Rh6CG014400

Nodulin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0003876
Physical Location & Seq
Forward (+)
1576832 .. 1580910
4079 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG014400.1

Sequence Viewer

Length: 1242 bp
ATGGGTTTTGGTTTGGAGCGACCTTCTGTTTCATCAACCATCAAATGGCTTGGTTTCGTCACCGCCATTTGGGTCCAGTCCATTTCAGGCAACAACTACACTTTCTCCAACTACTCCGGTGCTCTCAAAACCCTCATGAACCTCACCCAGCTCGAGCTCAACAACCTCTCCGTCGCCAAAGACGTCGGTAAAGCTTTCGGCCTCCTCGCGGGCCTCGCTTCCGACCGCTTCCCCACCTGGGTCCTCCTCCTCATCGGCTCCGTCGAGGGCTTGGTCGGTTACGGCGCCCAGTGGCTCGTCGTCAGCCAAAAAATTCAACCCCTTTCGTACTGGCAGATGTGCATATTTCTTTGCATGGGAGGCAACAGCACCACGTGGATGAACACGGCCGTATTAGTGACGTGTATTCGGAACTTCCGGAGCAACCGAGGACCGGTCTCCGGCATTCTCAAAGGCTACGTGGGTCTGAGCACCGCCATATTCACCGACCTCTGCACCGCTCTGTTTAACGACAACTCGGCCAGCTTCCTGTTCATGCTCGCCGTCGCTCCCTTCGCCGTCTGCCTCACCGCCATGGTCTTCCTCCGCGAAATCCCTCCGTCGTCCTCCGCCGCCGAGGACAAGCAGGAGTCTCGATACTTCTGGTTCTTCAACGGCCTCGCTTTCTTCGTCGCCCTCTACCTCTTGGCTTACGACTTCATTCCAGACCCCTCCACGTTCTTCTCTTACGTCTTCTCCGCCACTCTCTTACTCCTCCTCGCTTCGCCGCTGGTGGTTCCGGCCTACTCATATTTCAAGGCGTGGGCCTCGAACCGGGTCGGGTCGTCGGATCCGGAAAAGCCGGTTGCGGTTTTGGTTCTGGACGAGCCGCTGCTGGCGGCGGAAAAGCCGGAGGTGGTACCGGCGGAGGAGGAGGGTGACGTGGCGGTGGTGGAGAGGAGGAGGAGGAGGCCGGTGATCGGAGAGGAGCACACGATTTTCGAGGCGATGAGGACTTTGGATTTCTGGATACTTTTTGTGTCGTTTCTGTGTGGGGTGGGGACTGGATTGGCGGTTATGAACAATTTGGGCCAGATTGGGCTGGCGCTTGGATACGCCGACGTTTCGATCTTCGTTTCGCTCACCAGTATTTGGGGGTTTTTCGGGCGAATCGTTTCGGGTATGGTTTCCGAGTATTTCATCAGGTCAGTTACCCATTCCCCTTGCTTTCACCTCAATAAATTATTATTTAATGGATATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nodulin-like

Protein Analysis

413

Amino Acids

45.48

Weight (kDa)

5.73

Isoelectric Point (pI)

