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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT1G16630 AT2G16270 |
| fragaria_vesca | FvH4_2g03070 FvH4_6g23772 |
| prunus_persica | Prupe.8G151500_v2.0.a1 Prupe.8G151700_v2.0.a1 |
| rosa_chinensis | RchiOBHm_Chr6g0247921 |
| rosa_laevigata | RLG00000015172 |
| rosa_multiflora | Rmu_co8497843.1_g000001 Rmu_sc0011977.1_g000002 Rmu_sc0017619.1_g000005 |
| rosa_roxburghii | Rroxscaffold_180G00433180 Rroxscaffold_7G00213580 |
| rosa_rugosa | Rorug05G0529500 |
| rosa_samantha | Rh6AG044300 Rh6BG039100 Rh6CG037900 Rh6DG034900 |
| rosa_wichuraiana | Rw6G003850 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1173 |
| Acc65I | GGTACC | 3 cut(s) | 403, 898, 2025 |
| AccB1I | GGYRCC | 3 cut(s) | 403, 898, 2025 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 1469, 2144 |
| AccBSI | CCGCTC | 1 cut(s) | 2031 |
| AccII | CGCG | 4 cut(s) | 8, 35, 997, 1058 |
| AccIII | TCCGGA | 1 cut(s) | 351 |
| AclWI | GGATC | 2 cut(s) | 662, 1571 |
| AcoI | YGGCCR | 1 cut(s) | 3 |
| AcsI | RAATTY | 5 cut(s) | 265, 1150, 1583, 1636, 2169 |
| AcuI | CTGAAG | 5 cut(s) | 510, 870, 1446, 1646, 1704 |
| AfaI | GTAC | 6 cut(s) | 405, 900, 1431, 1569, 1710, 2027 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 250, 430, 457, 1469, 2130, 2144 |
| AflIII | ACRYGT | 1 cut(s) | 1492 |
| AgeI | ACCGGT | 1 cut(s) | 406 |
| AjnI | CCWGG | 3 cut(s) | 692, 2067, 2081 |
| Alw21I | GWGCWC | 2 cut(s) | 164, 1980 |
| Alw26I | GTCTC | 4 cut(s) | 134, 170, 1522, 2094 |
| AlwI | GGATC | 2 cut(s) | 662, 1571 |
| Ama87I | CYCGRG | 4 cut(s) | 39, 565, 724, 1197 |
| Aor13HI | TCCGGA | 1 cut(s) | 351 |
| AoxI | GGCC | 8 cut(s) | 3, 439, 503, 695, 716, 1143, 1156, 2079 |
| ApaI | GGGCCC | 2 cut(s) | 443, 1160 |
| ApeKI | GCWGC | 4 cut(s) | 590, 1337, 1391, 1946 |
| ApoI | RAATTY | 5 cut(s) | 265, 1150, 1583, 1636, 2169 |
| AsiGI | ACCGGT | 1 cut(s) | 406 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 266, 1659 |
| Asp718I | GGTACC | 3 cut(s) | 403, 898, 2025 |
| AspLEI | GCGC | 4 cut(s) | 10, 631, 999, 1051 |
| AspS9I | GGNCC | 8 cut(s) | 241, 439, 440, 503, 1143, 1156, 1157, 2079 |
| AsuC2I | CCSGG | 2 cut(s) | 239, 802 |
| AsuHPI | GGTGA | 7 cut(s) | 88, 767, 1171, 1265, 1822, 1884, 1942 |
| AvaI | CYCGRG | 4 cut(s) | 39, 565, 724, 1197 |
| AvaII | GGWCC | 1 cut(s) | 241 |
| BaeGI | GKGCMC | 2 cut(s) | 443, 1160 |
