Rh6CG425100

Acyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0005846
Physical Location & Seq
Reverse (-)
60783265 .. 60787313
4049 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG425100.1

Sequence Viewer

Length: 1080 bp
ATGGAATCGGAGCTCAAGGACCTGAATTCCACACCGGCATCAAAGCCCTCCTCCTCCGCCAAGGACGACCGCCCGCTCCTCAAACCCGAGCCGGCCCCGTCCGTCTCCGCCGAGGAGCTCCAGGAGCTCGAGAAGAAATGCGCCGCCTACGTCCGCAGAGACGCGTACGGCACCATGGGACGCGGCGAGCTGTCGCTCAAGGAGAAGCTCCTGCTCGGCGTCGCCCTCGTCACTCTGGTCCCGATACGCGTGGTGGTGAGCATGACGCTCCTCCTCTTCTATTACTTGATTTGTAGGATTTGCACGCTCTTCTCTTCTCCGAATCGAGACGAGGACGAGCAAGAGGACTACGCGCACATGGGAGGCTGGAGACGCGCCGTGATTGTTTGGTGCGGCCGATCCTTATCCAGAGTCATGCTCTTCGTCCTAGGGTTTTATTGGATCAGCGAAACCTATCGGATACCAGACCCTGCTCAACAATCCAACCCTGAGGGAAAAGAGGATAGTGGAGAGGCTGACAAGCCTGGGGCTATTATATCTAACCATGTATCGTATTTGGATATCTTGTATCACATGTCTTCTTCCTTTCCGAGCTTTGTTGCCAAGAGATCGGTGGCGAAACTTCCTCTAGTTGGCCTCATCAGCAAGTGCCTCGGTTGTGTGTATGTTCAGCGGGAGTCAAAGTCATCCGACTTCAAGGGTGTTGCAGGTGTTGTGACAGATAGAGTTCGAGAAGCACATCAGAATAAATCTGCACCACAAATTATGCTTTTCCCAGAAGGTACTACTACAAATGGAGACTTTCTTCTGCCATTCAAGACAGGAGCATTTTTGGCTAAAGCTCCGGTGCTTCCAGTGATTCTTAGATATCCTTACCAGAGATTTAGTCCTGCCTGGGACTCGATATCTGGGGTGCGACATGTGATATTTCTTCTCTGTCAATTTAAAAATCGCATAGAGGTCACAAGGTTACCTGTTTATTACCCTTCACAGGAAGAAAAGGATGACCCAAAACTGTACGCTAATAATGTCAGAAAATTGATGGCCAGTGAGGTATTAATTCTTAAGAGTATATTATGA

Protein Analysis

359

Amino Acids

40.27

Weight (kDa)

8.68

Isoelectric Point (pI)

