI	GGTCTC	1 cut(s)	153
BsaWI	WCCGGW	2 cut(s)	155, 510
BsaXI	ACNNNNNCTCC	1 cut(s)	32
Bsc4I	CCNNNNNNNGG	6 cut(s)	259, 875, 925, 950, 1349, 1453
Bse118I	RCCGGY	1 cut(s)	510
Bse1I	ACTGG	1 cut(s)	932
Bse21I	CCTNAGG	1 cut(s)	778
BseBI	CCWGG	4 cut(s)	903, 1055, 1073, 1215
BseGI	GGATG	4 cut(s)	365, 868, 964, 1669
BseLI	CCNNNNNNNGG	6 cut(s)	259, 875, 925, 950, 1349, 1453
BseMII	CTCAG	2 cut(s)	315, 792
BseNI	ACTGG	1 cut(s)	932
BseRI	GAGGAG	3 cut(s)	215, 341, 1469
BseXI	GCAGC	3 cut(s)	58, 802, 949
BseYI	CCCAGC	1 cut(s)	252
Bsh1236I	CGCG	2 cut(s)	89, 459
BshFI	GGCC	3 cut(s)	135, 1272, 1353
BshTI	ACCGGT	1 cut(s)	510
BsiHKAI	GWGCWC	1 cut(s)	1262
BsiHKCI	CYCGRG	2 cut(s)	22, 57
BsiSI	CCGG	4 cut(s)	23, 58, 156, 511
BslFI	GGGAC	1 cut(s)	1326
BslI	CCNNNNNNNGG	6 cut(s)	259, 875, 925, 950, 1349, 1453
BsmAI	GTCTC	5 cut(s)	5, 153, 498, 1292, 1661
BsmFI	GGGAC	1 cut(s)	1326
BsmI	GAATGC	1 cut(s)	1402
BsnI	GGCC	3 cut(s)	135, 1272, 1353
Bso31I	GGTCTC	1 cut(s)	153
BsoBI	CYCGRG	2 cut(s)	22, 57
Bsp1286I	GDGCHC	1 cut(s)	1262
Bsp1407I	TGTACA	1 cut(s)	39
Bsp143I	GATC	8 cut(s)	430, 454, 520, 574, 682, 889, 928, 1443
Bsp19I	CCATGG	3 cut(s)	171, 1310, 1635
BspACI	CCGC	3 cut(s)	105, 570, 1532
BspANI	GGCC	3 cut(s)	135, 1272, 1353
BspCNI	CTCAG	2 cut(s)	316, 791
BspFNI	CGCG	2 cut(s)	89, 459
BspHI	TCATGA	1 cut(s)	1414
BspLI	GGNNCC	1 cut(s)	930
BspMAI	CTGCAG	1 cut(s)	1675
BspPI	GGATC	7 cut(s)	462, 515, 677, 884, 923, 936, 1438
BspTNI	GGTCTC	1 cut(s)	153
BsrFI	RCCGGY	1 cut(s)	510
BsrGI	TGTACA	1 cut(s)	39
BsrI	ACTGG	1 cut(s)	932
BssAI	RCCGGY	1 cut(s)	510
BssMI	GATC	8 cut(s)	430, 454, 520, 574, 682, 889, 928, 1443
BssSI	CACGAG	1 cut(s)	582
BssT1I	CCWWGG	5 cut(s)	171, 524, 1191, 1310, 1635
Bst2BI	CACGAG	1 cut(s)	582
Bst2UI	CCWGG	4 cut(s)	903, 1055, 1073, 1215
Bst4CI	ACNGT	2 cut(s)	44, 1277
Bst6I	CTCTTC	3 cut(s)	171, 1192, 1690
BstAUI	TGTACA	1 cut(s)	39
BstC8I	GCNNGC	3 cut(s)	103, 1127, 1177
BstDEI	CTNAG	4 cut(s)	324, 778, 1263, 1505
BstDSI	CCRYGG	5 cut(s)	171, 1276, 1310, 1448, 1635
BstENI	CCTNNNNNAGG	1 cut(s)	873
BstF5I	GGATG	4 cut(s)	365, 868, 964, 1669
BstFNI	CGCG	2 cut(s)	89, 459
BstHHI	GCGC	2 cut(s)	461, 755
BstKTI	GATC	8 cut(s)	433, 457, 523, 577, 685, 892, 931, 1446
BstMAI	GTCTC	5 cut(s)	5, 153, 498, 1292, 1661
BstMBI	GATC	8 cut(s)	430, 454, 520, 574, 682, 889, 928, 1443