| BanI | GGYRCC | 3 cut(s) | 403, 898, 2025 |
| BanII | GRGCYC | 4 cut(s) | 164, 348, 443, 1160 |
| BauI | CACGAG | 1 cut(s) | 1003 |
| Bbv12I | GWGCWC | 2 cut(s) | 164, 1980 |
| BbvI | GCAGC | 4 cut(s) | 602, 1349, 1403, 1933 |
| BccI | CCATC | 7 cut(s) | 290, 655, 746, 932, 1180, 1871, 2146 |
| BceAI | ACGGC | 1 cut(s) | 170 |
| BciT130I | CCWGG | 3 cut(s) | 694, 2069, 2083 |
| BciVI | GTATCC | 1 cut(s) | 781 |
| BclI | TGATCA | 3 cut(s) | 1360, 1539, 1702 |
| BcnI | CCSGG | 2 cut(s) | 239, 802 |
| BcoDI | GTCTC | 4 cut(s) | 134, 170, 1522, 2094 |
| BfuI | GTATCC | 1 cut(s) | 781 |
| BglII | AGATCT | 1 cut(s) | 324 |
| Bme1390I | CCNGG | 5 cut(s) | 239, 694, 802, 2069, 2083 |
| Bme18I | GGWCC | 1 cut(s) | 241 |
| BmeT110I | CYCGRG | 4 cut(s) | 39, 565, 724, 1197 |
| BmgT120I | GGNCC | 8 cut(s) | 241, 439, 440, 503, 1143, 1156, 1157, 2079 |
| BmrFI | CCNGG | 5 cut(s) | 239, 694, 802, 2069, 2083 |
| BmsI | GCATC | 3 cut(s) | 628, 1183, 2057 |
| BpuEI | CTTGAG | 1 cut(s) | 791 |
| BpuMI | CCSGG | 2 cut(s) | 239, 802 |
| BsaJI | CCNNGG | 6 cut(s) | 152, 165, 692, 1512, 1806, 2106 |
| BsaWI | WCCGGW | 5 cut(s) | 98, 243, 351, 400, 406 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 133, 163 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 250, 430, 457, 1469, 2130, 2144 |
| Bse118I | RCCGGY | 2 cut(s) | 250, 406 |
| Bse1I | ACTGG | 2 cut(s) | 639, 1632 |
| Bse3DI | GCAATG | 3 cut(s) | 501, 624, 1160 |
| BseAI | TCCGGA | 1 cut(s) | 351 |
| BseBI | CCWGG | 3 cut(s) | 694, 2069, 2083 |
| BseDI | CCNNGG | 6 cut(s) | 152, 165, 692, 1512, 1806, 2106 |
| BseGI | GGATG | 2 cut(s) | 643, 757 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 250, 430, 457, 1469, 2130, 2144 |
| BseMI | GCAATG | 3 cut(s) | 501, 624, 1160 |
| BseMII | CTCAG | 8 cut(s) | 546, 734, 783, 1110, 1542, 1773, 1773, 1965 |
| BseNI | ACTGG | 2 cut(s) | 639, 1632 |
| BseSI | GKGCMC | 2 cut(s) | 443, 1160 |
| BseXI | GCAGC | 4 cut(s) | 602, 1349, 1403, 1933 |
| BseYI | CCCAGC | 2 cut(s) | 935, 1819 |
| Bsh1236I | CGCG | 4 cut(s) | 8, 35, 997, 1058 |
| Bsh1285I | CGRYCG | 2 cut(s) | 248, 806 |
| BshFI | GGCC | 8 cut(s) | 5, 441, 505, 697, 718, 1145, 1158, 2081 |
| BshNI | GGYRCC | 3 cut(s) | 403, 898, 2025 |
| BshTI | ACCGGT | 1 cut(s) | 406 |
| BsiEI | CGRYCG | 2 cut(s) | 248, 806 |
| BsiHKAI | GWGCWC | 2 cut(s) | 164, 1980 |