51.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acyltransferase PF01553 172 - 288 2.7e-18 Acyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 698
Acc36I ACCTGC 1 cut(s) 698
AccB1I GGYRCC 1 cut(s) 170
AccBSI CCGCTC 1 cut(s) 76
AccII CGCG 5 cut(s) 164, 183, 249, 353, 375
AciI CCGC 9 cut(s) 57, 70, 74, 108, 144, 154, 183, 393, 673
AclWI GGATC 2 cut(s) 393, 449
AcoI YGGCCR 2 cut(s) 394, 1044
AcsI RAATTY 1 cut(s) 25
AcyI GRCGYC 1 cut(s) 219
AfaI GTAC 3 cut(s) 167, 784, 1019
AfiI CCNNNNNNNGG 2 cut(s) 632, 991
AflII CTTAAG 1 cut(s) 1064
AflIII ACRYGT 4 cut(s) 162, 247, 573, 919
AgsI TTSAA 2 cut(s) 697, 817
AjnI CCWGG 3 cut(s) 120, 523, 893
AluBI AGCT 7 cut(s) 13, 118, 127, 190, 208, 594, 842
AluI AGCT 7 cut(s) 13, 118, 127, 190, 208, 594, 842
Alw21I GWGCWC 3 cut(s) 15, 120, 129
Alw26I GTCTC 5 cut(s) 109, 153, 321, 364, 792
AlwI GGATC 2 cut(s) 393, 449
AlwNI CAGNNNCTG 1 cut(s) 470
Ama87I CYCGRG 2 cut(s) 86, 128
AoxI GGCC 4 cut(s) 93, 394, 634, 1044
ApoI RAATTY 1 cut(s) 25
AseI ATTAAT 1 cut(s) 1058
AspA2I CCTAGG 1 cut(s) 427
AspLEI GCGC 3 cut(s) 143, 355, 377
AspS9I GGNCC 3 cut(s) 19, 94, 238
AsuHPI GGTGA 1 cut(s) 268
AvaI CYCGRG 2 cut(s) 86, 128
AvaII GGWCC 2 cut(s) 19, 238
AvrII CCTAGG 1 cut(s) 427
AxyI CCTNAGG 1 cut(s) 489
BalI TGGCCA 1 cut(s) 1046
BanI GGYRCC 1 cut(s) 170
BanII GRGCYC 3 cut(s) 15, 120, 129
BbsI GAAGAC 1 cut(s) 570
Bbv12I GWGCWC 3 cut(s) 15, 120, 129
BccI CCATC 1 cut(s) 1036
BceAI ACGGC 2 cut(s) 184, 362
BcgI CGANNNNNNTGC 2 cut(s) 634, 668
BciT130I CCWGG 3 cut(s) 122, 525, 895
BciVI GTATCC 1 cut(s) 453
BcoDI GTCTC 5 cut(s) 109, 153, 321, 364, 792
BfaI CTAG 2 cut(s) 428, 629
BfrI CTTAAG 1 cut(s) 1064
BfuAI ACCTGC 1 cut(s) 698
BfuI GTATCC 1 cut(s) 453
BisI GCNGC 3 cut(s) 144, 184, 394
BlnI CCTAGG 1 cut(s) 427
BlsI GCNGC 3 cut(s) 145, 185, 395
Bme1390I CCNGG 3 cut(s) 122, 525, 895
Bme18I GGWCC 2 cut(s) 19, 238
BmeT110I CYCGRG 2 cut(s) 86, 128
BmgT120I GGNCC 3 cut(s) 19, 94, 238
BmiI GGNNCC 3 cut(s) 96, 172, 240
BmrFI CCNGG 3 cut(s) 122, 525, 895
BmsI GCATC 1 cut(s) 47
BpiI GAAGAC 1 cut(s) 570
BplI GAGNNNNNCTC 2 cut(s) 402, 434
BpmI CTGGAG 2 cut(s) 104, 388
BpuEI CTTGAG 1 cut(s) 182
BsaBI GATNNNNATC 1 cut(s) 403
BsaHI GRCGYC 1 cut(s) 219
BsaJI CCNNGG 7 cut(s) 60, 111, 174, 427, 524, 652, 894