32.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nodulin-like PF06813 15 - 260 2.6e-94 Nodulin-like
NFD4_C PF23262 322 - 396 5.2e-11 NFD4, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 186
Acc65I GGTACC 1 cut(s) 898
AccB1I GGYRCC 2 cut(s) 284, 898
AccB7I CCANNNNNTGG 2 cut(s) 45, 1131
AccBSI CCGCTC 1 cut(s) 500
AccII CGCG 2 cut(s) 209, 588
AccIII TCCGGA 2 cut(s) 417, 832
AclWI GGATC 2 cut(s) 824, 837
AcoI YGGCCR 2 cut(s) 387, 519
AcsI RAATTY 1 cut(s) 312
AcvI CACGTG 1 cut(s) 375
AcyI GRCGYC 2 cut(s) 183, 285
AdeI CACNNNGTG 1 cut(s) 375
AfaI GTAC 2 cut(s) 329, 900
AfiI CCNNNNNNNGG 9 cut(s) 45, 69, 208, 238, 433, 440, 813, 959, 1131
AflIII ACRYGT 1 cut(s) 401
AgeI ACCGGT 1 cut(s) 433
AgsI TTSAA 3 cut(s) 317, 652, 796
AjiI CACGTC 2 cut(s) 402, 922
AjnI CCWGG 1 cut(s) 236
AluBI AGCT 4 cut(s) 151, 157, 194, 525
AluI AGCT 4 cut(s) 151, 157, 194, 525
Alw21I GWGCWC 4 cut(s) 124, 159, 473, 972
Alw26I GTCTC 2 cut(s) 442, 636
AlwI GGATC 2 cut(s) 824, 837
Ama87I CYCGRG 1 cut(s) 152
Aor13HI TCCGGA 2 cut(s) 417, 832
AoxI GGCC 9 cut(s) 199, 211, 387, 519, 655, 780, 804, 950, 1069
ApeKI GCWGC 1 cut(s) 871
ApoI RAATTY 1 cut(s) 312
AsiGI ACCGGT 1 cut(s) 433
Asp700I GAANNNNTTC 1 cut(s) 1153
Asp718I GGTACC 1 cut(s) 898
AspLEI GCGC 2 cut(s) 287, 1087
AspS9I GGNCC 6 cut(s) 73, 211, 241, 431, 804, 1069
AsuC2I CCSGG 1 cut(s) 815
AsuHPI GGTGA 8 cut(s) 52, 136, 475, 559, 929, 967, 1114, 1202
AvaI CYCGRG 1 cut(s) 152
AvaII GGWCC 3 cut(s) 73, 241, 431
BamHI GGATCC 1 cut(s) 829
BanI GGYRCC 2 cut(s) 284, 898
BanII GRGCYC 1 cut(s) 159
BbrPI CACGTG 1 cut(s) 375
BbsI GAAGAC 2 cut(s) 571, 724
Bbv12I GWGCWC 4 cut(s) 124, 159, 473, 972
BbvI GCAGC 1 cut(s) 858
BccI CCATC 1 cut(s) 47
BceAI ACGGC 6 cut(s) 298, 374, 402, 527, 542, 670
BcgI CGANNNNNNTGC 2 cut(s) 614, 648
BciT130I CCWGG 1 cut(s) 238
BciVI GTATCC 2 cut(s) 1002, 1085
BcnI CCSGG 1 cut(s) 815
BcoDI GTCTC 2 cut(s) 442, 636
BfoI RGCGCY 2 cut(s) 288, 1088
BfuI GTATCC 2 cut(s) 1002, 1085
BisI GCNGC 5 cut(s) 612, 767, 869, 872, 879
BlsI GCNGC 5 cut(s) 613, 768, 870, 873, 880
Bme1390I CCNGG 2 cut(s) 238, 815
Bme18I GGWCC 3 cut(s) 73, 241, 431
BmeT110I CYCGRG 1 cut(s) 152
BmgBI CACGTC 2 cut(s) 402, 922
BmgT120I GGNCC 6 cut(s) 73, 211, 241, 431, 804, 1069
BmiI GGNNCC 7 cut(s) 74, 242, 259, 286, 777, 831, 900