| BsiHKCI | CYCGRG | 4 cut(s) | 39, 565, 724, 1197 |
| BslFI | GGGAC | 3 cut(s) | 10, 918, 1938 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 250, 430, 457, 1469, 2130, 2144 |
| BsmAI | GTCTC | 4 cut(s) | 134, 170, 1522, 2094 |
| BsmBI | CGTCTC | 1 cut(s) | 170 |
| BsmFI | GGGAC | 3 cut(s) | 10, 918, 1938 |
| BsmI | GAATGC | 1 cut(s) | 1448 |
| BsnI | GGCC | 8 cut(s) | 5, 441, 505, 697, 718, 1145, 1158, 2081 |
| BsoBI | CYCGRG | 4 cut(s) | 39, 565, 724, 1197 |
| Bsp120I | GGGCCC | 2 cut(s) | 439, 1156 |
| Bsp1286I | GDGCHC | 5 cut(s) | 164, 348, 443, 1160, 1980 |
| Bsp13I | TCCGGA | 1 cut(s) | 351 |
| Bsp1407I | TGTACA | 1 cut(s) | 1708 |
| Bsp143I | GATC | 9 cut(s) | 300, 324, 667, 1010, 1222, 1360, 1539, 1576, 1702 |
| Bsp19I | CCATGG | 3 cut(s) | 165, 1806, 2106 |
| Bsp68I | TCGCGA | 1 cut(s) | 35 |
| BspANI | GGCC | 8 cut(s) | 5, 441, 505, 697, 718, 1145, 1158, 2081 |
| BspCNI | CTCAG | 8 cut(s) | 545, 735, 784, 1111, 1543, 1772, 1774, 1966 |
| BspEI | TCCGGA | 1 cut(s) | 351 |
| BspFNI | CGCG | 4 cut(s) | 8, 35, 997, 1058 |
| BspHI | TCATGA | 1 cut(s) | 2198 |
| BspPI | GGATC | 2 cut(s) | 662, 1571 |
| BspT107I | GGYRCC | 3 cut(s) | 403, 898, 2025 |
| BsrBI | CCGCTC | 1 cut(s) | 2031 |
| BsrDI | GCAATG | 3 cut(s) | 501, 624, 1160 |
| BsrFI | RCCGGY | 2 cut(s) | 250, 406 |
| BsrGI | TGTACA | 1 cut(s) | 1708 |
| BsrI | ACTGG | 2 cut(s) | 639, 1632 |
| BssAI | RCCGGY | 2 cut(s) | 250, 406 |
| BssECI | CCNNGG | 6 cut(s) | 152, 165, 692, 1512, 1806, 2106 |
| BssMI | GATC | 9 cut(s) | 300, 324, 667, 1010, 1222, 1360, 1539, 1576, 1702 |
| BssSI | CACGAG | 1 cut(s) | 1003 |
| BssT1I | CCWWGG | 4 cut(s) | 165, 1512, 1806, 2106 |
| Bst2BI | CACGAG | 1 cut(s) | 1003 |
| Bst2UI | CCWGG | 3 cut(s) | 694, 2069, 2083 |
| Bst4CI | ACNGT | 5 cut(s) | 181, 551, 702, 1796, 1988 |
| Bst6I | CTCTTC | 4 cut(s) | 975, 1446, 1611, 2069 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1913 |
| BstAUI | TGTACA | 1 cut(s) | 1708 |
| BstC8I | GCNNGC | 6 cut(s) | 46, 252, 921, 992, 1099, 1735 |
| BstDEI | CTNAG | 9 cut(s) | 471, 532, 743, 792, 1119, 1551, 1759, 1782, 1974 |
| BstDSI | CCRYGG | 4 cut(s) | 152, 165, 1806, 2106 |
| BstEII | GGTNACC | 1 cut(s) | 1930 |
| BstF5I | GGATG | 2 cut(s) | 643, 757 |
| BstFNI | CGCG | 4 cut(s) | 8, 35, 997, 1058 |
| BstHHI | GCGC | 4 cut(s) | 10, 631, 999, 1051 |
| BstKTI | GATC | 9 cut(s) | 303, 327, 670, 1013, 1225, 1363, 1542, 1579, 1705 |