BsaWI WCCGGW 1 cut(s) 844
BsaXI ACNNNNNCTCC 2 cut(s) 668, 698
Bsc4I CCNNNNNNNGG 2 cut(s) 632, 991
Bse118I RCCGGY 2 cut(s) 34, 91
Bse1I ACTGG 2 cut(s) 854, 1047
Bse21I CCTNAGG 1 cut(s) 489
Bse8I GATNNNNATC 1 cut(s) 403
BseBI CCWGG 3 cut(s) 122, 525, 895
BseDI CCNNGG 7 cut(s) 60, 111, 174, 427, 524, 652, 894
BseGI GGATG 2 cut(s) 686, 1009
BseJI GATNNNNATC 1 cut(s) 403
BseLI CCNNNNNNNGG 2 cut(s) 632, 991
BseMII CTCAG 1 cut(s) 480
BseNI ACTGG 2 cut(s) 854, 1047
BseRI GAGGAG 6 cut(s) 40, 43, 68, 128, 260, 263
BseX3I CGGCCG 1 cut(s) 394
BsgI GTGCAG 1 cut(s) 738
Bsh1236I CGCG 5 cut(s) 164, 183, 249, 353, 375
Bsh1285I CGRYCG 2 cut(s) 70, 397
BshFI GGCC 4 cut(s) 95, 396, 636, 1046
BshNI GGYRCC 1 cut(s) 170
BsiEI CGRYCG 2 cut(s) 70, 397
BsiHKAI GWGCWC 3 cut(s) 15, 120, 129
BsiHKCI CYCGRG 2 cut(s) 86, 128
BsiSI CCGG 3 cut(s) 35, 92, 845
BsiWI CGTACG 1 cut(s) 165
BslFI GGGAC 3 cut(s) 192, 224, 911
BslI CCNNNNNNNGG 2 cut(s) 632, 991
BsmAI GTCTC 5 cut(s) 109, 153, 321, 364, 792
BsmBI CGTCTC 4 cut(s) 109, 153, 321, 364
BsmFI GGGAC 3 cut(s) 192, 224, 911
BsnI GGCC 4 cut(s) 95, 396, 636, 1046
BsoBI CYCGRG 2 cut(s) 86, 128
Bsp1286I GDGCHC 3 cut(s) 15, 120, 129
Bsp143I GATC 3 cut(s) 398, 441, 608
Bsp19I CCATGG 1 cut(s) 174
BspACI CCGC 9 cut(s) 57, 70, 74, 108, 144, 154, 183, 393, 673
BspANI GGCC 4 cut(s) 95, 396, 636, 1046
BspCNI CTCAG 1 cut(s) 481
BspFNI CGCG 5 cut(s) 164, 183, 249, 353, 375
BspLI GGNNCC 3 cut(s) 96, 172, 240
BspMI ACCTGC 1 cut(s) 698
BspPI GGATC 2 cut(s) 393, 449
BspQI GCTCTTC 2 cut(s) 314, 425
BspT107I GGYRCC 1 cut(s) 170
BspTI CTTAAG 1 cut(s) 1064
BsrBI CCGCTC 1 cut(s) 76
BsrFI RCCGGY 2 cut(s) 34, 91
BsrI ACTGG 2 cut(s) 854, 1047
BssAI RCCGGY 2 cut(s) 34, 91
BssECI CCNNGG 7 cut(s) 60, 111, 174, 427, 524, 652, 894
BssMI GATC 3 cut(s) 398, 441, 608
BssNI GRCGYC 1 cut(s) 219
BssT1I CCWWGG 3 cut(s) 60, 174, 427
Bst2UI CCWGG 3 cut(s) 122, 525, 895
Bst4CI ACNGT 1 cut(s) 1017
Bst6I CTCTTC 4 cut(s) 281, 314, 319, 425
BstACI GRCGYC 1 cut(s) 219
BstAFI CTTAAG 1 cut(s) 1064
BstC8I GCNNGC 4 cut(s) 74, 93, 188, 305
BstDEI CTNAG 2 cut(s) 489, 863
BstDSI CCRYGG 1 cut(s) 174
BstEII GGTNACC 1 cut(s) 969
BstF5I GGATG 2 cut(s) 686, 1009
BstFNI CGCG 5 cut(s) 164, 183, 249, 353, 375
BstHHI GCGC 3 cut(s) 143, 355, 377
BstKTI GATC 3 cut(s) 401, 444, 611