BmrFI CCNGG 2 cut(s) 238, 815
BmrI ACTGGG 1 cut(s) 283
BmuI ACTGGG 1 cut(s) 283
BpiI GAAGAC 2 cut(s) 571, 724
BpuMI CCSGG 1 cut(s) 815
BsaAI YACGTR 2 cut(s) 375, 460
BsaHI GRCGYC 2 cut(s) 183, 285
BsaI GGTCTC 1 cut(s) 442
BsaJI CCNNGG 4 cut(s) 237, 427, 573, 615
BsaWI WCCGGW 4 cut(s) 116, 417, 433, 832
BsaXI ACNNNNNCTCC 6 cut(s) 89, 119, 152, 182, 719, 749
Bsc4I CCNNNNNNNGG 9 cut(s) 45, 69, 208, 238, 433, 440, 813, 959, 1131
Bse118I RCCGGY 4 cut(s) 433, 841, 901, 952
Bse1I ACTGG 5 cut(s) 76, 289, 335, 1048, 1125
BseAI TCCGGA 2 cut(s) 417, 832
BseBI CCWGG 1 cut(s) 238
BseDI CCNNGG 4 cut(s) 237, 427, 573, 615
BseGI GGATG 1 cut(s) 384
BseLI CCNNNNNNNGG 9 cut(s) 45, 69, 208, 238, 433, 440, 813, 959, 1131
BseMII CTCAG 1 cut(s) 458
BseNI ACTGG 5 cut(s) 76, 289, 335, 1048, 1125
BseX3I CGGCCG 1 cut(s) 387
BseXI GCAGC 1 cut(s) 858
BseYI CCCAGC 1 cut(s) 147
BsgI GTGCAG 1 cut(s) 478
Bsh1236I CGCG 2 cut(s) 209, 588
Bsh1285I CGRYCG 2 cut(s) 226, 390
BshFI GGCC 9 cut(s) 201, 213, 389, 521, 657, 782, 806, 952, 1071
BshNI GGYRCC 2 cut(s) 284, 898
BshTI ACCGGT 1 cut(s) 433
BsiEI CGRYCG 2 cut(s) 226, 390
BsiHKAI GWGCWC 4 cut(s) 124, 159, 473, 972
BsiHKCI CYCGRG 1 cut(s) 152
BslFI GGGAC 1 cut(s) 1054
BslI CCNNNNNNNGG 9 cut(s) 45, 69, 208, 238, 433, 440, 813, 959, 1131
BsmAI GTCTC 2 cut(s) 442, 636
BsmFI GGGAC 1 cut(s) 1054
BsmI GAATGC 1 cut(s) 444
BsnI GGCC 9 cut(s) 201, 213, 389, 521, 657, 782, 806, 952, 1071
Bso31I GGTCTC 1 cut(s) 442
BsoBI CYCGRG 1 cut(s) 152
Bsp1286I GDGCHC 4 cut(s) 124, 159, 473, 972
Bsp13I TCCGGA 2 cut(s) 417, 832
Bsp143I GATC 3 cut(s) 829, 957, 1107
Bsp19I CCATGG 1 cut(s) 573
BspANI GGCC 9 cut(s) 201, 213, 389, 521, 657, 782, 806, 952, 1071
BspCNI CTCAG 1 cut(s) 459
BspEI TCCGGA 2 cut(s) 417, 832
BspFNI CGCG 2 cut(s) 209, 588
BspHI TCATGA 1 cut(s) 135
BspLI GGNNCC 7 cut(s) 74, 242, 259, 286, 777, 831, 900
BspPI GGATC 2 cut(s) 824, 837
BspT107I GGYRCC 2 cut(s) 284, 898
BspTNI GGTCTC 1 cut(s) 442
BsrBI CCGCTC 1 cut(s) 500
BsrFI RCCGGY 4 cut(s) 433, 841, 901, 952
BsrI ACTGG 5 cut(s) 76, 289, 335, 1048, 1125
BssAI RCCGGY 4 cut(s) 433, 841, 901, 952
BssECI CCNNGG 4 cut(s) 237, 427, 573, 615
BssMI GATC 3 cut(s) 829, 957, 1107
BssNI GRCGYC 2 cut(s) 183, 285
BssT1I CCWWGG 1 cut(s) 573
Bst2UI CCWGG 1 cut(s) 238