| BstMAI | GTCTC | 4 cut(s) | 134, 170, 1522, 2094 |
| BstMBI | GATC | 9 cut(s) | 300, 324, 667, 1010, 1222, 1360, 1539, 1576, 1702 |
| BstMCI | CGRYCG | 2 cut(s) | 248, 806 |
| BstNI | CCWGG | 3 cut(s) | 694, 2069, 2083 |
| BstNSI | RCATGY | 1 cut(s) | 1496 |
| BstPI | GGTNACC | 1 cut(s) | 1930 |
| BstSCI | CCNGG | 5 cut(s) | 237, 692, 800, 2067, 2081 |
| BstSLI | GKGCMC | 2 cut(s) | 443, 1160 |
| BstUI | CGCG | 4 cut(s) | 8, 35, 997, 1058 |
| BstV1I | GCAGC | 4 cut(s) | 602, 1349, 1403, 1933 |
| BstX2I | RGATCY | 1 cut(s) | 324 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1153 |
| BstYI | RGATCY | 1 cut(s) | 324 |
| BsuI | GTATCC | 1 cut(s) | 781 |
| BsuRI | GGCC | 8 cut(s) | 5, 441, 505, 697, 718, 1145, 1158, 2081 |
| BtgI | CCRYGG | 4 cut(s) | 152, 165, 1806, 2106 |
| BtsCI | GGATG | 2 cut(s) | 643, 757 |
| BtsI | GCAGTG | 1 cut(s) | 1020 |
| BtsIMutI | CAGTG | 5 cut(s) | 632, 1020, 1542, 1625, 1971 |
| BtuMI | TCGCGA | 1 cut(s) | 35 |
| Cac8I | GCNNGC | 6 cut(s) | 46, 252, 921, 992, 1099, 1735 |
| CciI | TCATGA | 1 cut(s) | 2198 |
| CfoI | GCGC | 4 cut(s) | 10, 631, 999, 1051 |
| Cfr10I | RCCGGY | 2 cut(s) | 250, 406 |
| Cfr13I | GGNCC | 8 cut(s) | 241, 439, 440, 503, 1143, 1156, 1157, 2079 |
| Csp6I | GTAC | 6 cut(s) | 404, 899, 1430, 1568, 1709, 2026 |
| CspAI | ACCGGT | 1 cut(s) | 406 |
| CviAII | CATG | 9 cut(s) | 166, 271, 1493, 1612, 1706, 1807, 1873, 2107, 2199 |
| CviQI | GTAC | 6 cut(s) | 404, 899, 1430, 1568, 1709, 2026 |
| DdeI | CTNAG | 9 cut(s) | 471, 532, 743, 792, 1119, 1551, 1759, 1782, 1974 |
| DpnI | GATC | 9 cut(s) | 302, 326, 669, 1012, 1224, 1362, 1541, 1578, 1704 |
| DpnII | GATC | 9 cut(s) | 300, 324, 667, 1010, 1222, 1360, 1539, 1576, 1702 |
| EaeI | YGGCCR | 1 cut(s) | 3 |
| Eam1104I | CTCTTC | 4 cut(s) | 975, 1446, 1611, 2069 |
| EarI | CTCTTC | 4 cut(s) | 975, 1446, 1611, 2069 |
| EciI | GGCGGA | 2 cut(s) | 172, 277 |
| Ecl136II | GAGCTC | 1 cut(s) | 162 |
| Eco130I | CCWWGG | 4 cut(s) | 165, 1512, 1806, 2106 |
| Eco24I | GRGCYC | 4 cut(s) | 164, 348, 443, 1160 |
| Eco47I | GGWCC | 1 cut(s) | 241 |
| Eco53kI | GAGCTC | 1 cut(s) | 162 |
| Eco57I | CTGAAG | 5 cut(s) | 510, 870, 1446, 1646, 1704 |
| Eco88I | CYCGRG | 4 cut(s) | 39, 565, 724, 1197 |
| Eco91I | GGTNACC | 1 cut(s) | 1930 |
| EcoICRI | GAGCTC | 1 cut(s) | 162 |
| EcoO109I | RGGNCCY | 1 cut(s) | 440 |