BstMAI GTCTC 5 cut(s) 109, 153, 321, 364, 792
BstMBI GATC 3 cut(s) 398, 441, 608
BstMCI CGRYCG 2 cut(s) 70, 397
BstMWI GCNNNNNNNGC 4 cut(s) 124, 372, 642, 833
BstNI CCWGG 3 cut(s) 122, 525, 895
BstNSI RCATGY 2 cut(s) 577, 923
BstPI GGTNACC 1 cut(s) 969
BstSCI CCNGG 3 cut(s) 120, 523, 893
BstUI CGCG 5 cut(s) 164, 183, 249, 353, 375
BstV2I GAAGAC 1 cut(s) 570
BstZI CGGCCG 1 cut(s) 394
Bsu36I CCTNAGG 1 cut(s) 489
BsuI GTATCC 1 cut(s) 453
BsuRI GGCC 4 cut(s) 95, 396, 636, 1046
BtgI CCRYGG 1 cut(s) 174
BtsCI GGATG 2 cut(s) 686, 1009
BtsIMutI CAGTG 2 cut(s) 861, 1054
BveI ACCTGC 1 cut(s) 698
Cac8I GCNNGC 4 cut(s) 74, 93, 188, 305
CaiI CAGNNNCTG 1 cut(s) 470
CfoI GCGC 3 cut(s) 143, 355, 377
Cfr10I RCCGGY 2 cut(s) 34, 91
Cfr13I GGNCC 3 cut(s) 19, 94, 238
CseI GACGC 5 cut(s) 170, 189, 208, 274, 381
Csp6I GTAC 3 cut(s) 166, 783, 1018
CviAII CATG 7 cut(s) 175, 262, 358, 415, 545, 574, 920
CviQI GTAC 3 cut(s) 166, 783, 1018
DdeI CTNAG 2 cut(s) 489, 863
DpnI GATC 3 cut(s) 400, 443, 610
DpnII GATC 3 cut(s) 398, 441, 608
DraI TTTAAA 1 cut(s) 946
EaeI YGGCCR 2 cut(s) 394, 1044
EagI CGGCCG 1 cut(s) 394
Eam1104I CTCTTC 4 cut(s) 281, 314, 319, 425
EarI CTCTTC 4 cut(s) 281, 314, 319, 425
EciI GGCGGA 2 cut(s) 46, 97
Ecl136II GAGCTC 3 cut(s) 13, 118, 127
EclXI CGGCCG 1 cut(s) 394
Eco130I CCWWGG 3 cut(s) 60, 174, 427
Eco24I GRGCYC 3 cut(s) 15, 120, 129
Eco32I GATATC 3 cut(s) 562, 869, 906
Eco47I GGWCC 2 cut(s) 19, 238
Eco52I CGGCCG 1 cut(s) 394
Eco53kI GAGCTC 3 cut(s) 13, 118, 127
Eco81I CCTNAGG 1 cut(s) 489
Eco88I CYCGRG 2 cut(s) 86, 128
Eco91I GGTNACC 1 cut(s) 969
EcoICRI GAGCTC 3 cut(s) 13, 118, 127
EcoO109I RGGNCCY 1 cut(s) 19
EcoO65I GGTNACC 1 cut(s) 969
EcoRI GAATTC 1 cut(s) 25
EcoRII CCWGG 3 cut(s) 120, 523, 893
EcoRV GATATC 3 cut(s) 562, 869, 906
EcoT14I CCWWGG 3 cut(s) 60, 174, 427
EcoT38I GRGCYC 3 cut(s) 15, 120, 129
ErhI CCWWGG 3 cut(s) 60, 174, 427
Esp3I CGTCTC 4 cut(s) 109, 153, 321, 364
FaeI CATG 7 cut(s) 178, 265, 361, 418, 548, 577, 923
FaqI GGGAC 3 cut(s) 192, 224, 911
FatI CATG 7 cut(s) 174, 261, 357, 414, 544, 573, 919
FauI CCCGC 2 cut(s) 81, 666
Fnu4HI GCNGC 3 cut(s) 144, 184, 394
FokI GGATG 2 cut(s) 673, 1016
FriOI GRGCYC 3 cut(s) 15, 120, 129
Fsp4HI GCNGC 3 cut(s) 144, 184, 394
FspBI CTAG 2 cut(s) 428, 629