BstMWI	GCNNNNNNNGC	4 cut(s)	132, 824, 1135, 1359
BstNI	CCWGG	4 cut(s)	903, 1055, 1073, 1215
BstSCI	CCNGG	8 cut(s)	21, 22, 56, 57, 901, 1053, 1071, 1213
BstSFI	CTRYAG	2 cut(s)	1392, 1671
BstUI	CGCG	2 cut(s)	89, 459
BstV1I	GCAGC	3 cut(s)	58, 802, 949
BstX2I	RGATCY	3 cut(s)	574, 682, 928
BstYI	RGATCY	3 cut(s)	574, 682, 928
Bsu36I	CCTNAGG	1 cut(s)	778
BsuI	GTATCC	1 cut(s)	303
BsuRI	GGCC	3 cut(s)	135, 1272, 1353
BtgI	CCRYGG	5 cut(s)	171, 1276, 1310, 1448, 1635
BtsCI	GGATG	4 cut(s)	365, 868, 964, 1669
BtsIMutI	CAGTG	2 cut(s)	939, 1663
Cac8I	GCNNGC	3 cut(s)	103, 1127, 1177
CciI	TCATGA	1 cut(s)	1414
CfoI	GCGC	2 cut(s)	461, 755
Cfr10I	RCCGGY	1 cut(s)	510
Cfr13I	GGNCC	1 cut(s)	866
Cfr9I	CCCGGG	2 cut(s)	22, 57
CseI	GACGC	1 cut(s)	95
Csp6I	GTAC	4 cut(s)	40, 45, 236, 514
CspAI	ACCGGT	1 cut(s)	510
CviQI	GTAC	4 cut(s)	40, 45, 236, 514
DdeI	CTNAG	4 cut(s)	324, 778, 1263, 1505
DpnI	GATC	8 cut(s)	432, 456, 522, 576, 684, 891, 930, 1445
DpnII	GATC	8 cut(s)	430, 454, 520, 574, 682, 889, 928, 1443
DraI	TTTAAA	1 cut(s)	1386
EaeI	YGGCCR	2 cut(s)	1270, 1351
Eam1104I	CTCTTC	3 cut(s)	171, 1192, 1690
EarI	CTCTTC	3 cut(s)	171, 1192, 1690
Ecl136II	GAGCTC	1 cut(s)	1260
Eco130I	CCWWGG	5 cut(s)	171, 524, 1191, 1310, 1635
Eco24I	GRGCYC	1 cut(s)	1262
Eco31I	GGTCTC	1 cut(s)	153
Eco32I	GATATC	2 cut(s)	354, 1162
Eco47I	GGWCC	1 cut(s)	866
Eco53kI	GAGCTC	1 cut(s)	1260
Eco57I	CTGAAG	3 cut(s)	618, 699, 1639
Eco81I	CCTNAGG	1 cut(s)	778
Eco88I	CYCGRG	2 cut(s)	22, 57
EcoICRI	GAGCTC	1 cut(s)	1260
EcoNI	CCTNNNNNAGG	1 cut(s)	873
EcoRII	CCWGG	4 cut(s)	901, 1053, 1071, 1213
EcoRV	GATATC	2 cut(s)	354, 1162
EcoT14I	CCWWGG	5 cut(s)	171, 524, 1191, 1310, 1635
EcoT22I	ATGCAT	2 cut(s)	1095, 1210
EcoT38I	GRGCYC	1 cut(s)	1262
ErhI	CCWWGG	5 cut(s)	171, 524, 1191, 1310, 1635
FaqI	GGGAC	1 cut(s)	1326
FauI	CCCGC	2 cut(s)	98, 577
FauNDI	CATATG	2 cut(s)	412, 688
Fnu4HI	GCNGC	3 cut(s)	72, 816, 963
FokI	GGATG	4 cut(s)	372, 875, 971, 1676
FriOI	GRGCYC	1 cut(s)	1262
Fsp4HI	GCNGC	3 cut(s)	72, 816, 963
FspBI	CTAG	7 cut(s)	399, 462, 525, 705, 1020, 1293, 1650
GlaI	GCGC	2 cut(s)	460, 754
GluI	GCNGC	3 cut(s)	72, 816, 963
GsaI	CCCAGC	1 cut(s)	256
GsuI	CTGGAG	3 cut(s)	885, 1011, 1236
HaeIII	GGCC	3 cut(s)	135, 1272, 1353
HapII	CCGG	4 cut(s)	23, 58, 156, 511
HgaI	GACGC	1 cut(s)	95
HhaI	GCGC	2 cut(s)	461, 755
Hin6I	GCGC	2 cut(s)	459, 753