| EcoO65I | GGTNACC | 1 cut(s) | 1930 |
| EcoRI | GAATTC | 2 cut(s) | 1636, 2169 |
| EcoRII | CCWGG | 3 cut(s) | 692, 2067, 2081 |
| EcoT14I | CCWWGG | 4 cut(s) | 165, 1512, 1806, 2106 |
| EcoT38I | GRGCYC | 4 cut(s) | 164, 348, 443, 1160 |
| ErhI | CCWWGG | 4 cut(s) | 165, 1512, 1806, 2106 |
| Esp3I | CGTCTC | 1 cut(s) | 170 |
| FaeI | CATG | 9 cut(s) | 169, 274, 1496, 1615, 1709, 1810, 1876, 2110, 2202 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 311, 343, 983, 1015 |
| FaqI | GGGAC | 3 cut(s) | 10, 918, 1938 |
| FatI | CATG | 9 cut(s) | 165, 270, 1492, 1611, 1705, 1806, 1872, 2106, 2198 |
| FauI | CCCGC | 1 cut(s) | 928 |
| FauNDI | CATATG | 1 cut(s) | 2161 |
| FbaI | TGATCA | 3 cut(s) | 1360, 1539, 1702 |
| FokI | GGATG | 2 cut(s) | 650, 764 |
| FriOI | GRGCYC | 4 cut(s) | 164, 348, 443, 1160 |
| GlaI | GCGC | 4 cut(s) | 9, 630, 998, 1050 |
| GsaI | CCCAGC | 2 cut(s) | 939, 1823 |
| HaeIII | GGCC | 8 cut(s) | 5, 441, 505, 697, 718, 1145, 1158, 2081 |
| HhaI | GCGC | 4 cut(s) | 10, 631, 999, 1051 |
| Hin1II | CATG | 9 cut(s) | 169, 274, 1496, 1615, 1709, 1810, 1876, 2110, 2202 |
| Hin6I | GCGC | 4 cut(s) | 8, 629, 997, 1049 |
| HinP1I | GCGC | 4 cut(s) | 8, 629, 997, 1049 |
| HincII | GTYRAC | 1 cut(s) | 1477 |
| HindII | GTYRAC | 1 cut(s) | 1477 |
| HindIII | AAGCTT | 2 cut(s) | 91, 1302 |
| HphI | GGTGA | 7 cut(s) | 88, 767, 1171, 1265, 1822, 1884, 1942 |
| Hpy166II | GTNNAC | 2 cut(s) | 1020, 1477 |
| Hpy8I | GTNNAC | 2 cut(s) | 1020, 1477 |
| Hpy99I | CGWCG | 2 cut(s) | 19, 131 |
| HpyAV | CCTTC | 5 cut(s) | 129, 814, 1905, 2001, 2176 |
| HpyCH4III | ACNGT | 5 cut(s) | 181, 551, 702, 1796, 1988 |
| HpyCH4V | TGCA | 7 cut(s) | 1097, 1165, 1324, 1403, 1733, 1907, 1949 |
| HpyF3I | CTNAG | 9 cut(s) | 471, 532, 743, 792, 1119, 1551, 1759, 1782, 1974 |
| Hsp92II | CATG | 9 cut(s) | 169, 274, 1496, 1615, 1709, 1810, 1876, 2110, 2202 |
| HspAI | GCGC | 4 cut(s) | 8, 629, 997, 1049 |
| Kpn2I | TCCGGA | 1 cut(s) | 351 |
| KpnI | GGTACC | 3 cut(s) | 407, 902, 2029 |
| KroI | GCCGGC | 1 cut(s) | 250 |
| KroNI | GCCGGC | 1 cut(s) | 252 |
| Ksp22I | TGATCA | 3 cut(s) | 1360, 1539, 1702 |
| Kzo9I | GATC | 9 cut(s) | 300, 324, 667, 1010, 1222, 1360, 1539, 1576, 1702 |
| LmnI | GCTCC | 7 cut(s) | 68, 159, 167, 403, 658, 1334, 1388 |
| Lsp1109I | GCAGC | 4 cut(s) | 602, 1349, 1403, 1933 |
| LweI | GCATC | 3 cut(s) | 628, 1183, 2057 |