GlaI GCGC 3 cut(s) 142, 354, 376
GluI GCNGC 3 cut(s) 144, 184, 394
GsuI CTGGAG 2 cut(s) 104, 388
HaeIII GGCC 4 cut(s) 95, 396, 636, 1046
HapII CCGG 3 cut(s) 35, 92, 845
HgaI GACGC 5 cut(s) 170, 189, 208, 274, 381
HhaI GCGC 3 cut(s) 143, 355, 377
Hin1I GRCGYC 1 cut(s) 219
Hin1II CATG 7 cut(s) 178, 265, 361, 418, 548, 577, 923
Hin6I GCGC 3 cut(s) 141, 353, 375
HinP1I GCGC 3 cut(s) 141, 353, 375
HinfI GANTC 6 cut(s) 5, 322, 411, 677, 859, 899
HpaII CCGG 3 cut(s) 35, 92, 845
HphI GGTGA 1 cut(s) 268
Hpy188I TCNGA 7 cut(s) 10, 321, 459, 591, 691, 744, 1034
Hpy188III TCNNGA 6 cut(s) 130, 241, 326, 408, 731, 817
Hpy99I CGWCG 1 cut(s) 224
HpyAV CCTTC 2 cut(s) 773, 996
HpyCH4III ACNGT 1 cut(s) 1017
HpyCH4IV ACGT 1 cut(s) 150
HpyCH4V TGCA 3 cut(s) 303, 707, 755
HpyF10VI GCNNNNNNNGC 4 cut(s) 124, 372, 642, 833
HpyF3I CTNAG 2 cut(s) 489, 863
HpySE526I ACGT 1 cut(s) 150
Hsp92I GRCGYC 1 cut(s) 219
Hsp92II CATG 7 cut(s) 178, 265, 361, 418, 548, 577, 923
HspAI GCGC 3 cut(s) 141, 353, 375
KroI GCCGGC 1 cut(s) 91
KroNI GCCGGC 1 cut(s) 93
Kzo9I GATC 3 cut(s) 398, 441, 608
LguI GCTCTTC 2 cut(s) 314, 425
LmnI GCTCC 9 cut(s) 10, 81, 115, 123, 124, 213, 273, 824, 847
LweI GCATC 1 cut(s) 47
MaeI CTAG 2 cut(s) 428, 629
MaeII ACGT 1 cut(s) 150
MaeIII GTNAC 4 cut(s) 229, 715, 961, 969
MalI GATC 3 cut(s) 400, 443, 610
MbiI CCGCTC 1 cut(s) 76
MboI GATC 3 cut(s) 398, 441, 608
MhlI GDGCHC 3 cut(s) 15, 120, 129
MlsI TGGCCA 1 cut(s) 1046
MluCI AATT 5 cut(s) 25, 762, 941, 1037, 1059
MluI ACGCGT 2 cut(s) 162, 247
MluNI TGGCCA 1 cut(s) 1046
MlyI GAGTC 3 cut(s) 420, 686, 893
MmeI TCCRAC 2 cut(s) 507, 714
Mox20I TGGCCA 1 cut(s) 1046
MroNI GCCGGC 1 cut(s) 91
MscI TGGCCA 1 cut(s) 1046
MseI TTAA 3 cut(s) 945, 1058, 1065
Msp20I TGGCCA 1 cut(s) 1046
MspA1I CMGCKG 1 cut(s) 673
MspCI CTTAAG 1 cut(s) 1064
MspI CCGG 3 cut(s) 35, 92, 845
MspR9I CCNGG 3 cut(s) 122, 525, 895
MvaI CCWGG 3 cut(s) 122, 525, 895
MvnI CGCG 5 cut(s) 164, 183, 249, 353, 375
MwoI GCNNNNNNNGC 4 cut(s) 124, 372, 642, 833
NaeI GCCGGC 1 cut(s) 93
NcoI CCATGG 1 cut(s) 174
NdeII GATC 3 cut(s) 398, 441, 608
NgoMIV GCCGGC 1 cut(s) 91
NlaIII CATG 7 cut(s) 178, 265, 361, 418, 548, 577, 923
NlaIV GGNNCC 3 cut(s) 96, 172, 240
NmeAIII GCCGAG 2 cut(s) 136, 195
NmuCI GTSAC 3 cut(s) 229, 715, 961
NspI RCATGY 2 cut(s) 577, 923