BstACI GRCGYC 2 cut(s) 183, 285
BstBAI YACGTR 2 cut(s) 375, 460
BstC8I GCNNGC 5 cut(s) 211, 523, 540, 876, 1083
BstDEI CTNAG 1 cut(s) 467
BstDSI CCRYGG 1 cut(s) 573
BstF5I GGATG 1 cut(s) 384
BstFNI CGCG 2 cut(s) 209, 588
BstH2I RGCGCY 2 cut(s) 288, 1088
BstHHI GCGC 2 cut(s) 287, 1087
BstKTI GATC 3 cut(s) 832, 960, 1110
BstMAI GTCTC 2 cut(s) 442, 636
BstMBI GATC 3 cut(s) 829, 957, 1107
BstMCI CGRYCG 2 cut(s) 226, 390
BstMWI GCNNNNNNNGC 3 cut(s) 215, 360, 554
BstNI CCWGG 1 cut(s) 238
BstSCI CCNGG 2 cut(s) 236, 813
BstUI CGCG 2 cut(s) 209, 588
BstV1I GCAGC 1 cut(s) 858
BstV2I GAAGAC 2 cut(s) 571, 724
BstX2I RGATCY 1 cut(s) 829
BstYI RGATCY 1 cut(s) 829
BstZI CGGCCG 1 cut(s) 387
BsuI GTATCC 2 cut(s) 1002, 1085
BsuRI GGCC 9 cut(s) 201, 213, 389, 521, 657, 782, 806, 952, 1071
BtgI CCRYGG 1 cut(s) 573
BtgZI GCGATG 1 cut(s) 1001
BtrI CACGTC 2 cut(s) 402, 922
BtsCI GGATG 1 cut(s) 384
BtsIMutI CAGTG 1 cut(s) 296
Cac8I GCNNGC 5 cut(s) 211, 523, 540, 876, 1083
CciI TCATGA 1 cut(s) 135
CfoI GCGC 2 cut(s) 287, 1087
Cfr10I RCCGGY 4 cut(s) 433, 841, 901, 952
Cfr13I GGNCC 6 cut(s) 73, 211, 241, 431, 804, 1069
Csp6I GTAC 2 cut(s) 328, 899
CspAI ACCGGT 1 cut(s) 433
CviAII CATG 4 cut(s) 136, 355, 535, 574
CviQI GTAC 2 cut(s) 328, 899
DdeI CTNAG 1 cut(s) 467
DinI GGCGCC 1 cut(s) 286
DpnI GATC 3 cut(s) 831, 959, 1109
DpnII GATC 3 cut(s) 829, 957, 1107
DraIII CACNNNGTG 1 cut(s) 375
EaeI YGGCCR 2 cut(s) 387, 519
EagI CGGCCG 1 cut(s) 387
EciI GGCGGA 4 cut(s) 598, 727, 896, 920
Ecl136II GAGCTC 1 cut(s) 157
EclXI CGGCCG 1 cut(s) 387
Eco130I CCWWGG 1 cut(s) 573
Eco24I GRGCYC 1 cut(s) 159
Eco31I GGTCTC 1 cut(s) 442
Eco47I GGWCC 3 cut(s) 73, 241, 431
Eco52I CGGCCG 1 cut(s) 387
Eco53kI GAGCTC 1 cut(s) 157
Eco72I CACGTG 1 cut(s) 375
Eco88I CYCGRG 1 cut(s) 152
EcoICRI GAGCTC 1 cut(s) 157
EcoO109I RGGNCCY 1 cut(s) 241
EcoRII CCWGG 1 cut(s) 236
EcoT14I CCWWGG 1 cut(s) 573
EcoT38I GRGCYC 1 cut(s) 159
EgeI GGCGCC 1 cut(s) 286
EheI GGCGCC 1 cut(s) 286
ErhI CCWWGG 1 cut(s) 573
FaeI CATG 4 cut(s) 139, 358, 538, 577
FaiI YATR 9 cut(s) 137, 344, 356, 479, 536, 575, 790, 1058, 1163
FaqI GGGAC 1 cut(s) 1054
FatI CATG 4 cut(s) 135, 354, 534, 573
FauI CCCGC 1 cut(s) 202
Fnu4HI GCNGC 5 cut(s) 612, 767, 869, 872, 879
FokI GGATG 1 cut(s) 391
FriOI GRGCYC 1 cut(s) 159