| MaeIII | GTNAC | 6 cut(s) | 184, 835, 1229, 1840, 1930, 2185 |
| MalI | GATC | 9 cut(s) | 302, 326, 669, 1012, 1224, 1362, 1541, 1578, 1704 |
| MbiI | CCGCTC | 1 cut(s) | 2031 |
| MboI | GATC | 9 cut(s) | 300, 324, 667, 1010, 1222, 1360, 1539, 1576, 1702 |
| MfeI | CAATTG | 2 cut(s) | 1356, 2115 |
| MflI | RGATCY | 1 cut(s) | 324 |
| MhlI | GDGCHC | 5 cut(s) | 164, 348, 443, 1160, 1980 |
| MlyI | GAGTC | 5 cut(s) | 136, 992, 1482, 2096, 2114 |
| MmeI | TCCRAC | 4 cut(s) | 206, 444, 786, 1831 |
| MreI | CGCCGGCG | 1 cut(s) | 250 |
| MroI | TCCGGA | 1 cut(s) | 351 |
| MroNI | GCCGGC | 1 cut(s) | 250 |
| MroXI | GAANNNNTTC | 2 cut(s) | 266, 1659 |
| MseI | TTAA | 3 cut(s) | 1203, 1740, 2178 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1184, 1871 |
| MspA1I | CMGCKG | 4 cut(s) | 593, 1340, 1394, 1781 |
| MspR9I | CCNGG | 5 cut(s) | 239, 694, 802, 2069, 2083 |
| MunI | CAATTG | 2 cut(s) | 1356, 2115 |
| Mva1269I | GAATGC | 1 cut(s) | 1448 |
| MvaI | CCWGG | 3 cut(s) | 694, 2069, 2083 |
| MvnI | CGCG | 4 cut(s) | 8, 35, 997, 1058 |
| NaeI | GCCGGC | 1 cut(s) | 252 |
| NciI | CCSGG | 2 cut(s) | 239, 802 |
| NcoI | CCATGG | 3 cut(s) | 165, 1806, 2106 |
| NdeI | CATATG | 1 cut(s) | 2161 |
| NdeII | GATC | 9 cut(s) | 300, 324, 667, 1010, 1222, 1360, 1539, 1576, 1702 |
| NgoMIV | GCCGGC | 1 cut(s) | 250 |
| NlaIII | CATG | 9 cut(s) | 169, 274, 1496, 1615, 1709, 1810, 1876, 2110, 2202 |
| NmuCI | GTSAC | 3 cut(s) | 1229, 1840, 1930 |
| NruI | TCGCGA | 1 cut(s) | 35 |
| NspI | RCATGY | 1 cut(s) | 1496 |
| PagI | TCATGA | 1 cut(s) | 2198 |
| PciI | ACATGT | 1 cut(s) | 1492 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 78 |
| PctI | GAATGC | 1 cut(s) | 1448 |
| PdiI | GCCGGC | 1 cut(s) | 252 |
| PdmI | GAANNNNTTC | 2 cut(s) | 266, 1659 |
| PfeI | GAWTC | 5 cut(s) | 218, 556, 1601, 1771, 1854 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 1469, 2144 |
| PinAI | ACCGGT | 1 cut(s) | 406 |
| PleI | GAGTC | 5 cut(s) | 136, 991, 1481, 2096, 2114 |
| PpsI | GAGTC | 5 cut(s) | 136, 991, 1481, 2096, 2114 |
| PscI | ACATGT | 1 cut(s) | 1492 |
| PsiI | TTATAA | 1 cut(s) | 1173 |
| Psp124BI | GAGCTC | 1 cut(s) | 164 |
| Psp6I | CCWGG | 3 cut(s) | 692, 2067, 2081 |
| PspEI | GGTNACC | 1 cut(s) | 1930 |
| PspFI | CCCAGC | 2 cut(s) | 935, 1819 |
| PspGI | CCWGG | 3 cut(s) | 692, 2067, 2081 |
| PspOMI | GGGCCC | 2 cut(s) | 439, 1156 |