HinP1I	GCGC	2 cut(s)	459, 753
HinfI	GANTC	6 cut(s)	179, 479, 595, 914, 974, 1322
HpaII	CCGG	4 cut(s)	23, 58, 156, 511
HphI	GGTGA	2 cut(s)	726, 1556
Hpy166II	GTNNAC	2 cut(s)	467, 1146
Hpy188I	TCNGA	9 cut(s)	34, 199, 343, 543, 594, 637, 1327, 1408, 1620
Hpy8I	GTNNAC	2 cut(s)	467, 1146
HpyAV	CCTTC	1 cut(s)	369
HpyCH4III	ACNGT	2 cut(s)	44, 1277
HpyCH4IV	ACGT	1 cut(s)	516
HpyCH4V	TGCA	8 cut(s)	74, 908, 937, 1069, 1093, 1208, 1577, 1673
HpyF10VI	GCNNNNNNNGC	4 cut(s)	132, 824, 1135, 1359
HpyF3I	CTNAG	4 cut(s)	324, 778, 1263, 1505
HpySE526I	ACGT	1 cut(s)	516
HspAI	GCGC	2 cut(s)	459, 753
Kzo9I	GATC	8 cut(s)	430, 454, 520, 574, 682, 889, 928, 1443
Lsp1109I	GCAGC	3 cut(s)	58, 802, 949
LweI	GCATC	4 cut(s)	598, 949, 1195, 1632
MaeI	CTAG	7 cut(s)	399, 462, 525, 705, 1020, 1293, 1650
MaeII	ACGT	1 cut(s)	516
MaeIII	GTNAC	3 cut(s)	26, 61, 1510
MalI	GATC	8 cut(s)	432, 456, 522, 576, 684, 891, 930, 1445
MboI	GATC	8 cut(s)	430, 454, 520, 574, 682, 889, 928, 1443
MboII	GAAGA	7 cut(s)	188, 624, 692, 1161, 1179, 1258, 1677
MfeI	CAATTG	2 cut(s)	948, 1130
MflI	RGATCY	3 cut(s)	574, 682, 928
MhlI	GDGCHC	1 cut(s)	1262
MlsI	TGGCCA	2 cut(s)	1272, 1353
MluNI	TGGCCA	2 cut(s)	1272, 1353
MlyI	GAGTC	3 cut(s)	188, 473, 1331
MmeI	TCCRAC	3 cut(s)	57, 521, 1516
Mox20I	TGGCCA	2 cut(s)	1272, 1353
Mph1103I	ATGCAT	2 cut(s)	1095, 1210
MscI	TGGCCA	2 cut(s)	1272, 1353
MseI	TTAA	9 cut(s)	380, 740, 831, 1155, 1371, 1385, 1398, 1554, 1623
MslI	CAYNNNNRTG	1 cut(s)	1640
Msp20I	TGGCCA	2 cut(s)	1272, 1353
MspA1I	CMGCKG	1 cut(s)	827
MspI	CCGG	4 cut(s)	23, 58, 156, 511
MspR9I	CCNGG	8 cut(s)	23, 24, 58, 59, 903, 1055, 1073, 1215
MunI	CAATTG	2 cut(s)	948, 1130
Mva1269I	GAATGC	1 cut(s)	1402
MvaI	CCWGG	4 cut(s)	903, 1055, 1073, 1215
MvnI	CGCG	2 cut(s)	89, 459
MwoI	GCNNNNNNNGC	4 cut(s)	132, 824, 1135, 1359
NciI	CCSGG	4 cut(s)	23, 24, 58, 59
NcoI	CCATGG	3 cut(s)	171, 1310, 1635
NdeI	CATATG	2 cut(s)	412, 688
NdeII	GATC	8 cut(s)	430, 454, 520, 574, 682, 889, 928, 1443
NlaIV	GGNNCC	1 cut(s)	930
NmuCI	GTSAC	1 cut(s)	26
NsiI	ATGCAT	2 cut(s)	1095, 1210
PagI	TCATGA	1 cut(s)	1414
PasI	CCCWGGG	1 cut(s)	1054
PcsI	WCGNNNNNNNCGW	1 cut(s)	430
PctI	GAATGC	1 cut(s)	1402
PfeI	GAWTC	3 cut(s)	595, 914, 974
PflMI	CCANNNNNTGG	1 cut(s)	950
PfoI	TCCNGGA	1 cut(s)	1213
PinAI	ACCGGT	1 cut(s)	510
PkrI	GCNGC	3 cut(s)	73, 817, 964