PaeR7I CTCGAG 1 cut(s) 128
PaqCI CACCTGC 1 cut(s) 698
PciI ACATGT 2 cut(s) 573, 919
PciSI GCTCTTC 2 cut(s) 314, 425
PdiI GCCGGC 1 cut(s) 93
PfeI GAWTC 3 cut(s) 5, 322, 859
Pfl23II CGTACG 1 cut(s) 165
PfoI TCCNGGA 1 cut(s) 120
PkrI GCNGC 3 cut(s) 145, 185, 395
PleI GAGTC 3 cut(s) 419, 685, 893
PpsI GAGTC 3 cut(s) 419, 685, 893
PpuMI RGGWCCY 1 cut(s) 19
PscI ACATGT 2 cut(s) 573, 919
PshBI ATTAAT 1 cut(s) 1058
Psp124BI GAGCTC 3 cut(s) 15, 120, 129
Psp5II RGGWCCY 1 cut(s) 19
Psp6I CCWGG 3 cut(s) 120, 523, 893
PspEI GGTNACC 1 cut(s) 969
PspGI CCWGG 3 cut(s) 120, 523, 893
PspLI CGTACG 1 cut(s) 165
PspN4I GGNNCC 3 cut(s) 96, 172, 240
PspPI GGNCC 3 cut(s) 19, 94, 238
PspPPI RGGWCCY 1 cut(s) 19
PstNI CAGNNNCTG 1 cut(s) 470
RsaI GTAC 3 cut(s) 167, 784, 1019
RsaNI GTAC 3 cut(s) 166, 783, 1018
SacI GAGCTC 3 cut(s) 15, 120, 129
SapI GCTCTTC 2 cut(s) 314, 425
SaqAI TTAA 3 cut(s) 945, 1058, 1065
SatI GCNGC 3 cut(s) 144, 184, 394
Sau3AI GATC 3 cut(s) 398, 441, 608
Sau96I GGNCC 3 cut(s) 19, 94, 238
SchI GAGTC 3 cut(s) 420, 686, 893
ScrFI CCNGG 3 cut(s) 122, 525, 895
SduI GDGCHC 3 cut(s) 15, 120, 129
SfaNI GCATC 1 cut(s) 47
Sfr274I CTCGAG 1 cut(s) 128
SinI GGWCC 2 cut(s) 19, 238
SlaI CTCGAG 1 cut(s) 128
SmlI CTYRAG 4 cut(s) 14, 128, 197, 1064
SmoI CTYRAG 4 cut(s) 14, 128, 197, 1064
Sse9I AATT 5 cut(s) 25, 762, 941, 1037, 1059
SsiI CCGC 9 cut(s) 57, 70, 74, 108, 144, 154, 183, 393, 673
SspMI CTAG 2 cut(s) 428, 629
SstI GAGCTC 3 cut(s) 15, 120, 129
StyD4I CCNGG 3 cut(s) 120, 523, 893
StyI CCWWGG 3 cut(s) 60, 174, 427
TaaI ACNGT 1 cut(s) 1017
TaiI ACGT 1 cut(s) 153
TaqI TCGA 4 cut(s) 129, 325, 730, 902
TasI AATT 5 cut(s) 25, 762, 941, 1037, 1059
TauI GCSGC 3 cut(s) 146, 186, 396
TfiI GAWTC 3 cut(s) 5, 322, 859
Tru1I TTAA 3 cut(s) 945, 1058, 1065
Tru9I TTAA 3 cut(s) 945, 1058, 1065
TscAI CASTG 2 cut(s) 861, 1054
TseFI GTSAC 3 cut(s) 229, 715, 961
Tsp45I GTSAC 3 cut(s) 229, 715, 961
TspGWI ACGGA 1 cut(s) 91
TspRI CASTG 2 cut(s) 861, 1054
Vha464I CTTAAG 1 cut(s) 1064
VpaK11BI GGWCC 2 cut(s) 19, 238
VspI ATTAAT 1 cut(s) 1058
XapI RAATTY 1 cut(s) 25
XceI RCATGY 2 cut(s) 577, 923
XcmI CCANNNNNNNNNTGG 1 cut(s) 610
XhoI CTCGAG 1 cut(s) 128
XmaJI CCTAGG 1 cut(s) 427
XspI CTAG 2 cut(s) 428, 629
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.