| PspPI | GGNCC | 8 cut(s) | 241, 439, 440, 503, 1143, 1156, 1157, 2079 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 1202, 1234 |
| PsuI | RGATCY | 1 cut(s) | 324 |
| PvuII | CAGCTG | 3 cut(s) | 1340, 1394, 1781 |
| RruI | TCGCGA | 1 cut(s) | 35 |
| RsaI | GTAC | 6 cut(s) | 405, 900, 1431, 1569, 1710, 2027 |
| RsaNI | GTAC | 6 cut(s) | 404, 899, 1430, 1568, 1709, 2026 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1184, 1871 |
| SacI | GAGCTC | 1 cut(s) | 164 |
| SaqAI | TTAA | 3 cut(s) | 1203, 1740, 2178 |
| Sau3AI | GATC | 9 cut(s) | 300, 324, 667, 1010, 1222, 1360, 1539, 1576, 1702 |
| Sau96I | GGNCC | 8 cut(s) | 241, 439, 440, 503, 1143, 1156, 1157, 2079 |
| SchI | GAGTC | 5 cut(s) | 136, 992, 1482, 2096, 2114 |
| ScrFI | CCNGG | 5 cut(s) | 239, 694, 802, 2069, 2083 |
| SduI | GDGCHC | 5 cut(s) | 164, 348, 443, 1160, 1980 |
| SfaNI | GCATC | 3 cut(s) | 628, 1183, 2057 |
| SgrAI | CRCCGGYG | 1 cut(s) | 250 |
| SinI | GGWCC | 1 cut(s) | 241 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1184, 1871 |
| SmlI | CTYRAG | 1 cut(s) | 770 |
| SmoI | CTYRAG | 1 cut(s) | 770 |
| SspI | AATATT | 5 cut(s) | 416, 812, 1169, 1504, 1693 |
| SstI | GAGCTC | 1 cut(s) | 164 |
| StyD4I | CCNGG | 5 cut(s) | 237, 692, 800, 2067, 2081 |
| StyI | CCWWGG | 4 cut(s) | 165, 1512, 1806, 2106 |
| TaaI | ACNGT | 5 cut(s) | 181, 551, 702, 1796, 1988 |
| TaqI | TCGA | 3 cut(s) | 17, 221, 1221 |
| TaqII | GACCGA | 2 cut(s) | 347, 1949 |
| TatI | WGTACW | 2 cut(s) | 1567, 1708 |
| TauI | GCSGC | 8 cut(s) | 8, 13, 257, 260, 293, 596, 599, 997 |
| TfiI | GAWTC | 5 cut(s) | 218, 556, 1601, 1771, 1854 |
| Tru1I | TTAA | 3 cut(s) | 1203, 1740, 2178 |
| Tru9I | TTAA | 3 cut(s) | 1203, 1740, 2178 |
| TscAI | CASTG | 5 cut(s) | 639, 1027, 1542, 1632, 1978 |
| TseFI | GTSAC | 3 cut(s) | 1229, 1840, 1930 |
| TseI | GCWGC | 4 cut(s) | 590, 1337, 1391, 1946 |
| Tsp45I | GTSAC | 3 cut(s) | 1229, 1840, 1930 |
| TspDTI | ATGAA | 8 cut(s) | 259, 698, 1114, 1362, 1659, 1889, 2070, 2187 |
| TspRI | CASTG | 5 cut(s) | 639, 1027, 1542, 1632, 1978 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 1469, 2144 |
| VpaK11BI | GGWCC | 1 cut(s) | 241 |
| XapI | RAATTY | 5 cut(s) | 265, 1150, 1583, 1636, 2169 |
| XceI | RCATGY | 1 cut(s) | 1496 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1466 |
| XmnI | GAANNNNTTC | 2 cut(s) | 266, 1659 |