PleI	GAGTC	3 cut(s)	187, 473, 1330
PpsI	GAGTC	3 cut(s)	187, 473, 1330
PsiI	TTATAA	2 cut(s)	279, 1233
Psp124BI	GAGCTC	1 cut(s)	1262
Psp6I	CCWGG	4 cut(s)	901, 1053, 1071, 1213
PspFI	CCCAGC	1 cut(s)	252
PspGI	CCWGG	4 cut(s)	901, 1053, 1071, 1213
PspN4I	GGNNCC	1 cut(s)	930
PspPI	GGNCC	1 cut(s)	866
PstI	CTGCAG	1 cut(s)	1675
PsuI	RGATCY	3 cut(s)	574, 682, 928
PvuII	CAGCTG	1 cut(s)	827
RsaI	GTAC	4 cut(s)	41, 46, 237, 515
RsaNI	GTAC	4 cut(s)	40, 45, 236, 514
RseI	CAYNNNNRTG	1 cut(s)	1640
SacI	GAGCTC	1 cut(s)	1262
SaqAI	TTAA	9 cut(s)	380, 740, 831, 1155, 1371, 1385, 1398, 1554, 1623
SatI	GCNGC	3 cut(s)	72, 816, 963
Sau3AI	GATC	8 cut(s)	430, 454, 520, 574, 682, 889, 928, 1443
Sau96I	GGNCC	1 cut(s)	866
ScaI	AGTACT	1 cut(s)	237
SchI	GAGTC	3 cut(s)	188, 473, 1331
ScrFI	CCNGG	8 cut(s)	23, 24, 58, 59, 903, 1055, 1073, 1215
SduI	GDGCHC	1 cut(s)	1262
SfaNI	GCATC	4 cut(s)	598, 949, 1195, 1632
SfcI	CTRYAG	2 cut(s)	1392, 1671
SinI	GGWCC	1 cut(s)	866
SmaI	CCCGGG	2 cut(s)	24, 59
SmiMI	CAYNNNNRTG	1 cut(s)	1640
SmlI	CTYRAG	1 cut(s)	239
SmoI	CTYRAG	1 cut(s)	239
SpeI	ACTAGT	1 cut(s)	1292
SsiI	CCGC	3 cut(s)	105, 570, 1532
SspMI	CTAG	7 cut(s)	399, 462, 525, 705, 1020, 1293, 1650
SstI	GAGCTC	1 cut(s)	1262
StyD4I	CCNGG	8 cut(s)	21, 22, 56, 57, 901, 1053, 1071, 1213
StyI	CCWWGG	5 cut(s)	171, 524, 1191, 1310, 1635
TaaI	ACNGT	2 cut(s)	44, 1277
TaiI	ACGT	1 cut(s)	519
TaqI	TCGA	2 cut(s)	53, 110
TatI	WGTACW	3 cut(s)	39, 44, 235
TfiI	GAWTC	3 cut(s)	595, 914, 974
Tru1I	TTAA	9 cut(s)	380, 740, 831, 1155, 1371, 1385, 1398, 1554, 1623
Tru9I	TTAA	9 cut(s)	380, 740, 831, 1155, 1371, 1385, 1398, 1554, 1623
TscAI	CASTG	2 cut(s)	939, 1663
TseFI	GTSAC	1 cut(s)	26
TseI	GCWGC	3 cut(s)	71, 815, 962
Tsp45I	GTSAC	1 cut(s)	26
TspDTI	ATGAA	7 cut(s)	182, 201, 323, 420, 587, 1041, 1539
TspGWI	ACGGA	2 cut(s)	314, 731
TspMI	CCCGGG	2 cut(s)	22, 57
TspRI	CASTG	2 cut(s)	939, 1663
Van91I	CCANNNNNTGG	1 cut(s)	950
VpaK11BI	GGWCC	1 cut(s)	866
XagI	CCTNNNNNAGG	1 cut(s)	873
XapI	RAATTY	4 cut(s)	272, 884, 1387, 1599
XbaI	TCTAGA	2 cut(s)	398, 704
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1079
XmaI	CCCGGG	2 cut(s)	22, 57
XmaJI	CCTAGG	1 cut(s)	524
XspI	CTAG	7 cut(s)	399, 462, 525, 705, 1020, 1293, 1650
ZrmI	AGTACT	1 cut(s)	237
Zsp2I	ATGCAT	2 cut(s)	1095, 1210

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.