Fsp4HI GCNGC 5 cut(s) 612, 767, 869, 872, 879
GlaI GCGC 2 cut(s) 286, 1086
GluI GCNGC 5 cut(s) 612, 767, 869, 872, 879
GsaI CCCAGC 1 cut(s) 151
HaeII RGCGCY 2 cut(s) 288, 1088
HaeIII GGCC 9 cut(s) 201, 213, 389, 521, 657, 782, 806, 952, 1071
HhaI GCGC 2 cut(s) 287, 1087
Hin1I GRCGYC 2 cut(s) 183, 285
Hin1II CATG 4 cut(s) 139, 358, 538, 577
Hin6I GCGC 2 cut(s) 285, 1085
HinP1I GCGC 2 cut(s) 285, 1085
HindIII AAGCTT 1 cut(s) 192
HinfI GANTC 2 cut(s) 629, 1149
HphI GGTGA 8 cut(s) 52, 136, 475, 559, 929, 967, 1114, 1202
Hpy188I TCNGA 6 cut(s) 223, 411, 468, 829, 962, 1171
Hpy188III TCNNGA 7 cut(s) 136, 418, 633, 704, 833, 860, 1006
Hpy99I CGWCG 9 cut(s) 176, 188, 266, 302, 548, 604, 674, 829, 1103
HpyAV CCTTC 2 cut(s) 33, 562
HpyCH4IV ACGT 8 cut(s) 183, 374, 401, 459, 716, 729, 921, 1101
HpyCH4V TGCA 3 cut(s) 342, 354, 495
HpyF10VI GCNNNNNNNGC 3 cut(s) 215, 360, 554
HpyF3I CTNAG 1 cut(s) 467
HpySE526I ACGT 8 cut(s) 183, 374, 401, 459, 716, 729, 921, 1101
Hsp92I GRCGYC 2 cut(s) 183, 285
Hsp92II CATG 4 cut(s) 139, 358, 538, 577
HspAI GCGC 2 cut(s) 285, 1085
KasI GGCGCC 1 cut(s) 284
Kpn2I TCCGGA 2 cut(s) 417, 832
KpnI GGTACC 1 cut(s) 902
Kzo9I GATC 3 cut(s) 829, 957, 1107
LmnI GCTCC 5 cut(s) 16, 263, 420, 553, 967
Lsp1109I GCAGC 1 cut(s) 858
MaeII ACGT 8 cut(s) 183, 374, 401, 459, 716, 729, 921, 1101
MaeIII GTNAC 5 cut(s) 58, 278, 397, 917, 1189
MalI GATC 3 cut(s) 831, 959, 1109
MbiI CCGCTC 1 cut(s) 500
MboI GATC 3 cut(s) 829, 957, 1107
MboII GAAGA 6 cut(s) 571, 640, 658, 712, 724, 1102
MflI RGATCY 1 cut(s) 829
MhlI GDGCHC 4 cut(s) 124, 159, 473, 972
MluCI AATT 3 cut(s) 312, 1063, 1220
Mly113I GGCGCC 1 cut(s) 285
MlyI GAGTC 1 cut(s) 638
MmeI TCCRAC 3 cut(s) 132, 246, 807
MroI TCCGGA 2 cut(s) 417, 832
MroXI GAANNNNTTC 1 cut(s) 1153
MseI TTAA 3 cut(s) 507, 1230, 1240
MslI CAYNNNNRTG 2 cut(s) 377, 572
MspA1I CMGCKG 2 cut(s) 769, 871
MspR9I CCNGG 2 cut(s) 238, 815
Mva1269I GAATGC 1 cut(s) 444
MvaI CCWGG 1 cut(s) 238
MvnI CGCG 2 cut(s) 209, 588
MwoI GCNNNNNNNGC 3 cut(s) 215, 360, 554
NarI GGCGCC 1 cut(s) 285
NciI CCSGG 1 cut(s) 815
NcoI CCATGG 1 cut(s) 573
NdeII GATC 3 cut(s) 829, 957, 1107
NlaIII CATG 4 cut(s) 139, 358, 538, 577
NlaIV GGNNCC 7 cut(s) 74, 242, 259, 286, 777, 831, 900
NmeAIII GCCGAG 2 cut(s) 497, 640
NmuCI GTSAC 3 cut(s) 58, 397, 917
PaeR7I CTCGAG 1 cut(s) 152
PagI TCATGA 1 cut(s) 135
PcsI WCGNNNNNNNCGW 4 cut(s) 180, 261, 666, 1149
PctI GAATGC 1 cut(s) 444
PdmI GAANNNNTTC 1 cut(s) 1153
PfeI GAWTC 1 cut(s) 1149
PflMI CCANNNNNTGG 2 cut(s) 45, 1131
PinAI ACCGGT 1 cut(s) 433
PkrI GCNGC 5 cut(s) 613, 768, 870, 873, 880
PleI GAGTC 1 cut(s) 637
PluTI GGCGCC 1 cut(s) 288
PmaCI CACGTG 1 cut(s) 375
PmlI CACGTG 1 cut(s) 375
PpsI GAGTC 1 cut(s) 637
Ppu21I YACGTR 2 cut(s) 375, 460
PpuMI RGGWCCY 1 cut(s) 241
Psp124BI GAGCTC 1 cut(s) 159
Psp5II RGGWCCY 1 cut(s) 241
Psp6I CCWGG 1 cut(s) 236
PspCI CACGTG 1 cut(s) 375
PspFI CCCAGC 1 cut(s) 147
PspGI CCWGG 1 cut(s) 236
PspN4I GGNNCC 7 cut(s) 74, 242, 259, 286, 777, 831, 900
PspPI GGNCC 6 cut(s) 73, 211, 241, 431, 804, 1069
PspPPI RGGWCCY 1 cut(s) 241
PspXI VCTCGAGB 1 cut(s) 152
PsuI RGATCY 1 cut(s) 829
RsaI GTAC 2 cut(s) 329, 900
RsaNI GTAC 2 cut(s) 328, 899
RseI CAYNNNNRTG 2 cut(s) 377, 572
SacI GAGCTC 1 cut(s) 159
SaqAI TTAA 3 cut(s) 507, 1230, 1240
SatI GCNGC 5 cut(s) 612, 767, 869, 872, 879
Sau3AI GATC 3 cut(s) 829, 957, 1107
Sau96I GGNCC 6 cut(s) 73, 211, 241, 431, 804, 1069
SchI GAGTC 1 cut(s) 638
ScrFI CCNGG 2 cut(s) 238, 815
SduI GDGCHC 4 cut(s) 124, 159, 473, 972
SfoI GGCGCC 1 cut(s) 286
Sfr274I CTCGAG 1 cut(s) 152
SinI GGWCC 3 cut(s) 73, 241, 431
SlaI CTCGAG 1 cut(s) 152
SmiMI CAYNNNNRTG 2 cut(s) 377, 572
SmlI CTYRAG 1 cut(s) 152
SmoI CTYRAG 1 cut(s) 152
Sse9I AATT 3 cut(s) 312, 1063, 1220
SspDI GGCGCC 1 cut(s) 284
SstI GAGCTC 1 cut(s) 159
StyD4I CCNGG 2 cut(s) 236, 813
StyI CCWWGG 1 cut(s) 573
TaiI ACGT 8 cut(s) 186, 377, 404, 462, 719, 732, 924, 1104
TaqI TCGA 6 cut(s) 153, 264, 634, 809, 981, 1106
TasI AATT 3 cut(s) 312, 1063, 1220
TauI GCSGC 4 cut(s) 614, 769, 871, 881
TfiI GAWTC 1 cut(s) 1149
Tru1I TTAA 3 cut(s) 507, 1230, 1240
Tru9I TTAA 3 cut(s) 507, 1230, 1240
TscAI CASTG 1 cut(s) 296
TseFI GTSAC 3 cut(s) 58, 397, 917
TseI GCWGC 1 cut(s) 871
Tsp45I GTSAC 3 cut(s) 58, 397, 917
TspDTI ATGAA 7 cut(s) 21, 152, 395, 523, 688, 1073, 1168
TspGWI ACGGA 3 cut(s) 160, 250, 588
TspRI CASTG 1 cut(s) 296
Van91I CCANNNNNTGG 2 cut(s) 45, 1131
VpaK11BI GGWCC 3 cut(s) 73, 241, 431
XapI RAATTY 1 cut(s) 312
XhoI CTCGAG 1 cut(s) 152
XmnI GAANNNNTTC 1 cut(s) 1153
ZraI GACGTC 1 cut(s) 184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.